MD04G1027500.v1.1

WD repeat-containing protein C2A9.03-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003717
Physical Location & Seq
Forward (+)
3144610 .. 3150174
5565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1027500.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGTGGATGAAGGGGACATGTCTGATTTTGTAGAGGATGTGGAGGAAGAAAATCAAGGTGGAGATATGGGTCTTGATGAATATGACATGCTAACCAAAGTGTCAGATACGTCATCTGCACAAGCGAGAAAGGGAAAGGACATTCAAGGTATTCCATGGGAAAGGTTGAACATAACAAGGGAAAAATACAGATTGACGAGGCTAGAACAGTACAGGAATTATGAGAACATCCCTTTATCTGGGGAAGCTGTGGACAAGGAGTGCAAACAAATGCAGAAAGGTGGAAGCTACTATGACTTCTTCCATAATACAAGACTGGTTAAACCTACAATCCTTCATTTCCAGCTGAGAAATTTGGTTTGGGCCACTTCAAAGCATGATGTCTACCTCATGTCCAACTTTTCAGTCATGCATTGGTCTTCTCTATCTGGGAATTTGTCTGAGGTTCTCAATTTTGCAGGACATGTTGCCCCAACAGAGAAACATGCAGGAAGTTTATTGGAGGGTTTTATGCAGACTCAGATAAGCACACTGGCAGTCAAGGACAATTTTCTTGTTGCTGGTGGCTTTCAAGGAGAGCTTACTTGTAAATCTCTTGATAAACAAGGAGTAAGTTTCTGTACCCGGACCACACATGATGACAATGCCATCACAAATGCTGTTGAAATATACGATAGATTGGGGGGTGGAATGCATTTCATGGCATCCAATAATGACTGCGGCATAAGAGAATATGACATGGAGAAGTTTCAGCTTTTGAATCACTTTCGTTTCCCTTGGCCAGTGAATCATACATCAATAAGCCCAGATCGTAGGCTTGTTGCCGTTGTTGGTGATCATGTAGATGCATTGCTCATGGATTCGCAGAATGGGAAGACTATTGCTAAGGTCGTTGGTCATCTGGATTACTCTTTTGCGTCAGCATGGCATCCTGATGGTCGCATATTTGCCACCGGAAACCAGGACAAGACTTCCCGAGTCTGGGACATAAGAAATCTGTCATTGCCAGTTGCTGTTCTGAAGAATAACTTGGGTGCTGCTAGGTCAATTCGCTTTTCATCTGATGGTCAGTTCATGGTGGTTGCCGAACCTGCGGATTTTGTGCATATATACAGCACAAAGGCAGATTACAAAGTGCGGCAAGAGATTGATTTCTTTGGGGAGATATCCGGTGTATCAATGAGTCCTGATGACGAGTCTCTGTATATAGGAATCTGGGACCGAACATACGCCAGCTTGCTACAGTATAACAGGAGGCATACGTACGGATATCTGGATTCCTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

430

Amino Acids

48.67

Weight (kDa)

