DNA polymerase
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G64420 |
| fragaria_vesca | FvH4_2g11160 |
| malus_domestica | MD05G1049800.v1.1 MD10G1056900.v1.1 |
| prunus_persica | Prupe.8G075100_v2.0.a1 |
| pyrus_communis | pycom05g04160 pycom05g04170 pycom10g04380 |
| rosa_chinensis | RchiOBHm_Chr6g0269481 |
| rosa_laevigata | RLG00000013848 RLG00000013849 |
| rosa_multiflora | Rmu_ssc0000291.1_g000033 |
| rosa_roxburghii | Rroxscaffold_7G00198700 |
| rosa_rugosa | Rorug06G0048500 |
| rosa_samantha | Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100 |
| rosa_wichuraiana | Rw6G014500 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2482 |
| Acc36I | ACCTGC | 3 cut(s) | 2257, 2356, 2730 |
| AccII | CGCG | 2 cut(s) | 2778, 2780 |
| AccIII | TCCGGA | 2 cut(s) | 283, 2601 |
| AclWI | GGATC | 4 cut(s) | 678, 1056, 1069, 2633 |
| AcoI | YGGCCR | 2 cut(s) | 1721, 3334 |
| AcuI | CTGAAG | 7 cut(s) | 81, 963, 1360, 1443, 1704, 3080, 3459 |
| AcvI | CACGTG | 1 cut(s) | 1814 |
| AcyI | GRCGYC | 1 cut(s) | 160 |
| AdeI | CACNNNGTG | 1 cut(s) | 1881 |
| AfaI | GTAC | 3 cut(s) | 580, 1194, 3444 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 2878, 3209 |
| AflII | CTTAAG | 1 cut(s) | 3485 |
| AflIII | ACRYGT | 1 cut(s) | 1422 |
| AhlI | ACTAGT | 1 cut(s) | 776 |
| AjnI | CCWGG | 3 cut(s) | 3002, 3120, 3336 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 398, 430, 3187, 3219 |
| AleI | CACNNNNGTG | 1 cut(s) | 1685 |
| Alw21I | GWGCWC | 2 cut(s) | 274, 706 |
| Alw26I | GTCTC | 3 cut(s) | 89, 151, 2438 |
| AlwI | GGATC | 4 cut(s) | 678, 1056, 1069, 2633 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1780, 3092 |
| Ama87I | CYCGRG | 2 cut(s) | 1832, 3313 |
| Aor13HI | TCCGGA | 2 cut(s) | 283, 2601 |
| AoxI | GGCC | 8 cut(s) | 444, 896, 1098, 1721, 2490, 2997, 3200, 3334 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 658, 690, 3367, 3399 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 55, 1285, 1424, 2078, 2403 |
| AspLEI | GCGC | 3 cut(s) | 178, 478, 2780 |
| AspS9I | GGNCC | 2 cut(s) | 1808, 2869 |
| AsuC2I | CCSGG | 2 cut(s) | 3314, 3315 |
| AvaI | CYCGRG | 2 cut(s) | 1832, 3313 |
| AvaII | GGWCC | 2 cut(s) | 1808, 2869 |
| AxyI | CCTNAGG | 1 cut(s) | 2877 |
| BalI | TGGCCA | 2 cut(s) | 1723, 3336 |
| BamHI | GGATCC | 1 cut(s) | 1061 |
| BanII | GRGCYC | 1 cut(s) | 274 |
