DNA polymerase
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G64420 |
| fragaria_vesca | FvH4_2g11160 |
| malus_domestica | MD05G1049800.v1.1 MD10G1056900.v1.1 |
| prunus_persica | Prupe.8G075100_v2.0.a1 |
| pyrus_communis | pycom05g04160 pycom05g04170 pycom10g04380 |
| rosa_chinensis | RchiOBHm_Chr6g0269481 |
| rosa_laevigata | RLG00000013848 RLG00000013849 |
| rosa_multiflora | Rmu_ssc0000291.1_g000033 |
| rosa_roxburghii | Rroxscaffold_7G00198700 |
| rosa_rugosa | Rorug06G0048500 |
| rosa_samantha | Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100 |
| rosa_wichuraiana | Rw6G014500 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 562, 2907 |
| Acc36I | ACCTGC | 3 cut(s) | 2506, 2605, 3225 |
| Acc65I | GGTACC | 1 cut(s) | 277 |
| AccB1I | GGYRCC | 2 cut(s) | 277, 3453 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 3291 |
| AccBSI | CCGCTC | 1 cut(s) | 784 |
| AccII | CGCG | 1 cut(s) | 3685 |
| AccIII | TCCGGA | 1 cut(s) | 2841 |
| AclI | AACGTT | 1 cut(s) | 2631 |
| AclWI | GGATC | 5 cut(s) | 702, 1040, 1308, 1321, 1620 |
| AcoI | YGGCCR | 3 cut(s) | 1976, 3440, 3574 |
| AcvI | CACGTG | 1 cut(s) | 2069 |
| AdeI | CACNNNGTG | 2 cut(s) | 1144, 2136 |
| AfaI | GTAC | 5 cut(s) | 279, 604, 1137, 2111, 3002 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 3291, 3449 |
| AflII | CTTAAG | 1 cut(s) | 3725 |
| AflIII | ACRYGT | 1 cut(s) | 1674 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1084 |
| AhlI | ACTAGT | 1 cut(s) | 800 |
| AjnI | CCWGG | 8 cut(s) | 124, 922, 1828, 2401, 2974, 3242, 3552, 3576 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 425, 457, 3158, 3190, 3774, 3806 |
| Alw21I | GWGCWC | 3 cut(s) | 730, 2002, 3284 |
| Alw26I | GTCTC | 9 cut(s) | 163, 196, 215, 520, 1027, 2353, 2690, 3323, 3402 |
| AlwI | GGATC | 5 cut(s) | 702, 1040, 1308, 1321, 1620 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 299, 2351, 3332 |
| Ama87I | CYCGRG | 1 cut(s) | 1993 |
| Aor13HI | TCCGGA | 1 cut(s) | 2841 |
| ApeKI | GCWGC | 9 cut(s) | 41, 371, 1073, 1985, 2023, 2302, 2476, 3209, 3337 |
| AseI | ATTAAT | 1 cut(s) | 3807 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1537, 2378 |
| Asp718I | GGTACC | 1 cut(s) | 277 |
| AspLEI | GCGC | 4 cut(s) | 502, 563, 2908, 3685 |
| AspS9I | GGNCC | 4 cut(s) | 128, 143, 919, 2063 |
| AsuC2I | CCSGG | 1 cut(s) | 3362 |
| AsuHPI | GGTGA | 9 cut(s) | 68, 634, 1312, 2435, 2714, 2723, 2903, 3134, 3544 |
| AvaI | CYCGRG | 1 cut(s) | 1993 |
| AvaII | GGWCC | 1 cut(s) | 143 |
| AxyI | CCTNAGG | 1 cut(s) | 3117 |
