AT5G56140

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007994
Physical Location & Seq
Forward (+)
22725238 .. 22728473
3236 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56140.1

Sequence Viewer

Length: 948 bp
ATGATGATGATGTCGTCGCTTGGTGGTGGTGGTGGTGGAGGAGGAGGAAGTGGTGGTGGTATTGGTGGTGGCGGCGGAGGGAGGTTTATGACGTATTCGTCGTCTCTTTCGGTGCCTCCTTCTGCTCCTCAGTCTCCTAATTACTCCGGTGGTCTCCGATCACAGTCTTCTGTCTTCGTTGAGCAAGAGAAGTATCTTTCAGAGTTACTTGCAGAGCGTCATAAGCTTACTCCATTCCTGCCTGTGCTTCCACATGCATTTCGGCTACTAAACCAAGAAATTTTGCGTGTAACCACACTGTTGGAGAATGCTACAGTTTTAAGTCAGAGTGGGCTTGACCACCCTAGCCCTCTGGCTTCTGGAGGGATATTTCAGAATGCAAGAGCTGACATGAATGGATGGGCCTCCCAGTTTCCATCAGAAAGATCTGTTCCATCATCTCCGGGACCAAATTGGCTTAACTCACCTGGTAGTTCGTCCGGTCTCATCGCCAAGCGTACAATCAGGGTCGATATTCCAGTCGACAATTATCCGAATTTCAATTTTGTTGGTCGCCTCCTGGGTCCTAGAGGAAACTCCCTGAAGCGAGTTGAAGCAAGTACTGATTGCCGTGTTCTTATAAGAGGAAGAGGAAGTATAAAAGATCCAATTAAGGAGGAAATGATGAGAGGAAAACCGGGATACGAGCACCTAAATGAACCACTTCATATCTTAGTGGAAGCAGAATTACCGATTGAAATAGTCGATGCACGGCTCATGCAAGCTCGGGAAATACTTGACGATCTTCTTACTCCTATGGAGGAAACCCATGATATGTACAAGAAACAACAGCTGCGCGAGCTAGCATTGCTCAATGGAACTCTTCGTGAAGAAGGATCTCCAATGTCCGGTTCTGTTTCTCCTTACAATAGCCTTGGTATGAAGAGAGCCAAAACAAGAGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 STAR_dimer

Protein Analysis

315

Amino Acids

33.98

Weight (kDa)

7.87

Isoelectric Point (pI)

