Rw0G014370

WD repeat-containing protein C2A9.03-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00646
OG0003717
Physical Location & Seq
Reverse (-)
13370 .. 14986
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014370.1

Sequence Viewer

Length: 600 bp
ATGCATTTCATGGCATCCAATAATGATTGTGGTATAAGAGAATATGACATGGAGAGATTTCAGCTTCTGAATCACTTTCGATTCCCCTGGCCAGTGAATCATACATCAATAAGCCCAGATCGTAGGGTTGTTGCCGTAGTTGGAGATCACGTGGATGGATTGCTTATGGATTCTCAGAATGGAAAGACTATTGCTAAGGTTGTTGGTCATCTAGATTACTCCTTTGCTTCTGCATGGCATCCTGATGGCCGCATATTTGCCACCGGAAATCAGGACAAGACTTCCCGAGTTTGGGATATAAGAAATCTTTCATCACCCATTGCAGTTCTGAAGAATAACTTGGGTGCTACTCGGTCTATTCGGTTTTCATCAGATGGTCAGTTTATGATGGTTGCAGAACCTGCAGATTTTGTGCATATATATAGTACAAGGGCAGATTACAAAAAACGGCAAGAGATTGATTTCTTTGGAGAGATTTCGGGGGTATCAGCAAGTCCAGATGACGAGTCCTTGTACATAGGAATCTGGGACCGAACATACGCAAGCTTGTTGCAATATAACAGAAGGCATACATATGGATATCTTGATTCCTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

199

Amino Acids

22.83

Weight (kDa)

