FvH4_2g11160

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010240
Physical Location & Seq
Reverse (-)
9732689 .. 9739427
6739 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11160.t1

Sequence Viewer

Length: 3765 bp
ATGGAGGTCCTGAAACACAAGAAAAGAAAAACCCCTGAGGAAACCAAACCCTCCAAACCAATGGAAGCCGAGGCCGCCGCCAAGCCCATGGAGAAGCAGAAGAAACGAAAAGCTATGGACAAAGAGAGACGCCTCCACGCCGCACTGGAAGCCAAGCCTAAACCACCTCCGTCCATCTCCGAATTTAAAACCGCCGCGGCTGCTTCTTCTAGCGGCGGGGCTGTTCTGCCGGAGTTCCACGTCGGCGTGTTCAAGGACTTGGCTTCTGCTGATGCCGCGGTGAGAGAGGCGGCGGTGGAGGCCTTGGCGACGGAGCTGATGGAAGTGCAGAGGGCTTATGAGGGGCTTGAGAATAAGGAGTTGTTGGAAGGCGGTGGTGTCAAGCTGGAGGCGGAGAAGGATGACGGCTTGAATGACTGTGCGCCGTCGTTGAGATATGCTCTGCGGAGACTCATTCGTGGTGTTTCTTCATCTAGAGAGTGTGCAAGACAAGGATTTGCAGTAGGTTTAACTATGTTAGGAAGTACCATTCGCAGCATCAAGGTGGACTCGCTGCTGAAACTCATAGTTGATTTCTTAGAGGTCACTTCATCAATGAAGGGTCAGGAACAAAGGGATCGTCTTTTGGGTCGATTATTTGCGTATGGTGCTCTTGGACGATCAGGAAGACTAGTTGAGGAGTGGGTTTCTGATAGAAATACTCCGCACATTAAGGAATTCACTAGTCTCCTCATTGCTCTTGCATCAAAGAAGAGATACTTGCAAGAGCCTGCTGTATCAGTTATTTTAGACTTGATTGAAAAGTTGCCCCCAGAAGCCTTGTTGATTCATGTTCTAGAGGCTCCAGGACTTCATGAATGGTTTGAAGGAGCTATTGAAATTGGAAATCCTGATGCTTTGCTGTTGGCTCTGAAAATTGGAGAAAAAGTTTCTGTGGACAGTGCAAGATTTGGGAAACTTCTGCCAGACCCATTTGTTCCTAACAAATTGTTCTCTGCTGAGCACCTGTCTTCTCTTGCTAATAGTTTAAAGGAATCGACCTTCTGTCAGCCACGCATTCACAGTGTTTGGCCTGTTCTAGTGAATATTCTTTTGCCTGAAAGAGTTTTACAGACTGAAGATGCAGTGTCTATTTCAAATTCCTTAAAGAAGCACAAAAAGAATCGCAAATCTAGCTCTTCTGATGAAGATATTGCAAAGAATTTCCAGTGCTTCTGCGAGGTGATTATTGAGGGATCCCTTCTCCCATCATCTCATGATCGCAAACACTTGGCCTTTGATGTTTTGCTTCTTCTCCTACCGAGGTTGCCTGCTTCTTATATACCAATTTGTTTGTCTTACAAAGTTGTTCAGTGCATGACTGATGTACTTCCAACAACTGATGCTTGGCTCAAGAAAATTGTGCAAAACTTTATTAAGACATTATCAGACTGGGTTGGGGATGATGATGTCAAAAGGGTTTCTGTAATAATGGCTCTACAGAAACACAGCAATGGAAGATTTGATTGTATCACGCGAACAAAAACAGTTAAGGATTTGATGGCAGACTTCAAAACAGAATCGGGTTGCATGCTGTTTATTCAGAACTTGTTGAACATGTTTGTGGATGAAAGTCATGCTTCTGATGAACCTTCGGATCAGAGTATAACAACAGATGACAATTCTGAGATAGGCTCTATTGAAGACAAGGACTCTGTTGCAATGGGAAATTCTGATATTTTGAAAGCTTGGATAGTGGAATCCCTCCCCTGTATATTAAAAAATTTAAAGCTAGAACCAGAAGCTAAGTTTAGGGTTCAAAAAGAAATTTTGAAGTTTCTAGCTGTTCAGGGTTTGTTCACTGCATCTCTTGGCACTGAAGTGACATCCTTTGAATTACAAGAGAAATTTAGGTGGCCAAAAGTAGCCACCTCAAGTGCCCTCTGCAGGATGTGTATTGAGCAGCTCCAGTTATTATTGGCAAATTCCCAGAAGGGAGAAGGTCCACGTGGTTTGCCAAATCGACTCGAATCAAATGATCTTGGCTCATACTTTATGCGGTTTCTTAGCACTTTGTGCAACATTCCTTCAATCTCTCTCTTTCGACCTTTGGATACCGAGGAAGAAAATACGTTGAAAAAATTGCAAGCTATGGAGACTTCACTTTCTAAAGAGGAAAGGAATTGCGGGCATAGCAGCGAGGCAAATAGGTTGCATGCGCTAAGATACTTGCTCATCCAGCTGCTTTTGCAAATGCTCCTTCGACCTAAAGAGTTCTTAGTAGCAGTGTCTGAGCTTATTATATGCTGTAAAAAGGCTTTCCCTGTTGTTGATGTCGTTGATTCCGGTGAAGACAACTTAGATGGTGATGATGCACCTGCAGTGATGGATGTTCTTGTGGATACACTGCTCTCATTGCTGCCTCAGTCATCAGCTCCTATGCGAACTGCTATTGAACAGGTTTTTAAATACTTCTGTGTTGACATCACGGATGATGGTCTACTGCGGATGTTGAGGGTCATTAGGAAGAACTTGAAACCTGTCAGACATCAGGATGCTGATAGTGAAGACATTGACGATGACGAAGATGAAGATTTTCTTAACATTGAAGAAGATGAGGTCATTGATAGGGCTGAGACCGGTGAAACAGGTGACAGTGAGCAGACAGACGAGTCTGAGGCCGACTCTGAGGCTGACTCTGAGGCTGTTGATGAGGTTGAGGAAGTTGCCCAGGAAATTCATGACGCTTCTGATGAATCAGATGGAGGGATGGATGATGATGCAATGTTCCGGATGGATACTTATCTTGCACGGATCTTCAAAGAGCGAAGGAATCTGGCTGGGGGTGACACTGCTCATCAGCAACTCATGCTGTTTAAGCTTCGTGTTCTTTCTTTGCTAGAAATTTACCTGCATGAAAATCCAGATAAGCCACAAGTTTTGTTGGTTTACTCAAATTTGGCTCGTGCGTTTGCTGAACCGCATACTGCAGAGAGTAGTGAACAGCTTGGACAGCGTATATGGGGAATTCTGCAAAAGAAAATATTTAAAGCGAAGGACCATCCCAAGGGTGAAGATGTGCAACTATCTACTCTAGAATCTTTGTTGCAAAGAAATCTGAAGTTGGCTTCAAAACCAATCAAGAGAAAGAAATCTGCTGCCAACCTGTCAAAGAAGAAGCAGTCGGCTTCCTGGAACCGACAGAAAATTATTGCTTCCCTTGCACAGAGTTCTACCTTCTGGATTCTAAAGATCATTGATGCAAGAAATTTTCCAGAGTCTGAGCTGCAGAGAGTTTTTGATATATTCCAGGGTGTTCTGGTAGAGTATTTCAATAGTAAGAAGTCTCAAATCAAATCGGAGTTTCTGAAAGAAATATTTAGAAGAAGGCCTTGGATAGGGCGTTATCTCTTTGGCTTTCTTTTGGAGAAATGTGGAAGTTCAAAGTCAGACTTTCGGCGAGTAGAAGCACTTGATATGGTGAGTGAAATATTGAAGTCCCCAGGCTTATCTGATGTAAGCGGCGAGGAAACTCTGAAGAAAATTATGAAAAGCCATCTTGAAAAACTCTGTCAGTTGATAGAGCAATTGCTTACAAATATGCCAGAGAAACAGTCAAGACGAGCTGAAGTACGGAAGTTTTGTGGGAAGATCTTTCAGATGATAGCAACCCTTAAGCTGAGCAAGTCTTTTCTTAAGAATTTAGCTCCAGATGCTCACGCCAAGTGTGAATCACAACTTGGTGATCAGTTTAAGAACTTGAAGAAGATACAGCTGAAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1255

Amino Acids

140.92

Weight (kDa)

5.88

