AT5G66330

Leucine rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010221
Physical Location & Seq
Reverse (-)
26500402 .. 26501955
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66330.1

Sequence Viewer

Length: 1257 bp
ATGGCTTCACTTATTTCCCTTGTTCTTCTTCTTTTGCTCACTTTATCTTCAGCAGAGTCCAAGACTTTCTGGGGTGACGTGGCAGCTCTCAAAGACTTCAAGAACTCAGTGGATGCCAAATCCATGAGTCCAGGCTCTTGCCTGAGTTCTTGGGATTTCTCCGTCGATCCCTGCGACAATATATTCAGCGACACATTCACTTGTGGGTTCCGCTGCGACTCCGTCGTTACCGGATCGGGTCGGGTCACGGAGCTCAGTCTGGACCAAGCCGGTTACTCAGGCTCCCTCTCCTCCGTCTCTTTCAACCTTCCTTATCTCCAGACCCTCGATCTCTCCGGCAACTACTTCTCCGGTCCACTTCCCGACTCGCTCTCCAATCTCACCCGTCTCACCCGCCTCACTGTGTCCGGAAACTCCTTTTCCGGATCCATACCCGATTCAGTCGGGTCAATGACGGTTCTTGAGGAGCTCGTGCTAGACAGCAACCGCCTCTACGGCTCCATTCCGGCGAGCTTCAATGGCCTTTCCAGCCTGAAACGACTGGAAATCCAGCTAAACAACATCTCCGGCGAATTCCCTGATCTGAGCTCACTCAAAAACCTGTATTACCTCGACGCGAGCGATAACCGCATCTCCGGTCGGATCCCATCATTTTTACCCGAATCTATAGTCCAAATCTCAATGCGAAACAACCTCTTCCAAGGAACGATCCCTGAAAGCTTCAAACTTCTAAATTCTCTCGAAGTAATCGATCTTAGCCACAACAAACTCAGTGGCTCAATCCCATCGTTTATCTTCACTCATCAGTCTCTACAGCAGCTAACACTCTCCTTCAATGGCTTCACCTCTTTAGAGTCACCCTACTATTCTCCCTTGGGTCTCCCCAGCGAGCTCATATCCGTAGATCTCAGCAACAATCAGATCCTCGGTGCGTTACCTCTGTTCATGGGTCTATCACCAAAGCTCTCAGCATTGTCGTTAGAGAACAACAAGTTCTTTGGTATGATTCCGACCCAGTACGTCTGGAAAACCGTATCGCCCGGATCCGAATTCGCCGGGTTTCAGAGACTTTTACTAGGCGGAAATTTCTTATTCGGAGTCGTACCGGGTCCTTTGATGGCGTTAAAGCCTGGATCAGCGAACGTGCAGCTCGCCGGAAACTGCTTCTCATGGTGTCCGGCGACGTTATTCTTCTGCCAAGGACAAGAACAGAGATCTCCGAAGGAATGTAGAAAGTTTAGCCGCGTCGTCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

418

Amino Acids

45.33

Weight (kDa)

