Rh5CG529500

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010918
Physical Location & Seq
Reverse (-)
74073103 .. 74074533
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG529500.1

Sequence Viewer

Length: 666 bp
ATGGCAGCCACTCTCACTCAGAGATGCTGGGCTGCTCCCGGCGTCAAGCTCAACCCGACGCCCCAAACCCTGACCCGAAACCCGAAACCCAAATCATTATCTCTTCCACTCTCAGCTAACTCCACCTGCAGCCTCTTCTCCTTCACTCCTCCCCCCTCACCAACCTTCACCAAATCCTCCTCCTCCGTCCGTACCGCCGTCGCCGCCGTCGAGTCTGACCAGCTCAGCTCCGCCGAAAAGAAACCGAGCAGGTACTACTTTGTTGTGGCCAATGCCAAGTTCATGCTCGATGAGGAGGAGCATTTCCAGGAGCAACTGTTCGAGCGGCTTCGATTGTTTCAAGAGAGGAACAACGAGCAGGACTTTTGGCTTGTGATCGAGCCCAAGTTCTTGGATAAGTTTCCTGACATTACTAGAAGATTAGGCAGGCCTGCTGTGGCTCTGGTCTCCACTAATGGCCCTTGGATTACATTTATGAAGCTGAGATTGGACCGAGTATTGTCAGAAAGTTTTGAAGCTGAGAGTCTAGAAGAAGCATTAGCCTGCAGTCCAACAAATATTGAGTTCAAGAAGCCAGATAAATGGGTGGCACCATACCCCAAGTATGAATCTGAGTGGTGGGGGCCCTTCTTGCCCCCAGGATCGAAAGAGGAGGCCAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

221

Amino Acids

25.12

Weight (kDa)

