Rh4AG160800

WD repeat-containing protein C2A9.03-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003717
Physical Location & Seq
Reverse (-)
40921877 .. 40927927
6051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG160800.1

Sequence Viewer

Length: 1335 bp
ATGGCTTTTGAGCAGCCTCATCAGTTTGAATATATGGCAGATGAGGGTGACATGTCTGATTTCGTTGAGGACGCAGATGTGGATGCCGATGAAGAAAATCACGGTAGAGATATGGTTCTTGACGAATACGACATGCTTAACCAGGTGTCGGATACGTCTTCTGCCCAAGCAAGAAAAGGAAAGGATATTCAGGGTATACCGTGGGATAGGTTAAACATAACGAGGGAGAAGTACAGATTGACGAGGCTTGAACAGTACAAGAATTATGAGAATATCCCTTCATCTGGGGAAGCTGTGGACAAGGAATGTCAACTAATGCAGAAGGGTGGCAACTACTATGAATTCTTCCACAACACAAGACTGGTTAAACCTACAATCCTTCATTTTCAGCTCAGAAATCTGGTTTGGGCTACTTCGAAACATGACGTCTATCTCATGTCCAATTATTCAGTTATGCATTGGTCTTCTCTATCTGGCAATTTGTCTGAGGTCCTCAATTTTTCAGGACATGTTGCACCAACTGAGAAACATGCAGGAAGTTTGTTGGAGGGTTTTATGCAGACTCAGATAAGCACCCTAGCAGTCAAGGACAATTTTCTTGTTGCTGGAGGTTTCCAAGGAGAGCTTACTTGCAAGCGTCTTGATAAAGAAGGAGTGAGTTTTTGTACGCGAACCACATACGATGACAATGCCATCACAAATGCTGTTGAAATATATGATAGCCTGGGGGGTGGAATGCATTTCATGGCATCCAATAATGATTGTGGTATAAGAGAATATGACATGGAGAGATTTCAGCTTCTGAATCACTTTCGATTCCCCTGGCCAGTGAATCATACATCAATAAGCCCAGATCGTAGGGTTGTTGCTGTAGTTGGAGATCACGTGGATGGATTGCTTATGGATTCTCAGAATGGAAAGACTATTGCTAAGGTTGTTGGTCATCTAGATTACTCCTTTGCTTCTGCATGGCATCCTGATGGCCGCATATTTGCCACCGGAAATCAGGACAAGACTTCCCGAGTTTGGGATATAAGAAATCTTTCATCACCCATTGCAGTTCTGAAGAATAACTTGGGTGCTACTCGGTCTATTCGGTTTTCATCAGATGGTCAGTTTATGATGGTTGCAGAACCTGCAGATTTTGTGCATATATATAGTACAAGGGCAGATTACAAAAAACGGCAAGAGATTGATTTCTTTGGAGAGATTTCGGGGGTATCAGCAAGTCCAGATGACGAGTCCTTGTACATAGGAATCTGGGACCGAACATACGCAAGCTTGTTGCAATATAACAGAAGGCATACATATGGATATCTTGATTCCTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

444

Amino Acids

50.64

Weight (kDa)

