AT5G61270

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009848
Physical Location & Seq
Reverse (-)
24638491 .. 24640765
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61270.2

Sequence Viewer

Length: 1101 bp
ATGTCGAATTATGGAGTTAAAGAGCTCACATGGGAAAATGGGCAACTAACCGTTCATGGTCTAGGCGACGAAGTAGAACCAACCACCTCGAATAACCCTATTTGGACTCAAAGTCTCAACGGTTGTGAGACTTTGGAGTCTGTGGTTCATCAAGCGGCTCTACAGCAGCCAAGCAAGTTTCAGCTGCAGAGTCCGAATGGTCCAAACCACAATTATGAGAGCAAGGATGGATCTTGTTCAAGAAAACGCGGTTATCCTCAAGAAATGGACCGATGGTTCGCTGTTCAAGAGGAGAGCCATAGAGTTGGCCACAGCGTCACTGCAAGTGCGAGTGGTACCAATATGTCTTGGGCGTCTTTTGAATCCGGTCGGAGCTTGAAGACAGCTAGAACCGGAGACAGAGACTATTTCCGCTCTGGATCGGAAACTCAAGATACTGAAGGAGATGAACAAGAGACAAGAGGAGAAGCAGGTAGATCTAATGGACGACGGGGACGAGCAGCAGCGATTCACAACGAGTCCGAAAGGAGACGGCGTGATAGGATAAACCAGAGGATGAGAACACTTCAGAAGCTGCTTCCTACTGCAAGTAAGGCGGATAAAGTCTCAATCTTGGATGATGTTATCGAACACTTGAAACAGCTACAAGCACAAGTACAGTTCATGAGCCTAAGAGCCAACTTGCCACAACAAATGATGATTCCGCAACTACCTCCACCACAGTCAGTTCTCAGCATCCAACACCAACAACAACAACAACAACAGCAGCAGCAGCAGCAACAACAGCAGCAACAGTTTCAGATGTCGTTGCTTGCAACAATGGCAAGAATGGGAATGGGAGGTGGTGGAAATGGTTATGGAGGTTTAGTTCCTCCTCCTCCTCCTCCACCAATGATGGTCCCTCCTATGGGTAACAGAGACTGCACCAACGGTTCTTCAGCCACATTATCTGATCCATACAGCGCCTTTTTCGCACAGACAATGAATATGGATCTCTACAATAAAATGGCAGCAGCTATCTATAGACAACAGTCTGATCAAACAACAAAGGTAAATATCGGCATGCCTTCAAGTTCTTCGAATCATGAGAAAAGAGATTAG

Protein Analysis

366

Amino Acids

40.83

Weight (kDa)

7.78

Isoelectric Point (pI)

