MD14G1208000.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009848
Physical Location & Seq
Reverse (-)
29482039 .. 29483412
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1208000.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGCATGGGCTTGGTGCGCTTCTTACAGCTCAATCAAATCCCACATGGAGCAGGAGCAGGGCTTGTGACACACTGGAATCAATTGTCGATCAAGCTACATGCCACAAACGTGACCCAAATATCATCCGCCATGACCAGACCCCGGCAAACACAAGCTCGGTGGTTGCATCCTCCGGCGAAACATGGACCGACAGCTCTGTCCAGGTGCCGCCGGCGCAGGGGTGGATAAGAAAACGCAGTCGATCATATTCCGGCTTCTTCGAGAATAACTTTAGTACTAATAGCATTCATGAAGAACATGCAGATCCCAGCAGTTGTGCTAGTCCTGGTGCTAGTGTTAGATTGTGCAGGGACAATCATAAAACTACCTCGACGTTAGTTTCTTTTGAGTCTCCTCCCAGTTTGAAGACCAAAAGCACCGACGAGGATTCGGCCAGTCACGGAGGGCTGGAAAACCGAGATGATGGCCGGGAAACCACCAAAGGTGGCGGATCAAGCCGTTCACACTCAACAAGACCAAGGCGAGCTGCTGCTGTTCATAATCAGTCAGAGCGGAAACGAAGAGACTTGATTAACCAGAAGATAAAAGCTCTGCAAAGGTTGGTGCCAAATGCAAGTAAGACTGATAAAGCTTCAATGCTTGATGAGGTGATCAAATACTTGCAACAACTTCAAGCTCAAGTCCAAATGATGCATAATCTGAGAAATTTCATGGCTAATGGCCATATGAATATGAATATGATGGTGCCTTTAGAAATGCAGCAGCTGCAGCTGCAGCATCTTCATCAAATGTCACTCCTGGCTCATATGGGAATCGGAATGGGATCGCGCATTCATCCTACGCCGGTTGTGGCTGCCGCTCTTCCTTCTTTCATGCCGCCATTTATGATGCCGCTGTTAATCCCACCACACCCTCCTCCTCAAGCAAAGGCAGAACCAACCAGCACGAATAATGGTTCAGACCCTCTACCTGATCCATATTGTGCCCTCCTAGCACAAGTACGTGTTTCCACATACATATGTGAGGAAATGATAGAAAGAAACCTATGTTTGGAAGTACTTCTAGAATAG

Protein Analysis

357

Amino Acids

39.36

Weight (kDa)

8.18

Isoelectric Point (pI)

