Rh5DG536600

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007599
Physical Location & Seq
Reverse (-)
82785242 .. 82788751
3510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG536600.1

Sequence Viewer

Length: 1326 bp
ATGGCTAGCGCCCGAGACCGAGACCGAGACGACTCCTTGCTCCCATTTACGACGCCCCCGTCCACGTCGTCTTCCCCGATTGTGATATCCGACCCGCTCGACGGGTTCCTCTCCGACCCGAATTCCCACATCGGGACCAGCGCCTCCGGGAGCTTCCAGAACGAGGGTTTGTTGGCAGATACTAGCGCGAGCGGCAGCGACGCCGAGTTTGGGTTTTCGAGGGGCGAGTTCCGGACGAGTCAACTCGCCGGCACGGTGGAGTTTTATCAGAGGCATGTGTTTTTGTGCTACAAGAACCCGCAGGTGTGGCCGCCGAGGATTGAGGCGGCGGATTTTGATCGGCTGCCGAGGCTGCTCCACGCGGCGGTGATGGCGAGGAGGGTGGATATGGGGAAAGTGACTCGCTTAACAATATGTGAAGGGCACGATGGAACTGAGACTTCAAATGGTGATGTATTAATTTTCCCCGACATGATCAGATACAGGAGATTGACACATTTTGATGTTGACACATTCGTTGAAGAAGTTCTTGTGAAGGATGGTGAGTGGCTGCCTGGAACTCCTGAAACTCTGAGGGGTTCATACATATTTGTATGTTCTCATGGGTCCAGGGATCGCCGTTGTGGAGTCTGTGGGCCTCCCTTGATCAATAGATTCAGAGAAGAGATAGATTTGCATGGTCTACAAGGTAAAGTGTCTGTTAGGCCATGTTCACACATTGGGGGGCATAAGTATGCAGGAAATGTAATTATATTTGGATCAAATATCAACAAAGAAGTCACTGGCCACTGGTATGGATATGTTGCTCCAGAAGATGTACCTCAATTGCTTGAGCAGCATATTGGGAGAGGAGAAATTGTAGACTGGCTATGGAGGGGTCAAATGGGTTTATCAGAAGAACAACAGAAGAAATCTCAAGAACTAAGGCTCCAGCCTAATGGCGAGTCAAATGTGGAGAAAAGAAGTACAGAGCTGACACAAACGAAGGAAAGGGGGATGAATACTGGTCCATGCAGATCTCAAGTTGAAATTGGGGGATGTTGCCAGGAAAACGGAAGCTCTTCTTGCTGCCAGAACACTTCGTTAACAGATAGGATAAGTAGTCCTGATTTGAATATGATGGCAACACAAGCGACATCTGAAAAGAAAAAGAGCAGTAGCAAACTACTTTCACGCAGAAGCAAAGCTGCATCTGCTCGAAAGTTTTGTGCTATGCCAACATGGTCTGAGAGCTGGGAGCGGGAAGATACATATGCAGCTTTGGCTATTGTTTGTGCTGCTGTGTCGGTTGGTGTTGCTTACAGTTGCTACAAACAGTTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

441

Amino Acids

48.88

Weight (kDa)

