Rh6BG173800

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010240
Physical Location & Seq
Reverse (-)
28658446 .. 28666801
8356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG173800.1

Sequence Viewer

Length: 3819 bp
ATGGAGGTAGAATCGAATGCCCTCAAACCTATCCAGATGCAGAAGAAAAGAAAAGCTTCTGAAGAAACCAAACCCCCAAAACCAATGGACGCAGAGACCTCCACCACCGTCAAACCCATGGAGAAACAGAAGAAGCGAAAAGCTATGGACAAAGAGAGACGCCTCCACGCCGCAGCGCCAAAACCACCACCGTCCACGGATGATGTAGTGGAATTGAAAACCACCGAGCCACCCGCCGCCGCCGCCTCTTCTTCTAGCGGCGGAGCTCCTCTTCTTCCGGAGTTCCATGTCGGCGTGTTCAAGGACTTGGCTTCTGGTGATGCCGCGGTGAGAGAAGCGGCGGTGGAGGCTTTGGCGACGGAGCTGATGGAAGTGCAGAGAGCTTACGAGGGGCTTGAGAATAAGGAGTTGGTTGAAGGCGGGGGAGTCAAGTTGGAGGCCGAGAAGGACGATGGCTTGAATGACTGTGCGCCGTCGTTGAGATATGCTCTGCGGAGGCTCATTCGTGGTGTTTCTTCATCGAGAGAGTGTGCAAGACAAGGATTTGCATTAGGTTTAACTATTTTAGTAAGTACCATTCGCAGCATCAAGGTGGACTCATTGCTGAAACTCATAGTTGATTTCTTAGAGGTCACTTCATCAATGAAGGGTCAGGAGCAAAGGGATCGTCTTTTGGGTCGTTTATTTGCTTATGGTGCTCTTGGACGATCAGGAAGACTAGCTGAGGAGTGGATTTCTGACAGAAATACTCCGCACATAAAGGAATTCACTAGTCTTCTCATTGCTCTTGCATCAAAGAAGAGATACTTGCAAGAGCCTGCTGTATCAATTATTTTAGACTCGATTGAAAAGTTGCCCCTAGAAGCTTTGTTGACTCATGTTCTAGAAGCTCCAGGACTTCATGAATGGTTTGAAGGGGCTATTGAAATTGGAAATCCTGATGCTTTGCTTTTGGCTCTGAAAATTGGAGAAAAACTTTCTGTGGACAGTGCAAGATTTGGGAAACTTCTGCCAGACCCATTTGTTCCAAACAAACTGTTCTCTGCTGATCATCTATCTTCTCTTGCTAATAGTTTAAAGGAATCTACCTTCTGTCAGCCACGCATTCACAATGTTTGGCCTGTTCTAGTAAATATTCTGTTGCCAGATAGAGTTTTGCAGGCTGAAGATGCACTGACTATTTCAAATTCCTTAAAGAAGCACAAAAAGAATCGCAAGTCTAGCTCCTCTGATGAAGATATTGCAAAGAATTTCCAGTGCTTCTGCGAGGTGATTATTGAGGGATCCCTTCTCCCATCATCCCATGATCGCAAACACTTGGCCTTTGATGTTCTGCTTCTTCTCCTACCGAGGTTGCCTGCTTCTTACATACCAATTTGTTTGTCTTACAAAGTTGTTCAGTGCATGATTGATGTACTTCCAACAATGGATGCTTGGCTCAAGAAAATTGTGCAAAATTTTATTAAGACATTATCAGAATGGGTCGGGGATGATGATGTCAGAAGGGTTTCTGTCATTATGGCTCTACAGAAACACAGCAATGGTAGATTTGATTGTATCACACGAACAAAAACAGTTAAGGATTTGATGGCAGACTTCAGAACAGAATCGGGTTGCATGCTGTTTATTCAGAACTTGTTGAACATGTTCGTGGATGAAAGTCATGCTTCTGATGAACCTTCAGATCAGAGTATAACAACAGATGACAATTCTGAGATAGGTTCTATTGAAGACAAGGACTCTGTTGCAATGGGAAATTCTGATTTTTTGAAAACATGGATAGTGGAATCCCTCCCCTGTATATTAAAAAATTTAAAGCTAGAACCAGAAGCTAAGTTTAGGGTTCAAAAAGAAATTTTGAAGTTTCTAGCTGTTCAGGGTTTGTTCACTGCATCTCTTGGCACTGAAGTGACATCCTTTGAATTACAAGAGAAATTTAGGTGGCCAAAAGCAGCCACCTCAAGTGCTCTTTGCAGGATGTGTATTGAGCAGCTCCAGTTACTGTTGGCAAATTCTCAGAAAGGAGAAGGTCCACGTGGTTTGCCAAATCGACTCGAGTCAAATGATCTTGGCTCATATTTTATGCGGTTTCTTAGCACTTTGTGCAACATTCCTTCAATTTCTCTCTTTCGACCTTTGGATACCGAGGAAGAAAACACTTTGAAAAAATTGCAAGCGATGGAAACTTCACTTTCCAAAGAGGAAAGGAATTGTGGGCTTAGCAGCGAGGCAAATAGGTTGCATGCACTAAGATACTTGCTCATCCAGCTGCTTTTGCAAATGCTCCTTCGACCGAAAGAATTCTTAGTAGCGGTGTCTGAGCTTATTATATGCTGTAAAAAGGCTTTCCCCGTTTTTGATCTTGTTGATTCCTGTGGAGAAGACAACTTAGATGGTGATGACGCTCCTGCAGTAATGGATGTTCTTGTGGATACATTGCTTTCATTGCTGCCACAGTCATCAGCTCCTATGCGAACTGCTATTGAGCAGGTCTTTAAATACTTCTGTGTTGACATCACGGATGATGGTCTGCTGCGGATGTTGAGGGTCATTAGGAAGAATTTGAAACCTGCTAGACATCAGGATGCTGATAGTGAAGACATTGACGATGACGAAGATGAAGATTTTCTTAACATTGAAGAAGATGAGGTCATTGATAGAGCTGAGACTGGTGAAACAGGTGACAGTGATGAGCAGACAGACGAGTCTGAGGCCGACTCTGAGGCTGACTCTGAGGCTGTTGATGGTGTTGGGGAAGTTCCCATGGAAATTCCTGACGCTTCTGATGAATCAGATGGAGGGATGGATGATGATGCAATGTTCCGGATGGATACTTATCTTGCACGGATCTTCAAAGAGCGAAAGAATCTAGCTGGGGGTGACACTGCTCATCAGCAACTCATGCTGTTTAAGCTTCGAGTTCTTTCTTTGCTAGAAATTTACCTGCATGAAAATCCAGATAAGCCACAGGTTTTGTTGGTTTACTCAAATTTGGCTCGCGCGTTTGCTGAACCGCATACTGCAGAAAGTAGTGAACAGCTTGGACAGCGTATATGGGGAATTCTGCAAAAGAAAATATTTAAAGCAAAGGACCATCCCAAGGGTGAAGATGTGGAACTATCTACTCTAGAATCTTTGTTGCAAAGAAATTTGAAGTTGGCTTCAAAACCAATCAAGAGAAAGAAATCTGCTGCCAACCCGTCAAAGAAGAAACAGTCGGCCTCCTGGAACCGACAGAAAATGATTGCTTCCCTTGCACAGAGTTCTACCTTCTGGATTCTGAAGATCATTGATGCAAGAAATTTTCCAGAGTCTGAGCTGCAGAGAGTTTTTGATATTTTCCAGGGTGTTCTGGTAGAATATTTCAATAGTAAGAAGTCCCAAATCAAATCTGAATTTTTGAAAGAAATATTTAGAAGAAGGCCTTGGATAGGGCGTTATCTCTTTGGCTTCCTTTTGGAGAAATGCGGAAGTTCAAAGTCAGACTTTCGGCGAGTAGAAGCACTCGATATGGTGAGTGAAATATTGAAGTCCCCGGGCTTATCTGATGGAAGTGGCCAGGAAGCTTTGAAGAAAATTATGAAAAGCCATCTGCAAAAACTCTGTCAATTGATAGAGCAATTGCTTACAAATATGCCAGAAAAGCAGTCAAGACGAGCTGAAGTACGGAAGTTTTGCGGGAAGATCTTTCAGATGATAGCAACCCTTAAGCTGAGCAAGTCTTTTCTCAAGAATTTAGCTCCAGATGCTCATGCCAAGTGTGAATCACAACTTGGTGATCAGTTTAAGAACTTGAAGAAGATACAGGTGAAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1272

Amino Acids

142.67

Weight (kDa)

5.72

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 162 - 982 4.1e-179 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2704
Acc36I ACCTGC 3 cut(s) 2479, 2578, 2952
AccII CGCG 3 cut(s) 326, 3000, 3002
AccIII TCCGGA 2 cut(s) 277, 2823
AclWI GGATC 4 cut(s) 672, 1278, 1291, 2855
AcoI YGGCCR 2 cut(s) 1943, 3556
AcuI CTGAAG 7 cut(s) 81, 1185, 1582, 1665, 1926, 3302, 3681
AcvI CACGTG 1 cut(s) 2036
AcyI GRCGYC 1 cut(s) 160