| BbrPI | CACGTG | 1 cut(s) | 1814 |
| BbsI | GAAGAC | 6 cut(s) | 249, 727, 773, 1515, 2166, 2382 |
| Bbv12I | GWGCWC | 2 cut(s) | 274, 706 |
| BbvCI | CCTCAGC | 1 cut(s) | 729 |
| BceAI | ACGGC | 1 cut(s) | 463 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 653, 687 |
| BciT130I | CCWGG | 3 cut(s) | 3004, 3122, 3338 |
| BciVI | GTATCC | 3 cut(s) | 1912, 2203, 2602 |
| BclI | TGATCA | 1 cut(s) | 3556 |
| BcnI | CCSGG | 2 cut(s) | 3314, 3315 |
| BcoDI | GTCTC | 3 cut(s) | 89, 151, 2438 |
| BcuI | ACTAGT | 1 cut(s) | 776 |
| BfmI | CTRYAG | 5 cut(s) | 171, 1304, 2187, 2799, 3098 |
| BfoI | RGCGCY | 1 cut(s) | 179 |
| BfrI | CTTAAG | 1 cut(s) | 3485 |
| BfuAI | ACCTGC | 3 cut(s) | 2257, 2356, 2730 |
| BfuI | GTATCC | 3 cut(s) | 1912, 2203, 2602 |
| BglII | AGATCT | 1 cut(s) | 3462 |
| BlpI | GCTNAGC | 2 cut(s) | 1997, 3491 |
| Bme1390I | CCNGG | 5 cut(s) | 3004, 3122, 3314, 3315, 3338 |
| Bme18I | GGWCC | 2 cut(s) | 1808, 2869 |
| BmeT110I | CYCGRG | 2 cut(s) | 1832, 3313 |
| BmgT120I | GGNCC | 2 cut(s) | 1808, 2869 |
| BmiI | GGNNCC | 2 cut(s) | 1063, 3008 |
| BmrFI | CCNGG | 5 cut(s) | 3004, 3122, 3314, 3315, 3338 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1834 |
| BpiI | GAAGAC | 6 cut(s) | 249, 727, 773, 1515, 2166, 2382 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 478, 510, 2480, 2512, 2492, 2524 |
| BpmI | CTGGAG | 2 cut(s) | 1757, 3504 |
| Bpu10I | CCTNAGC | 1 cut(s) | 729 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1997, 3491 |
| BpuEI | CTTGAG | 4 cut(s) | 422, 1202, 1723, 3491 |
| BpuMI | CCSGG | 2 cut(s) | 3314, 3315 |
| BsaAI | YACGTR | 1 cut(s) | 1814 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2613 |
| BsaHI | GRCGYC | 1 cut(s) | 160 |
| BsaI | GGTCTC | 1 cut(s) | 89 |
| BsaJI | CCNNGG | 8 cut(s) | 117, 195, 1127, 1923, 2541, 3203, 3312, 3313 |
| BsaWI | WCCGGW | 2 cut(s) | 283, 2601 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 2878, 3209 |
| Bse118I | RCCGGY | 1 cut(s) | 242 |
| Bse1I | ACTGG | 3 cut(s) | 1033, 1774, 2452 |
| Bse21I | CCTNAGG | 1 cut(s) | 2877 |
| Bse3DI | GCAATG | 7 cut(s) | 605, 786, 1324, 1533, 2213, 2222, 2601 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2613 |
| BseAI | TCCGGA | 2 cut(s) | 283, 2601 |
| BseBI | CCWGG | 3 cut(s) | 3004, 3122, 3338 |
| BseDI | CCNNGG | 8 cut(s) | 117, 195, 1127, 1923, 2541, 3203, 3312, 3313 |