68.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 83 - 128 7.1e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61270 AT5G61270 AT5G61270
fragaria_vesca FvH4_5g14360
malus_domestica MD06G1199400.v1.1 MD14G1208000.v1.1
prunus_persica Prupe.5G200100_v2.0.a1
pyrus_communis pycom06g17620 pycom14g17170
rosa_chinensis RchiOBHm_Chr7g0182341
rosa_laevigata RLG00000005129
rosa_multiflora Rmu_sc0002070.1_g000014
rosa_roxburghii Rroxscaffold_3G00271290
rosa_rugosa Rorug06G0447100
rosa_samantha Rh7AG050200 Rh7AG050400 Rh7BG050200 Rh7CG051500 Rh7CG051700 Rh7DG049400
rosa_wichuraiana Rw7G004110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 461
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccBSI CCGCTC 1 cut(s) 414
AccII CGCG 1 cut(s) 249
AciI CCGC 5 cut(s) 155, 249, 412, 596, 704
AclWI GGATC 4 cut(s) 238, 427, 947, 999
AcoI YGGCCR 1 cut(s) 307
AcuI CTGAAG 3 cut(s) 459, 551, 921
AcyI GRCGYC 1 cut(s) 353
AfaI GTAC 2 cut(s) 337, 657
AfiI CCNNNNNNNGG 2 cut(s) 907, 908
AgsI TTSAA 6 cut(s) 240, 287, 362, 379, 637, 1071
AhdI GACNNNNNGTC 1 cut(s) 111
AloI GAACNNNNNNTCC 4 cut(s) 260, 292, 852, 884
AluBI AGCT 7 cut(s) 25, 184, 375, 386, 574, 643, 1016
AluI AGCT 7 cut(s) 25, 184, 375, 386, 574, 643, 1016
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 8 cut(s) 119, 122, 390, 396, 449, 523, 610, 912
AlwI GGATC 4 cut(s) 238, 427, 947, 999
AlwNI CAGNNNCTG 2 cut(s) 574, 921
AoxI GGCC 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 965
AspS9I GGNCC 3 cut(s) 200, 268, 898
AsuII TTCGAA 1 cut(s) 1079
AvaII GGWCC 3 cut(s) 200, 268, 898
BalI TGGCCA 1 cut(s) 309
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 27
BccI CCATC 3 cut(s) 221, 267, 889
BceAI ACGGC 1 cut(s) 548
BclI TGATCA 1 cut(s) 1036
BcoDI GTCTC 8 cut(s) 119, 122, 390, 396, 449, 523, 610, 912
BfaI CTAG 2 cut(s) 62, 387
BfmI CTRYAG 3 cut(s) 161, 185, 1021
BfoI RGCGCY 1 cut(s) 966
BfuAI ACCTGC 1 cut(s) 461
BglII AGATCT 1 cut(s) 476
Bme18I GGWCC 3 cut(s) 200, 268, 898
BmeRI GACNNNNNGTC 1 cut(s) 111
BmgT120I GGNCC 3 cut(s) 200, 268, 898
BmiI GGNNCC 2 cut(s) 337, 900
BmsI GCATC 1 cut(s) 744
BoxI GACNNNNGTC 1 cut(s) 1030
BpiI GAAGAC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 1079
BpuEI CTTGAG 2 cut(s) 243, 414
BsaHI GRCGYC 1 cut(s) 353
BsaWI WCCGGW 2 cut(s) 365, 392
BsaXI ACNNNNNCTCC 4 cut(s) 520, 550, 852, 882
Bsc4I CCNNNNNNNGG 2 cut(s) 907, 908
BseGI GGATG 4 cut(s) 232, 561, 622, 735
BseLI CCNNNNNNNGG 2 cut(s) 907, 908
BseMII CTCAG 1 cut(s) 745
BseRI GAGGAG 6 cut(s) 305, 477, 864, 867, 870, 873
BsgI GTGCAG 1 cut(s) 907
Bsh1236I CGCG 1 cut(s) 249
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 2 cut(s) 366, 393
BslFI GGGAC 2 cut(s) 507, 884
BslI CCNNNNNNNGG 2 cut(s) 907, 908
BsmAI GTCTC 8 cut(s) 119, 122, 390, 396, 449, 523, 610, 912
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 2 cut(s) 507, 884
BsnI GGCC 1 cut(s) 309
Bsp119I TTCGAA 1 cut(s) 1079
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
BspACI CCGC 5 cut(s) 155, 249, 412, 596, 704
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 1 cut(s) 744
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 2 cut(s) 663, 1084
BspLI GGNNCC 2 cut(s) 337, 900
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 4 cut(s) 238, 427, 947, 999
BspT104I TTCGAA 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 414
BssMI GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
BssNI GRCGYC 1 cut(s) 353
Bst4CI ACNGT 7 cut(s) 52, 122, 660, 723, 795, 932, 1032
BstACI GRCGYC 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 1079
BstC8I GCNNGC 2 cut(s) 813, 1064
BstDEI CTNAG 2 cut(s) 671, 731
BstF5I GGATG 4 cut(s) 232, 561, 622, 735
BstFNI CGCG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 966
BstHHI GCGC 1 cut(s) 965
BstKTI GATC 6 cut(s) 233, 422, 479, 955, 994, 1039
BstMAI GTCTC 8 cut(s) 119, 122, 390, 396, 449, 523, 610, 912
BstMBI GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 6 cut(s) 593, 772, 775, 784, 821, 971
BstNSI RCATGY 1 cut(s) 1066
BstPAI GACNNNNGTC 1 cut(s) 1030
BstSFI CTRYAG 3 cut(s) 161, 185, 1021
BstUI CGCG 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 3 cut(s) 230, 476, 991
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 3 cut(s) 230, 476, 991
BsuRI GGCC 1 cut(s) 309
BtsCI GGATG 4 cut(s) 232, 561, 622, 735
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 318
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 2 cut(s) 813, 1064
CaiI CAGNNNCTG 2 cut(s) 574, 921
CciI TCATGA 2 cut(s) 663, 1084
CfoI GCGC 1 cut(s) 965
Cfr13I GGNCC 3 cut(s) 200, 268, 898
CseI GACGC 2 cut(s) 304, 342
