MD02G1136300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010221
Physical Location & Seq
Reverse (-)
11368733 .. 11375554
6822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1136300.v1.1.491

Sequence Viewer

Length: 3093 bp
ATGGGTGTCTCCATTTCCGATTTCTCCCTATTTTTCCTGATCATATTCGTAATTTCATCCGCAAGTCTGCAGCTTGGAGCCGAATCCAAGACTTTCTGGGCCGACATTGAAGCTCTCAAGGAGTTCAAGAACTCACTCGACCCCGGCTCAGTGCGTCCGGGCTCGTGCGTCAGCTCCTGGGACTTCAACCTCGACCCATGCGACAACCTCTTCAGCGACCGGTTCACCTGCGGCTTCCGCTGCGACAAGGTTGACTCCTCCTCGTCAGTCAGCAGGCTCACCGAGCTCACCCTCGACCAGGCCGGCTACTCCGGCTCGCTCTCCTCCCTCTCCTGGAACTTCCCGTATTTGCAAACACTCGACCTCTCCAACAACTTCTTCTCCGGGTCGATCCCCGAGTCGCTCTCCAACTTAACAGGGCTGATTCGACTCGGTCTCTCCGGAAACTCGTTCTCCGGTGCAATTCCCGACTCAATTGGATCGCTTTCGAACCTCGTGGAGCTTTTTCTCGACAACAACCGCCTCAACGGCGCAATCCCATCGAGCTTGAACGGCCTGGCGGGGTTGCAGCGGCTCGAGCTCCAAGGGAACCTGCTCAGCGGCGAGTTTCCCGAGTTGGGTTCTCTTCAGAACCTCAGCTACTTAGACGCCAGCAACAACGCCATCTCCGGAGGTGTCCCACTAAATTTCCCGACCTCTCTGCTCCAAATCTCCATGCGAAACAACAGCCTAGTAGGAAACATCCCGGAAAACATCAAACAGTTGGGTTTTCTGCAAGTGCTCGACCTGAGTCACAACCAACTCGGCGGTGCCGTCCCCGCCCACCTCTTCAACCACCTGTCGCTACAGCAGCTCACTCTCTCCTTCAACCAATTCACGTCCGTAAAGCCCCCGGTTTCTCCTTCTCCGGGCGCCCAGAGCGCGCTCATTGCACTAGACCTCAGCAACAACGAGCTCAGCGGAATGCTACCGCTGTTCATGGCGGCGATGCGGAAGCTCTCAGCATTGACGCTGGAGAACAATAAGTTCACGGGTATGATTCCGACCCAGTACGCGCTCAAAGTGGCGGTGCCCGGATCCGGAGTCTCGAAGCTCGAGAGGCTCCTGCTGGGTGGGAATTACTTGTCCGGACCCATCCCGCGTCCTCTGCTCGGGTTAGAACCCGGTTCGGCGAATGTGGTTTTGGGCAATAATTGCCTGTACAGGTGTCCCAGGATTTACCATGTTTGTAAAGGTGGCCATCAGAAATCGTATCATAAGTACAGACAAGCACTTCTACATTTCAAGCACAACATCGTTTCTGACCCTTCAAAACTGCTGCATTCGTGGTCAATCTCCTCCGATTGCTGCTCCGCTTGGGAAGGCGTCGCGTGTGAATCCTCCGGCAGAGTTGTAAACGTCTCACGCCCGGGGCTTGTTTCCGGCAATGACTTCCTGGTCGACACGTATATGTCCGGTACTTTGTCCCCTTATCTTGGAAACTTATCATTTCTTCAACTTCTTGACCTCAGTAATCTCAAGGACTTGAAGGGTCCAATACCACAAGAATTTGGGAAGCTATCTCACCTCACTCATCTCTTCCTTGATTCAAACATGCTCATTGGCTCGATACCAACAGCATTTCGCTATCTTTTTCGATTGGAGAAGCTCTATCTGGGTAACAATTACATATCTGGTGTTGTTCCCTCCTCTGTTTTTGGATCTTTGAGCTCTCTCTCGGAATTAGGCCTATCCGGAAATCGGTTGTCGGGCCAAATTCCAACTTCAATAGGAAAGTTGGTTTTACTAAGCAAGCTTGATCTCCATGCAAACAATATCTCCGGAAGCATTCCAATCTCCATTGGCAAGCTTAAGAGCTTGACATATCTTGATTTATCCGAAAATCAGATAAGTGGAAGACTGCCTCAGTCCATTGGTGCACTTTCGCAATTAGTCCTGCTCTATCTCAATCATAATCAGCTCACTGGAAGCATTCCTTCTTCGATTTCCGGGCTTAATTCTCTGCGGTTTTGTCAGTTATCCCAAAACAAGCTCGCAGGTGCTTTGCCAGCATCACTAGGCCAGCTCCCAAAGATCGAAAGGCTCATTTTCGAGAACAACAAACTTTCAGGAAAACTACCAGCAGCCATTGGCCACCTTGCAACTCTCACTGACATTTTCTTCTCCAACAATCGTTTTACAGGCAAGATTCCTTCAAGTTTTTCCAATTTACATAACCTACAAACACTAGATTTGTCGAGAAATCGACTCATTGGTCAAATTCCTCCTCAGCTTGCAAAATTACTTAGTCTAGACACTCTGGATCTTTCATTCAATCCACTGGGATTGGTTAGTGTACCAAGCTTTTTTGCAAGATTGAAACTTTTTCGGCTATTGTTGGCGAAAACCGGCATTGAAGGGCTGCTTCCGAATTGGTTATCTTCATCATCTGTCTCCATACTTGACTTATCAAGCAATGCCTTGACAGGCAAGCTGCCTCATTGGATAGGCAACATGACTAGCCTTTCATTTCTGAACTTATCAAACAATGGTTTCCATTCATCAATCCCAGCAGAATTCAGAAACCTTGCACTTCTGATGGATCTTGATCTGCATTCCAACAAGTTCTCCGGCCACCTGAGCTCGATATTTTCGAAACAATCCCAAAACCCTCTTGGACAATTCAACTCCCTTGATCTTTCCAACAACATGTTCACCGGCCCGATTGATGAGGACATCGGAGAAATTCCCACAATGGCAGCCATCAAAAGTCTAGTTTTATCAAACAACCCCTTGAGAGGAAGCATACCGAAGTCATTGGGAAAACTGAGTGATTTGCAGGTTCTAAAGCTTGCGCATAATCGGCTTTCGGGAAGGATTCAATCAGAGCTTGGAGATGCCAGAAAACTTACTACACTTTCTCTTTCAAGTAATAACTTGAGTGGAAATATTCCAAAGGAGGTGCTGAATTTGAAAGACCTTAAAGAATTTGATGTTTCTAGGAACCATTTGAGTGGGAAAATTCCTCCTCACAAAGCCATTATTCCCGTTTCCGGTTTCATAGATAACCCTGGCTTGTGTGGAGTTCCACTTCCTCCTTGTAATCACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

1031

Amino Acids

111.14

Weight (kDa)

8.69

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 109 - 218 2e-06 Leucine-rich repeat region
LRR_8 PF13855 115 - 175 6.1e-07 Leucine rich repeat
LRR_8 PF13855 141 - 199 2.3e-06 Leucine rich repeat
LRRNT_2 PF08263 422 - 458 1.4e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 546 - 606 3.5e-08 Leucine rich repeat
LRR_8 PF13855 571 - 630 5e-07 Leucine rich repeat
LRR_14 PF23598 587 - 669 1.9e-10 Leucine-rich repeat region
LRR_8 PF13855 596 - 654 4.1e-08 Leucine rich repeat
LRR_8 PF13855 619 - 678 3e-07 Leucine rich repeat
LRR_8 PF13855 716 - 774 3.3e-07 Leucine rich repeat
LRR_4 PF12799 716 - 755 9.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 790 - 844 9.6e-06 Leucine rich repeat
LRR_8 PF13855 810 - 869 1e-05 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 236, 2027
AasI GACNNNNNNGTC 2 cut(s) 1436, 2253
Acc16I TGCGCA 1 cut(s) 2835
Acc36I ACCTGC 4 cut(s) 236, 600, 2027, 2809
AccB1I GGYRCC 3 cut(s) 809, 911, 1069
AccI GTMKAC 1 cut(s) 1440
AccII CGCG 4 cut(s) 923, 1055, 1141, 1370
AccIII TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
AclWI GGATC 7 cut(s) 385, 487, 1071, 1084, 1708, 2310, 2589
AcoI YGGCCR 3 cut(s) 1237, 2131, 2611
AcsI RAATTY 9 cut(s) 685, 1547, 1755, 2259, 2555, 2724, 2947, 2966, 3000
AcuI CTGAAG 2 cut(s) 196, 611
AcyI GRCGYC 3 cut(s) 648, 912, 1365
AfaI GTAC 5 cut(s) 1052, 1202, 1262, 1459, 2337
AflII CTTAAG 1 cut(s) 1850
AflIII ACRYGT 2 cut(s) 1443, 2688
AgeI ACCGGT 1 cut(s) 219
AjiI CACGTC 1 cut(s) 879
