AT5G64420

DNA polymerase phi

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010240
Physical Location & Seq
Forward (+)
25756272 .. 25761516
5245 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64420.1

Sequence Viewer

Length: 3921 bp
ATGGGTAGTAAGAAGAGAAGCAACGACGACTCGACGGAGTTGGTGGAGAACGACAACCTCCCCGATTCTTCAATTGTGAAGAAGAAGAAATCTAAAACTGAGAAGATGAATACCGTTGCTAATTATGATTCTGATACGGCGGCGGCGGCGGCGGAAGTTCCTGGTGTTGCGAGCTCCGGTAAGGATATGGAGAAGAAGAAGATGAGAAAAGCTTCTGATAAACAGAGGAGACTAGAAGCTGCTCTGAAGAAAAACAATGGTGTAGGAGCTTCGCGGCCTAAGCCTATACCTGTGGCTGTAAATTCAAACTCCGATGAAGCCGACGACGAGTCTCTTCCCTCCGCAGCCGCTTCTTCTTCCTCGTTGCCTCTGAACTATTTTACAGATTTGGCTTCTTCTGATGCTTCCGTTAGAGAAGCAGCTGCTGAGTCGTTGGTATTGAGGTTGCAAGAGATTCAGAAGCAATACGAAATGTTACCTGATAAAGAATCTGTTGATGGTGGTTTGATGCTTGAAGCTGAGAAAAACGATGGTTTGGATAACTGTGCTCCTCATCTGAGATATGCTCTACGCAGGCTTATTCGTGGAGTCTCTTCTTCAAGAGAGTGCGCAAGACAAGGATTTGCTTTGGGGTTGACATTACCTGTTAGCGTTATTTCTAGCATTAATGTGGAATCTTTGCTGAATCTCATTGCTGATTCTTTGTCTGTATCTTCATCCATGACGGGACAAGATATAAAGGAATGCTTATTGGGTCGATTGTTTGCTTATGGTGCGCTTGCACGTTCTGGAAGACTGATTGAAGATTGGCAATCTGATAAAGACTCTCCGATAATCAAAGAATTTACCAATGCTCTTATTGGTCTTGCTGCAAAAAAGCGTTACTTGCAGGAACCAGCTGTGCATATTCTTCTAGACTTTGTTGACAAGTTGCCTACTGAACCTGTTGTTACTCATGTTATGGAAGCTCCAGAACTTTATAAATGGTTTGAACAAGCCACCGAGGTGGGAAACCCAGATGCTTTGCTCTTAGCGCTGAAACTCCATGAAAAGGTTTCAGTCGATCATCCTATATTTAGCAAGCTACTGCCAGTTCCTTTCAGTTCCGGGAAGTTTTTTTCTGCTGATCATCTCACTGCCATTGGTAATTGCTTGAAGGAATCTACTTTCTGCCAGCCTCGTGTTCATAGCTTATGGCCTGTTATAGTCGATATGCTGTTACCGGAAGCAGTTGTTCAAAGCGAAGATGTCGTATCTGTTTCAAGTTCCTCGAAAAAGCAAAAACGTAACCGGAAGTCTAATCCAGTTGAAGAAGAAGCCACAAATAACATCAGAAATTTCTGCGAAGTTTTTATGGAAGGAGACCTACTTTCTTCAACTCATGTTCGCAAGCATTTGGCATTTGATATCCTGCTTCTTCTTCTTCCAAAGCTTCCTGCAAGTTTTATCCAACATGTATTGTCGCTCAAATTTGTTCAGTGCCTCATGGATATACTCTCCACAAAGGACTCTTGGTTGCATAAAGTTGCCACCCATTTTCTTGTCGAGTTAATGGATTGGGTGAAAGATGACGATACCAAGAGGGTGGCCGTTACAATGGCTCTCCAGAAACACAGCGAAGGAAAATTTGATAATATTACGCGGACAAAAACTGTTAAAGTTCTAGCTGCAGAGCTTGAAACTGAAGATGGTTGCACACTTTATCTCCAGAACTTGATGAATTTGTTTGTGGATGAACAGCATGTTCCTGAAGAATCTTCAAATATGAAATGGTCCCTAGAACCTTGCTCTTTGAATTCAGATCAGAGTCAAACAACGGATGATAATTCTGACAATGGTTCAAATGAGGAGAAGGATTCAGTTGGAACAACCGGAAACTCGGATGTTCTGAAAAGCTGGGTCATTGAATCTCTCCCTGGCATCTTAAAACATGCTAAATTGTCCCCTGAGGCGAAACTGCGACTTCAGAAGCAGATCCTGAAATTTCTCGCTGTACAAGGTCTCTTTCTGGCTTCTCTGGGCACTGAAGTTACATCATTTGAGTTGCAGGAGAAATTTAAGTGGCCAAAGACAGCTACTCCTGCTGCTCTTTGCCAAATGTGCATCGAACAGCTTCAGCTGTTGCTGTCAAATTCTCAGAAGATTGAGAAACCACTATCTAAAGGAAATGCCCTGGAACAGCCCGATGATCCTGTTTCATACTTTATGAAATTCCTTAGCACGTTGCAGAATATTCCCTCAGTTTCCCTCTTCCGTTCCTTGAATGAAGCAGATGAAAAGGCTTTCAAAGAATTACAAGAAACAGAAAGTAAACTTTTAAAGGAGGAAAGAAATTGTGGACTATCTACTGATCCAAATAAATTTCATGCATTGAGGCACTTAGTTGTTCAGCTGTTGCTTCAAATACTCCTCCATCCGGGAGAATTCTCAGAAGCTGCTACTGAACTTTCAGTATGCTGTGACAAAGCTTTCAGTTCTCTTGATCTTCTGAAAAGTGATGGGCAAGGTGAGGCAGACGATGAAGAAGAACCAGCAGTCATGGATGTCCTCGTGGACACATTACTTTCTCTCTTGCCGCATTCTTCAGCTCCAATGCGGTCTTCTATTGAGCAGGTTTTTAAGTACTTTTGTCAAGATGTCACCAACGATGGACTGCTGAGAATGTTGCGGGTGATCAAGAAAGATCTAAAACCGTCTAGACATCAGGAGGACCAAGACTCTGACGATCTTGATGATGATGAAGAAGATTGTCTTGCTATTGAAGATGCAGAAGAAGAAAACGAGGAGATGGGTGAAACTGGTGAGAGCGATGAGCAGACTGATGATTCCGAAGCAGTAACCGGCGTTGTCCCTATGGCAGTTGACAGAGAAGTTCCTGAAAATTCCGATGATTCTGATGATGATGATGGAATGGATGATGATGCAATGTTCCGCATGGACACTTACCTTGCCCAGATATTCAAGGAGAAGAGGAATCAAGCTGGCGGCGAAACTGCTCAGTCCCAGCTTGTTTTGTTTAAACTCCGTGTCCTCTCCCTTCTTGAAATCTACCTCCATGAAAATTCAGATAAGCCACAAGTTATGACAGTGTACTTAAACTTAGTTCAGGCAATGCTGAACCCAAGCACTGCAGAAAGTAGTCTGCCGCTTTTACAACGTATATGGGGCATCATTCAGAAGAAGATCTTCAAGGCTAAAGAGTTTCCCAAGGATGAATCAATGGAATTTTCTGCACTTGCTTCTTTGTTGGAGAAGAATCTTAAACTGGCTGCGAAACCATTCAAGAGTAAGAAGTCAGGAGTTGACCCTTCCAAGAAGAAGCAGTCAGCTGCGTGGAACCGATACAAGATGATCACCCACCTTGGCCAGAACTCAACTTACTGGGTTATGAAGATAATTGACTCGAGAAAATTCTCAGAAACTGAATTGGAGAAGATCTTGGATGTGTTTCGGAGTGCTGTTACCGGGTACTTTGATAGCAGGAAGTCTCAGTTGAAGATAGATTTTCTTGAGGAAGTGTTTAGAAGGAGACCATGGATTGGACACCAACTGTTTGGTTTCCTTCTGGAGGCAAGTGTGAATGCAAATGTTGAGTTCCGTAGACTGGAAGCTCTTGATTTGATCACTGAAACTCTGAGATCGTTGATTCCTATAAACGAAAACACCCAAGCAGATTCGAGAAAAACAATGACGACCCATCTGAAGGAACTGATTCTTTTGATTAAAGAACTAGTTGGAAACATGCCTGAGGCTAAAGTAAGGCGAGCTCAGGTGCGTAAATTCTGTGGCAGGATCTTTCAGATGGTCTCTTCGTTGAAACTCACCAAATCTTTCCTCAAGGGGTTGGGACAAGATGGTCGAACAGCATGTGAAGATGCATTCGGTGATCTCTTCTTGAATCTGAAGAATACAGAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1306

Amino Acids

146.11

Weight (kDa)

5.2

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 188 - 1018 9.9e-180 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AasI GACNNNNNNGTC 1 cut(s) 3855