7.65

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 84 - 173 4.6e-34 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 134
Acc36I ACCTGC 2 cut(s) 134, 240
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 325
AciI CCGC 4 cut(s) 195, 204, 231, 325
AclWI GGATC 1 cut(s) 649
AcoI YGGCCR 1 cut(s) 267
AcyI GRCGYC 2 cut(s) 42, 59
AfaI GTAC 2 cut(s) 193, 254
AgsI TTSAA 3 cut(s) 341, 515, 568
AjnI CCWGG 2 cut(s) 306, 637
AjuI GAANNNNNNNTTGG 4 cut(s) 263, 295, 470, 502
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 6 cut(s) 49, 116, 223, 228, 481, 518
AluI AGCT 6 cut(s) 49, 116, 223, 228, 481, 518
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 1 cut(s) 649
AoxI GGCC 5 cut(s) 267, 428, 457, 623, 654
ApaI GGGCCC 1 cut(s) 627
ApeKI GCWGC 3 cut(s) 5, 32, 129
AspS9I GGNCC 4 cut(s) 458, 490, 623, 624
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 150, 160
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 627
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 384, 627
BbvI GCAGC 3 cut(s) 17, 19, 141
BceAI ACGGC 2 cut(s) 182, 191
BciT130I CCWGG 2 cut(s) 308, 639
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 2 cut(s) 414, 527
BfmI CTRYAG 2 cut(s) 127, 544
BfuAI ACCTGC 2 cut(s) 134, 240
BisI GCNGC 5 cut(s) 6, 33, 130, 204, 326
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 5 cut(s) 7, 34, 131, 205, 327
Bme1390I CCNGG 3 cut(s) 39, 308, 639
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 4 cut(s) 458, 490, 623, 624
BmiI GGNNCC 3 cut(s) 591, 624, 625
BmrFI CCNGG 3 cut(s) 39, 308, 639
BmsI GCATC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 39
BsaHI GRCGYC 2 cut(s) 42, 59
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 2 cut(s) 461, 637
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
BseBI CCWGG 2 cut(s) 308, 639
BseDI CCNNGG 2 cut(s) 461, 637
BseMII CTCAG 6 cut(s) 32, 126, 238, 473, 510, 603
BseRI GAGGAG 6 cut(s) 138, 169, 172, 308, 311, 665
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 3 cut(s) 17, 19, 141
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 5 cut(s) 269, 430, 459, 625, 656
BshNI GGYRCC 1 cut(s) 589
BsiSI CCGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 5 cut(s) 269, 430, 459, 625, 656
Bso31I GGTCTC 1 cut(s) 451
Bsp120I GGGCCC 1 cut(s) 623
Bsp1286I GDGCHC 2 cut(s) 384, 627
Bsp143I GATC 2 cut(s) 375, 641
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 4 cut(s) 195, 204, 231, 325
BspANI GGCC 5 cut(s) 269, 430, 459, 625, 656
BspCNI CTCAG 6 cut(s) 31, 125, 237, 474, 511, 604
BspLI GGNNCC 3 cut(s) 591, 624, 625
BspMAI CTGCAG 2 cut(s) 131, 548
BspMI ACCTGC 2 cut(s) 134, 240
BspPI GGATC 1 cut(s) 649
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 325
BssECI CCNNGG 2 cut(s) 461, 637
BssMI GATC 2 cut(s) 375, 641
BssNI GRCGYC 2 cut(s) 42, 59
BssT1I CCWWGG 1 cut(s) 461
Bst2UI CCWGG 2 cut(s) 308, 639
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 2 cut(s) 108, 140
BstACI GRCGYC 2 cut(s) 42, 59
BstC8I GCNNGC 3 cut(s) 428, 432, 544
BstDEI CTNAG 6 cut(s) 18, 112, 224, 482, 519, 612
BstKTI GATC 2 cut(s) 378, 644
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 2 cut(s) 375, 641
BstMWI GCNNNNNNNGC 2 cut(s) 203, 631
BstNI CCWGG 2 cut(s) 308, 639
BstSCI CCNGG 3 cut(s) 37, 306, 637
BstSFI CTRYAG 2 cut(s) 127, 544
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 3 cut(s) 17, 19, 141
BstXI CCANNNNNNTGG 2 cut(s) 391, 582
BsuRI GGCC 5 cut(s) 269, 430, 459, 625, 656
BveI ACCTGC 2 cut(s) 134, 240
Cac8I GCNNGC 3 cut(s) 428, 432, 544
Cfr13I GGNCC 4 cut(s) 458, 490, 623, 624
CseI GACGC 2 cut(s) 31, 67
Csp6I GTAC 2 cut(s) 192, 253
CviAII CATG 1 cut(s) 283
CviQI GTAC 2 cut(s) 192, 253
DdeI CTNAG 6 cut(s) 18, 112, 224, 482, 519, 612