| BseJI | GATNNNNATC | 1 cut(s) | 2613 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 2878, 3209 |
| BseMI | GCAATG | 7 cut(s) | 605, 786, 1324, 1533, 2213, 2222, 2601 |
| BseNI | ACTGG | 3 cut(s) | 1033, 1774, 2452 |
| BseRI | GAGGAG | 2 cut(s) | 746, 994 |
| BseYI | CCCAGC | 1 cut(s) | 2651 |
| BsgI | GTGCAG | 1 cut(s) | 401 |
| Bsh1236I | CGCG | 2 cut(s) | 2778, 2780 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2072 |
| BshFI | GGCC | 8 cut(s) | 446, 898, 1100, 1723, 2492, 2999, 3202, 3336 |
| BsiEI | CGRYCG | 1 cut(s) | 2072 |
| BsiHKAI | GWGCWC | 2 cut(s) | 274, 706 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1832, 3313 |
| BsiSI | CCGG | 4 cut(s) | 243, 284, 2602, 3314 |
| BslFI | GGGAC | 2 cut(s) | 3142, 3295 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 2878, 3209 |
| BsmAI | GTCTC | 3 cut(s) | 89, 151, 2438 |
| BsmBI | CGTCTC | 1 cut(s) | 151 |
| BsmFI | GGGAC | 2 cut(s) | 3142, 3295 |
| BsmI | GAATGC | 2 cut(s) | 22, 882 |
| BsnI | GGCC | 8 cut(s) | 446, 898, 1100, 1723, 2492, 2999, 3202, 3336 |
| Bso31I | GGTCTC | 1 cut(s) | 89 |
| BsoBI | CYCGRG | 2 cut(s) | 1832, 3313 |
| Bsp1286I | GDGCHC | 2 cut(s) | 274, 706 |
| Bsp13I | TCCGGA | 2 cut(s) | 283, 2601 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1997, 3491 |
| Bsp19I | CCATGG | 2 cut(s) | 117, 2541 |
| BspANI | GGCC | 8 cut(s) | 446, 898, 1100, 1723, 2492, 2999, 3202, 3336 |
| BspEI | TCCGGA | 2 cut(s) | 283, 2601 |
| BspFNI | CGCG | 2 cut(s) | 2778, 2780 |
| BspLI | GGNNCC | 2 cut(s) | 1063, 3008 |
| BspMAI | CTGCAG | 4 cut(s) | 175, 2191, 2803, 3102 |
| BspMI | ACCTGC | 3 cut(s) | 2257, 2356, 2730 |
| BspPI | GGATC | 4 cut(s) | 678, 1056, 1069, 2633 |
| BspQI | GCTCTTC | 1 cut(s) | 279 |
| BspTI | CTTAAG | 1 cut(s) | 3485 |
| BspTNI | GGTCTC | 1 cut(s) | 89 |
| BsrDI | GCAATG | 7 cut(s) | 605, 786, 1324, 1533, 2213, 2222, 2601 |
| BsrFI | RCCGGY | 1 cut(s) | 242 |
| BsrI | ACTGG | 3 cut(s) | 1033, 1774, 2452 |
| BssAI | RCCGGY | 1 cut(s) | 242 |
| BssECI | CCNNGG | 8 cut(s) | 117, 195, 1127, 1923, 2541, 3203, 3312, 3313 |
| BssNI | GRCGYC | 1 cut(s) | 160 |
| BssT1I | CCWWGG | 3 cut(s) | 117, 2541, 3203 |
| Bst2UI | CCWGG | 3 cut(s) | 3004, 3122, 3338 |
| Bst4CI | ACNGT | 7 cut(s) | 109, 192, 473, 1354, 1782, 2235, 2465 |
| Bst6I | CTCTTC | 2 cut(s) | 279, 800 |
| BstACI | GRCGYC | 1 cut(s) | 160 |