AjnI CCWGG 7 cut(s) 176, 297, 332, 555, 1211, 1434, 3049
AjuI GAANNNNNNNTTGG 6 cut(s) 362, 394, 1166, 1198, 2675, 2707
Alw21I GWGCWC 7 cut(s) 288, 582, 783, 957, 1712, 1921, 2624
Alw26I GTCTC 5 cut(s) 13, 440, 1090, 1405, 2437
Alw44I GTGCAC 1 cut(s) 1917
AlwI GGATC 7 cut(s) 385, 487, 1071, 1084, 1708, 2310, 2589
AlwNI CAGNNNCTG 1 cut(s) 177
Ama87I CYCGRG 6 cut(s) 395, 575, 611, 1094, 1151, 1408
Aor13HI TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
ApaLI GTGCAC 1 cut(s) 1917
ApoI RAATTY 9 cut(s) 685, 1547, 1755, 2259, 2555, 2724, 2947, 2966, 3000
AsiGI ACCGGT 1 cut(s) 219
AspLEI GCGC 6 cut(s) 533, 914, 923, 925, 1057, 2836
AspS9I GGNCC 5 cut(s) 99, 1130, 1532, 1750, 2699
AsuHPI GGTGA 5 cut(s) 217, 271, 280, 1556, 2686
AsuII TTCGAA 2 cut(s) 488, 2633
AvaI CYCGRG 6 cut(s) 395, 575, 611, 1094, 1151, 1408
AvaII GGWCC 2 cut(s) 1130, 1532
BaeGI GKGCMC 2 cut(s) 1074, 1921
BalI TGGCCA 2 cut(s) 1239, 2133
BamHI GGATCC 1 cut(s) 1076
BanI GGYRCC 3 cut(s) 809, 911, 1069
BanII GRGCYC 6 cut(s) 164, 288, 582, 957, 1712, 2624
BauI CACGAG 2 cut(s) 163, 494
BbsI GAAGAC 1 cut(s) 1903
Bbv12I GWGCWC 7 cut(s) 288, 582, 783, 957, 1712, 1921, 2624
BbvCI CCTCAGC 3 cut(s) 635, 941, 2268
BccI CCATC 6 cut(s) 547, 671, 1142, 1248, 2572, 2750
BceAI ACGGC 3 cut(s) 544, 568, 797
BcgI CGANNNNNNTGC 4 cut(s) 522, 556, 682, 716
BciT130I CCWGG 7 cut(s) 178, 299, 334, 557, 1213, 1436, 3051
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 5 cut(s) 13, 440, 1090, 1405, 2437
BfaI CTAG 8 cut(s) 731, 935, 2057, 2228, 2291, 2499, 2753, 2979
BfmI CTRYAG 2 cut(s) 68, 845
BfoI RGCGCY 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 1850
BfuAI ACCTGC 4 cut(s) 236, 600, 2027, 2809
BglI GCCNNNNNGGC 1 cut(s) 528
BlpI GCTNAGC 2 cut(s) 596, 956
Bme18I GGWCC 2 cut(s) 1130, 1532
BmeT110I CYCGRG 6 cut(s) 395, 575, 611, 1094, 1151, 1408
BmgBI CACGTC 1 cut(s) 879
BmgT120I GGNCC 5 cut(s) 99, 1130, 1532, 1750, 2699
BmrI ACTGGG 2 cut(s) 1042, 2330
BmsI GCATC 3 cut(s) 978, 2060, 2866
BmuI ACTGGG 2 cut(s) 1042, 2330
BoxI GACNNNNGTC 1 cut(s) 789
BpiI GAAGAC 1 cut(s) 1903
BplI GAGNNNNNCTC 2 cut(s) 389, 421
BpmI CTGGAG 1 cut(s) 1034
Bpu10I CCTNAGC 4 cut(s) 635, 941, 2268, 2618
Bpu1102I GCTNAGC 2 cut(s) 596, 956
Bpu14I TTCGAA 2 cut(s) 488, 2633
BpuEI CTTGAG 4 cut(s) 101, 1502, 2794, 2938
BsaAI YACGTR 1 cut(s) 1446
BsaBI GATNNNNATC 1 cut(s) 2586
BsaHI GRCGYC 3 cut(s) 648, 912, 1365
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 8 cut(s) 142, 177, 583, 891, 1211, 1408, 1409, 3049
