Rh5BG513400

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007994
Physical Location & Seq
Reverse (-)
81431107 .. 81436740
5634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG513400.1

Sequence Viewer

Length: 480 bp
ATGTCAAGTTTATTACAGCCCTCATCAACACAAAATTGGCTTAGTCCTCAAAGTAGCTCATCTGGTCTAATTGTTAAGCGGACAATCAGAGTGGATATTCCCGTTGACAAATATCCTAATTATAACTTTGTTGGGCGGTTGCTAGGTCCTAGGGGAAACTCTCTTAAGCGAGTGGAAGCAACTACTGAGTGCCGTGTTCTGATCAGGGGCCGTGGTAGCATAAAGGATCCAGCCAGGGAAGATATGATGAGAGGTAAACCGGGGTATGAACATTTGAATGAACCTCTTCATATACTAGTGGAGGCAGAACTACCAGTTGAAATTGTTGATGCTCGTTTATTACAAGCGCGTGAAGTACTTGAAGATCTGCTCAGGCCTGTGGTATGTTTTTTCTTTCCTTGTGAAGAGAATTTTTCTTTTTTCTCTAGTAAATTTTGTTCATTCTGCCTGCAAGCCTCACTATCGACTATCTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 STAR_dimer

Protein Analysis

159

Amino Acids

17.93

Weight (kDa)

