DNA polymerase
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G64420 |
| fragaria_vesca | FvH4_2g11160 |
| malus_domestica | MD05G1049800.v1.1 MD10G1056900.v1.1 |
| prunus_persica | Prupe.8G075100_v2.0.a1 |
| pyrus_communis | pycom05g04160 pycom05g04170 pycom10g04380 |
| rosa_chinensis | RchiOBHm_Chr6g0269481 |
| rosa_laevigata | RLG00000013848 RLG00000013849 |
| rosa_multiflora | Rmu_ssc0000291.1_g000033 |
| rosa_roxburghii | Rroxscaffold_7G00198700 |
| rosa_rugosa | Rorug06G0048500 |
| rosa_samantha | Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100 |
| rosa_wichuraiana | Rw6G014500 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2191 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2479 |
| Acc36I | ACCTGC | 4 cut(s) | 2191, 2254, 2353, 2727 |
| AccII | CGCG | 1 cut(s) | 2775 |
| AccIII | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| AclWI | GGATC | 3 cut(s) | 1053, 1066, 2630 |
| AcoI | YGGCCR | 1 cut(s) | 1718 |
| AcuI | CTGAAG | 6 cut(s) | 960, 1357, 1440, 1701, 3077, 3456 |
| AcvI | CACGTG | 1 cut(s) | 1811 |
| AcyI | GRCGYC | 2 cut(s) | 90, 160 |
| AdeI | CACNNNGTG | 2 cut(s) | 1878, 3713 |
| AfaI | GTAC | 3 cut(s) | 577, 1191, 3441 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 2875, 3206 |
| AflII | CTTAAG | 1 cut(s) | 3482 |
| AflIII | ACRYGT | 1 cut(s) | 1419 |
| AhlI | ACTAGT | 1 cut(s) | 773 |
| AjnI | CCWGG | 3 cut(s) | 2999, 3117, 3333 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 395, 427, 3184, 3216, 3531, 3563 |
| AleI | CACNNNNGTG | 1 cut(s) | 1682 |
| Alw21I | GWGCWC | 3 cut(s) | 271, 703, 1744 |
| Alw26I | GTCTC | 7 cut(s) | 118, 151, 493, 2435, 3159, 3421, 3675 |
| AlwI | GGATC | 3 cut(s) | 1053, 1066, 2630 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2093 |
| Ama87I | CYCGRG | 2 cut(s) | 3310, 3728 |
| Aor13HI | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| AoxI | GGCC | 8 cut(s) | 441, 893, 1095, 1718, 2487, 2994, 3197, 3582 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 655, 687, 3364, 3396 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 55, 1282, 2075, 2400 |
| AspLEI | GCGC | 3 cut(s) | 175, 475, 2777 |
| AspS9I | GGNCC | 2 cut(s) | 1805, 2866 |
| AsuC2I | CCSGG | 2 cut(s) | 3311, 3312 |
| AvaI | CYCGRG | 2 cut(s) | 3310, 3728 |
| AvaII | GGWCC | 2 cut(s) | 1805, 2866 |
| BalI | TGGCCA | 1 cut(s) | 1720 |
| BamHI | GGATCC | 1 cut(s) | 1058 |
| BanII | GRGCYC | 1 cut(s) | 271 |
| BauI | CACGAG | 1 cut(s) | 3672 |
| BbrPI | CACGTG | 1 cut(s) | 1811 |
| BbsI | GAAGAC | 7 cut(s) | 246, 306, 724, 1512, 2163, 2260, 2379 |
| Bbv12I | GWGCWC | 3 cut(s) | 271, 703, 1744 |
| BbvCI | CCTCAGC | 1 cut(s) | 726 |
| BceAI | ACGGC | 1 cut(s) | 460 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 3653, 3687 |
| BciT130I | CCWGG | 3 cut(s) | 3001, 3119, 3335 |