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 146 - 964 5.2e-179 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2365
AasI GACNNNNNNGTC 1 cut(s) 2650
Acc36I ACCTGC 2 cut(s) 2365, 2898
AccI GTMKAC 1 cut(s) 2479
AccII CGCG 3 cut(s) 197, 278, 1516
AccIII TCCGGA 1 cut(s) 2769
AclWI GGATC 5 cut(s) 624, 1230, 1243, 1644, 2801
AcoI YGGCCR 1 cut(s) 1895
AcuI CTGAAG 5 cut(s) 1137, 1878, 3119, 3536, 3627
AcvI CACGTG 1 cut(s) 1988
AcyI GRCGYC 1 cut(s) 130
AdeI CACNNNGTG 1 cut(s) 2055
AfaI GTAC 3 cut(s) 526, 1368, 3612
AfiI CCNNNNNNNGG 3 cut(s) 1926, 3046, 3377
AflII CTTAAG 2 cut(s) 3653, 3674
AflIII ACRYGT 1 cut(s) 1596
AgeI ACCGGT 1 cut(s) 2618
AhdI GACNNNNNGTC 1 cut(s) 1044
AhlI ACTAGT 2 cut(s) 670, 722
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 5 cut(s) 844, 2709, 3170, 3288, 3481
AjuI GAANNNNNNNTTGG 4 cut(s) 3355, 3387, 3702, 3734
AleI CACNNNNGTG 1 cut(s) 1859
Alw21I GWGCWC 2 cut(s) 652, 1005
Alw26I GTCTC 6 cut(s) 121, 442, 731, 2129, 2609, 3330
AlwI GGATC 5 cut(s) 624, 1230, 1243, 1644, 2801
AlwNI CAGNNNCTG 2 cut(s) 2270, 3260
Aor13HI TCCGGA 1 cut(s) 2769
AoxI GGCC 7 cut(s) 72, 300, 1070, 1272, 1895, 2658, 3368
ApeKI GCWGC 9 cut(s) 200, 534, 553, 1942, 2175, 2221, 2398, 3137, 3265
ArsI GACNNNNNNTTYG 2 cut(s) 604, 636
AsiGI ACCGGT 1 cut(s) 2618
Asp700I GAANNNNTTC 1 cut(s) 2574
AspLEI GCGC 2 cut(s) 424, 2200
AspS9I GGNCC 3 cut(s) 7, 1982, 3037
AvaII GGWCC 3 cut(s) 7, 1982, 3037
AxyI CCTNAGG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 1921
BalI TGGCCA 1 cut(s) 1897
BamHI GGATCC 1 cut(s) 1235
BauI CACGAG 1 cut(s) 2943
BbrPI CACGTG 1 cut(s) 1988
BbsI GAAGAC 5 cut(s) 673, 1002, 1689, 2337, 2553
Bbv12I GWGCWC 2 cut(s) 652, 1005
BbvI GCAGC 9 cut(s) 187, 540, 546, 1954, 2187, 2208, 2385, 3124, 3252
BceAI ACGGC 2 cut(s) 409, 421
BciT130I CCWGG 5 cut(s) 846, 2711, 3172, 3290, 3483
BciVI GTATCC 3 cut(s) 2086, 2374, 2770
BclI TGATCA 1 cut(s) 3724
BcoDI GTCTC 6 cut(s) 121, 442, 731, 2129, 2609, 3330
BcuI ACTAGT 2 cut(s) 670, 722
BfmI CTRYAG 5 cut(s) 1478, 1924, 2358, 2967, 3266
BfrI CTTAAG 2 cut(s) 3653, 3674