| BstAFI | CTTAAG | 1 cut(s) | 3485 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1881, 2680 |
| BstBAI | YACGTR | 1 cut(s) | 1814 |
| BstC8I | GCNNGC | 9 cut(s) | 234, 244, 825, 939, 1137, 1397, 1953, 2022, 2776 |
| BstDSI | CCRYGG | 3 cut(s) | 117, 195, 2541 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3207 |
| BstFNI | CGCG | 2 cut(s) | 2778, 2780 |
| BstH2I | RGCGCY | 1 cut(s) | 179 |
| BstHHI | GCGC | 3 cut(s) | 178, 478, 2780 |
| BstMAI | GTCTC | 3 cut(s) | 89, 151, 2438 |
| BstMCI | CGRYCG | 1 cut(s) | 2072 |
| BstNI | CCWGG | 3 cut(s) | 3004, 3122, 3338 |
| BstNSI | RCATGY | 3 cut(s) | 1399, 1426, 2024 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1834 |
| BstSCI | CCNGG | 5 cut(s) | 3002, 3120, 3312, 3313, 3336 |
| BstSFI | CTRYAG | 5 cut(s) | 171, 1304, 2187, 2799, 3098 |
| BstUI | CGCG | 2 cut(s) | 2778, 2780 |
| BstV2I | GAAGAC | 6 cut(s) | 249, 727, 773, 1515, 2166, 2382 |
| BstX2I | RGATCY | 3 cut(s) | 1061, 2625, 3462 |
| BstYI | RGATCY | 3 cut(s) | 1061, 2625, 3462 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2877 |
| BsuI | GTATCC | 3 cut(s) | 1912, 2203, 2602 |
| BsuRI | GGCC | 8 cut(s) | 446, 898, 1100, 1723, 2492, 2999, 3202, 3336 |
| BtgI | CCRYGG | 3 cut(s) | 117, 195, 2541 |
| BtgZI | GCGATG | 2 cut(s) | 1062, 1970 |
| BtsI | GCAGTG | 2 cut(s) | 1665, 2661 |
| BtsIMutI | CAGTG | 7 cut(s) | 950, 1040, 1184, 1665, 1680, 2470, 2661 |
| BveI | ACCTGC | 3 cut(s) | 2257, 2356, 2730 |
| Cac8I | GCNNGC | 9 cut(s) | 234, 244, 825, 939, 1137, 1397, 1953, 2022, 2776 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1780, 3092 |
| CfoI | GCGC | 3 cut(s) | 178, 478, 2780 |
| Cfr10I | RCCGGY | 1 cut(s) | 242 |
| Cfr13I | GGNCC | 2 cut(s) | 1808, 2869 |
| Cfr9I | CCCGGG | 1 cut(s) | 3313 |
| CseI | GACGC | 5 cut(s) | 98, 168, 243, 2189, 2564 |
| Csp6I | GTAC | 3 cut(s) | 579, 1193, 3443 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1800, 1835, 2733, 2768 |
| CviQI | GTAC | 3 cut(s) | 579, 1193, 3443 |
| DraI | TTTAAA | 3 cut(s) | 1593, 2275, 2860 |
| DraIII | CACNNNGTG | 1 cut(s) | 1881 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2482 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2482 |
| EaeI | YGGCCR | 2 cut(s) | 1721, 3334 |
| Eam1104I | CTCTTC | 2 cut(s) | 279, 800 |
| EarI | CTCTTC | 2 cut(s) | 279, 800 |
| EciI | GGCGGA | 2 cut(s) | 261, 282 |
| Ecl136II | GAGCTC | 1 cut(s) | 272 |
