Rh5AG485600

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010918
Physical Location & Seq
Reverse (-)
82976694 .. 82978119
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG485600.1

Sequence Viewer

Length: 663 bp
ATGGCAGCAACTCTCACTCAGAGATGCTGGGCTGCTCCCGGCGTCAAGCTCAACCCGACGCCCCAAACCCTGACCCGAAATCCGAAACCCAAATCATTATCTCTTCCACTCTCAGCTAACTCCACCTGCAGCCTTTTCTCCTTCACTCCTCCTCCCTCACCAACCTTCACCAAAGCCTCCTCAGTCCGTACCGCCGTCGCCGCCGTCGAGTCTGACCAGCTCAGCTCCGCCGAAAAGAAACCGAGCAGGTACTACTTTGTTGTGGCCAATGCCAAGTTCATGCTCGATGAGGAGGAGCATTTCCAGGAGCAACTGTTCGAGCGGCTTCGATTGTTTAAAGAGAGGAACAACGAGCAGGACTTTTGGCTTGTGATCGAGCCCAAGTTCTTGGATAAGTTTCCTGACATTACTAGAAGATTAGGCAGGCCTGCTGTGGCTCTGGTCTCCACTAATGGCCCTTGGATTACATTTATGAAGCTGAGATTGGACCGAGTATTGTCAGAAAGTTTTGAAGCTGAGAGTCTAGAACAAGCATTAGCCTCCAGTCCAACAAATATTGAGTTCAAGAAGCCAGATAAATGGGTAGCACCATACCCCAAGTATGAATCTGAGTGGTGGGGGCCCTTCTTGCCCCCAGGATCGAAAGAGGAGGCCAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

220

Amino Acids

25.0

Weight (kDa)

