MD05G1049800.v1.1

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010240
Physical Location & Seq
Forward (+)
8643541 .. 8651093
7553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1049800.v1.1.491

Sequence Viewer

Length: 3825 bp
ATGGGCAGTAAAAAGAGAAGCTCCACTTCCATGGAAGCTGCGGCTGATGCCGTCGCTGACGACGGCGGCTTCAGTAATCTGAAGAAATCGAAGAAGGGCAAAACAAAGCAGGAGACAACAGAAGCTTCTGCCCCTTCTTCTTCTACCGCTCCCACAGCCGTCAAACCCATGGAGAGACAAAAGAAAAGAAAAGCTTTGGACAAAGTGAGGCGCCTCCACACTGAGGAGACCAAACCCAAAGAACCCAAAACAATGGAGGTGCCCGCTTCGTCCTCGACGAGTGGGGTTCTTCCAGAGTTCCATGTCGGTGTGTTTAAGGACTTGTCTTCGGCTGATGGGTCTGTTAGAGAAGCCGCGGCGGAGGCTTTGGCTATGGAGTTAGTGGCTGTGCAGAGAGCTTATGATGGGCTTGAGAATAAGGAGTTGGTTGAGGGTGGAGTGAAGTTGGATGCCGAGAAGGATGATGGGTTGAATGATTGTGCGCCTTCCTTGAGATATGCTGTGCGGAGACTCATTCGCGGCGTTTCTTCATCAAGAGAGTGTGCAAGACAAGGGTTTGCATTAGGTTTAAGTATTTTAGTAAGTACGATCCCCAGCATCAAGGTGAACTCATTGTTGAAACTTATTGTTGATTTCTTAGAGGTCTCTTCATCAATGAAGGGTCAGGAACAAAGGGATCATCTTTTGGGTCGCTTATTTGCTTATGGTGCTATTGCCCGATCAGGAAGACTAGCTGAGGAGTGGGTTTCTGATAGAAATACTCCTCTCATAAAGGAATTCACTAGTCCTCTCATTGCCCTTGCATCAAAGAAACGATACTTGCAAGAGCCTGTTGTTTCGGTTATTGTAGACTTGATCGAGAAGTTGCACTCAGAAGCTTTGCTGAATCACATTCTAGAGGCCCCAGGACTTAATGAGTGGTTTGAAGGAGCTATTCAAATTGGAAATCCTGATGCATTGCTTCTGGCTCTGAAAATCAGAGAAAAAACTCTTATTGAAAGTTCAAGTTTTGGGAACCTTTTGCCAGTTCCATTCAGCCCTGACAAACTGTTTTCTGCTGACCATCTGTCTTCTCTTGCTAACTGCTTGAAGGAATCCACCTTTTGTCAGCCTCGTGTTCACAATGTGTGGCCTGTTCTGGTAAACATGCTTTTACCTGATAGAGTTTTGCAGGCTGAAGATGCAATTTCTGTTTCAAATTCCTTGAAAAGGCCCAAAAAGAATCGCAAGTCTAGCTCTTCTGATGAAGAAATTGCAAAGAACTTCCAGTGTTTCTGTGAGGTTATCATTGAGGGATCGCTTCTAACATCATCTCATGACCGCAAACACTTGGCCTTCGATGTTCTGCTTCTTCTTCTACCAAAGTTGCCTGCATCTTTTATTTCGATTACTCTGTCATACAAGATTGTTCAGTGCATGATTGATATACTTTCTACAAAGGATTCTTGGCTTTATAAAGTTGTGCAACATTTTCTTAAAACTTTAACTGTTTGGGTTGGGGATGATGACGTGAGAAGGGTTTCTGTCATTGTGGCTCTACAGAAACATAGCAACGGAAAATTTGATGGCATTACACGAACAAAAACAGTTAAGGATTTGATGTCAGATTTCAGAACAGAATCTGGTTGCATGCTGTTCATTCAGAACCTGTTGAACATGTTTCTGGATGAAAAACATACTACTGAGGAGCCTTCAGATCAGAGTCAAACGACAGATGACAATTCAGATATAGGTTCTGTTGAAGACAAGGATTCTGTTGGAACCATGGGAAATTCTGATTTTTTGAAAACATGGATTGTGGAATCCCTCCCCAGTATATTGAAAAATTTGAAACTGGATCCTGAAGCAAAATTTAGGGTCCAAAAAGGAATTTTGAAGTTTCTAGCAGTTCAGGGTTTGTTCACCGCATCTCTTGGGTCTGAAGTAACATCCCTTGAATTACAAGAGACATTTAGGTGGCCAAAAACTGCCGTCTCAAGTGCCCTTTGCAGGATGTGTATTGAGCAGCTCCAGTTACTGTTTGCAAACTCTCAGAAAGGAGAGGGGTCAGGTCCCTTGCTAAATTGCGTAGAGCAAAGTGACCTTGGCTCATACTTTATGCGGTTCCTTAGCACTTTGTGCAGTATTCCTTCCGTCTCATATTTTCGGCCTTTGGAAACCGAGGAAGAAAACACTTTGAAAAAATTGCAAGCAATGGAAACTTCACTCTCAAAAGAGGAAAGGAATTGTCAGCTTACTAGTGATGCAAATAGGTTGCATGCACTAAGATACTTACTCATCCAGCTGCTTTTGCAAATGCTCCTTCGACCAAAGGAATACTTAGAAGCAGTCTCTGAGCTTATGATATGTTGTAAGAAAGCTTTTCCTGTTGCTGATCTTCTTGATTCCCCTGGAGAAGATGACTTGGATTATGAGGGTGCACCTGCCATGATGGATGTTCTTGTGGATACATTGCTTTCTTTGCTGCCACAGTCATCAGCTCCCATGCGAACTGCAATCGAGCAGGTTTTTAAATGCTTCTGTGATGATATCACGGATGATGGTCTGTTGCGGATGTTGAGGGTCATCAGGAAGAATTTGAAACCTGCTAGACATCAAGATGCTGATAGTGACGACATTTTTGACGATGAAGAAGATGATGAAGATTTTCTTAACATTGAAGAAGATGAGGGAATTGATAAGGCTGAGACAGGTGAAACAGGTGACAGTGATGAGCAGCCAGATGACTCTGAGGCCGACTCTGAGGCTGCTGATGCAGTTGAGGCAGTTGGCAAAGAAAATCCTGAAGCTTCTGATGATTCTGATGGAGGAATGGATGATGATGCAATGTTTCGAATGGATACTTACCTTACCCAGATCTTCAAAGAGCGAAAGAATCTAGCTGGGGGTGACACTGCACATCACCAACTCATGCTGTTTAAGCTTCGTGTTCTTTCATTGCTAGAAATTTACCTACATGAAAATCCAGGCAAGCCGCAGGTTTTGTTGGTGTACTCAAACTTAGCTCGGGCATTTATTGAACCACCCAGTGCAGAAAGTAGTGAGCAGCTAGGACAGCGTGTATGGGGGATAATGCAAAAGAAAATTTTTAAAGCAAAGGACTATCCGAAGGGTGAAGATGTGCAATTATCTACTTTAGAATCTTTGCTGCAAAAGAATCTGAAGTTGGCCTCAAAACCTATTAAGAGAAAGAAATCGGCAACCAACCTGTCCAAGAAGAAACAGTCAGCTTCATGGAACCGACACAAAATGATTACTAGCCTTGCACAGAGCTCCACTTTCTGGATTCTGAAGATCGTTGAAGCAAGAAATTTTCCGGAGTCTGAGCTGCAGAGGATTTTTGATATTTTCCAGGGAGTTCTAGTGGAGTATTTCAATAGTAAAAAATCTCAAATCAAATCTGGATTTTTGAAAGAAATATTTAGAAGAAGGCCATGGGTAGGGCACCATCTGTTTGGCTTCCTGTTGGAGAAATGTGGCACTTCAAAGTCAGATTTTCGGCGAGTAGAAGCACTTGATTTGGTGAGTGAAATATTGAAGTCCCTGGGCTCCACTGATGGAAGTAGCCAGGAAGCGCTGAAGAATATCATGAAAAGCCATCTGCCGAAACTCTGTCGTCTGATAGAACAATTGCTGACAAATATATCAGAAAAACAGTCAAGGCAGGCTGAAGCGCGGAAGTTTTGTAGCAGGATCCTTCAGATGATAACAACCCTTAAGCTGACAAAGTCTTTCCTTAAAAAATTATCCCCAGATGCTCATGCCAAGTGTGAATCGCAACCCAGTGCACAATTTATTAAAATGAAGACGGTACCACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1275

Amino Acids

142.35

Weight (kDa)

5.95

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 166 - 985 7.9e-177 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1455
AarI CACCTGC 1 cut(s) 2431
Acc36I ACCTGC 4 cut(s) 2431, 2494, 2593, 2968
Acc65I GGTACC 1 cut(s) 3811
AccB1I GGYRCC 4 cut(s) 210, 259, 3444, 3811
