AT5G56190

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003717
Physical Location & Seq
Forward (+)
22741965 .. 22745191
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56190.2

Sequence Viewer

Length: 1326 bp
ATGGCTGACTTTGATGAATATATGGCTGATGAGTATGACATGGATGATCTCGAGGATGATATGAATGCCGCGTTTGATGGAAGAGATATCGATGCTTCTGATTCCGAAGTCGAAGATTTCGATCAATTGAACAAGGCTGCAGATACTTCTGCTGCTCAAGCACGGAATGGGAAGGATATTCAGGGTATACCGTGGGATAGTCTTACTCTTACTAGAAAAGATTACAGAAAAACTAGGCTAGAACAATATAAAAACTATGAAAATATACCCAATTCTGGTGATGAATCTGCCAAGAAATGCATGAATACTGAGAAAGGCAGCTCTTTCTATGAGTTCAGAAGGAATTCTAGATCCGTGAGATCAACGATTCTCCATTTTCAGTTGAGGAATTTGGTCTGGGCTACCTCAAAACACGATGTTTATTTATTGTCCAACTACTCCATCAGCCACTGGTCTTCTTTGACAGGTTGTAGGAGTGAAATTCTTAACGTTAAAGGTCATGTGGCTCCGTCTGAGAAACATCCCGAGAGTTTGTTGGAAGGATTTACAGAGACTCAAGTTAGCACTCTTGCAGTAAAAGAAAGGCTACTAGTTGCTGGTGGATTCCAAGGAGAACTCATTTGCAAGCATCTCGATAGACCTGGTGTGAGCTTCTGCTCACGCACAACTTACACCGAAAACGCTATCACCAATGCAGTAGATATATATAGAAACTCCAGCGGCGCACTGCATTTCATGGCCTCAAATAATGACTGTGGAGTCAGAGACTTTGACATGGAGAGATATAAGCTTGTCCAACTCTTCCGTTATCTATGGCCCGTCAATCATAGTTCCCTAAGTCCTGATGGAAAACTAGTAGCAGTCGTTGGAGATGACCCAGATGGTCTACTGGTAGACACAAGTAACGGACAGACGATCGGGACGCTAAAAGGTCACTTAGATTATTCATTTGCATCAGCTTGGCATCCAAACGGTGTAACATTTGCCACAGGAAACCAAGACAAGACCTGTCGTATTTGGGACACAAGGAAACTCTCGGAATCAGTCGCTGTCCTAAAAGGTAACCTTGGAGCCGTACGGTCCATTAGGTTCACATCTGACGGACGGTACGTTGCCATGGCCGAGCCAGCAGATTTCGTTCATATCTATGACACAAAATCAGGATACAAAAAGGAGCAAGAGATTGATTTCTTTGGAGAGATTTCTGGGATATCGTTTAGCCCAGATACTGAATCTCTCTTCATCGGTGTTTGGGACAGGACTTATGGAAGTCTCTTGGAGTATGGTCGAACCAGAGACTACACTTATCTCGATTCATTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

441

Amino Acids

49.68

Weight (kDa)

