MD10G1056900.v1.1

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010240
Physical Location & Seq
Forward (+)
7753189 .. 7761532
8344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1056900.v1.1.491

Sequence Viewer

Length: 3843 bp
ATGGGCAGTAAAAAGAGAAGCTCCACTTCCATGGAAGCTGCGGCTGATGCCGTCTTTGACGATGGCGGCTTCAGTAATCTGAAGAAATCGAAGAAGGGCAAAACAAAGCAGGAGACAGCAGAAGCTTCTGCCCCTTCTTCTTCTGCCGCTACCACCGCCGTCAAACCCATGGAGAGACAAAAGAAAAGAAAATCTTTGGACAAAGTGAGGCGCCTCCACACTGAGGAGACCAAACCCAAAGAACCTAAAACAGTGGAGGTTCAATTGACAACTGAGGAGCTCGCTTCGTCTTCGACGAGCGGGATTCTTCCGGAGTTCCATGTGGGTGTGTTTAAGGACTTGGCTTCGGGTGATGGGTCTGTTAGAGAAGCTGCGGCGGAGGCTTTGGCTATGGAGTTGGTGGCGGTGCAGAGAGCTTATGATGGGCTTGAGAATAAGGAGTTGGTTGAAGGTGGAGTGAAGATGGAAGCCGAGAAGGATGATGGGTTGAATGGTTGTGCGCCTTCCTTGAGATATGCCGTGCGGAGACTCATTCGCGGTGTTTCTTCATCAAGAGAGTGTGCAAGACAAGGGTTTGCATTAGGTTTAACTATTTTAGTAAGTACAATCCCCAGCATCAAGGTGAAGTCATTGTTGAAACTCATTGTTGATTTCTTAGAGGTTTCTTCATCAATGAAGGGTCAGGAACAAAGGGATCATCTTTTGGGTCGCTTATTTGCCTATGGAGCTATTGCCCGATCAGGAAGACTAGCTGAGGAGTGGGTTTCTGATAGAAATACTCCTCTCATAAAGGAATTCACTAGTCTTCTCATTGCCCTTGCATCAAAAAAGCGATACTTGCAGGAGCCTGTTGTTTCGGTTATTGTAGACTTGATTGAGAAGTTGCACTTGGAGGCCTTGCTGAACCACATGCTAGAGGCCCCAGGACTTAATGAGTGGTTTGAAGGAGCTATTCAAGTTGGAAATCCTGATGCATTGCTTCTGGCTCTGAAAATCAGAGAAAAAACTCTTGTTGAAAGTTCAAGTTTTGGGAACCTTTTACCAGTCCCATTCAGCCCCAACAAACTGTTTTCTGCTGACCATCTGTCTTCTCTTGCTAACTGCTTGAAGGAATCCACCTTTTGTCAGCCTCGTGTTCACAATGTGTGGCCTGTTCTGGTAAACATGCTTTTACCTGATAGAGTTTTGCAGGCTGAAGATGCAATTTCTGTTTCAAATTCCTTGAAGAGGCCTAAAAAGAATCGCAAGTCTAGCTCTTCTGATGAAGAAATTGCAAAGAATTTCCAGTGCTTCTGCGAGGTGGTCATGGAGGGATCACTTCTACCATCATCTCATGACCGCAAACACTTGGCCTTTGATGTTCTGCTTCTTCTTCTACCAAAGTTGCCTGCATCTTTTATTCCGATTAGTCTGTCTTACAAAATTGTTCAGTGCATGATTGACATACTTTCTACAAAGGATTCTTGGCTCTATAAAGTTGTGCAACATTTTCTTAAAACATTAACTGATTGGATTGGGGATGATGATGTGAGAAGGGTTTCTGTCATTGTGGCTTTACAGAAACATAGCAATGGAAAATTTGATTGCATTACACGAACAAAGACAGTTAAGGATTTGATGTCAGATTTCAGAACAGAATCTGGTTGCATGCTGTTCATTCAGAACCTGTTGAACATGTTTCTGGATGAAAAACATGCTACCGAGGAACCTTCAGATCAGAGTCAAACGACAGATGACAATTCAGAGATAGGTTCTGTTGAAGACAAGGATTCCGTTGGAACCATGGGAAATTCTGATTTTTTGAAAACATGGATTGTGGAATCCCTCCCTAGTATATTGAAAAATTTGAAACTGGATCCTGAAGCAAAGTTTAGGGTCCAAAAAGGAATTTTGAAGTTTCTAGCAGTTCAGGGATTGTTCACCGCATCCCTTGGGTCTGAAGTAACATCCCTTGAATTACAAGAGACATTTAGGTGGCCAAAAGCTGCCACCTCAAGTGCCCTTTGCAGGATGTGTATCGAGCAGCTTCAGTTACTGTTCGCAAACTCTCAGAAAGGAGAGGGGCCACGTCCTTTGCCAAATTGCATAGAGCAAATTGACCTTGGCTCATACTTTATGCGCTTTCTTTGCACTTTGTGCAGTATTCCTTCCGTCTCTTATTTTCGGCCTCTGGAAATTGAGGAAGAAAACACTTTGAAAAAATTGCAAGCAATGGAAACTTCACTCGCAAAAGAGGAAAGGAATTGTGGGCTTAGTAGTGATGCAAATAGGTTGCATGCACTAAGATACTTGCTCATCCAGCTGCTTTTGCAAATGCTCCTTCGACCAAAGGAATACTTAGAAGCAGTCTCCGAGCTTATTATATGTTGTAAGAAAGCTTTTCCTGTTGCTGATCTTCTTGATTCCCCTGGAGAAGATGACTTGGATGATGATGGTGCACCTGCCGTGATGGATGTTCTTGTGGATACATCGCTTTCTTTGCTGCCACAGTCATCAGCTCCCATGCGAACAGCAATTGAGCAGGTATTTAAATATTTCTGTGGTGATATCACGGATGATGGTCTGTTGCGGATGTTGAGGGTCATTAGGAAGAATTTGAAACCTGCTAGACATCAAGATGCTGATAGTGACGACATTTTTGACGATGAAGAAGATGATGAAGAATTTCTTAACATTGAAGAAGATGAGGGAATTGATAAAGCTGAGACAGGTGAAACCGGTGACAGTGATGAACAGCCAGATGACTCCGAGGCTGACTCTGAGGCTGTTGATGCATTTGAGGCAGTTGGCAAAGAAAGTCCTGAAGCTTCTGATGATTCTGATGGAGGAATGGATGATGATGCAATGTTTCGAATGGATACTTACCTTACCCAGATCTTCAAAGAGCGAAAGAATCTAGCTGGGGGTGACACTGCCCATCACCAACTCATGCTGTTTAAGCTTCGTGTTCTTTCATTGCTAGAAATTTACCTACATGAAAATCCAGGCAAGCCGCAGGTTTTGTTGGTGTACTCAAACTTAGCTCGGGCATTTATTGACCCACCCAGTGCAGAAAGTAGCGAACAGCTAGGACAGCGTGTATGGGGGATACTGCAAAAGAAAATATTTAAAGCAAAGGACTATCCGAAGGGTGAAGATGTGCAATTATCTACTTTAGAATCTTTGCTGCAAAAGAATTTGAAGTTGGCCTCAAAACCAATTAAGAGAAAGAAATCCGCAACCAACCTGTCCAAGAAGAAACAGTCAGCCTCGTGGAACCGACACAAAATGATTACTAGCCTTGCACAGAGCTCCACTTTCTGGATTCTGAAGATTGTTGAAGCAAGAAATTTTCCAGAGTCTGAGCTGCAGAGGGTTTTTGATATTTTCCAGGGAGTTCTAGTAGAGTATTTCAATAGTAAAAAGTCTCAAATCAAATCTGGATTTTTGAAAGAAATATTTAGAAGAAGGCCATGGGTAGGGCACCATCTCTTTGGCTTCCTTTTGGAGAAATGTGGCACTTCAAAGTCAGATTTTCGGCGAGTAGAAGCACTTGATTTGGTGAGTGAAATATTGAAGTCCCTGGGCTCTACTGATGGAAGTAGCCAGGAAGCGCTGAAGAATATTATGAAAAGCCATCTGGAGAAACTCTGTCGTCTGATAGAACAATTGCTGACAAACAAATCAGAAAAACAGTCGAGGCAGGCTGAAGCACGGAAGTTTTGTAGCAGGATTCTTCAGATGATAACAACCCTTAAGCTGACCAAGTCTTTCCTCAAAAATTTATCCCCAGATGCTCATGCCAAGTGTGAATCGCAACTCGGTGCCCAATTTACTAAAATGAAGACAGTAGCACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1281

Amino Acids

143.04

Weight (kDa)

5.95

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 172 - 991 4.5e-174 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2449
Acc36I ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
AccB1I GGYRCC 3 cut(s) 210, 3462, 3803
AccB7I CCANNNNNTGG 1 cut(s) 3300
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 310