AccB7I CCANNNNNTGG 1 cut(s) 3282
AccBSI CCGCTC 1 cut(s) 149
AccI GTMKAC 1 cut(s) 849
AccII CGCG 3 cut(s) 356, 519, 3676
AccIII TCCGGA 1 cut(s) 3316
AclWI GGATC 7 cut(s) 583, 684, 1303, 1832, 1845, 3688, 3701
AcoI YGGCCR 1 cut(s) 1958
AcyI GRCGYC 1 cut(s) 211
AdeI CACNNNGTG 3 cut(s) 1126, 2118, 3029
AfaI GTAC 3 cut(s) 586, 2993, 3813
AfeI AGCGCT 1 cut(s) 3576
AfiI CCNNNNNNNGG 5 cut(s) 223, 1209, 1989, 3282, 3440
AflII CTTAAG 1 cut(s) 3716
AflIII ACRYGT 1 cut(s) 1656
AhdI GACNNNNNGTC 1 cut(s) 1066
AhlI ACTAGT 2 cut(s) 782, 2237
AjiI CACGTC 1 cut(s) 1510
AjnI CCWGG 6 cut(s) 904, 2389, 2965, 3351, 3543, 3567
AjuI GAANNNNNNNTTGG 4 cut(s) 407, 439, 3149, 3181
AleI CACNNNNGTG 1 cut(s) 3820
Alw21I GWGCWC 3 cut(s) 2422, 3275, 3790
Alw44I GTGCAC 2 cut(s) 2418, 3786
AlwI GGATC 7 cut(s) 583, 684, 1303, 1832, 1845, 3688, 3701
AlwNI CAGNNNCTG 4 cut(s) 1622, 1648, 2017, 2333
Ama87I CYCGRG 1 cut(s) 3006
Aor13HI TCCGGA 1 cut(s) 3316
Aor51HI AGCGCT 1 cut(s) 3576
AoxI GGCC 9 cut(s) 900, 1130, 1211, 1332, 1958, 2147, 2733, 3168, 3431
ApaLI GTGCAC 2 cut(s) 2418, 3786
ApeKI GCWGC 9 cut(s) 38, 2005, 2284, 2464, 2716, 2747, 3046, 3148, 3328
ArsI GACNNNNNNTTYG 2 cut(s) 2603, 2635
Asp700I GAANNNNTTC 2 cut(s) 1519, 2646
Asp718I GGTACC 1 cut(s) 3811
AspLEI GCGC 4 cut(s) 213, 484, 3577, 3676
AspS9I GGNCC 4 cut(s) 901, 1212, 1858, 2051
AsuHPI GGTGA 8 cut(s) 616, 1894, 2705, 2714, 2894, 2900, 3125, 3535
AsuII TTCGAA 1 cut(s) 2833
AvaI CYCGRG 1 cut(s) 3006
AvaII GGWCC 2 cut(s) 1858, 2051
BaeGI GKGCMC 5 cut(s) 264, 1984, 2422, 3447, 3790
BalI TGGCCA 1 cut(s) 1960
BamHI GGATCC 2 cut(s) 1837, 3693
BanI GGYRCC 4 cut(s) 210, 259, 3444, 3811
BanII GRGCYC 2 cut(s) 3275, 3551
BauI CACGAG 1 cut(s) 1113
BbsI GAAGAC 5 cut(s) 318, 733, 1062, 1749, 3812
Bbv12I GWGCWC 3 cut(s) 2422, 3275, 3790
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 9 cut(s) 25, 2017, 2271, 2451, 2728, 2734, 3058, 3135, 3315
BceAI ACGGC 4 cut(s) 35, 79, 143, 1955
BciT130I CCWGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
BciVI GTATCC 2 cut(s) 2440, 2833
BcuI ACTAGT 2 cut(s) 782, 2237
BfmI CTRYAG 2 cut(s) 1538, 3329
BfoI RGCGCY 2 cut(s) 214, 3578
BfrI CTTAAG 1 cut(s) 3716
BfuAI ACCTGC 4 cut(s) 2431, 2494, 2593, 2968
BfuI GTATCC 2 cut(s) 2440, 2833