BfuAI ACCTGC 2 cut(s) 2365, 2898
BfuI GTATCC 3 cut(s) 2086, 2374, 2770
BglII AGATCT 1 cut(s) 3630
BlpI GCTNAGC 2 cut(s) 999, 3659
Bme1390I CCNGG 5 cut(s) 846, 2711, 3172, 3290, 3483
Bme18I GGWCC 3 cut(s) 7, 1982, 3037
BmeRI GACNNNNNGTC 1 cut(s) 1044
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 7, 1982, 3037
BmiI GGNNCC 3 cut(s) 843, 1237, 3176
BmrFI CCNGG 5 cut(s) 846, 2711, 3172, 3290, 3483
BmrI ACTGGG 1 cut(s) 1441
BmuI ACTGGG 1 cut(s) 1441
BpiI GAAGAC 5 cut(s) 673, 1002, 1689, 2337, 2553
BplI GAGNNNNNCTC 8 cut(s) 424, 456, 1658, 1690, 2648, 2680, 2660, 2692
BpmI CTGGAG 4 cut(s) 407, 828, 1931, 3672
Bpu1102I GCTNAGC 2 cut(s) 999, 3659
BpuEI CTTGAG 3 cut(s) 368, 1376, 1897
BsaAI YACGTR 1 cut(s) 1988
BsaBI GATNNNNATC 1 cut(s) 2781
BsaHI GRCGYC 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 2609
BsaWI WCCGGW 3 cut(s) 2324, 2618, 2769
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 3 cut(s) 1926, 3046, 3377
Bse118I RCCGGY 1 cut(s) 2618
Bse1I ACTGG 4 cut(s) 150, 1207, 1436, 1948
Bse21I CCTNAGG 1 cut(s) 36
Bse3DI GCAATG 5 cut(s) 732, 1498, 1707, 2393, 2769
Bse8I GATNNNNATC 1 cut(s) 2781
BseAI TCCGGA 1 cut(s) 2769
BseBI CCWGG 5 cut(s) 846, 2711, 3172, 3290, 3483
BseJI GATNNNNATC 1 cut(s) 2781
BseLI CCNNNNNNNGG 3 cut(s) 1926, 3046, 3377
BseMI GCAATG 5 cut(s) 732, 1498, 1707, 2393, 2769
BseNI ACTGG 4 cut(s) 150, 1207, 1436, 1948
BseRI GAGGAG 2 cut(s) 692, 719
BseSI GKGCMC 1 cut(s) 1921
BseXI GCAGC 9 cut(s) 187, 540, 546, 1954, 2187, 2208, 2385, 3124, 3252
BseYI CCCAGC 1 cut(s) 2819
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 3 cut(s) 197, 278, 1516
BshFI GGCC 7 cut(s) 74, 302, 1072, 1274, 1897, 2660, 3370
BshTI ACCGGT 1 cut(s) 2618
BsiHKAI GWGCWC 2 cut(s) 652, 1005
BsiSI CCGG 4 cut(s) 230, 2325, 2619, 2770
BslFI GGGAC 1 cut(s) 3463
BslI CCNNNNNNNGG 3 cut(s) 1926, 3046, 3377
BsmAI GTCTC 6 cut(s) 121, 442, 731, 2129, 2609, 3330
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 3463
BsmI GAATGC 1 cut(s) 1056
BsnI GGCC 7 cut(s) 74, 302, 1072, 1274, 1897, 2660, 3370
Bso31I GGTCTC 1 cut(s) 2609
Bsp1286I GDGCHC 3 cut(s) 652, 1005, 1921
Bsp13I TCCGGA 1 cut(s) 2769
Bsp1720I GCTNAGC 2 cut(s) 999, 3659
Bsp19I CCATGG 1 cut(s) 87
BspANI GGCC 7 cut(s) 74, 302, 1072, 1274, 1897, 2660, 3370
BspEI TCCGGA 1 cut(s) 2769
BspFNI CGCG 3 cut(s) 197, 278, 1516
BspHI TCATGA 3 cut(s) 853, 1255, 2719
BspLI GGNNCC 3 cut(s) 843, 1237, 3176
BspMAI CTGCAG 4 cut(s) 1928, 2362, 2971, 3270
BspMI ACCTGC 2 cut(s) 2365, 2898
BspPI GGATC 5 cut(s) 624, 1230, 1243, 1644, 2801
BspQI GCTCTTC 1 cut(s) 1183
BspTI CTTAAG 2 cut(s) 3653, 3674
BspTNI GGTCTC 1 cut(s) 2609
BsrDI GCAATG 5 cut(s) 732, 1498, 1707, 2393, 2769
BsrFI RCCGGY 1 cut(s) 2618
BsrI ACTGG 4 cut(s) 150, 1207, 1436, 1948
BssAI RCCGGY 1 cut(s) 2618
BssNI GRCGYC 1 cut(s) 130
BssSI CACGAG 1 cut(s) 2943
BssT1I CCWWGG 4 cut(s) 87, 303, 3045, 3371
Bst2BI CACGAG 1 cut(s) 2943
Bst2UI CCWGG 5 cut(s) 846, 2711, 3172, 3290, 3483
Bst4CI ACNGT 6 cut(s) 419, 941, 1064, 1528, 2636, 3594
Bst6I CTCTTC 2 cut(s) 746, 1183
BstACI GRCGYC 1 cut(s) 130
BstAFI CTTAAG 2 cut(s) 3653, 3674
BstAPI GCANNNNNTGC 2 cut(s) 2055, 2848
BstBAI YACGTR 1 cut(s) 1988
BstC8I GCNNGC 6 cut(s) 771, 1311, 1571, 2127, 2168, 2196
BstDSI CCRYGG 3 cut(s) 87, 195, 276
BstENI CCTNNNNNAGG 1 cut(s) 3375
BstFNI CGCG 3 cut(s) 197, 278, 1516
BstHHI GCGC 2 cut(s) 424, 2200
BstMAI GTCTC 6 cut(s) 121, 442, 731, 2129, 2609, 3330
BstNI CCWGG 5 cut(s) 846, 2711, 3172, 3290, 3483
BstNSI RCATGY 3 cut(s) 1573, 1600, 2198
BstSCI CCNGG 5 cut(s) 844, 2709, 3170, 3288, 3481
BstSFI CTRYAG 5 cut(s) 1478, 1924, 2358, 2967, 3266
BstSLI GKGCMC 1 cut(s) 1921
BstUI CGCG 3 cut(s) 197, 278, 1516
BstV1I GCAGC 9 cut(s) 187, 540, 546, 1954, 2187, 2208, 2385, 3124, 3252
BstV2I GAAGAC 5 cut(s) 673, 1002, 1689, 2337, 2553
BstX2I RGATCY 3 cut(s) 1235, 2793, 3630
BstXI CCANNNNNNTGG 2 cut(s) 61, 88
BstYI RGATCY 3 cut(s) 1235, 2793, 3630
Bsu36I CCTNAGG 1 cut(s) 36
BsuI GTATCC 3 cut(s) 2086, 2374, 2770
BsuRI GGCC 7 cut(s) 74, 302, 1072, 1274, 1897, 2660, 3370
BtgI CCRYGG 3 cut(s) 87, 195, 276
BtrI CACGTC 1 cut(s) 241
BtsI GCAGTG 6 cut(s) 1131, 1839, 2271, 2367, 2384, 2829