5.04

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_2nd PF25168 233 - 384 1.5e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 238 - 389 6.5e-07 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 246 - 353 8.5e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 249 - 393 1.5e-15 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 251 - 396 6.8e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 251 - 427 6.2e-18 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 252 - 419 5.2e-20 THOC3 beta-propeller domain
WD40_Gbeta PF25391 278 - 398 1.9e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 280 - 426 8.8e-11 WDR3 first beta-propeller domain
Beta-prop_IFT122_1st PF23381 280 - 385 6.7e-06 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 283 - 419 4.4e-10 CDC20/Fizzy WD40 domain
WDR55 PF24796 284 - 394 6.7e-07 WDR55
WD40 PF00400 309 - 347 1.1e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 326 - 426 2.1e-09 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 758
AccI GTMKAC 3 cut(s) 187, 886, 894
AccII CGCG 1 cut(s) 71
AciI CCGC 2 cut(s) 69, 720
AclI AACGTT 1 cut(s) 489
AclWI GGATC 1 cut(s) 345
AcoI YGGCCR 1 cut(s) 1119
AcsI RAATTY 3 cut(s) 343, 388, 480
AfaI GTAC 2 cut(s) 1077, 1109
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 1 cut(s) 130
AhlI ACTAGT 2 cut(s) 589, 853
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 4 cut(s) 321, 651, 790, 959
AluI AGCT 4 cut(s) 321, 651, 790, 959
Alw26I GTCTC 4 cut(s) 545, 759, 1277, 1290
AlwI GGATC 1 cut(s) 345
AlwNI CAGNNNCTG 3 cut(s) 450, 1049, 1229
Ama87I CYCGRG 2 cut(s) 50, 524
AoxI GGCC 3 cut(s) 738, 815, 1119
ApeKI GCWGC 3 cut(s) 137, 152, 318
ApoI RAATTY 3 cut(s) 343, 388, 480
Asp700I GAANNNNTTC 1 cut(s) 343
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 816, 1080
AsuHPI GGTGA 2 cut(s) 290, 679
AvaI CYCGRG 2 cut(s) 50, 524
AvaII GGWCC 1 cut(s) 1080
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 124, 139, 330
BccI CCATC 4 cut(s) 71, 449, 839, 875
BceAI ACGGC 1 cut(s) 1058
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BciT130I CCWGG 1 cut(s) 642
BciVI GTATCC 1 cut(s) 1157
BcoDI GTCTC 4 cut(s) 545, 759, 1277, 1290
BcuI ACTAGT 2 cut(s) 589, 853
BfaI CTAG 6 cut(s) 213, 234, 239, 348, 590, 854
BfmI CTRYAG 1 cut(s) 138
BfuI GTATCC 1 cut(s) 1157
BisI GCNGC 5 cut(s) 69, 138, 153, 319, 721
BlsI GCNGC 5 cut(s) 70, 139, 154, 320, 722
Bme1390I CCNGG 1 cut(s) 642
Bme18I GGWCC 1 cut(s) 1080
BmeT110I CYCGRG 2 cut(s) 50, 524
BmgT120I GGNCC 2 cut(s) 816, 1080
BmiI GGNNCC 2 cut(s) 507, 1072
BmrFI CCNGG 1 cut(s) 642
BmsI GCATC 4 cut(s) 82, 637, 962, 973
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 700
BpuEI CTTGAG 2 cut(s) 141, 540
Bsa29I ATCGAT 1 cut(s) 90
BsaBI GATNNNNATC 1 cut(s) 120
BsaJI CCNNGG 4 cut(s) 191, 607, 1066, 1116
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 455, 894
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 1 cut(s) 642
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 4 cut(s) 191, 607, 1066, 1116
BseGI GGATG 4 cut(s) 49, 61, 520, 964
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 300, 504
BseNI ACTGG 2 cut(s) 455, 894
BseXI GCAGC 3 cut(s) 124, 139, 330
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 3 cut(s) 740, 817, 1121
BshVI ATCGAT 1 cut(s) 90
BsiEI CGRYCG 1 cut(s) 918
BsiHKCI CYCGRG 2 cut(s) 50, 524
BsiWI CGTACG 1 cut(s) 1075
BslFI GGGAC 3 cut(s) 934, 1034, 1268
BslI CCNNNNNNNGG 1 cut(s) 275
BsmAI GTCTC 4 cut(s) 545, 759, 1277, 1290
BsmFI GGGAC 3 cut(s) 934, 1034, 1268
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 3 cut(s) 740, 817, 1121
BsoBI CYCGRG 2 cut(s) 50, 524
Bsp143I GATC 5 cut(s) 46, 121, 350, 359, 915
Bsp19I CCATGG 1 cut(s) 1116
