Rroxscaffold_5G00355270

WD repeat-containing protein C2A9.03-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003717
Physical Location & Seq
Reverse (-)
33907259 .. 33913140
5882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355270.1

Sequence Viewer

Length: 1326 bp
ATGGCTTTTGAGCAGCCTCATCAGTTTGAATATATGGCGGATGAGGGTGACATGTCTGATTTCGTTGAGGACGCAGATGCAGATGCTGATGAAGAAAATCATGGTAGAGATATGGTTCTTGACGAATACGACATGGTGTCGGATACATCTTCTGCCCAAGCAAGAAAAGGAAAGGATATTCAGGGTATTCCATGGGATAGGTTAAACATAACGAGGGAGAAGTACAGATTGACGAGGCTTGAACAGTACAAGAATTATGAGAATATCCCTTCATCTGGGGAAGCTGTGGACAAGGAGTGTCAACTAACGCAGAAGGGTGGCAACTACTATGAATTCTTCCACAACACAAGACTGGTTAAACCTACAATCCTTCATTTTCAGCTTAGAAATCTGATTTGGGCTACTTCGAAACATGACGTCTATCTCATGTCCAATTATTCAGTTATGCATTGGTCTTCTCTATCTGGCAATTTGTCTGAGGTCCTCAATTTTTCAGGACATGTTGCACCAACTGAGAAACATGCAGGAAGTTTGTTGGAGGGTTTTATGCAGACTCAGATAAGCACCCTCGCAGTCAAGGACAATTTTCTTGTTGCTGGAGGTTTCCAAGGAGAGCTTACTTGCAAGCGTCTTGATAAAGAAGGAGTGAGTTTTTGTACGCGAACCACATACGATGACAATGCGATCACAAATGCTGTTGAAATATATGATAGCCTGGGGGGTGGAATGCATTTCATGGCATCCAATAATGATTGTGGTATAAGAGAATATGACATGGAGAGATTTCAGCTTCTGAATCACTTTCGATTCCCCTGGCCAGTGAATCATACATCAATAAGCCCAGATCGTAAGGTTGTTGCCGTAGTTGGAGATCACGTGGATGGATTGCTTATGGATTCTCAGAATGGAAAGACTATTGCTAAGGTTGTTGGTCATCTAGATTACTCCTTTGCTTCTGCATGGCATCCTGATGGCTGCATATTTGCCACCGGAAATCAGGACAAGACTTCCCGAGTTTGGGATATAAGAAATCTTTCATCACCGCTTGCAGTTCTGAAGAATAACTTGGGTGCTACTCGGTCTATTCGGTTTTCATCAGATGGTCAGTTTATGATGGTTGCAGAACCTGCAGATTTTGTGCATATATATAGTACAAGGGCAGATTACAAAAAACGGCAAGAGATTGATTTCTTTGGGGAGATTTCGGGGGTATCAGCAAGTCCAGATGACGAGTCCTTGTACATAGGAATCTGGGACCGAACATACGCAAGCTTGTTGCAATATAACAGAAGGCATACATATGGATATCTTGATTCCTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

441

Amino Acids

50.16

Weight (kDa)