Bse118I RCCGGY 4 cut(s) 219, 302, 2387, 2696
Bse1I ACTGG 3 cut(s) 1048, 1969, 2325
Bse3DI GCAATG 3 cut(s) 927, 1432, 2461
Bse8I GATNNNNATC 1 cut(s) 2586
BseAI TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
BseBI CCWGG 7 cut(s) 178, 299, 334, 557, 1213, 1436, 3051
BseDI CCNNGG 8 cut(s) 142, 177, 583, 891, 1211, 1408, 1409, 3049
BseGI GGATG 3 cut(s) 56, 741, 1134
BseJI GATNNNNATC 1 cut(s) 2586
BseMI GCAATG 3 cut(s) 927, 1432, 2461
BseNI ACTGG 3 cut(s) 1048, 1969, 2325
BsePI GCGCGC 1 cut(s) 921
BseRI GAGGAG 7 cut(s) 247, 250, 313, 1327, 1678, 2256, 2997
BseSI GKGCMC 2 cut(s) 1074, 1921
BseYI CCCAGC 2 cut(s) 1108, 2548
Bsh1236I CGCG 4 cut(s) 923, 1055, 1141, 1370
Bsh1285I CGRYCG 1 cut(s) 220
BshNI GGYRCC 3 cut(s) 809, 911, 1069
BshTI ACCGGT 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 7 cut(s) 288, 582, 783, 957, 1712, 1921, 2624
BsiHKCI CYCGRG 6 cut(s) 395, 575, 611, 1094, 1151, 1408
BslFI GGGAC 5 cut(s) 194, 662, 800, 1194, 1450
BsmAI GTCTC 5 cut(s) 13, 440, 1090, 1405, 2437
BsmBI CGTCTC 1 cut(s) 1405
BsmFI GGGAC 5 cut(s) 194, 662, 800, 1194, 1450
BsmI GAATGC 5 cut(s) 969, 1321, 1827, 1971, 2593
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 6 cut(s) 395, 575, 611, 1094, 1151, 1408
Bsp119I TTCGAA 2 cut(s) 488, 2633
Bsp1286I GDGCHC 9 cut(s) 164, 288, 582, 783, 957, 1074, 1712, 1921, 2624
Bsp13I TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
Bsp1407I TGTACA 1 cut(s) 1200
Bsp1720I GCTNAGC 2 cut(s) 596, 956
BspEI TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
BspFNI CGCG 4 cut(s) 923, 1055, 1141, 1370
BspMAI CTGCAG 1 cut(s) 72
BspMI ACCTGC 4 cut(s) 236, 600, 2027, 2809
BspPI GGATC 7 cut(s) 385, 487, 1071, 1084, 1708, 2310, 2589
BspT104I TTCGAA 2 cut(s) 488, 2633
BspT107I GGYRCC 3 cut(s) 809, 911, 1069
BspTI CTTAAG 1 cut(s) 1850
BspTNI GGTCTC 1 cut(s) 440
BsrDI GCAATG 3 cut(s) 927, 1432, 2461
BsrFI RCCGGY 4 cut(s) 219, 302, 2387, 2696
BsrGI TGTACA 1 cut(s) 1200
BsrI ACTGG 3 cut(s) 1048, 1969, 2325
BssAI RCCGGY 4 cut(s) 219, 302, 2387, 2696
BssECI CCNNGG 8 cut(s) 142, 177, 583, 891, 1211, 1408, 1409, 3049
BssHII GCGCGC 1 cut(s) 921
BssNI GRCGYC 3 cut(s) 648, 912, 1365
BssSI CACGAG 2 cut(s) 163, 494
BssT1I CCWWGG 1 cut(s) 583
Bst2BI CACGAG 2 cut(s) 163, 494
Bst2UI CCWGG 7 cut(s) 178, 299, 334, 557, 1213, 1436, 3051
Bst4CI ACNGT 1 cut(s) 762
Bst6I CTCTTC 4 cut(s) 215, 630, 833, 1583
BstACI GRCGYC 3 cut(s) 648, 912, 1365