| Eco130I | CCWWGG | 3 cut(s) | 117, 2541, 3203 |
| Eco147I | AGGCCT | 1 cut(s) | 3202 |
| Eco24I | GRGCYC | 1 cut(s) | 274 |
| Eco31I | GGTCTC | 1 cut(s) | 89 |
| Eco47I | GGWCC | 2 cut(s) | 1808, 2869 |
| Eco53kI | GAGCTC | 1 cut(s) | 272 |
| Eco57I | CTGAAG | 7 cut(s) | 81, 963, 1360, 1443, 1704, 3080, 3459 |
| Eco72I | CACGTG | 1 cut(s) | 1814 |
| Eco81I | CCTNAGG | 1 cut(s) | 2877 |
| Eco88I | CYCGRG | 2 cut(s) | 1832, 3313 |
| EcoICRI | GAGCTC | 1 cut(s) | 272 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3207 |
| EcoRI | GAATTC | 3 cut(s) | 770, 2078, 2838 |
| EcoRII | CCWGG | 3 cut(s) | 3002, 3120, 3336 |
| EcoT14I | CCWWGG | 3 cut(s) | 117, 2541, 3203 |
| EcoT38I | GRGCYC | 1 cut(s) | 274 |
| ErhI | CCWWGG | 3 cut(s) | 117, 2541, 3203 |
| Esp3I | CGTCTC | 1 cut(s) | 151 |
| FaqI | GGGAC | 2 cut(s) | 3142, 3295 |
| FauI | CCCGC | 3 cut(s) | 241, 419, 3449 |
| FbaI | TGATCA | 1 cut(s) | 3556 |
| FriOI | GRGCYC | 1 cut(s) | 274 |
| GlaI | GCGC | 3 cut(s) | 177, 477, 2779 |
| GsaI | CCCAGC | 1 cut(s) | 2655 |
| GsuI | CTGGAG | 2 cut(s) | 1757, 3504 |
| HaeII | RGCGCY | 1 cut(s) | 179 |
| HaeIII | GGCC | 8 cut(s) | 446, 898, 1100, 1723, 2492, 2999, 3202, 3336 |
| HapII | CCGG | 4 cut(s) | 243, 284, 2602, 3314 |
| HgaI | GACGC | 5 cut(s) | 98, 168, 243, 2189, 2564 |
| HhaI | GCGC | 3 cut(s) | 178, 478, 2780 |
| Hin1I | GRCGYC | 1 cut(s) | 160 |
| Hin6I | GCGC | 3 cut(s) | 176, 476, 2778 |
| HinP1I | GCGC | 3 cut(s) | 176, 476, 2778 |
| HincII | GTYRAC | 1 cut(s) | 2290 |
| HindII | GTYRAC | 1 cut(s) | 2290 |
| HindIII | AAGCTT | 3 cut(s) | 54, 2690, 3342 |
| HpaII | CCGG | 4 cut(s) | 243, 284, 2602, 3314 |
| Hpy166II | GTNNAC | 7 cut(s) | 195, 601, 1665, 1811, 2290, 2761, 2813 |
| Hpy8I | GTNNAC | 7 cut(s) | 195, 601, 1665, 1811, 2290, 2761, 2813 |
| Hpy99I | CGWCG | 2 cut(s) | 367, 484 |
| HpyCH4III | ACNGT | 7 cut(s) | 109, 192, 473, 1354, 1782, 2235, 2465 |
| HpyCH4IV | ACGT | 1 cut(s) | 1813 |
| HpySE526I | ACGT | 1 cut(s) | 1813 |
| Hsp92I | GRCGYC | 1 cut(s) | 160 |
| HspAI | GCGC | 3 cut(s) | 176, 476, 2778 |
| Kpn2I | TCCGGA | 2 cut(s) | 283, 2601 |
| KroI | GCCGGC | 1 cut(s) | 242 |
| KroNI | GCCGGC | 1 cut(s) | 244 |
| Ksp22I | TGATCA | 1 cut(s) | 3556 |
| LguI | GCTCTTC | 1 cut(s) | 279 |
| LmnI | GCTCC | 9 cut(s) | 248, 269, 367, 661, 1007, 1776, 2067, 2188, 2248 |
