Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010221

Physical Location & Seq

Genomic Coordinates Reverse (-)

20904519 .. 20905808

Gene Structure 1290 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1250000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGGGTGTTCAAATACACAATTTGTCTCTGTCCTCCCTGATTATTTTGATAATTTCAGCTGCAAGTCTGCAACTTTTAGCAGAATCCAAGACTTTCTGGGCCGACATTGAAGCTCTCAAGGAGTTCAAGAACTCACTCAACCCCGACTCAGTGACTCCCGGCTCGTGCGTCAACTCCTGGGACTTCACCCTCGACCCCTGCGACAACCTCTTCAGCGACCGGTTCACCTGTGGCTTCCGCTGCGACCTGCTCGACAACTCCTCGTCCGCCAGCCGGCTCACCGAGCTCACCCTTGACCAGGCCGGTTACTCCGGCTCGCTCTCCTCCCTATCCTGGAGCTTCCCGTATTTACAAACGCTCGACCTCTCCGATAACTTTTTCTCCGGATCGATCCCAGACTCGCTCTCCAACTTAACACGGCTAAGTCGACTCGGTCTCTCCGGCAATTCGTTCTCCGGCTCTATTCCCGGCTCAATTGGATTGCTTTCGAACCTCGAGGAGCTTTTCCTCGACAACAACCGCCTCGACGGCGCAATCCCACAGAGCTTGCACGGCCTGGCGCAGTTGAAGCGGCTTGAGCTTCAGGGGAACCTGCTCGGCGGCGAGTTTCCCGAATTGGGTTCTCTCCAGAACCTCTACTACTTGGACGTCAGCAACAACGCCATATCCGGTGGCGTCCCAATAAATTTTCCGCCATCTCTGCTCCAAATCTCCATGCGAAACAACAGCTTAGAAGGAAGCATCCCGGAAAACATCAAACAGTTGGGCTTTTTGCAAGTGCTCGACCTGAGTCACAACCAACTCGGCGGCGCCGTCCCTGCCCACCTCTTCAACCACCTGTCGCTACAGCAGCTCACTCTTTCCTTCAACCAATTCACGTCCGTACAATCCCCAGTTTCTCTTTCTCCGAGCACCCATAGCGAGCTCATTGCCCTAGACCTAAGCAACAACGACCTCAGGGGGATGCTGCCGCCGTTTATGGCGGCGATGCCGAAGCTCTCGGCTTTGACGCTGGAGAACAATAAGTTTACAGGTATGATTCCGACCCAGTACGCATTCAAAGTGGCGGTGCCCGGGTCCGGAGTCTCGGCGTTCGAGAGGCTCCTGCTGAGTGGGAACTACTTGTTCGGACCCATACCGGGTCCGCTGCTGCGGTTAAAACCCGGTTCGGCGAATGTGGGTTTGGGGGATAACTGCCTGTACAGGTGTCCGAGAGTTTTTTTCTTTTGTCAAGGTGGCGATCAGAAATCGTTTCATGAGTGTAGAAGCTTAGGCCCGATGATCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8

Protein Analysis

430

Amino Acids

46.4

Weight (kDa)

5.06

Isoelectric Point (pI)

38.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	108 - 217	6.1e-08	Leucine-rich repeat region
LRR_8	PF13855	162 - 199	1.5e-06	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66330
fragaria_vesca	FvH4_1g12241
malus_domestica	MD02G1136300.v1.1 MD08G1048400.v1.1 MD15G1250000.v1.1
prunus_persica	Prupe.2G102000_v2.0.a1 Prupe.7G164300_v2.0.a1
