MD03G1041400.v1.1

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007994
Physical Location & Seq
Forward (+)
3278081 .. 3279637
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1041400.v1.1.491

Sequence Viewer

Length: 531 bp
ATGTCAGGTTTATTACAGCCTTTATCAACCCAAAACTGGCTGAGTTCTCAGAGTAGTTCATCTGGTCTGATTGTGAAGCGAACAATCCGGGTGGATATTCCAGTTGACAAATATCCTAATTTTAACTTTGTTGGGCGGTTGCTAGGCCCTAGGGGAAACTCTCTTAAGCGGGTGGAAGCAACTACTGAATGTCGTGTTCTGATCAGGGGACGTGGTAGCATAAAGGATCCAACTAGGGAAGAAATGATGAGAGGAAAACCAGGGTATGAACATCTGAACGAACCTCTTCATATTCTAGTGGAGGCAGAACTACCAGTTGAAATTATTGATGCTCGTTTATTACAAGCACGTGAGGTACTTGAAGATTTGCTCAGGCCTGTGGACGAATCTCAGGATTTCTACAAGAAACAGCAGCTGCGAGAGTTGGCAATCCTCAATGGCACACTTCGTGAAGAGGGTTCTCCAATGTCTGGTTCGGTGTCACCCTTCCACAACAGTCTTGGTATGAAGAGGGCCAAGACCAGGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 STAR_dimer

Protein Analysis

177

Amino Acids

19.81

Weight (kDa)