BglII AGATCT 1 cut(s) 2856
Bme1390I CCNGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
Bme18I GGWCC 2 cut(s) 1858, 2051
BmeRI GACNNNNNGTC 1 cut(s) 1066
BmeT110I CYCGRG 1 cut(s) 3006
BmgBI CACGTC 1 cut(s) 1510
BmgT120I GGNCC 4 cut(s) 901, 1212, 1858, 2051
BmrFI CCNGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
BmrI ACTGGG 3 cut(s) 1806, 3021, 3777
BmuI ACTGGG 3 cut(s) 1806, 3021, 3777
BpiI GAAGAC 5 cut(s) 318, 733, 1062, 1749, 3812
BplI GAGNNNNNCTC 2 cut(s) 2723, 2755
BpmI CTGGAG 2 cut(s) 1994, 2412
Bpu10I CCTNAGC 2 cut(s) 735, 2108
Bpu14I TTCGAA 1 cut(s) 2833
BpuEI CTTGAG 3 cut(s) 431, 511, 1960
BsaHI GRCGYC 1 cut(s) 211
BsaI GGTCTC 2 cut(s) 221, 649
BsaWI WCCGGW 1 cut(s) 3316
BsaXI ACNNNNNCTCC 2 cut(s) 3348, 3378
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 1209, 1989, 3282, 3440
Bse1I ACTGG 7 cut(s) 1025, 1267, 1812, 1839, 2011, 3027, 3783
Bse3DI GCAATG 6 cut(s) 792, 956, 2199, 2450, 2832, 2936
BseAI TCCGGA 1 cut(s) 3316
BseBI CCWGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
BseLI CCNNNNNNNGG 5 cut(s) 223, 1209, 1989, 3282, 3440
BseMI GCAATG 6 cut(s) 792, 956, 2199, 2450, 2832, 2936
BseNI ACTGG 7 cut(s) 1025, 1267, 1812, 1839, 2011, 3027, 3783
BseRI GAGGAG 4 cut(s) 239, 752, 753, 1700
BseSI GKGCMC 5 cut(s) 264, 1984, 2422, 3447, 3790
BseXI GCAGC 9 cut(s) 25, 2017, 2271, 2451, 2728, 2734, 3058, 3135, 3315
BseYI CCCAGC 2 cut(s) 593, 2882
BsgI GTGCAG 4 cut(s) 410, 2140, 2880, 3051
Bsh1236I CGCG 3 cut(s) 356, 519, 3676
BshFI GGCC 9 cut(s) 902, 1132, 1213, 1334, 1960, 2149, 2735, 3170, 3433
BshNI GGYRCC 4 cut(s) 210, 259, 3444, 3811
BsiHKAI GWGCWC 3 cut(s) 2422, 3275, 3790
BsiHKCI CYCGRG 1 cut(s) 3006
BsiSI CCGG 1 cut(s) 3317
BslFI GGGAC 2 cut(s) 2037, 3526
BslI CCNNNNNNNGG 5 cut(s) 223, 1209, 1989, 3282, 3440
BsmBI CGTCTC 2 cut(s) 1978, 2140
BsmFI GGGAC 2 cut(s) 2037, 3526
BsnI GGCC 9 cut(s) 902, 1132, 1213, 1334, 1960, 2149, 2735, 3170, 3433
Bso31I GGTCTC 2 cut(s) 221, 649
BsoBI CYCGRG 1 cut(s) 3006
Bsp119I TTCGAA 1 cut(s) 2833
Bsp1286I GDGCHC 7 cut(s) 264, 1984, 2422, 3275, 3447, 3551, 3790
Bsp13I TCCGGA 1 cut(s) 3316
Bsp19I CCATGG 4 cut(s) 30, 168, 1764, 3434
BspANI GGCC 9 cut(s) 902, 1132, 1213, 1334, 1960, 2149, 2735, 3170, 3433
BspEI TCCGGA 1 cut(s) 3316