pyrus_communis	pycom15g22030
rosa_chinensis	RchiOBHm_Chr2g0100031
rosa_laevigata	RLG00000016931
rosa_multiflora	Rmu_co8338191.1_g000001 Rmu_sc0001616.1_g000058 Rmu_sc0006323.1_g000007
rosa_roxburghii	Rroxscaffold_2G00142620
rosa_rugosa	Rorug02G0084800
rosa_samantha	Rh2AG133900 Rh2BG137600 Rh2CG139000 Rh2DG139300 Rh4BG311000
rosa_wichuraiana	Rw2G010440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	651
AbsI	CCTCGAGG	1 cut(s)	494
Acc36I	ACCTGC	2 cut(s)	255, 600
AccB1I	GGYRCC	2 cut(s)	809, 1069
AccI	GTMKAC	1 cut(s)	427
AccIII	TCCGGA	2 cut(s)	383, 1079
AclWI	GGATC	3 cut(s)	385, 394, 1276
AcsI	RAATTY	1 cut(s)	685
AcuI	CTGAAG	2 cut(s)	196, 566
AcyI	GRCGYC	3 cut(s)	648, 675, 810
AfaI	GTAC	3 cut(s)	885, 1052, 1202
AfiI	CCNNNNNNNGG	6 cut(s)	273, 298, 333, 617, 1079, 1139
AgeI	ACCGGT	1 cut(s)	219
AgsI	TTSAA	7 cut(s)	11, 110, 127, 568, 832, 868, 1060
AjiI	CACGTC	1 cut(s)	879
AjnI	CCWGG	4 cut(s)	176, 297, 332, 555
AjuI	GAANNNNNNNTTGG	2 cut(s)	1166, 1198
Alw21I	GWGCWC	4 cut(s)	288, 783, 914, 927
Alw26I	GTCTC	3 cut(s)	30, 440, 1090
AlwI	GGATC	3 cut(s)	385, 394, 1276
Ama87I	CYCGRG	2 cut(s)	494, 1073
Aor13HI	TCCGGA	2 cut(s)	383, 1079
AoxI	GGCC	4 cut(s)	99, 300, 553, 1273
ApeKI	GCWGC	6 cut(s)	59, 240, 850, 967, 1147, 1150
ApoI	RAATTY	1 cut(s)	685
AsiGI	ACCGGT	1 cut(s)	219
AspLEI	GCGC	3 cut(s)	533, 562, 812
AspS9I	GGNCC	5 cut(s)	99, 1077, 1130, 1142, 1274
AsuC2I	CCSGG	7 cut(s)	159, 468, 746, 1074, 1075, 1140, 1164
AsuHPI	GGTGA	4 cut(s)	178, 217, 271, 280
AsuII	TTCGAA	1 cut(s)	488
AvaI	CYCGRG	2 cut(s)	494, 1073
AvaII	GGWCC	3 cut(s)	1077, 1130, 1142
AxyI	CCTNAGG	1 cut(s)	956
BaeGI	GKGCMC	1 cut(s)	1074
BanI	GGYRCC	2 cut(s)	809, 1069
BanII	GRGCYC	2 cut(s)	288, 927
BauI	CACGAG	1 cut(s)	163
Bbv12I	GWGCWC	4 cut(s)	288, 783, 914, 927
BbvI	GCAGC	6 cut(s)	46, 227, 862, 954, 1134, 1137
BccI	CCATC	1 cut(s)	703
BceAI	ACGGC	5 cut(s)	434, 544, 568, 797, 958
BcgI	CGANNNNNNTGC	2 cut(s)	911, 945
BciT130I	CCWGG	4 cut(s)	178, 299, 334, 557
BcnI	CCSGG	7 cut(s)	159, 468, 746, 1074, 1075, 1140, 1164
