MD03G1046500.v1.1

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010918
Physical Location & Seq
Forward (+)
3729312 .. 3731122
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046500.v1.1.491

Sequence Viewer

Length: 681 bp
ATGTCAGCCGTGACAACTCTCAGTCAGGCATGCTGGGCTGCCGCCGTCGTGAAACCGAAATCAAACACCGCCATTTCCCATATCCAAAACCCAAAGCCCAAATCTTTAGCTCTTCCTTTCTCGGCCAATTCCAGCTGCGGTCACTTCTTCTCCTTCAATCCCAAAACCACCCTCAAATCCTCCTCCTCCGTCCGTACATTCGTTGCCGCCGTTGAATCCGACCAGCTCAGCTCCTCCGACTCCGCTGACAAGGAAAAGCCGAGCAGGTACTATTTCGTGGTTGCAAATGCGAAGTTTATGCTCGATGACGAGGAGCATTTCCAGGAGCAGTTGTGTGAGAGGCTTCGATACTATGGAGAGAAGAACAAAGAGCAGGACTTTTGGCTTGTGATCGAGCCTAAGTTCTTGGATAAGTTTCCTAACATTACTAAGAGATTAGGAAGGCCTGCCGTGGCTCTGGTTTCGACTAACGGTCCTTGGATTACGTTTATGAAGCTGAGACTGGACAGAGTGTTAGCAGAAAGTTTTGAAGCTGAGAGTCTAGAAGAAGCGTTAGCCTCCAATCCAACCACTATCGAGTTTGAGAAGCCACAGAAATGGGTAGCACCTTATTCCAAGTATGAATCCGGGTGGTGGGGGCCTTTCTTGCCTCCAAGAACGAAAGAGGAGAGCAAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

227

Amino Acids

25.57

Weight (kDa)