62.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 179 - 225 5.2e-13 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 183 - 230 3e-06 Transcription factor SAC51 bHLH domain
bHLH_LHW PF23176 187 - 234 2.8e-06 Transcription factor LHW bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61270 AT5G61270 AT5G61270
fragaria_vesca FvH4_5g14360
malus_domestica MD06G1199400.v1.1 MD14G1208000.v1.1
prunus_persica Prupe.5G200100_v2.0.a1
pyrus_communis pycom06g17620 pycom14g17170
rosa_chinensis RchiOBHm_Chr7g0182341
rosa_laevigata RLG00000005129
rosa_multiflora Rmu_sc0002070.1_g000014
rosa_roxburghii Rroxscaffold_3G00271290
rosa_rugosa Rorug06G0447100
rosa_samantha Rh7AG050200 Rh7AG050400 Rh7BG050200 Rh7CG051500 Rh7CG051700 Rh7DG049400
rosa_wichuraiana Rw7G004110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AccB1I GGYRCC 3 cut(s) 205, 604, 745
AccBSI CCGCTC 2 cut(s) 553, 860
AccII CGCG 1 cut(s) 829
AciI CCGC 7 cut(s) 127, 209, 489, 553, 858, 878, 893
AclWI GGATC 4 cut(s) 299, 499, 832, 968
AcoI YGGCCR 3 cut(s) 432, 466, 721
AcsI RAATTY 1 cut(s) 706
AfaI GTAC 3 cut(s) 277, 1002, 1059
AfiI CCNNNNNNNGG 3 cut(s) 142, 218, 1051
AflIII ACRYGT 1 cut(s) 1003
AgsI TTSAA 3 cut(s) 406, 636, 674
AjnI CCWGG 3 cut(s) 201, 325, 798
AleI CACNNNNGTG 1 cut(s) 108
Alw26I GTCTC 2 cut(s) 396, 558
AlwI GGATC 4 cut(s) 299, 499, 832, 968
AlwNI CAGNNNCTG 1 cut(s) 766
AoxI GGCC 3 cut(s) 432, 466, 721
ApeKI GCWGC 9 cut(s) 527, 530, 760, 763, 766, 769, 772, 775, 854
ApoI RAATTY 1 cut(s) 706
Asp700I GAANNNNTTC 1 cut(s) 1059
AspLEI GCGC 3 cut(s) 19, 217, 831
AspS9I GGNCC 1 cut(s) 186
AsuC2I CCSGG 2 cut(s) 143, 470
AsuHPI GGTGA 1 cut(s) 661
AvaII GGWCC 1 cut(s) 186
BaeGI GKGCMC 1 cut(s) 988
BalI TGGCCA 1 cut(s) 723
BanI GGYRCC 3 cut(s) 205, 604, 745
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 9 cut(s) 514, 517, 753, 759, 772, 775, 781, 787, 841
BccI CCATC 2 cut(s) 458, 736
BceAI ACGGC 1 cut(s) 483
BciT130I CCWGG 3 cut(s) 203, 327, 800
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 2 cut(s) 143, 470
BcoDI GTCTC 2 cut(s) 396, 558
BfaI CTAG 4 cut(s) 321, 333, 992, 1064
BfmI CTRYAG 2 cut(s) 767, 773
BmcAI AGTACT 2 cut(s) 277, 1059
Bme1390I CCNGG 5 cut(s) 143, 203, 327, 470, 800
Bme18I GGWCC 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 3 cut(s) 207, 606, 747
BmrFI CCNGG 5 cut(s) 143, 203, 327, 470, 800
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 4 cut(s) 176, 681, 787, 879
BmuI ACTGGG 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 663, 906
BpuMI CCSGG 2 cut(s) 143, 470
BsaAI YACGTR 1 cut(s) 1004
BsaJI CCNNGG 2 cut(s) 141, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 218, 1051
Bse118I RCCGGY 2 cut(s) 211, 844
Bse1I ACTGG 3 cut(s) 78, 399, 435
BseBI CCWGG 3 cut(s) 203, 327, 800
BseDI CCNNGG 2 cut(s) 141, 518
BseGI GGATG 3 cut(s) 123, 167, 835
BseLI CCNNNNNNNGG 3 cut(s) 142, 218, 1051
BseMII CTCAG 1 cut(s) 692
BseNI ACTGG 3 cut(s) 78, 399, 435
BseRI GAGGAG 3 cut(s) 384, 906, 909
BseSI GKGCMC 1 cut(s) 988
BseXI GCAGC 9 cut(s) 514, 517, 753, 759, 772, 775, 781, 787, 841
BseYI CCCAGC 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 367
Bsh1236I CGCG 1 cut(s) 829
BshFI GGCC 3 cut(s) 434, 468, 723
BshNI GGYRCC 3 cut(s) 205, 604, 745
BsiSI CCGG 6 cut(s) 143, 174, 212, 252, 469, 845
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 3 cut(s) 142, 218, 1051
BsmAI GTCTC 2 cut(s) 396, 558
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 2 cut(s) 285, 831
BsnI GGCC 3 cut(s) 434, 468, 723
Bsp1286I GDGCHC 1 cut(s) 988
Bsp143I GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
BspACI CCGC 7 cut(s) 127, 209, 489, 553, 858, 878, 893
BspANI GGCC 3 cut(s) 434, 468, 723