BveI ACCTGC 2 cut(s) 2365, 2898
Cac8I GCNNGC 6 cut(s) 771, 1311, 1571, 2127, 2168, 2196
CaiI CAGNNNCTG 2 cut(s) 2270, 3260
CciI TCATGA 3 cut(s) 853, 1255, 2719
CfoI GCGC 2 cut(s) 424, 2200
Cfr10I RCCGGY 1 cut(s) 2618
Cfr13I GGNCC 3 cut(s) 7, 1982, 3037
Cfr42I CCGCGG 2 cut(s) 198, 279
CseI GACGC 2 cut(s) 138, 2732
Csp6I GTAC 3 cut(s) 525, 1367, 3611
CspAI ACCGGT 1 cut(s) 2618
CspCI CAANNNNNGTGG 4 cut(s) 1974, 2009, 2901, 2936
CviQI GTAC 3 cut(s) 525, 1367, 3611
DraI TTTAAA 5 cut(s) 187, 1029, 1767, 2446, 3028
DraIII CACNNNGTG 1 cut(s) 2055
DrdI GACNNNNNNGTC 1 cut(s) 2650
DriI GACNNNNNGTC 1 cut(s) 1044
DseDI GACNNNNNNGTC 1 cut(s) 2650
EaeI YGGCCR 1 cut(s) 1895
Eam1104I CTCTTC 2 cut(s) 746, 1183
Eam1105I GACNNNNNGTC 1 cut(s) 1044
EarI CTCTTC 2 cut(s) 746, 1183
EciI GGCGGA 1 cut(s) 407
Eco130I CCWWGG 4 cut(s) 87, 303, 3045, 3371
Eco147I AGGCCT 2 cut(s) 302, 3370
Eco31I GGTCTC 1 cut(s) 2609
Eco47I GGWCC 3 cut(s) 7, 1982, 3037
Eco57I CTGAAG 5 cut(s) 1137, 1878, 3119, 3536, 3627
Eco72I CACGTG 1 cut(s) 1988
Eco81I CCTNAGG 1 cut(s) 36
EcoNI CCTNNNNNAGG 1 cut(s) 3375
EcoO109I RGGNCCY 1 cut(s) 7
EcoRI GAATTC 2 cut(s) 716, 3006
EcoRII CCWGG 5 cut(s) 844, 2709, 3170, 3288, 3481
EcoT14I CCWWGG 4 cut(s) 87, 303, 3045, 3371
ErhI CCWWGG 4 cut(s) 87, 303, 3045, 3371
Esp3I CGTCTC 1 cut(s) 121
FaqI GGGAC 1 cut(s) 3463
FauI CCCGC 2 cut(s) 209, 2159
FbaI TGATCA 1 cut(s) 3724
FblI GTMKAC 1 cut(s) 2479
GlaI GCGC 2 cut(s) 423, 2199
GsaI CCCAGC 1 cut(s) 2823
GsuI CTGGAG 4 cut(s) 407, 828, 1931, 3672
HaeIII GGCC 7 cut(s) 74, 302, 1072, 1274, 1897, 2660, 3370
HapII CCGG 4 cut(s) 230, 2325, 2619, 2770
HgaI GACGC 2 cut(s) 138, 2732
HhaI GCGC 2 cut(s) 424, 2200
Hin1I GRCGYC 1 cut(s) 130
Hin6I GCGC 2 cut(s) 422, 2198
HinP1I GCGC 2 cut(s) 422, 2198
HincII GTYRAC 1 cut(s) 2461
HindII GTYRAC 1 cut(s) 2461
HindIII AAGCTT 2 cut(s) 1725, 2858
HpaII CCGG 4 cut(s) 230, 2325, 2619, 2770
Hpy166II GTNNAC 8 cut(s) 547, 937, 1839, 1985, 2461, 2480, 2929, 2981
Hpy8I GTNNAC 8 cut(s) 547, 937, 1839, 1985, 2461, 2480, 2929, 2981
Hpy99I CGWCG 3 cut(s) 245, 313, 430
