AT5G58250

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010918
Physical Location & Seq
Forward (+)
23559474 .. 23560778
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58250.1

Sequence Viewer

Length: 636 bp
ATGTGGAGCGTCACCGGAGCATTGACTGTGGCGGTGCCGCCTACCGCCGCCGCTTGCCGTACGAAACCCTTTCTCATCTCCTCTTCCTTTCCCAAGCAGACAAAGAAACTCCATCTCTCTTCGCCTTCTCTGTCTCTACCATCATCACATTTCTCCTCCTCTTTCAAAACCGCAGCTACCTCCATCGAGCAACAGTCTTCTGTTAACAAGGGAGAGAGCACCAAGTACCATTTCCTGGTAGCAAACGCTAAATTCATGCTTGACGAAGAGGAGCACTTCCAGGAGCAATTGTTTGAACGTCTCCGTTACTTCGGTGAGCGTGAATTAGTGCAAGACTTCTGGCTTGTCATTGAGCCTAAATTCCTTGACAATTTCCCCAAAATTACTCAAAGACTTCGTCGTCCTGCTGTTGCTCTTGTTTCCACCAATGGCACCTGGATCACGTTTATGAAGTTAAGATTGGATCGTGTTCTGTATGATTCCTTTGAAGCTACCTCTCTTGATGAAGCTTTGGCCTCCAATCCTACCACTCTTGAGTTCGATAAGCCTAAAAATTGGGTGGCGCCATACCCCAAGTATGAACCTGGATGGTGGGATACCTTCTTGCCTAAAGTAACCCAAGAATCTGCAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

211

Amino Acids

24.1

Weight (kDa)