AclWI GGATC 4 cut(s) 702, 1321, 1850, 1863
AcoI YGGCCR 1 cut(s) 1976
AcyI GRCGYC 1 cut(s) 211
AdeI CACNNNGTG 3 cut(s) 1144, 2136, 3047
AfaI GTAC 2 cut(s) 604, 3011
AfeI AGCGCT 1 cut(s) 3594
AfiI CCNNNNNNNGG 5 cut(s) 223, 1227, 2007, 3300, 3458
AflII CTTAAG 1 cut(s) 3734
AflIII ACRYGT 1 cut(s) 1674
AgeI ACCGGT 1 cut(s) 2717
AhdI GACNNNNNGTC 1 cut(s) 1084
AhlI ACTAGT 1 cut(s) 800
AjiI CACGTC 1 cut(s) 2069
AjnI CCWGG 6 cut(s) 922, 2407, 2983, 3369, 3561, 3585
AjuI GAANNNNNNNTTGG 6 cut(s) 425, 457, 872, 904, 3167, 3199
AleI CACNNNNGTG 1 cut(s) 3838
Alw21I GWGCWC 3 cut(s) 282, 2440, 3293
Alw26I GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3411
Alw44I GTGCAC 1 cut(s) 2436
AlwI GGATC 4 cut(s) 702, 1321, 1850, 1863
AlwNI CAGNNNCTG 4 cut(s) 1640, 1666, 2035, 3341
Ama87I CYCGRG 1 cut(s) 3024
Aor13HI TCCGGA 1 cut(s) 310
Aor51HI AGCGCT 1 cut(s) 3594
ApaLI GTGCAC 1 cut(s) 2436
ApeKI GCWGC 8 cut(s) 38, 371, 1985, 2023, 2302, 2482, 3166, 3346
ArsI GACNNNNNNTTYG 2 cut(s) 2621, 2653
AsiGI ACCGGT 1 cut(s) 2717
Asp700I GAANNNNTTC 2 cut(s) 1537, 2664
AspLEI GCGC 4 cut(s) 213, 502, 2121, 3595
AspS9I GGNCC 3 cut(s) 919, 1876, 2063
AsuII TTCGAA 1 cut(s) 2851
AvaI CYCGRG 1 cut(s) 3024
AvaII GGWCC 1 cut(s) 1876
BaeGI GKGCMC 4 cut(s) 2002, 2440, 3465, 3808
BalI TGGCCA 1 cut(s) 1978
BamHI GGATCC 1 cut(s) 1855
BanI GGYRCC 3 cut(s) 210, 3462, 3803
BanII GRGCYC 3 cut(s) 282, 3293, 3569
BauI CACGAG 2 cut(s) 1131, 3250
BbsI GAAGAC 6 cut(s) 282, 751, 797, 1080, 1767, 3830
Bbv12I GWGCWC 3 cut(s) 282, 2440, 3293
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 8 cut(s) 25, 358, 1972, 2035, 2289, 2469, 3153, 3333
BceAI ACGGC 4 cut(s) 35, 143, 503, 2429
BciT130I CCWGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
BciVI GTATCC 3 cut(s) 2458, 2851, 3081
BcoDI GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3411
BcuI ACTAGT 1 cut(s) 800
BfmI CTRYAG 1 cut(s) 3347
BfoI RGCGCY 2 cut(s) 214, 3596
BfrI CTTAAG 1 cut(s) 3734
BfuAI ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
BfuI GTATCC 3 cut(s) 2458, 2851, 3081
BglII AGATCT 1 cut(s) 2874
Bme1390I CCNGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
Bme18I GGWCC 1 cut(s) 1876
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 3024
BmgBI CACGTC 1 cut(s) 2069
BmgT120I GGNCC 3 cut(s) 919, 1876, 2063