BspCNI CTCAG 1 cut(s) 693
BspFNI CGCG 1 cut(s) 829
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 207, 606, 747
BspMAI CTGCAG 2 cut(s) 771, 777
BspPI GGATC 4 cut(s) 299, 499, 832, 968
BspQI GCTCTTC 1 cut(s) 867
BspT107I GGYRCC 3 cut(s) 205, 604, 745
BsrBI CCGCTC 2 cut(s) 553, 860
BsrFI RCCGGY 2 cut(s) 211, 844
BsrI ACTGG 3 cut(s) 78, 399, 435
BssAI RCCGGY 2 cut(s) 211, 844
BssECI CCNNGG 2 cut(s) 141, 518
BssMI GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
BssT1I CCWWGG 1 cut(s) 518
Bst2UI CCWGG 3 cut(s) 203, 327, 800
Bst6I CTCTTC 2 cut(s) 556, 867
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 1004
BstC8I GCNNGC 2 cut(s) 213, 525
BstDEI CTNAG 1 cut(s) 701
BstF5I GGATG 3 cut(s) 123, 167, 835
BstFNI CGCG 1 cut(s) 829
BstHHI GCGC 3 cut(s) 19, 217, 831
BstKTI GATC 7 cut(s) 91, 245, 307, 494, 654, 827, 976
BstMAI GTCTC 2 cut(s) 396, 558
BstMBI GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
BstMWI GCNNNNNNNGC 8 cut(s) 16, 214, 495, 766, 769, 772, 775, 992
BstNI CCWGG 3 cut(s) 203, 327, 800
BstNSI RCATGY 2 cut(s) 102, 302
BstSCI CCNGG 5 cut(s) 141, 201, 325, 468, 798
BstSFI CTRYAG 2 cut(s) 767, 773
BstSLI GKGCMC 1 cut(s) 988
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 9 cut(s) 514, 517, 753, 759, 772, 775, 781, 787, 841
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 3 cut(s) 434, 468, 723
BtsCI GGATG 3 cut(s) 123, 167, 835
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 2 cut(s) 213, 525
CaiI CAGNNNCTG 1 cut(s) 766
CciI TCATGA 1 cut(s) 289
CfoI GCGC 3 cut(s) 19, 217, 831
Cfr10I RCCGGY 2 cut(s) 211, 844
Cfr13I GGNCC 1 cut(s) 186
Csp6I GTAC 3 cut(s) 276, 1001, 1058
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviAII CATG 9 cut(s) 5, 45, 99, 131, 183, 290, 299, 712, 874
CviQI GTAC 3 cut(s) 276, 1001, 1058
DdeI CTNAG 1 cut(s) 701
DpnI GATC 7 cut(s) 90, 244, 306, 493, 653, 826, 975
DpnII GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 3 cut(s) 432, 466, 721
Eam1104I CTCTTC 2 cut(s) 556, 867
EarI CTCTTC 2 cut(s) 556, 867
EciI GGCGGA 2 cut(s) 116, 504
Eco130I CCWWGG 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 186
EcoRII CCWGG 3 cut(s) 201, 325, 798
EcoT14I CCWWGG 1 cut(s) 518
EcoT22I ATGCAT 2 cut(s) 6, 696
ErhI CCWWGG 1 cut(s) 518
FaeI CATG 9 cut(s) 8, 48, 102, 134, 186, 293, 302, 715, 877
FaqI GGGAC 1 cut(s) 365
FatI CATG 9 cut(s) 4, 44, 98, 130, 182, 289, 298, 711, 873
FauNDI CATATG 3 cut(s) 726, 807, 1019
FbaI TGATCA 1 cut(s) 651
FokI GGATG 3 cut(s) 110, 154, 822
FspBI CTAG 4 cut(s) 321, 333, 992, 1064
GlaI GCGC 3 cut(s) 18, 216, 830
GsaI CCCAGC 1 cut(s) 312
HaeIII GGCC 3 cut(s) 434, 468, 723
HapII CCGG 6 cut(s) 143, 174, 212, 252, 469, 845
HhaI GCGC 3 cut(s) 19, 217, 831
Hin1II CATG 9 cut(s) 8, 48, 102, 134, 186, 293, 302, 715, 877
Hin6I GCGC 3 cut(s) 17, 215, 829
HinP1I GCGC 3 cut(s) 17, 215, 829
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 4 cut(s) 77, 389, 428, 813
HpaII CCGG 6 cut(s) 143, 174, 212, 252, 469, 845
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 1 cut(s) 503
Hpy188I TCNGA 4 cut(s) 550, 702, 818, 961
Hpy188III TCNNGA 3 cut(s) 262, 290, 1064
Hpy8I GTNNAC 1 cut(s) 503
Hpy99I CGWCG 2 cut(s) 376, 425
HpyAV CCTTC 1 cut(s) 876
HpyCH4IV ACGT 3 cut(s) 109, 374, 1003
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 214, 495, 766, 769, 772, 775, 992
HpyF3I CTNAG 1 cut(s) 701
HpySE526I ACGT 3 cut(s) 109, 374, 1003
Hsp92II CATG 9 cut(s) 8, 48, 102, 134, 186, 293, 302, 715, 877
HspAI GCGC 3 cut(s) 17, 215, 829
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 2 cut(s) 48, 54
Lsp1109I GCAGC 9 cut(s) 514, 517, 753, 759, 772, 775, 781, 787, 841
LweI GCATC 4 cut(s) 176, 681, 787, 879
MaeI CTAG 4 cut(s) 321, 333, 992, 1064
