FvH4_3g41200

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010918
Physical Location & Seq
Forward (+)
34660487 .. 34662909
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41200.t1

Sequence Viewer

Length: 648 bp
ATGGCAGCGACACTCTCTCAGCGATGCTGGGCTGCTCCCGGCGTCAAGCTCAACCCGACCCGAAACCCGAAACCCAAATCACTCTCTCTTCCCCTCTCAGCCAACTCCACCTGCACCCTCTTCTCCTTCTCTCCTCCCTTCCCCAAACTCTCCTCCTCCTCCGTCCGTACCGCCGTCGCCGCCGTCGAGTCCGACCAGCTCAGCAGCTCCTCTACCGAAAAGAAACCGAGCAAGTACTACTTTGTTGTGGCGAATGCCAAGTTCATGCTGGACGAGGAAGAGCATTTCCAGGAACAGCTGTTCGAGCGGCTTCGGTTGTTTAAGGAGAGGAACAATGAGCAGGACTTTTGGCTTGTGATCGAGCCTAAGTTTTTGGATAAGTTTCCCGACATTACTAGAAGACTAGGCAGGCCTGCTGTGGCTCTTGTCTCCACTAATGGCCCTTGGATTACGTTTATGAAGCTGAGATTGGACCGAGTATTATCAGAAAGTTTTGAAGCTGATAGTCTAGAAGAAGCATTAGCCTGCAGTCCAACAAAAATTGAGTTCAAGAAGCCAGATAAATGGGTAGCACCATACCCCAAGTATGAATCAGAGTGGTGGGGGCCCTTCTTGCCCCCAAGATCGAAGAAGGAGGCCAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

216

Amino Acids

24.57

Weight (kDa)