62.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STAR_dimer PF16544 63 - 101 1.6e-09 Homodimerisation region of STAR domain protein
KH-I_KHDC4-BBP PF22675 176 - 263 5.1e-19 KHDC4/BBP-like, KH-domain type I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 620
AasI GACNNNNNNGTC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 522
AccII CGCG 1 cut(s) 837
AciI CCGC 2 cut(s) 72, 75
AclWI GGATC 2 cut(s) 638, 883
AcsI RAATTY 2 cut(s) 279, 535
AcuI CTGAAG 1 cut(s) 602
AfaI GTAC 3 cut(s) 499, 601, 818
AfiI CCNNNNNNNGG 1 cut(s) 887
AgsI TTSAA 3 cut(s) 541, 593, 737
AjnI CCWGG 2 cut(s) 466, 558
AjuI GAANNNNNNNTTGG 2 cut(s) 874, 906
AluBI AGCT 5 cut(s) 226, 386, 764, 832, 841
AluI AGCT 5 cut(s) 226, 386, 764, 832, 841
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 4 cut(s) 108, 138, 158, 488
AlwI GGATC 2 cut(s) 638, 883
Ama87I CYCGRG 1 cut(s) 765
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 832
ApoI RAATTY 2 cut(s) 279, 535
Asp700I GAANNNNTTC 1 cut(s) 702
AspLEI GCGC 1 cut(s) 837
AspS9I GGNCC 3 cut(s) 402, 446, 563
AsuC2I CCSGG 2 cut(s) 444, 678
AsuHPI GGTGA 1 cut(s) 456
AsuNHI GCTAGC 1 cut(s) 841
AvaI CYCGRG 1 cut(s) 765
AvaII GGWCC 2 cut(s) 446, 563
BanI GGYRCC 1 cut(s) 112
BarI GAAGNNNNNNTAC 2 cut(s) 711, 743
BbsI GAAGAC 2 cut(s) 159, 166
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 1 cut(s) 819
BccI CCATC 3 cut(s) 393, 424, 442
BceAI ACGGC 2 cut(s) 594, 767
BciT130I CCWGG 2 cut(s) 468, 560
BciVI GTATCC 1 cut(s) 674
BcnI CCSGG 2 cut(s) 444, 678
BcoDI GTCTC 4 cut(s) 108, 138, 158, 488
BfaI CTAG 3 cut(s) 345, 567, 842
BfmI CTRYAG 1 cut(s) 312
BfuI GTATCC 1 cut(s) 674
BglII AGATCT 1 cut(s) 425
BisI GCNGC 2 cut(s) 73, 833
BlsI GCNGC 2 cut(s) 74, 834
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 4 cut(s) 444, 468, 560, 678
Bme18I GGWCC 2 cut(s) 446, 563
BmeT110I CYCGRG 1 cut(s) 765
BmgT120I GGNCC 3 cut(s) 402, 446, 563
BmiI GGNNCC 3 cut(s) 114, 447, 564
BmrFI CCNGG 4 cut(s) 444, 468, 560, 678
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 736
BmtI GCTAGC 1 cut(s) 845
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 2 cut(s) 159, 166
BpmI CTGGAG 1 cut(s) 381
BpuMI CCSGG 2 cut(s) 444, 678
BsaI GGTCTC 2 cut(s) 158, 488
BsaJI CCNNGG 2 cut(s) 559, 913
BsaWI WCCGGW 3 cut(s) 146, 479, 887
Bsc4I CCNNNNNNNGG 1 cut(s) 887
Bse1I ACTGG 2 cut(s) 409, 518
Bse3DI GCAATG 1 cut(s) 845
BseBI CCWGG 2 cut(s) 468, 560
BseDI CCNNGG 2 cut(s) 559, 913
BseGI GGATG 1 cut(s) 404
BseLI CCNNNNNNNGG 1 cut(s) 887
BseMI GCAATG 1 cut(s) 845
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 409, 518
BseRI GAGGAG 3 cut(s) 54, 57, 117
BseXI GCAGC 1 cut(s) 819
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 1 cut(s) 404
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 1 cut(s) 765
BsiSI CCGG 5 cut(s) 147, 443, 480, 677, 888
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 1 cut(s) 887
BsmAI GTCTC 4 cut(s) 108, 138, 158, 488
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 459
BsmI GAATGC 2 cut(s) 313, 382
BsnI GGCC 1 cut(s) 404
Bso31I GGTCTC 2 cut(s) 158, 488
BsoBI CYCGRG 1 cut(s) 765
Bsp1286I GDGCHC 1 cut(s) 690
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 5 cut(s) 158, 425, 643, 781, 875
BspACI CCGC 2 cut(s) 72, 75