BmrFI CCNGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
BmrI ACTGGG 1 cut(s) 3039
BmuI ACTGGG 1 cut(s) 3039
BpiI GAAGAC 6 cut(s) 282, 751, 797, 1080, 1767, 3830
BplI GAGNNNNNCTC 2 cut(s) 2741, 2773
BpmI CTGGAG 2 cut(s) 2430, 3641
Bpu10I CCTNAGC 1 cut(s) 753
Bpu14I TTCGAA 1 cut(s) 2851
BpuEI CTTGAG 3 cut(s) 449, 529, 1978
BsaBI GATNNNNATC 1 cut(s) 2015
BsaHI GRCGYC 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 221
BsaWI WCCGGW 2 cut(s) 310, 2717
BsaXI ACNNNNNCTCC 2 cut(s) 3366, 3396
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 1227, 2007, 3300, 3458
Bse118I RCCGGY 1 cut(s) 2717
Bse1I ACTGG 4 cut(s) 1043, 1285, 1857, 3045
Bse3DI GCAATG 6 cut(s) 810, 974, 1576, 2217, 2850, 2954
Bse8I GATNNNNATC 1 cut(s) 2015
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
BseJI GATNNNNATC 1 cut(s) 2015
BseLI CCNNNNNNNGG 5 cut(s) 223, 1227, 2007, 3300, 3458
BseMI GCAATG 6 cut(s) 810, 974, 1576, 2217, 2850, 2954
BseMII CTCAG 7 cut(s) 213, 264, 744, 2063, 2694, 2751, 3333
BseNI ACTGG 4 cut(s) 1043, 1285, 1857, 3045
BseRI GAGGAG 4 cut(s) 239, 290, 770, 771
BseSI GKGCMC 4 cut(s) 2002, 2440, 3465, 3808
BseXI GCAGC 8 cut(s) 25, 358, 1972, 2035, 2289, 2469, 3153, 3333
BseYI CCCAGC 2 cut(s) 611, 2900
BsgI GTGCAG 3 cut(s) 428, 2158, 3069
Bsh1236I CGCG 1 cut(s) 537
BshNI GGYRCC 3 cut(s) 210, 3462, 3803
BshTI ACCGGT 1 cut(s) 2717
BsiHKAI GWGCWC 3 cut(s) 282, 2440, 3293
BsiHKCI CYCGRG 1 cut(s) 3024
BsiSI CCGG 2 cut(s) 311, 2718
BslFI GGGAC 2 cut(s) 1031, 3544
BslI CCNNNNNNNGG 5 cut(s) 223, 1227, 2007, 3300, 3458
BsmAI GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3411
BsmBI CGTCTC 1 cut(s) 2158
BsmFI GGGAC 2 cut(s) 1031, 3544
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 3024
Bsp119I TTCGAA 1 cut(s) 2851
Bsp1286I GDGCHC 7 cut(s) 282, 2002, 2440, 3293, 3465, 3569, 3808
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
Bsp19I CCATGG 4 cut(s) 30, 168, 1782, 3452
BspCNI CTCAG 7 cut(s) 214, 265, 745, 2062, 2695, 2752, 3334
BspEI TCCGGA 1 cut(s) 310
BspFNI CGCG 1 cut(s) 537
BspHI TCATGA 1 cut(s) 1333
BspMAI CTGCAG 1 cut(s) 3351
BspMI ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
BspPI GGATC 4 cut(s) 702, 1321, 1850, 1863
BspQI GCTCTTC 1 cut(s) 1261
BspT104I TTCGAA 1 cut(s) 2851