7.82

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 75 - 164 2e-32 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 399
AccB1I GGYRCC 3 cut(s) 34, 431, 562
AccB7I CCANNNNNTGG 1 cut(s) 235
AciI CCGC 6 cut(s) 32, 38, 45, 48, 51, 171
AclWI GGATC 2 cut(s) 446, 471
AcsI RAATTY 2 cut(s) 251, 359
AcyI GRCGYC 1 cut(s) 563
AfaI GTAC 2 cut(s) 61, 227
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 3 cut(s) 166, 296, 488
AjnI CCWGG 4 cut(s) 234, 279, 434, 583
AjuI GAANNNNNNNTTGG 4 cut(s) 215, 247, 443, 475
AluBI AGCT 3 cut(s) 176, 491, 509
AluI AGCT 3 cut(s) 176, 491, 509
Alw21I GWGCWC 2 cut(s) 221, 276
Alw26I GTCTC 2 cut(s) 138, 305
AlwI GGATC 2 cut(s) 446, 471
AoxI GGCC 1 cut(s) 513
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 2 cut(s) 251, 359
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
AspLEI GCGC 1 cut(s) 565
AsuHPI GGTGA 2 cut(s) 4, 326
BanI GGYRCC 3 cut(s) 34, 431, 562
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 2 cut(s) 221, 276
BbvI GCAGC 1 cut(s) 185
BccI CCATC 4 cut(s) 120, 148, 191, 582
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 4 cut(s) 236, 281, 436, 585
BciVI GTATCC 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 138, 305
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 566
BfuI GTATCC 1 cut(s) 589
BisI GCNGC 4 cut(s) 38, 48, 51, 174
BlsI GCNGC 4 cut(s) 39, 49, 52, 175
Bme1390I CCNGG 4 cut(s) 236, 281, 436, 585
BmiI GGNNCC 3 cut(s) 36, 433, 564
BmrFI CCNGG 4 cut(s) 236, 281, 436, 585
BpiI GAAGAC 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 563
BsaWI WCCGGW 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 1 cut(s) 235
BseBI CCWGG 4 cut(s) 236, 281, 436, 585
BseGI GGATG 1 cut(s) 593
BseLI CCNNNNNNNGG 1 cut(s) 235
BseRI GAGGAG 4 cut(s) 70, 145, 148, 284
BseXI GCAGC 1 cut(s) 185
BshFI GGCC 1 cut(s) 515
BshNI GGYRCC 3 cut(s) 34, 431, 562
BsiHKAI GWGCWC 2 cut(s) 221, 276
BsiSI CCGG 1 cut(s) 15
BsiWI CGTACG 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 2 cut(s) 138, 305
BsmBI CGTCTC 1 cut(s) 305
BsnI GGCC 1 cut(s) 515
Bsp1286I GDGCHC 2 cut(s) 221, 276
Bsp143I GATC 2 cut(s) 438, 463
BspACI CCGC 6 cut(s) 32, 38, 45, 48, 51, 171
BspANI GGCC 1 cut(s) 515
BspLI GGNNCC 3 cut(s) 36, 433, 564
BspMAI CTGCAG 1 cut(s) 631
BspPI GGATC 2 cut(s) 446, 471
BspT107I GGYRCC 3 cut(s) 34, 431, 562
BssMI GATC 2 cut(s) 438, 463
BssNI GRCGYC 1 cut(s) 563
Bst2UI CCWGG 4 cut(s) 236, 281, 436, 585
Bst4CI ACNGT 2 cut(s) 28, 195
Bst6I CTCTTC 3 cut(s) 88, 124, 261
BstACI GRCGYC 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 55
BstF5I GGATG 1 cut(s) 593
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 1 cut(s) 565
BstKTI GATC 2 cut(s) 441, 466
BstMAI GTCTC 2 cut(s) 138, 305
BstMBI GATC 2 cut(s) 438, 463
BstNI CCWGG 4 cut(s) 236, 281, 436, 585
BstSCI CCNGG 4 cut(s) 234, 279, 434, 583
BstSFI CTRYAG 1 cut(s) 627
BstV1I GCAGC 1 cut(s) 185
BstV2I GAAGAC 1 cut(s) 189
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 1 cut(s) 515
BtsCI GGATG 1 cut(s) 593
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 1 cut(s) 565
Csp6I GTAC 2 cut(s) 60, 226
CviAII CATG 1 cut(s) 256
CviJI RGCY 7 cut(s) 176, 343, 355, 491, 509, 515, 547
CviKI_1 RGCY 7 cut(s) 176, 343, 355, 491, 509, 515, 547
CviQI GTAC 2 cut(s) 60, 226
DinI GGCGCC 1 cut(s) 564
DpnI GATC 2 cut(s) 440, 465
DpnII GATC 2 cut(s) 438, 463
DrdI GACNNNNNNGTC 1 cut(s) 399
DseDI GACNNNNNNGTC 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 88, 124, 261
EarI CTCTTC 3 cut(s) 88, 124, 261