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 1 cut(s) 837
BspLI GGNNCC 3 cut(s) 114, 447, 564
BspOI GCTAGC 1 cut(s) 845
BspPI GGATC 2 cut(s) 638, 883
BspT107I GGYRCC 1 cut(s) 112
BspTNI GGTCTC 2 cut(s) 158, 488
BsrDI GCAATG 1 cut(s) 845
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 409, 518
BssECI CCNNGG 2 cut(s) 559, 913
BssMI GATC 5 cut(s) 158, 425, 643, 781, 875
BssT1I CCWWGG 1 cut(s) 913
Bst2UI CCWGG 2 cut(s) 468, 560
Bst4CI ACNGT 3 cut(s) 165, 300, 316
Bst6I CTCTTC 3 cut(s) 622, 867, 917
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 3 cut(s) 762, 839, 843
BstDEI CTNAG 2 cut(s) 129, 712
BstF5I GGATG 1 cut(s) 404
BstFNI CGCG 1 cut(s) 837
BstHHI GCGC 1 cut(s) 837
BstKTI GATC 5 cut(s) 161, 428, 646, 784, 878
BstMAI GTCTC 4 cut(s) 108, 138, 158, 488
BstMBI GATC 5 cut(s) 158, 425, 643, 781, 875
BstMWI GCNNNNNNNGC 3 cut(s) 223, 838, 847
BstNI CCWGG 2 cut(s) 468, 560
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 4 cut(s) 442, 466, 558, 676
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 837
BstV1I GCAGC 1 cut(s) 819
BstV2I GAAGAC 2 cut(s) 159, 166
BstX2I RGATCY 3 cut(s) 425, 643, 875
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 3 cut(s) 425, 643, 875
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 1 cut(s) 404
BtgZI GCGATG 1 cut(s) 472
BtsCI GGATG 1 cut(s) 404
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 3 cut(s) 762, 839, 843
CfoI GCGC 1 cut(s) 837
Cfr13I GGNCC 3 cut(s) 402, 446, 563
CseI GACGC 1 cut(s) 206
CsiI ACCWGGT 1 cut(s) 466
Csp6I GTAC 3 cut(s) 498, 600, 817
CviAII CATG 4 cut(s) 254, 391, 757, 809
CviQI GTAC 3 cut(s) 498, 600, 817
DdeI CTNAG 2 cut(s) 129, 712
DpnI GATC 5 cut(s) 160, 427, 645, 783, 877
DpnII GATC 5 cut(s) 158, 425, 643, 781, 875
DrdI GACNNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 3 cut(s) 622, 867, 917
EarI CTCTTC 3 cut(s) 622, 867, 917
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 913
Eco31I GGTCTC 2 cut(s) 158, 488
Eco47I GGWCC 2 cut(s) 446, 563
Eco57I CTGAAG 1 cut(s) 602
Eco88I CYCGRG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 466, 558
EcoT14I CCWWGG 1 cut(s) 913
EcoT22I ATGCAT 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 913
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 4 cut(s) 257, 394, 760, 812
FaqI GGGAC 1 cut(s) 459
FatI CATG 4 cut(s) 253, 390, 756, 808
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 73, 833
FokI GGATG 1 cut(s) 411
Fsp4HI GCNGC 2 cut(s) 73, 833
FspBI CTAG 3 cut(s) 345, 567, 842
GlaI GCGC 1 cut(s) 836
GluI GCNGC 2 cut(s) 73, 833
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 404
HapII CCGG 5 cut(s) 147, 443, 480, 677, 888
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 837
Hin1II CATG 4 cut(s) 257, 394, 760, 812
Hin6I GCGC 1 cut(s) 835
HinP1I GCGC 1 cut(s) 835
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 224
HpaII CCGG 5 cut(s) 147, 443, 480, 677, 888
HphI GGTGA 1 cut(s) 456
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 6 cut(s) 158, 202, 327, 375, 421, 534
Hpy188III TCNNGA 3 cut(s) 360, 767, 866
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 2 cut(s) 19, 103
HpyAV CCTTC 3 cut(s) 129, 866, 935
HpyCH4III ACNGT 3 cut(s) 165, 300, 316