Acc16I TGCGCA 1 cut(s) 610
Acc36I ACCTGC 1 cut(s) 2602
AccB7I CCANNNNNTGG 1 cut(s) 3539
AccI GTMKAC 1 cut(s) 3601
AccII CGCG 2 cut(s) 274, 1642
AclWI GGATC 4 cut(s) 1969, 2183, 2345, 3800
AcoI YGGCCR 3 cut(s) 1587, 2063, 3364
AcuI CTGAAG 8 cut(s) 266, 1704, 1770, 1949, 2046, 2099, 2568, 3722
AfaI GTAC 4 cut(s) 1995, 2624, 3092, 3470
AfeI AGCGCT 1 cut(s) 1035
AfiI CCNNNNNNNGG 6 cut(s) 1051, 2416, 3539, 3568, 3604, 3703
AflIII ACRYGT 1 cut(s) 1453
AhdI GACNNNNNGTC 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 3730
AjnI CCWGG 3 cut(s) 160, 1915, 2172
AjuI GAANNNNNNNTTGG 2 cut(s) 2584, 2616
AleI CACNNNNGTG 1 cut(s) 1004
Alw21I GWGCWC 3 cut(s) 176, 550, 3769
Alw26I GTCTC 8 cut(s) 223, 336, 595, 1356, 2006, 3492, 3523, 3811
AlwI GGATC 4 cut(s) 1969, 2183, 2345, 3800
AlwNI CAGNNNCTG 4 cut(s) 425, 1978, 2435, 3422
Ama87I CYCGRG 1 cut(s) 3403
Aor51HI AGCGCT 1 cut(s) 1035
AoxI GGCC 5 cut(s) 275, 1196, 1587, 2063, 3364
AseI ATTAAT 1 cut(s) 666
Asp700I GAANNNNTTC 5 cut(s) 211, 2112, 3185, 3278, 3711
AspLEI GCGC 3 cut(s) 611, 778, 1036
AspS9I GGNCC 2 cut(s) 1773, 2710
AsuC2I CCSGG 3 cut(s) 1108, 2418, 3466
AsuHPI GGTGA 9 cut(s) 1573, 2519, 2632, 2683, 2804, 2813, 3346, 3814, 3896
AvaI CYCGRG 1 cut(s) 3403
AvaII GGWCC 2 cut(s) 1773, 2710
AxyI CCTNAGG 2 cut(s) 1947, 3747
BaeGI GKGCMC 1 cut(s) 2024
BalI TGGCCA 2 cut(s) 2065, 3366
BanII GRGCYC 2 cut(s) 176, 3769
BarI GAAGNNNNNNTAC 2 cut(s) 1350, 1382
BauI CACGAG 2 cut(s) 1179, 2549
BbsI GAAGAC 2 cut(s) 799, 2592
Bbv12I GWGCWC 3 cut(s) 176, 550, 3769
BceAI ACGGC 2 cut(s) 153, 1574
BciT130I CCWGG 3 cut(s) 162, 1917, 2174
BclI TGATCA 4 cut(s) 1126, 2673, 3351, 3621
BcnI CCSGG 3 cut(s) 1108, 2418, 3466
BcoDI GTCTC 8 cut(s) 223, 336, 595, 1356, 2006, 3492, 3523, 3811
BcuI ACTAGT 1 cut(s) 3730
BfaI CTAG 7 cut(s) 233, 660, 914, 1664, 1778, 2697, 3731
BfmI CTRYAG 2 cut(s) 1668, 3129
BfoI RGCGCY 1 cut(s) 1037
BfuAI ACCTGC 1 cut(s) 2602
BglII AGATCT 3 cut(s) 2683, 3183, 3435
BmcAI AGTACT 1 cut(s) 2624
Bme1390I CCNGG 6 cut(s) 162, 1108, 1917, 2174, 2418, 3466
Bme18I GGWCC 2 cut(s) 1773, 2710
BmeRI GACNNNNNGTC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 3403