EcoRII CCWGG 4 cut(s) 234, 279, 434, 583
EgeI GGCGCC 1 cut(s) 564
EheI GGCGCC 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 1 cut(s) 259
FaiI YATR 6 cut(s) 257, 449, 477, 568, 579, 634
FatI CATG 1 cut(s) 255
Fnu4HI GCNGC 4 cut(s) 38, 48, 51, 174
FokI GGATG 1 cut(s) 600
Fsp4HI GCNGC 4 cut(s) 38, 48, 51, 174
GlaI GCGC 1 cut(s) 564
GluI GCNGC 4 cut(s) 38, 48, 51, 174
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 1 cut(s) 515
HapII CCGG 1 cut(s) 15
HhaI GCGC 1 cut(s) 565
Hin1I GRCGYC 1 cut(s) 563
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 1 cut(s) 563
HinP1I GCGC 1 cut(s) 563
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 507
HinfI GANTC 2 cut(s) 479, 623
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 1 cut(s) 15
HphI GGTGA 2 cut(s) 4, 326
Hpy166II GTNNAC 1 cut(s) 205
Hpy188III TCNNGA 2 cut(s) 500, 533
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 402
HpyAV CCTTC 2 cut(s) 135, 610
HpyCH4III ACNGT 2 cut(s) 28, 195
HpyCH4IV ACGT 2 cut(s) 298, 443
HpyCH4V TGCA 2 cut(s) 331, 629
HpySE526I ACGT 2 cut(s) 298, 443
Hsp92I GRCGYC 1 cut(s) 563
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 1 cut(s) 563
KasI GGCGCC 1 cut(s) 562
KspAI GTTAAC 1 cut(s) 205
Kzo9I GATC 2 cut(s) 438, 463
LmnI GCTCC 4 cut(s) 6, 17, 271, 283
Lsp1109I GCAGC 1 cut(s) 185
MaeII ACGT 2 cut(s) 298, 443
MaeIII GTNAC 3 cut(s) 10, 305, 613
MalI GATC 2 cut(s) 440, 465
MboI GATC 2 cut(s) 438, 463
MboII GAAGA 4 cut(s) 75, 111, 189, 278
MfeI CAATTG 1 cut(s) 287
MhlI GDGCHC 2 cut(s) 221, 276
MluCI AATT 7 cut(s) 251, 287, 323, 359, 370, 381, 553
Mly113I GGCGCC 1 cut(s) 563
MnlI CCTC 7 cut(s) 91, 166, 169, 190, 262, 505, 526
MseI TTAA 2 cut(s) 204, 455
MslI CAYNNNNRTG 1 cut(s) 446
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 4 cut(s) 236, 281, 436, 585
MunI CAATTG 1 cut(s) 287
MvaI CCWGG 4 cut(s) 236, 281, 436, 585
NarI GGCGCC 1 cut(s) 563
NdeII GATC 2 cut(s) 438, 463
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 3 cut(s) 36, 433, 564
NmuCI GTSAC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 479, 623
Pfl23II CGTACG 1 cut(s) 59
PflFI GACNNNGTC 1 cut(s) 396
PflMI CCANNNNNTGG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 4 cut(s) 39, 49, 52, 175
PluTI GGCGCC 1 cut(s) 566
Psp6I CCWGG 4 cut(s) 234, 279, 434, 583
PspGI CCWGG 4 cut(s) 234, 279, 434, 583
PspLI CGTACG 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 36, 433, 564
PstI CTGCAG 1 cut(s) 631
PsyI GACNNNGTC 1 cut(s) 396
RsaI GTAC 2 cut(s) 61, 227
RsaNI GTAC 2 cut(s) 60, 226
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 2 cut(s) 204, 455
SatI GCNGC 4 cut(s) 38, 48, 51, 174
Sau3AI GATC 2 cut(s) 438, 463
ScrFI CCNGG 4 cut(s) 236, 281, 436, 585
SduI GDGCHC 2 cut(s) 221, 276
SfcI CTRYAG 1 cut(s) 627
SfoI GGCGCC 1 cut(s) 564
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 7 cut(s) 251, 287, 323, 359, 370, 381, 553
SsiI CCGC 6 cut(s) 32, 38, 45, 48, 51, 171
SspDI GGCGCC 1 cut(s) 562
StyD4I CCNGG 4 cut(s) 234, 279, 434, 583
TaaI ACNGT 2 cut(s) 28, 195
TaiI ACGT 2 cut(s) 301, 446
TaqI TCGA 2 cut(s) 186, 540
TasI AATT 7 cut(s) 251, 287, 323, 359, 370, 381, 553
TauI GCSGC 3 cut(s) 40, 50, 53
TfiI GAWTC 2 cut(s) 479, 623
Tru1I TTAA 2 cut(s) 204, 455
Tru9I TTAA 2 cut(s) 204, 455
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 244, 464, 519, 594
TspGWI ACGGA 1 cut(s) 293
Tth111I GACNNNGTC 1 cut(s) 396
Van91I CCANNNNNTGG 1 cut(s) 235
XapI RAATTY 2 cut(s) 251, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.