| BaeGI | GKGCMC | 1 cut(s) | 3456 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 261, 294 |
| BamHI | GGATCC | 1 cut(s) | 1313 |
| BanI | GGYRCC | 2 cut(s) | 277, 3453 |
| BanII | GRGCYC | 1 cut(s) | 3284 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1152, 1184 |
| BauI | CACGAG | 1 cut(s) | 1131 |
| BbrPI | CACGTG | 1 cut(s) | 2069 |
| BbsI | GAAGAC | 7 cut(s) | 657, 751, 797, 1080, 1407, 1767, 2193 |
| Bbv12I | GWGCWC | 3 cut(s) | 730, 2002, 3284 |
| BbvCI | CCTCAGC | 1 cut(s) | 753 |
| BbvI | GCAGC | 9 cut(s) | 53, 358, 1060, 1997, 2035, 2289, 2463, 3221, 3324 |
| BceAI | ACGGC | 1 cut(s) | 2750 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1253, 1287, 1974, 2008 |
| BciT130I | CCWGG | 8 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3554, 3578 |
| BciVI | GTATCC | 1 cut(s) | 2452 |
| BcnI | CCSGG | 1 cut(s) | 3362 |
| BcoDI | GTCTC | 9 cut(s) | 163, 196, 215, 520, 1027, 2353, 2690, 3323, 3402 |
| BcuI | ACTAGT | 1 cut(s) | 800 |
| BfaI | CTAG | 8 cut(s) | 749, 801, 914, 1251, 1901, 2601, 2951, 3371 |
| BfmI | CTRYAG | 4 cut(s) | 3039, 3207, 3338, 3611 |
| BfrI | CTTAAG | 1 cut(s) | 3725 |
| BfuAI | ACCTGC | 3 cut(s) | 2506, 2605, 3225 |
| BfuI | GTATCC | 1 cut(s) | 2452 |
| Bme1390I | CCNGG | 9 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3362, 3554, 3578 |
| Bme18I | GGWCC | 1 cut(s) | 143 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1084 |
| BmeT110I | CYCGRG | 1 cut(s) | 1993 |
| BmgT120I | GGNCC | 4 cut(s) | 128, 143, 919, 2063 |
| BmrFI | CCNGG | 9 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3362, 3554, 3578 |
| BmrI | ACTGGG | 1 cut(s) | 309 |
| BmuI | ACTGGG | 1 cut(s) | 309 |
| BpiI | GAAGAC | 7 cut(s) | 657, 751, 797, 1080, 1407, 1767, 2193 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2732, 2764 |
| BpmI | CTGGAG | 3 cut(s) | 7, 2012, 2424 |
| Bpu10I | CCTNAGC | 2 cut(s) | 753, 3015 |
| BpuEI | CTTGAG | 3 cut(s) | 449, 535, 2108 |
| BpuMI | CCSGG | 1 cut(s) | 3362 |
| BsaAI | YACGTR | 1 cut(s) | 2069 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2430 |
| BsaI | GGTCTC | 1 cut(s) | 215 |
| BsaWI | WCCGGW | 1 cut(s) | 2841 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 3291, 3449 |
| Bse1I | ACTGG | 6 cut(s) | 24, 145, 304, 1285, 2029, 3528 |
| Bse21I | CCTNAGG | 1 cut(s) | 3117 |
| Bse3DI | GCAATG | 6 cut(s) | 629, 810, 1209, 1576, 2462, 2471 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2430 |
| BseAI | TCCGGA | 1 cut(s) | 2841 |
| BseBI | CCWGG | 8 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3554, 3578 |
