AT5G65205

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013333
Physical Location & Seq
Reverse (-)
26050790 .. 26052207
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65205.1

Sequence Viewer

Length: 858 bp
ATGGGAAGCAGCAGCGACGAGACGCCAGTGGTTCTCATCACAGGATGTTCTCAGGGAGGAATCGGTCACGCGTTGGCGCGTGAGTTCTCGGCTAATGGTTGTCGTGTGGTGGCGACGAGTCGATCGCAGAAGACGATGACTGAATTGGAGAAAGATTCCAAGTTCTTTGTGCAAGAGCTCGATGTTCAGTCTGAGCAGAGCGTGAGTAAAGTTGTGTCGAAAGTTATCGACAAATTTGGTCAGATTGATGTTTTGGTTAATAACGCCGGAGTTCAGTGTATCGGACCTTTGGCGGAGATTCCAATTTCAGCCATGGATTACACCTTCAACACCAATGTGCTCGGTTCCATGAGGATGACTCAAGCTGTTGTACCTCACATGGCGTCTAAGAAAAAGGGAAAGATTGTGAACATAGGAAGTATTAGCATAATGGCACCAGGACCATGGGCTGGGGTTTATACTGCATCAAAAGCTGCTCTTCATGCTCTTACCGACACTTTAAGGTTGGAGCTTAAGCCATTTGGGATTGATGTAATCAACATTGTACCAGGAGGTATTCAATCAAACATAGCTAACTCAGGGATATCGAGTTTCAACAATTTACCTGAATTGAAACTATACAAACCGTTCGAGGACGCTATCCGTGAGAGAGCGTTTCTGTCGCAAAACATAAAGCCGATACCCACAGAGACTTTTGCGAAAGAGACTGTTTCCGTGGTGCTGAAGAAAAACCCACCGGCTTGGTACTCTACAGGTAGGTTATCTACAGTCATGGCGATCATGCACCATATACCGATTTTCATGAAAGATTTTCTGTTGACTAAGAGTTTTATGAAAAAGGGAGTAAAACCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

285

Amino Acids

30.92

Weight (kDa)