BmgT120I GGNCC 2 cut(s) 1773, 2710
BmiI GGNNCC 3 cut(s) 894, 1775, 3338
BmrFI CCNGG 6 cut(s) 162, 1108, 1917, 2174, 2418, 3466
BmrI ACTGGG 1 cut(s) 3391
BmsI GCATC 9 cut(s) 391, 498, 1009, 1929, 2112, 2755, 2911, 3177, 3865
BmuI ACTGGG 1 cut(s) 3391
BpiI GAAGAC 2 cut(s) 799, 2592
BplI GAGNNNNNCTC 2 cut(s) 550, 582
BpmI CTGGAG 4 cut(s) 954, 1589, 1691, 3587
Bpu10I CCTNAGC 3 cut(s) 279, 2216, 3768
BpuEI CTTGAG 2 cut(s) 3530, 3821
BpuMI CCSGG 3 cut(s) 1108, 2418, 3466
BsaI GGTCTC 4 cut(s) 1356, 2006, 3523, 3811
BsaJI CCNNGG 6 cut(s) 1002, 1915, 2172, 3207, 3361, 3533
BsaWI WCCGGW 4 cut(s) 176, 1222, 1290, 1871
BsaXI ACNNNNNCTCC 4 cut(s) 1689, 1719, 2062, 2092
Bsc4I CCNNNNNNNGG 6 cut(s) 1051, 2416, 3539, 3568, 3604, 3703
Bse118I RCCGGY 1 cut(s) 2840
Bse1I ACTGG 6 cut(s) 1091, 1304, 2803, 3270, 3386, 3609
Bse21I CCTNAGG 2 cut(s) 1947, 3747
Bse3DI GCAATG 3 cut(s) 690, 2931, 3117
BseBI CCWGG 3 cut(s) 162, 1917, 2174
BseDI CCNNGG 6 cut(s) 1002, 1915, 2172, 3207, 3361, 3533
BseLI CCNNNNNNNGG 6 cut(s) 1051, 2416, 3539, 3568, 3604, 3703
BseMI GCAATG 3 cut(s) 690, 2931, 3117
BseNI ACTGG 6 cut(s) 1091, 1304, 2803, 3270, 3386, 3609
BseRI GAGGAG 5 cut(s) 241, 540, 1862, 2399, 2798
BseSI GKGCMC 1 cut(s) 2024
BseYI CCCAGC 2 cut(s) 1896, 3003
BsgI GTGCAG 1 cut(s) 3216
Bsh1236I CGCG 2 cut(s) 274, 1642
BshFI GGCC 5 cut(s) 277, 1198, 1589, 2065, 3366
BsiHKAI GWGCWC 3 cut(s) 176, 550, 3769
BsiHKCI CYCGRG 1 cut(s) 3403
BsiSI CCGG 8 cut(s) 177, 1107, 1223, 1291, 1872, 2417, 2841, 3465
BslFI GGGAC 6 cut(s) 741, 1759, 1927, 2834, 2986, 3861
BslI CCNNNNNNNGG 6 cut(s) 1051, 2416, 3539, 3568, 3604, 3703
BsmAI GTCTC 8 cut(s) 223, 336, 595, 1356, 2006, 3492, 3523, 3811
BsmFI GGGAC 6 cut(s) 741, 1759, 1927, 2834, 2986, 3861
BsmI GAATGC 4 cut(s) 749, 2578, 3586, 3878
BsnI GGCC 5 cut(s) 277, 1198, 1589, 2065, 3366
Bso31I GGTCTC 4 cut(s) 1356, 2006, 3523, 3811
BsoBI CYCGRG 1 cut(s) 3403
Bsp1286I GDGCHC 4 cut(s) 176, 550, 2024, 3769
Bsp1407I TGTACA 1 cut(s) 1993
Bsp19I CCATGG 1 cut(s) 3533
BspANI GGCC 5 cut(s) 277, 1198, 1589, 2065, 3366
BspFNI CGCG 2 cut(s) 274, 1642
BspLI GGNNCC 3 cut(s) 894, 1775, 3338
BspMAI CTGCAG 2 cut(s) 1672, 3133