BspFNI CGCG 3 cut(s) 356, 519, 3676
BspHI TCATGA 2 cut(s) 1315, 3588
BspMAI CTGCAG 1 cut(s) 3333
BspMI ACCTGC 4 cut(s) 2431, 2494, 2593, 2968
BspPI GGATC 7 cut(s) 583, 684, 1303, 1832, 1845, 3688, 3701
BspQI GCTCTTC 1 cut(s) 1243
BspT104I TTCGAA 1 cut(s) 2833
BspT107I GGYRCC 4 cut(s) 210, 259, 3444, 3811
BspTI CTTAAG 1 cut(s) 3716
BspTNI GGTCTC 2 cut(s) 221, 649
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 6 cut(s) 792, 956, 2199, 2450, 2832, 2936
BsrI ACTGG 7 cut(s) 1025, 1267, 1812, 1839, 2011, 3027, 3783
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 1 cut(s) 1113
BssT1I CCWWGG 5 cut(s) 30, 168, 1764, 2083, 3434
Bst2BI CACGAG 1 cut(s) 1113
Bst2UI CCWGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
Bst4CI ACNGT 9 cut(s) 1050, 1489, 1588, 2019, 2472, 2708, 3225, 3657, 3811
Bst6I CTCTTC 2 cut(s) 652, 1243
BstACI GRCGYC 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 3716
BstAPI GCANNNNNTGC 1 cut(s) 2118
BstBI TTCGAA 1 cut(s) 2833
BstC8I GCNNGC 8 cut(s) 264, 1173, 1371, 1631, 2190, 2259, 2972, 3666
BstDSI CCRYGG 5 cut(s) 30, 168, 354, 1764, 3434
BstENI CCTNNNNNAGG 1 cut(s) 1207
BstFNI CGCG 3 cut(s) 356, 519, 3676
BstH2I RGCGCY 2 cut(s) 214, 3578
BstHHI GCGC 4 cut(s) 213, 484, 3577, 3676
BstNI CCWGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
BstNSI RCATGY 4 cut(s) 1150, 1633, 1660, 2261
BstSCI CCNGG 6 cut(s) 904, 2389, 2965, 3351, 3543, 3567
BstSFI CTRYAG 2 cut(s) 1538, 3329
BstSLI GKGCMC 5 cut(s) 264, 1984, 2422, 3447, 3790
BstUI CGCG 3 cut(s) 356, 519, 3676
BstV1I GCAGC 9 cut(s) 25, 2017, 2271, 2451, 2728, 2734, 3058, 3135, 3315
BstV2I GAAGAC 5 cut(s) 318, 733, 1062, 1749, 3812
BstX2I RGATCY 3 cut(s) 1837, 2856, 3693
BstXI CCANNNNNNTGG 3 cut(s) 31, 253, 3455
BstYI RGATCY 3 cut(s) 1837, 2856, 3693
BsuI GTATCC 2 cut(s) 2440, 2833
BsuRI GGCC 9 cut(s) 902, 1132, 1213, 1334, 1960, 2149, 2735, 3170, 3433
BtgI CCRYGG 5 cut(s) 30, 168, 354, 1764, 3434
BtrI CACGTC 1 cut(s) 1510
BtsI GCAGTG 1 cut(s) 2892
BtsIMutI CAGTG 8 cut(s) 219, 1274, 1418, 2713, 2892, 3034, 3552, 3790
BveI ACCTGC 4 cut(s) 2431, 2494, 2593, 2968
Cac8I GCNNGC 8 cut(s) 264, 1173, 1371, 1631, 2190, 2259, 2972, 3666
CaiI CAGNNNCTG 4 cut(s) 1622, 1648, 2017, 2333
CciI TCATGA 2 cut(s) 1315, 3588