| BseJI | GATNNNNATC | 1 cut(s) | 2430 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 3291, 3449 |
| BseMI | GCAATG | 6 cut(s) | 629, 810, 1209, 1576, 2462, 2471 |
| BseNI | ACTGG | 6 cut(s) | 24, 145, 304, 1285, 2029, 3528 |
| BseRI | GAGGAG | 1 cut(s) | 770 |
| BseSI | GKGCMC | 1 cut(s) | 3456 |
| BseXI | GCAGC | 9 cut(s) | 53, 358, 1060, 1997, 2035, 2289, 2463, 3221, 3324 |
| BseYI | CCCAGC | 2 cut(s) | 611, 2891 |
| BsgI | GTGCAG | 1 cut(s) | 428 |
| Bsh1236I | CGCG | 1 cut(s) | 3685 |
| BshNI | GGYRCC | 2 cut(s) | 277, 3453 |
| BsiHKAI | GWGCWC | 3 cut(s) | 730, 2002, 3284 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1993 |
| BsiSI | CCGG | 3 cut(s) | 311, 2842, 3361 |
| BslFI | GGGAC | 3 cut(s) | 129, 2639, 3535 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 3291, 3449 |
| BsmAI | GTCTC | 9 cut(s) | 163, 196, 215, 520, 1027, 2353, 2690, 3323, 3402 |
| BsmBI | CGTCTC | 1 cut(s) | 196 |
| BsmFI | GGGAC | 3 cut(s) | 129, 2639, 3535 |
| BsmI | GAATGC | 1 cut(s) | 2854 |
| Bso31I | GGTCTC | 1 cut(s) | 215 |
| BsoBI | CYCGRG | 1 cut(s) | 1993 |
| Bsp1286I | GDGCHC | 4 cut(s) | 730, 2002, 3284, 3456 |
| Bsp13I | TCCGGA | 1 cut(s) | 2841 |
| Bsp1407I | TGTACA | 1 cut(s) | 1135 |
| Bsp143I | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| Bsp19I | CCATGG | 4 cut(s) | 30, 162, 1782, 3443 |
| BspEI | TCCGGA | 1 cut(s) | 2841 |
| BspFNI | CGCG | 1 cut(s) | 3685 |
| BspHI | TCATGA | 2 cut(s) | 931, 1333 |
| BspMAI | CTGCAG | 3 cut(s) | 3043, 3211, 3342 |
| BspMI | ACCTGC | 3 cut(s) | 2506, 2605, 3225 |
| BspPI | GGATC | 5 cut(s) | 702, 1040, 1308, 1321, 1620 |
| BspQI | GCTCTTC | 1 cut(s) | 1261 |
| BspT107I | GGYRCC | 2 cut(s) | 277, 3453 |
| BspTI | CTTAAG | 1 cut(s) | 3725 |
| BspTNI | GGTCTC | 1 cut(s) | 215 |
| BsrBI | CCGCTC | 1 cut(s) | 784 |
| BsrDI | GCAATG | 6 cut(s) | 629, 810, 1209, 1576, 2462, 2471 |
| BsrGI | TGTACA | 1 cut(s) | 1135 |
| BsrI | ACTGG | 6 cut(s) | 24, 145, 304, 1285, 2029, 3528 |
| BssMI | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| BssSI | CACGAG | 1 cut(s) | 1131 |
| BssT1I | CCWWGG | 5 cut(s) | 30, 162, 1379, 1782, 3443 |
| Bst2BI | CACGAG | 1 cut(s) | 1131 |
| Bst2UI | CCWGG | 8 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3554, 3578 |
| Bst4CI | ACNGT | 4 cut(s) | 1019, 1606, 2484, 3234 |
| Bst6I | CTCTTC | 3 cut(s) | 213, 1260, 1261 |
| BstAFI | CTTAAG | 1 cut(s) | 3725 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 47, 2136 |
| BstAUI | TGTACA | 1 cut(s) | 1135 |
| BstBAI | YACGTR | 1 cut(s) | 2069 |
| BstC8I | GCNNGC | 4 cut(s) | 1191, 1389, 1649, 2277 |
| BstDSI | CCRYGG | 4 cut(s) | 30, 162, 1782, 3443 |
