MD11G1049300.v1.1

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010918
Physical Location & Seq
Forward (+)
4232010 .. 4233815
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1049300.v1.1.491

Sequence Viewer

Length: 687 bp
ATGTCAGCCGTGGCAACTCTCGGTCAGGCATGCTGGGCCGCCGCCGCCGTGAAATCGAAACCAACCACGGCCATCTCACAAACCCACATCCGAAATCCAAAGCCCAAATCTTTATCTCTTCCTCTCTCAGCCAATTCCAGCTGCAACCACTTCTTCTCCTTCCCTCCCAAAACCACCGTCAAATCCTCCTCCTCCGTCCGTACATCTGTCGCCGCCGTCGACTCCGACCAGCTCAGCTCCTCCGATTCCGCCGATAAGGAAAAGCCGAGCAGGTACTATTTCGTGGTTGCGAATGCGAAGTTTATGCTCGATGAGGAGGAGCATTTCCAGGAGCAATTGTGTGAGAGGCTTCGACACTATGGAGAGAAGAACAAAGAGCAGGACTTTTGGCTTGTGATCGAGCCTAAGTTCTTGGATAAGTTTCCTAACATTACTAAGAGATTAGGAAGGCCTGCCGTTGCTCTGGTTTCGACTAACGGACCTTGGATTACGTTTATGAAGCTGAGACTGGACAGAGTGTTAGCAGAAAGTTTTGAAGCTGCGAGTCTAGAAGAAGCATTAGCCTCAAATCCAACCAAGATCGAGTTTGAGAAGCCGCAGAAATGGGTAGCACCTTATTCCAAGTATGAATCCGGGTGGTGGGGACCCTTCTTGCCTCCAGGATCGAAAGAGGAGGCCAAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

229

Amino Acids

25.49

Weight (kDa)