8.32

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 89 - 178 2.5e-35 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AciI CCGC 5 cut(s) 42, 69, 138, 207, 243
AcoI YGGCCR 1 cut(s) 123
AfaI GTAC 2 cut(s) 196, 269
AfiI CCNNNNNNNGG 1 cut(s) 633
AgsI TTSAA 3 cut(s) 157, 215, 530
AhdI GACNNNNNGTC 1 cut(s) 471
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 6 cut(s) 110, 135, 226, 231, 496, 533
AluI AGCT 6 cut(s) 110, 135, 226, 231, 496, 533
Alw26I GTCTC 1 cut(s) 493
AoxI GGCC 3 cut(s) 123, 443, 638
ApeKI GCWGC 2 cut(s) 38, 135
AspS9I GGNCC 2 cut(s) 473, 638
AsuC2I CCSGG 1 cut(s) 628
AvaII GGWCC 1 cut(s) 473
BbvI GCAGC 2 cut(s) 25, 122
BceAI ACGGC 3 cut(s) 29, 194, 434
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 1 cut(s) 323
BcnI CCSGG 1 cut(s) 628
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 4 cut(s) 39, 42, 136, 207
BlpI GCTNAGC 1 cut(s) 227
BlsI GCNGC 4 cut(s) 40, 43, 137, 208
Bme1390I CCNGG 2 cut(s) 323, 628
Bme18I GGWCC 1 cut(s) 473
BmeRI GACNNNNNGTC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 473, 638
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 2 cut(s) 323, 628
Bpu1102I GCTNAGC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 628
BsaJI CCNNGG 2 cut(s) 450, 476
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 317, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 633
Bse1I ACTGG 1 cut(s) 507
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 2 cut(s) 450, 476
BseLI CCNNNNNNNGG 1 cut(s) 633
BseMII CTCAG 4 cut(s) 34, 241, 488, 525
BseNI ACTGG 1 cut(s) 507
BseRI GAGGAG 5 cut(s) 172, 175, 223, 326, 680
BseXI GCAGC 2 cut(s) 25, 122
BseYI CCCAGC 1 cut(s) 33
BshFI GGCC 3 cut(s) 125, 445, 640
BsiSI CCGG 1 cut(s) 627
BslI CCNNNNNNNGG 1 cut(s) 633
BsmAI GTCTC 1 cut(s) 493
BsnI GGCC 3 cut(s) 125, 445, 640
Bsp143I GATC 1 cut(s) 390
Bsp1720I GCTNAGC 1 cut(s) 227
BspACI CCGC 5 cut(s) 42, 69, 138, 207, 243
BspANI GGCC 3 cut(s) 125, 445, 640
BspCNI CTCAG 4 cut(s) 33, 240, 489, 526
BspLI GGNNCC 1 cut(s) 639
BspMI ACCTGC 1 cut(s) 255
BspQI GCTCTTC 1 cut(s) 117
BsrI ACTGG 1 cut(s) 507
BssECI CCNNGG 2 cut(s) 450, 476
BssMI GATC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 31, 447
BstDEI CTNAG 6 cut(s) 20, 227, 399, 429, 497, 534
BstDSI CCRYGG 1 cut(s) 450
BstKTI GATC 1 cut(s) 393
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 1 cut(s) 390
BstMWI GCNNNNNNNGC 2 cut(s) 35, 646
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 33
BstSCI CCNGG 2 cut(s) 321, 626
BstV1I GCAGC 2 cut(s) 25, 122
BstXI CCANNNNNNTGG 1 cut(s) 597
BsuRI GGCC 3 cut(s) 125, 445, 640
BtgI CCRYGG 1 cut(s) 450
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 31, 447
Cfr13I GGNCC 2 cut(s) 473, 638
Csp6I GTAC 2 cut(s) 195, 268
CviAII CATG 1 cut(s) 30
CviQI GTAC 2 cut(s) 195, 268
DdeI CTNAG 6 cut(s) 20, 227, 399, 429, 497, 534
DpnI GATC 1 cut(s) 392
DpnII GATC 1 cut(s) 390
DriI GACNNNNNGTC 1 cut(s) 471
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 117
Eam1105I GACNNNNNGTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 476
Eco147I AGGCCT 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 1 cut(s) 33
FaiI YATR 6 cut(s) 31, 81, 299, 354, 491, 621
FatI CATG 1 cut(s) 29
Fnu4HI GCNGC 4 cut(s) 39, 42, 136, 207
Fsp4HI GCNGC 4 cut(s) 39, 42, 136, 207
FspBI CTAG 1 cut(s) 542
GluI GCNGC 4 cut(s) 39, 42, 136, 207
GsaI CCCAGC 1 cut(s) 37
HaeIII GGCC 3 cut(s) 125, 445, 640
HapII CCGG 1 cut(s) 627
Hin1II CATG 1 cut(s) 33
HinfI GANTC 4 cut(s) 215, 239, 538, 623
HpaII CCGG 1 cut(s) 627
Hpy188I TCNGA 2 cut(s) 220, 238
Hpy188III TCNNGA 2 cut(s) 49, 542
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 163, 435
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 1 cut(s) 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 646
HpyF3I CTNAG 6 cut(s) 20, 227, 399, 429, 497, 534
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 1 cut(s) 33
Kzo9I GATC 1 cut(s) 390
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 3 cut(s) 236, 313, 325
Lsp1109I GCAGC 2 cut(s) 25, 122
MaeI CTAG 1 cut(s) 542
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 10, 140
MalI GATC 1 cut(s) 392
MboI GATC 1 cut(s) 390
MboII GAAGA 4 cut(s) 104, 139, 373, 557
MluCI AATT 1 cut(s) 127
MlyI GAGTC 2 cut(s) 233, 547
MmeI TCCRAC 3 cut(s) 243, 261, 590
MslI CAYNNNNRTG 1 cut(s) 595
MspA1I CMGCKG 2 cut(s) 135, 245
MspI CCGG 1 cut(s) 627
MspR9I CCNGG 2 cut(s) 323, 628
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 35, 646
NciI CCSGG 1 cut(s) 628
NdeII GATC 1 cut(s) 390
NlaIII CATG 1 cut(s) 33
NlaIV GGNNCC 1 cut(s) 639
NmeAIII GCCGAG 2 cut(s) 101, 285
NmuCI GTSAC 2 cut(s) 10, 140
NspI RCATGY 1 cut(s) 33
PaeI GCATGC 1 cut(s) 33
PceI AGGCCT 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 2 cut(s) 215, 623
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 4 cut(s) 40, 43, 137, 208
PleI GAGTC 2 cut(s) 233, 546
PpsI GAGTC 2 cut(s) 233, 546
Psp6I CCWGG 1 cut(s) 321
PspFI CCCAGC 1 cut(s) 33
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 2 cut(s) 473, 638
PvuII CAGCTG 1 cut(s) 135
RsaI GTAC 2 cut(s) 196, 269
RsaNI GTAC 2 cut(s) 195, 268
RseI CAYNNNNRTG 1 cut(s) 595
SapI GCTCTTC 1 cut(s) 117
SatI GCNGC 4 cut(s) 39, 42, 136, 207
Sau3AI GATC 1 cut(s) 390
Sau96I GGNCC 2 cut(s) 473, 638
SchI GAGTC 2 cut(s) 233, 547
ScrFI CCNGG 2 cut(s) 323, 628
SetI ASST 9 cut(s) 112, 137, 228, 233, 269, 488, 498, 535, 610
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 595
SphI GCATGC 1 cut(s) 33
Sse9I AATT 1 cut(s) 127
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 5 cut(s) 42, 69, 138, 207, 243
SspMI CTAG 1 cut(s) 542
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 321, 626
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 1 cut(s) 488
TaqI TCGA 5 cut(s) 303, 346, 393, 464, 576
TasI AATT 1 cut(s) 127
TauI GCSGC 2 cut(s) 44, 209
TfiI GAWTC 2 cut(s) 215, 623
TseFI GTSAC 2 cut(s) 10, 140
TseI GCWGC 2 cut(s) 38, 135
Tsp45I GTSAC 2 cut(s) 10, 140
TspDTI ATGAA 2 cut(s) 506, 636
TspGWI ACGGA 2 cut(s) 178, 182
VpaK11BI GGWCC 1 cut(s) 473
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 1 cut(s) 33
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.