MD01G1022400.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013333
Physical Location & Seq
Forward (+)
8956664 .. 8959052
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1022400.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGAGGTGTACGGGCAGCAAGTGGTTCTGTTAACTGGGTGCTCGGAAGGGGGGATAGGTCACGCGCTGGCACGTGCGTTCGCCGCAGAGGGTTGCGTGGTGGTGGCGACGAGCAGGTCAGTGAGATCGATGGCGGATCTTGAAGGGGACTCGCGATTTTATTTGCAAGAGTTGGACGTGGTTTCCGAGGAGAGCGTGGAGAGGGTGGTGTCGAATGTGTTAGAGAAGTATGGCCGAATTGATGTGGTGGTTAACAACGCTGGGGTTCATTGTGTTGGTCCACTCGCTGAGGTCCCTCTCTCTGCTCTCCAACACACTTTTAACACCAATGTTTTCGGTTCCATGAGGTTGATACAAGCTGTTATCCCCCACATGGCATCAAGAAGAAAAGGAAAGATTGTAAATGTTGGAAGTGTTACTGTTGCGGCGCCAACACCGTGGGCTGGTGCTTACACAGCATCCAAAGCTGCTCTTCATGCACTCAGTGATTCTTTAAGATTGGAACTGAGGCCCTTTGGAATCAGTGTGATCACGGTTGTACCTGGAGGAATTAGATCAAACATCGGACGTACTGCTTTGGCCAGCTACAACCAGATGCCCGAATGGAAATTATACAAGCCTTTCGAAGCAGCAATCCGCGCCAGAGCCACTAGCTCACAAGGTCCTAAATCAACCCCATCAGACGAGTTTGCAAAGAAGACTGTGGCTGCCATTCTGCAGAAGAATCCGCCGGCTTGGTTCTCCTACGGTCACCTCGCCACCATTTTCGCCATCTTATATCATCTGCCACTCTTCGTTAAAGATTTTGTATGGAGGAAAGCAATGAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

278

Amino Acids

29.99

Weight (kDa)

9.39

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 7 - 191 5.1e-49 short chain dehydrogenase
KR PF08659 9 - 166 4.3e-09 KR domain
Epimerase PF01370 9 - 145 2e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 16 - 208 1.5e-35 Enoyl-(Acyl carrier protein) reductase
SDR PF23441 60 - 194 6e-07 SDR-like rossmann domain
DUF1776 PF08643 90 - 185 4e-07 Fungal family of unknown function (DUF1776)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10050 AT5G65205
fragaria_vesca FvH4_1g21770 FvH4_1g21770
malus_domestica MD01G1022400.v1.1
prunus_persica Prupe.6G178200_v2.0.a1
pyrus_communis pycom01g05090
rosa_chinensis RchiOBHm_Chr2g0115641
rosa_laevigata RLG00000018200
rosa_multiflora Rmu_ssc0000197.1_g000085
rosa_roxburghii Rroxscaffold_2G00128030 Rroxscaffold_2G00128900
rosa_rugosa Rorug02G0199000
rosa_samantha Rh2AG256100 Rh2BG266900 Rh2CG261500 Rh2DG264400
rosa_wichuraiana Rw2G020050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 115
Acc36I ACCTGC 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 3 cut(s) 65, 154, 639
AciI CCGC 5 cut(s) 84, 134, 425, 637, 728
AclWI GGATC 1 cut(s) 144
AcoI YGGCCR 2 cut(s) 232, 579
AcvI CACGTG 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 428
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 3 cut(s) 11, 540, 571
AfiI CCNNNNNNNGG 2 cut(s) 373, 443
AgsI TTSAA 1 cut(s) 143
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 1 cut(s) 541
AluBI AGCT 4 cut(s) 359, 467, 585, 654
AluI AGCT 4 cut(s) 359, 467, 585, 654
Alw21I GWGCWC 1 cut(s) 44
AlwI GGATC 1 cut(s) 144
AoxI GGCC 3 cut(s) 232, 509, 579
ApeKI GCWGC 4 cut(s) 16, 467, 629, 707
AspLEI GCGC 3 cut(s) 67, 430, 641
AspS9I GGNCC 4 cut(s) 278, 292, 510, 662
AsuHPI GGTGA 1 cut(s) 743
AsuII TTCGAA 1 cut(s) 624
AvaII GGWCC 3 cut(s) 278, 292, 662
BaeI ACNNNNGTAYC 2 cut(s) 522, 555
BalI TGGCCA 1 cut(s) 581
BanI GGYRCC 1 cut(s) 427
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 704
