Rh2DG264400

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013333
Physical Location & Seq
Reverse (-)
28471347 .. 28475807
4461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG264400.1

Sequence Viewer

Length: 834 bp
ATGGAGTTCTACGGCCAACAAGTGGTTCTGATCACCGGCTGCTCGCAGGGTGGCATCGGCCACGCGCTGGCACGCGCCTTCGCCGACGAGGGCTGCCTGGTGGTGGCCACAGCCAGGTCTCTGAGCTCCATGGCCTATCTTGAGGACGACCCAAGATTCTTCCTTCAAGAATTGGACGTGGTTTCCGAGGAGAGCGTGAACGAAGTGGTGTCTACAGTGGTGGAGAAGTACGGCAGAATCGATGTGGTGGTGAACAATGCTGGAGTTCAGTGCGTGGGTCCACTCGCTGAAGTTCCTCTCTCTGCTATGCAACACACGTTTGACACCAACGTTTATGGTCCCATGAGGTTGATACAAGCTGTTATCCCTCACATGGTGTCAAGGAGAAAGGGAAAGATTGTAAATGTTGGAAGTGTTACTGCGTTGTCCCCTGGACCATGGTCTGGTGCTTACACAGCATCTAAAGCTGCTCTTCAAGCACTTAGTGATTCTTTAAGATTGGAACTCAAGCCCTTTGGGATCAATGTAATCACTGTTGTACCTGGAGCTATTAGGTCAAACATTGGAAATTCTGGTTTAGTTGGCTACAACAAGATGCCTGAATGGAAATTATATAAACCTTTTGAGGCAGCAATTCGTGCTAGAGTGACTCTCTCACAAGGACCCAAATCAACCCCTTCAGAGGAGTTTGCGAAGAAGACAGTATCTGCCATTCTGAAGAAGAATCCACCAGCTTGGTTCTCTTCCGGTCACCTCTCTGTCATATTTGCAATCTTGTATTATATGCCAATCGTCGTTAGGGATTTTGTCCTGAGGAAAGCAATGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

277

Amino Acids

30.03

Weight (kDa)