BspACI CCGC 2 cut(s) 69, 720
BspANI GGCC 3 cut(s) 740, 817, 1121
BspCNI CTCAG 2 cut(s) 301, 505
BspDI ATCGAT 1 cut(s) 90
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 507, 1072
BspMAI CTGCAG 1 cut(s) 142
BspPI GGATC 1 cut(s) 345
BsrI ACTGG 2 cut(s) 455, 894
BssECI CCNNGG 4 cut(s) 191, 607, 1066, 1116
BssMI GATC 5 cut(s) 46, 121, 350, 359, 915
BssNAI GTATAC 1 cut(s) 188
BssT1I CCWWGG 3 cut(s) 607, 1066, 1116
Bst1107I GTATAC 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 5 cut(s) 192, 755, 974, 1080, 1107
Bst6I CTCTTC 3 cut(s) 76, 806, 1244
BstC8I GCNNGC 2 cut(s) 626, 1128
BstDEI CTNAG 4 cut(s) 309, 513, 836, 937
BstDSI CCRYGG 2 cut(s) 191, 1116
BstEII GGTNACC 1 cut(s) 1061
BstF5I GGATG 4 cut(s) 49, 61, 520, 964
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 5 cut(s) 49, 124, 353, 362, 918
BstMAI GTCTC 4 cut(s) 545, 759, 1277, 1290
BstMBI GATC 5 cut(s) 46, 121, 350, 359, 915
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 2 cut(s) 158, 1127
BstNI CCWGG 1 cut(s) 642
BstPI GGTNACC 1 cut(s) 1061
BstSCI CCNGG 1 cut(s) 640
BstSFI CTRYAG 1 cut(s) 138
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 124, 139, 330
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BstZ17I GTATAC 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 90
BsuI GTATCC 1 cut(s) 1157
BsuRI GGCC 3 cut(s) 740, 817, 1121
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 2 cut(s) 191, 1116
BtsCI GGATG 4 cut(s) 49, 61, 520, 964
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 2 cut(s) 448, 725
Cac8I GCNNGC 2 cut(s) 626, 1128
CaiI CAGNNNCTG 3 cut(s) 450, 1049, 1229
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 2 cut(s) 816, 1080
ClaI ATCGAT 1 cut(s) 90
CseI GACGC 1 cut(s) 931
CsiI ACCWGGT 1 cut(s) 640
Csp6I GTAC 2 cut(s) 1076, 1108
CviAII CATG 6 cut(s) 40, 301, 500, 736, 775, 1117
CviQI GTAC 2 cut(s) 1076, 1108
DdeI CTNAG 4 cut(s) 309, 513, 836, 937
DpnI GATC 5 cut(s) 48, 123, 352, 361, 917
DpnII GATC 5 cut(s) 46, 121, 350, 359, 915
DrdI GACNNNNNNGTC 1 cut(s) 758
DseDI GACNNNNNNGTC 1 cut(s) 758
EaeI YGGCCR 1 cut(s) 1119
Eam1104I CTCTTC 3 cut(s) 76, 806, 1244
EarI CTCTTC 3 cut(s) 76, 806, 1244
Eco130I CCWWGG 3 cut(s) 607, 1066, 1116
Eco32I GATATC 2 cut(s) 88, 1212
Eco47I GGWCC 1 cut(s) 1080
Eco88I CYCGRG 2 cut(s) 50, 524
Eco91I GGTNACC 1 cut(s) 1061
EcoO65I GGTNACC 1 cut(s) 1061
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 640
EcoRV GATATC 2 cut(s) 88, 1212
EcoT14I CCWWGG 3 cut(s) 607, 1066, 1116
EcoT22I ATGCAT 1 cut(s) 302
ErhI CCWWGG 3 cut(s) 607, 1066, 1116
FaeI CATG 6 cut(s) 43, 304, 503, 739, 778, 1120
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 3 cut(s) 934, 1034, 1268
FatI CATG 6 cut(s) 39, 300, 499, 735, 774, 1116
FblI GTMKAC 3 cut(s) 187, 886, 894
Fnu4HI GCNGC 5 cut(s) 69, 138, 153, 319, 721
FokI GGATG 4 cut(s) 56, 68, 507, 951
Fsp4HI GCNGC 5 cut(s) 69, 138, 153, 319, 721
FspBI CTAG 6 cut(s) 213, 234, 239, 348, 590, 854
GlaI GCGC 1 cut(s) 724
GluI GCNGC 5 cut(s) 69, 138, 153, 319, 721
GsuI CTGGAG 1 cut(s) 700
HaeIII GGCC 3 cut(s) 740, 817, 1121
HgaI GACGC 1 cut(s) 931
HhaI GCGC 1 cut(s) 725
Hin1II CATG 6 cut(s) 43, 304, 503, 739, 778, 1120
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 9 cut(s) 101, 284, 367, 553, 603, 759, 1040, 1232, 1313
HphI GGTGA 2 cut(s) 290, 679
Hpy166II GTNNAC 4 cut(s) 188, 887, 895, 1092
Hpy188I TCNGA 7 cut(s) 100, 106, 338, 514, 764, 1039, 1099
Hpy188III TCNNGA 8 cut(s) 50, 348, 524, 632, 842, 919, 1161, 1310
Hpy8I GTNNAC 4 cut(s) 188, 887, 895, 1092
HpyAV CCTTC 3 cut(s) 166, 333, 533
HpyCH4III ACNGT 5 cut(s) 192, 755, 974, 1080, 1107
HpyCH4IV ACGT 2 cut(s) 489, 1110
HpyCH4V TGCA 7 cut(s) 140, 300, 572, 624, 695, 730, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 1127