BspMI ACCTGC 1 cut(s) 2602
BspPI GGATC 4 cut(s) 1969, 2183, 2345, 3800
BspTNI GGTCTC 4 cut(s) 1356, 2006, 3523, 3811
BsrDI GCAATG 3 cut(s) 690, 2931, 3117
BsrFI RCCGGY 1 cut(s) 2840
BsrGI TGTACA 1 cut(s) 1993
BsrI ACTGG 6 cut(s) 1091, 1304, 2803, 3270, 3386, 3609
BssAI RCCGGY 1 cut(s) 2840
BssECI CCNNGG 6 cut(s) 1002, 1915, 2172, 3207, 3361, 3533
BssSI CACGAG 2 cut(s) 1179, 2549
BssT1I CCWWGG 3 cut(s) 3207, 3361, 3533
Bst2BI CACGAG 2 cut(s) 1179, 2549
Bst2UI CCWGG 3 cut(s) 162, 1917, 2174
Bst4CI ACNGT 6 cut(s) 115, 545, 1654, 2694, 3088, 3552
Bst6I CTCTTC 7 cut(s) 8, 339, 598, 2255, 2963, 3814, 3896
BstAUI TGTACA 1 cut(s) 1993
BstC8I GCNNGC 8 cut(s) 172, 575, 780, 1082, 1175, 1391, 2983, 3765
BstDSI CCRYGG 1 cut(s) 3533
BstENI CCTNNNNNAGG 1 cut(s) 3566
BstFNI CGCG 2 cut(s) 274, 1642
BstH2I RGCGCY 1 cut(s) 1037
BstHHI GCGC 3 cut(s) 611, 778, 1036
BstMAI GTCTC 8 cut(s) 223, 336, 595, 1356, 2006, 3492, 3523, 3811
BstMWI GCNNNNNNNGC 8 cut(s) 146, 149, 280, 773, 886, 1033, 2081, 2100
BstNI CCWGG 3 cut(s) 162, 1917, 2174
BstNSI RCATGY 5 cut(s) 1457, 1745, 1934, 3745, 3870
BstSCI CCNGG 6 cut(s) 160, 1106, 1915, 2172, 2416, 3464
BstSFI CTRYAG 2 cut(s) 1668, 3129
BstSLI GKGCMC 1 cut(s) 2024
BstUI CGCG 2 cut(s) 274, 1642
BstV2I GAAGAC 2 cut(s) 799, 2592
BstX2I RGATCY 5 cut(s) 1974, 2683, 3183, 3435, 3792
BstXI CCANNNNNNTGG 3 cut(s) 1006, 1585, 3554
BstYI RGATCY 5 cut(s) 1974, 2683, 3183, 3435, 3792
Bsu36I CCTNAGG 2 cut(s) 1947, 3747
BsuRI GGCC 5 cut(s) 277, 1198, 1589, 2065, 3366
BtgI CCRYGG 1 cut(s) 3533
BtgZI GCGATG 1 cut(s) 2823
BtsI GCAGTG 2 cut(s) 1134, 3126
BtsIMutI CAGTG 6 cut(s) 1134, 1484, 2022, 3093, 3126, 3624
BveI ACCTGC 1 cut(s) 2602
Cac8I GCNNGC 8 cut(s) 172, 575, 780, 1082, 1175, 1391, 2983, 3765
CaiI CAGNNNCTG 4 cut(s) 425, 1978, 2435, 3422
CfoI GCGC 3 cut(s) 611, 778, 1036
Cfr10I RCCGGY 1 cut(s) 2840
Cfr13I GGNCC 2 cut(s) 1773, 2710
Csp6I GTAC 4 cut(s) 1994, 2623, 3091, 3469
CviQI GTAC 4 cut(s) 1994, 2623, 3091, 3469
DraI TTTAAA 2 cut(s) 2319, 3019
DrdI GACNNNNNNGTC 1 cut(s) 3855
DriI GACNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 3855
EaeI YGGCCR 3 cut(s) 1587, 2063, 3364
Eam1104I CTCTTC 7 cut(s) 8, 339, 598, 2255, 2963, 3814, 3896