5.45

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 182 - 374 2.2e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 230 - 401 1e-18 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 231 - 374 4.8e-07 WDHD1 first WD40 domain
WD40_Gbeta PF25391 232 - 367 2.3e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 233 - 373 1.5e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 234 - 408 1e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 260 - 378 2.9e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 262 - 408 2.3e-07 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 308 - 409 3.5e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1099
AccI GTMKAC 1 cut(s) 384
AciI CCGC 3 cut(s) 720, 1094, 1138
AcoI YGGCCR 1 cut(s) 779
AcsI RAATTY 2 cut(s) 352, 433
AcuI CTGAAG 1 cut(s) 1040
AfaI GTAC 3 cut(s) 212, 622, 1265
AfiI CCNNNNNNNGG 3 cut(s) 239, 981, 982
AflIII ACRYGT 2 cut(s) 18, 463
AgsI TTSAA 6 cut(s) 146, 169, 372, 572, 665, 760
AjnI CCWGG 1 cut(s) 960
AjuI GAANNNNNNNTTGG 4 cut(s) 343, 375, 1013, 1045
AluBI AGCT 6 cut(s) 248, 288, 346, 580, 754, 1236
AluI AGCT 6 cut(s) 248, 288, 346, 580, 754, 1236
Alw26I GTCTC 1 cut(s) 1203
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 975
AoxI GGCC 2 cut(s) 363, 779
ApeKI GCWGC 1 cut(s) 1037
ApoI RAATTY 2 cut(s) 352, 433
AspS9I GGNCC 3 cut(s) 363, 627, 1219
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 1 cut(s) 845
AvaI CYCGRG 1 cut(s) 975
AvaII GGWCC 2 cut(s) 627, 1219
BalI TGGCCA 1 cut(s) 781
BbsI GAAGAC 2 cut(s) 411, 881
BbvI GCAGC 1 cut(s) 1024
BccI CCATC 3 cut(s) 656, 929, 1058
BceAI ACGGC 1 cut(s) 809
BciT130I CCWGG 1 cut(s) 962
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 1 cut(s) 1203
BfaI CTAG 2 cut(s) 203, 1041
BfmI CTRYAG 1 cut(s) 1241
BfuAI ACCTGC 1 cut(s) 1099
BisI GCNGC 3 cut(s) 721, 1038, 1139
BlsI GCNGC 3 cut(s) 722, 1039, 1140
Bme1390I CCNGG 2 cut(s) 625, 962
Bme18I GGWCC 2 cut(s) 627, 1219
BmeT110I CYCGRG 1 cut(s) 975
BmgT120I GGNCC 3 cut(s) 363, 627, 1219
BmiI GGNNCC 1 cut(s) 1220
BmrFI CCNGG 2 cut(s) 625, 962
BmsI GCATC 3 cut(s) 713, 835, 937
BpiI GAAGAC 2 cut(s) 411, 881
Bpu10I CCTNAGC 1 cut(s) 885
BpuMI CCSGG 1 cut(s) 625
BsaAI YACGTR 1 cut(s) 1263
BsaJI CCNNGG 2 cut(s) 155, 776
BsaWI WCCGGW 2 cut(s) 953, 1169
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 981, 982
Bse1I ACTGG 4 cut(s) 321, 537, 782, 1007
Bse3DI GCAATG 2 cut(s) 848, 1001
BseBI CCWGG 1 cut(s) 962
BseDI CCNNGG 2 cut(s) 155, 776
BseGI GGATG 5 cut(s) 13, 43, 228, 704, 928
BseLI CCNNNNNNNGG 3 cut(s) 239, 981, 982
BseMI GCAATG 2 cut(s) 848, 1001
BseMII CTCAG 3 cut(s) 338, 432, 533
BseNI ACTGG 4 cut(s) 321, 537, 782, 1007
BseXI GCAGC 1 cut(s) 1024
BsgI GTGCAG 1 cut(s) 102
BshFI GGCC 2 cut(s) 365, 781
BsiHKCI CYCGRG 1 cut(s) 975
BsiSI CCGG 3 cut(s) 625, 954, 1170
BsiWI CGTACG 1 cut(s) 1263
BslFI GGGAC 3 cut(s) 29, 998, 1232
BslI CCNNNNNNNGG 3 cut(s) 239, 981, 982
BsmAI GTCTC 1 cut(s) 1203