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 5 cut(s) 212, 257, 380, 749, 760
HpyF10VI GCNNNNNNNGC 3 cut(s) 223, 838, 847
HpyF3I CTNAG 2 cut(s) 129, 712
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 4 cut(s) 257, 394, 760, 812
HspAI GCGC 1 cut(s) 835
Kzo9I GATC 5 cut(s) 158, 425, 643, 781, 875
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 1 cut(s) 819
LweI GCATC 1 cut(s) 736
MabI ACCWGGT 1 cut(s) 466
MaeI CTAG 3 cut(s) 345, 567, 842
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 2 cut(s) 204, 289
MalI GATC 5 cut(s) 160, 427, 645, 783, 877
MboI GATC 5 cut(s) 158, 425, 643, 781, 875
MboII GAAGA 7 cut(s) 159, 166, 639, 776, 854, 881, 934
MflI RGATCY 3 cut(s) 425, 643, 875
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 8 cut(s) 139, 279, 451, 526, 535, 541, 648, 725
MmeI TCCRAC 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 259
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 3 cut(s) 320, 459, 651
MslI CAYNNNNRTG 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 832
MspI CCGG 5 cut(s) 147, 443, 480, 677, 888
MspR9I CCNGG 4 cut(s) 444, 468, 560, 678
Mva1269I GAATGC 2 cut(s) 313, 382
MvaI CCWGG 2 cut(s) 468, 560
MvnI CGCG 1 cut(s) 837
MwoI GCNNNNNNNGC 3 cut(s) 223, 838, 847
NciI CCSGG 2 cut(s) 444, 678
NdeII GATC 5 cut(s) 158, 425, 643, 781, 875
NheI GCTAGC 1 cut(s) 841
NlaIII CATG 4 cut(s) 257, 394, 760, 812
NlaIV GGNNCC 3 cut(s) 114, 447, 564
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PctI GAATGC 2 cut(s) 313, 382
PdmI GAANNNNTTC 1 cut(s) 702
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 2 cut(s) 74, 834
PpuMI RGGWCCY 1 cut(s) 563
PsiI TTATAA 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 2 cut(s) 466, 558
PspGI CCWGG 2 cut(s) 466, 558
PspN4I GGNNCC 3 cut(s) 114, 447, 564
PspPI GGNCC 3 cut(s) 402, 446, 563
PspPPI RGGWCCY 1 cut(s) 563
PsuI RGATCY 3 cut(s) 425, 643, 875
PvuII CAGCTG 1 cut(s) 832
RsaI GTAC 3 cut(s) 499, 601, 818
RsaNI GTAC 3 cut(s) 498, 600, 817
RseI CAYNNNNRTG 1 cut(s) 693
SalI GTCGAC 1 cut(s) 521
SaqAI TTAA 3 cut(s) 320, 459, 651
SatI GCNGC 2 cut(s) 73, 833
Sau3AI GATC 5 cut(s) 158, 425, 643, 781, 875
Sau96I GGNCC 3 cut(s) 402, 446, 563
ScaI AGTACT 1 cut(s) 601
ScrFI CCNGG 4 cut(s) 444, 468, 560, 678
SduI GDGCHC 1 cut(s) 690
SetI ASST 9 cut(s) 86, 95, 228, 388, 469, 693, 766, 834, 843
SexAI ACCWGGT 1 cut(s) 466
SfaNI GCATC 1 cut(s) 736
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 446, 563
SmiMI CAYNNNNRTG 1 cut(s) 693
Sse9I AATT 8 cut(s) 139, 279, 451, 526, 535, 541, 648, 725
SsiI CCGC 2 cut(s) 72, 75
SspMI CTAG 3 cut(s) 345, 567, 842
StyD4I CCNGG 4 cut(s) 442, 466, 558, 676
StyI CCWWGG 1 cut(s) 913
TaaI ACNGT 3 cut(s) 165, 300, 316
TaiI ACGT 1 cut(s) 95
TaqI TCGA 3 cut(s) 510, 522, 744
TasI AATT 8 cut(s) 139, 279, 451, 526, 535, 541, 648, 725
TatI WGTACW 2 cut(s) 599, 816
TauI GCSGC 1 cut(s) 75
Tru1I TTAA 3 cut(s) 320, 459, 651
Tru9I TTAA 3 cut(s) 320, 459, 651
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 832
TspDTI ATGAA 4 cut(s) 407, 695, 711, 935
TspRI CASTG 1 cut(s) 303
VpaK11BI GGWCC 2 cut(s) 446, 563
XapI RAATTY 2 cut(s) 279, 535
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 3 cut(s) 345, 567, 842
ZrmI AGTACT 1 cut(s) 601
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.