9.25

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1776 PF08643 7 - 188 7e-09 Fungal family of unknown function (DUF1776)
SDR PF23441 8 - 190 5.8e-06 SDR-like rossmann domain
adh_short PF00106 10 - 192 4.8e-53 short chain dehydrogenase
KR PF08659 11 - 168 2.6e-10 KR domain
Epimerase PF01370 11 - 147 1.1e-07 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 18 - 201 9.2e-37 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10050 AT5G65205
fragaria_vesca FvH4_1g21770 FvH4_1g21770
malus_domestica MD01G1022400.v1.1
prunus_persica Prupe.6G178200_v2.0.a1
pyrus_communis pycom01g05090
rosa_chinensis RchiOBHm_Chr2g0115641
rosa_laevigata RLG00000018200
rosa_multiflora Rmu_ssc0000197.1_g000085
rosa_roxburghii Rroxscaffold_2G00128030 Rroxscaffold_2G00128900
rosa_rugosa Rorug02G0199000
rosa_samantha Rh2AG256100 Rh2BG266900 Rh2CG261500 Rh2DG264400
rosa_wichuraiana Rw2G020050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccB7I CCANNNNNTGG 1 cut(s) 449
AccII CGCG 2 cut(s) 71, 79
AciI CCGC 1 cut(s) 293
AcsI RAATTY 1 cut(s) 233
AcuI CTGAAG 1 cut(s) 743
AcyI GRCGYC 2 cut(s) 23, 383
AfaI GTAC 3 cut(s) 372, 546, 746
AfiI CCNNNNNNNGG 1 cut(s) 449
AflII CTTAAG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 4 cut(s) 328, 560, 595, 613
AjnI CCWGG 2 cut(s) 436, 547
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 5 cut(s) 178, 365, 473, 511, 572
AluI AGCT 5 cut(s) 178, 365, 473, 511, 572
Alw21I GWGCWC 2 cut(s) 180, 342
Alw26I GTCTC 3 cut(s) 14, 683, 698
ApeKI GCWGC 3 cut(s) 9, 12, 473
ApoI RAATTY 1 cut(s) 233
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 2 cut(s) 284, 440
AvaII GGWCC 2 cut(s) 284, 440
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 180, 342
BbvI GCAGC 3 cut(s) 21, 24, 460
BciT130I CCWGG 2 cut(s) 438, 549
BcoDI GTCTC 3 cut(s) 14, 683, 698
BfmI CTRYAG 2 cut(s) 750, 765
BfrI CTTAAG 1 cut(s) 512
BisI GCNGC 3 cut(s) 10, 13, 474
BlsI GCNGC 3 cut(s) 11, 14, 475
Bme1390I CCNGG 2 cut(s) 438, 549
Bme18I GGWCC 2 cut(s) 284, 440
BmgT120I GGNCC 2 cut(s) 284, 440
BmiI GGNNCC 2 cut(s) 346, 435
BmrFI CCNGG 2 cut(s) 438, 549
BmsI GCATC 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpuEI CTTGAG 1 cut(s) 345
BsaHI GRCGYC 2 cut(s) 23, 383
BsaJI CCNNGG 3 cut(s) 312, 443, 714
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 1 cut(s) 26
BseBI CCWGG 2 cut(s) 438, 549
BseDI CCNNGG 3 cut(s) 312, 443, 714
BseGI GGATG 2 cut(s) 50, 360
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMII CTCAG 3 cut(s) 65, 183, 591
BseNI ACTGG 1 cut(s) 26
BseXI GCAGC 3 cut(s) 21, 24, 460
BseYI CCCAGC 1 cut(s) 449
Bsh1236I CGCG 2 cut(s) 71, 79
Bsh1285I CGRYCG 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 180, 342
BsiSI CCGG 2 cut(s) 267, 737
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 3 cut(s) 14, 683, 698
BsmBI CGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 180, 342
Bsp143I GATC 2 cut(s) 122, 777
Bsp19I CCATGG 2 cut(s) 312, 443
BspACI CCGC 1 cut(s) 293
BspCNI CTCAG 3 cut(s) 64, 184, 590
BspFNI CGCG 2 cut(s) 71, 79
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 2 cut(s) 346, 435
BspQI GCTCTTC 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 433
BspTI CTTAAG 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 736
BssECI CCNNGG 3 cut(s) 312, 443, 714
BssMI GATC 2 cut(s) 122, 777
BssNI GRCGYC 2 cut(s) 23, 383
BssT1I CCWWGG 2 cut(s) 312, 443
Bst2UI CCWGG 2 cut(s) 438, 549
Bst4CI ACNGT 3 cut(s) 627, 709, 769
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 2 cut(s) 23, 383
BstAFI CTTAAG 1 cut(s) 512
BstDEI CTNAG 5 cut(s) 51, 192, 387, 577, 822
BstDSI CCRYGG 3 cut(s) 312, 443, 714
BstF5I GGATG 2 cut(s) 50, 360
BstFNI CGCG 2 cut(s) 71, 79
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 2 cut(s) 125, 780
BstMAI GTCTC 3 cut(s) 14, 683, 698
BstMBI GATC 2 cut(s) 122, 777
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 2 cut(s) 470, 482
BstNI CCWGG 2 cut(s) 438, 549
BstSCI CCNGG 2 cut(s) 436, 547
BstSFI CTRYAG 2 cut(s) 750, 765
BstUI CGCG 2 cut(s) 71, 79
BstV1I GCAGC 3 cut(s) 21, 24, 460
BstV2I GAAGAC 1 cut(s) 137
BstXI CCANNNNNNTGG 2 cut(s) 444, 741
BtgI CCRYGG 3 cut(s) 312, 443, 714
BtsCI GGATG 2 cut(s) 50, 360
BtsIMutI CAGTG 2 cut(s) 33, 281
CciI TCATGA 1 cut(s) 801
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 736
Cfr13I GGNCC 2 cut(s) 284, 440
CseI GACGC 3 cut(s) 31, 372, 644
Csp6I GTAC 3 cut(s) 371, 545, 745
CviAII CATG 8 cut(s) 313, 349, 379, 444, 482, 772, 781, 802
CviQI GTAC 3 cut(s) 371, 545, 745