6.22

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 4 - 170 5e-17 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 4 - 147 2.4e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 7 - 145 6.2e-12 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 24 - 144 2.4e-06 WDHD1 first WD40 domain
WD40_Gbeta PF25391 29 - 137 2.8e-06 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 31 - 156 1.6e-14 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 32 - 178 2.3e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 35 - 145 3.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 76 - 153 1.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
AciI CCGC 1 cut(s) 250
AcoI YGGCCR 2 cut(s) 89, 247
AcuI CTGAAG 1 cut(s) 350
AcvI CACGTG 1 cut(s) 151
AfaI GTAC 2 cut(s) 427, 515
AfiI CCNNNNNNNGG 2 cut(s) 291, 292
AjnI CCWGG 1 cut(s) 86
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 2 cut(s) 64, 546
AluI AGCT 2 cut(s) 64, 546
AlwNI CAGNNNCTG 2 cut(s) 67, 401
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 2 cut(s) 89, 247
Asp700I GAANNNNTTC 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 529
AsuHPI GGTGA 1 cut(s) 306
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 1 cut(s) 529
BalI TGGCCA 1 cut(s) 91
BbrPI CACGTG 1 cut(s) 151
BccI CCATC 4 cut(s) 149, 239, 368, 382
BceAI ACGGC 2 cut(s) 119, 464
BciT130I CCWGG 1 cut(s) 88
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 409
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 88
Bme18I GGWCC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 530
BmrFI CCNGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 23, 247
Bpu10I CCTNAGC 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 86
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 292
Bse1I ACTGG 1 cut(s) 92
Bse3DI GCAATG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 1 cut(s) 86
BseGI GGATG 3 cut(s) 14, 160, 238
BseLI CCNNNNNNNGG 2 cut(s) 291, 292
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 92
BshFI GGCC 2 cut(s) 91, 249
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 2 cut(s) 291, 292
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 2 cut(s) 91, 249
BsoBI CYCGRG 1 cut(s) 285
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 2 cut(s) 118, 145
BspACI CCGC 1 cut(s) 250
BspANI GGCC 2 cut(s) 91, 249
BspCNI CTCAG 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 530
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 318
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 2 cut(s) 118, 145
Bst2UI CCWGG 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 401
BstAUI TGTACA 1 cut(s) 513
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 544
BstDEI CTNAG 2 cut(s) 174, 195
BstF5I GGATG 3 cut(s) 14, 160, 238
BstKTI GATC 2 cut(s) 121, 148
BstMBI GATC 2 cut(s) 118, 145
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 402
BsuRI GGCC 2 cut(s) 91, 249
BtsCI GGATG 3 cut(s) 14, 160, 238
BtsIMutI CAGTG 1 cut(s) 99
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 544
CaiI CAGNNNCTG 2 cut(s) 67, 401
Cfr13I GGNCC 1 cut(s) 529
Csp6I GTAC 2 cut(s) 426, 514
CviAII CATG 3 cut(s) 10, 49, 234
CviJI RGCY 5 cut(s) 64, 91, 114, 249, 546
CviKI_1 RGCY 5 cut(s) 64, 91, 114, 249, 546
CviQI GTAC 2 cut(s) 426, 514
DdeI CTNAG 2 cut(s) 174, 195
DpnI GATC 2 cut(s) 120, 147
DpnII GATC 2 cut(s) 118, 145
EaeI YGGCCR 2 cut(s) 89, 247
Eco32I GATATC 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 350
Eco72I CACGTG 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 86
EcoRV GATATC 1 cut(s) 581
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 13, 52, 237
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 1 cut(s) 542
FatI CATG 3 cut(s) 9, 48, 233
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 2 cut(s) 167, 225
Fsp4HI GCNGC 1 cut(s) 250
FspBI CTAG 1 cut(s) 212
GluI GCNGC 1 cut(s) 250
HaeIII GGCC 2 cut(s) 91, 249
HapII CCGG 1 cut(s) 264
Hin1II CATG 3 cut(s) 13, 52, 237
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 7 cut(s) 70, 81, 97, 170, 506, 522, 587
HpaII CCGG 1 cut(s) 264
HphI GGTGA 1 cut(s) 306
Hpy188I TCNGA 4 cut(s) 69, 177, 330, 373
Hpy188III TCNNGA 6 cut(s) 212, 242, 272, 285, 497, 584
HpyAV CCTTC 1 cut(s) 558
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 7 cut(s) 4, 233, 323, 395, 404, 415, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 2 cut(s) 174, 195
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 13, 52, 237
Kzo9I GATC 2 cut(s) 118, 145
LweI GCATC 2 cut(s) 23, 247
MaeI CTAG 1 cut(s) 212
MaeII ACGT 1 cut(s) 150
MalI GATC 2 cut(s) 120, 147
MboI GATC 2 cut(s) 118, 145
MboII GAAGA 1 cut(s) 343
MlsI TGGCCA 1 cut(s) 91
MluNI TGGCCA 1 cut(s) 91
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 121
Mox20I TGGCCA 1 cut(s) 91
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 307
MscI TGGCCA 1 cut(s) 91
MslI CAYNNNNRTG 3 cut(s) 153, 243, 573
Msp20I TGGCCA 1 cut(s) 91
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 88
MvaI CCWGG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 401
NdeI CATATG 1 cut(s) 574
NdeII GATC 2 cut(s) 118, 145
NlaIII CATG 3 cut(s) 13, 52, 237
NlaIV GGNNCC 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 307
PfeI GAWTC 6 cut(s) 70, 81, 97, 170, 522, 587
PkrI GCNGC 1 cut(s) 251
PleI GAGTC 1 cut(s) 514
PmaCI CACGTG 1 cut(s) 151
PmlI CACGTG 1 cut(s) 151
PpsI GAGTC 1 cut(s) 514
Ppu21I YACGTR 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 86
PspCI CACGTG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 530
PspPI GGNCC 1 cut(s) 529
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 2 cut(s) 67, 401
RsaI GTAC 2 cut(s) 427, 515
RsaNI GTAC 2 cut(s) 426, 514
RseI CAYNNNNRTG 3 cut(s) 153, 243, 573
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 2 cut(s) 118, 145
Sau96I GGNCC 1 cut(s) 529
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 1 cut(s) 88
SetI ASST 5 cut(s) 66, 153, 201, 403, 548
SfaNI GCATC 2 cut(s) 23, 247
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 1 cut(s) 529
SmiMI CAYNNNNRTG 3 cut(s) 153, 243, 573
SsiI CCGC 1 cut(s) 250
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 86
TaiI ACGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 79
TaqII GACCGA 2 cut(s) 342, 546
TatI WGTACW 2 cut(s) 425, 513
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 6 cut(s) 70, 81, 97, 170, 522, 587
TscAI CASTG 1 cut(s) 99
TspDTI ATGAA 2 cut(s) 300, 357
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 529
XbaI TCTAGA 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 307
XspI CTAG 1 cut(s) 212
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.