5.1

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 83 - 139 2.7e-06 Leucine rich repeat
LRR_14 PF23598 101 - 201 1.1e-09 Leucine-rich repeat region
LRR_8 PF13855 154 - 211 1.1e-07 Leucine rich repeat
LRR_8 PF13855 227 - 280 2.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 617, 1245
AccIII TCCGGA 2 cut(s) 407, 422
AciI CCGC 6 cut(s) 211, 394, 487, 628, 1080, 1243
AcsI RAATTY 4 cut(s) 572, 733, 1049, 1084
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 2 cut(s) 1019, 1104
AgsI TTSAA 5 cut(s) 100, 304, 517, 724, 835
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 130, 1129
Alw21I GWGCWC 4 cut(s) 255, 471, 590, 894
Alw26I GTCTC 5 cut(s) 301, 392, 813, 884, 1060
Aor13HI TCCGGA 2 cut(s) 407, 422
AoxI GGCC 1 cut(s) 520
ApeKI GCWGC 4 cut(s) 83, 213, 817, 1147
ApoI RAATTY 4 cut(s) 572, 733, 1049, 1084
ArsI GACNNNNNNTTYG 2 cut(s) 654, 686
AspS9I GGNCC 3 cut(s) 262, 353, 1109
AsuC2I CCSGG 3 cut(s) 1041, 1057, 1107
AsuHPI GGTGA 6 cut(s) 86, 373, 382, 835, 849, 948
AvaII GGWCC 3 cut(s) 262, 353, 1109
BamHI GGATCC 3 cut(s) 425, 642, 1043
BanII GRGCYC 4 cut(s) 255, 471, 590, 894
BauI CACGAG 1 cut(s) 470
Bbv12I GWGCWC 4 cut(s) 255, 471, 590, 894
BbvI GCAGC 4 cut(s) 95, 200, 829, 1159
BccI CCATC 3 cut(s) 655, 793, 1111
BceAI ACGGC 1 cut(s) 511
BciT130I CCWGG 2 cut(s) 132, 1131
BcnI CCSGG 3 cut(s) 1041, 1057, 1107
BcoDI GTCTC 5 cut(s) 301, 392, 813, 884, 1060
BfaI CTAG 2 cut(s) 476, 1076
BfmI CTRYAG 2 cut(s) 666, 812
BglI GCCNNNNNGGC 1 cut(s) 495
BglII AGATCT 2 cut(s) 904, 1214
BisI GCNGC 5 cut(s) 84, 214, 818, 1148, 1243
BlsI GCNGC 5 cut(s) 85, 215, 819, 1149, 1244
Bme1390I CCNGG 5 cut(s) 132, 1041, 1057, 1107, 1131
Bme18I GGWCC 3 cut(s) 262, 353, 1109
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 262, 353, 1109
BmiI GGNNCC 7 cut(s) 209, 283, 427, 499, 644, 1045, 1110
BmrFI CCNGG 5 cut(s) 132, 1041, 1057, 1107, 1131
BmrI ACTGGG 1 cut(s) 1009
BmsI GCATC 2 cut(s) 103, 639
BmuI ACTGGG 1 cut(s) 1009
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 3 cut(s) 1041, 1057, 1107
Bsa29I ATCGAT 1 cut(s) 750
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 4 cut(s) 700, 873, 925, 1198
BsaWI WCCGGW 5 cut(s) 230, 350, 407, 422, 635
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 546, 1015
BseAI TCCGGA 2 cut(s) 407, 422
BseBI CCWGG 2 cut(s) 132, 1131
BseCI ATCGAT 1 cut(s) 750
BseDI CCNNGG 4 cut(s) 700, 873, 925, 1198
BseGI GGATG 1 cut(s) 118
BseMII CTCAG 8 cut(s) 120, 134, 268, 291, 575, 784, 922, 981
BseNI ACTGG 2 cut(s) 546, 1015
BseRI GAGGAG 2 cut(s) 280, 479
BseXI GCAGC 4 cut(s) 95, 200, 829, 1159
BseYI CCCAGC 1 cut(s) 884
BsgI GTGCAG 1 cut(s) 1166
Bsh1236I CGCG 2 cut(s) 617, 1245
Bsh1285I CGRYCG 1 cut(s) 640
BshFI GGCC 1 cut(s) 522
BshVI ATCGAT 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 640
BsiHKAI GWGCWC 4 cut(s) 255, 471, 590, 894
BslFI GGGAC 1 cut(s) 1235
BsmAI GTCTC 5 cut(s) 301, 392, 813, 884, 1060
BsmBI CGTCTC 2 cut(s) 301, 392
BsmFI GGGAC 1 cut(s) 1235
BsnI GGCC 1 cut(s) 522
Bso31I GGTCTC 1 cut(s) 884
Bsp1286I GDGCHC 4 cut(s) 255, 471, 590, 894
Bsp13I TCCGGA 2 cut(s) 407, 422
BspACI CCGC 6 cut(s) 211, 394, 487, 628, 1080, 1243
BspANI GGCC 1 cut(s) 522
BspCNI CTCAG 8 cut(s) 119, 135, 267, 290, 576, 783, 921, 980
BspDI ATCGAT 1 cut(s) 750
BspEI TCCGGA 2 cut(s) 407, 422