BcoDI	GTCTC	3 cut(s)	30, 440, 1090
BfaI	CTAG	1 cut(s)	935
BfmI	CTRYAG	1 cut(s)	845
BfoI	RGCGCY	1 cut(s)	813
BfuAI	ACCTGC	2 cut(s)	255, 600
BglI	GCCNNNNNGGC	1 cut(s)	528
Bme18I	GGWCC	3 cut(s)	1077, 1130, 1142
BmeT110I	CYCGRG	2 cut(s)	494, 1073
BmgBI	CACGTC	1 cut(s)	879
BmgT120I	GGNCC	5 cut(s)	99, 1077, 1130, 1142, 1274
BmiI	GGNNCC	7 cut(s)	590, 811, 1071, 1078, 1103, 1132, 1143
BmrI	ACTGGG	2 cut(s)	887, 1042
BmsI	GCATC	3 cut(s)	750, 954, 978
BmuI	ACTGGG	2 cut(s)	887, 1042
BoxI	GACNNNNGTC	1 cut(s)	789
BpmI	CTGGAG	3 cut(s)	355, 611, 1034
Bpu10I	CCTNAGC	2 cut(s)	941, 1270
Bpu14I	TTCGAA	1 cut(s)	488
BpuEI	CTTGAG	2 cut(s)	101, 596
BpuMI	CCSGG	7 cut(s)	159, 468, 746, 1074, 1075, 1140, 1164
Bsa29I	ATCGAT	1 cut(s)	389
BsaHI	GRCGYC	3 cut(s)	648, 675, 810
BsaI	GGTCTC	1 cut(s)	440
BsaJI	CCNNGG	2 cut(s)	177, 1073
BsaWI	WCCGGW	4 cut(s)	219, 383, 668, 1079
BsaXI	ACNNNNNCTCC	4 cut(s)	365, 389, 395, 419
Bsc4I	CCNNNNNNNGG	6 cut(s)	273, 298, 333, 617, 1079, 1139
Bse118I	RCCGGY	3 cut(s)	219, 273, 302
Bse1I	ACTGG	2 cut(s)	893, 1048
Bse21I	CCTNAGG	1 cut(s)	956
Bse3DI	GCAATG	1 cut(s)	927
BseAI	TCCGGA	2 cut(s)	383, 1079
BseBI	CCWGG	4 cut(s)	178, 299, 334, 557
BseCI	ATCGAT	1 cut(s)	389
BseDI	CCNNGG	2 cut(s)	177, 1073
BseGI	GGATG	2 cut(s)	741, 969
BseLI	CCNNNNNNNGG	6 cut(s)	273, 298, 333, 617, 1079, 1139
BseMI	GCAATG	1 cut(s)	927
BseMII	CTCAG	4 cut(s)	162, 779, 970, 1100
BseNI	ACTGG	2 cut(s)	893, 1048
BseRI	GAGGAG	3 cut(s)	250, 313, 512
BseSI	GKGCMC	1 cut(s)	1074
BseXI	GCAGC	6 cut(s)	46, 227, 862, 954, 1134, 1137
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	4 cut(s)	101, 302, 555, 1275
BshNI	GGYRCC	2 cut(s)	809, 1069
BshTI	ACCGGT	1 cut(s)	219
BshVI	ATCGAT	1 cut(s)	389
BsiEI	CGRYCG	1 cut(s)	220
BsiHKAI	GWGCWC	4 cut(s)	288, 783, 914, 927
BsiHKCI	CYCGRG	2 cut(s)	494, 1073
BslFI	GGGAC	3 cut(s)	194, 662, 800
BslI	CCNNNNNNNGG	6 cut(s)	273, 298, 333, 617, 1079, 1139
BsmAI	GTCTC	3 cut(s)	30, 440, 1090
BsmFI	GGGAC	3 cut(s)	194, 662, 800
BsmI	GAATGC	1 cut(s)	1055
BsnI	GGCC	4 cut(s)	101, 302, 555, 1275
Bso31I	GGTCTC	1 cut(s)	440