AdeI CACNNNGTG 1 cut(s) 2103
AfaI GTAC 3 cut(s) 574, 1416, 3666
AfiI CCNNNNNNNGG 2 cut(s) 3100, 3431
AflII CTTAAG 1 cut(s) 3707
AflIII ACRYGT 1 cut(s) 1644
AhlI ACTAGT 1 cut(s) 770
AjnI CCWGG 4 cut(s) 892, 3224, 3342, 3558
AjuI GAANNNNNNNTTGG 6 cut(s) 392, 424, 3409, 3441, 3756, 3788
AleI CACNNNNGTG 1 cut(s) 1907
Alw21I GWGCWC 3 cut(s) 268, 700, 1969
Alw26I GTCTC 3 cut(s) 89, 151, 2660
AlwI GGATC 4 cut(s) 672, 1278, 1291, 2855
AlwNI CAGNNNCTG 2 cut(s) 2002, 3314
Ama87I CYCGRG 2 cut(s) 2054, 3535
Aor13HI TCCGGA 2 cut(s) 277, 2823
AoxI GGCC 8 cut(s) 438, 1118, 1320, 1943, 2712, 3219, 3422, 3556
ArsI GACNNNNNNTTYG 4 cut(s) 652, 684, 3589, 3621
Asp700I GAANNNNTTC 5 cut(s) 55, 1507, 1646, 2300, 2625
AspLEI GCGC 3 cut(s) 178, 472, 3002
AspS9I GGNCC 2 cut(s) 2030, 3091
AsuC2I CCSGG 2 cut(s) 3536, 3537
AvaI CYCGRG 2 cut(s) 2054, 3535
AvaII GGWCC 2 cut(s) 2030, 3091
BalI TGGCCA 2 cut(s) 1945, 3558
BamHI GGATCC 1 cut(s) 1283
BanII GRGCYC 1 cut(s) 268
BbrPI CACGTG 1 cut(s) 2036
BbsI GAAGAC 5 cut(s) 721, 767, 1737, 2388, 2604
Bbv12I GWGCWC 3 cut(s) 268, 700, 1969
BbvCI CCTCAGC 1 cut(s) 723
BceAI ACGGC 1 cut(s) 457
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 4 cut(s) 894, 3226, 3344, 3560
BciVI GTATCC 3 cut(s) 2134, 2425, 2824
BclI TGATCA 2 cut(s) 1048, 3778
BcnI CCSGG 2 cut(s) 3536, 3537
BcoDI GTCTC 3 cut(s) 89, 151, 2660
BcuI ACTAGT 1 cut(s) 770
BfmI CTRYAG 4 cut(s) 1526, 2409, 3021, 3320
BfoI RGCGCY 1 cut(s) 179
BfrI CTTAAG 1 cut(s) 3707
BfuAI ACCTGC 3 cut(s) 2479, 2578, 2952
BfuI GTATCC 3 cut(s) 2134, 2425, 2824
BglII AGATCT 1 cut(s) 3684
BlpI GCTNAGC 2 cut(s) 2219, 3713
Bme1390I CCNGG 6 cut(s) 894, 3226, 3344, 3536, 3537, 3560
Bme18I GGWCC 2 cut(s) 2030, 3091
BmeT110I CYCGRG 2 cut(s) 2054, 3535
BmgT120I GGNCC 2 cut(s) 2030, 3091
BmiI GGNNCC 2 cut(s) 1285, 3230
BmrFI CCNGG 6 cut(s) 894, 3226, 3344, 3536, 3537, 3560
BoxI GACNNNNGTC 1 cut(s) 2056
BpiI GAAGAC 5 cut(s) 721, 767, 1737, 2388, 2604
BplI GAGNNNNNCTC 6 cut(s) 472, 504, 2702, 2734, 2714, 2746
BpmI CTGGAG 3 cut(s) 876, 1979, 3726
Bpu10I CCTNAGC 1 cut(s) 723
Bpu1102I GCTNAGC 2 cut(s) 2219, 3713
BpuEI CTTGAG 4 cut(s) 416, 1424, 1945, 3713
BpuMI CCSGG 2 cut(s) 3536, 3537
BsaAI YACGTR 1 cut(s) 2036
BsaBI GATNNNNATC 1 cut(s) 2835
BsaHI GRCGYC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 89
