Rh5DG518500

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010918
Physical Location & Seq
Reverse (-)
80574611 .. 80575719
1109 bp
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UTR
Exon/CDS
Intron
Rh5DG518500.1

Sequence Viewer

Length: 435 bp
ATGCAGCAGAAACCGAGCAGGTACTACTTTGTTGTGGCCAATGCCAAGTTCATGCTCGATGAGGAGGAGCATTTTCAGGAGCAACTGTTCGAGCGGCTTCGATTGTTTAAGGAGAGGAACAACGAGCAGGACTTTTGGCTTGTGATCGAGCCCAAGTTCTTGGATAAGTTTCCTGACATTACTAGAAGATTAGGCAGGCCTGCTGTGGCTCTGGTCTCCACTAATGGCCCTTGGATTACATTTATGAAGCTGAGATTGGACCGAGTATTGTCAGAAAGTTTTGAAGCTGAGAGTCTAGAAGAAGCATTAGCCTGCAGTCCAACAAATATTGAGTTCAAGAAGCCAGATAAATGGGTAGCACCATACCCCAAGTATGAATCTGAGTGGTGGGGGCCCTTCTTGCCCCCAGGATCGAAAGAGGAGGCCAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

144

Amino Acids

17.14

Weight (kDa)

5.37

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 7 - 96 1.5e-34 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 9
AccBSI CCGCTC 1 cut(s) 94
AciI CCGC 1 cut(s) 94
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 36
AfaI GTAC 1 cut(s) 23
AgsI TTSAA 2 cut(s) 284, 337
AjnI CCWGG 1 cut(s) 406
AjuI GAANNNNNNNTTGG 4 cut(s) 32, 64, 239, 271
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
AluBI AGCT 2 cut(s) 250, 287
AluI AGCT 2 cut(s) 250, 287
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 1 cut(s) 418
AoxI GGCC 5 cut(s) 36, 197, 226, 392, 423
ApaI GGGCCC 1 cut(s) 396
ApeKI GCWGC 1 cut(s) 4
AspS9I GGNCC 4 cut(s) 227, 259, 392, 393
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 396
BalI TGGCCA 1 cut(s) 38
BanII GRGCYC 2 cut(s) 153, 396
BbvI GCAGC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 408
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 2 cut(s) 183, 296
BfmI CTRYAG 1 cut(s) 313
BfuAI ACCTGC 1 cut(s) 9
BisI GCNGC 2 cut(s) 5, 95
BlsI GCNGC 2 cut(s) 6, 96
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 4 cut(s) 227, 259, 392, 393
BmiI GGNNCC 2 cut(s) 393, 394
BmrFI CCNGG 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 230, 406
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 2 cut(s) 230, 406
BseMII CTCAG 3 cut(s) 242, 279, 372
BseRI GAGGAG 3 cut(s) 77, 80, 434
BseSI GKGCMC 1 cut(s) 396
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 5 cut(s) 38, 199, 228, 394, 425
BsmAI GTCTC 1 cut(s) 220
BsnI GGCC 5 cut(s) 38, 199, 228, 394, 425
Bso31I GGTCTC 1 cut(s) 220
Bsp120I GGGCCC 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 153, 396
Bsp143I GATC 2 cut(s) 144, 410
BspACI CCGC 1 cut(s) 94
BspANI GGCC 5 cut(s) 38, 199, 228, 394, 425
BspCNI CTCAG 3 cut(s) 243, 280, 373
BspLI GGNNCC 2 cut(s) 393, 394
BspMAI CTGCAG 1 cut(s) 317
BspMI ACCTGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 230, 406
BssMI GATC 2 cut(s) 144, 410
BssT1I CCWWGG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 87
BstC8I GCNNGC 3 cut(s) 197, 201, 313
BstDEI CTNAG 3 cut(s) 251, 288, 381
BstKTI GATC 2 cut(s) 147, 413
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 144, 410
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 16
BstXI CCANNNNNNTGG 2 cut(s) 160, 351
BsuRI GGCC 5 cut(s) 38, 199, 228, 394, 425
BveI ACCTGC 1 cut(s) 9
Cac8I GCNNGC 3 cut(s) 197, 201, 313
Cfr13I GGNCC 4 cut(s) 227, 259, 392, 393
Csp6I GTAC 1 cut(s) 22
CviAII CATG 1 cut(s) 52
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 3 cut(s) 251, 288, 381
DpnI GATC 2 cut(s) 146, 412
DpnII GATC 2 cut(s) 144, 410
EaeI YGGCCR 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 230
Eco147I AGGCCT 1 cut(s) 199
Eco24I GRGCYC 2 cut(s) 153, 396
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 259
EcoO109I RGGNCCY 2 cut(s) 392, 393
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 230
EcoT38I GRGCYC 2 cut(s) 153, 396
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 1 cut(s) 55
FaiI YATR 4 cut(s) 53, 245, 364, 375
FatI CATG 1 cut(s) 51
Fnu4HI GCNGC 2 cut(s) 5, 95
FriOI GRGCYC 2 cut(s) 153, 396
Fsp4HI GCNGC 2 cut(s) 5, 95
FspBI CTAG 2 cut(s) 183, 296
GluI GCNGC 2 cut(s) 5, 95
HaeIII GGCC 5 cut(s) 38, 199, 228, 394, 425
Hin1II CATG 1 cut(s) 55
HinfI GANTC 2 cut(s) 292, 377
Hpy188I TCNGA 2 cut(s) 274, 382
Hpy188III TCNNGA 4 cut(s) 77, 173, 296, 337
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 4, 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 3 cut(s) 251, 288, 381
Hsp92II CATG 1 cut(s) 55
Kzo9I GATC 2 cut(s) 144, 410
LmnI GCTCC 2 cut(s) 67, 79
Lsp1109I GCAGC 1 cut(s) 16
MaeI CTAG 2 cut(s) 183, 296
MalI GATC 2 cut(s) 146, 412
MbiI CCGCTC 1 cut(s) 94
MboI GATC 2 cut(s) 144, 410
MboII GAAGA 2 cut(s) 198, 311
MhlI GDGCHC 2 cut(s) 153, 396
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 5 cut(s) 55, 58, 108, 412, 415
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 108
Msp20I TGGCCA 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 2 cut(s) 144, 410
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 2 cut(s) 393, 394
PceI AGGCCT 1 cut(s) 199
PfeI GAWTC 1 cut(s) 377
PkrI GCNGC 2 cut(s) 6, 96
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 393, 394
PspOMI GGGCCC 1 cut(s) 392
PspPI GGNCC 4 cut(s) 227, 259, 392, 393
PstI CTGCAG 1 cut(s) 317
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 5, 95
Sau3AI GATC 2 cut(s) 144, 410
Sau96I GGNCC 4 cut(s) 227, 259, 392, 393
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 2 cut(s) 153, 396
SetI ASST 3 cut(s) 23, 252, 289
SfcI CTRYAG 1 cut(s) 313
SinI GGWCC 1 cut(s) 259
SseBI AGGCCT 1 cut(s) 199
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 328
SspMI CTAG 2 cut(s) 183, 296
StuI AGGCCT 1 cut(s) 199
StyD4I CCNGG 1 cut(s) 406
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 1 cut(s) 87
TaqI TCGA 5 cut(s) 57, 90, 100, 147, 413
TaqII GACCGA 1 cut(s) 276
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 40, 260, 390
VpaK11BI GGWCC 1 cut(s) 259
XbaI TCTAGA 1 cut(s) 295
XspI CTAG 2 cut(s) 183, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.