Bbv12I GWGCWC 1 cut(s) 44
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 28, 454, 641, 694
BccI CCATC 3 cut(s) 124, 685, 779
BciT130I CCWGG 1 cut(s) 543
BclI TGATCA 1 cut(s) 528
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 1 cut(s) 716
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 105
BisI GCNGC 6 cut(s) 17, 84, 426, 468, 630, 708
BlsI GCNGC 6 cut(s) 18, 85, 427, 469, 631, 709
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 3 cut(s) 278, 292, 662
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 4 cut(s) 278, 292, 510, 662
BmiI GGNNCC 3 cut(s) 294, 340, 429
BmrFI CCNGG 1 cut(s) 543
BmrI ACTGGG 1 cut(s) 45
BmsI GCATC 3 cut(s) 386, 467, 585
BmuI ACTGGG 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 704
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 288
Bpu14I TTCGAA 1 cut(s) 624
Bsa29I ATCGAT 1 cut(s) 128
BsaAI YACGTR 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 186, 437
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 443
Bse118I RCCGGY 1 cut(s) 730
Bse1I ACTGG 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 1 cut(s) 543
BseCI ATCGAT 1 cut(s) 128
BseDI CCNNGG 2 cut(s) 186, 437
BseGI GGATG 1 cut(s) 458
BseLI CCNNNNNNNGG 2 cut(s) 373, 443
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 3 cut(s) 279, 496, 497
BseNI ACTGG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 4 cut(s) 28, 454, 641, 694
BseYI CCCAGC 1 cut(s) 260
Bsh1236I CGCG 3 cut(s) 65, 154, 639
BshFI GGCC 3 cut(s) 234, 511, 581
BshNI GGYRCC 1 cut(s) 427
BshVI ATCGAT 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 2 cut(s) 161, 278
BslI CCNNNNNNNGG 2 cut(s) 373, 443
BsmFI GGGAC 2 cut(s) 161, 278
BsnI GGCC 3 cut(s) 234, 511, 581
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 4 cut(s) 125, 136, 528, 554
Bsp68I TCGCGA 1 cut(s) 154
BspACI CCGC 5 cut(s) 84, 134, 425, 637, 728
BspANI GGCC 3 cut(s) 234, 511, 581
BspCNI CTCAG 3 cut(s) 280, 495, 498
BspDI ATCGAT 1 cut(s) 128
BspFNI CGCG 3 cut(s) 65, 154, 639
BspLI GGNNCC 3 cut(s) 294, 340, 429
BspMAI CTGCAG 1 cut(s) 720
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 1 cut(s) 144
BspQI GCTCTTC 1 cut(s) 477
BspT104I TTCGAA 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 730
BsrI ACTGG 1 cut(s) 40
BssAI RCCGGY 1 cut(s) 730
BssECI CCNNGG 2 cut(s) 186, 437
BssMI GATC 4 cut(s) 125, 136, 528, 554
BssNI GRCGYC 1 cut(s) 428
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 5 cut(s) 421, 438, 535, 703, 749
Bst6I CTCTTC 2 cut(s) 477, 797
BstACI GRCGYC 1 cut(s) 428
BstBAI YACGTR 1 cut(s) 74
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 3 cut(s) 69, 583, 732
BstDEI CTNAG 3 cut(s) 288, 482, 506
BstDSI CCRYGG 1 cut(s) 437
BstEII GGTNACC 1 cut(s) 749
BstF5I GGATG 1 cut(s) 458
BstFNI CGCG 3 cut(s) 65, 154, 639
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 3 cut(s) 67, 430, 641
BstKTI GATC 4 cut(s) 128, 139, 531, 557
BstMBI GATC 4 cut(s) 125, 136, 528, 554
BstMWI GCNNNNNNNGC 5 cut(s) 83, 455, 464, 476, 638
BstNI CCWGG 1 cut(s) 543
BstPI GGTNACC 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 541