9.18

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 7 - 189 3.7e-51 short chain dehydrogenase
KR PF08659 9 - 166 2.1e-08 KR domain
Epimerase PF01370 9 - 142 3.8e-07 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 16 - 199 1.9e-36 Enoyl-(Acyl carrier protein) reductase
SDR PF23441 63 - 190 7.3e-06 SDR-like rossmann domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10050 AT5G65205
fragaria_vesca FvH4_1g21770 FvH4_1g21770
malus_domestica MD01G1022400.v1.1
prunus_persica Prupe.6G178200_v2.0.a1
pyrus_communis pycom01g05090
rosa_chinensis RchiOBHm_Chr2g0115641
rosa_laevigata RLG00000018200
rosa_multiflora Rmu_ssc0000197.1_g000085
rosa_roxburghii Rroxscaffold_2G00128030 Rroxscaffold_2G00128900
rosa_rugosa Rorug02G0199000
rosa_samantha Rh2AG256100 Rh2BG266900 Rh2CG261500 Rh2DG264400
rosa_wichuraiana Rw2G020050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 22, 67, 443
AccI GTMKAC 1 cut(s) 212
AccII CGCG 2 cut(s) 65, 75
AclI AACGTT 1 cut(s) 330
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 3 cut(s) 13, 58, 105
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 3 cut(s) 309, 663, 737
AdeI CACNNNGTG 2 cut(s) 376, 485
AfaI GTAC 2 cut(s) 230, 540
AfiI CCNNNNNNNGG 7 cut(s) 22, 67, 103, 114, 373, 443, 682
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 2 cut(s) 167, 476
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 4 cut(s) 96, 113, 430, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 6 cut(s) 126, 359, 467, 548, 734, 829
AluI AGCT 6 cut(s) 126, 359, 467, 548, 734, 829
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 1 cut(s) 527
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 4 cut(s) 13, 58, 105, 132
ApeKI GCWGC 4 cut(s) 39, 93, 467, 629
ApoI RAATTY 1 cut(s) 568
AspLEI GCGC 2 cut(s) 67, 77
AspS9I GGNCC 4 cut(s) 278, 338, 434, 662
AsuHPI GGTGA 3 cut(s) 25, 262, 743
AvaII GGWCC 4 cut(s) 278, 338, 434, 662
AxyI CCTNAGG 1 cut(s) 812
BaeI ACNNNNGTAYC 2 cut(s) 522, 555
BalI TGGCCA 1 cut(s) 107
BanII GRGCYC 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 704
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 4 cut(s) 26, 80, 454, 641
BceAI ACGGC 2 cut(s) 28, 247
BciT130I CCWGG 4 cut(s) 98, 115, 432, 543
BclI TGATCA 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 1 cut(s) 213
BisI GCNGC 4 cut(s) 40, 94, 468, 630
BlsI GCNGC 4 cut(s) 41, 95, 469, 631
Bme1390I CCNGG 4 cut(s) 98, 115, 432, 543
Bme18I GGWCC 4 cut(s) 278, 338, 434, 662
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 4 cut(s) 278, 338, 434, 662
BmiI GGNNCC 3 cut(s) 279, 340, 664
BmrFI CCNGG 4 cut(s) 98, 115, 432, 543
BmsI GCATC 3 cut(s) 63, 467, 585
BoxI GACNNNNGTC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 704
BplI GAGNNNNNCTC 2 cut(s) 636, 668
BpmI CTGGAG 2 cut(s) 282, 564
BpuEI CTTGAG 2 cut(s) 161, 491
Bsa29I ATCGAT 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 129, 186, 430, 437
BsaWI WCCGGW 1 cut(s) 746
Bsc4I CCNNNNNNNGG 7 cut(s) 22, 67, 103, 114, 373, 443, 682
Bse118I RCCGGY 1 cut(s) 35
Bse21I CCTNAGG 1 cut(s) 812
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 4 cut(s) 98, 115, 432, 543
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 4 cut(s) 129, 186, 430, 437
BseLI CCNNNNNNNGG 7 cut(s) 22, 67, 103, 114, 373, 443, 682
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 2 cut(s) 113, 803
BseRI GAGGAG 2 cut(s) 203, 698
BseXI GCAGC 4 cut(s) 26, 80, 454, 641
Bsh1236I CGCG 2 cut(s) 65, 75
BshFI GGCC 4 cut(s) 15, 60, 107, 134