| BstFNI | CGCG | 1 cut(s) | 3685 |
| BstHHI | GCGC | 4 cut(s) | 502, 563, 2908, 3685 |
| BstKTI | GATC | 8 cut(s) | 697, 740, 1048, 1316, 1615, 1717, 2101, 2389 |
| BstMAI | GTCTC | 9 cut(s) | 163, 196, 215, 520, 1027, 2353, 2690, 3323, 3402 |
| BstMBI | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| BstNI | CCWGG | 8 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3554, 3578 |
| BstNSI | RCATGY | 3 cut(s) | 1651, 1678, 2279 |
| BstSCI | CCNGG | 9 cut(s) | 124, 922, 1828, 2401, 2974, 3242, 3360, 3552, 3576 |
| BstSFI | CTRYAG | 4 cut(s) | 3039, 3207, 3338, 3611 |
| BstSLI | GKGCMC | 1 cut(s) | 3456 |
| BstUI | CGCG | 1 cut(s) | 3685 |
| BstV1I | GCAGC | 9 cut(s) | 53, 358, 1060, 1997, 2035, 2289, 2463, 3221, 3324 |
| BstV2I | GAAGAC | 7 cut(s) | 657, 751, 797, 1080, 1407, 1767, 2193 |
| BstX2I | RGATCY | 3 cut(s) | 1045, 1313, 1612 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 31, 163, 253, 3464 |
| BstYI | RGATCY | 3 cut(s) | 1045, 1313, 1612 |
| Bsu36I | CCTNAGG | 1 cut(s) | 3117 |
| BsuI | GTATCC | 1 cut(s) | 2452 |
| BtgI | CCRYGG | 4 cut(s) | 30, 162, 1782, 3443 |
| BtgZI | GCGATG | 1 cut(s) | 2274 |
| BtsI | GCAGTG | 2 cut(s) | 1920, 2901 |
| BtsIMutI | CAGTG | 9 cut(s) | 213, 1024, 1292, 1436, 1920, 1935, 2901, 3521, 3561 |
| BveI | ACCTGC | 3 cut(s) | 2506, 2605, 3225 |
| Cac8I | GCNNGC | 4 cut(s) | 1191, 1389, 1649, 2277 |
| CaiI | CAGNNNCTG | 3 cut(s) | 299, 2351, 3332 |
| CciI | TCATGA | 2 cut(s) | 931, 1333 |
| CfoI | GCGC | 4 cut(s) | 502, 563, 2908, 3685 |
| Cfr13I | GGNCC | 4 cut(s) | 128, 143, 919, 2063 |
| Csp6I | GTAC | 5 cut(s) | 278, 603, 1136, 2110, 3001 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 2055, 2090, 2973, 3008 |
| CviQI | GTAC | 5 cut(s) | 278, 603, 1136, 2110, 3001 |
| DpnI | GATC | 8 cut(s) | 696, 739, 1047, 1315, 1614, 1716, 2100, 2388 |
| DpnII | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| DraI | TTTAAA | 4 cut(s) | 78, 334, 2524, 3100 |
| DraIII | CACNNNGTG | 2 cut(s) | 1144, 2136 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1084 |
| EaeI | YGGCCR | 3 cut(s) | 1976, 3440, 3574 |
| Eam1104I | CTCTTC | 3 cut(s) | 213, 1260, 1261 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1084 |
| EarI | CTCTTC | 3 cut(s) | 213, 1260, 1261 |
| EciI | GGCGGA | 1 cut(s) | 392 |
| Ecl136II | GAGCTC | 1 cut(s) | 3282 |
| Eco130I | CCWWGG | 5 cut(s) | 30, 162, 1379, 1782, 3443 |
| Eco24I | GRGCYC | 1 cut(s) | 3284 |
| Eco31I | GGTCTC | 1 cut(s) | 215 |
| Eco32I | GATATC | 1 cut(s) | 2542 |
| Eco47I | GGWCC | 1 cut(s) | 143 |