BsaWI WCCGGW 2 cut(s) 277, 2823
Bsc4I CCNNNNNNNGG 2 cut(s) 3100, 3431
Bse1I ACTGG 3 cut(s) 1255, 1996, 2674
Bse3DI GCAATG 7 cut(s) 599, 780, 1546, 1755, 2435, 2444, 2823
Bse8I GATNNNNATC 1 cut(s) 2835
BseAI TCCGGA 2 cut(s) 277, 2823
BseBI CCWGG 4 cut(s) 894, 3226, 3344, 3560
BseJI GATNNNNATC 1 cut(s) 2835
BseLI CCNNNNNNNGG 2 cut(s) 3100, 3431
BseMI GCAATG 7 cut(s) 599, 780, 1546, 1755, 2435, 2444, 2823
BseNI ACTGG 3 cut(s) 1255, 1996, 2674
BseRI GAGGAG 3 cut(s) 258, 740, 1216
BseYI CCCAGC 1 cut(s) 2873
BsgI GTGCAG 1 cut(s) 395
Bsh1236I CGCG 3 cut(s) 326, 3000, 3002
Bsh1285I CGRYCG 1 cut(s) 2294
BshFI GGCC 8 cut(s) 440, 1120, 1322, 1945, 2714, 3221, 3424, 3558
BsiEI CGRYCG 1 cut(s) 2294
BsiHKAI GWGCWC 3 cut(s) 268, 700, 1969
BsiHKCI CYCGRG 2 cut(s) 2054, 3535
BsiSI CCGG 3 cut(s) 278, 2824, 3536
BslFI GGGAC 2 cut(s) 3364, 3517
BslI CCNNNNNNNGG 2 cut(s) 3100, 3431
BsmAI GTCTC 3 cut(s) 89, 151, 2660
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 2 cut(s) 3364, 3517
BsmI GAATGC 2 cut(s) 22, 1104
BsnI GGCC 8 cut(s) 440, 1120, 1322, 1945, 2714, 3221, 3424, 3558
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 2 cut(s) 2054, 3535
Bsp1286I GDGCHC 3 cut(s) 268, 700, 1969
Bsp13I TCCGGA 2 cut(s) 277, 2823
Bsp1720I GCTNAGC 2 cut(s) 2219, 3713
Bsp19I CCATGG 2 cut(s) 117, 2763
BspANI GGCC 8 cut(s) 440, 1120, 1322, 1945, 2714, 3221, 3424, 3558
BspEI TCCGGA 2 cut(s) 277, 2823
BspFNI CGCG 3 cut(s) 326, 3000, 3002
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 2 cut(s) 1285, 3230
BspMAI CTGCAG 3 cut(s) 2413, 3025, 3324
BspMI ACCTGC 3 cut(s) 2479, 2578, 2952
BspPI GGATC 4 cut(s) 672, 1278, 1291, 2855
BspTI CTTAAG 1 cut(s) 3707
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 7 cut(s) 599, 780, 1546, 1755, 2435, 2444, 2823
BsrI ACTGG 3 cut(s) 1255, 1996, 2674
BssNI GRCGYC 1 cut(s) 160
BssT1I CCWWGG 4 cut(s) 117, 2763, 3099, 3425
Bst2UI CCWGG 4 cut(s) 894, 3226, 3344, 3560
Bst6I CTCTTC 3 cut(s) 253, 276, 794
BstACI GRCGYC 1 cut(s) 160
BstAFI CTTAAG 1 cut(s) 3707
BstAPI GCANNNNNTGC 2 cut(s) 2103, 2902
BstBAI YACGTR 1 cut(s) 2036
BstC8I GCNNGC 7 cut(s) 819, 1161, 1359, 1619, 2175, 2244, 2998
BstDSI CCRYGG 4 cut(s) 117, 195, 324, 2763
BstENI CCTNNNNNAGG 1 cut(s) 3429
BstFNI CGCG 3 cut(s) 326, 3000, 3002