8.8

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 83 - 172 4.5e-34 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 134
Acc36I ACCTGC 2 cut(s) 134, 237
AccBSI CCGCTC 1 cut(s) 322
AciI CCGC 4 cut(s) 192, 201, 228, 322
AclWI GGATC 1 cut(s) 646
AcoI YGGCCR 1 cut(s) 264
AcyI GRCGYC 2 cut(s) 42, 59
AfaI GTAC 2 cut(s) 190, 251
AgsI TTSAA 2 cut(s) 512, 565
AjnI CCWGG 2 cut(s) 303, 634
AjuI GAANNNNNNNTTGG 4 cut(s) 260, 292, 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 6 cut(s) 49, 116, 220, 225, 478, 515
AluI AGCT 6 cut(s) 49, 116, 220, 225, 478, 515
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 1 cut(s) 646
AoxI GGCC 5 cut(s) 264, 425, 454, 620, 651
ApaI GGGCCC 1 cut(s) 624
ApeKI GCWGC 3 cut(s) 5, 32, 129
AspS9I GGNCC 4 cut(s) 455, 487, 620, 621
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 150, 160
AvaII GGWCC 1 cut(s) 487
BaeGI GKGCMC 1 cut(s) 624
BalI TGGCCA 1 cut(s) 266
BanII GRGCYC 2 cut(s) 381, 624
BbvI GCAGC 3 cut(s) 17, 19, 141
BceAI ACGGC 2 cut(s) 179, 188
BciT130I CCWGG 2 cut(s) 305, 636
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 448
BfaI CTAG 2 cut(s) 411, 524
BfmI CTRYAG 1 cut(s) 127
BfuAI ACCTGC 2 cut(s) 134, 237
BisI GCNGC 5 cut(s) 6, 33, 130, 201, 323
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 5 cut(s) 7, 34, 131, 202, 324
Bme1390I CCNGG 3 cut(s) 39, 305, 636
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 4 cut(s) 455, 487, 620, 621
BmiI GGNNCC 2 cut(s) 621, 622
BmrFI CCNGG 3 cut(s) 39, 305, 636
BmsI GCATC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 526
Bpu1102I GCTNAGC 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 39
BsaHI GRCGYC 2 cut(s) 42, 59
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 2 cut(s) 458, 634
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 299, 329
Bse1I ACTGG 1 cut(s) 543
BseBI CCWGG 2 cut(s) 305, 636
BseDI CCNNGG 2 cut(s) 458, 634
BseMII CTCAG 7 cut(s) 32, 126, 195, 235, 470, 507, 600
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 6 cut(s) 138, 141, 169, 305, 308, 662
BseSI GKGCMC 1 cut(s) 624
BseXI GCAGC 3 cut(s) 17, 19, 141
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 5 cut(s) 266, 427, 456, 622, 653
BsiSI CCGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 448
BsnI GGCC 5 cut(s) 266, 427, 456, 622, 653
Bso31I GGTCTC 1 cut(s) 448
Bsp120I GGGCCC 1 cut(s) 620
Bsp1286I GDGCHC 2 cut(s) 381, 624
Bsp143I GATC 2 cut(s) 372, 638
Bsp1720I GCTNAGC 1 cut(s) 221
BspACI CCGC 4 cut(s) 192, 201, 228, 322
BspANI GGCC 5 cut(s) 266, 427, 456, 622, 653
BspCNI CTCAG 7 cut(s) 31, 125, 194, 234, 471, 508, 601
BspLI GGNNCC 2 cut(s) 621, 622
BspMAI CTGCAG 1 cut(s) 131
BspMI ACCTGC 2 cut(s) 134, 237
BspPI GGATC 1 cut(s) 646
BspTNI GGTCTC 1 cut(s) 448
BsrBI CCGCTC 1 cut(s) 322
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 2 cut(s) 458, 634
BssMI GATC 2 cut(s) 372, 638
BssNI GRCGYC 2 cut(s) 42, 59
BssT1I CCWWGG 1 cut(s) 458
Bst2UI CCWGG 2 cut(s) 305, 636
Bst4CI ACNGT 1 cut(s) 315
Bst6I CTCTTC 1 cut(s) 108
BstACI GRCGYC 2 cut(s) 42, 59
BstC8I GCNNGC 2 cut(s) 425, 429
BstDEI CTNAG 7 cut(s) 18, 112, 181, 221, 479, 516, 609
BstKTI GATC 2 cut(s) 375, 641
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 2 cut(s) 372, 638
BstMWI GCNNNNNNNGC 2 cut(s) 200, 628
BstNI CCWGG 2 cut(s) 305, 636
BstSCI CCNGG 3 cut(s) 37, 303, 634
BstSFI CTRYAG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 624
BstV1I GCAGC 3 cut(s) 17, 19, 141
BstXI CCANNNNNNTGG 2 cut(s) 388, 579
BsuRI GGCC 5 cut(s) 266, 427, 456, 622, 653
BveI ACCTGC 2 cut(s) 134, 237
Cac8I GCNNGC 2 cut(s) 425, 429
Cfr13I GGNCC 4 cut(s) 455, 487, 620, 621
CseI GACGC 2 cut(s) 31, 67
Csp6I GTAC 2 cut(s) 189, 250
CviAII CATG 1 cut(s) 280
CviQI GTAC 2 cut(s) 189, 250
DdeI CTNAG 7 cut(s) 18, 112, 181, 221, 479, 516, 609