Csp6I GTAC 2 cut(s) 336, 656
CviAII CATG 5 cut(s) 30, 56, 664, 1063, 1085
CviQI GTAC 2 cut(s) 336, 656
DdeI CTNAG 2 cut(s) 671, 731
DpnI GATC 6 cut(s) 232, 421, 478, 954, 993, 1038
DpnII GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
DrdI GACNNNNNNGTC 1 cut(s) 136
DriI GACNNNNNGTC 1 cut(s) 111
DseDI GACNNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 307
Eam1105I GACNNNNNGTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 611
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 3 cut(s) 200, 268, 898
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 3 cut(s) 459, 551, 921
EcoICRI GAGCTC 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 5 cut(s) 33, 59, 667, 1066, 1088
FaqI GGGAC 2 cut(s) 507, 884
FatI CATG 5 cut(s) 29, 55, 663, 1062, 1084
FbaI TGATCA 1 cut(s) 1036
FokI GGATG 4 cut(s) 239, 568, 629, 722
FriOI GRGCYC 1 cut(s) 27
FspBI CTAG 2 cut(s) 62, 387
GlaI GCGC 1 cut(s) 964
HaeII RGCGCY 1 cut(s) 966
HaeIII GGCC 1 cut(s) 309
HapII CCGG 2 cut(s) 366, 393
HgaI GACGC 2 cut(s) 304, 342
HhaI GCGC 1 cut(s) 965
Hin1I GRCGYC 1 cut(s) 353
Hin1II CATG 5 cut(s) 33, 59, 667, 1066, 1088
Hin6I GCGC 1 cut(s) 963
HinP1I GCGC 1 cut(s) 963
HinfI GANTC 8 cut(s) 106, 137, 190, 362, 508, 518, 700, 1081
HpaII CCGG 2 cut(s) 366, 393
Hpy188I TCNGA 8 cut(s) 195, 372, 424, 523, 570, 801, 952, 1036
Hpy188III TCNNGA 7 cut(s) 240, 260, 287, 417, 431, 664, 1085
Hpy99I CGWCG 2 cut(s) 71, 492
HpyAV CCTTC 2 cut(s) 434, 1077
HpyCH4III ACNGT 7 cut(s) 52, 122, 660, 723, 795, 932, 1032
HpyCH4V TGCA 5 cut(s) 187, 323, 587, 815, 924
HpyF10VI GCNNNNNNNGC 6 cut(s) 593, 772, 775, 784, 821, 971
HpyF3I CTNAG 2 cut(s) 671, 731
Hsp92I GRCGYC 1 cut(s) 353
Hsp92II CATG 5 cut(s) 33, 59, 667, 1066, 1088
HspAI GCGC 1 cut(s) 963
KpnI GGTACC 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 1036
Kzo9I GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
LmnI GCTCC 1 cut(s) 372
LpnPI CCDG 5 cut(s) 379, 402, 406, 456, 563
LweI GCATC 1 cut(s) 744
MaeI CTAG 2 cut(s) 62, 387
MaeIII GTNAC 2 cut(s) 316, 911
MalI GATC 6 cut(s) 232, 421, 478, 954, 993, 1038
MbiI CCGCTC 1 cut(s) 414
MboI GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
MboII GAAGA 3 cut(s) 391, 927, 1068
MflI RGATCY 3 cut(s) 230, 476, 991
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 2 cut(s) 7, 211
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 4 cut(s) 100, 146, 199, 527
MmeI TCCRAC 2 cut(s) 350, 763
Mox20I TGGCCA 1 cut(s) 309
MscI TGGCCA 1 cut(s) 309
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 2 cut(s) 366, 393
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 6 cut(s) 593, 772, 775, 784, 821, 971
NdeII GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
NlaIII CATG 5 cut(s) 33, 59, 667, 1066, 1088
NlaIV GGNNCC 2 cut(s) 337, 900
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 1 cut(s) 1066
NspV TTCGAA 1 cut(s) 1079
PaeI GCATGC 1 cut(s) 1066
PagI TCATGA 2 cut(s) 663, 1084
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PfeI GAWTC 4 cut(s) 362, 508, 700, 1081
PleI GAGTC 4 cut(s) 100, 145, 198, 526
PpsI GAGTC 4 cut(s) 100, 145, 198, 526
PshAI GACNNNNGTC 1 cut(s) 1030
Psp124BI GAGCTC 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 337, 900
PspPI GGNCC 3 cut(s) 200, 268, 898
PstI CTGCAG 1 cut(s) 189
PstNI CAGNNNCTG 2 cut(s) 574, 921
PsuI RGATCY 3 cut(s) 230, 476, 991
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 2 cut(s) 337, 657
RsaNI GTAC 2 cut(s) 336, 656
RseI CAYNNNNRTG 1 cut(s) 213
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 18
Sau3AI GATC 6 cut(s) 230, 419, 476, 952, 991, 1036
Sau96I GGNCC 3 cut(s) 200, 268, 898
SchI GAGTC 4 cut(s) 100, 146, 199, 527
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 1 cut(s) 744
SfcI CTRYAG 3 cut(s) 161, 185, 1021
SfuI TTCGAA 1 cut(s) 1079
SinI GGWCC 3 cut(s) 200, 268, 898
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 2 cut(s) 258, 429
SmoI CTYRAG 2 cut(s) 258, 429
SphI GCATGC 1 cut(s) 1066
Sse9I AATT 2 cut(s) 7, 211
SsiI CCGC 5 cut(s) 155, 249, 412, 596, 704
SspMI CTAG 2 cut(s) 62, 387
SstI GAGCTC 1 cut(s) 27
TaaI ACNGT 7 cut(s) 52, 122, 660, 723, 795, 932, 1032
TaqI TCGA 4 cut(s) 5, 89, 627, 1079
TaqII GACCGA 1 cut(s) 285
TasI AATT 2 cut(s) 7, 211
TatI WGTACW 1 cut(s) 655
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 4 cut(s) 362, 508, 700, 1081
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 5 cut(s) 44, 137, 462, 652, 998
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 3 cut(s) 200, 268, 898
XceI RCATGY 1 cut(s) 1066
XspI CTAG 2 cut(s) 62, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.