Eam1105I GACNNNNNGTC 1 cut(s) 328
EarI CTCTTC 7 cut(s) 8, 339, 598, 2255, 2963, 3814, 3896
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 2 cut(s) 174, 3767
Eco130I CCWWGG 3 cut(s) 3207, 3361, 3533
Eco24I GRGCYC 2 cut(s) 176, 3769
Eco31I GGTCTC 4 cut(s) 1356, 2006, 3523, 3811
Eco32I GATATC 1 cut(s) 1408
Eco47I GGWCC 2 cut(s) 1773, 2710
Eco47III AGCGCT 1 cut(s) 1035
Eco53kI GAGCTC 2 cut(s) 174, 3767
Eco57I CTGAAG 8 cut(s) 266, 1704, 1770, 1949, 2046, 2099, 2568, 3722
Eco81I CCTNAGG 2 cut(s) 1947, 3747
Eco88I CYCGRG 1 cut(s) 3403
EcoICRI GAGCTC 2 cut(s) 174, 3767
EcoNI CCTNNNNNAGG 1 cut(s) 3566
EcoRI GAATTC 2 cut(s) 1795, 2423
EcoRII CCWGG 3 cut(s) 160, 1915, 2172
EcoRV GATATC 1 cut(s) 1408
EcoT14I CCWWGG 3 cut(s) 3207, 3361, 3533
EcoT22I ATGCAT 2 cut(s) 2371, 3880
EcoT38I GRGCYC 2 cut(s) 176, 3769
ErhI CCWWGG 3 cut(s) 3207, 3361, 3533
FalI AAGNNNNNCTT 8 cut(s) 731, 763, 869, 901, 2736, 2768, 3170, 3202
FaqI GGGAC 6 cut(s) 741, 1759, 1927, 2834, 2986, 3861
FauI CCCGC 1 cut(s) 2661
FbaI TGATCA 4 cut(s) 1126, 2673, 3351, 3621
FblI GTMKAC 1 cut(s) 3601
FriOI GRGCYC 2 cut(s) 176, 3769
FspBI CTAG 7 cut(s) 233, 660, 914, 1664, 1778, 2697, 3731
FspI TGCGCA 1 cut(s) 610
GlaI GCGC 3 cut(s) 610, 777, 1035
GsaI CCCAGC 2 cut(s) 1900, 3007
GsuI CTGGAG 4 cut(s) 954, 1589, 1691, 3587
HaeII RGCGCY 1 cut(s) 1037
HaeIII GGCC 5 cut(s) 277, 1198, 1589, 2065, 3366
HapII CCGG 8 cut(s) 177, 1107, 1223, 1291, 1872, 2417, 2841, 3465
HhaI GCGC 3 cut(s) 611, 778, 1036
Hin6I GCGC 3 cut(s) 609, 776, 1034
HinP1I GCGC 3 cut(s) 609, 776, 1034
HincII GTYRAC 4 cut(s) 636, 925, 2863, 3304
HindII GTYRAC 4 cut(s) 636, 925, 2863, 3304
HindIII AAGCTT 3 cut(s) 210, 1430, 2466
HpaII CCGG 8 cut(s) 177, 1107, 1223, 1291, 1872, 2417, 2841, 3465
HphI GGTGA 9 cut(s) 1573, 2519, 2632, 2683, 2804, 2813, 3346, 3814, 3896
Hpy166II GTNNAC 9 cut(s) 636, 925, 2312, 2339, 2554, 2863, 3091, 3304, 3602
Hpy8I GTNNAC 9 cut(s) 636, 925, 2312, 2339, 2554, 2863, 3091, 3304, 3602
Hpy99I CGWCG 4 cut(s) 29, 37, 326, 329
HpyAV CCTTC 9 cut(s) 1150, 1352, 1613, 1846, 3047, 3318, 3519, 3572, 3697
HpyCH4III ACNGT 6 cut(s) 115, 545, 1654, 2694, 3088, 3552
HpyCH4IV ACGT 4 cut(s) 784, 1285, 2222, 3157