BspFNI CGCG 2 cut(s) 617, 1245
BspLI GGNNCC 7 cut(s) 209, 283, 427, 499, 644, 1045, 1110
BspTNI GGTCTC 1 cut(s) 884
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 2 cut(s) 546, 1015
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 4 cut(s) 700, 873, 925, 1198
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 3 cut(s) 700, 873, 1198
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 2 cut(s) 132, 1131
Bst4CI ACNGT 3 cut(s) 403, 457, 1033
Bst6I CTCTTC 1 cut(s) 701
BstC8I GCNNGC 4 cut(s) 511, 619, 890, 1152
BstDEI CTNAG 9 cut(s) 106, 143, 254, 277, 584, 755, 770, 908, 967
BstF5I GGATG 1 cut(s) 118
BstFNI CGCG 2 cut(s) 617, 1245
BstMAI GTCTC 5 cut(s) 301, 392, 813, 884, 1060
BstMCI CGRYCG 1 cut(s) 640
BstMWI GCNNNNNNNGC 4 cut(s) 495, 519, 528, 627
BstNI CCWGG 2 cut(s) 132, 1131
BstSCI CCNGG 5 cut(s) 130, 1039, 1055, 1105, 1129
BstSFI CTRYAG 2 cut(s) 666, 812
BstUI CGCG 2 cut(s) 617, 1245
BstV1I GCAGC 4 cut(s) 95, 200, 829, 1159
BstX2I RGATCY 6 cut(s) 425, 642, 904, 921, 1043, 1214
BstYI RGATCY 6 cut(s) 425, 642, 904, 921, 1043, 1214
Bsu15I ATCGAT 1 cut(s) 750
BsuRI GGCC 1 cut(s) 522
BsuTUI ATCGAT 1 cut(s) 750
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 3 cut(s) 114, 399, 778
Cac8I GCNNGC 4 cut(s) 511, 619, 890, 1152
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 262, 353, 1109
ClaI ATCGAT 1 cut(s) 750
CseI GACGC 2 cut(s) 623, 1234
Csp6I GTAC 2 cut(s) 1018, 1103
CspCI CAANNNNNGTGG 2 cut(s) 754, 789
CviAII CATG 3 cut(s) 124, 946, 1170
CviQI GTAC 2 cut(s) 1018, 1103
DdeI CTNAG 9 cut(s) 106, 143, 254, 277, 584, 755, 770, 908, 967
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
EciI GGCGGA 1 cut(s) 1095
Ecl136II GAGCTC 4 cut(s) 253, 469, 588, 892
Eco130I CCWWGG 3 cut(s) 700, 873, 1198
Eco24I GRGCYC 4 cut(s) 255, 471, 590, 894
Eco31I GGTCTC 1 cut(s) 884
Eco47I GGWCC 3 cut(s) 262, 353, 1109
Eco53kI GAGCTC 4 cut(s) 253, 469, 588, 892
Eco57I CTGAAG 1 cut(s) 33
EcoICRI GAGCTC 4 cut(s) 253, 469, 588, 892
EcoO109I RGGNCCY 1 cut(s) 1109
EcoRI GAATTC 2 cut(s) 572, 1049
EcoRII CCWGG 2 cut(s) 130, 1129
EcoT14I CCWWGG 3 cut(s) 700, 873, 1198
EcoT38I GRGCYC 4 cut(s) 255, 471, 590, 894
ErhI CCWWGG 3 cut(s) 700, 873, 1198
Esp3I CGTCTC 2 cut(s) 301, 392
FaeI CATG 3 cut(s) 127, 949, 1173
FaiI YATR 8 cut(s) 125, 182, 431, 668, 896, 947, 1004, 1171
FaqI GGGAC 1 cut(s) 1235
FatI CATG 3 cut(s) 123, 945, 1169
FauI CCCGC 1 cut(s) 401
Fnu4HI GCNGC 5 cut(s) 84, 214, 818, 1148, 1243
FokI GGATG 1 cut(s) 125
FriOI GRGCYC 4 cut(s) 255, 471, 590, 894
Fsp4HI GCNGC 5 cut(s) 84, 214, 818, 1148, 1243
FspBI CTAG 2 cut(s) 476, 1076
GluI GCNGC 5 cut(s) 84, 214, 818, 1148, 1243
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 1 cut(s) 522
HgaI GACGC 2 cut(s) 623, 1234
Hin1II CATG 3 cut(s) 127, 949, 1173
HindIII AAGCTT 1 cut(s) 718
HinfI GANTC 9 cut(s) 56, 127, 218, 365, 437, 662, 854, 1006, 1098
HphI GGTGA 6 cut(s) 86, 373, 382, 835, 849, 948
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 8 cut(s) 585, 642, 921, 1011, 1048, 1065, 1097, 1221
Hpy188III TCNNGA 9 cut(s) 100, 260, 319, 362, 408, 423, 461, 740, 1024
Hpy8I GTNNAC 1 cut(s) 356
Hpy99I CGWCG 5 cut(s) 167, 227, 617, 1186, 1250
HpyAV CCTTC 3 cut(s) 317, 841, 1216
HpyCH4III ACNGT 3 cut(s) 403, 457, 1033
HpyCH4IV ACGT 4 cut(s) 78, 1020, 1143, 1184
HpyCH4V TGCA 1 cut(s) 1147