BshVI ATCGAT 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 2 cut(s) 36, 747
BslFI GGGAC 2 cut(s) 324, 412
BslI CCNNNNNNNGG 7 cut(s) 22, 67, 103, 114, 373, 443, 682
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 324, 412
BsnI GGCC 4 cut(s) 15, 60, 107, 134
Bso31I GGTCTC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 2 cut(s) 30, 519
Bsp19I CCATGG 2 cut(s) 129, 437
BspANI GGCC 4 cut(s) 15, 60, 107, 134
BspCNI CTCAG 2 cut(s) 114, 804
BspDI ATCGAT 1 cut(s) 240
BspFNI CGCG 2 cut(s) 65, 75
BspLI GGNNCC 3 cut(s) 279, 340, 664
BspPI GGATC 1 cut(s) 527
BspQI GCTCTTC 1 cut(s) 477
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 4 cut(s) 129, 186, 430, 437
BssMI GATC 2 cut(s) 30, 519
BssT1I CCWWGG 2 cut(s) 129, 437
Bst2UI CCWGG 4 cut(s) 98, 115, 432, 543
Bst4CI ACNGT 3 cut(s) 217, 535, 703
Bst6I CTCTTC 2 cut(s) 477, 748
BstAPI GCANNNNNTGC 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 44, 69, 73
BstDEI CTNAG 3 cut(s) 122, 482, 812
BstDSI CCRYGG 2 cut(s) 129, 437
BstEII GGTNACC 1 cut(s) 749
BstFNI CGCG 2 cut(s) 65, 75
BstHHI GCGC 2 cut(s) 67, 77
BstKTI GATC 2 cut(s) 33, 522
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 30, 519
BstMWI GCNNNNNNNGC 4 cut(s) 455, 464, 476, 638
BstNI CCWGG 4 cut(s) 98, 115, 432, 543
BstPAI GACNNNNGTC 1 cut(s) 439
BstPI GGTNACC 1 cut(s) 749
BstSCI CCNGG 4 cut(s) 96, 113, 430, 541
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 2 cut(s) 65, 75
BstV1I GCAGC 4 cut(s) 26, 80, 454, 641
BstV2I GAAGAC 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 240
Bsu36I CCTNAGG 1 cut(s) 812
BsuRI GGCC 4 cut(s) 15, 60, 107, 134
BsuTUI ATCGAT 1 cut(s) 240
BtgI CCRYGG 2 cut(s) 129, 437
BtrI CACGTC 1 cut(s) 178
BtsIMutI CAGTG 3 cut(s) 222, 275, 531
Cac8I GCNNGC 3 cut(s) 44, 69, 73
CaiI CAGNNNCTG 1 cut(s) 707
CfoI GCGC 2 cut(s) 67, 77
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 4 cut(s) 278, 338, 434, 662
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 2 cut(s) 229, 539
CviAII CATG 4 cut(s) 130, 343, 373, 438
CviQI GTAC 2 cut(s) 229, 539
DdeI CTNAG 3 cut(s) 122, 482, 812
DpnI GATC 2 cut(s) 32, 521
DpnII GATC 2 cut(s) 30, 519
DraIII CACNNNGTG 2 cut(s) 376, 485
EaeI YGGCCR 3 cut(s) 13, 58, 105
Eam1104I CTCTTC 2 cut(s) 477, 748
EarI CTCTTC 2 cut(s) 477, 748
Ecl136II GAGCTC 1 cut(s) 126
Eco130I CCWWGG 2 cut(s) 129, 437
Eco24I GRGCYC 1 cut(s) 128
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 4 cut(s) 278, 338, 434, 662
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 3 cut(s) 309, 663, 737
Eco81I CCTNAGG 1 cut(s) 812
Eco91I GGTNACC 1 cut(s) 749
EcoICRI GAGCTC 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 749
EcoRII CCWGG 4 cut(s) 96, 113, 430, 541
EcoT14I CCWWGG 2 cut(s) 129, 437
EcoT38I GRGCYC 1 cut(s) 128
ErhI CCWWGG 2 cut(s) 129, 437
FaeI CATG 4 cut(s) 133, 346, 376, 441
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 2 cut(s) 324, 412
FatI CATG 4 cut(s) 129, 342, 372, 437
FbaI TGATCA 1 cut(s) 30
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 4 cut(s) 40, 94, 468, 630
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 4 cut(s) 40, 94, 468, 630
FspBI CTAG 1 cut(s) 642
GlaI GCGC 2 cut(s) 66, 76
GluI GCNGC 4 cut(s) 40, 94, 468, 630
GsuI CTGGAG 2 cut(s) 282, 564
HaeIII GGCC 4 cut(s) 15, 60, 107, 134
HapII CCGG 2 cut(s) 36, 747
HhaI GCGC 2 cut(s) 67, 77
Hin1II CATG 4 cut(s) 133, 346, 376, 441
Hin6I GCGC 2 cut(s) 65, 75
HinP1I GCGC 2 cut(s) 65, 75
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 5 cut(s) 156, 237, 488, 649, 724
HpaII CCGG 2 cut(s) 36, 747