9.37

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH-I_KHDC4-BBP PF22675 37 - 124 2.2e-20 KHDC4/BBP-like, KH-domain type I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 470
AciI CCGC 2 cut(s) 136, 169
AclWI GGATC 2 cut(s) 221, 234
AcvI CACGTG 1 cut(s) 350
AdeI CACNNNGTG 1 cut(s) 449
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 3 cut(s) 36, 470, 522
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 2 cut(s) 320, 362
AjiI CACGTC 1 cut(s) 212
AjnI CCWGG 2 cut(s) 259, 521
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AluBI AGCT 1 cut(s) 415
AluI AGCT 1 cut(s) 415
AlwI GGATC 2 cut(s) 221, 234
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 3 cut(s) 145, 374, 513
ApeKI GCWGC 2 cut(s) 412, 415
Asp700I GAANNNNTTC 1 cut(s) 285
AspA2I CCTAGG 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 146, 513
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 474
AvrII CCTAGG 1 cut(s) 149
BamHI GGATCC 1 cut(s) 226
BbrPI CACGTG 1 cut(s) 350
BbvI GCAGC 2 cut(s) 402, 424
BciT130I CCWGG 2 cut(s) 261, 523
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 1 cut(s) 89
BfaI CTAG 4 cut(s) 143, 150, 234, 296
BfrI CTTAAG 1 cut(s) 164
BisI GCNGC 2 cut(s) 413, 416
BlnI CCTAGG 1 cut(s) 149
BlsI GCNGC 2 cut(s) 414, 417
Bme1390I CCNGG 3 cut(s) 89, 261, 523
BmgBI CACGTC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 146, 513
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 3 cut(s) 89, 261, 523
BmsI GCATC 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 149, 260, 522
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 470, 522
Bse1I ACTGG 3 cut(s) 41, 101, 314
BseBI CCWGG 2 cut(s) 261, 523
BseDI CCNNGG 3 cut(s) 149, 260, 522
BseLI CCNNNNNNNGG 3 cut(s) 36, 470, 522
BseMII CTCAG 4 cut(s) 32, 62, 385, 404
BseNI ACTGG 3 cut(s) 41, 101, 314
BseXI GCAGC 2 cut(s) 402, 424
BshFI GGCC 3 cut(s) 147, 376, 515
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 36, 470, 522
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 3 cut(s) 147, 376, 515
Bsp143I GATC 2 cut(s) 201, 226
BspACI CCGC 2 cut(s) 136, 169
BspANI GGCC 3 cut(s) 147, 376, 515
BspCNI CTCAG 4 cut(s) 33, 61, 384, 403
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 2 cut(s) 221, 234
BspTI CTTAAG 1 cut(s) 164
BsrI ACTGG 3 cut(s) 41, 101, 314
BssECI CCNNGG 3 cut(s) 149, 260, 522
BssMI GATC 2 cut(s) 201, 226
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 2 cut(s) 261, 523
Bst4CI ACNGT 1 cut(s) 497
Bst6I CTCTTC 3 cut(s) 291, 447, 503
BstAFI CTTAAG 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 350
BstDEI CTNAG 4 cut(s) 41, 48, 371, 390
BstKTI GATC 2 cut(s) 204, 229
BstMBI GATC 2 cut(s) 201, 226
BstNI CCWGG 2 cut(s) 261, 523
BstSCI CCNGG 3 cut(s) 87, 259, 521
BstV1I GCAGC 2 cut(s) 402, 424
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 3 cut(s) 147, 376, 515
BtrI CACGTC 1 cut(s) 212
CaiI CAGNNNCTG 1 cut(s) 415
Cfr13I GGNCC 2 cut(s) 146, 513
Csp6I GTAC 1 cut(s) 356
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviJI RGCY 6 cut(s) 19, 40, 147, 376, 415, 515
CviKI_1 RGCY 6 cut(s) 19, 40, 147, 376, 415, 515
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 4 cut(s) 41, 48, 371, 390
DpnI GATC 2 cut(s) 203, 228
DpnII GATC 2 cut(s) 201, 226
DraIII CACNNNGTG 1 cut(s) 449
Eam1104I CTCTTC 3 cut(s) 291, 447, 503
EarI CTCTTC 3 cut(s) 291, 447, 503
Eco130I CCWWGG 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 376
Eco72I CACGTG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 259, 521
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaiI YATR 4 cut(s) 221, 267, 291, 506
FaqI GGGAC 1 cut(s) 222
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 413, 416
Fsp4HI GCNGC 2 cut(s) 413, 416
FspBI CTAG 4 cut(s) 143, 150, 234, 296
GluI GCNGC 2 cut(s) 413, 416
HaeIII GGCC 3 cut(s) 147, 376, 515
HapII CCGG 1 cut(s) 88
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 1 cut(s) 386
HpaII CCGG 1 cut(s) 88
HphI GGTGA 1 cut(s) 474
Hpy166II GTNNAC 2 cut(s) 106, 382
Hpy188I TCNGA 4 cut(s) 51, 69, 201, 276
Hpy188III TCNNGA 2 cut(s) 392, 449
Hpy8I GTNNAC 2 cut(s) 106, 382
HpyAV CCTTC 1 cut(s) 496
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4IV ACGT 2 cut(s) 211, 349
HpyF3I CTNAG 4 cut(s) 41, 48, 371, 390
HpySE526I ACGT 2 cut(s) 211, 349
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 2 cut(s) 201, 226
Lsp1109I GCAGC 2 cut(s) 402, 424
LweI GCATC 1 cut(s) 319
MaeI CTAG 4 cut(s) 143, 150, 234, 296
MaeII ACGT 2 cut(s) 211, 349
MaeIII GTNAC 1 cut(s) 480
MalI GATC 2 cut(s) 203, 228
MboI GATC 2 cut(s) 201, 226
MboII GAAGA 5 cut(s) 251, 278, 374, 464, 520
MflI RGATCY 1 cut(s) 226
MluCI AATT 2 cut(s) 118, 321
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 7 cut(s) 245, 294, 295, 346, 443, 448, 504
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 2 cut(s) 123, 165
MspA1I CMGCKG 1 cut(s) 415
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 3 cut(s) 89, 261, 523
MvaI CCWGG 2 cut(s) 261, 523
NciI CCSGG 1 cut(s) 89
NdeII GATC 2 cut(s) 201, 226
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 480
PceI AGGCCT 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 470
PkrI GCNGC 2 cut(s) 414, 417
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
Ppu21I YACGTR 1 cut(s) 350
Psp6I CCWGG 2 cut(s) 259, 521
PspCI CACGTG 1 cut(s) 350
PspGI CCWGG 2 cut(s) 259, 521
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 2 cut(s) 146, 513
PstNI CAGNNNCTG 1 cut(s) 415
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 123, 165
SatI GCNGC 2 cut(s) 413, 416
Sau3AI GATC 2 cut(s) 201, 226
Sau96I GGNCC 2 cut(s) 146, 513
ScrFI CCNGG 3 cut(s) 89, 261, 523
SetI ASST 6 cut(s) 10, 214, 286, 352, 357, 417
SfaNI GCATC 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 118, 321
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 2 cut(s) 136, 169
SspMI CTAG 4 cut(s) 143, 150, 234, 296
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 3 cut(s) 87, 259, 521
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 497
TaiI ACGT 2 cut(s) 214, 352
TasI AATT 2 cut(s) 118, 321
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 123, 165
Tru9I TTAA 2 cut(s) 123, 165
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 2 cut(s) 412, 415
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 4 cut(s) 48, 278, 282, 521
Van91I CCANNNNNTGG 1 cut(s) 470
Vha464I CTTAAG 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmaJI CCTAGG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 4 cut(s) 143, 150, 234, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.