6.29

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 82 - 293 1.1e-45 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
AasI GACNNNNNNGTC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 292
AccBSI CCGCTC 3 cut(s) 97, 192, 1240
AccI GTMKAC 2 cut(s) 682, 861
AccII CGCG 2 cut(s) 188, 362
AccIII TCCGGA 1 cut(s) 231
AciI CCGC 9 cut(s) 95, 192, 299, 311, 326, 329, 362, 365, 1240
AclWI GGATC 2 cut(s) 621, 766
AcoI YGGCCR 2 cut(s) 308, 784
AcsI RAATTY 1 cut(s) 121
AcyI GRCGYC 2 cut(s) 53, 201
AfaI GTAC 2 cut(s) 819, 967
AfiI CCNNNNNNNGG 5 cut(s) 101, 132, 163, 210, 364
AgsI TTSAA 4 cut(s) 444, 521, 1028, 1114
AjiI CACGTC 1 cut(s) 66
AjnI CCWGG 3 cut(s) 553, 608, 1044
AloI GAACNNNNNNTCC 2 cut(s) 912, 944
AluBI AGCT 6 cut(s) 153, 973, 1059, 1187, 1233, 1259
AluI AGCT 6 cut(s) 153, 973, 1059, 1187, 1233, 1259
Alw26I GTCTC 4 cut(s) 9, 15, 21, 431
AlwI GGATC 2 cut(s) 621, 766
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 4 cut(s) 308, 635, 704, 784
ApeKI GCWGC 9 cut(s) 195, 343, 352, 550, 835, 1068, 1187, 1256, 1277
ApoI RAATTY 1 cut(s) 121
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 2 cut(s) 525, 1060
AspLEI GCGC 3 cut(s) 11, 143, 188
AspS9I GGNCC 4 cut(s) 135, 606, 635, 1007
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 3 cut(s) 379, 461, 554
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 3 cut(s) 135, 606, 1007
BaeGI GKGCMC 1 cut(s) 426
BalI TGGCCA 1 cut(s) 786
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 9 cut(s) 207, 330, 339, 537, 847, 1055, 1174, 1264, 1268
BccI CCATC 4 cut(s) 364, 422, 533, 1114
BceAI ACGGC 1 cut(s) 603
BciT130I CCWGG 3 cut(s) 555, 610, 1046
BclI TGATCA 2 cut(s) 474, 645
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 4 cut(s) 9, 15, 21, 431
BfaI CTAG 2 cut(s) 6, 183
BfoI RGCGCY 2 cut(s) 12, 144
BfuAI ACCTGC 1 cut(s) 292
BglII AGATCT 1 cut(s) 1016
Bme1390I CCNGG 4 cut(s) 148, 555, 610, 1046
Bme18I GGWCC 3 cut(s) 135, 606, 1007
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 66
BmgT120I GGNCC 4 cut(s) 135, 606, 635, 1007
BmiI GGNNCC 4 cut(s) 107, 136, 607, 929
BmrFI CCNGG 4 cut(s) 148, 555, 610, 1046
BmsI GCATC 1 cut(s) 1199
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 2 cut(s) 792, 914
BpuEI CTTGAG 3 cut(s) 851, 900, 1005
BpuMI CCSGG 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 2 cut(s) 53, 201
BsaI GGTCTC 2 cut(s) 9, 15
BsaJI CCNNGG 3 cut(s) 314, 347, 609
BsaWI WCCGGW 1 cut(s) 231
BsaXI ACNNNNNCTCC 4 cut(s) 843, 873, 912, 942
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 132, 163, 210, 364
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 4 cut(s) 787, 794, 869, 1009
Bse8I GATNNNNATC 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 3 cut(s) 555, 610, 1046
BseDI CCNNGG 3 cut(s) 314, 347, 609
BseGI GGATG 3 cut(s) 544, 1002, 1043
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 5 cut(s) 101, 132, 163, 210, 364
BseMII CTCAG 3 cut(s) 426, 563, 1218
BseNI ACTGG 4 cut(s) 787, 794, 869, 1009