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 3 cut(s) 178, 472, 3002
BstMAI GTCTC 3 cut(s) 89, 151, 2660
BstMCI CGRYCG 1 cut(s) 2294
BstNI CCWGG 4 cut(s) 894, 3226, 3344, 3560
BstNSI RCATGY 3 cut(s) 1621, 1648, 2246
BstPAI GACNNNNGTC 1 cut(s) 2056
BstSCI CCNGG 6 cut(s) 892, 3224, 3342, 3534, 3535, 3558
BstSFI CTRYAG 4 cut(s) 1526, 2409, 3021, 3320
BstUI CGCG 3 cut(s) 326, 3000, 3002
BstV2I GAAGAC 5 cut(s) 721, 767, 1737, 2388, 2604
BstX2I RGATCY 3 cut(s) 1283, 2847, 3684
BstYI RGATCY 3 cut(s) 1283, 2847, 3684
BsuI GTATCC 3 cut(s) 2134, 2425, 2824
BsuRI GGCC 8 cut(s) 440, 1120, 1322, 1945, 2714, 3221, 3424, 3558
BtgI CCRYGG 4 cut(s) 117, 195, 324, 2763
BtgZI GCGATG 1 cut(s) 2192
BtsI GCAGTG 2 cut(s) 1887, 2883
BtsIMutI CAGTG 8 cut(s) 994, 1172, 1262, 1406, 1887, 1902, 2692, 2883
BveI ACCTGC 3 cut(s) 2479, 2578, 2952
Cac8I GCNNGC 7 cut(s) 819, 1161, 1359, 1619, 2175, 2244, 2998
CaiI CAGNNNCTG 2 cut(s) 2002, 3314
CciI TCATGA 1 cut(s) 901
CfoI GCGC 3 cut(s) 178, 472, 3002
Cfr13I GGNCC 2 cut(s) 2030, 3091
Cfr42I CCGCGG 1 cut(s) 327
Cfr9I CCCGGG 1 cut(s) 3535
CseI GACGC 4 cut(s) 98, 168, 2411, 2786
Csp6I GTAC 3 cut(s) 573, 1415, 3665
CspCI CAANNNNNGTGG 4 cut(s) 2022, 2057, 2955, 2990
CviQI GTAC 3 cut(s) 573, 1415, 3665
DraI TTTAAA 4 cut(s) 1077, 1815, 2497, 3082
DraIII CACNNNGTG 1 cut(s) 2103
DrdI GACNNNNNNGTC 1 cut(s) 2704
DseDI GACNNNNNNGTC 1 cut(s) 2704
EaeI YGGCCR 2 cut(s) 1943, 3556
Eam1104I CTCTTC 3 cut(s) 253, 276, 794
EarI CTCTTC 3 cut(s) 253, 276, 794
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 4 cut(s) 117, 2763, 3099, 3425
Eco147I AGGCCT 1 cut(s) 3424
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 2 cut(s) 2030, 3091
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 7 cut(s) 81, 1185, 1582, 1665, 1926, 3302, 3681
Eco72I CACGTG 1 cut(s) 2036
Eco88I CYCGRG 2 cut(s) 2054, 3535
EcoICRI GAGCTC 1 cut(s) 266
EcoNI CCTNNNNNAGG 1 cut(s) 3429
EcoRI GAATTC 3 cut(s) 764, 2300, 3060
EcoRII CCWGG 4 cut(s) 892, 3224, 3342, 3558
EcoT14I CCWWGG 4 cut(s) 117, 2763, 3099, 3425
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 4 cut(s) 117, 2763, 3099, 3425
Esp3I CGTCTC 1 cut(s) 151
FaqI GGGAC 2 cut(s) 3364, 3517
FauI CCCGC 3 cut(s) 241, 413, 3671
FbaI TGATCA 2 cut(s) 1048, 3778
FriOI GRGCYC 1 cut(s) 268
GlaI GCGC 3 cut(s) 177, 471, 3001