| Eco53kI | GAGCTC | 1 cut(s) | 3282 |
| Eco72I | CACGTG | 1 cut(s) | 2069 |
| Eco81I | CCTNAGG | 1 cut(s) | 3117 |
| Eco88I | CYCGRG | 1 cut(s) | 1993 |
| EcoICRI | GAGCTC | 1 cut(s) | 3282 |
| EcoO109I | RGGNCCY | 1 cut(s) | 919 |
| EcoRI | GAATTC | 2 cut(s) | 794, 3078 |
| EcoRII | CCWGG | 8 cut(s) | 124, 922, 1828, 2401, 2974, 3242, 3552, 3576 |
| EcoRV | GATATC | 1 cut(s) | 2542 |
| EcoT14I | CCWWGG | 5 cut(s) | 30, 162, 1379, 1782, 3443 |
| EcoT22I | ATGCAT | 1 cut(s) | 2281 |
| EcoT38I | GRGCYC | 1 cut(s) | 3284 |
| ErhI | CCWWGG | 5 cut(s) | 30, 162, 1379, 1782, 3443 |
| Esp3I | CGTCTC | 1 cut(s) | 196 |
| FaqI | GGGAC | 3 cut(s) | 129, 2639, 3535 |
| FriOI | GRGCYC | 1 cut(s) | 3284 |
| FspBI | CTAG | 8 cut(s) | 749, 801, 914, 1251, 1901, 2601, 2951, 3371 |
| FspI | TGCGCA | 2 cut(s) | 562, 2907 |
| GlaI | GCGC | 4 cut(s) | 501, 562, 2907, 3684 |
| GsaI | CCCAGC | 2 cut(s) | 615, 2895 |
| GsuI | CTGGAG | 3 cut(s) | 7, 2012, 2424 |
| HapII | CCGG | 3 cut(s) | 311, 2842, 3361 |
| HhaI | GCGC | 4 cut(s) | 502, 563, 2908, 3685 |
| Hin6I | GCGC | 4 cut(s) | 500, 561, 2906, 3683 |
| HinP1I | GCGC | 4 cut(s) | 500, 561, 2906, 3683 |
| HincII | GTYRAC | 1 cut(s) | 3645 |
| HindII | GTYRAC | 1 cut(s) | 3645 |
| HindIII | AAGCTT | 5 cut(s) | 186, 381, 2376, 2796, 2930 |
| HpaII | CCGG | 3 cut(s) | 311, 2842, 3361 |
| HphI | GGTGA | 9 cut(s) | 68, 634, 1312, 2435, 2714, 2723, 2903, 3134, 3544 |
| Hpy166II | GTNNAC | 8 cut(s) | 625, 1136, 1138, 1162, 1920, 3001, 3053, 3645 |
| Hpy8I | GTNNAC | 8 cut(s) | 625, 1136, 1138, 1162, 1920, 3001, 3053, 3645 |
| Hpy99I | CGWCG | 2 cut(s) | 2645, 3513 |
| HpyCH4III | ACNGT | 4 cut(s) | 1019, 1606, 2484, 3234 |
| HpyCH4IV | ACGT | 3 cut(s) | 205, 2068, 2631 |
| HpySE526I | ACGT | 3 cut(s) | 205, 2068, 2631 |
| HspAI | GCGC | 4 cut(s) | 500, 561, 2906, 3683 |
| Kpn2I | TCCGGA | 1 cut(s) | 2841 |
| KpnI | GGTACC | 1 cut(s) | 281 |
| Kzo9I | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| LguI | GCTCTTC | 1 cut(s) | 1261 |
| LmnI | GCTCC | 8 cut(s) | 26, 127, 947, 2031, 2322, 2497, 3133, 3287 |
| Lsp1109I | GCAGC | 9 cut(s) | 53, 358, 1060, 1997, 2035, 2289, 2463, 3221, 3324 |
| MaeI | CTAG | 8 cut(s) | 749, 801, 914, 1251, 1901, 2601, 2951, 3371 |
| MaeII | ACGT | 3 cut(s) | 205, 2068, 2631 |
| MaeIII | GTNAC | 2 cut(s) | 206, 2636 |
| MalI | GATC | 8 cut(s) | 696, 739, 1047, 1315, 1614, 1716, 2100, 2388 |
| MbiI | CCGCTC | 1 cut(s) | 784 |
| MboI | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| MfeI | CAATTG | 1 cut(s) | 3638 |
| MflI | RGATCY | 3 cut(s) | 1045, 1313, 1612 |