BspT107I GGYRCC 3 cut(s) 210, 3462, 3803
BspTI CTTAAG 1 cut(s) 3734
BspTNI GGTCTC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 6 cut(s) 810, 974, 1576, 2217, 2850, 2954
BsrFI RCCGGY 1 cut(s) 2717
BsrI ACTGG 4 cut(s) 1043, 1285, 1857, 3045
BssAI RCCGGY 1 cut(s) 2717
BssMI GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 2 cut(s) 1131, 3250
BssT1I CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3452
Bst2BI CACGAG 2 cut(s) 1131, 3250
Bst2UI CCWGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
Bst4CI ACNGT 9 cut(s) 253, 1068, 1606, 2037, 2490, 2726, 3243, 3675, 3829
Bst6I CTCTTC 2 cut(s) 1220, 1261
BstACI GRCGYC 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 3734
BstAPI GCANNNNNTGC 1 cut(s) 2136
BstBI TTCGAA 1 cut(s) 2851
BstC8I GCNNGC 8 cut(s) 282, 1191, 1389, 1649, 2208, 2277, 2990, 3684
BstDSI CCRYGG 4 cut(s) 30, 168, 1782, 3452
BstENI CCTNNNNNAGG 1 cut(s) 1225
BstFNI CGCG 1 cut(s) 537
BstH2I RGCGCY 2 cut(s) 214, 3596
BstHHI GCGC 4 cut(s) 213, 502, 2121, 3595
BstKTI GATC 7 cut(s) 697, 740, 1316, 1717, 1858, 2395, 2877
BstMAI GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3411
BstMBI GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
BstNI CCWGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
BstNSI RCATGY 6 cut(s) 913, 1168, 1651, 1678, 1697, 2279
BstSCI CCNGG 6 cut(s) 922, 2407, 2983, 3369, 3561, 3585
BstSFI CTRYAG 1 cut(s) 3347
BstSLI GKGCMC 4 cut(s) 2002, 2440, 3465, 3808
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 8 cut(s) 25, 358, 1972, 2035, 2289, 2469, 3153, 3333
BstV2I GAAGAC 6 cut(s) 282, 751, 797, 1080, 1767, 3830
BstX2I RGATCY 2 cut(s) 1855, 2874
BstXI CCANNNNNNTGG 2 cut(s) 31, 3473
BstYI RGATCY 2 cut(s) 1855, 2874
BsuI GTATCC 3 cut(s) 2458, 2851, 3081
BtgI CCRYGG 4 cut(s) 30, 168, 1782, 3452
BtgZI GCGATG 1 cut(s) 2454
BtrI CACGTC 1 cut(s) 2069
BtsI GCAGTG 1 cut(s) 2910
BtsIMutI CAGTG 7 cut(s) 219, 258, 1292, 1436, 2731, 2910, 3052
BveI ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
Cac8I GCNNGC 8 cut(s) 282, 1191, 1389, 1649, 2208, 2277, 2990, 3684
CaiI CAGNNNCTG 4 cut(s) 1640, 1666, 2035, 3341
CciI TCATGA 1 cut(s) 1333
CfoI GCGC 4 cut(s) 213, 502, 2121, 3595
Cfr10I RCCGGY 1 cut(s) 2717
Cfr13I GGNCC 3 cut(s) 919, 1876, 2063
Csp6I GTAC 2 cut(s) 603, 3010