BseRI GAGGAG 2 cut(s) 391, 864
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 9 cut(s) 207, 330, 339, 537, 847, 1055, 1174, 1264, 1268
BseYI CCCAGC 1 cut(s) 1233
Bsh1236I CGCG 2 cut(s) 188, 362
BshFI GGCC 4 cut(s) 310, 637, 706, 786
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 147, 232, 249
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 5 cut(s) 101, 132, 163, 210, 364
BsmAI GTCTC 4 cut(s) 9, 15, 21, 431
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 4 cut(s) 310, 637, 706, 786
Bso31I GGTCTC 2 cut(s) 9, 15
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 426
Bsp13I TCCGGA 1 cut(s) 231
Bsp143I GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
BspACI CCGC 9 cut(s) 95, 192, 299, 311, 326, 329, 362, 365, 1240
BspANI GGCC 4 cut(s) 310, 637, 706, 786
BspCNI CTCAG 3 cut(s) 427, 564, 1219
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 2 cut(s) 188, 362
BspLI GGNNCC 4 cut(s) 107, 136, 607, 929
BspMI ACCTGC 1 cut(s) 292
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 621, 766
BspQI GCTCTTC 1 cut(s) 1066
BspTNI GGTCTC 2 cut(s) 9, 15
BsrBI CCGCTC 3 cut(s) 97, 192, 1240
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 4 cut(s) 787, 794, 869, 1009
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 314, 347, 609
BssMI GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
BssNI GRCGYC 2 cut(s) 53, 201
Bst2UI CCWGG 3 cut(s) 555, 610, 1046
Bst4CI ACNGT 3 cut(s) 256, 1304, 1317
Bst6I CTCTTC 2 cut(s) 657, 1066
BstACI GRCGYC 2 cut(s) 53, 201
BstC8I GCNNGC 3 cut(s) 7, 190, 250
BstDEI CTNAG 4 cut(s) 435, 572, 923, 1227
BstF5I GGATG 3 cut(s) 544, 1002, 1043
BstFNI CGCG 2 cut(s) 188, 362
BstH2I RGCGCY 2 cut(s) 12, 144
BstHHI GCGC 3 cut(s) 11, 143, 188
BstKTI GATC 6 cut(s) 340, 477, 616, 648, 761, 1019
BstMAI GTCTC 4 cut(s) 9, 15, 21, 431
BstMBI GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
BstNI CCWGG 3 cut(s) 555, 610, 1046
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 4 cut(s) 146, 553, 608, 1044
BstSLI GKGCMC 1 cut(s) 426
BstUI CGCG 2 cut(s) 188, 362
BstV1I GCAGC 9 cut(s) 207, 330, 339, 537, 847, 1055, 1174, 1264, 1268
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 1016
BstXI CCANNNNNNTGG 2 cut(s) 794, 938
BstYI RGATCY 1 cut(s) 1016
BsuRI GGCC 4 cut(s) 310, 637, 706, 786
BtrI CACGTC 1 cut(s) 66
BtsCI GGATG 3 cut(s) 544, 1002, 1043
BtsIMutI CAGTG 2 cut(s) 780, 787
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 3 cut(s) 7, 190, 250
CfoI GCGC 3 cut(s) 11, 143, 188
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 4 cut(s) 135, 606, 635, 1007
CseI GACGC 2 cut(s) 61, 209
Csp6I GTAC 2 cut(s) 818, 966
CviAII CATG 7 cut(s) 275, 472, 602, 677, 708, 1011, 1221
CviQI GTAC 2 cut(s) 818, 966
DdeI CTNAG 4 cut(s) 435, 572, 923, 1227
DpnI GATC 6 cut(s) 339, 476, 615, 647, 760, 1018
DpnII GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
DrdI GACNNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 2 cut(s) 308, 784