BstAFI CTTAAG 1 cut(s) 1850
BstAPI GCANNNNNTGC 1 cut(s) 1194
BstAUI TGTACA 1 cut(s) 1200
BstBAI YACGTR 1 cut(s) 1446
BstBI TTCGAA 2 cut(s) 488, 2633
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 3 cut(s) 56, 741, 1134
BstFNI CGCG 4 cut(s) 923, 1055, 1141, 1370
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 6 cut(s) 533, 914, 923, 925, 1057, 2836
BstMAI GTCTC 5 cut(s) 13, 440, 1090, 1405, 2437
BstMCI CGRYCG 1 cut(s) 220
BstNI CCWGG 7 cut(s) 178, 299, 334, 557, 1213, 1436, 3051
BstNSI RCATGY 2 cut(s) 1597, 2692
BstPAI GACNNNNGTC 1 cut(s) 789
BstSFI CTRYAG 2 cut(s) 68, 845
BstSLI GKGCMC 2 cut(s) 1074, 1921
BstUI CGCG 4 cut(s) 923, 1055, 1141, 1370
BstV2I GAAGAC 1 cut(s) 1903
BstX2I RGATCY 4 cut(s) 1076, 1700, 2302, 2581
BstXI CCANNNNNNTGG 1 cut(s) 2993
BstYI RGATCY 4 cut(s) 1076, 1700, 2302, 2581
BtgZI GCGATG 1 cut(s) 1001
BtrI CACGTC 1 cut(s) 879
BtsCI GGATG 3 cut(s) 56, 741, 1134
BtsIMutI CAGTG 4 cut(s) 156, 1962, 2148, 2318
BveI ACCTGC 4 cut(s) 236, 600, 2027, 2809
CaiI CAGNNNCTG 1 cut(s) 177
CfoI GCGC 6 cut(s) 533, 914, 923, 925, 1057, 2836
Cfr10I RCCGGY 4 cut(s) 219, 302, 2387, 2696
Cfr13I GGNCC 5 cut(s) 99, 1130, 1532, 1750, 2699
Cfr9I CCCGGG 1 cut(s) 1408
CseI GACGC 6 cut(s) 143, 157, 656, 1018, 1130, 1354
Csp6I GTAC 5 cut(s) 1051, 1201, 1261, 1458, 2336
CspAI ACCGGT 1 cut(s) 219
CspCI CAANNNNNGTGG 2 cut(s) 2307, 2342
CviAII CATG 8 cut(s) 198, 715, 979, 1223, 1594, 1805, 2494, 2689
CviQI GTAC 5 cut(s) 1051, 1201, 1261, 1458, 2336
DinI GGCGCC 1 cut(s) 913
DrdI GACNNNNNNGTC 2 cut(s) 1436, 2253
DseDI GACNNNNNNGTC 2 cut(s) 1436, 2253
EaeI YGGCCR 3 cut(s) 1237, 2131, 2611
Eam1104I CTCTTC 4 cut(s) 215, 630, 833, 1583
EarI CTCTTC 4 cut(s) 215, 630, 833, 1583
Ecl136II GAGCTC 5 cut(s) 286, 580, 955, 1710, 2622
Eco130I CCWWGG 1 cut(s) 583
Eco147I AGGCCT 1 cut(s) 1728
Eco24I GRGCYC 6 cut(s) 164, 288, 582, 957, 1712, 2624
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 1130, 1532
Eco53kI GAGCTC 5 cut(s) 286, 580, 955, 1710, 2622
Eco57I CTGAAG 2 cut(s) 196, 611
Eco88I CYCGRG 6 cut(s) 395, 575, 611, 1094, 1151, 1408
EcoICRI GAGCTC 5 cut(s) 286, 580, 955, 1710, 2622
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 2555
EcoRII CCWGG 7 cut(s) 176, 297, 332, 555, 1211, 1434, 3049
EcoT14I CCWWGG 1 cut(s) 583
EcoT38I GRGCYC 6 cut(s) 164, 288, 582, 957, 1712, 2624
EgeI GGCGCC 1 cut(s) 913
EheI GGCGCC 1 cut(s) 913
ErhI CCWWGG 1 cut(s) 583
Esp3I CGTCTC 1 cut(s) 1405
FaeI CATG 8 cut(s) 201, 718, 982, 1226, 1597, 1808, 2497, 2692