HpyCH4III ACNGT 6 cut(s) 419, 941, 1064, 1528, 2636, 3594
HpyCH4IV ACGT 3 cut(s) 240, 1987, 2111
HpySE526I ACGT 3 cut(s) 240, 1987, 2111
Hsp92I GRCGYC 1 cut(s) 130
HspAI GCGC 2 cut(s) 422, 2198
Kpn2I TCCGGA 1 cut(s) 2769
Ksp22I TGATCA 1 cut(s) 3724
KspI CCGCGG 2 cut(s) 198, 279
LguI GCTCTTC 1 cut(s) 1183
LmnI GCTCC 7 cut(s) 313, 847, 869, 1950, 2241, 2419, 3691
Lsp1109I GCAGC 9 cut(s) 187, 540, 546, 1954, 2187, 2208, 2385, 3124, 3252
MaeII ACGT 3 cut(s) 240, 1987, 2111
MaeIII GTNAC 4 cut(s) 583, 1861, 2630, 2825
MfeI CAATTG 1 cut(s) 3566
MflI RGATCY 3 cut(s) 1235, 2793, 3630
MhlI GDGCHC 3 cut(s) 652, 1005, 1921
MlsI TGGCCA 1 cut(s) 1897
MluNI TGGCCA 1 cut(s) 1897
MlyI GAGTC 8 cut(s) 444, 542, 1685, 1998, 2657, 2660, 2669, 3266
MmeI TCCRAC 2 cut(s) 345, 1397
Mox20I TGGCCA 1 cut(s) 1897
MroI TCCGGA 1 cut(s) 2769
MroXI GAANNNNTTC 1 cut(s) 2574
MscI TGGCCA 1 cut(s) 1897
MslI CAYNNNNRTG 5 cut(s) 542, 1491, 1601, 1859, 2532
Msp20I TGGCCA 1 cut(s) 1897
MspA1I CMGCKG 4 cut(s) 197, 278, 2221, 3754
MspCI CTTAAG 2 cut(s) 3653, 3674
MspI CCGG 4 cut(s) 230, 2325, 2619, 2770
MspR9I CCNGG 5 cut(s) 846, 2711, 3172, 3290, 3483
MunI CAATTG 1 cut(s) 3566
Mva1269I GAATGC 1 cut(s) 1056
MvaI CCWGG 5 cut(s) 846, 2711, 3172, 3290, 3483
MvnI CGCG 3 cut(s) 197, 278, 1516
NcoI CCATGG 1 cut(s) 87
NlaIV GGNNCC 3 cut(s) 843, 1237, 3176
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 4 cut(s) 583, 1861, 2630, 2825
NspI RCATGY 3 cut(s) 1573, 1600, 2198
OliI CACNNNNGTG 1 cut(s) 1859
PaeI GCATGC 2 cut(s) 1573, 2198
PagI TCATGA 3 cut(s) 853, 1255, 2719
PaqCI CACCTGC 1 cut(s) 2365
PceI AGGCCT 2 cut(s) 302, 3370
PciI ACATGT 1 cut(s) 1596
PciSI GCTCTTC 1 cut(s) 1183
PctI GAATGC 1 cut(s) 1056
PdmI GAANNNNTTC 1 cut(s) 2574
PfoI TCCNGGA 2 cut(s) 844, 3170
PinAI ACCGGT 1 cut(s) 2618
PleI GAGTC 8 cut(s) 444, 542, 1685, 1998, 2657, 2659, 2669, 3265
PmaCI CACGTG 1 cut(s) 1988
PmlI CACGTG 1 cut(s) 1988
PpsI GAGTC 8 cut(s) 444, 542, 1685, 1998, 2657, 2659, 2669, 3265
Ppu21I YACGTR 1 cut(s) 1988
PpuMI RGGWCCY 1 cut(s) 7
PscI ACATGT 1 cut(s) 1596
Psp5II RGGWCCY 1 cut(s) 7