DdeI CTNAG 5 cut(s) 51, 192, 387, 577, 822
DpnI GATC 2 cut(s) 124, 779
DpnII GATC 2 cut(s) 122, 777
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EciI GGCGGA 1 cut(s) 308
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 312, 443
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 284, 440
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 436, 547
EcoRV GATATC 1 cut(s) 585
EcoT14I CCWWGG 2 cut(s) 312, 443
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 312, 443
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 8 cut(s) 316, 352, 382, 447, 485, 775, 784, 805
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FatI CATG 8 cut(s) 312, 348, 378, 443, 481, 771, 780, 801
Fnu4HI GCNGC 3 cut(s) 10, 13, 474
FokI GGATG 2 cut(s) 57, 367
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 10, 13, 474
GlaI GCGC 1 cut(s) 78
GluI GCNGC 3 cut(s) 10, 13, 474
GsaI CCCAGC 1 cut(s) 453
HapII CCGG 2 cut(s) 267, 737
HgaI GACGC 3 cut(s) 31, 372, 644
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 2 cut(s) 23, 383
Hin1II CATG 8 cut(s) 316, 352, 382, 447, 485, 775, 784, 805
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 5 cut(s) 60, 118, 155, 298, 358
HpaII CCGG 2 cut(s) 267, 737
Hpy166II GTNNAC 2 cut(s) 409, 819
Hpy188I TCNGA 3 cut(s) 193, 243, 284
Hpy188III TCNNGA 1 cut(s) 802
Hpy8I GTNNAC 2 cut(s) 409, 819
Hpy99I CGWCG 2 cut(s) 20, 118
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 3 cut(s) 627, 709, 769
HpyCH4V TGCA 3 cut(s) 172, 464, 784
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 482
HpyF3I CTNAG 5 cut(s) 51, 192, 387, 577, 822
Hsp92I GRCGYC 2 cut(s) 23, 383
Hsp92II CATG 8 cut(s) 316, 352, 382, 447, 485, 775, 784, 805
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 2 cut(s) 122, 777
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 3 cut(s) 21, 24, 460
LweI GCATC 1 cut(s) 473
MaeIII GTNAC 1 cut(s) 65
MalI GATC 2 cut(s) 124, 779
MboI GATC 2 cut(s) 122, 777
MboII GAAGA 3 cut(s) 142, 470, 736
MhlI GDGCHC 2 cut(s) 180, 342
MluCI AATT 5 cut(s) 143, 233, 303, 598, 608
MluI ACGCGT 1 cut(s) 69
MlyI GAGTC 2 cut(s) 127, 352
MmeI TCCRAC 1 cut(s) 486
MnlI CCTC 5 cut(s) 50, 345, 384, 545, 625
MseI TTAA 3 cut(s) 258, 500, 513
MslI CAYNNNNRTG 2 cut(s) 335, 353
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 2 cut(s) 267, 737
MspR9I CCNGG 2 cut(s) 438, 549
MvaI CCWGG 2 cut(s) 438, 549
MvnI CGCG 2 cut(s) 71, 79
MwoI GCNNNNNNNGC 2 cut(s) 470, 482
NcoI CCATGG 2 cut(s) 312, 443
NdeII GATC 2 cut(s) 122, 777
NlaIII CATG 8 cut(s) 316, 352, 382, 447, 485, 775, 784, 805
NlaIV GGNNCC 2 cut(s) 346, 435
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 65
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 801
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PfeI GAWTC 3 cut(s) 60, 155, 298
PflMI CCANNNNNTGG 1 cut(s) 449
PkrI GCNGC 3 cut(s) 11, 14, 475
Ple19I CGATCG 1 cut(s) 125
PleI GAGTC 2 cut(s) 126, 352
PpsI GAGTC 2 cut(s) 126, 352
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 436, 547
PspFI CCCAGC 1 cut(s) 449
PspGI CCWGG 2 cut(s) 436, 547
PspN4I GGNNCC 2 cut(s) 346, 435
PspPI GGNCC 2 cut(s) 284, 440
PvuI CGATCG 1 cut(s) 125
RsaI GTAC 3 cut(s) 372, 546, 746
RsaNI GTAC 3 cut(s) 371, 545, 745
RseI CAYNNNNRTG 2 cut(s) 335, 353
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 3 cut(s) 258, 500, 513
SatI GCNGC 3 cut(s) 10, 13, 474
Sau3AI GATC 2 cut(s) 122, 777
Sau96I GGNCC 2 cut(s) 284, 440
SchI GAGTC 2 cut(s) 127, 352
ScrFI CCNGG 2 cut(s) 438, 549
SduI GDGCHC 2 cut(s) 180, 342
SfaNI GCATC 1 cut(s) 473
SfcI CTRYAG 2 cut(s) 750, 765
SinI GGWCC 2 cut(s) 284, 440
SmiMI CAYNNNNRTG 2 cut(s) 335, 353
SmlI CTYRAG 2 cut(s) 360, 512
SmoI CTYRAG 2 cut(s) 360, 512
Sse9I AATT 5 cut(s) 143, 233, 303, 598, 608
SsiI CCGC 1 cut(s) 293
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 436, 547
StyI CCWWGG 2 cut(s) 312, 443
TaaI ACNGT 3 cut(s) 627, 709, 769
TaqI TCGA 6 cut(s) 121, 180, 218, 228, 587, 630
TaqII GACCGA 1 cut(s) 53
TasI AATT 5 cut(s) 143, 233, 303, 598, 608
TfiI GAWTC 3 cut(s) 60, 155, 298
Tru1I TTAA 3 cut(s) 258, 500, 513
Tru9I TTAA 3 cut(s) 258, 500, 513
TscAI CASTG 2 cut(s) 33, 281
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 3 cut(s) 9, 12, 473
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 4 cut(s) 470, 790, 818, 848
TspGWI ACGGA 2 cut(s) 632, 703
TspRI CASTG 2 cut(s) 33, 281
Van91I CCANNNNNTGG 1 cut(s) 449
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 284, 440
XapI RAATTY 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.