HpyF3I CTNAG 4 cut(s) 309, 513, 836, 937
HpySE526I ACGT 2 cut(s) 489, 1110
Hsp92II CATG 6 cut(s) 43, 304, 503, 739, 778, 1120
HspAI GCGC 1 cut(s) 723
Kzo9I GATC 5 cut(s) 46, 121, 350, 359, 915
LmnI GCTCC 3 cut(s) 511, 1070, 1174
Lsp1109I GCAGC 3 cut(s) 124, 139, 330
LweI GCATC 4 cut(s) 82, 637, 962, 973
MabI ACCWGGT 1 cut(s) 640
MaeI CTAG 6 cut(s) 213, 234, 239, 348, 590, 854
MaeII ACGT 2 cut(s) 489, 1110
MaeIII GTNAC 4 cut(s) 902, 932, 976, 1061
MalI GATC 5 cut(s) 48, 123, 352, 361, 917
MboI GATC 5 cut(s) 46, 121, 350, 359, 915
MboII GAAGA 5 cut(s) 93, 125, 447, 793, 1231
MfeI CAATTG 1 cut(s) 125
MflI RGATCY 1 cut(s) 350
MluCI AATT 5 cut(s) 125, 271, 343, 388, 480
MlyI GAGTC 2 cut(s) 547, 768
MmeI TCCRAC 4 cut(s) 456, 516, 820, 847
MnlI CCTC 4 cut(s) 46, 378, 415, 751
Mph1103I ATGCAT 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 343
MseI TTAA 2 cut(s) 486, 492
MslI CAYNNNNRTG 1 cut(s) 1146
MspA1I CMGCKG 1 cut(s) 720
MspR9I CCNGG 1 cut(s) 642
MunI CAATTG 1 cut(s) 125
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 158, 1127
NcoI CCATGG 1 cut(s) 1116
NdeII GATC 5 cut(s) 46, 121, 350, 359, 915
NlaIII CATG 6 cut(s) 43, 304, 503, 739, 778, 1120
NlaIV GGNNCC 2 cut(s) 507, 1072
NmeAIII GCCGAG 1 cut(s) 1147
NmuCI GTSAC 1 cut(s) 932
NsiI ATGCAT 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 50
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 1107
PctI GAATGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 343
PfeI GAWTC 7 cut(s) 101, 284, 367, 603, 1040, 1232, 1313
Pfl23II CGTACG 1 cut(s) 1075
PkrI GCNGC 5 cut(s) 70, 139, 154, 320, 722
Ple19I CGATCG 1 cut(s) 918
PleI GAGTC 2 cut(s) 547, 767
PpsI GAGTC 2 cut(s) 547, 767
Psp1406I AACGTT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 640
PspEI GGTNACC 1 cut(s) 1061
PspGI CCWGG 1 cut(s) 640
PspLI CGTACG 1 cut(s) 1075
PspN4I GGNNCC 2 cut(s) 507, 1072
PspPI GGNCC 2 cut(s) 816, 1080
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 3 cut(s) 450, 1049, 1229
PsuI RGATCY 1 cut(s) 350
PvuI CGATCG 1 cut(s) 918
RsaI GTAC 2 cut(s) 1077, 1109
RsaNI GTAC 2 cut(s) 1076, 1108
RseI CAYNNNNRTG 1 cut(s) 1146
SaqAI TTAA 2 cut(s) 486, 492
SatI GCNGC 5 cut(s) 69, 138, 153, 319, 721
Sau3AI GATC 5 cut(s) 46, 121, 350, 359, 915
Sau96I GGNCC 2 cut(s) 816, 1080
SchI GAGTC 2 cut(s) 547, 768
ScrFI CCNGG 1 cut(s) 642
SexAI ACCWGGT 1 cut(s) 640
SfaNI GCATC 4 cut(s) 82, 637, 962, 973
SfcI CTRYAG 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 50
SinI GGWCC 1 cut(s) 1080
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 1146
SmlI CTYRAG 3 cut(s) 50, 156, 555
SmoI CTYRAG 3 cut(s) 50, 156, 555
SpeI ACTAGT 2 cut(s) 589, 853
Sse9I AATT 5 cut(s) 125, 271, 343, 388, 480
SsiI CCGC 2 cut(s) 69, 720
SspMI CTAG 6 cut(s) 213, 234, 239, 348, 590, 854
StyD4I CCNGG 1 cut(s) 640
StyI CCWWGG 3 cut(s) 607, 1066, 1116
TaaI ACNGT 5 cut(s) 192, 755, 974, 1080, 1107
TaiI ACGT 2 cut(s) 492, 1113
TaqI TCGA 7 cut(s) 51, 90, 111, 120, 633, 1288, 1311
TasI AATT 5 cut(s) 125, 271, 343, 388, 480
TauI GCSGC 2 cut(s) 71, 723
TfiI GAWTC 7 cut(s) 101, 284, 367, 603, 1040, 1232, 1313
Tru1I TTAA 2 cut(s) 486, 492
Tru9I TTAA 2 cut(s) 486, 492
TscAI CASTG 2 cut(s) 455, 732
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 3 cut(s) 137, 152, 318
Tsp45I GTSAC 1 cut(s) 932
TspGWI ACGGA 6 cut(s) 178, 343, 498, 794, 921, 1116
TspRI CASTG 2 cut(s) 455, 732
VpaK11BI GGWCC 1 cut(s) 1080
XapI RAATTY 3 cut(s) 343, 388, 480
XbaI TCTAGA 1 cut(s) 347
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 3 cut(s) 187, 886, 894
XmnI GAANNNNTTC 1 cut(s) 343
XspI CTAG 6 cut(s) 213, 234, 239, 348, 590, 854
Zsp2I ATGCAT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.