BsoBI	CYCGRG	2 cut(s)	494, 1073
Bsp119I	TTCGAA	1 cut(s)	488
Bsp1286I	GDGCHC	5 cut(s)	288, 783, 914, 927, 1074
Bsp13I	TCCGGA	2 cut(s)	383, 1079
Bsp1407I	TGTACA	1 cut(s)	1200
Bsp143I	GATC	4 cut(s)	386, 390, 1240, 1281
BspANI	GGCC	4 cut(s)	101, 302, 555, 1275
BspCNI	CTCAG	4 cut(s)	161, 780, 969, 1101
BspDI	ATCGAT	1 cut(s)	389
BspEI	TCCGGA	2 cut(s)	383, 1079
BspHI	TCATGA	1 cut(s)	1255
BspLI	GGNNCC	7 cut(s)	590, 811, 1071, 1078, 1103, 1132, 1143
BspMI	ACCTGC	2 cut(s)	255, 600
BspPI	GGATC	3 cut(s)	385, 394, 1276
BspT104I	TTCGAA	1 cut(s)	488
BspT107I	GGYRCC	2 cut(s)	809, 1069
BspTNI	GGTCTC	1 cut(s)	440
BsrDI	GCAATG	1 cut(s)	927
BsrFI	RCCGGY	3 cut(s)	219, 273, 302
BsrGI	TGTACA	1 cut(s)	1200
BsrI	ACTGG	2 cut(s)	893, 1048
BssAI	RCCGGY	3 cut(s)	219, 273, 302
BssECI	CCNNGG	2 cut(s)	177, 1073
BssMI	GATC	4 cut(s)	386, 390, 1240, 1281
BssNI	GRCGYC	3 cut(s)	648, 675, 810
BssSI	CACGAG	1 cut(s)	163
Bst2BI	CACGAG	1 cut(s)	163
Bst2UI	CCWGG	4 cut(s)	178, 299, 334, 557
Bst4CI	ACNGT	1 cut(s)	762
Bst6I	CTCTTC	2 cut(s)	215, 833
BstACI	GRCGYC	3 cut(s)	648, 675, 810
BstAUI	TGTACA	1 cut(s)	1200
BstBI	TTCGAA	1 cut(s)	488
BstC8I	GCNNGC	5 cut(s)	271, 275, 317, 548, 923
BstDEI	CTNAG	8 cut(s)	148, 422, 730, 788, 941, 956, 1109, 1270
BstENI	CCTNNNNNAGG	1 cut(s)	296
BstF5I	GGATG	2 cut(s)	741, 969
BstH2I	RGCGCY	1 cut(s)	813
BstHHI	GCGC	3 cut(s)	533, 562, 812
BstKTI	GATC	4 cut(s)	389, 393, 1243, 1284
BstMAI	GTCTC	3 cut(s)	30, 440, 1090
BstMBI	GATC	4 cut(s)	386, 390, 1240, 1281
BstMCI	CGRYCG	1 cut(s)	220
BstNI	CCWGG	4 cut(s)	178, 299, 334, 557
BstPAI	GACNNNNGTC	1 cut(s)	789
BstSFI	CTRYAG	1 cut(s)	845
BstSLI	GKGCMC	1 cut(s)	1074
BstV1I	GCAGC	6 cut(s)	46, 227, 862, 954, 1134, 1137
Bsu15I	ATCGAT	1 cut(s)	389
Bsu36I	CCTNAGG	1 cut(s)	956
BsuRI	GGCC	4 cut(s)	101, 302, 555, 1275
BsuTUI	ATCGAT	1 cut(s)	389
BtgZI	GCGATG	1 cut(s)	1001
BtrI	CACGTC	1 cut(s)	879
BtsCI	GGATG	2 cut(s)	741, 969
BtsIMutI	CAGTG	1 cut(s)	156
BveI	ACCTGC	2 cut(s)	255, 600
Cac8I	GCNNGC	5 cut(s)	271, 275, 317, 548, 923
CciI	TCATGA	1 cut(s)	1255
CfoI	GCGC	3 cut(s)	533, 562, 812