Psp6I CCWGG 5 cut(s) 844, 2709, 3170, 3288, 3481
PspCI CACGTG 1 cut(s) 1988
PspFI CCCAGC 1 cut(s) 2819
PspGI CCWGG 5 cut(s) 844, 2709, 3170, 3288, 3481
PspN4I GGNNCC 3 cut(s) 843, 1237, 3176
PspPI GGNCC 3 cut(s) 7, 1982, 3037
PspPPI RGGWCCY 1 cut(s) 7
PstI CTGCAG 4 cut(s) 1928, 2362, 2971, 3270
PstNI CAGNNNCTG 2 cut(s) 2270, 3260
PsuI RGATCY 3 cut(s) 1235, 2793, 3630
PvuII CAGCTG 2 cut(s) 2221, 3754
RsaI GTAC 3 cut(s) 526, 1368, 3612
RsaNI GTAC 3 cut(s) 525, 1367, 3611
RseI CAYNNNNRTG 5 cut(s) 542, 1491, 1601, 1859, 2532
SacII CCGCGG 2 cut(s) 198, 279
SapI GCTCTTC 1 cut(s) 1183
Sau96I GGNCC 3 cut(s) 7, 1982, 3037
SchI GAGTC 8 cut(s) 444, 542, 1685, 1998, 2657, 2660, 2669, 3266
ScrFI CCNGG 5 cut(s) 846, 2711, 3172, 3290, 3483
SduI GDGCHC 3 cut(s) 652, 1005, 1921
SfcI CTRYAG 5 cut(s) 1478, 1924, 2358, 2967, 3266
Sfr303I CCGCGG 2 cut(s) 198, 279
SgrBI CCGCGG 2 cut(s) 198, 279
SinI GGWCC 3 cut(s) 7, 1982, 3037
SmiMI CAYNNNNRTG 5 cut(s) 542, 1491, 1601, 1859, 2532
SmlI CTYRAG 5 cut(s) 347, 1391, 1912, 3653, 3674
SmoI CTYRAG 5 cut(s) 347, 1391, 1912, 3653, 3674
SpeI ACTAGT 2 cut(s) 670, 722
SphI GCATGC 2 cut(s) 1573, 2198
SseBI AGGCCT 2 cut(s) 302, 3370
SspI AATATT 4 cut(s) 1087, 3024, 3357, 3471
StuI AGGCCT 2 cut(s) 302, 3370
StyD4I CCNGG 5 cut(s) 844, 2709, 3170, 3288, 3481
StyI CCWWGG 4 cut(s) 87, 303, 3045, 3371
TaaI ACNGT 6 cut(s) 419, 941, 1064, 1528, 2636, 3594
TaiI ACGT 3 cut(s) 243, 1990, 2114
TaqI TCGA 6 cut(s) 631, 1037, 2002, 2007, 2083, 2242
TatI WGTACW 1 cut(s) 1366
TauI GCSGC 9 cut(s) 77, 80, 143, 197, 200, 216, 278, 293, 3504
TseFI GTSAC 4 cut(s) 583, 1861, 2630, 2825
TseI GCWGC 9 cut(s) 200, 534, 553, 1942, 2175, 2221, 2398, 3137, 3265
Tsp45I GTSAC 4 cut(s) 583, 1861, 2630, 2825
TspGWI ACGGA 5 cut(s) 159, 326, 2483, 2806, 3628
Vha464I CTTAAG 2 cut(s) 3653, 3674
VpaK11BI GGWCC 3 cut(s) 7, 1982, 3037
XagI CCTNNNNNAGG 1 cut(s) 3375
XbaI TCTAGA 3 cut(s) 473, 835, 3073
XceI RCATGY 3 cut(s) 1573, 1600, 2198
XmiI GTMKAC 1 cut(s) 2479
XmnI GAANNNNTTC 1 cut(s) 2574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.