CspAI ACCGGT 1 cut(s) 2717
CspCI CAANNNNNGTGG 2 cut(s) 2055, 2090
CviQI GTAC 2 cut(s) 603, 3010
DinI GGCGCC 1 cut(s) 212
DpnI GATC 7 cut(s) 696, 739, 1315, 1716, 1857, 2394, 2876
DpnII GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
DraI TTTAAA 2 cut(s) 2530, 3109
DraIII CACNNNGTG 3 cut(s) 1144, 2136, 3047
DriI GACNNNNNGTC 1 cut(s) 1084
EaeI YGGCCR 1 cut(s) 1976
Eam1104I CTCTTC 2 cut(s) 1220, 1261
Eam1105I GACNNNNNGTC 1 cut(s) 1084
EarI CTCTTC 2 cut(s) 1220, 1261
EciI GGCGGA 1 cut(s) 392
Ecl136II GAGCTC 2 cut(s) 280, 3291
Eco130I CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3452
Eco147I AGGCCT 2 cut(s) 896, 1231
Eco24I GRGCYC 3 cut(s) 282, 3293, 3569
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 2548
Eco47I GGWCC 1 cut(s) 1876
Eco47III AGCGCT 1 cut(s) 3594
Eco53kI GAGCTC 2 cut(s) 280, 3291
Eco88I CYCGRG 1 cut(s) 3024
EcoICRI GAGCTC 2 cut(s) 280, 3291
EcoNI CCTNNNNNAGG 1 cut(s) 1225
EcoO109I RGGNCCY 1 cut(s) 919
EcoRI GAATTC 1 cut(s) 794
EcoRII CCWGG 6 cut(s) 922, 2407, 2983, 3369, 3561, 3585
EcoRV GATATC 1 cut(s) 2548
EcoT14I CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3452
EcoT22I ATGCAT 2 cut(s) 976, 2776
EcoT38I GRGCYC 3 cut(s) 282, 3293, 3569
EgeI GGCGCC 1 cut(s) 212
EheI GGCGCC 1 cut(s) 212
ErhI CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3452
Esp3I CGTCTC 1 cut(s) 2158
FaqI GGGAC 2 cut(s) 1031, 3544
FauI CCCGC 1 cut(s) 293
FblI GTMKAC 1 cut(s) 867
FriOI GRGCYC 3 cut(s) 282, 3293, 3569
GlaI GCGC 4 cut(s) 212, 501, 2120, 3594
GsaI CCCAGC 2 cut(s) 615, 2904
GsuI CTGGAG 2 cut(s) 2430, 3641
HaeII RGCGCY 2 cut(s) 214, 3596
HapII CCGG 2 cut(s) 311, 2718
HhaI GCGC 4 cut(s) 213, 502, 2121, 3595
Hin1I GRCGYC 1 cut(s) 211
Hin6I GCGC 4 cut(s) 211, 500, 2119, 3593
HinP1I GCGC 4 cut(s) 211, 500, 2119, 3593
HindIII AAGCTT 4 cut(s) 123, 2376, 2805, 2939
HpaII CCGG 2 cut(s) 311, 2718
Hpy166II GTNNAC 6 cut(s) 868, 1138, 1162, 1920, 2438, 3010
Hpy8I GTNNAC 6 cut(s) 868, 1138, 1162, 1920, 2438, 3010
Hpy99I CGWCG 1 cut(s) 298
HpyCH4III ACNGT 9 cut(s) 253, 1068, 1606, 2037, 2490, 2726, 3243, 3675, 3829
HpyCH4IV ACGT 1 cut(s) 2068
HpySE526I ACGT 1 cut(s) 2068
Hsp92I GRCGYC 1 cut(s) 211
HspAI GCGC 4 cut(s) 211, 500, 2119, 3593
KasI GGCGCC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 310