5.2

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 245 - 415 1.9e-16 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 246 - 392 7.3e-10 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 248 - 388 7.8e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 249 - 390 3.1e-11 WDR5 beta-propeller domain
WD40_Prp19 PF24814 276 - 400 6.4e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 321 - 398 4.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
Acc36I ACCTGC 1 cut(s) 1144
AccI GTMKAC 1 cut(s) 196
AccII CGCG 1 cut(s) 670
AciI CCGC 1 cut(s) 985
AcoI YGGCCR 2 cut(s) 824, 982
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 1085
AcvI CACGTG 1 cut(s) 886
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 5 cut(s) 233, 257, 667, 1162, 1250
AfiI CCNNNNNNNGG 4 cut(s) 148, 284, 1026, 1027
AflIII ACRYGT 2 cut(s) 51, 508
AgsI TTSAA 3 cut(s) 29, 251, 710
AjnI CCWGG 3 cut(s) 141, 723, 821
AjuI GAANNNNNNNTTGG 4 cut(s) 388, 420, 1058, 1090
AluBI AGCT 5 cut(s) 293, 391, 625, 799, 1281
AluI AGCT 5 cut(s) 293, 391, 625, 799, 1281
AlwNI CAGNNNCTG 2 cut(s) 802, 1136
Ama87I CYCGRG 1 cut(s) 1020
AoxI GGCC 2 cut(s) 824, 982
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 1042
AspS9I GGNCC 2 cut(s) 490, 1264
AsuHPI GGTGA 2 cut(s) 59, 1041
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 1020
AvaII GGWCC 2 cut(s) 490, 1264
BalI TGGCCA 1 cut(s) 826
BbrPI CACGTG 1 cut(s) 886
BbsI GAAGAC 2 cut(s) 150, 456
BbvI GCAGC 1 cut(s) 25
BccI CCATC 5 cut(s) 701, 884, 974, 1103, 1117
BceAI ACGGC 1 cut(s) 1199
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 3 cut(s) 143, 725, 823
BciVI GTATCC 1 cut(s) 145
BfaI CTAG 2 cut(s) 578, 947
BfmI CTRYAG 2 cut(s) 870, 1137
BfuAI ACCTGC 1 cut(s) 1144
BfuI GTATCC 1 cut(s) 145
BisI GCNGC 2 cut(s) 14, 985
BlsI GCNGC 2 cut(s) 15, 986
Bme1390I CCNGG 3 cut(s) 143, 725, 823
Bme18I GGWCC 2 cut(s) 490, 1264
BmeT110I CYCGRG 1 cut(s) 1020
BmgT120I GGNCC 2 cut(s) 490, 1264
BmiI GGNNCC 1 cut(s) 1265
BmrFI CCNGG 3 cut(s) 143, 725, 823
BmsI GCATC 3 cut(s) 73, 758, 982
BpiI GAAGAC 2 cut(s) 150, 456
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 930
Bpu14I TTCGAA 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 886
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 200, 616, 724, 821
BsaWI WCCGGW 1 cut(s) 998
BsaXI ACNNNNNCTCC 2 cut(s) 870, 900
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 284, 1026, 1027
Bse1I ACTGG 2 cut(s) 366, 827
Bse3DI GCAATG 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 143, 725, 823
BseDI CCNNGG 4 cut(s) 200, 616, 724, 821
BseGI GGATG 4 cut(s) 88, 749, 895, 973
BseLI CCNNNNNNNGG 4 cut(s) 148, 284, 1026, 1027
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 5 cut(s) 406, 477, 513, 578, 923
BseNI ACTGG 2 cut(s) 366, 827
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 670
BshFI GGCC 2 cut(s) 826, 984
BsiHKCI CYCGRG 1 cut(s) 1020
BsiSI CCGG 1 cut(s) 999
BslFI GGGAC 1 cut(s) 1277
BslI CCNNNNNNNGG 4 cut(s) 148, 284, 1026, 1027
BsmFI GGGAC 1 cut(s) 1277
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 2 cut(s) 826, 984
BsoBI CYCGRG 1 cut(s) 1020
Bsp119I TTCGAA 1 cut(s) 416
Bsp1407I TGTACA 1 cut(s) 1248
Bsp143I GATC 2 cut(s) 853, 880
BspACI CCGC 1 cut(s) 985
BspANI GGCC 2 cut(s) 826, 984
BspCNI CTCAG 5 cut(s) 405, 478, 514, 577, 922
BspFNI CGCG 1 cut(s) 670
BspLI GGNNCC 1 cut(s) 1265
BspMAI CTGCAG 1 cut(s) 1141
BspMI ACCTGC 1 cut(s) 1144
BspT104I TTCGAA 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 1053