DpnI GATC 2 cut(s) 377, 643
DpnII GATC 2 cut(s) 375, 641
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 108, 140
EarI CTCTTC 2 cut(s) 108, 140
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 461
Eco147I AGGCCT 1 cut(s) 430
Eco24I GRGCYC 2 cut(s) 384, 627
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 490
EcoO109I RGGNCCY 2 cut(s) 623, 624
EcoRII CCWGG 2 cut(s) 306, 637
EcoT14I CCWWGG 1 cut(s) 461
EcoT38I GRGCYC 2 cut(s) 384, 627
ErhI CCWWGG 1 cut(s) 461
FaeI CATG 1 cut(s) 286
FaiI YATR 4 cut(s) 284, 476, 595, 606
FatI CATG 1 cut(s) 282
Fnu4HI GCNGC 5 cut(s) 6, 33, 130, 204, 326
FriOI GRGCYC 2 cut(s) 384, 627
Fsp4HI GCNGC 5 cut(s) 6, 33, 130, 204, 326
FspBI CTAG 2 cut(s) 414, 527
GluI GCNGC 5 cut(s) 6, 33, 130, 204, 326
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 5 cut(s) 269, 430, 459, 625, 656
HapII CCGG 1 cut(s) 39
HgaI GACGC 2 cut(s) 31, 67
Hin1I GRCGYC 2 cut(s) 42, 59
Hin1II CATG 1 cut(s) 286
HinfI GANTC 3 cut(s) 212, 523, 608
HpaII CCGG 1 cut(s) 39
HphI GGTGA 2 cut(s) 150, 160
Hpy188I TCNGA 4 cut(s) 21, 217, 505, 613
Hpy188III TCNNGA 4 cut(s) 341, 404, 527, 568
Hpy99I CGWCG 3 cut(s) 61, 203, 212
HpyAV CCTTC 3 cut(s) 151, 175, 637
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 129, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 631
HpyF3I CTNAG 6 cut(s) 18, 112, 224, 482, 519, 612
Hsp92I GRCGYC 2 cut(s) 42, 59
Hsp92II CATG 1 cut(s) 286
Kzo9I GATC 2 cut(s) 375, 641
LmnI GCTCC 4 cut(s) 40, 233, 298, 310
Lsp1109I GCAGC 3 cut(s) 17, 19, 141
LweI GCATC 1 cut(s) 14
MaeI CTAG 2 cut(s) 414, 527
MalI GATC 2 cut(s) 377, 643
MbiI CCGCTC 1 cut(s) 325
MboI GATC 2 cut(s) 375, 641
MboII GAAGA 4 cut(s) 95, 127, 429, 542
MhlI GDGCHC 2 cut(s) 384, 627
MlsI TGGCCA 1 cut(s) 269
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 2 cut(s) 221, 532
MmeI TCCRAC 1 cut(s) 575
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 269
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 3 cut(s) 39, 308, 639
MvaI CCWGG 2 cut(s) 308, 639
MwoI GCNNNNNNNGC 2 cut(s) 203, 631
NciI CCSGG 1 cut(s) 39
NdeII GATC 2 cut(s) 375, 641
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 3 cut(s) 591, 624, 625
PaqCI CACCTGC 1 cut(s) 134
PceI AGGCCT 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 1 cut(s) 608
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 5 cut(s) 7, 34, 131, 205, 327
PleI GAGTC 2 cut(s) 220, 531
PpsI GAGTC 2 cut(s) 220, 531
Psp6I CCWGG 2 cut(s) 306, 637
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 2 cut(s) 306, 637
PspN4I GGNNCC 3 cut(s) 591, 624, 625
PspOMI GGGCCC 1 cut(s) 623
PspPI GGNCC 4 cut(s) 458, 490, 623, 624
PstI CTGCAG 2 cut(s) 131, 548
RsaI GTAC 2 cut(s) 193, 254
RsaNI GTAC 2 cut(s) 192, 253
SatI GCNGC 5 cut(s) 6, 33, 130, 204, 326
Sau3AI GATC 2 cut(s) 375, 641
Sau96I GGNCC 4 cut(s) 458, 490, 623, 624
SchI GAGTC 2 cut(s) 221, 532
ScrFI CCNGG 3 cut(s) 39, 308, 639
SduI GDGCHC 2 cut(s) 384, 627
SetI ASST 9 cut(s) 51, 118, 128, 167, 225, 230, 254, 483, 520
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 2 cut(s) 127, 544
SinI GGWCC 1 cut(s) 490
SseBI AGGCCT 1 cut(s) 430
SsiI CCGC 4 cut(s) 195, 204, 231, 325
SspI AATATT 1 cut(s) 559
SspMI CTAG 2 cut(s) 414, 527
StuI AGGCCT 1 cut(s) 430
StyD4I CCNGG 3 cut(s) 37, 306, 637
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 1 cut(s) 318
TaqI TCGA 6 cut(s) 210, 288, 321, 331, 378, 644
TaqII GACCGA 1 cut(s) 507
TauI GCSGC 2 cut(s) 206, 328
TfiI GAWTC 1 cut(s) 608
TseI GCWGC 3 cut(s) 5, 32, 129
TspDTI ATGAA 3 cut(s) 271, 491, 621
TspGWI ACGGA 2 cut(s) 175, 179
VpaK11BI GGWCC 1 cut(s) 490
XbaI TCTAGA 1 cut(s) 526
XspI CTAG 2 cut(s) 414, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.