CfoI GCGC 4 cut(s) 213, 484, 3577, 3676
Cfr13I GGNCC 4 cut(s) 901, 1212, 1858, 2051
Cfr42I CCGCGG 1 cut(s) 357
Csp6I GTAC 3 cut(s) 585, 2992, 3812
CviQI GTAC 3 cut(s) 585, 2992, 3812
DinI GGCGCC 1 cut(s) 212
DraI TTTAAA 2 cut(s) 2512, 3091
DraIII CACNNNGTG 3 cut(s) 1126, 2118, 3029
DriI GACNNNNNGTC 1 cut(s) 1066
EaeI YGGCCR 1 cut(s) 1958
Eam1104I CTCTTC 2 cut(s) 652, 1243
Eam1105I GACNNNNNGTC 1 cut(s) 1066
EarI CTCTTC 2 cut(s) 652, 1243
EciI GGCGGA 1 cut(s) 374
Ecl136II GAGCTC 1 cut(s) 3273
Eco130I CCWWGG 5 cut(s) 30, 168, 1764, 2083, 3434
Eco24I GRGCYC 2 cut(s) 3275, 3551
Eco31I GGTCTC 2 cut(s) 221, 649
Eco32I GATATC 1 cut(s) 2530
Eco47I GGWCC 2 cut(s) 1858, 2051
Eco47III AGCGCT 1 cut(s) 3576
Eco53kI GAGCTC 1 cut(s) 3273
Eco88I CYCGRG 1 cut(s) 3006
EcoICRI GAGCTC 1 cut(s) 3273
EcoNI CCTNNNNNAGG 1 cut(s) 1207
EcoO109I RGGNCCY 2 cut(s) 901, 2051
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 6 cut(s) 904, 2389, 2965, 3351, 3543, 3567
EcoRV GATATC 1 cut(s) 2530
EcoT14I CCWWGG 5 cut(s) 30, 168, 1764, 2083, 3434
EcoT22I ATGCAT 1 cut(s) 958
EcoT38I GRGCYC 2 cut(s) 3275, 3551
EgeI GGCGCC 1 cut(s) 212
EheI GGCGCC 1 cut(s) 212
ErhI CCWWGG 5 cut(s) 30, 168, 1764, 2083, 3434
Esp3I CGTCTC 2 cut(s) 1978, 2140
FalI AAGNNNNNCTT 8 cut(s) 10, 42, 178, 210, 2303, 2335, 2634, 2666
FaqI GGGAC 2 cut(s) 2037, 3526
FauI CCCGC 1 cut(s) 271
FblI GTMKAC 1 cut(s) 849
FriOI GRGCYC 2 cut(s) 3275, 3551
GlaI GCGC 4 cut(s) 212, 483, 3576, 3675
GsaI CCCAGC 2 cut(s) 597, 2886
GsuI CTGGAG 2 cut(s) 1994, 2412
HaeII RGCGCY 2 cut(s) 214, 3578
HaeIII GGCC 9 cut(s) 902, 1132, 1213, 1334, 1960, 2149, 2735, 3170, 3433
HapII CCGG 1 cut(s) 3317
HhaI GCGC 4 cut(s) 213, 484, 3577, 3676
Hin1I GRCGYC 1 cut(s) 211
Hin6I GCGC 4 cut(s) 211, 482, 3575, 3674
HinP1I GCGC 4 cut(s) 211, 482, 3575, 3674
HindIII AAGCTT 6 cut(s) 123, 192, 876, 2358, 2787, 2921
HpaII CCGG 1 cut(s) 3317
HphI GGTGA 8 cut(s) 616, 1894, 2705, 2714, 2894, 2900, 3125, 3535
Hpy166II GTNNAC 8 cut(s) 607, 850, 1120, 1144, 1902, 2420, 2992, 3788
Hpy8I GTNNAC 8 cut(s) 607, 850, 1120, 1144, 1902, 2420, 2992, 3788
Hpy99I CGWCG 3 cut(s) 56, 65, 280
HpyCH4III ACNGT 9 cut(s) 1050, 1489, 1588, 2019, 2472, 2708, 3225, 3657, 3811
HpyCH4IV ACGT 1 cut(s) 1509