Cfr10I	RCCGGY	3 cut(s)	219, 273, 302
Cfr13I	GGNCC	5 cut(s)	99, 1077, 1130, 1142, 1274
Cfr9I	CCCGGG	1 cut(s)	1073
ClaI	ATCGAT	1 cut(s)	389
CseI	GACGC	3 cut(s)	157, 664, 1018
Csp6I	GTAC	3 cut(s)	884, 1051, 1201
CspAI	ACCGGT	1 cut(s)	219
CspCI	CAANNNNNGTGG	2 cut(s)	528, 563
CviAII	CATG	3 cut(s)	715, 1256, 1287
CviQI	GTAC	3 cut(s)	884, 1051, 1201
DdeI	CTNAG	8 cut(s)	148, 422, 730, 788, 941, 956, 1109, 1270
DinI	GGCGCC	1 cut(s)	811
DpnI	GATC	4 cut(s)	388, 392, 1242, 1283
DpnII	GATC	4 cut(s)	386, 390, 1240, 1281
Eam1104I	CTCTTC	2 cut(s)	215, 833
EarI	CTCTTC	2 cut(s)	215, 833
EciI	GGCGGA	2 cut(s)	256, 681
Ecl136II	GAGCTC	2 cut(s)	286, 925
Eco24I	GRGCYC	2 cut(s)	288, 927
Eco31I	GGTCTC	1 cut(s)	440
Eco47I	GGWCC	3 cut(s)	1077, 1130, 1142
Eco53kI	GAGCTC	2 cut(s)	286, 925
Eco57I	CTGAAG	2 cut(s)	196, 566
Eco81I	CCTNAGG	1 cut(s)	956
Eco88I	CYCGRG	2 cut(s)	494, 1073
EcoICRI	GAGCTC	2 cut(s)	286, 925
EcoNI	CCTNNNNNAGG	1 cut(s)	296
EcoRII	CCWGG	4 cut(s)	176, 297, 332, 555
EcoT38I	GRGCYC	2 cut(s)	288, 927
EgeI	GGCGCC	1 cut(s)	811
EheI	GGCGCC	1 cut(s)	811
FaeI	CATG	3 cut(s)	718, 1259, 1290
FaiI	YATR	8 cut(s)	665, 716, 918, 980, 1037, 1136, 1257, 1288
FaqI	GGGAC	3 cut(s)	194, 662, 800
FatI	CATG	3 cut(s)	714, 1255, 1286
FblI	GTMKAC	1 cut(s)	427
FokI	GGATG	2 cut(s)	728, 976
FriOI	GRGCYC	2 cut(s)	288, 927
FspBI	CTAG	1 cut(s)	935
GlaI	GCGC	3 cut(s)	532, 561, 811
GsuI	CTGGAG	3 cut(s)	355, 611, 1034
HaeII	RGCGCY	1 cut(s)	813
HaeIII	GGCC	4 cut(s)	101, 302, 555, 1275
HgaI	GACGC	3 cut(s)	157, 664, 1018
HhaI	GCGC	3 cut(s)	533, 562, 812
Hin1I	GRCGYC	3 cut(s)	648, 675, 810
Hin1II	CATG	3 cut(s)	718, 1259, 1290
Hin6I	GCGC	3 cut(s)	531, 560, 810
HinP1I	GCGC	3 cut(s)	531, 560, 810
HincII	GTYRAC	2 cut(s)	172, 428
HindII	GTYRAC	2 cut(s)	172, 428
HindIII	AAGCTT	1 cut(s)	1267
HinfI	GANTC	8 cut(s)	83, 146, 154, 398, 429, 790, 1039, 1083
HphI	GGTGA	4 cut(s)	178, 217, 271, 280
Hpy166II	GTNNAC	4 cut(s)	172, 225, 428, 1029
Hpy188I	TCNGA	6 cut(s)	370, 909, 1044, 1130, 1212, 1245
Hpy188III	TCNNGA	7 cut(s)	127, 384, 611, 628, 1080, 1096, 1256