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 149, 280, 773, 886, 1033, 2081, 2100
HpySE526I ACGT 4 cut(s) 784, 1285, 2222, 3157
HspAI GCGC 3 cut(s) 609, 776, 1034
Ksp22I TGATCA 4 cut(s) 1126, 2673, 3351, 3621
LmnI GCTCC 5 cut(s) 179, 266, 553, 973, 2593
LweI GCATC 9 cut(s) 391, 498, 1009, 1929, 2112, 2755, 2911, 3177, 3865
MaeI CTAG 7 cut(s) 233, 660, 914, 1664, 1778, 2697, 3731
MaeII ACGT 4 cut(s) 784, 1285, 2222, 3157
MfeI CAATTG 1 cut(s) 72
MflI RGATCY 5 cut(s) 1974, 2683, 3183, 3435, 3792
MhlI GDGCHC 4 cut(s) 176, 550, 2024, 3769
MlsI TGGCCA 2 cut(s) 2065, 3366
MluNI TGGCCA 2 cut(s) 2065, 3366
MlyI GAGTC 9 cut(s) 23, 338, 437, 597, 818, 1502, 1816, 2711, 3395
MmeI TCCRAC 4 cut(s) 1474, 1843, 3228, 3715
Mox20I TGGCCA 2 cut(s) 2065, 3366
Mph1103I ATGCAT 2 cut(s) 2371, 3880
MroXI GAANNNNTTC 5 cut(s) 211, 2112, 3185, 3278, 3711
MscI TGGCCA 2 cut(s) 2065, 3366
MslI CAYNNNNRTG 2 cut(s) 668, 1004
Msp20I TGGCCA 2 cut(s) 2065, 3366
MspA1I CMGCKG 5 cut(s) 422, 899, 2119, 2392, 3329
MspI CCGG 8 cut(s) 177, 1107, 1223, 1291, 1872, 2417, 2841, 3465
MspR9I CCNGG 6 cut(s) 162, 1108, 1917, 2174, 2418, 3466
MssI GTTTAAAC 1 cut(s) 3019
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 4 cut(s) 749, 2578, 3586, 3878
MvaI CCWGG 3 cut(s) 162, 1917, 2174
MvnI CGCG 2 cut(s) 274, 1642
MwoI GCNNNNNNNGC 8 cut(s) 146, 149, 280, 773, 886, 1033, 2081, 2100
NciI CCSGG 3 cut(s) 1108, 2418, 3466
NcoI CCATGG 1 cut(s) 3533
NlaIV GGNNCC 3 cut(s) 894, 1775, 3338
NmuCI GTSAC 2 cut(s) 2459, 2638
NsbI TGCGCA 1 cut(s) 610
NsiI ATGCAT 2 cut(s) 2371, 3880
NspI RCATGY 5 cut(s) 1457, 1745, 1934, 3745, 3870
OliI CACNNNNGTG 1 cut(s) 1004
PaeR7I CTCGAG 1 cut(s) 3403
PciI ACATGT 1 cut(s) 1453
PctI GAATGC 4 cut(s) 749, 2578, 3586, 3878
PdmI GAANNNNTTC 5 cut(s) 211, 2112, 3185, 3278, 3711
PflMI CCANNNNNTGG 1 cut(s) 3539
PfoI TCCNGGA 2 cut(s) 1106, 2416
PleI GAGTC 9 cut(s) 23, 337, 436, 596, 818, 1502, 1815, 2711, 3395
PmeI GTTTAAAC 1 cut(s) 3019
PpsI GAGTC 9 cut(s) 23, 337, 436, 596, 818, 1502, 1815, 2711, 3395
PscI ACATGT 1 cut(s) 1453
PshBI ATTAAT 1 cut(s) 666
PsiI TTATAA 1 cut(s) 981
Psp124BI GAGCTC 2 cut(s) 176, 3769
Psp6I CCWGG 3 cut(s) 160, 1915, 2172