Kzo9I GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
LguI GCTCTTC 1 cut(s) 1261
LmnI GCTCC 8 cut(s) 26, 277, 725, 844, 947, 2322, 2503, 3296
Lsp1109I GCAGC 8 cut(s) 25, 358, 1972, 2035, 2289, 2469, 3153, 3333
MaeII ACGT 1 cut(s) 2068
MaeIII GTNAC 5 cut(s) 1942, 2031, 2627, 2720, 2906
MalI GATC 7 cut(s) 696, 739, 1315, 1716, 1857, 2394, 2876
MbiI CCGCTC 1 cut(s) 300
MboI GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
MfeI CAATTG 3 cut(s) 263, 2514, 3647
MflI RGATCY 2 cut(s) 1855, 2874
MhlI GDGCHC 7 cut(s) 282, 2002, 2440, 3293, 3465, 3569, 3808
MlsI TGGCCA 1 cut(s) 1978
MluNI TGGCCA 1 cut(s) 1978
Mly113I GGCGCC 1 cut(s) 211
MlyI GAGTC 5 cut(s) 522, 1729, 2738, 2750, 3347
MmeI TCCRAC 2 cut(s) 940, 1756
Mox20I TGGCCA 1 cut(s) 1978
Mph1103I ATGCAT 2 cut(s) 976, 2776
MroI TCCGGA 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 1537, 2664
MscI TGGCCA 1 cut(s) 1978
MslI CAYNNNNRTG 7 cut(s) 29, 324, 620, 1569, 1972, 2616, 3838
Msp20I TGGCCA 1 cut(s) 1978
MspA1I CMGCKG 1 cut(s) 2302
MspCI CTTAAG 1 cut(s) 3734
MspI CCGG 2 cut(s) 311, 2718
MspR9I CCNGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
MunI CAATTG 3 cut(s) 263, 2514, 3647
MvaI CCWGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
MvnI CGCG 1 cut(s) 537
NarI GGCGCC 1 cut(s) 211
NcoI CCATGG 4 cut(s) 30, 168, 1782, 3452
NdeII GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 3 cut(s) 2627, 2720, 2906
NsiI ATGCAT 2 cut(s) 976, 2776
NspI RCATGY 6 cut(s) 913, 1168, 1651, 1678, 1697, 2279
NspV TTCGAA 1 cut(s) 2851
OliI CACNNNNGTG 1 cut(s) 3838
PaeI GCATGC 2 cut(s) 1651, 2279
PagI TCATGA 1 cut(s) 1333
PaqCI CACCTGC 1 cut(s) 2449
PasI CCCWGGG 1 cut(s) 3562
PceI AGGCCT 2 cut(s) 896, 1231
PciI ACATGT 1 cut(s) 1674
PciSI GCTCTTC 1 cut(s) 1261
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PdmI GAANNNNTTC 2 cut(s) 1537, 2664
PflFI GACNNNGTC 1 cut(s) 3745
PflMI CCANNNNNTGG 1 cut(s) 3300
PinAI ACCGGT 1 cut(s) 2717
PleI GAGTC 5 cut(s) 522, 1728, 2738, 2750, 3346
PluTI GGCGCC 1 cut(s) 214
PpsI GAGTC 5 cut(s) 522, 1728, 2738, 2750, 3346
PscI ACATGT 1 cut(s) 1674
Psp124BI GAGCTC 2 cut(s) 282, 3293
Psp6I CCWGG 6 cut(s) 922, 2407, 2983, 3369, 3561, 3585
PspFI CCCAGC 2 cut(s) 611, 2900
PspGI CCWGG 6 cut(s) 922, 2407, 2983, 3369, 3561, 3585