BstSFI CTRYAG 1 cut(s) 716
BstUI CGCG 3 cut(s) 65, 154, 639
BstV1I GCAGC 4 cut(s) 28, 454, 641, 694
BstV2I GAAGAC 1 cut(s) 704
BstX2I RGATCY 1 cut(s) 136
BstXI CCANNNNNNTGG 1 cut(s) 438
BstYI RGATCY 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 128
BsuRI GGCC 3 cut(s) 234, 511, 581
BsuTUI ATCGAT 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 437
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 1 cut(s) 458
BtsIMutI CAGTG 3 cut(s) 126, 490, 529
BtuMI TCGCGA 1 cut(s) 154
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 3 cut(s) 69, 583, 732
CfoI GCGC 3 cut(s) 67, 430, 641
Cfr10I RCCGGY 1 cut(s) 730
Cfr13I GGNCC 4 cut(s) 278, 292, 510, 662
ClaI ATCGAT 1 cut(s) 128
Csp6I GTAC 3 cut(s) 10, 539, 570
CviAII CATG 3 cut(s) 343, 373, 476
CviQI GTAC 3 cut(s) 10, 539, 570
DdeI CTNAG 3 cut(s) 288, 482, 506
DinI GGCGCC 1 cut(s) 429
DpnI GATC 4 cut(s) 127, 138, 530, 556
DpnII GATC 4 cut(s) 125, 136, 528, 554
DraIII CACNNNGTG 1 cut(s) 485
DrdI GACNNNNNNGTC 1 cut(s) 115
DseDI GACNNNNNNGTC 1 cut(s) 115
EaeI YGGCCR 2 cut(s) 232, 579
Eam1104I CTCTTC 2 cut(s) 477, 797
EarI CTCTTC 2 cut(s) 477, 797
EciI GGCGGA 2 cut(s) 149, 717
Eco47I GGWCC 3 cut(s) 278, 292, 662
Eco72I CACGTG 1 cut(s) 74
Eco91I GGTNACC 1 cut(s) 749
EcoO109I RGGNCCY 3 cut(s) 292, 510, 662
EcoO65I GGTNACC 1 cut(s) 749
EcoRII CCWGG 1 cut(s) 541
EgeI GGCGCC 1 cut(s) 429
EheI GGCGCC 1 cut(s) 429
FaeI CATG 3 cut(s) 346, 376, 479
FaiI YATR 7 cut(s) 231, 344, 374, 477, 613, 778, 811
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 2 cut(s) 161, 278
FatI CATG 3 cut(s) 342, 372, 475
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 6 cut(s) 17, 84, 426, 468, 630, 708
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 6 cut(s) 17, 84, 426, 468, 630, 708
FspBI CTAG 1 cut(s) 651
GlaI GCGC 3 cut(s) 66, 429, 640
GluI GCNGC 6 cut(s) 17, 84, 426, 468, 630, 708
GsaI CCCAGC 1 cut(s) 264
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 3 cut(s) 234, 511, 581
HapII CCGG 1 cut(s) 731
HhaI GCGC 3 cut(s) 67, 430, 641
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 3 cut(s) 346, 376, 479
Hin6I GCGC 3 cut(s) 65, 428, 639
HinP1I GCGC 3 cut(s) 65, 428, 639
HincII GTYRAC 2 cut(s) 33, 253
HindII GTYRAC 2 cut(s) 33, 253
HinfI GANTC 4 cut(s) 149, 488, 519, 724
HpaI GTTAAC 2 cut(s) 33, 253
HpaII CCGG 1 cut(s) 731
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 4 cut(s) 10, 33, 253, 281
Hpy188I TCNGA 4 cut(s) 46, 187, 566, 682
Hpy188III TCNNGA 3 cut(s) 140, 153, 381
Hpy8I GTNNAC 4 cut(s) 10, 33, 253, 281
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 2 cut(s) 41, 137
HpyCH4III ACNGT 5 cut(s) 421, 438, 535, 703, 749
HpyCH4IV ACGT 3 cut(s) 73, 177, 568
HpyCH4V TGCA 4 cut(s) 166, 479, 692, 718
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 455, 464, 476, 638
HpyF3I CTNAG 3 cut(s) 288, 482, 506
HpySE526I ACGT 3 cut(s) 73, 177, 568
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 3 cut(s) 346, 376, 479
HspAI GCGC 3 cut(s) 65, 428, 639
KasI GGCGCC 1 cut(s) 427
KroI GCCGGC 1 cut(s) 730
KroNI GCCGGC 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 528