| BciVI | GTATCC | 3 cut(s) | 1909, 2200, 2599 |
| BclI | TGATCA | 2 cut(s) | 1047, 3553 |
| BcnI | CCSGG | 2 cut(s) | 3311, 3312 |
| BcoDI | GTCTC | 7 cut(s) | 118, 151, 493, 2435, 3159, 3421, 3675 |
| BcuI | ACTAGT | 1 cut(s) | 773 |
| BfaI | CTAG | 9 cut(s) | 258, 722, 774, 902, 1595, 1643, 2349, 2708, 2903 |
| BfmI | CTRYAG | 4 cut(s) | 1301, 2184, 2796, 3095 |
| BfrI | CTTAAG | 1 cut(s) | 3482 |
| BfuAI | ACCTGC | 4 cut(s) | 2191, 2254, 2353, 2727 |
| BfuI | GTATCC | 3 cut(s) | 1909, 2200, 2599 |
| BglII | AGATCT | 1 cut(s) | 3459 |
| BlpI | GCTNAGC | 2 cut(s) | 1994, 3488 |
| Bme1390I | CCNGG | 5 cut(s) | 3001, 3119, 3311, 3312, 3335 |
| Bme18I | GGWCC | 2 cut(s) | 1805, 2866 |
| BmeT110I | CYCGRG | 2 cut(s) | 3310, 3728 |
| BmgT120I | GGNCC | 2 cut(s) | 1805, 2866 |
| BmiI | GGNNCC | 3 cut(s) | 1060, 3005, 3679 |
| BmrFI | CCNGG | 5 cut(s) | 3001, 3119, 3311, 3312, 3335 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1831 |
| BpiI | GAAGAC | 7 cut(s) | 246, 306, 724, 1512, 2163, 2260, 2379 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 475, 507, 2477, 2509, 2489, 2521 |
| BpmI | CTGGAG | 3 cut(s) | 1754, 3501, 3720 |
| Bpu10I | CCTNAGC | 1 cut(s) | 726 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1994, 3488 |
| BpuEI | CTTGAG | 4 cut(s) | 1199, 1720, 1850, 3488 |
| BpuMI | CCSGG | 2 cut(s) | 3311, 3312 |
| BsaAI | YACGTR | 1 cut(s) | 1811 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2610 |
| BsaHI | GRCGYC | 2 cut(s) | 90, 160 |
| BsaWI | WCCGGW | 4 cut(s) | 280, 495, 1600, 2598 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 2875, 3206 |
| Bse118I | RCCGGY | 1 cut(s) | 239 |
| Bse1I | ACTGG | 4 cut(s) | 1030, 1174, 1771, 2449 |
| Bse3DI | GCAATG | 8 cut(s) | 602, 783, 1321, 1530, 1959, 2210, 2219, 2598 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2610 |
| BseAI | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| BseBI | CCWGG | 3 cut(s) | 3001, 3119, 3335 |
| BseJI | GATNNNNATC | 1 cut(s) | 2610 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 2875, 3206 |
| BseMI | GCAATG | 8 cut(s) | 602, 783, 1321, 1530, 1959, 2210, 2219, 2598 |
| BseMII | CTCAG | 9 cut(s) | 717, 1479, 1805, 2085, 2430, 2475, 2487, 2499, 3479 |
| BseNI | ACTGG | 4 cut(s) | 1030, 1174, 1771, 2449 |
| BseRI | GAGGAG | 1 cut(s) | 743 |
| BseYI | CCCAGC | 1 cut(s) | 2648 |
| BsgI | GTGCAG | 3 cut(s) | 398, 740, 1947 |
| Bsh1236I | CGCG | 1 cut(s) | 2775 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2069 |
| BshFI | GGCC | 8 cut(s) | 443, 895, 1097, 1720, 2489, 2996, 3199, 3584 |
| BsiEI | CGRYCG | 1 cut(s) | 2069 |
| BsiHKAI | GWGCWC | 3 cut(s) | 271, 703, 1744 |
| BsiHKCI | CYCGRG | 2 cut(s) | 3310, 3728 |
| BsiSI | CCGG | 7 cut(s) | 240, 281, 496, 1601, 2599, 3311, 3681 |