FalI AAGNNNNNCTT 4 cut(s) 1960, 1992, 2388, 2420
FaqI GGGAC 5 cut(s) 194, 662, 800, 1194, 1450
FatI CATG 8 cut(s) 197, 714, 978, 1222, 1593, 1804, 2493, 2688
FauI CCCGC 3 cut(s) 553, 826, 1146
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 1440
FokI GGATG 3 cut(s) 43, 728, 1121
FriOI GRGCYC 6 cut(s) 164, 288, 582, 957, 1712, 2624
FspBI CTAG 8 cut(s) 731, 935, 2057, 2228, 2291, 2499, 2753, 2979
FspI TGCGCA 1 cut(s) 2835
GlaI GCGC 6 cut(s) 532, 913, 922, 924, 1056, 2835
GsaI CCCAGC 2 cut(s) 1112, 2552
GsuI CTGGAG 1 cut(s) 1034
HaeII RGCGCY 1 cut(s) 915
HgaI GACGC 6 cut(s) 143, 157, 656, 1018, 1130, 1354
HhaI GCGC 6 cut(s) 533, 914, 923, 925, 1057, 2836
Hin1I GRCGYC 3 cut(s) 648, 912, 1365
Hin1II CATG 8 cut(s) 201, 718, 982, 1226, 1597, 1808, 2497, 2692
Hin6I GCGC 6 cut(s) 531, 912, 921, 923, 1055, 2834
HinP1I GCGC 6 cut(s) 531, 912, 921, 923, 1055, 2834
HincII GTYRAC 2 cut(s) 253, 1441
HindII GTYRAC 2 cut(s) 253, 1441
HindIII AAGCTT 4 cut(s) 1793, 1847, 2341, 2828
HphI GGTGA 5 cut(s) 217, 271, 280, 1556, 2686
Hpy166II GTNNAC 8 cut(s) 225, 253, 1029, 1396, 1441, 1919, 2336, 2694
Hpy8I GTNNAC 8 cut(s) 225, 253, 1029, 1396, 1441, 1919, 2336, 2694
Hpy99I CGWCG 1 cut(s) 1370
HpyAV CCTTC 9 cut(s) 874, 912, 1317, 1355, 1522, 1986, 2202, 2390, 2847
HpyCH4III ACNGT 1 cut(s) 762
HpyCH4IV ACGT 3 cut(s) 878, 1398, 1445
HpySE526I ACGT 3 cut(s) 878, 1398, 1445
Hsp92I GRCGYC 3 cut(s) 648, 912, 1365
Hsp92II CATG 8 cut(s) 201, 718, 982, 1226, 1597, 1808, 2497, 2692
HspAI GCGC 6 cut(s) 531, 912, 921, 923, 1055, 2834
KasI GGCGCC 1 cut(s) 911
Kpn2I TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 39
LmnI GCTCC 8 cut(s) 77, 179, 499, 585, 708, 1107, 1355, 2070
LweI GCATC 3 cut(s) 978, 2060, 2866
MaeI CTAG 8 cut(s) 731, 935, 2057, 2228, 2291, 2499, 2753, 2979
MaeII ACGT 3 cut(s) 878, 1398, 1445
MaeIII GTNAC 2 cut(s) 791, 1658
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 4 cut(s) 1076, 1700, 2302, 2581
MhlI GDGCHC 9 cut(s) 164, 288, 582, 783, 957, 1074, 1712, 1921, 2624
MlsI TGGCCA 2 cut(s) 1239, 2133
MluNI TGGCCA 2 cut(s) 1239, 2133
Mly113I GGCGCC 1 cut(s) 912
MlyI GAGTC 7 cut(s) 248, 407, 423, 464, 799, 1092, 2241
MmeI TCCRAC 7 cut(s) 393, 432, 1067, 1784, 2190, 2622, 2706
Mox20I TGGCCA 2 cut(s) 1239, 2133
MroI TCCGGA 6 cut(s) 440, 668, 1079, 1127, 1733, 1820
MroNI GCCGGC 1 cut(s) 302
MscI TGGCCA 2 cut(s) 1239, 2133
MseI TTAA 4 cut(s) 413, 1851, 1995, 2961
MslI CAYNNNNRTG 3 cut(s) 1034, 1448, 2991