BsrGI TGTACA 1 cut(s) 1248
BsrI ACTGG 2 cut(s) 366, 827
BssECI CCNNGG 4 cut(s) 200, 616, 724, 821
BssMI GATC 2 cut(s) 853, 880
BssNAI GTATAC 1 cut(s) 197
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 616
Bst1107I GTATAC 1 cut(s) 197
Bst2UI CCWGG 3 cut(s) 143, 725, 823
Bst4CI ACNGT 3 cut(s) 104, 201, 255
BstACI GRCGYC 1 cut(s) 426
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstAUI TGTACA 1 cut(s) 1248
BstBAI YACGTR 1 cut(s) 886
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 635, 1279
BstDEI CTNAG 6 cut(s) 392, 486, 522, 564, 909, 930
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 4 cut(s) 88, 749, 895, 973
BstFNI CGCG 1 cut(s) 670
BstKTI GATC 2 cut(s) 856, 883
BstMBI GATC 2 cut(s) 853, 880
BstMWI GCNNNNNNNGC 1 cut(s) 1136
BstNI CCWGG 3 cut(s) 143, 725, 823
BstNSI RCATGY 4 cut(s) 55, 136, 512, 533
BstSCI CCNGG 3 cut(s) 141, 723, 821
BstSFI CTRYAG 2 cut(s) 870, 1137
BstUI CGCG 1 cut(s) 670
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 150, 456
BstZ17I GTATAC 1 cut(s) 197
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 2 cut(s) 826, 984
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 4 cut(s) 88, 749, 895, 973
BtsIMutI CAGTG 1 cut(s) 834
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 2 cut(s) 635, 1279
CaiI CAGNNNCTG 2 cut(s) 802, 1136
Cfr13I GGNCC 2 cut(s) 490, 1264
CseI GACGC 2 cut(s) 80, 626
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 5 cut(s) 232, 256, 666, 1161, 1249
CviAII CATG 9 cut(s) 52, 133, 422, 436, 509, 530, 745, 784, 969
CviQI GTAC 5 cut(s) 232, 256, 666, 1161, 1249
DdeI CTNAG 6 cut(s) 392, 486, 522, 564, 909, 930
DpnI GATC 2 cut(s) 855, 882
DpnII GATC 2 cut(s) 853, 880
EaeI YGGCCR 2 cut(s) 824, 982
Eco130I CCWWGG 1 cut(s) 616
Eco32I GATATC 1 cut(s) 1316
Eco47I GGWCC 2 cut(s) 490, 1264
Eco57I CTGAAG 1 cut(s) 1085
Eco72I CACGTG 1 cut(s) 886
Eco88I CYCGRG 1 cut(s) 1020
EcoO109I RGGNCCY 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 341
EcoRII CCWGG 3 cut(s) 141, 723, 821
EcoRV GATATC 1 cut(s) 1316
EcoT14I CCWWGG 1 cut(s) 616
EcoT22I ATGCAT 2 cut(s) 459, 741
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 9 cut(s) 55, 136, 425, 439, 512, 533, 748, 787, 972
FalI AAGNNNNNCTT 4 cut(s) 609, 641, 1058, 1090
FaqI GGGAC 1 cut(s) 1277
FatI CATG 9 cut(s) 51, 132, 421, 435, 508, 529, 744, 783, 968
FauNDI CATATG 1 cut(s) 1309
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 14, 985
FokI GGATG 4 cut(s) 95, 736, 902, 960
Fsp4HI GCNGC 2 cut(s) 14, 985
FspBI CTAG 2 cut(s) 578, 947
GluI GCNGC 2 cut(s) 14, 985
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 2 cut(s) 826, 984
HapII CCGG 1 cut(s) 999
HgaI GACGC 2 cut(s) 80, 626
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 9 cut(s) 55, 136, 425, 439, 512, 533, 748, 787, 972
HincII GTYRAC 1 cut(s) 311
HindII GTYRAC 1 cut(s) 311
HindIII AAGCTT 1 cut(s) 1279
HinfI GANTC 8 cut(s) 562, 805, 816, 832, 905, 1241, 1257, 1322
HpaII CCGG 1 cut(s) 999
HphI GGTGA 2 cut(s) 59, 1041
Hpy166II GTNNAC 3 cut(s) 197, 298, 311
Hpy188I TCNGA 9 cut(s) 58, 151, 395, 487, 567, 804, 912, 1065, 1108
Hpy188III TCNNGA 9 cut(s) 119, 504, 641, 947, 977, 1007, 1020, 1232, 1319
Hpy8I GTNNAC 3 cut(s) 197, 298, 311
HpyAV CCTTC 5 cut(s) 288, 316, 389, 644, 1293
HpyCH4III ACNGT 3 cut(s) 104, 201, 255
HpyCH4IV ACGT 3 cut(s) 155, 426, 885
HpyF10VI GCNNNNNNNGC 1 cut(s) 1136
HpyF3I CTNAG 6 cut(s) 392, 486, 522, 564, 909, 930
HpySE526I ACGT 3 cut(s) 155, 426, 885
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 9 cut(s) 55, 136, 425, 439, 512, 533, 748, 787, 972