BsmFI GGGAC 3 cut(s) 29, 998, 1232
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 2 cut(s) 365, 781
BsoBI CYCGRG 1 cut(s) 975
Bsp143I GATC 2 cut(s) 808, 835
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 3 cut(s) 720, 1094, 1138
BspANI GGCC 2 cut(s) 365, 781
BspCNI CTCAG 3 cut(s) 339, 433, 532
BspLI GGNNCC 1 cut(s) 1220
BspMI ACCTGC 1 cut(s) 1099
BsrDI GCAATG 2 cut(s) 848, 1001
BsrI ACTGG 4 cut(s) 321, 537, 782, 1007
BssECI CCNNGG 2 cut(s) 155, 776
BssMI GATC 2 cut(s) 808, 835
BssT1I CCWWGG 2 cut(s) 155, 776
Bst2UI CCWGG 1 cut(s) 962
Bst4CI ACNGT 2 cut(s) 210, 1245
BstBAI YACGTR 1 cut(s) 1263
BstC8I GCNNGC 2 cut(s) 1234, 1238
BstDEI CTNAG 4 cut(s) 347, 441, 519, 885
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 5 cut(s) 13, 43, 228, 704, 928
BstKTI GATC 2 cut(s) 811, 838
BstMAI GTCTC 1 cut(s) 1203
BstMBI GATC 2 cut(s) 808, 835
BstMWI GCNNNNNNNGC 1 cut(s) 1091
BstNI CCWGG 1 cut(s) 962
BstNSI RCATGY 4 cut(s) 22, 91, 467, 488
BstSCI CCNGG 2 cut(s) 623, 960
BstSFI CTRYAG 1 cut(s) 1241
BstSNI TACGTA 1 cut(s) 1263
BstV1I GCAGC 1 cut(s) 1024
BstV2I GAAGAC 2 cut(s) 411, 881
BsuRI GGCC 2 cut(s) 365, 781
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 5 cut(s) 13, 43, 228, 704, 928
BtsIMutI CAGTG 2 cut(s) 530, 789
BveI ACCTGC 1 cut(s) 1099
Cac8I GCNNGC 2 cut(s) 1234, 1238
CaiI CAGNNNCTG 1 cut(s) 1013
Cfr13I GGNCC 3 cut(s) 363, 627, 1219
CseI GACGC 1 cut(s) 906
Csp6I GTAC 3 cut(s) 211, 621, 1264
CviQI GTAC 3 cut(s) 211, 621, 1264
DdeI CTNAG 4 cut(s) 347, 441, 519, 885
DpnI GATC 2 cut(s) 810, 837
DpnII GATC 2 cut(s) 808, 835
EaeI YGGCCR 1 cut(s) 779
Eco105I TACGTA 1 cut(s) 1263
Eco130I CCWWGG 2 cut(s) 155, 776
Eco32I GATATC 2 cut(s) 1167, 1271
Eco47I GGWCC 2 cut(s) 627, 1219
Eco57I CTGAAG 1 cut(s) 1040
Eco88I CYCGRG 1 cut(s) 975
EcoRII CCWGG 1 cut(s) 960
EcoRV GATATC 2 cut(s) 1167, 1271
EcoT14I CCWWGG 2 cut(s) 155, 776
EcoT22I ATGCAT 3 cut(s) 414, 696, 850
ErhI CCWWGG 2 cut(s) 155, 776
FalI AAGNNNNNCTT 4 cut(s) 564, 596, 1013, 1045
FaqI GGGAC 3 cut(s) 29, 998, 1232
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 3 cut(s) 721, 1038, 1139
FokI GGATG 5 cut(s) 20, 50, 215, 691, 915
Fsp4HI GCNGC 3 cut(s) 721, 1038, 1139
FspBI CTAG 2 cut(s) 203, 1041
GluI GCNGC 3 cut(s) 721, 1038, 1139
HaeIII GGCC 2 cut(s) 365, 781
HapII CCGG 3 cut(s) 625, 954, 1170
HgaI GACGC 1 cut(s) 906
HinfI GANTC 9 cut(s) 517, 760, 787, 860, 978, 1183, 1196, 1212, 1277
HpaII CCGG 3 cut(s) 625, 954, 1170
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 2 cut(s) 253, 385
Hpy188I TCNGA 6 cut(s) 25, 106, 442, 522, 1020, 1063
Hpy188III TCNNGA 7 cut(s) 74, 596, 902, 932, 975, 1187, 1274
Hpy8I GTNNAC 2 cut(s) 253, 385
HpyAV CCTTC 2 cut(s) 5, 344
HpyCH4III ACNGT 2 cut(s) 210, 1245
HpyCH4IV ACGT 2 cut(s) 110, 1262
HpyF10VI GCNNNNNNNGC 1 cut(s) 1091
HpyF3I CTNAG 4 cut(s) 347, 441, 519, 885
HpySE526I ACGT 2 cut(s) 110, 1262
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 2 cut(s) 808, 835