HpySE526I ACGT 1 cut(s) 1509
Hsp92I GRCGYC 1 cut(s) 211
HspAI GCGC 4 cut(s) 211, 482, 3575, 3674
KasI GGCGCC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 3316
KpnI GGTACC 1 cut(s) 3815
KspI CCGCGG 1 cut(s) 357
LguI GCTCTTC 1 cut(s) 1243
LmnI GCTCC 9 cut(s) 26, 154, 929, 1687, 2013, 2304, 2485, 3278, 3554
Lsp1109I GCAGC 9 cut(s) 25, 2017, 2271, 2451, 2728, 2734, 3058, 3135, 3315
MaeII ACGT 1 cut(s) 1509
MaeIII GTNAC 6 cut(s) 1924, 2013, 2078, 2609, 2702, 2888
MbiI CCGCTC 1 cut(s) 149
MfeI CAATTG 1 cut(s) 3629
MflI RGATCY 3 cut(s) 1837, 2856, 3693
MhlI GDGCHC 7 cut(s) 264, 1984, 2422, 3275, 3447, 3551, 3790
MlsI TGGCCA 1 cut(s) 1960
MluNI TGGCCA 1 cut(s) 1960
Mly113I GGCGCC 1 cut(s) 211
MlyI GAGTC 5 cut(s) 504, 1711, 2720, 2732, 3329
MmeI TCCRAC 3 cut(s) 426, 1738, 3447
Mox20I TGGCCA 1 cut(s) 1960
Mph1103I ATGCAT 1 cut(s) 958
MroI TCCGGA 1 cut(s) 3316
MroXI GAANNNNTTC 2 cut(s) 1519, 2646
MscI TGGCCA 1 cut(s) 1960
MslI CAYNNNNRTG 6 cut(s) 29, 306, 602, 1954, 2598, 3820
Msp20I TGGCCA 1 cut(s) 1960
MspA1I CMGCKG 2 cut(s) 356, 2284
MspCI CTTAAG 1 cut(s) 3716
MspI CCGG 1 cut(s) 3317
MspR9I CCNGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
MunI CAATTG 1 cut(s) 3629
MvaI CCWGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
MvnI CGCG 3 cut(s) 356, 519, 3676
NarI GGCGCC 1 cut(s) 211
NcoI CCATGG 4 cut(s) 30, 168, 1764, 3434
NmeAIII GCCGAG 1 cut(s) 478
NmuCI GTSAC 4 cut(s) 2078, 2609, 2702, 2888
NsiI ATGCAT 1 cut(s) 958
NspI RCATGY 4 cut(s) 1150, 1633, 1660, 2261
NspV TTCGAA 1 cut(s) 2833
OliI CACNNNNGTG 1 cut(s) 3820
PaeI GCATGC 2 cut(s) 1633, 2261
PagI TCATGA 2 cut(s) 1315, 3588
PaqCI CACCTGC 1 cut(s) 2431
PasI CCCWGGG 1 cut(s) 3544
PciI ACATGT 1 cut(s) 1656
PciSI GCTCTTC 1 cut(s) 1243
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PdmI GAANNNNTTC 2 cut(s) 1519, 2646
PflFI GACNNNGTC 1 cut(s) 3727
PflMI CCANNNNNTGG 1 cut(s) 3282
PleI GAGTC 5 cut(s) 504, 1710, 2720, 2732, 3328
PluTI GGCGCC 1 cut(s) 214
PpsI GAGTC 5 cut(s) 504, 1710, 2720, 2732, 3328
PpuMI RGGWCCY 1 cut(s) 2051
PscI ACATGT 1 cut(s) 1656
PsiI TTATAA 1 cut(s) 1455
Psp124BI GAGCTC 1 cut(s) 3275
Psp5II RGGWCCY 1 cut(s) 2051
Psp6I CCWGG 6 cut(s) 904, 2389, 2965, 3351, 3543, 3567
PspFI CCCAGC 2 cut(s) 593, 2882