5.14

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 242 - 412 1.2e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 243 - 389 2.6e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 246 - 387 8.1e-11 WDR5 beta-propeller domain
WD40_Prp19 PF24814 273 - 397 7.5e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 318 - 395 2.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 420
Acc36I ACCTGC 1 cut(s) 1135
AccII CGCG 1 cut(s) 661
AciI CCGC 2 cut(s) 38, 1043
AcoI YGGCCR 1 cut(s) 815
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 1076
AcvI CACGTG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 5 cut(s) 224, 248, 658, 1153, 1241
AfiI CCNNNNNNNGG 3 cut(s) 275, 1017, 1018
AflIII ACRYGT 2 cut(s) 51, 499
AgsI TTSAA 3 cut(s) 29, 242, 701
AhdI GACNNNNNGTC 1 cut(s) 136
AjnI CCWGG 2 cut(s) 714, 812
AjuI GAANNNNNNNTTGG 4 cut(s) 379, 411, 1049, 1081
AluBI AGCT 5 cut(s) 284, 382, 616, 790, 1272
AluI AGCT 5 cut(s) 284, 382, 616, 790, 1272
AlwNI CAGNNNCTG 3 cut(s) 86, 793, 1127
Ama87I CYCGRG 1 cut(s) 1011
AoxI GGCC 1 cut(s) 815
ApeKI GCWGC 2 cut(s) 13, 975
ApoI RAATTY 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 1033
AspS9I GGNCC 2 cut(s) 481, 1255
AsuHPI GGTGA 2 cut(s) 59, 1032
AsuII TTCGAA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 1011
AvaII GGWCC 2 cut(s) 481, 1255
BalI TGGCCA 1 cut(s) 817
BbrPI CACGTG 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 25, 962
BccI CCATC 4 cut(s) 875, 965, 1094, 1108
BceAI ACGGC 2 cut(s) 845, 1190
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 2 cut(s) 716, 814
BciVI GTATCC 1 cut(s) 136
BfaI CTAG 1 cut(s) 938
BfmI CTRYAG 1 cut(s) 1128
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 1 cut(s) 136
BisI GCNGC 2 cut(s) 14, 976
BlsI GCNGC 2 cut(s) 15, 977
Bme1390I CCNGG 2 cut(s) 716, 814
Bme18I GGWCC 2 cut(s) 481, 1255
BmeRI GACNNNNNGTC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 1011
BmgT120I GGNCC 2 cut(s) 481, 1255
BmiI GGNNCC 1 cut(s) 1256
BmrFI CCNGG 2 cut(s) 716, 814
BmsI GCATC 4 cut(s) 67, 73, 749, 973
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 921
Bpu14I TTCGAA 1 cut(s) 407
BsaAI YACGTR 1 cut(s) 877
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 4 cut(s) 191, 607, 715, 812
BsaWI WCCGGW 1 cut(s) 989
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 1017, 1018
Bse1I ACTGG 2 cut(s) 357, 818
BseBI CCWGG 2 cut(s) 716, 814
BseDI CCNNGG 4 cut(s) 191, 607, 715, 812
BseGI GGATG 4 cut(s) 46, 740, 886, 964
BseLI CCNNNNNNNGG 3 cut(s) 275, 1017, 1018
BseMII CTCAG 4 cut(s) 468, 504, 569, 914
BseNI ACTGG 2 cut(s) 357, 818
BseXI GCAGC 2 cut(s) 25, 962
Bsh1236I CGCG 1 cut(s) 661
BshFI GGCC 1 cut(s) 817
BsiHKCI CYCGRG 1 cut(s) 1011
BsiSI CCGG 1 cut(s) 990
BslFI GGGAC 1 cut(s) 1268
BslI CCNNNNNNNGG 3 cut(s) 275, 1017, 1018
BsmFI GGGAC 1 cut(s) 1268
BsmI GAATGC 1 cut(s) 732
BsnI GGCC 1 cut(s) 817
BsoBI CYCGRG 1 cut(s) 1011
Bsp119I TTCGAA 1 cut(s) 407
Bsp1407I TGTACA 1 cut(s) 1239
Bsp143I GATC 3 cut(s) 684, 844, 871
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 2 cut(s) 38, 1043
BspANI GGCC 1 cut(s) 817
BspCNI CTCAG 4 cut(s) 469, 505, 568, 913
BspFNI CGCG 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 1256