MaeII ACGT 3 cut(s) 109, 374, 1003
MaeIII GTNAC 4 cut(s) 65, 110, 437, 792
MalI GATC 7 cut(s) 90, 244, 306, 493, 653, 826, 975
MbiI CCGCTC 2 cut(s) 553, 860
MboI GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
MboII GAAGA 7 cut(s) 250, 305, 418, 573, 592, 773, 854
MfeI CAATTG 1 cut(s) 81
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 988
MlsI TGGCCA 1 cut(s) 723
MluCI AATT 2 cut(s) 81, 706
MluNI TGGCCA 1 cut(s) 723
MlyI GAGTC 1 cut(s) 398
Mox20I TGGCCA 1 cut(s) 723
Mph1103I ATGCAT 2 cut(s) 6, 696
MreI CGCCGGCG 1 cut(s) 211
MroNI GCCGGC 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 1059
MscI TGGCCA 1 cut(s) 723
MseI TTAA 2 cut(s) 573, 899
MslI CAYNNNNRTG 2 cut(s) 108, 1018
Msp20I TGGCCA 1 cut(s) 723
MspA1I CMGCKG 3 cut(s) 766, 772, 895
MspI CCGG 6 cut(s) 143, 174, 212, 252, 469, 845
MspR9I CCNGG 5 cut(s) 143, 203, 327, 470, 800
MunI CAATTG 1 cut(s) 81
Mva1269I GAATGC 2 cut(s) 285, 831
MvaI CCWGG 3 cut(s) 203, 327, 800
MvnI CGCG 1 cut(s) 829
MwoI GCNNNNNNNGC 8 cut(s) 16, 214, 495, 766, 769, 772, 775, 992
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 2 cut(s) 143, 470
NdeI CATATG 3 cut(s) 726, 807, 1019
NdeII GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 9 cut(s) 8, 48, 102, 134, 186, 293, 302, 715, 877
NlaIV GGNNCC 3 cut(s) 207, 606, 747
NmuCI GTSAC 4 cut(s) 65, 110, 437, 792
NsiI ATGCAT 2 cut(s) 6, 696
NspI RCATGY 2 cut(s) 102, 302
OliI CACNNNNGTG 1 cut(s) 108
PagI TCATGA 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 867
PctI GAATGC 2 cut(s) 285, 831
PdiI GCCGGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 1059
PfeI GAWTC 3 cut(s) 77, 428, 813
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Ppu21I YACGTR 1 cut(s) 1004
Psp6I CCWGG 3 cut(s) 201, 325, 798
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 3 cut(s) 201, 325, 798
PspN4I GGNNCC 3 cut(s) 207, 606, 747
PspPI GGNCC 1 cut(s) 186
PstI CTGCAG 2 cut(s) 771, 777
PstNI CAGNNNCTG 1 cut(s) 766
PsuI RGATCY 1 cut(s) 304
PvuII CAGCTG 2 cut(s) 766, 772
RsaI GTAC 3 cut(s) 277, 1002, 1059
RsaNI GTAC 3 cut(s) 276, 1001, 1058
RseI CAYNNNNRTG 2 cut(s) 108, 1018
SapI GCTCTTC 1 cut(s) 867
SaqAI TTAA 2 cut(s) 573, 899
Sau3AI GATC 7 cut(s) 88, 242, 304, 491, 651, 824, 973
Sau96I GGNCC 1 cut(s) 186
ScaI AGTACT 2 cut(s) 277, 1059
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 5 cut(s) 143, 203, 327, 470, 800
SduI GDGCHC 1 cut(s) 988
SfaNI GCATC 4 cut(s) 176, 681, 787, 879
SfcI CTRYAG 2 cut(s) 767, 773
SgrAI CRCCGGYG 1 cut(s) 211
SinI GGWCC 1 cut(s) 186
SmiMI CAYNNNNRTG 2 cut(s) 108, 1018
SmlI CTYRAG 2 cut(s) 678, 921
SmoI CTYRAG 2 cut(s) 678, 921
Sse9I AATT 2 cut(s) 81, 706
SsiI CCGC 7 cut(s) 127, 209, 489, 553, 858, 878, 893
SspMI CTAG 4 cut(s) 321, 333, 992, 1064
StyD4I CCNGG 5 cut(s) 141, 201, 325, 468, 798
StyI CCWWGG 1 cut(s) 518
TaiI ACGT 3 cut(s) 112, 377, 1006
TaqI TCGA 4 cut(s) 87, 241, 261, 371
TaqII GACCGA 1 cut(s) 203
TasI AATT 2 cut(s) 81, 706
TatI WGTACW 2 cut(s) 275, 1057
TauI GCSGC 4 cut(s) 211, 860, 880, 895
TfiI GAWTC 3 cut(s) 77, 428, 813
Tru1I TTAA 2 cut(s) 573, 899
Tru9I TTAA 2 cut(s) 573, 899
TscAI CASTG 1 cut(s) 78
TseFI GTSAC 4 cut(s) 65, 110, 437, 792
TseI GCWGC 9 cut(s) 527, 530, 760, 763, 766, 769, 772, 775, 854
Tsp45I GTSAC 4 cut(s) 65, 110, 437, 792
TspDTI ATGAA 9 cut(s) 278, 306, 527, 700, 743, 749, 773, 824, 862
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 78
VpaK11BI GGWCC 1 cut(s) 186
XapI RAATTY 1 cut(s) 706
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 2 cut(s) 102, 302
XmnI GAANNNNTTC 1 cut(s) 1059
XspI CTAG 4 cut(s) 321, 333, 992, 1064
ZrmI AGTACT 2 cut(s) 277, 1059
Zsp2I ATGCAT 2 cut(s) 6, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.