Lsp1109I GCAGC 1 cut(s) 1024
LweI GCATC 3 cut(s) 713, 835, 937
MaeI CTAG 2 cut(s) 203, 1041
MaeII ACGT 2 cut(s) 110, 1262
MalI GATC 2 cut(s) 810, 837
MboI GATC 2 cut(s) 808, 835
MboII GAAGA 5 cut(s) 59, 292, 411, 886, 1033
MlsI TGGCCA 1 cut(s) 781
MluCI AATT 6 cut(s) 217, 352, 433, 451, 547, 1047
MluNI TGGCCA 1 cut(s) 781
MlyI GAGTC 4 cut(s) 511, 987, 1192, 1205
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 7 cut(s) 28, 37, 192, 398, 436, 496, 1248
Mox20I TGGCCA 1 cut(s) 781
Mph1103I ATGCAT 3 cut(s) 414, 696, 850
MscI TGGCCA 1 cut(s) 781
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 2 cut(s) 843, 933
Msp20I TGGCCA 1 cut(s) 781
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 3 cut(s) 625, 954, 1170
MspR9I CCNGG 2 cut(s) 625, 962
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 1 cut(s) 962
MwoI GCNNNNNNNGC 1 cut(s) 1091
NciI CCSGG 1 cut(s) 625
NcoI CCATGG 1 cut(s) 155
NdeII GATC 2 cut(s) 808, 835
NlaIV GGNNCC 1 cut(s) 1220
NsiI ATGCAT 3 cut(s) 414, 696, 850
NspI RCATGY 4 cut(s) 22, 91, 467, 488
PciI ACATGT 2 cut(s) 18, 463
PctI GAATGC 1 cut(s) 696
PfeI GAWTC 5 cut(s) 760, 787, 860, 1212, 1277
Pfl23II CGTACG 1 cut(s) 1263
PkrI GCNGC 3 cut(s) 722, 1039, 1140
PleI GAGTC 4 cut(s) 511, 986, 1191, 1204
PpsI GAGTC 4 cut(s) 511, 986, 1191, 1204
Ppu21I YACGTR 1 cut(s) 1263
PscI ACATGT 2 cut(s) 18, 463
Psp6I CCWGG 1 cut(s) 960
PspGI CCWGG 1 cut(s) 960
PspLI CGTACG 1 cut(s) 1263
PspN4I GGNNCC 1 cut(s) 1220
PspPI GGNCC 3 cut(s) 363, 627, 1219
PstNI CAGNNNCTG 1 cut(s) 1013
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 3 cut(s) 212, 622, 1265
RsaNI GTAC 3 cut(s) 211, 621, 1264
RseI CAYNNNNRTG 2 cut(s) 843, 933
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 721, 1038, 1139
Sau3AI GATC 2 cut(s) 808, 835
Sau96I GGNCC 3 cut(s) 363, 627, 1219
SchI GAGTC 4 cut(s) 511, 987, 1192, 1205
ScrFI CCNGG 2 cut(s) 625, 962
SfaNI GCATC 3 cut(s) 713, 835, 937
SfcI CTRYAG 1 cut(s) 1241
SinI GGWCC 2 cut(s) 627, 1219
SmiMI CAYNNNNRTG 2 cut(s) 843, 933
SnaBI TACGTA 1 cut(s) 1263
Sse9I AATT 6 cut(s) 217, 352, 433, 451, 547, 1047
SsiI CCGC 3 cut(s) 720, 1094, 1138
SspMI CTAG 2 cut(s) 203, 1041
StyD4I CCNGG 2 cut(s) 623, 960
StyI CCWWGG 2 cut(s) 155, 776
TaaI ACNGT 2 cut(s) 210, 1245
TaiI ACGT 2 cut(s) 113, 1265
TaqII GACCGA 1 cut(s) 1236
TasI AATT 6 cut(s) 217, 352, 433, 451, 547, 1047
TatI WGTACW 1 cut(s) 210
TauI GCSGC 2 cut(s) 723, 1141
TfiI GAWTC 5 cut(s) 760, 787, 860, 1212, 1277
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 537, 789
TseI GCWGC 1 cut(s) 1037
TspDTI ATGAA 6 cut(s) 24, 93, 326, 688, 1047, 1063
TspGWI ACGGA 1 cut(s) 1281
TspRI CASTG 2 cut(s) 537, 789
VpaK11BI GGWCC 2 cut(s) 627, 1219
XapI RAATTY 2 cut(s) 352, 433
XceI RCATGY 4 cut(s) 22, 91, 467, 488
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 2 cut(s) 203, 1041
Zsp2I ATGCAT 3 cut(s) 414, 696, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.