PspFI CCCAGC 2 cut(s) 1896, 3003
PspGI CCWGG 3 cut(s) 160, 1915, 2172
PspN4I GGNNCC 3 cut(s) 894, 1775, 3338
PspPI GGNCC 2 cut(s) 1773, 2710
PsrI GAACNNNNNNTAC 2 cut(s) 3362, 3394
PstI CTGCAG 2 cut(s) 1672, 3133
PstNI CAGNNNCTG 4 cut(s) 425, 1978, 2435, 3422
PsuI RGATCY 5 cut(s) 1974, 2683, 3183, 3435, 3792
PvuII CAGCTG 5 cut(s) 422, 899, 2119, 2392, 3329
RsaI GTAC 4 cut(s) 1995, 2624, 3092, 3470
RsaNI GTAC 4 cut(s) 1994, 2623, 3091, 3469
RseI CAYNNNNRTG 2 cut(s) 668, 1004
SacI GAGCTC 2 cut(s) 176, 3769
Sau96I GGNCC 2 cut(s) 1773, 2710
ScaI AGTACT 1 cut(s) 2624
SchI GAGTC 9 cut(s) 23, 338, 437, 597, 818, 1502, 1816, 2711, 3395
ScrFI CCNGG 6 cut(s) 162, 1108, 1917, 2174, 2418, 3466
SduI GDGCHC 4 cut(s) 176, 550, 2024, 3769
SfaNI GCATC 9 cut(s) 391, 498, 1009, 1929, 2112, 2755, 2911, 3177, 3865
SfcI CTRYAG 2 cut(s) 1668, 3129
Sfr274I CTCGAG 1 cut(s) 3403
SinI GGWCC 2 cut(s) 1773, 2710
SlaI CTCGAG 1 cut(s) 3403
SmiMI CAYNNNNRTG 2 cut(s) 668, 1004
SmlI CTYRAG 3 cut(s) 3403, 3509, 3836
SmoI CTYRAG 3 cut(s) 3403, 3509, 3836
SpeI ACTAGT 1 cut(s) 3730
SspI AATATT 2 cut(s) 1636, 2233
SspMI CTAG 7 cut(s) 233, 660, 914, 1664, 1778, 2697, 3731
SstI GAGCTC 2 cut(s) 176, 3769
StyD4I CCNGG 6 cut(s) 160, 1106, 1915, 2172, 2416, 3464
StyI CCWWGG 3 cut(s) 3207, 3361, 3533
TaaI ACNGT 6 cut(s) 115, 545, 1654, 2694, 3088, 3552
TaiI ACGT 4 cut(s) 787, 1288, 2225, 3160
TatI WGTACW 3 cut(s) 1993, 2622, 3090
TauI GCSGC 9 cut(s) 143, 146, 149, 152, 277, 350, 2578, 2988, 3148
TscAI CASTG 6 cut(s) 1141, 1484, 2029, 3093, 3133, 3631
TseFI GTSAC 2 cut(s) 2459, 2638
Tsp45I GTSAC 2 cut(s) 2459, 2638
TspGWI ACGGA 6 cut(s) 50, 397, 1832, 2243, 3014, 3587
TspRI CASTG 6 cut(s) 1141, 1484, 2029, 3093, 3133, 3631
Van91I CCANNNNNTGG 1 cut(s) 3539
VpaK11BI GGWCC 2 cut(s) 1773, 2710
VspI ATTAAT 1 cut(s) 666
XagI CCTNNNNNAGG 1 cut(s) 3566
XbaI TCTAGA 2 cut(s) 913, 2696
XceI RCATGY 5 cut(s) 1457, 1745, 1934, 3745, 3870
XhoI CTCGAG 1 cut(s) 3403
XmiI GTMKAC 1 cut(s) 3601
XmnI GAANNNNTTC 5 cut(s) 211, 2112, 3185, 3278, 3711
XspI CTAG 7 cut(s) 233, 660, 914, 1664, 1778, 2697, 3731
ZrmI AGTACT 1 cut(s) 2624
Zsp2I ATGCAT 2 cut(s) 2371, 3880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.