Hpy8I	GTNNAC	4 cut(s)	172, 225, 428, 1029
Hpy99I	CGWCG	1 cut(s)	530
HpyAV	CCTTC	2 cut(s)	728, 874
HpyCH4III	ACNGT	1 cut(s)	762
HpyCH4IV	ACGT	2 cut(s)	648, 878
HpyCH4V	TGCA	4 cut(s)	62, 70, 550, 775
HpyF3I	CTNAG	8 cut(s)	148, 422, 730, 788, 941, 956, 1109, 1270
HpySE526I	ACGT	2 cut(s)	648, 878
Hsp92I	GRCGYC	3 cut(s)	648, 675, 810
Hsp92II	CATG	3 cut(s)	718, 1259, 1290
HspAI	GCGC	3 cut(s)	531, 560, 810
KasI	GGCGCC	1 cut(s)	809
Kpn2I	TCCGGA	2 cut(s)	383, 1079
KroI	GCCGGC	1 cut(s)	273
KroNI	GCCGGC	1 cut(s)	275
Kzo9I	GATC	4 cut(s)	386, 390, 1240, 1281
LmnI	GCTCC	4 cut(s)	336, 499, 708, 1107
Lsp1109I	GCAGC	6 cut(s)	46, 227, 862, 954, 1134, 1137
LweI	GCATC	3 cut(s)	750, 954, 978
MaeI	CTAG	1 cut(s)	935
MaeII	ACGT	2 cut(s)	648, 878
MaeIII	GTNAC	3 cut(s)	151, 305, 791
MalI	GATC	4 cut(s)	388, 392, 1242, 1283
MboI	GATC	4 cut(s)	386, 390, 1240, 1281
MboII	GAAGA	2 cut(s)	202, 820
MfeI	CAATTG	1 cut(s)	474
MhlI	GDGCHC	5 cut(s)	288, 783, 914, 927, 1074
MluCI	AATT	7 cut(s)	19, 51, 445, 474, 614, 685, 872
Mly113I	GGCGCC	1 cut(s)	810
MlyI	GAGTC	6 cut(s)	140, 148, 392, 423, 799, 1092
MmeI	TCCRAC	2 cut(s)	432, 1067
MroI	TCCGGA	2 cut(s)	383, 1079
MroNI	GCCGGC	1 cut(s)	273
MseI	TTAA	2 cut(s)	413, 1157
MspA1I	CMGCKG	3 cut(s)	59, 240, 1147
MunI	CAATTG	1 cut(s)	474
Mva1269I	GAATGC	1 cut(s)	1055
MvaI	CCWGG	4 cut(s)	178, 299, 334, 557
NaeI	GCCGGC	1 cut(s)	275
NarI	GGCGCC	1 cut(s)	810
NciI	CCSGG	7 cut(s)	159, 468, 746, 1074, 1075, 1140, 1164
NdeII	GATC	4 cut(s)	386, 390, 1240, 1281
NgoMIV	GCCGGC	1 cut(s)	273
NlaIII	CATG	3 cut(s)	718, 1259, 1290
NlaIV	GGNNCC	7 cut(s)	590, 811, 1071, 1078, 1103, 1132, 1143
NmeAIII	GCCGAG	4 cut(s)	576, 783, 980, 1067
NmuCI	GTSAC	2 cut(s)	151, 791
NspV	TTCGAA	1 cut(s)	488
PaeR7I	CTCGAG	1 cut(s)	494
PagI	TCATGA	1 cut(s)	1255
PcsI	WCGNNNNNNNCGW	4 cut(s)	198, 366, 424, 810
PctI	GAATGC	1 cut(s)	1055
PdiI	GCCGGC	1 cut(s)	275
PfeI	GAWTC	2 cut(s)	83, 1039
PflFI	GACNNNGTC	1 cut(s)	432
PfoI	TCCNGGA	2 cut(s)	332, 744
PinAI	ACCGGT	1 cut(s)	219
PleI	GAGTC	6 cut(s)	140, 148, 392, 423, 798, 1091
PluTI	GGCGCC	1 cut(s)	813