KspAI GTTAAC 2 cut(s) 33, 253
Kzo9I GATC 4 cut(s) 125, 136, 528, 554
LguI GCTCTTC 1 cut(s) 477
Lsp1109I GCAGC 4 cut(s) 28, 454, 641, 694
LweI GCATC 3 cut(s) 386, 467, 585
MaeI CTAG 1 cut(s) 651
MaeII ACGT 3 cut(s) 73, 177, 568
MaeIII GTNAC 3 cut(s) 59, 415, 749
MalI GATC 4 cut(s) 127, 138, 530, 556
MboI GATC 4 cut(s) 125, 136, 528, 554
MboII GAAGA 5 cut(s) 396, 464, 709, 733, 784
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 3 cut(s) 237, 549, 608
MluNI TGGCCA 1 cut(s) 581
Mly113I GGCGCC 1 cut(s) 428
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 3 cut(s) 153, 334, 388
Mox20I TGGCCA 1 cut(s) 581
MroNI GCCGGC 1 cut(s) 730
MscI TGGCCA 1 cut(s) 581
MseI TTAA 5 cut(s) 32, 252, 321, 494, 798
Msp20I TGGCCA 1 cut(s) 581
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 1 cut(s) 543
MvaI CCWGG 1 cut(s) 543
MvnI CGCG 3 cut(s) 65, 154, 639
MwoI GCNNNNNNNGC 5 cut(s) 83, 455, 464, 476, 638
NaeI GCCGGC 1 cut(s) 732
NarI GGCGCC 1 cut(s) 428
NdeII GATC 4 cut(s) 125, 136, 528, 554
NgoMIV GCCGGC 1 cut(s) 730
NlaIII CATG 3 cut(s) 346, 376, 479
NlaIV GGNNCC 3 cut(s) 294, 340, 429
NmuCI GTSAC 2 cut(s) 59, 749
NruI TCGCGA 1 cut(s) 154
NspV TTCGAA 1 cut(s) 624
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 732
PfeI GAWTC 3 cut(s) 488, 519, 724
PkrI GCNGC 6 cut(s) 18, 85, 427, 469, 631, 709
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 1 cut(s) 431
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 74
PpuMI RGGWCCY 2 cut(s) 292, 662
Psp5II RGGWCCY 2 cut(s) 292, 662
Psp6I CCWGG 1 cut(s) 541
PspCI CACGTG 1 cut(s) 74
PspEI GGTNACC 1 cut(s) 749
PspFI CCCAGC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 3 cut(s) 294, 340, 429
PspPI GGNCC 4 cut(s) 278, 292, 510, 662
PspPPI RGGWCCY 2 cut(s) 292, 662
PstI CTGCAG 1 cut(s) 720
PsuI RGATCY 1 cut(s) 136
RruI TCGCGA 1 cut(s) 154
RsaI GTAC 3 cut(s) 11, 540, 571
RsaNI GTAC 3 cut(s) 10, 539, 570
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 5 cut(s) 32, 252, 321, 494, 798
SatI GCNGC 6 cut(s) 17, 84, 426, 468, 630, 708
Sau3AI GATC 4 cut(s) 125, 136, 528, 554
Sau96I GGNCC 4 cut(s) 278, 292, 510, 662
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 3 cut(s) 386, 467, 585
SfcI CTRYAG 1 cut(s) 716
SfoI GGCGCC 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 3 cut(s) 278, 292, 662
Sse9I AATT 3 cut(s) 237, 549, 608
SsiI CCGC 5 cut(s) 84, 134, 425, 637, 728
SspDI GGCGCC 1 cut(s) 427
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 1 cut(s) 541
TaaI ACNGT 5 cut(s) 421, 438, 535, 703, 749
TaiI ACGT 3 cut(s) 76, 180, 571
TaqI TCGA 3 cut(s) 128, 212, 624
TasI AATT 3 cut(s) 237, 549, 608
TauI GCSGC 2 cut(s) 86, 428
TfiI GAWTC 3 cut(s) 488, 519, 724
Tru1I TTAA 5 cut(s) 32, 252, 321, 494, 798
Tru9I TTAA 5 cut(s) 32, 252, 321, 494, 798
TscAI CASTG 3 cut(s) 126, 490, 529
TseFI GTSAC 2 cut(s) 59, 749
TseI GCWGC 4 cut(s) 16, 467, 629, 707
Tsp45I GTSAC 2 cut(s) 59, 749
TspDTI ATGAA 2 cut(s) 257, 464
TspRI CASTG 3 cut(s) 126, 490, 529
VpaK11BI GGWCC 3 cut(s) 278, 292, 662
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.