| BslFI | GGGAC | 1 cut(s) | 3292 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 2875, 3206 |
| BsmAI | GTCTC | 7 cut(s) | 118, 151, 493, 2435, 3159, 3421, 3675 |
| BsmBI | CGTCTC | 2 cut(s) | 151, 3421 |
| BsmFI | GGGAC | 1 cut(s) | 3292 |
| BsmI | GAATGC | 2 cut(s) | 22, 879 |
| BsnI | GGCC | 8 cut(s) | 443, 895, 1097, 1720, 2489, 2996, 3199, 3584 |
| BsoBI | CYCGRG | 2 cut(s) | 3310, 3728 |
| Bsp1286I | GDGCHC | 3 cut(s) | 271, 703, 1744 |
| Bsp13I | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1994, 3488 |
| Bsp19I | CCATGG | 3 cut(s) | 117, 192, 2538 |
| BspANI | GGCC | 8 cut(s) | 443, 895, 1097, 1720, 2489, 2996, 3199, 3584 |
| BspCNI | CTCAG | 9 cut(s) | 718, 1480, 1804, 2086, 2431, 2476, 2488, 2500, 3480 |
| BspEI | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| BspFNI | CGCG | 1 cut(s) | 2775 |
| BspLI | GGNNCC | 3 cut(s) | 1060, 3005, 3679 |
| BspMAI | CTGCAG | 3 cut(s) | 2188, 2800, 3099 |
| BspMI | ACCTGC | 4 cut(s) | 2191, 2254, 2353, 2727 |
| BspPI | GGATC | 3 cut(s) | 1053, 1066, 2630 |
| BspQI | GCTCTTC | 2 cut(s) | 276, 1006 |
| BspTI | CTTAAG | 1 cut(s) | 3482 |
| BsrDI | GCAATG | 8 cut(s) | 602, 783, 1321, 1530, 1959, 2210, 2219, 2598 |
| BsrFI | RCCGGY | 1 cut(s) | 239 |
| BsrI | ACTGG | 4 cut(s) | 1030, 1174, 1771, 2449 |
| BssAI | RCCGGY | 1 cut(s) | 239 |
| BssNI | GRCGYC | 2 cut(s) | 90, 160 |
| BssSI | CACGAG | 1 cut(s) | 3672 |
| BssT1I | CCWWGG | 6 cut(s) | 117, 192, 1920, 2538, 2874, 3200 |
| Bst2BI | CACGAG | 1 cut(s) | 3672 |
| Bst2UI | CCWGG | 3 cut(s) | 3001, 3119, 3335 |
| Bst4CI | ACNGT | 5 cut(s) | 109, 470, 1351, 2232, 2462 |
| Bst6I | CTCTTC | 4 cut(s) | 276, 797, 1006, 3738 |
| BstACI | GRCGYC | 2 cut(s) | 90, 160 |
| BstAFI | CTTAAG | 1 cut(s) | 3482 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1878, 2677 |
| BstBAI | YACGTR | 1 cut(s) | 1811 |
| BstDSI | CCRYGG | 3 cut(s) | 117, 192, 2538 |
| BstEII | GGTNACC | 1 cut(s) | 3716 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3204 |
| BstFNI | CGCG | 1 cut(s) | 2775 |
| BstHHI | GCGC | 3 cut(s) | 175, 475, 2777 |
| BstMAI | GTCTC | 7 cut(s) | 118, 151, 493, 2435, 3159, 3421, 3675 |
| BstMCI | CGRYCG | 1 cut(s) | 2069 |
| BstNI | CCWGG | 3 cut(s) | 3001, 3119, 3335 |
| BstNSI | RCATGY | 3 cut(s) | 1396, 1423, 2021 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1831 |
| BstPI | GGTNACC | 1 cut(s) | 3716 |
| BstSCI | CCNGG | 5 cut(s) | 2999, 3117, 3309, 3310, 3333 |
| BstSFI | CTRYAG | 4 cut(s) | 1301, 2184, 2796, 3095 |
| BstUI | CGCG | 1 cut(s) | 2775 |
| BstV2I | GAAGAC | 7 cut(s) | 246, 306, 724, 1512, 2163, 2260, 2379 |
| BstX2I | RGATCY | 3 cut(s) | 1058, 2622, 3459 |
| BstYI | RGATCY | 3 cut(s) | 1058, 2622, 3459 |
| BsuI | GTATCC | 3 cut(s) | 1909, 2200, 2599 |
| BsuRI | GGCC | 8 cut(s) | 443, 895, 1097, 1720, 2489, 2996, 3199, 3584 |