HpyF10VI GCNNNNNNNGC 4 cut(s) 495, 519, 528, 627
HpyF3I CTNAG 9 cut(s) 106, 143, 254, 277, 584, 755, 770, 908, 967
HpySE526I ACGT 4 cut(s) 78, 1020, 1143, 1184
Hsp92II CATG 3 cut(s) 127, 949, 1173
Kpn2I TCCGGA 2 cut(s) 407, 422
LmnI GCTCC 4 cut(s) 250, 287, 466, 503
Lsp1109I GCAGC 4 cut(s) 95, 200, 829, 1159
LweI GCATC 2 cut(s) 103, 639
MaeI CTAG 2 cut(s) 476, 1076
MaeII ACGT 4 cut(s) 78, 1020, 1143, 1184
MaeIII GTNAC 6 cut(s) 74, 226, 244, 272, 855, 933
MboII GAAGA 6 cut(s) 17, 20, 39, 688, 787, 1183
MflI RGATCY 6 cut(s) 425, 642, 904, 921, 1043, 1214
MhlI GDGCHC 4 cut(s) 255, 471, 590, 894
MluCI AATT 4 cut(s) 572, 733, 1049, 1084
MlyI GAGTC 6 cut(s) 65, 136, 212, 359, 863, 1107
MmeI TCCRAC 2 cut(s) 620, 1034
MroI TCCGGA 2 cut(s) 407, 422
MseI TTAA 1 cut(s) 1124
MspA1I CMGCKG 1 cut(s) 213
MspR9I CCNGG 5 cut(s) 132, 1041, 1057, 1107, 1131
MvaI CCWGG 2 cut(s) 132, 1131
MvnI CGCG 2 cut(s) 617, 1245
MwoI GCNNNNNNNGC 4 cut(s) 495, 519, 528, 627
NciI CCSGG 3 cut(s) 1041, 1057, 1107
NlaIII CATG 3 cut(s) 127, 949, 1173
NlaIV GGNNCC 7 cut(s) 209, 283, 427, 499, 644, 1045, 1110
NmuCI GTSAC 3 cut(s) 74, 244, 855
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 618, 747
PfeI GAWTC 3 cut(s) 437, 662, 1006
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 5 cut(s) 85, 215, 819, 1149, 1244
PleI GAGTC 6 cut(s) 64, 135, 212, 359, 862, 1106
PpsI GAGTC 6 cut(s) 64, 135, 212, 359, 862, 1106
PpuMI RGGWCCY 1 cut(s) 1109
Psp124BI GAGCTC 4 cut(s) 255, 471, 590, 894
Psp5II RGGWCCY 1 cut(s) 1109
Psp6I CCWGG 2 cut(s) 130, 1129
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 2 cut(s) 130, 1129
PspN4I GGNNCC 7 cut(s) 209, 283, 427, 499, 644, 1045, 1110
PspPI GGNCC 3 cut(s) 262, 353, 1109
PspPPI RGGWCCY 1 cut(s) 1109
PsuI RGATCY 6 cut(s) 425, 642, 904, 921, 1043, 1214
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 2 cut(s) 1019, 1104
RsaNI GTAC 2 cut(s) 1018, 1103
SacI GAGCTC 4 cut(s) 255, 471, 590, 894
SaqAI TTAA 1 cut(s) 1124
SatI GCNGC 5 cut(s) 84, 214, 818, 1148, 1243
Sau96I GGNCC 3 cut(s) 262, 353, 1109
SchI GAGTC 6 cut(s) 65, 136, 212, 359, 863, 1107
ScrFI CCNGG 5 cut(s) 132, 1041, 1057, 1107, 1131
SduI GDGCHC 4 cut(s) 255, 471, 590, 894
SfaNI GCATC 2 cut(s) 103, 639
SfcI CTRYAG 2 cut(s) 666, 812
SinI GGWCC 3 cut(s) 262, 353, 1109
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 4 cut(s) 572, 733, 1049, 1084
SsiI CCGC 6 cut(s) 211, 394, 487, 628, 1080, 1243
SspMI CTAG 2 cut(s) 476, 1076
SstI GAGCTC 4 cut(s) 255, 471, 590, 894
StyD4I CCNGG 5 cut(s) 130, 1039, 1055, 1105, 1129
StyI CCWWGG 3 cut(s) 700, 873, 1198
TaaI ACNGT 3 cut(s) 403, 457, 1033
TaiI ACGT 4 cut(s) 81, 1023, 1146, 1187
TaqI TCGA 5 cut(s) 165, 327, 612, 741, 750
TasI AATT 4 cut(s) 572, 733, 1049, 1084
TauI GCSGC 1 cut(s) 1245
TfiI GAWTC 3 cut(s) 437, 662, 1006
Tru1I TTAA 1 cut(s) 1124
Tru9I TTAA 1 cut(s) 1124
TscAI CASTG 3 cut(s) 114, 406, 778
TseFI GTSAC 3 cut(s) 74, 244, 855
TseI GCWGC 4 cut(s) 83, 213, 817, 1147
Tsp45I GTSAC 3 cut(s) 74, 244, 855
TspDTI ATGAA 1 cut(s) 934
TspGWI ACGGA 5 cut(s) 151, 211, 263, 283, 889
TspRI CASTG 3 cut(s) 114, 406, 778
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 3 cut(s) 262, 353, 1109
XapI RAATTY 4 cut(s) 572, 733, 1049, 1084
XspI CTAG 2 cut(s) 476, 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.