Eam1104I CTCTTC 2 cut(s) 657, 1066
EarI CTCTTC 2 cut(s) 657, 1066
EciI GGCGGA 1 cut(s) 344
Eco31I GGTCTC 2 cut(s) 9, 15
Eco32I GATATC 1 cut(s) 87
Eco47I GGWCC 3 cut(s) 135, 606, 1007
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 553, 608, 1044
EcoRV GATATC 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 7 cut(s) 278, 475, 605, 680, 711, 1014, 1224
FalI AAGNNNNNCTT 4 cut(s) 513, 545, 1048, 1080
FaqI GGGAC 1 cut(s) 148
FatI CATG 7 cut(s) 274, 471, 601, 676, 707, 1010, 1220
FauI CCCGC 3 cut(s) 102, 306, 1233
FauNDI CATATG 1 cut(s) 1252
FbaI TGATCA 2 cut(s) 474, 645
FblI GTMKAC 2 cut(s) 682, 861
FokI GGATG 3 cut(s) 551, 1009, 1050
FspBI CTAG 2 cut(s) 6, 183
GlaI GCGC 3 cut(s) 10, 142, 187
GsaI CCCAGC 1 cut(s) 1237
GsuI CTGGAG 2 cut(s) 792, 914
HaeII RGCGCY 2 cut(s) 12, 144
HaeIII GGCC 4 cut(s) 310, 637, 706, 786
HapII CCGG 3 cut(s) 147, 232, 249
HgaI GACGC 2 cut(s) 61, 209
HhaI GCGC 3 cut(s) 11, 143, 188
Hin1I GRCGYC 2 cut(s) 53, 201
Hin1II CATG 7 cut(s) 278, 475, 605, 680, 711, 1014, 1224
Hin6I GCGC 3 cut(s) 9, 141, 186
HinP1I GCGC 3 cut(s) 9, 141, 186
HincII GTYRAC 3 cut(s) 242, 508, 1086
HindII GTYRAC 3 cut(s) 242, 508, 1086
HinfI GANTC 6 cut(s) 32, 238, 400, 627, 654, 944
HpaI GTTAAC 1 cut(s) 1086
HpaII CCGG 3 cut(s) 147, 232, 249
HphI GGTGA 3 cut(s) 379, 461, 554
Hpy166II GTNNAC 7 cut(s) 63, 242, 508, 683, 713, 862, 1086
Hpy188I TCNGA 9 cut(s) 91, 115, 270, 479, 573, 659, 895, 1141, 1228
Hpy188III TCNNGA 7 cut(s) 133, 157, 232, 563, 809, 917, 1106
Hpy8I GTNNAC 7 cut(s) 63, 242, 508, 683, 713, 862, 1086
Hpy99I CGWCG 4 cut(s) 55, 70, 104, 203
HpyAV CCTTC 3 cut(s) 413, 529, 979
HpyCH4III ACNGT 3 cut(s) 256, 1304, 1317
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 5 cut(s) 676, 737, 1014, 1190, 1256
HpyF3I CTNAG 4 cut(s) 435, 572, 923, 1227
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 2 cut(s) 53, 201
Hsp92II CATG 7 cut(s) 278, 475, 605, 680, 711, 1014, 1224
HspAI GCGC 3 cut(s) 9, 141, 186
Kpn2I TCCGGA 1 cut(s) 231
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Ksp22I TGATCA 2 cut(s) 474, 645
KspAI GTTAAC 1 cut(s) 1086
Kzo9I GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
LguI GCTCTTC 1 cut(s) 1066
LmnI GCTCC 6 cut(s) 45, 150, 360, 811, 933, 1237
Lsp1109I GCAGC 9 cut(s) 207, 330, 339, 537, 847, 1055, 1174, 1264, 1268
LweI GCATC 1 cut(s) 1199
MaeI CTAG 2 cut(s) 6, 183
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 2 cut(s) 397, 778
MalI GATC 6 cut(s) 339, 476, 615, 647, 760, 1018
MbiI CCGCTC 3 cut(s) 97, 192, 1240
MboI GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
MboII GAAGA 8 cut(s) 63, 533, 674, 824, 908, 919, 1053, 1256
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 1 cut(s) 1016
MhlI GDGCHC 1 cut(s) 426
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 6 cut(s) 121, 459, 747, 824, 855, 1029
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 5 cut(s) 26, 247, 394, 636, 953
MmeI TCCRAC 2 cut(s) 114, 138
Mox20I TGGCCA 1 cut(s) 786