| MhlI | GDGCHC | 4 cut(s) | 730, 2002, 3284, 3456 |
| MlyI | GAGTC | 6 cut(s) | 522, 1191, 1729, 2729, 2741, 2816 |
| MmeI | TCCRAC | 4 cut(s) | 444, 940, 1756, 3532 |
| Mph1103I | ATGCAT | 1 cut(s) | 2281 |
| MroI | TCCGGA | 1 cut(s) | 2841 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1537, 2378 |
| MslI | CAYNNNNRTG | 5 cut(s) | 251, 324, 620, 1569, 1679 |
| MspA1I | CMGCKG | 2 cut(s) | 44, 2302 |
| MspCI | CTTAAG | 1 cut(s) | 3725 |
| MspI | CCGG | 3 cut(s) | 311, 2842, 3361 |
| MspR9I | CCNGG | 9 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3362, 3554, 3578 |
| MunI | CAATTG | 1 cut(s) | 3638 |
| Mva1269I | GAATGC | 1 cut(s) | 2854 |
| MvaI | CCWGG | 8 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3554, 3578 |
| MvnI | CGCG | 1 cut(s) | 3685 |
| NciI | CCSGG | 1 cut(s) | 3362 |
| NcoI | CCATGG | 4 cut(s) | 30, 162, 1782, 3443 |
| NdeII | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| NmuCI | GTSAC | 1 cut(s) | 2636 |
| NsbI | TGCGCA | 2 cut(s) | 562, 2907 |
| NsiI | ATGCAT | 1 cut(s) | 2281 |
| NspI | RCATGY | 3 cut(s) | 1651, 1678, 2279 |
| PaeI | GCATGC | 2 cut(s) | 1651, 2279 |
| PaeR7I | CTCGAG | 1 cut(s) | 1993 |
| PagI | TCATGA | 2 cut(s) | 931, 1333 |
| PasI | CCCWGGG | 1 cut(s) | 3553 |
| PciI | ACATGT | 1 cut(s) | 1674 |
| PciSI | GCTCTTC | 1 cut(s) | 1261 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 2637, 2649 |
| PctI | GAATGC | 1 cut(s) | 2854 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1537, 2378 |
| PflFI | GACNNNGTC | 1 cut(s) | 3736 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 3291 |
| PfoI | TCCNGGA | 2 cut(s) | 2401, 3242 |
| PleI | GAGTC | 6 cut(s) | 522, 1190, 1728, 2729, 2741, 2815 |
| PmaCI | CACGTG | 1 cut(s) | 2069 |
| PmlI | CACGTG | 1 cut(s) | 2069 |
| PpsI | GAGTC | 6 cut(s) | 522, 1190, 1728, 2729, 2741, 2815 |
| Ppu21I | YACGTR | 1 cut(s) | 2069 |
| PscI | ACATGT | 1 cut(s) | 1674 |
| PshBI | ATTAAT | 1 cut(s) | 3807 |
| Psp124BI | GAGCTC | 1 cut(s) | 3284 |
| Psp1406I | AACGTT | 1 cut(s) | 2631 |
| Psp6I | CCWGG | 8 cut(s) | 124, 922, 1828, 2401, 2974, 3242, 3552, 3576 |
| PspCI | CACGTG | 1 cut(s) | 2069 |
| PspFI | CCCAGC | 2 cut(s) | 611, 2891 |
| PspGI | CCWGG | 8 cut(s) | 124, 922, 1828, 2401, 2974, 3242, 3552, 3576 |
| PspPI | GGNCC | 4 cut(s) | 128, 143, 919, 2063 |
| PspXI | VCTCGAGB | 1 cut(s) | 1993 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2433, 2465 |
| PstI | CTGCAG | 3 cut(s) | 3043, 3211, 3342 |
| PstNI | CAGNNNCTG | 3 cut(s) | 299, 2351, 3332 |
| PsuI | RGATCY | 3 cut(s) | 1045, 1313, 1612 |
| PsyI | GACNNNGTC | 1 cut(s) | 3736 |