8.86

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 91 - 180 9.2e-36 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 219
AciI CCGC 6 cut(s) 39, 42, 45, 213, 249, 596
AclWI GGATC 1 cut(s) 670
AcoI YGGCCR 1 cut(s) 69
AfaI GTAC 2 cut(s) 202, 275
AfiI CCNNNNNNNGG 1 cut(s) 639
AgsI TTSAA 1 cut(s) 536
AjnI CCWGG 2 cut(s) 327, 658
AluBI AGCT 5 cut(s) 141, 232, 237, 502, 539
AluI AGCT 5 cut(s) 141, 232, 237, 502, 539
Alw26I GTCTC 1 cut(s) 499
AlwI GGATC 1 cut(s) 670
AoxI GGCC 4 cut(s) 36, 69, 449, 675
ApeKI GCWGC 2 cut(s) 141, 539
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspS9I GGNCC 3 cut(s) 36, 479, 644
AsuC2I CCSGG 1 cut(s) 634
AvaII GGWCC 2 cut(s) 479, 644
BbvI GCAGC 2 cut(s) 128, 526
BccI CCATC 1 cut(s) 80
BceAI ACGGC 4 cut(s) 32, 84, 200, 440
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 2 cut(s) 329, 660
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 499
BfaI CTAG 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 261
BisI GCNGC 7 cut(s) 39, 42, 45, 142, 213, 540, 596
BlpI GCTNAGC 1 cut(s) 233
BlsI GCNGC 7 cut(s) 40, 43, 46, 143, 214, 541, 597
Bme1390I CCNGG 3 cut(s) 329, 634, 660
Bme18I GGWCC 2 cut(s) 479, 644
BmgT120I GGNCC 3 cut(s) 36, 479, 644
BmiI GGNNCC 2 cut(s) 645, 646
BmrFI CCNGG 3 cut(s) 329, 634, 660
BpmI CTGGAG 1 cut(s) 642
Bpu1102I GCTNAGC 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 634
BsaJI CCNNGG 3 cut(s) 9, 66, 482
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 323, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse1I ACTGG 1 cut(s) 513
BseBI CCWGG 2 cut(s) 329, 660
BseDI CCNNGG 3 cut(s) 9, 66, 482
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMII CTCAG 3 cut(s) 141, 247, 494
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 6 cut(s) 178, 181, 229, 329, 332, 686
BseXI GCAGC 2 cut(s) 128, 526
BseYI CCCAGC 1 cut(s) 33
BshFI GGCC 4 cut(s) 38, 71, 451, 677
BsiSI CCGG 1 cut(s) 633
BslFI GGGAC 1 cut(s) 657
BslI CCNNNNNNNGG 1 cut(s) 639
BsmAI GTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 657
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 4 cut(s) 38, 71, 451, 677
Bsp143I GATC 3 cut(s) 396, 579, 662
Bsp1720I GCTNAGC 1 cut(s) 233
BspACI CCGC 6 cut(s) 39, 42, 45, 213, 249, 596
BspANI GGCC 4 cut(s) 38, 71, 451, 677
BspCNI CTCAG 3 cut(s) 140, 246, 495
BspLI GGNNCC 2 cut(s) 645, 646
BspMI ACCTGC 1 cut(s) 261
BspPI GGATC 1 cut(s) 670
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 3 cut(s) 9, 66, 482
BssMI GATC 3 cut(s) 396, 579, 662
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 329, 660
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 31, 453
BstDEI CTNAG 5 cut(s) 127, 233, 405, 435, 503
BstDSI CCRYGG 2 cut(s) 9, 66
BstF5I GGATG 1 cut(s) 87
BstKTI GATC 3 cut(s) 399, 582, 665
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 3 cut(s) 396, 579, 662
BstMWI GCNNNNNNNGC 2 cut(s) 35, 44
BstNI CCWGG 2 cut(s) 329, 660
BstNSI RCATGY 1 cut(s) 33
BstSCI CCNGG 3 cut(s) 327, 632, 658
BstV1I GCAGC 2 cut(s) 128, 526
BsuRI GGCC 4 cut(s) 38, 71, 451, 677
BtgI CCRYGG 2 cut(s) 9, 66
BtsCI GGATG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 2 cut(s) 31, 453
Cfr13I GGNCC 3 cut(s) 36, 479, 644
Csp6I GTAC 2 cut(s) 201, 274
CviAII CATG 1 cut(s) 30
CviQI GTAC 2 cut(s) 201, 274
DdeI CTNAG 5 cut(s) 127, 233, 405, 435, 503
DpnI GATC 3 cut(s) 398, 581, 664
DpnII GATC 3 cut(s) 396, 579, 662
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 482
Eco147I AGGCCT 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 479, 644
EcoO109I RGGNCCY 1 cut(s) 644
EcoRII CCWGG 2 cut(s) 327, 658
EcoT14I CCWWGG 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 1 cut(s) 33
FaiI YATR 5 cut(s) 31, 305, 360, 497, 627
FaqI GGGAC 1 cut(s) 657
FatI CATG 1 cut(s) 29
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 7 cut(s) 39, 42, 45, 142, 213, 540, 596
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 7 cut(s) 39, 42, 45, 142, 213, 540, 596
FspBI CTAG 1 cut(s) 548
GluI GCNGC 7 cut(s) 39, 42, 45, 142, 213, 540, 596
GsaI CCCAGC 1 cut(s) 37
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 4 cut(s) 38, 71, 451, 677
HapII CCGG 1 cut(s) 633
Hin1II CATG 1 cut(s) 33
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 4 cut(s) 221, 245, 544, 629
HpaII CCGG 1 cut(s) 633
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 3 cut(s) 92, 226, 244
Hpy188III TCNNGA 1 cut(s) 548
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 169, 441, 658
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 44
HpyF3I CTNAG 5 cut(s) 127, 233, 405, 435, 503
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 1 cut(s) 33
KflI GGGWCCC 1 cut(s) 644
Kzo9I GATC 3 cut(s) 396, 579, 662
LmnI GCTCC 3 cut(s) 242, 319, 331
Lsp1109I GCAGC 2 cut(s) 128, 526
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 491
MalI GATC 3 cut(s) 398, 581, 664
MboI GATC 3 cut(s) 396, 579, 662
MboII GAAGA 4 cut(s) 110, 145, 379, 563
MfeI CAATTG 1 cut(s) 335
MluCI AATT 2 cut(s) 133, 335
MlyI GAGTC 2 cut(s) 215, 553
MmeI TCCRAC 2 cut(s) 249, 596
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 3 cut(s) 329, 634, 660
MunI CAATTG 1 cut(s) 335
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 2 cut(s) 329, 660
MwoI GCNNNNNNNGC 2 cut(s) 35, 44
NciI CCSGG 1 cut(s) 634
NdeII GATC 3 cut(s) 396, 579, 662
NlaIII CATG 1 cut(s) 33
NlaIV GGNNCC 2 cut(s) 645, 646
NmeAIII GCCGAG 1 cut(s) 291
NspI RCATGY 1 cut(s) 33
PaeI GCATGC 1 cut(s) 33
PceI AGGCCT 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 245, 629
PfoI TCCNGGA 2 cut(s) 327, 658
PkrI GCNGC 7 cut(s) 40, 43, 46, 143, 214, 541, 597
PleI GAGTC 2 cut(s) 215, 552
PpsI GAGTC 2 cut(s) 215, 552
PpuMI RGGWCCY 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 2 cut(s) 327, 658
PspFI CCCAGC 1 cut(s) 33
PspGI CCWGG 2 cut(s) 327, 658
PspN4I GGNNCC 2 cut(s) 645, 646
PspPI GGNCC 3 cut(s) 36, 479, 644
PspPPI RGGWCCY 1 cut(s) 644
PvuII CAGCTG 1 cut(s) 141
RsaI GTAC 2 cut(s) 202, 275
RsaNI GTAC 2 cut(s) 201, 274
SalI GTCGAC 1 cut(s) 218
SatI GCNGC 7 cut(s) 39, 42, 45, 142, 213, 540, 596
Sau3AI GATC 3 cut(s) 396, 579, 662
Sau96I GGNCC 3 cut(s) 36, 479, 644
SchI GAGTC 2 cut(s) 215, 553
ScrFI CCNGG 3 cut(s) 329, 634, 660
SetI ASST 9 cut(s) 143, 234, 239, 275, 484, 494, 504, 541, 616
SinI GGWCC 2 cut(s) 479, 644
SphI GCATGC 1 cut(s) 33
Sse9I AATT 2 cut(s) 133, 335
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 6 cut(s) 39, 42, 45, 213, 249, 596
SspMI CTAG 1 cut(s) 548
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 3 cut(s) 327, 632, 658
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 494
TaqI TCGA 8 cut(s) 56, 219, 309, 352, 399, 470, 582, 665
TaqII GACCGA 1 cut(s) 11
TasI AATT 2 cut(s) 133, 335
TauI GCSGC 5 cut(s) 41, 44, 47, 215, 598
TfiI GAWTC 2 cut(s) 245, 629
TseI GCWGC 2 cut(s) 141, 539
TspDTI ATGAA 2 cut(s) 512, 642
TspGWI ACGGA 3 cut(s) 184, 188, 492
VpaK11BI GGWCC 2 cut(s) 479, 644
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.