7.63

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH-I_KHDC4-BBP PF22675 37 - 124 7.9e-20 KHDC4/BBP-like, KH-domain type I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 123
AccII CGCG 1 cut(s) 349
AciI CCGC 2 cut(s) 79, 136
AclWI GGATC 2 cut(s) 221, 234
AcsI RAATTY 2 cut(s) 409, 431
AfaI GTAC 1 cut(s) 357
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 3 cut(s) 277, 320, 362
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 1 cut(s) 233
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
AlwI GGATC 2 cut(s) 221, 234
AoxI GGCC 2 cut(s) 208, 374
ApoI RAATTY 2 cut(s) 409, 431
Asp700I GAANNNNTTC 1 cut(s) 285
AspA2I CCTAGG 1 cut(s) 149
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 2 cut(s) 146, 208
AsuC2I CCSGG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 146
AvrII CCTAGG 1 cut(s) 149
BamHI GGATCC 1 cut(s) 226
BceAI ACGGC 2 cut(s) 177, 195
BciT130I CCWGG 1 cut(s) 235
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 1 cut(s) 261
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 4 cut(s) 143, 150, 296, 426
BfrI CTTAAG 1 cut(s) 164
BglII AGATCT 1 cut(s) 364
BlnI CCTAGG 1 cut(s) 149
BmcAI AGTACT 1 cut(s) 357
Bme1390I CCNGG 2 cut(s) 235, 261
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 146, 208
BmiI GGNNCC 2 cut(s) 209, 228
BmrFI CCNGG 2 cut(s) 235, 261
BmsI GCATC 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 261
BsaJI CCNNGG 4 cut(s) 149, 211, 234, 260
Bse1I ACTGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 4 cut(s) 149, 211, 234, 260
BseMII CTCAG 2 cut(s) 177, 385
BseNI ACTGG 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 2 cut(s) 210, 376
BsiSI CCGG 1 cut(s) 260
BsnI GGCC 2 cut(s) 210, 376
Bsp143I GATC 3 cut(s) 201, 226, 364
BspACI CCGC 2 cut(s) 79, 136
BspANI GGCC 2 cut(s) 210, 376
BspCNI CTCAG 2 cut(s) 178, 384
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 209, 228
BspPI GGATC 2 cut(s) 221, 234
BspTI CTTAAG 1 cut(s) 164
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 4 cut(s) 149, 211, 234, 260
BssMI GATC 3 cut(s) 201, 226, 364
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 291, 399
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 449, 453
BstDEI CTNAG 3 cut(s) 41, 186, 371
BstDSI CCRYGG 1 cut(s) 211
BstFNI CGCG 1 cut(s) 349
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 3 cut(s) 204, 229, 367
BstMBI GATC 3 cut(s) 201, 226, 364
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 233, 259
BstUI CGCG 1 cut(s) 349
BstX2I RGATCY 2 cut(s) 226, 364
BstYI RGATCY 2 cut(s) 226, 364
BsuRI GGCC 2 cut(s) 210, 376
BtgI CCRYGG 1 cut(s) 211
Cac8I GCNNGC 2 cut(s) 449, 453
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 146, 208
Csp6I GTAC 1 cut(s) 356
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviAII CATG 1 cut(s) 477
CviJI RGCY 7 cut(s) 19, 40, 57, 210, 233, 376, 455
CviKI_1 RGCY 7 cut(s) 19, 40, 57, 210, 233, 376, 455
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 3 cut(s) 41, 186, 371
DpnI GATC 3 cut(s) 203, 228, 366
DpnII GATC 3 cut(s) 201, 226, 364
Eam1104I CTCTTC 2 cut(s) 291, 399
EarI CTCTTC 2 cut(s) 291, 399
Eco130I CCWWGG 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 1 cut(s) 480
FaiI YATR 8 cut(s) 123, 221, 245, 267, 291, 293, 385, 478
FatI CATG 1 cut(s) 476
FbaI TGATCA 1 cut(s) 201
FspBI CTAG 4 cut(s) 143, 150, 296, 426
GlaI GCGC 1 cut(s) 348
HaeIII GGCC 2 cut(s) 210, 376
HapII CCGG 1 cut(s) 260
HhaI GCGC 1 cut(s) 349
Hin1II CATG 1 cut(s) 480
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HpaII CCGG 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 106, 257
Hpy188I TCNGA 2 cut(s) 89, 201
Hpy8I GTNNAC 2 cut(s) 106, 257
HpyCH4V TGCA 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 3 cut(s) 41, 186, 371
Hsp92II CATG 1 cut(s) 480
HspAI GCGC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 3 cut(s) 201, 226, 364
LweI GCATC 1 cut(s) 319
MaeI CTAG 4 cut(s) 143, 150, 296, 426
MalI GATC 3 cut(s) 203, 228, 366
MboI GATC 3 cut(s) 201, 226, 364
MboII GAAGA 4 cut(s) 251, 278, 374, 416
MflI RGATCY 2 cut(s) 226, 364
MluCI AATT 6 cut(s) 34, 69, 118, 321, 409, 431
MnlI CCTC 6 cut(s) 31, 57, 245, 294, 295, 466
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 2 cut(s) 75, 165
MslI CAYNNNNRTG 1 cut(s) 276
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 2 cut(s) 235, 261
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 261
NdeII GATC 3 cut(s) 201, 226, 364
NlaIII CATG 1 cut(s) 480
NlaIV GGNNCC 2 cut(s) 209, 228
PceI AGGCCT 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 285
PpuMI RGGWCCY 1 cut(s) 146
PsiI TTATAA 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 2 cut(s) 209, 228
PspPI GGNCC 2 cut(s) 146, 208
PspPPI RGGWCCY 1 cut(s) 146
PsrI GAACNNNNNNTAC 2 cut(s) 421, 453
PsuI RGATCY 2 cut(s) 226, 364
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 2 cut(s) 75, 165
Sau3AI GATC 3 cut(s) 201, 226, 364
Sau96I GGNCC 2 cut(s) 146, 208
ScaI AGTACT 1 cut(s) 357
ScrFI CCNGG 2 cut(s) 235, 261
SetI ASST 4 cut(s) 59, 148, 256, 286
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 6 cut(s) 34, 69, 118, 321, 409, 431
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 2 cut(s) 79, 136
SspMI CTAG 4 cut(s) 143, 150, 296, 426
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 2 cut(s) 233, 259
StyI CCWWGG 1 cut(s) 149
TaqI TCGA 1 cut(s) 464
TasI AATT 6 cut(s) 34, 69, 118, 321, 409, 431
TatI WGTACW 1 cut(s) 355
Tru1I TTAA 2 cut(s) 75, 165
Tru9I TTAA 2 cut(s) 75, 165
TspDTI ATGAA 4 cut(s) 278, 282, 294, 429
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 2 cut(s) 409, 431
XmaJI CCTAGG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 4 cut(s) 143, 150, 296, 426
ZrmI AGTACT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.