Kzo9I GATC 2 cut(s) 853, 880
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 3 cut(s) 73, 758, 982
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 2 cut(s) 578, 947
MaeII ACGT 3 cut(s) 155, 426, 885
MaeIII GTNAC 1 cut(s) 47
MalI GATC 2 cut(s) 855, 882
MboI GATC 2 cut(s) 853, 880
MboII GAAGA 5 cut(s) 104, 150, 337, 456, 1078
MlsI TGGCCA 1 cut(s) 826
MluCI AATT 6 cut(s) 262, 341, 442, 478, 496, 592
MluNI TGGCCA 1 cut(s) 826
MlyI GAGTC 2 cut(s) 556, 1250
MmeI TCCRAC 3 cut(s) 129, 525, 856
MnlI CCTC 9 cut(s) 27, 37, 61, 216, 237, 481, 503, 541, 602
Mox20I TGGCCA 1 cut(s) 826
Mph1103I ATGCAT 2 cut(s) 459, 741
MroXI GAANNNNTTC 1 cut(s) 1042
MscI TGGCCA 1 cut(s) 826
MseI TTAA 3 cut(s) 138, 212, 366
MslI CAYNNNNRTG 3 cut(s) 888, 978, 1308
Msp20I TGGCCA 1 cut(s) 826
MspI CCGG 1 cut(s) 999
MspR9I CCNGG 3 cut(s) 143, 725, 823
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 3 cut(s) 143, 725, 823
MvnI CGCG 1 cut(s) 670
MwoI GCNNNNNNNGC 1 cut(s) 1136
NdeI CATATG 1 cut(s) 1309
NdeII GATC 2 cut(s) 853, 880
NlaIII CATG 9 cut(s) 55, 136, 425, 439, 512, 533, 748, 787, 972
NlaIV GGNNCC 1 cut(s) 1265
NmuCI GTSAC 1 cut(s) 47
NsiI ATGCAT 2 cut(s) 459, 741
NspI RCATGY 4 cut(s) 55, 136, 512, 533
NspV TTCGAA 1 cut(s) 416
PciI ACATGT 2 cut(s) 51, 508
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 1042
PfeI GAWTC 6 cut(s) 805, 816, 832, 905, 1257, 1322
PkrI GCNGC 2 cut(s) 15, 986
PleI GAGTC 2 cut(s) 556, 1249
PmaCI CACGTG 1 cut(s) 886
PmlI CACGTG 1 cut(s) 886
PpsI GAGTC 2 cut(s) 556, 1249
Ppu21I YACGTR 1 cut(s) 886
PpuMI RGGWCCY 1 cut(s) 490
PscI ACATGT 2 cut(s) 51, 508
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 3 cut(s) 141, 723, 821
PspCI CACGTG 1 cut(s) 886
PspGI CCWGG 3 cut(s) 141, 723, 821
PspN4I GGNNCC 1 cut(s) 1265
PspPI GGNCC 2 cut(s) 490, 1264
PspPPI RGGWCCY 1 cut(s) 490
PstI CTGCAG 1 cut(s) 1141
PstNI CAGNNNCTG 2 cut(s) 802, 1136
RsaI GTAC 5 cut(s) 233, 257, 667, 1162, 1250
RsaNI GTAC 5 cut(s) 232, 256, 666, 1161, 1249
RseI CAYNNNNRTG 3 cut(s) 888, 978, 1308
SaqAI TTAA 3 cut(s) 138, 212, 366
SatI GCNGC 2 cut(s) 14, 985
Sau3AI GATC 2 cut(s) 853, 880
Sau96I GGNCC 2 cut(s) 490, 1264
SchI GAGTC 2 cut(s) 556, 1250
ScrFI CCNGG 3 cut(s) 143, 725, 823
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 3 cut(s) 73, 758, 982
SfcI CTRYAG 2 cut(s) 870, 1137
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 2 cut(s) 490, 1264
SmiMI CAYNNNNRTG 3 cut(s) 888, 978, 1308
Sse9I AATT 6 cut(s) 262, 341, 442, 478, 496, 592
SsiI CCGC 1 cut(s) 985
SspMI CTAG 2 cut(s) 578, 947
StyD4I CCNGG 3 cut(s) 141, 723, 821
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 3 cut(s) 104, 201, 255
TaiI ACGT 3 cut(s) 158, 429, 888
TaqI TCGA 2 cut(s) 416, 814
TaqII GACCGA 2 cut(s) 1077, 1281
TasI AATT 6 cut(s) 262, 341, 442, 478, 496, 592
TatI WGTACW 4 cut(s) 231, 255, 1160, 1248
TauI GCSGC 1 cut(s) 987
TfiI GAWTC 6 cut(s) 805, 816, 832, 905, 1257, 1322
Tru1I TTAA 3 cut(s) 138, 212, 366
Tru9I TTAA 3 cut(s) 138, 212, 366
TscAI CASTG 1 cut(s) 834
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 7 cut(s) 105, 270, 354, 371, 733, 1035, 1092
TspRI CASTG 1 cut(s) 834
VpaK11BI GGWCC 2 cut(s) 490, 1264
XapI RAATTY 1 cut(s) 341
XbaI TCTAGA 1 cut(s) 946
XceI RCATGY 4 cut(s) 55, 136, 512, 533
XmiI GTMKAC 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 1042
XspI CTAG 2 cut(s) 578, 947
ZraI GACGTC 1 cut(s) 427
Zsp2I ATGCAT 2 cut(s) 459, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.