| MaeII | ACGT | 1 cut(s) | 1813 |
| MaeIII | GTNAC | 5 cut(s) | 637, 1687, 1776, 2459, 2657 |
| MfeI | CAATTG | 1 cut(s) | 3398 |
| MflI | RGATCY | 3 cut(s) | 1061, 2625, 3462 |
| MhlI | GDGCHC | 2 cut(s) | 274, 706 |
| MlsI | TGGCCA | 2 cut(s) | 1723, 3336 |
| MluNI | TGGCCA | 2 cut(s) | 1723, 3336 |
| MmeI | TCCRAC | 2 cut(s) | 420, 1223 |
| Mox20I | TGGCCA | 2 cut(s) | 1723, 3336 |
| MroI | TCCGGA | 2 cut(s) | 283, 2601 |
| MroNI | GCCGGC | 1 cut(s) | 242 |
| MroXI | GAANNNNTTC | 5 cut(s) | 55, 1285, 1424, 2078, 2403 |
| MscI | TGGCCA | 2 cut(s) | 1723, 3336 |
| MslI | CAYNNNNRTG | 5 cut(s) | 596, 1317, 1427, 1685, 2361 |
| Msp20I | TGGCCA | 2 cut(s) | 1723, 3336 |
| MspA1I | CMGCKG | 2 cut(s) | 239, 2047 |
| MspCI | CTTAAG | 1 cut(s) | 3485 |
| MspI | CCGG | 4 cut(s) | 243, 284, 2602, 3314 |
| MspR9I | CCNGG | 5 cut(s) | 3004, 3122, 3314, 3315, 3338 |
| MunI | CAATTG | 1 cut(s) | 3398 |
| Mva1269I | GAATGC | 2 cut(s) | 22, 882 |
| MvaI | CCWGG | 3 cut(s) | 3004, 3122, 3338 |
| MvnI | CGCG | 2 cut(s) | 2778, 2780 |
| NaeI | GCCGGC | 1 cut(s) | 244 |
| NciI | CCSGG | 2 cut(s) | 3314, 3315 |
| NcoI | CCATGG | 2 cut(s) | 117, 2541 |
| NgoMIV | GCCGGC | 1 cut(s) | 242 |
| NlaIV | GGNNCC | 2 cut(s) | 1063, 3008 |
| NmeAIII | GCCGAG | 1 cut(s) | 472 |
| NmuCI | GTSAC | 4 cut(s) | 637, 1687, 2459, 2657 |
| NspI | RCATGY | 3 cut(s) | 1399, 1426, 2024 |
| OliI | CACNNNNGTG | 1 cut(s) | 1685 |
| PaeI | GCATGC | 2 cut(s) | 1399, 2024 |
| PaeR7I | CTCGAG | 1 cut(s) | 1832 |
| PceI | AGGCCT | 1 cut(s) | 3202 |
| PciI | ACATGT | 1 cut(s) | 1422 |
| PciSI | GCTCTTC | 1 cut(s) | 279 |
| PctI | GAATGC | 2 cut(s) | 22, 882 |
| PdiI | GCCGGC | 1 cut(s) | 244 |
| PdmI | GAANNNNTTC | 5 cut(s) | 55, 1285, 1424, 2078, 2403 |
| PfoI | TCCNGGA | 1 cut(s) | 3002 |
| PmaCI | CACGTG | 1 cut(s) | 1814 |
| PmlI | CACGTG | 1 cut(s) | 1814 |
| Ppu21I | YACGTR | 1 cut(s) | 1814 |
| PscI | ACATGT | 1 cut(s) | 1422 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1834 |
| Psp124BI | GAGCTC | 1 cut(s) | 274 |
| Psp6I | CCWGG | 3 cut(s) | 3002, 3120, 3336 |
| PspCI | CACGTG | 1 cut(s) | 1814 |
| PspFI | CCCAGC | 1 cut(s) | 2651 |
| PspGI | CCWGG | 3 cut(s) | 3002, 3120, 3336 |
| PspN4I | GGNNCC | 2 cut(s) | 1063, 3008 |
| PspPI | GGNCC | 2 cut(s) | 1808, 2869 |
| PspXI | VCTCGAGB | 1 cut(s) | 1832 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2184, 2216 |
