pycom03g03650

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010918
Physical Location & Seq
Forward (+)
2904508 .. 2905603
1096 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03650.2

Sequence Viewer

Length: 381 bp
ATGACGAGGAGCATTTCCAGGAGCAGTTGTGTGAGAGGCTTCGATACTATGGAGAGAAGAACAAAGAGCAGGACTTTTGGCTTAACCGTGATCGAGCCTAAGTTCTTGGATAAGTTTCCTAACATTACTAAGAGATTAGGAAGGCCTTCCGTGGCTCTGGTTTCGACCAACGGTCCTTGGATTACGTTTATGAAGCTGAGACTGGACAGAGTGTTAGCAGAAAGTTTTGAAGCCGAGAGTCTAGAAGAAGCGTTAGCCTCCAATCCAACCACTATCGAGTTTGAGAAGCCACAGAAATGGGTAGCACCTTATTCCAAGTATGAATCCGGGTGGTGGGGGTCCTTCTTGCCGCCAAGAACGAAAGAGGGGAGCAAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

127

Amino Acids

14.39

Weight (kDa)

9.86

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 1 cut(s) 230
AhdI GACNNNNNGTC 1 cut(s) 171
AjnI CCWGG 1 cut(s) 17
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 193
AoxI GGCC 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 173, 339
AsuC2I CCSGG 1 cut(s) 328
AvaII GGWCC 2 cut(s) 173, 339
BciT130I CCWGG 1 cut(s) 19
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 242
BisI GCNGC 1 cut(s) 350
BlsI GCNGC 1 cut(s) 351
Bme1390I CCNGG 2 cut(s) 19, 328
Bme18I GGWCC 2 cut(s) 173, 339
BmeRI GACNNNNNGTC 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 173, 339
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 19, 328
BpuMI CCSGG 1 cut(s) 328
BsaJI CCNNGG 2 cut(s) 150, 176
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 1 cut(s) 207
BseBI CCWGG 1 cut(s) 19
BseDI CCNNGG 2 cut(s) 150, 176
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 22
BshFI GGCC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 1 cut(s) 193
BsnI GGCC 1 cut(s) 145
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 1 cut(s) 350
BspANI GGCC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 340
BsrI ACTGG 1 cut(s) 207
BssECI CCNNGG 2 cut(s) 150, 176
BssMI GATC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 2 cut(s) 88, 173
BstDEI CTNAG 3 cut(s) 99, 129, 197
BstDSI CCRYGG 1 cut(s) 150
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 19
BstSCI CCNGG 2 cut(s) 17, 326
BstXI CCANNNNNNTGG 1 cut(s) 297
BsuRI GGCC 1 cut(s) 145
BtgI CCRYGG 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 173, 339
CviJI RGCY 9 cut(s) 39, 81, 97, 145, 155, 196, 233, 257, 289
CviKI_1 RGCY 9 cut(s) 39, 81, 97, 145, 155, 196, 233, 257, 289
DdeI CTNAG 3 cut(s) 99, 129, 197
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
DriI GACNNNNNGTC 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 176
Eco147I AGGCCT 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 173, 339
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaiI YATR 3 cut(s) 50, 191, 321
Fnu4HI GCNGC 1 cut(s) 350
Fsp4HI GCNGC 1 cut(s) 350
FspBI CTAG 1 cut(s) 242
GluI GCNGC 1 cut(s) 350
HaeIII GGCC 1 cut(s) 145
HapII CCGG 1 cut(s) 327
HinfI GANTC 2 cut(s) 238, 323
HpaII CCGG 1 cut(s) 327
Hpy188III TCNNGA 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 135, 156, 352
HpyCH4III ACNGT 2 cut(s) 88, 173
HpyCH4IV ACGT 1 cut(s) 185
HpyF3I CTNAG 3 cut(s) 99, 129, 197
HpySE526I ACGT 1 cut(s) 185
Kzo9I GATC 1 cut(s) 90
LmnI GCTCC 3 cut(s) 9, 21, 369
LpnPI CCDG 6 cut(s) 4, 31, 55, 143, 188, 340
MaeI CTAG 1 cut(s) 242
MaeII ACGT 1 cut(s) 185
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 2 cut(s) 69, 257
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 3 cut(s) 29, 268, 358
MroXI GAANNNNTTC 1 cut(s) 145
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 295
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 19, 328
MvaI CCWGG 1 cut(s) 19
NciI CCSGG 1 cut(s) 328
NdeII GATC 1 cut(s) 90
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 259
PceI AGGCCT 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 145
PfeI GAWTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 17
PkrI GCNGC 1 cut(s) 351
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
PpuMI RGGWCCY 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 17
PspGI CCWGG 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 2 cut(s) 173, 339
PspPPI RGGWCCY 1 cut(s) 339
RseI CAYNNNNRTG 1 cut(s) 295
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 1 cut(s) 350
Sau3AI GATC 1 cut(s) 90
Sau96I GGNCC 2 cut(s) 173, 339
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 19, 328
SetI ASST 3 cut(s) 188, 198, 310
SinI GGWCC 2 cut(s) 173, 339
SmiMI CAYNNNNRTG 1 cut(s) 295
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 1 cut(s) 350
SspMI CTAG 1 cut(s) 242
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 17, 326
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 2 cut(s) 88, 173
TaiI ACGT 1 cut(s) 188
TaqI TCGA 4 cut(s) 42, 93, 164, 276
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 206, 336
TspGWI ACGGA 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 173, 339
XbaI TCTAGA 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 145
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.