PspGI CCWGG 6 cut(s) 904, 2389, 2965, 3351, 3543, 3567
PspPI GGNCC 4 cut(s) 901, 1212, 1858, 2051
PspPPI RGGWCCY 1 cut(s) 2051
PstI CTGCAG 1 cut(s) 3333
PstNI CAGNNNCTG 4 cut(s) 1622, 1648, 2017, 2333
PsuI RGATCY 3 cut(s) 1837, 2856, 3693
PsyI GACNNNGTC 1 cut(s) 3727
PvuII CAGCTG 1 cut(s) 2284
RsaI GTAC 3 cut(s) 586, 2993, 3813
RsaNI GTAC 3 cut(s) 585, 2992, 3812
RseI CAYNNNNRTG 6 cut(s) 29, 306, 602, 1954, 2598, 3820
SacI GAGCTC 1 cut(s) 3275
SacII CCGCGG 1 cut(s) 357
SapI GCTCTTC 1 cut(s) 1243
Sau96I GGNCC 4 cut(s) 901, 1212, 1858, 2051
SchI GAGTC 5 cut(s) 504, 1711, 2720, 2732, 3329
ScrFI CCNGG 6 cut(s) 906, 2391, 2967, 3353, 3545, 3569
SduI GDGCHC 7 cut(s) 264, 1984, 2422, 3275, 3447, 3551, 3790
SfcI CTRYAG 2 cut(s) 1538, 3329
SfoI GGCGCC 1 cut(s) 212
Sfr303I CCGCGG 1 cut(s) 357
SfuI TTCGAA 1 cut(s) 2833
SgrBI CCGCGG 1 cut(s) 357
SinI GGWCC 2 cut(s) 1858, 2051
SmiMI CAYNNNNRTG 6 cut(s) 29, 306, 602, 1954, 2598, 3820
SmlI CTYRAG 4 cut(s) 410, 490, 1975, 3716
SmoI CTYRAG 4 cut(s) 410, 490, 1975, 3716
SpeI ACTAGT 2 cut(s) 782, 2237
SphI GCATGC 2 cut(s) 1633, 2261
SspDI GGCGCC 1 cut(s) 210
SspI AATATT 2 cut(s) 3420, 3534
SstI GAGCTC 1 cut(s) 3275
StyD4I CCNGG 6 cut(s) 904, 2389, 2965, 3351, 3543, 3567
StyI CCWWGG 5 cut(s) 30, 168, 1764, 2083, 3434
TaaI ACNGT 9 cut(s) 1050, 1489, 1588, 2019, 2472, 2708, 3225, 3657, 3811
TaiI ACGT 1 cut(s) 1512
TaqI TCGA 8 cut(s) 89, 275, 858, 1338, 1385, 2305, 2499, 2833
TatI WGTACW 1 cut(s) 2991
TauI GCSGC 6 cut(s) 44, 69, 356, 359, 522, 2977
TscAI CASTG 8 cut(s) 226, 1274, 1418, 2713, 2899, 3034, 3559, 3790
TseFI GTSAC 4 cut(s) 2078, 2609, 2702, 2888
TseI GCWGC 9 cut(s) 38, 2005, 2284, 2464, 2716, 2747, 3046, 3148, 3328
Tsp45I GTSAC 4 cut(s) 2078, 2609, 2702, 2888
TspGWI ACGGA 3 cut(s) 1569, 2122, 2549
TspRI CASTG 8 cut(s) 226, 1274, 1418, 2713, 2899, 3034, 3559, 3790
Tth111I GACNNNGTC 1 cut(s) 3727
Van91I CCANNNNNTGG 1 cut(s) 3282
Vha464I CTTAAG 1 cut(s) 3716
VneI GTGCAC 2 cut(s) 2418, 3786
VpaK11BI GGWCC 2 cut(s) 1858, 2051
XagI CCTNNNNNAGG 1 cut(s) 1207
XbaI TCTAGA 1 cut(s) 895
XceI RCATGY 4 cut(s) 1150, 1633, 1660, 2261
XmiI GTMKAC 1 cut(s) 849
XmnI GAANNNNTTC 2 cut(s) 1519, 2646
Zsp2I ATGCAT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.