MroI TCCGGA 1 cut(s) 231
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 2 cut(s) 525, 1060
MscI TGGCCA 1 cut(s) 786
MseI TTAA 3 cut(s) 407, 458, 1085
MslI CAYNNNNRTG 3 cut(s) 501, 732, 792
Msp20I TGGCCA 1 cut(s) 786
MspI CCGG 3 cut(s) 147, 232, 249
MspR9I CCNGG 4 cut(s) 148, 555, 610, 1046
MunI CAATTG 1 cut(s) 824
MvaI CCWGG 3 cut(s) 555, 610, 1046
MvnI CGCG 2 cut(s) 188, 362
NaeI GCCGGC 1 cut(s) 250
NciI CCSGG 1 cut(s) 148
NdeI CATATG 1 cut(s) 1252
NdeII GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
NgoMIV GCCGGC 1 cut(s) 248
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 7 cut(s) 278, 475, 605, 680, 711, 1014, 1224
NlaIV GGNNCC 4 cut(s) 107, 136, 607, 929
NmeAIII GCCGAG 3 cut(s) 229, 339, 372
NmuCI GTSAC 2 cut(s) 397, 778
NspI RCATGY 1 cut(s) 278
PaqCI CACCTGC 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 1066
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 74
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 2 cut(s) 525, 1060
PfeI GAWTC 1 cut(s) 654
PfoI TCCNGGA 1 cut(s) 146
PleI GAGTC 5 cut(s) 26, 246, 394, 635, 952
PpsI GAGTC 5 cut(s) 26, 246, 394, 635, 952
PshBI ATTAAT 1 cut(s) 458
Psp6I CCWGG 3 cut(s) 553, 608, 1044
PspFI CCCAGC 1 cut(s) 1233
PspGI CCWGG 3 cut(s) 553, 608, 1044
PspN4I GGNNCC 4 cut(s) 107, 136, 607, 929
PspPI GGNCC 4 cut(s) 135, 606, 635, 1007
PsuI RGATCY 1 cut(s) 1016
RsaI GTAC 2 cut(s) 819, 967
RsaNI GTAC 2 cut(s) 818, 966
RseI CAYNNNNRTG 3 cut(s) 501, 732, 792
SapI GCTCTTC 1 cut(s) 1066
SaqAI TTAA 3 cut(s) 407, 458, 1085
Sau3AI GATC 6 cut(s) 337, 474, 613, 645, 758, 1016
Sau96I GGNCC 4 cut(s) 135, 606, 635, 1007
SchI GAGTC 5 cut(s) 26, 247, 394, 636, 953
ScrFI CCNGG 4 cut(s) 148, 555, 610, 1046
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 1 cut(s) 1199
SinI GGWCC 3 cut(s) 135, 606, 1007
SmiMI CAYNNNNRTG 3 cut(s) 501, 732, 792
SmlI CTYRAG 3 cut(s) 830, 915, 1020
SmoI CTYRAG 3 cut(s) 830, 915, 1020
Sse9I AATT 6 cut(s) 121, 459, 747, 824, 855, 1029
SsiI CCGC 9 cut(s) 95, 192, 299, 311, 326, 329, 362, 365, 1240
SspMI CTAG 2 cut(s) 6, 183
StyD4I CCNGG 4 cut(s) 146, 553, 608, 1044
TaaI ACNGT 3 cut(s) 256, 1304, 1317
TaiI ACGT 1 cut(s) 68
TaqI TCGA 3 cut(s) 99, 218, 1198
TaqII GACCGA 2 cut(s) 33, 39
TasI AATT 6 cut(s) 121, 459, 747, 824, 855, 1029
TatI WGTACW 1 cut(s) 965
TauI GCSGC 4 cut(s) 195, 313, 329, 365
TfiI GAWTC 1 cut(s) 654
Tru1I TTAA 3 cut(s) 407, 458, 1085
Tru9I TTAA 3 cut(s) 407, 458, 1085
TscAI CASTG 2 cut(s) 787, 794
TseFI GTSAC 2 cut(s) 397, 778
TseI GCWGC 9 cut(s) 195, 343, 352, 550, 835, 1068, 1187, 1256, 1277
Tsp45I GTSAC 2 cut(s) 397, 778
TspDTI ATGAA 2 cut(s) 570, 1013
TspGWI ACGGA 1 cut(s) 1068
TspRI CASTG 2 cut(s) 787, 794
VpaK11BI GGWCC 3 cut(s) 135, 606, 1007
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 1 cut(s) 121
XceI RCATGY 1 cut(s) 278
XmiI GTMKAC 2 cut(s) 682, 861
XmnI GAANNNNTTC 2 cut(s) 525, 1060
XspI CTAG 2 cut(s) 6, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.