PspPI GGNCC 3 cut(s) 919, 1876, 2063
PstI CTGCAG 1 cut(s) 3351
PstNI CAGNNNCTG 4 cut(s) 1640, 1666, 2035, 3341
PsuI RGATCY 2 cut(s) 1855, 2874
PsyI GACNNNGTC 1 cut(s) 3745
PvuII CAGCTG 1 cut(s) 2302
RsaI GTAC 2 cut(s) 604, 3011
RsaNI GTAC 2 cut(s) 603, 3010
RseI CAYNNNNRTG 7 cut(s) 29, 324, 620, 1569, 1972, 2616, 3838
SacI GAGCTC 2 cut(s) 282, 3293
SapI GCTCTTC 1 cut(s) 1261
Sau3AI GATC 7 cut(s) 694, 737, 1313, 1714, 1855, 2392, 2874
Sau96I GGNCC 3 cut(s) 919, 1876, 2063
SchI GAGTC 5 cut(s) 522, 1729, 2738, 2750, 3347
ScrFI CCNGG 6 cut(s) 924, 2409, 2985, 3371, 3563, 3587
SduI GDGCHC 7 cut(s) 282, 2002, 2440, 3293, 3465, 3569, 3808
SfcI CTRYAG 1 cut(s) 3347
SfoI GGCGCC 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 2851
SinI GGWCC 1 cut(s) 1876
SmiI ATTTAAAT 1 cut(s) 2530
SmiMI CAYNNNNRTG 7 cut(s) 29, 324, 620, 1569, 1972, 2616, 3838
SmlI CTYRAG 4 cut(s) 428, 508, 1993, 3734
SmoI CTYRAG 4 cut(s) 428, 508, 1993, 3734
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 2 cut(s) 1651, 2279
SseBI AGGCCT 2 cut(s) 896, 1231
SspDI GGCGCC 1 cut(s) 210
SspI AATATT 5 cut(s) 2534, 3105, 3438, 3552, 3604
SstI GAGCTC 2 cut(s) 282, 3293
StuI AGGCCT 2 cut(s) 896, 1231
StyD4I CCNGG 6 cut(s) 922, 2407, 2983, 3369, 3561, 3585
StyI CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3452
SwaI ATTTAAAT 1 cut(s) 2530
TaaI ACNGT 9 cut(s) 253, 1068, 1606, 2037, 2490, 2726, 3243, 3675, 3829
TaiI ACGT 1 cut(s) 2071
TaqI TCGA 6 cut(s) 89, 293, 2019, 2323, 2851, 3677
TatI WGTACW 2 cut(s) 602, 3009
TauI GCSGC 5 cut(s) 44, 69, 149, 377, 2995
TscAI CASTG 7 cut(s) 226, 258, 1292, 1436, 2731, 2917, 3052
TseFI GTSAC 3 cut(s) 2627, 2720, 2906
TseI GCWGC 8 cut(s) 38, 371, 1985, 2023, 2302, 2482, 3166, 3346
Tsp45I GTSAC 3 cut(s) 2627, 2720, 2906
TspGWI ACGGA 4 cut(s) 1762, 2140, 2567, 3709
TspRI CASTG 7 cut(s) 226, 258, 1292, 1436, 2731, 2917, 3052
Tth111I GACNNNGTC 1 cut(s) 3745
Van91I CCANNNNNTGG 1 cut(s) 3300
Vha464I CTTAAG 1 cut(s) 3734
VneI GTGCAC 1 cut(s) 2436
VpaK11BI GGWCC 1 cut(s) 1876
XagI CCTNNNNNAGG 1 cut(s) 1225
XceI RCATGY 6 cut(s) 913, 1168, 1651, 1678, 1697, 2279
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 2 cut(s) 1537, 2664
Zsp2I ATGCAT 2 cut(s) 976, 2776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.