| BtgI | CCRYGG | 3 cut(s) | 117, 192, 2538 |
| BtgZI | GCGATG | 1 cut(s) | 2187 |
| BtsI | GCAGTG | 5 cut(s) | 1662, 2005, 2094, 2658, 2812 |
| BveI | ACCTGC | 4 cut(s) | 2191, 2254, 2353, 2727 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2093 |
| CfoI | GCGC | 3 cut(s) | 175, 475, 2777 |
| Cfr10I | RCCGGY | 1 cut(s) | 239 |
| Cfr13I | GGNCC | 2 cut(s) | 1805, 2866 |
| Cfr9I | CCCGGG | 1 cut(s) | 3310 |
| CseI | GACGC | 5 cut(s) | 98, 168, 240, 2186, 2561 |
| Csp6I | GTAC | 3 cut(s) | 576, 1190, 3440 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1797, 1832, 2730, 2765 |
| CviQI | GTAC | 3 cut(s) | 576, 1190, 3440 |
| DraI | TTTAAA | 2 cut(s) | 1590, 2857 |
| DraIII | CACNNNGTG | 2 cut(s) | 1878, 3713 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2479 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2479 |
| EaeI | YGGCCR | 1 cut(s) | 1718 |
| Eam1104I | CTCTTC | 4 cut(s) | 276, 797, 1006, 3738 |
| EarI | CTCTTC | 4 cut(s) | 276, 797, 1006, 3738 |
| EciI | GGCGGA | 3 cut(s) | 258, 279, 362 |
| Ecl136II | GAGCTC | 1 cut(s) | 269 |
| Eco130I | CCWWGG | 6 cut(s) | 117, 192, 1920, 2538, 2874, 3200 |
| Eco147I | AGGCCT | 1 cut(s) | 3199 |
| Eco24I | GRGCYC | 1 cut(s) | 271 |
| Eco47I | GGWCC | 2 cut(s) | 1805, 2866 |
| Eco53kI | GAGCTC | 1 cut(s) | 269 |
| Eco57I | CTGAAG | 6 cut(s) | 960, 1357, 1440, 1701, 3077, 3456 |
| Eco72I | CACGTG | 1 cut(s) | 1811 |
| Eco88I | CYCGRG | 2 cut(s) | 3310, 3728 |
| Eco91I | GGTNACC | 1 cut(s) | 3716 |
| EcoICRI | GAGCTC | 1 cut(s) | 269 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3204 |
| EcoO65I | GGTNACC | 1 cut(s) | 3716 |
| EcoRI | GAATTC | 3 cut(s) | 767, 2075, 2835 |
| EcoRII | CCWGG | 3 cut(s) | 2999, 3117, 3333 |
| EcoT14I | CCWWGG | 6 cut(s) | 117, 192, 1920, 2538, 2874, 3200 |
| EcoT38I | GRGCYC | 1 cut(s) | 271 |
| ErhI | CCWWGG | 6 cut(s) | 117, 192, 1920, 2538, 2874, 3200 |
| Esp3I | CGTCTC | 2 cut(s) | 151, 3421 |
| FaqI | GGGAC | 1 cut(s) | 3292 |
| FauI | CCCGC | 2 cut(s) | 238, 3446 |
| FauNDI | CATATG | 1 cut(s) | 3666 |
| FbaI | TGATCA | 2 cut(s) | 1047, 3553 |
| FriOI | GRGCYC | 1 cut(s) | 271 |
| FspBI | CTAG | 9 cut(s) | 258, 722, 774, 902, 1595, 1643, 2349, 2708, 2903 |
| GlaI | GCGC | 3 cut(s) | 174, 474, 2776 |
| GsaI | CCCAGC | 1 cut(s) | 2652 |
| GsuI | CTGGAG | 3 cut(s) | 1754, 3501, 3720 |
| HaeIII | GGCC | 8 cut(s) | 443, 895, 1097, 1720, 2489, 2996, 3199, 3584 |
| HapII | CCGG | 7 cut(s) | 240, 281, 496, 1601, 2599, 3311, 3681 |
| HgaI | GACGC | 5 cut(s) | 98, 168, 240, 2186, 2561 |
| HhaI | GCGC | 3 cut(s) | 175, 475, 2777 |
| Hin1I | GRCGYC | 2 cut(s) | 90, 160 |
| Hin6I | GCGC | 3 cut(s) | 173, 473, 2775 |
| HinP1I | GCGC | 3 cut(s) | 173, 473, 2775 |
| HindIII | AAGCTT | 3 cut(s) | 54, 2687, 3339 |