| PstI | CTGCAG | 4 cut(s) | 175, 2191, 2803, 3102 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1780, 3092 |
| PsuI | RGATCY | 3 cut(s) | 1061, 2625, 3462 |
| PvuII | CAGCTG | 1 cut(s) | 2047 |
| RsaI | GTAC | 3 cut(s) | 580, 1194, 3444 |
| RsaNI | GTAC | 3 cut(s) | 579, 1193, 3443 |
| RseI | CAYNNNNRTG | 5 cut(s) | 596, 1317, 1427, 1685, 2361 |
| SacI | GAGCTC | 1 cut(s) | 274 |
| SapI | GCTCTTC | 1 cut(s) | 279 |
| Sau96I | GGNCC | 2 cut(s) | 1808, 2869 |
| ScrFI | CCNGG | 5 cut(s) | 3004, 3122, 3314, 3315, 3338 |
| SduI | GDGCHC | 2 cut(s) | 274, 706 |
| SfcI | CTRYAG | 5 cut(s) | 171, 1304, 2187, 2799, 3098 |
| Sfr274I | CTCGAG | 1 cut(s) | 1832 |
| SinI | GGWCC | 2 cut(s) | 1808, 2869 |
| SlaI | CTCGAG | 1 cut(s) | 1832 |
| SmaI | CCCGGG | 1 cut(s) | 3315 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 596, 1317, 1427, 1685, 2361 |
| SmlI | CTYRAG | 6 cut(s) | 401, 1217, 1738, 1832, 3485, 3506 |
| SmoI | CTYRAG | 6 cut(s) | 401, 1217, 1738, 1832, 3485, 3506 |
| SpeI | ACTAGT | 1 cut(s) | 776 |
| SphI | GCATGC | 2 cut(s) | 1399, 2024 |
| SseBI | AGGCCT | 1 cut(s) | 3202 |
| SspI | AATATT | 5 cut(s) | 913, 2856, 3140, 3189, 3303 |
| SstI | GAGCTC | 1 cut(s) | 274 |
| StuI | AGGCCT | 1 cut(s) | 3202 |
| StyD4I | CCNGG | 5 cut(s) | 3002, 3120, 3312, 3313, 3336 |
| StyI | CCWWGG | 3 cut(s) | 117, 2541, 3203 |
| TaaI | ACNGT | 7 cut(s) | 109, 192, 473, 1354, 1782, 2235, 2465 |
| TaiI | ACGT | 1 cut(s) | 1816 |
| TaqI | TCGA | 9 cut(s) | 14, 527, 848, 1828, 1833, 1909, 2068, 2695, 3285 |
| TaqII | GACCGA | 1 cut(s) | 2086 |
| TatI | WGTACW | 1 cut(s) | 1192 |
| TauI | GCSGC | 5 cut(s) | 232, 239, 267, 332, 347 |
| TscAI | CASTG | 7 cut(s) | 957, 1040, 1184, 1672, 1687, 2470, 2668 |
| TseFI | GTSAC | 4 cut(s) | 637, 1687, 2459, 2657 |
| Tsp45I | GTSAC | 4 cut(s) | 637, 1687, 2459, 2657 |
| TspGWI | ACGGA | 5 cut(s) | 212, 380, 2312, 2638, 3460 |
| TspMI | CCCGGG | 1 cut(s) | 3313 |
| TspRI | CASTG | 7 cut(s) | 957, 1040, 1184, 1672, 1687, 2470, 2668 |
| Vha464I | CTTAAG | 1 cut(s) | 3485 |
| VpaK11BI | GGWCC | 2 cut(s) | 1808, 2869 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3207 |
| XbaI | TCTAGA | 1 cut(s) | 2905 |
| XceI | RCATGY | 3 cut(s) | 1399, 1426, 2024 |
| XhoI | CTCGAG | 1 cut(s) | 1832 |
| XmaI | CCCGGG | 1 cut(s) | 3313 |
| XmnI | GAANNNNTTC | 5 cut(s) | 55, 1285, 1424, 2078, 2403 |