GsaI CCCAGC 1 cut(s) 2877
GsuI CTGGAG 3 cut(s) 876, 1979, 3726
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 8 cut(s) 440, 1120, 1322, 1945, 2714, 3221, 3424, 3558
HapII CCGG 3 cut(s) 278, 2824, 3536
HgaI GACGC 4 cut(s) 98, 168, 2411, 2786
HhaI GCGC 3 cut(s) 178, 472, 3002
Hin1I GRCGYC 1 cut(s) 160
Hin6I GCGC 3 cut(s) 176, 470, 3000
HinP1I GCGC 3 cut(s) 176, 470, 3000
HincII GTYRAC 2 cut(s) 873, 2512
HindII GTYRAC 2 cut(s) 873, 2512
HindIII AAGCTT 4 cut(s) 54, 864, 2912, 3564
HpaII CCGG 3 cut(s) 278, 2824, 3536
Hpy166II GTNNAC 9 cut(s) 195, 595, 873, 985, 1887, 2033, 2512, 2983, 3035
Hpy8I GTNNAC 9 cut(s) 195, 595, 873, 985, 1887, 2033, 2512, 2983, 3035
Hpy99I CGWCG 2 cut(s) 361, 478
HpyCH4IV ACGT 1 cut(s) 2035
HpySE526I ACGT 1 cut(s) 2035
Hsp92I GRCGYC 1 cut(s) 160
HspAI GCGC 3 cut(s) 176, 470, 3000
Kpn2I TCCGGA 2 cut(s) 277, 2823
Ksp22I TGATCA 2 cut(s) 1048, 3778
KspI CCGCGG 1 cut(s) 327
MaeII ACGT 1 cut(s) 2035
MaeIII GTNAC 5 cut(s) 631, 1909, 1998, 2681, 2879
MfeI CAATTG 2 cut(s) 3608, 3620
MflI RGATCY 3 cut(s) 1283, 2847, 3684
MhlI GDGCHC 3 cut(s) 268, 700, 1969
MlsI TGGCCA 2 cut(s) 1945, 3558
MluNI TGGCCA 2 cut(s) 1945, 3558
MmeI TCCRAC 2 cut(s) 414, 1445
Mox20I TGGCCA 2 cut(s) 1945, 3558
MroI TCCGGA 2 cut(s) 277, 2823
MroXI GAANNNNTTC 5 cut(s) 55, 1507, 1646, 2300, 2625
MscI TGGCCA 2 cut(s) 1945, 3558
MslI CAYNNNNRTG 5 cut(s) 590, 1539, 1649, 1907, 2583
Msp20I TGGCCA 2 cut(s) 1945, 3558
MspA1I CMGCKG 2 cut(s) 326, 2269
MspCI CTTAAG 1 cut(s) 3707
MspI CCGG 3 cut(s) 278, 2824, 3536
MspR9I CCNGG 6 cut(s) 894, 3226, 3344, 3536, 3537, 3560
MunI CAATTG 2 cut(s) 3608, 3620
Mva1269I GAATGC 2 cut(s) 22, 1104
MvaI CCWGG 4 cut(s) 894, 3226, 3344, 3560
MvnI CGCG 3 cut(s) 326, 3000, 3002
NciI CCSGG 2 cut(s) 3536, 3537
NcoI CCATGG 2 cut(s) 117, 2763
NlaIV GGNNCC 2 cut(s) 1285, 3230
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 4 cut(s) 631, 1909, 2681, 2879
NspI RCATGY 3 cut(s) 1621, 1648, 2246
OliI CACNNNNGTG 1 cut(s) 1907
PaeI GCATGC 2 cut(s) 1621, 2246
PaeR7I CTCGAG 1 cut(s) 2054
PagI TCATGA 1 cut(s) 901
PceI AGGCCT 1 cut(s) 3424
PciI ACATGT 1 cut(s) 1644
PctI GAATGC 2 cut(s) 22, 1104
PdmI GAANNNNTTC 5 cut(s) 55, 1507, 1646, 2300, 2625
PfoI TCCNGGA 2 cut(s) 892, 3224
PmaCI CACGTG 1 cut(s) 2036
PmlI CACGTG 1 cut(s) 2036
Ppu21I YACGTR 1 cut(s) 2036