| HpaII | CCGG | 7 cut(s) | 240, 281, 496, 1601, 2599, 3311, 3681 |
| Hpy166II | GTNNAC | 6 cut(s) | 598, 1662, 1808, 2758, 2810, 3707 |
| Hpy8I | GTNNAC | 6 cut(s) | 598, 1662, 1808, 2758, 2810, 3707 |
| Hpy99I | CGWCG | 3 cut(s) | 364, 481, 2384 |
| HpyCH4III | ACNGT | 5 cut(s) | 109, 470, 1351, 2232, 2462 |
| HpyCH4IV | ACGT | 1 cut(s) | 1810 |
| HpySE526I | ACGT | 1 cut(s) | 1810 |
| Hsp92I | GRCGYC | 2 cut(s) | 90, 160 |
| HspAI | GCGC | 3 cut(s) | 173, 473, 2775 |
| Kpn2I | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| KroI | GCCGGC | 1 cut(s) | 239 |
| KroNI | GCCGGC | 1 cut(s) | 241 |
| Ksp22I | TGATCA | 2 cut(s) | 1047, 3553 |
| LguI | GCTCTTC | 2 cut(s) | 276, 1006 |
| LmnI | GCTCC | 7 cut(s) | 245, 266, 364, 1773, 2064, 2245, 3730 |
| MaeI | CTAG | 9 cut(s) | 258, 722, 774, 902, 1595, 1643, 2349, 2708, 2903 |
| MaeII | ACGT | 1 cut(s) | 1810 |
| MaeIII | GTNAC | 6 cut(s) | 634, 1684, 2456, 2654, 3686, 3716 |
| MfeI | CAATTG | 1 cut(s) | 3395 |
| MflI | RGATCY | 3 cut(s) | 1058, 2622, 3459 |
| MhlI | GDGCHC | 3 cut(s) | 271, 703, 1744 |
| MlsI | TGGCCA | 1 cut(s) | 1720 |
| MluNI | TGGCCA | 1 cut(s) | 1720 |
| MmeI | TCCRAC | 2 cut(s) | 417, 1220 |
| Mox20I | TGGCCA | 1 cut(s) | 1720 |
| MroI | TCCGGA | 3 cut(s) | 280, 495, 2598 |
| MroNI | GCCGGC | 1 cut(s) | 239 |
| MroXI | GAANNNNTTC | 4 cut(s) | 55, 1282, 2075, 2400 |
| MscI | TGGCCA | 1 cut(s) | 1720 |
| MslI | CAYNNNNRTG | 4 cut(s) | 593, 1424, 1682, 2358 |
| Msp20I | TGGCCA | 1 cut(s) | 1720 |
| MspA1I | CMGCKG | 2 cut(s) | 236, 2044 |
| MspCI | CTTAAG | 1 cut(s) | 3482 |
| MspI | CCGG | 7 cut(s) | 240, 281, 496, 1601, 2599, 3311, 3681 |
| MspR9I | CCNGG | 5 cut(s) | 3001, 3119, 3311, 3312, 3335 |
| MunI | CAATTG | 1 cut(s) | 3395 |
| Mva1269I | GAATGC | 2 cut(s) | 22, 879 |
| MvaI | CCWGG | 3 cut(s) | 3001, 3119, 3335 |
| MvnI | CGCG | 1 cut(s) | 2775 |
| NaeI | GCCGGC | 1 cut(s) | 241 |
| NciI | CCSGG | 2 cut(s) | 3311, 3312 |
| NcoI | CCATGG | 3 cut(s) | 117, 192, 2538 |
| NdeI | CATATG | 1 cut(s) | 3666 |
| NgoMIV | GCCGGC | 1 cut(s) | 239 |
| NlaIV | GGNNCC | 3 cut(s) | 1060, 3005, 3679 |
| NmeAIII | GCCGAG | 1 cut(s) | 469 |
| NmuCI | GTSAC | 6 cut(s) | 634, 1684, 2456, 2654, 3686, 3716 |
| NspI | RCATGY | 3 cut(s) | 1396, 1423, 2021 |
| OliI | CACNNNNGTG | 1 cut(s) | 1682 |
| PaeI | GCATGC | 2 cut(s) | 1396, 2021 |
| PaqCI | CACCTGC | 1 cut(s) | 2191 |
| PceI | AGGCCT | 1 cut(s) | 3199 |
| PciI | ACATGT | 1 cut(s) | 1419 |
| PciSI | GCTCTTC | 2 cut(s) | 276, 1006 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2385 |
| PctI | GAATGC | 2 cut(s) | 22, 879 |
| PdiI | GCCGGC | 1 cut(s) | 241 |
| PdmI | GAANNNNTTC | 4 cut(s) | 55, 1282, 2075, 2400 |
| PfoI | TCCNGGA | 1 cut(s) | 2999 |
| PmaCI | CACGTG | 1 cut(s) | 1811 |
| PmlI | CACGTG | 1 cut(s) | 1811 |