HphI GGTGA 3 cut(s) 25, 262, 743
Hpy166II GTNNAC 4 cut(s) 199, 213, 253, 281
Hpy188I TCNGA 5 cut(s) 30, 123, 187, 682, 717
Hpy188III TCNNGA 3 cut(s) 140, 167, 811
Hpy8I GTNNAC 4 cut(s) 199, 213, 253, 281
Hpy99I CGWCG 2 cut(s) 89, 797
HpyAV CCTTC 3 cut(s) 88, 173, 687
HpyCH4III ACNGT 3 cut(s) 217, 535, 703
HpyCH4IV ACGT 3 cut(s) 177, 317, 330
HpyCH4V TGCA 2 cut(s) 310, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 455, 464, 476, 638
HpyF3I CTNAG 3 cut(s) 122, 482, 812
HpySE526I ACGT 3 cut(s) 177, 317, 330
Hsp92II CATG 4 cut(s) 133, 346, 376, 441
HspAI GCGC 2 cut(s) 65, 75
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 2 cut(s) 30, 519
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 2 cut(s) 131, 545
Lsp1109I GCAGC 4 cut(s) 26, 80, 454, 641
LweI GCATC 3 cut(s) 63, 467, 585
MaeI CTAG 1 cut(s) 642
MaeII ACGT 3 cut(s) 177, 317, 330
MaeIII GTNAC 3 cut(s) 415, 646, 749
MalI GATC 2 cut(s) 32, 521
MboI GATC 2 cut(s) 30, 519
MboII GAAGA 7 cut(s) 151, 464, 706, 709, 730, 733, 735
MhlI GDGCHC 1 cut(s) 128
MlsI TGGCCA 1 cut(s) 107
MluCI AATT 4 cut(s) 170, 568, 608, 633
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 1 cut(s) 643
MmeI TCCRAC 1 cut(s) 388
Mox20I TGGCCA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 107
MseI TTAA 1 cut(s) 494
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 2 cut(s) 36, 747
MspR9I CCNGG 4 cut(s) 98, 115, 432, 543
MvaI CCWGG 4 cut(s) 98, 115, 432, 543
MvnI CGCG 2 cut(s) 65, 75
MwoI GCNNNNNNNGC 4 cut(s) 455, 464, 476, 638
NcoI CCATGG 2 cut(s) 129, 437
NdeII GATC 2 cut(s) 30, 519
NlaIII CATG 4 cut(s) 133, 346, 376, 441
NlaIV GGNNCC 3 cut(s) 279, 340, 664
NmuCI GTSAC 2 cut(s) 646, 749
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 237
PfeI GAWTC 4 cut(s) 156, 237, 488, 724
PflMI CCANNNNNTGG 3 cut(s) 22, 67, 443
PkrI GCNGC 4 cut(s) 41, 95, 469, 631
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 662
PshAI GACNNNNGTC 1 cut(s) 439
Psp124BI GAGCTC 1 cut(s) 128
Psp1406I AACGTT 1 cut(s) 330
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 4 cut(s) 96, 113, 430, 541
PspEI GGTNACC 1 cut(s) 749
PspGI CCWGG 4 cut(s) 96, 113, 430, 541
PspN4I GGNNCC 3 cut(s) 279, 340, 664
PspPI GGNCC 4 cut(s) 278, 338, 434, 662
PspPPI RGGWCCY 1 cut(s) 662
PstNI CAGNNNCTG 1 cut(s) 707
RsaI GTAC 2 cut(s) 230, 540
RsaNI GTAC 2 cut(s) 229, 539
SacI GAGCTC 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 4 cut(s) 40, 94, 468, 630
Sau3AI GATC 2 cut(s) 30, 519
Sau96I GGNCC 4 cut(s) 278, 338, 434, 662
SchI GAGTC 1 cut(s) 643
ScrFI CCNGG 4 cut(s) 98, 115, 432, 543
SduI GDGCHC 1 cut(s) 128
SfaNI GCATC 3 cut(s) 63, 467, 585
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 4 cut(s) 278, 338, 434, 662
SmlI CTYRAG 2 cut(s) 140, 506
SmoI CTYRAG 2 cut(s) 140, 506
Sse9I AATT 4 cut(s) 170, 568, 608, 633
SspMI CTAG 1 cut(s) 642
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 4 cut(s) 96, 113, 430, 541
StyI CCWWGG 2 cut(s) 129, 437
TaaI ACNGT 3 cut(s) 217, 535, 703
TaiI ACGT 3 cut(s) 180, 320, 333
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 170, 568, 608, 633
TfiI GAWTC 4 cut(s) 156, 237, 488, 724
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 3 cut(s) 222, 275, 538
TseFI GTSAC 2 cut(s) 646, 749
TseI GCWGC 4 cut(s) 39, 93, 467, 629
Tsp45I GTSAC 2 cut(s) 646, 749
TspRI CASTG 3 cut(s) 222, 275, 538
Van91I CCANNNNNTGG 3 cut(s) 22, 67, 443
VpaK11BI GGWCC 4 cut(s) 278, 338, 434, 662
XapI RAATTY 1 cut(s) 568
XmiI GTMKAC 1 cut(s) 212
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.