DpnI GATC 2 cut(s) 374, 640
DpnII GATC 2 cut(s) 372, 638
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 458
Eco147I AGGCCT 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 381, 624
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 487
EcoO109I RGGNCCY 2 cut(s) 620, 621
EcoRII CCWGG 2 cut(s) 303, 634
EcoT14I CCWWGG 1 cut(s) 458
EcoT38I GRGCYC 2 cut(s) 381, 624
ErhI CCWWGG 1 cut(s) 458
FaeI CATG 1 cut(s) 283
FaiI YATR 4 cut(s) 281, 473, 592, 603
FatI CATG 1 cut(s) 279
Fnu4HI GCNGC 5 cut(s) 6, 33, 130, 201, 323
FriOI GRGCYC 2 cut(s) 381, 624
Fsp4HI GCNGC 5 cut(s) 6, 33, 130, 201, 323
FspBI CTAG 2 cut(s) 411, 524
GluI GCNGC 5 cut(s) 6, 33, 130, 201, 323
GsaI CCCAGC 1 cut(s) 31
GsuI CTGGAG 1 cut(s) 526
HaeIII GGCC 5 cut(s) 266, 427, 456, 622, 653
HapII CCGG 1 cut(s) 39
HgaI GACGC 2 cut(s) 31, 67
Hin1I GRCGYC 2 cut(s) 42, 59
Hin1II CATG 1 cut(s) 283
HinfI GANTC 3 cut(s) 209, 520, 605
HpaII CCGG 1 cut(s) 39
HphI GGTGA 2 cut(s) 150, 160
Hpy188I TCNGA 5 cut(s) 21, 84, 214, 502, 610
Hpy188III TCNNGA 3 cut(s) 401, 524, 565
Hpy99I CGWCG 3 cut(s) 61, 200, 209
HpyAV CCTTC 3 cut(s) 151, 175, 634
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4V TGCA 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 628
HpyF3I CTNAG 7 cut(s) 18, 112, 181, 221, 479, 516, 609
Hsp92I GRCGYC 2 cut(s) 42, 59
Hsp92II CATG 1 cut(s) 283
Kzo9I GATC 2 cut(s) 372, 638
LmnI GCTCC 4 cut(s) 40, 230, 295, 307
Lsp1109I GCAGC 3 cut(s) 17, 19, 141
LweI GCATC 1 cut(s) 14
MaeI CTAG 2 cut(s) 411, 524
MalI GATC 2 cut(s) 374, 640
MbiI CCGCTC 1 cut(s) 322
MboI GATC 2 cut(s) 372, 638
MboII GAAGA 2 cut(s) 95, 426
MhlI GDGCHC 2 cut(s) 381, 624
MlsI TGGCCA 1 cut(s) 266
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 2 cut(s) 218, 529
MmeI TCCRAC 1 cut(s) 572
Mox20I TGGCCA 1 cut(s) 266
MscI TGGCCA 1 cut(s) 266
MseI TTAA 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 266
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 3 cut(s) 39, 305, 636
MvaI CCWGG 2 cut(s) 305, 636
MwoI GCNNNNNNNGC 2 cut(s) 200, 628
NciI CCSGG 1 cut(s) 39
NdeII GATC 2 cut(s) 372, 638
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 2 cut(s) 621, 622
PaqCI CACCTGC 1 cut(s) 134
PceI AGGCCT 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 1 cut(s) 605
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 5 cut(s) 7, 34, 131, 202, 324
PleI GAGTC 2 cut(s) 217, 528
PpsI GAGTC 2 cut(s) 217, 528
Psp6I CCWGG 2 cut(s) 303, 634
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 2 cut(s) 303, 634
PspN4I GGNNCC 2 cut(s) 621, 622
PspOMI GGGCCC 1 cut(s) 620
PspPI GGNCC 4 cut(s) 455, 487, 620, 621
PstI CTGCAG 1 cut(s) 131
RsaI GTAC 2 cut(s) 190, 251
RsaNI GTAC 2 cut(s) 189, 250
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 5 cut(s) 6, 33, 130, 201, 323
Sau3AI GATC 2 cut(s) 372, 638
Sau96I GGNCC 4 cut(s) 455, 487, 620, 621
SchI GAGTC 2 cut(s) 218, 529
ScrFI CCNGG 3 cut(s) 39, 305, 636
SduI GDGCHC 2 cut(s) 381, 624
SetI ASST 9 cut(s) 51, 118, 128, 167, 222, 227, 251, 480, 517
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 127
SinI GGWCC 1 cut(s) 487
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 4 cut(s) 192, 201, 228, 322
SspI AATATT 1 cut(s) 556
SspMI CTAG 2 cut(s) 411, 524
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 3 cut(s) 37, 303, 634
StyI CCWWGG 1 cut(s) 458
TaaI ACNGT 1 cut(s) 315
TaqI TCGA 6 cut(s) 207, 285, 318, 328, 375, 641
TaqII GACCGA 1 cut(s) 504
TauI GCSGC 2 cut(s) 203, 325
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseI GCWGC 3 cut(s) 5, 32, 129
TspDTI ATGAA 3 cut(s) 268, 488, 618
TspGWI ACGGA 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 487
XbaI TCTAGA 1 cut(s) 523
XspI CTAG 2 cut(s) 411, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.