| Ppu21I | YACGTR | 1 cut(s) | 1811 |
| PscI | ACATGT | 1 cut(s) | 1419 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1831 |
| Psp124BI | GAGCTC | 1 cut(s) | 271 |
| Psp6I | CCWGG | 3 cut(s) | 2999, 3117, 3333 |
| PspCI | CACGTG | 1 cut(s) | 1811 |
| PspEI | GGTNACC | 1 cut(s) | 3716 |
| PspFI | CCCAGC | 1 cut(s) | 2648 |
| PspGI | CCWGG | 3 cut(s) | 2999, 3117, 3333 |
| PspN4I | GGNNCC | 3 cut(s) | 1060, 3005, 3679 |
| PspPI | GGNCC | 2 cut(s) | 1805, 2866 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2181, 2213 |
| PstI | CTGCAG | 3 cut(s) | 2188, 2800, 3099 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2093 |
| PsuI | RGATCY | 3 cut(s) | 1058, 2622, 3459 |
| PvuII | CAGCTG | 1 cut(s) | 2044 |
| RsaI | GTAC | 3 cut(s) | 577, 1191, 3441 |
| RsaNI | GTAC | 3 cut(s) | 576, 1190, 3440 |
| RseI | CAYNNNNRTG | 4 cut(s) | 593, 1424, 1682, 2358 |
| SacI | GAGCTC | 1 cut(s) | 271 |
| SapI | GCTCTTC | 2 cut(s) | 276, 1006 |
| Sau96I | GGNCC | 2 cut(s) | 1805, 2866 |
| ScrFI | CCNGG | 5 cut(s) | 3001, 3119, 3311, 3312, 3335 |
| SduI | GDGCHC | 3 cut(s) | 271, 703, 1744 |
| SfcI | CTRYAG | 4 cut(s) | 1301, 2184, 2796, 3095 |
| SinI | GGWCC | 2 cut(s) | 1805, 2866 |
| SmaI | CCCGGG | 1 cut(s) | 3312 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 593, 1424, 1682, 2358 |
| SmlI | CTYRAG | 5 cut(s) | 1214, 1735, 1829, 3482, 3503 |
| SmoI | CTYRAG | 5 cut(s) | 1214, 1735, 1829, 3482, 3503 |
| SpeI | ACTAGT | 1 cut(s) | 773 |
| SphI | GCATGC | 2 cut(s) | 1396, 2021 |
| SseBI | AGGCCT | 1 cut(s) | 3199 |
| SspI | AATATT | 5 cut(s) | 910, 2853, 3137, 3186, 3300 |
| SspMI | CTAG | 9 cut(s) | 258, 722, 774, 902, 1595, 1643, 2349, 2708, 2903 |
| SstI | GAGCTC | 1 cut(s) | 271 |
| StuI | AGGCCT | 1 cut(s) | 3199 |
| StyD4I | CCNGG | 5 cut(s) | 2999, 3117, 3309, 3310, 3333 |
| StyI | CCWWGG | 6 cut(s) | 117, 192, 1920, 2538, 2874, 3200 |
| TaaI | ACNGT | 5 cut(s) | 109, 470, 1351, 2232, 2462 |
| TaiI | ACGT | 1 cut(s) | 1813 |
| TaqI | TCGA | 9 cut(s) | 14, 524, 1825, 1906, 2065, 2379, 3390, 3425, 3650 |
| TaqII | GACCGA | 1 cut(s) | 2083 |
| TatI | WGTACW | 1 cut(s) | 1189 |
| TauI | GCSGC | 6 cut(s) | 229, 236, 264, 329, 344, 347 |
| TseFI | GTSAC | 6 cut(s) | 634, 1684, 2456, 2654, 3686, 3716 |
| Tsp45I | GTSAC | 6 cut(s) | 634, 1684, 2456, 2654, 3686, 3716 |
| TspGWI | ACGGA | 4 cut(s) | 377, 2309, 2635, 3457 |
| TspMI | CCCGGG | 1 cut(s) | 3310 |
| Vha464I | CTTAAG | 1 cut(s) | 3482 |
| VpaK11BI | GGWCC | 2 cut(s) | 1805, 2866 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3204 |
| XbaI | TCTAGA | 1 cut(s) | 2902 |
| XceI | RCATGY | 3 cut(s) | 1396, 1423, 2021 |
| XmaI | CCCGGG | 1 cut(s) | 3310 |
| XmnI | GAANNNNTTC | 4 cut(s) | 55, 1282, 2075, 2400 |
| XspI | CTAG | 9 cut(s) | 258, 722, 774, 902, 1595, 1643, 2349, 2708, 2903 |