PscI ACATGT 1 cut(s) 1644
PshAI GACNNNNGTC 1 cut(s) 2056
Psp124BI GAGCTC 1 cut(s) 268
Psp6I CCWGG 4 cut(s) 892, 3224, 3342, 3558
PspCI CACGTG 1 cut(s) 2036
PspFI CCCAGC 1 cut(s) 2873
PspGI CCWGG 4 cut(s) 892, 3224, 3342, 3558
PspN4I GGNNCC 2 cut(s) 1285, 3230
PspPI GGNCC 2 cut(s) 2030, 3091
PspXI VCTCGAGB 1 cut(s) 2054
PsrI GAACNNNNNNTAC 2 cut(s) 2406, 2438
PstI CTGCAG 3 cut(s) 2413, 3025, 3324
PstNI CAGNNNCTG 2 cut(s) 2002, 3314
PsuI RGATCY 3 cut(s) 1283, 2847, 3684
PvuII CAGCTG 1 cut(s) 2269
RsaI GTAC 3 cut(s) 574, 1416, 3666
RsaNI GTAC 3 cut(s) 573, 1415, 3665
RseI CAYNNNNRTG 5 cut(s) 590, 1539, 1649, 1907, 2583
SacI GAGCTC 1 cut(s) 268
SacII CCGCGG 1 cut(s) 327
Sau96I GGNCC 2 cut(s) 2030, 3091
ScrFI CCNGG 6 cut(s) 894, 3226, 3344, 3536, 3537, 3560
SduI GDGCHC 3 cut(s) 268, 700, 1969
SfcI CTRYAG 4 cut(s) 1526, 2409, 3021, 3320
Sfr274I CTCGAG 1 cut(s) 2054
Sfr303I CCGCGG 1 cut(s) 327
SgrBI CCGCGG 1 cut(s) 327
SinI GGWCC 2 cut(s) 2030, 3091
SlaI CTCGAG 1 cut(s) 2054
SmaI CCCGGG 1 cut(s) 3537
SmiMI CAYNNNNRTG 5 cut(s) 590, 1539, 1649, 1907, 2583
SmlI CTYRAG 6 cut(s) 395, 1439, 1960, 2054, 3707, 3728
SmoI CTYRAG 6 cut(s) 395, 1439, 1960, 2054, 3707, 3728
SpeI ACTAGT 1 cut(s) 770
SphI GCATGC 2 cut(s) 1621, 2246
SseBI AGGCCT 1 cut(s) 3424
SspI AATATT 5 cut(s) 1135, 3078, 3362, 3411, 3525
SstI GAGCTC 1 cut(s) 268
StuI AGGCCT 1 cut(s) 3424
StyD4I CCNGG 6 cut(s) 892, 3224, 3342, 3534, 3535, 3558
StyI CCWWGG 4 cut(s) 117, 2763, 3099, 3425
TaiI ACGT 1 cut(s) 2038
TaqI TCGA 9 cut(s) 14, 521, 842, 2050, 2055, 2131, 2290, 2917, 3507
TaqII GACCGA 1 cut(s) 2308
TatI WGTACW 1 cut(s) 1414
TauI GCSGC 7 cut(s) 173, 239, 242, 245, 261, 326, 341
TscAI CASTG 8 cut(s) 994, 1179, 1262, 1406, 1894, 1909, 2692, 2890
TseFI GTSAC 4 cut(s) 631, 1909, 2681, 2879
Tsp45I GTSAC 4 cut(s) 631, 1909, 2681, 2879
TspGWI ACGGA 5 cut(s) 212, 374, 2534, 2860, 3682
TspMI CCCGGG 1 cut(s) 3535
TspRI CASTG 8 cut(s) 994, 1179, 1262, 1406, 1894, 1909, 2692, 2890
Vha464I CTTAAG 1 cut(s) 3707
VpaK11BI GGWCC 2 cut(s) 2030, 3091
XagI CCTNNNNNAGG 1 cut(s) 3429
XbaI TCTAGA 2 cut(s) 883, 3127
XceI RCATGY 3 cut(s) 1621, 1648, 2246
XhoI CTCGAG 1 cut(s) 2054
XmaI CCCGGG 1 cut(s) 3535
XmnI GAANNNNTTC 5 cut(s) 55, 1507, 1646, 2300, 2625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.