| PvuII | CAGCTG | 2 cut(s) | 44, 2302 |
| RsaI | GTAC | 5 cut(s) | 279, 604, 1137, 2111, 3002 |
| RsaNI | GTAC | 5 cut(s) | 278, 603, 1136, 2110, 3001 |
| RseI | CAYNNNNRTG | 5 cut(s) | 251, 324, 620, 1569, 1679 |
| SacI | GAGCTC | 1 cut(s) | 3284 |
| SapI | GCTCTTC | 1 cut(s) | 1261 |
| Sau3AI | GATC | 8 cut(s) | 694, 737, 1045, 1313, 1612, 1714, 2098, 2386 |
| Sau96I | GGNCC | 4 cut(s) | 128, 143, 919, 2063 |
| SchI | GAGTC | 6 cut(s) | 522, 1191, 1729, 2729, 2741, 2816 |
| ScrFI | CCNGG | 9 cut(s) | 126, 924, 1830, 2403, 2976, 3244, 3362, 3554, 3578 |
| SduI | GDGCHC | 4 cut(s) | 730, 2002, 3284, 3456 |
| SfcI | CTRYAG | 4 cut(s) | 3039, 3207, 3338, 3611 |
| Sfr274I | CTCGAG | 1 cut(s) | 1993 |
| SinI | GGWCC | 1 cut(s) | 143 |
| SlaI | CTCGAG | 1 cut(s) | 1993 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 251, 324, 620, 1569, 1679 |
| SmlI | CTYRAG | 5 cut(s) | 428, 550, 1993, 2087, 3725 |
| SmoI | CTYRAG | 5 cut(s) | 428, 550, 1993, 2087, 3725 |
| SpeI | ACTAGT | 1 cut(s) | 800 |
| SphI | GCATGC | 2 cut(s) | 1651, 2279 |
| SspI | AATATT | 4 cut(s) | 2143, 3380, 3429, 3543 |
| SspMI | CTAG | 8 cut(s) | 749, 801, 914, 1251, 1901, 2601, 2951, 3371 |
| SstI | GAGCTC | 1 cut(s) | 3284 |
| StyD4I | CCNGG | 9 cut(s) | 124, 922, 1828, 2401, 2974, 3242, 3360, 3552, 3576 |
| StyI | CCWWGG | 5 cut(s) | 30, 162, 1379, 1782, 3443 |
| TaaI | ACNGT | 4 cut(s) | 1019, 1606, 2484, 3234 |
| TaiI | ACGT | 3 cut(s) | 208, 2071, 2634 |
| TaqI | TCGA | 5 cut(s) | 86, 266, 1243, 1994, 2323 |
| TatI | WGTACW | 2 cut(s) | 1135, 3000 |
| TauI | GCSGC | 4 cut(s) | 41, 377, 3442, 3576 |
| TscAI | CASTG | 9 cut(s) | 220, 1024, 1292, 1436, 1927, 1942, 2908, 3528, 3568 |
| TseFI | GTSAC | 1 cut(s) | 2636 |
| TseI | GCWGC | 9 cut(s) | 41, 371, 1073, 1985, 2023, 2302, 2476, 3209, 3337 |
| Tsp45I | GTSAC | 1 cut(s) | 2636 |
| TspDTI | ATGAA | 9 cut(s) | 276, 689, 920, 1701, 2460, 2861, 2982, 3614, 3827 |
| TspGWI | ACGGA | 3 cut(s) | 841, 1745, 2561 |
| TspRI | CASTG | 9 cut(s) | 220, 1024, 1292, 1436, 1927, 1942, 2908, 3528, 3568 |
| Tth111I | GACNNNGTC | 1 cut(s) | 3736 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 3291 |
| Vha464I | CTTAAG | 1 cut(s) | 3725 |
| VpaK11BI | GGWCC | 1 cut(s) | 143 |
| VspI | ATTAAT | 1 cut(s) | 3807 |
| XbaI | TCTAGA | 1 cut(s) | 913 |
| XceI | RCATGY | 3 cut(s) | 1651, 1678, 2279 |
| XhoI | CTCGAG | 1 cut(s) | 1993 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1537, 2378 |
| XspI | CTAG | 8 cut(s) | 749, 801, 914, 1251, 1901, 2601, 2951, 3371 |
| Zsp2I | ATGCAT | 1 cut(s) | 2281 |