9.18

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 78 - 167 3.4e-34 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 307
AciI CCGC 3 cut(s) 171, 180, 307
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 2 cut(s) 169, 236
AgsI TTSAA 2 cut(s) 497, 550
AjnI CCWGG 1 cut(s) 288
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AloI GAACNNNNNNTCC 2 cut(s) 284, 316
AluBI AGCT 6 cut(s) 49, 199, 207, 298, 463, 500
AluI AGCT 6 cut(s) 49, 199, 207, 298, 463, 500
Alw26I GTCTC 1 cut(s) 433
AoxI GGCC 4 cut(s) 410, 439, 605, 636
ApaI GGGCCC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 5, 32, 204
AspS9I GGNCC 4 cut(s) 440, 472, 605, 606
AsuC2I CCSGG 1 cut(s) 39
AvaII GGWCC 1 cut(s) 472
BaeGI GKGCMC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 406
BbvI GCAGC 3 cut(s) 17, 19, 216
BceAI ACGGC 2 cut(s) 158, 167
BciT130I CCWGG 1 cut(s) 290
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 433
BfaI CTAG 3 cut(s) 396, 404, 509
BfmI CTRYAG 1 cut(s) 526
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 5 cut(s) 6, 33, 180, 205, 308
BlpI GCTNAGC 1 cut(s) 200
BlsI GCNGC 5 cut(s) 7, 34, 181, 206, 309
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 2 cut(s) 39, 290
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 4 cut(s) 440, 472, 605, 606
BmiI GGNNCC 2 cut(s) 606, 607
BmrFI CCNGG 2 cut(s) 39, 290
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 406
Bpu1102I GCTNAGC 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 443
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 443
BseMII CTCAG 4 cut(s) 32, 111, 214, 455
BseRI GAGGAG 5 cut(s) 123, 142, 145, 148, 199
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 3 cut(s) 17, 19, 216
BseYI CCCAGC 1 cut(s) 27
BsgI GTGCAG 1 cut(s) 97
BshFI GGCC 4 cut(s) 412, 441, 607, 638
BsiSI CCGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 433
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 4 cut(s) 412, 441, 607, 638
Bsp120I GGGCCC 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 2 cut(s) 357, 623
Bsp1720I GCTNAGC 1 cut(s) 200
BspACI CCGC 3 cut(s) 171, 180, 307
BspANI GGCC 4 cut(s) 412, 441, 607, 638
BspCNI CTCAG 4 cut(s) 31, 110, 213, 456
BspLI GGNNCC 2 cut(s) 606, 607
BspMAI CTGCAG 1 cut(s) 530
BspMI ACCTGC 1 cut(s) 119
BspQI GCTCTTC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 443
BssMI GATC 2 cut(s) 357, 623
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 290
Bst6I CTCTTC 3 cut(s) 93, 125, 273
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 3 cut(s) 410, 414, 526
BstDEI CTNAG 5 cut(s) 18, 97, 200, 366, 464
BstKTI GATC 2 cut(s) 360, 626
BstMAI GTCTC 1 cut(s) 433
BstMBI GATC 2 cut(s) 357, 623
BstMWI GCNNNNNNNGC 3 cut(s) 179, 304, 613
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 37, 288
BstSFI CTRYAG 1 cut(s) 526
BstSLI GKGCMC 1 cut(s) 609
BstV1I GCAGC 3 cut(s) 17, 19, 216
BstV2I GAAGAC 1 cut(s) 406
BstXI CCANNNNNNTGG 1 cut(s) 564
BsuRI GGCC 4 cut(s) 412, 441, 607, 638
BtgZI GCGATG 1 cut(s) 37
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 3 cut(s) 410, 414, 526
Cfr13I GGNCC 4 cut(s) 440, 472, 605, 606
CseI GACGC 1 cut(s) 31
Csp6I GTAC 2 cut(s) 168, 235
CviAII CATG 1 cut(s) 265
CviQI GTAC 2 cut(s) 168, 235
DdeI CTNAG 5 cut(s) 18, 97, 200, 366, 464
DpnI GATC 2 cut(s) 359, 625
DpnII GATC 2 cut(s) 357, 623
Eam1104I CTCTTC 3 cut(s) 93, 125, 273
EarI CTCTTC 3 cut(s) 93, 125, 273
Eco130I CCWWGG 1 cut(s) 443
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 609
Eco47I GGWCC 1 cut(s) 472
EcoO109I RGGNCCY 2 cut(s) 605, 606
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 1 cut(s) 268
FaiI YATR 4 cut(s) 266, 458, 577, 588
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 1 cut(s) 264
Fnu4HI GCNGC 5 cut(s) 6, 33, 180, 205, 308
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 5 cut(s) 6, 33, 180, 205, 308
FspBI CTAG 3 cut(s) 396, 404, 509
GluI GCNGC 5 cut(s) 6, 33, 180, 205, 308
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 4 cut(s) 412, 441, 607, 638
HapII CCGG 1 cut(s) 39
HgaI GACGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 1 cut(s) 268
HinfI GANTC 2 cut(s) 188, 590
HpaII CCGG 1 cut(s) 39
Hpy188I TCNGA 3 cut(s) 193, 487, 595
Hpy188III TCNNGA 3 cut(s) 386, 509, 550
Hpy99I CGWCG 2 cut(s) 179, 188
HpyAV CCTTC 4 cut(s) 136, 148, 619, 625
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 2 cut(s) 114, 528
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 304, 613
HpyF3I CTNAG 5 cut(s) 18, 97, 200, 366, 464
HpySE526I ACGT 1 cut(s) 452
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 1 cut(s) 268
Kzo9I GATC 2 cut(s) 357, 623
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 40, 212
Lsp1109I GCAGC 3 cut(s) 17, 19, 216
LweI GCATC 1 cut(s) 14
MaeI CTAG 3 cut(s) 396, 404, 509
MaeII ACGT 1 cut(s) 452
MalI GATC 2 cut(s) 359, 625
MbiI CCGCTC 1 cut(s) 307
MboI GATC 2 cut(s) 357, 623
MboII GAAGA 6 cut(s) 80, 112, 290, 411, 524, 640
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 1 cut(s) 540
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 216, 557
MseI TTAA 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 2 cut(s) 39, 290
Mva1269I GAATGC 1 cut(s) 259
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 179, 304, 613
NciI CCSGG 1 cut(s) 39
NdeII GATC 2 cut(s) 357, 623
NlaIII CATG 1 cut(s) 268
NlaIV GGNNCC 2 cut(s) 606, 607
PaqCI CACCTGC 1 cut(s) 119
PceI AGGCCT 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 259
PfeI GAWTC 1 cut(s) 590
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 5 cut(s) 7, 34, 181, 206, 309
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 288
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 606, 607
PspOMI GGGCCC 1 cut(s) 605
PspPI GGNCC 4 cut(s) 440, 472, 605, 606
PstI CTGCAG 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 298
RsaI GTAC 2 cut(s) 169, 236
RsaNI GTAC 2 cut(s) 168, 235
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 5 cut(s) 6, 33, 180, 205, 308
Sau3AI GATC 2 cut(s) 357, 623
Sau96I GGNCC 4 cut(s) 440, 472, 605, 606
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 39, 290
SduI GDGCHC 1 cut(s) 609
SetI ASST 8 cut(s) 51, 113, 201, 209, 300, 455, 465, 502
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 526
SinI GGWCC 1 cut(s) 472
Sse9I AATT 1 cut(s) 540
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 3 cut(s) 171, 180, 307
SspMI CTAG 3 cut(s) 396, 404, 509
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 2 cut(s) 37, 288
StyI CCWWGG 1 cut(s) 443
TaiI ACGT 1 cut(s) 455
TaqI TCGA 4 cut(s) 186, 303, 360, 626
TaqII GACCGA 1 cut(s) 489
TasI AATT 1 cut(s) 540
TatI WGTACW 1 cut(s) 234
TauI GCSGC 2 cut(s) 182, 310
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseI GCWGC 3 cut(s) 5, 32, 204
TspDTI ATGAA 3 cut(s) 253, 473, 603
TspGWI ACGGA 2 cut(s) 151, 155
VpaK11BI GGWCC 1 cut(s) 472
XbaI TCTAGA 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XspI CTAG 3 cut(s) 396, 404, 509
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.