BspMAI CTGCAG 1 cut(s) 1132
BspMI ACCTGC 1 cut(s) 1135
BspT104I TTCGAA 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 1239
BsrI ACTGG 2 cut(s) 357, 818
BssECI CCNNGG 4 cut(s) 191, 607, 715, 812
BssMI GATC 3 cut(s) 684, 844, 871
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 2 cut(s) 191, 607
Bst2UI CCWGG 2 cut(s) 716, 814
Bst4CI ACNGT 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 417
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstAUI TGTACA 1 cut(s) 1239
BstBAI YACGTR 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 3 cut(s) 626, 1047, 1270
BstDEI CTNAG 6 cut(s) 383, 477, 513, 555, 900, 921
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 4 cut(s) 46, 740, 886, 964
BstFNI CGCG 1 cut(s) 661
BstKTI GATC 3 cut(s) 687, 847, 874
BstMBI GATC 3 cut(s) 684, 844, 871
BstMWI GCNNNNNNNGC 1 cut(s) 1127
BstNI CCWGG 2 cut(s) 716, 814
BstNSI RCATGY 3 cut(s) 55, 503, 524
BstSCI CCNGG 2 cut(s) 714, 812
BstSFI CTRYAG 1 cut(s) 1128
BstUI CGCG 1 cut(s) 661
BstV1I GCAGC 2 cut(s) 25, 962
BstV2I GAAGAC 1 cut(s) 447
BsuI GTATCC 1 cut(s) 136
BsuRI GGCC 1 cut(s) 817
BtgI CCRYGG 1 cut(s) 191
BtsCI GGATG 4 cut(s) 46, 740, 886, 964
BtsIMutI CAGTG 1 cut(s) 825
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 3 cut(s) 626, 1047, 1270
CaiI CAGNNNCTG 3 cut(s) 86, 793, 1127
Cfr13I GGNCC 2 cut(s) 481, 1255
CseI GACGC 2 cut(s) 80, 617
Csp6I GTAC 5 cut(s) 223, 247, 657, 1152, 1240
CviQI GTAC 5 cut(s) 223, 247, 657, 1152, 1240
DdeI CTNAG 6 cut(s) 383, 477, 513, 555, 900, 921
DpnI GATC 3 cut(s) 686, 846, 873
DpnII GATC 3 cut(s) 684, 844, 871
DriI GACNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 815
Eam1105I GACNNNNNGTC 1 cut(s) 136
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 191, 607
Eco32I GATATC 1 cut(s) 1307
Eco47I GGWCC 2 cut(s) 481, 1255
Eco57I CTGAAG 1 cut(s) 1076
Eco72I CACGTG 1 cut(s) 877
Eco88I CYCGRG 1 cut(s) 1011
EcoO109I RGGNCCY 1 cut(s) 481
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 714, 812
EcoRV GATATC 1 cut(s) 1307
EcoT14I CCWWGG 2 cut(s) 191, 607
EcoT22I ATGCAT 2 cut(s) 450, 732
ErhI CCWWGG 2 cut(s) 191, 607
FalI AAGNNNNNCTT 4 cut(s) 600, 632, 1049, 1081
FaqI GGGAC 1 cut(s) 1268
FauNDI CATATG 1 cut(s) 1300
Fnu4HI GCNGC 2 cut(s) 14, 976
FokI GGATG 4 cut(s) 53, 727, 893, 951
Fsp4HI GCNGC 2 cut(s) 14, 976
FspBI CTAG 1 cut(s) 938
GluI GCNGC 2 cut(s) 14, 976
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 1 cut(s) 817
HapII CCGG 1 cut(s) 990
HgaI GACGC 2 cut(s) 80, 617
Hin1I GRCGYC 1 cut(s) 417
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HindIII AAGCTT 1 cut(s) 1270
HinfI GANTC 8 cut(s) 553, 796, 807, 823, 896, 1232, 1248, 1313
HpaII CCGG 1 cut(s) 990
HphI GGTGA 2 cut(s) 59, 1032
Hpy166II GTNNAC 2 cut(s) 289, 302
Hpy188I TCNGA 9 cut(s) 58, 142, 393, 478, 558, 795, 903, 1056, 1099
Hpy188III TCNNGA 9 cut(s) 119, 495, 632, 938, 968, 998, 1011, 1223, 1310
Hpy8I GTNNAC 2 cut(s) 289, 302
HpyAV CCTTC 5 cut(s) 279, 307, 380, 635, 1284
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4IV ACGT 2 cut(s) 417, 876
HpyF10VI GCNNNNNNNGC 1 cut(s) 1127
HpyF3I CTNAG 6 cut(s) 383, 477, 513, 555, 900, 921
HpySE526I ACGT 2 cut(s) 417, 876
Hsp92I GRCGYC 1 cut(s) 417