PpsI	GAGTC	6 cut(s)	140, 148, 392, 423, 798, 1091
PshAI	GACNNNNGTC	1 cut(s)	789
Psp124BI	GAGCTC	2 cut(s)	288, 927
Psp6I	CCWGG	4 cut(s)	176, 297, 332, 555
PspGI	CCWGG	4 cut(s)	176, 297, 332, 555
PspN4I	GGNNCC	7 cut(s)	590, 811, 1071, 1078, 1103, 1132, 1143
PspPI	GGNCC	5 cut(s)	99, 1077, 1130, 1142, 1274
PspXI	VCTCGAGB	1 cut(s)	494
PsrI	GAACNNNNNNTAC	2 cut(s)	623, 655
PsyI	GACNNNGTC	1 cut(s)	432
PvuII	CAGCTG	1 cut(s)	59
RsaI	GTAC	3 cut(s)	885, 1052, 1202
RsaNI	GTAC	3 cut(s)	884, 1051, 1201
SacI	GAGCTC	2 cut(s)	288, 927
SalI	GTCGAC	1 cut(s)	426
SaqAI	TTAA	2 cut(s)	413, 1157
Sau3AI	GATC	4 cut(s)	386, 390, 1240, 1281
Sau96I	GGNCC	5 cut(s)	99, 1077, 1130, 1142, 1274
SchI	GAGTC	6 cut(s)	140, 148, 392, 423, 799, 1092
SduI	GDGCHC	5 cut(s)	288, 783, 914, 927, 1074
SfaNI	GCATC	3 cut(s)	750, 954, 978
SfcI	CTRYAG	1 cut(s)	845
SfoI	GGCGCC	1 cut(s)	811
Sfr274I	CTCGAG	1 cut(s)	494
SfuI	TTCGAA	1 cut(s)	488
SinI	GGWCC	3 cut(s)	1077, 1130, 1142
SlaI	CTCGAG	1 cut(s)	494
SmaI	CCCGGG	1 cut(s)	1075
SmlI	CTYRAG	3 cut(s)	116, 494, 575
SmoI	CTYRAG	3 cut(s)	116, 494, 575
Sse9I	AATT	7 cut(s)	19, 51, 445, 474, 614, 685, 872
SspDI	GGCGCC	1 cut(s)	809
SspMI	CTAG	1 cut(s)	935
SstI	GAGCTC	2 cut(s)	288, 927
TaaI	ACNGT	1 cut(s)	762
TaiI	ACGT	2 cut(s)	651, 881
TaqII	GACCGA	1 cut(s)	422
TasI	AATT	7 cut(s)	19, 51, 445, 474, 614, 685, 872
TatI	WGTACW	1 cut(s)	1200
TauI	GCSGC	5 cut(s)	574, 603, 810, 973, 986
TfiI	GAWTC	2 cut(s)	83, 1039
Tru1I	TTAA	2 cut(s)	413, 1157
Tru9I	TTAA	2 cut(s)	413, 1157
TscAI	CASTG	1 cut(s)	156
TseFI	GTSAC	2 cut(s)	151, 791
TseI	GCWGC	6 cut(s)	59, 240, 850, 967, 1147, 1150
Tsp45I	GTSAC	2 cut(s)	151, 791
TspDTI	ATGAA	1 cut(s)	1244
TspGWI	ACGGA	1 cut(s)	871
TspMI	CCCGGG	1 cut(s)	1073
TspRI	CASTG	1 cut(s)	156
Tth111I	GACNNNGTC	1 cut(s)	432
VpaK11BI	GGWCC	3 cut(s)	1077, 1130, 1142
XagI	CCTNNNNNAGG	1 cut(s)	296
XapI	RAATTY	1 cut(s)	685
XhoI	CTCGAG	1 cut(s)	494
XmaI	CCCGGG	1 cut(s)	1073
XmiI	GTMKAC	1 cut(s)	427
XspI	CTAG	1 cut(s)	935
ZraI	GACGTC	1 cut(s)	649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.