Kzo9I GATC 3 cut(s) 684, 844, 871
Lsp1109I GCAGC 2 cut(s) 25, 962
LweI GCATC 4 cut(s) 67, 73, 749, 973
MaeI CTAG 1 cut(s) 938
MaeII ACGT 2 cut(s) 417, 876
MaeIII GTNAC 1 cut(s) 47
MalI GATC 3 cut(s) 686, 846, 873
MboI GATC 3 cut(s) 684, 844, 871
MboII GAAGA 5 cut(s) 104, 141, 328, 447, 1069
MlsI TGGCCA 1 cut(s) 817
MluCI AATT 6 cut(s) 253, 332, 433, 469, 487, 583
MluNI TGGCCA 1 cut(s) 817
MlyI GAGTC 2 cut(s) 547, 1241
MmeI TCCRAC 3 cut(s) 120, 516, 847
Mox20I TGGCCA 1 cut(s) 817
Mph1103I ATGCAT 2 cut(s) 450, 732
MroXI GAANNNNTTC 1 cut(s) 1033
MscI TGGCCA 1 cut(s) 817
MseI TTAA 2 cut(s) 203, 357
MslI CAYNNNNRTG 3 cut(s) 879, 969, 1299
Msp20I TGGCCA 1 cut(s) 817
MspI CCGG 1 cut(s) 990
MspR9I CCNGG 2 cut(s) 716, 814
Mva1269I GAATGC 1 cut(s) 732
MvaI CCWGG 2 cut(s) 716, 814
MvnI CGCG 1 cut(s) 661
MwoI GCNNNNNNNGC 1 cut(s) 1127
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 1 cut(s) 1300
NdeII GATC 3 cut(s) 684, 844, 871
NlaIV GGNNCC 1 cut(s) 1256
NmuCI GTSAC 1 cut(s) 47
NsiI ATGCAT 2 cut(s) 450, 732
NspI RCATGY 3 cut(s) 55, 503, 524
NspV TTCGAA 1 cut(s) 407
PciI ACATGT 2 cut(s) 51, 499
PctI GAATGC 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 6 cut(s) 796, 807, 823, 896, 1248, 1313
PkrI GCNGC 2 cut(s) 15, 977
PleI GAGTC 2 cut(s) 547, 1240
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 2 cut(s) 547, 1240
Ppu21I YACGTR 1 cut(s) 877
PpuMI RGGWCCY 1 cut(s) 481
PscI ACATGT 2 cut(s) 51, 499
Psp5II RGGWCCY 1 cut(s) 481
Psp6I CCWGG 2 cut(s) 714, 812
PspCI CACGTG 1 cut(s) 877
PspGI CCWGG 2 cut(s) 714, 812
PspN4I GGNNCC 1 cut(s) 1256
PspPI GGNCC 2 cut(s) 481, 1255
PspPPI RGGWCCY 1 cut(s) 481
PstI CTGCAG 1 cut(s) 1132
PstNI CAGNNNCTG 3 cut(s) 86, 793, 1127
RsaI GTAC 5 cut(s) 224, 248, 658, 1153, 1241
RsaNI GTAC 5 cut(s) 223, 247, 657, 1152, 1240
RseI CAYNNNNRTG 3 cut(s) 879, 969, 1299
SaqAI TTAA 2 cut(s) 203, 357
SatI GCNGC 2 cut(s) 14, 976
Sau3AI GATC 3 cut(s) 684, 844, 871
Sau96I GGNCC 2 cut(s) 481, 1255
SchI GAGTC 2 cut(s) 547, 1241
ScrFI CCNGG 2 cut(s) 716, 814
SfaNI GCATC 4 cut(s) 67, 73, 749, 973
SfcI CTRYAG 1 cut(s) 1128
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 2 cut(s) 481, 1255
SmiMI CAYNNNNRTG 3 cut(s) 879, 969, 1299
Sse9I AATT 6 cut(s) 253, 332, 433, 469, 487, 583
SsiI CCGC 2 cut(s) 38, 1043
SspMI CTAG 1 cut(s) 938
StyD4I CCNGG 2 cut(s) 714, 812
StyI CCWWGG 2 cut(s) 191, 607
TaaI ACNGT 1 cut(s) 246
TaiI ACGT 2 cut(s) 420, 879
TaqI TCGA 2 cut(s) 407, 805
TaqII GACCGA 2 cut(s) 1068, 1272
TasI AATT 6 cut(s) 253, 332, 433, 469, 487, 583
TatI WGTACW 4 cut(s) 222, 246, 1151, 1239
TfiI GAWTC 6 cut(s) 796, 807, 823, 896, 1248, 1313
Tru1I TTAA 2 cut(s) 203, 357
Tru9I TTAA 2 cut(s) 203, 357
TscAI CASTG 1 cut(s) 825
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 2 cut(s) 13, 975
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 7 cut(s) 105, 261, 345, 362, 724, 1026, 1083
TspRI CASTG 1 cut(s) 825
VpaK11BI GGWCC 2 cut(s) 481, 1255
XapI RAATTY 1 cut(s) 332
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 3 cut(s) 55, 503, 524
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 1 cut(s) 938
ZraI GACGTC 1 cut(s) 418
Zsp2I ATGCAT 2 cut(s) 450, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.