Msp20I TGGCCA 2 cut(s) 1239, 2133
MspA1I CMGCKG 5 cut(s) 240, 571, 600, 960, 973
MspCI CTTAAG 1 cut(s) 1850
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 5 cut(s) 969, 1321, 1827, 1971, 2593
MvaI CCWGG 7 cut(s) 178, 299, 334, 557, 1213, 1436, 3051
MvnI CGCG 4 cut(s) 923, 1055, 1141, 1370
NaeI GCCGGC 1 cut(s) 304
NarI GGCGCC 1 cut(s) 912
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 8 cut(s) 201, 718, 982, 1226, 1597, 1808, 2497, 2692
NmeAIII GCCGAG 1 cut(s) 783
NmuCI GTSAC 1 cut(s) 791
NsbI TGCGCA 1 cut(s) 2835
NspI RCATGY 2 cut(s) 1597, 2692
NspV TTCGAA 2 cut(s) 488, 2633
PaeR7I CTCGAG 2 cut(s) 575, 1094
PaqCI CACCTGC 2 cut(s) 236, 2027
PauI GCGCGC 1 cut(s) 921
PceI AGGCCT 1 cut(s) 1728
PciI ACATGT 1 cut(s) 2688
PcsI WCGNNNNNNNCGW 3 cut(s) 198, 810, 2630
PctI GAATGC 5 cut(s) 969, 1321, 1827, 1971, 2593
PdiI GCCGGC 1 cut(s) 304
PfeI GAWTC 7 cut(s) 83, 424, 1039, 1376, 1586, 2188, 2857
PflFI GACNNNGTC 1 cut(s) 432
PfoI TCCNGGA 2 cut(s) 332, 744
PinAI ACCGGT 1 cut(s) 219
PleI GAGTC 7 cut(s) 248, 406, 423, 464, 798, 1091, 2241
PluTI GGCGCC 1 cut(s) 915
PpsI GAGTC 7 cut(s) 248, 406, 423, 464, 798, 1091, 2241
Ppu21I YACGTR 1 cut(s) 1446
PscI ACATGT 1 cut(s) 2688
PshAI GACNNNNGTC 1 cut(s) 789
Psp124BI GAGCTC 5 cut(s) 288, 582, 957, 1712, 2624
Psp6I CCWGG 7 cut(s) 176, 297, 332, 555, 1211, 1434, 3049
PspFI CCCAGC 2 cut(s) 1108, 2548
PspGI CCWGG 7 cut(s) 176, 297, 332, 555, 1211, 1434, 3049
PspPI GGNCC 5 cut(s) 99, 1130, 1532, 1750, 2699
PspXI VCTCGAGB 1 cut(s) 575
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
PstI CTGCAG 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 177
PsuI RGATCY 4 cut(s) 1076, 1700, 2302, 2581
PsyI GACNNNGTC 1 cut(s) 432
PteI GCGCGC 1 cut(s) 921
RsaI GTAC 5 cut(s) 1052, 1202, 1262, 1459, 2337
RsaNI GTAC 5 cut(s) 1051, 1201, 1261, 1458, 2336
RseI CAYNNNNRTG 3 cut(s) 1034, 1448, 2991
SacI GAGCTC 5 cut(s) 288, 582, 957, 1712, 2624
SalI GTCGAC 1 cut(s) 1439
SaqAI TTAA 4 cut(s) 413, 1851, 1995, 2961
Sau96I GGNCC 5 cut(s) 99, 1130, 1532, 1750, 2699
SchI GAGTC 7 cut(s) 248, 407, 423, 464, 799, 1092, 2241
SduI GDGCHC 9 cut(s) 164, 288, 582, 783, 957, 1074, 1712, 1921, 2624
SfaNI GCATC 3 cut(s) 978, 2060, 2866
SfcI CTRYAG 2 cut(s) 68, 845
SfoI GGCGCC 1 cut(s) 913
Sfr274I CTCGAG 2 cut(s) 575, 1094
SfuI TTCGAA 2 cut(s) 488, 2633
SinI GGWCC 2 cut(s) 1130, 1532
SlaI CTCGAG 2 cut(s) 575, 1094
SmaI CCCGGG 1 cut(s) 1410
SmiMI CAYNNNNRTG 3 cut(s) 1034, 1448, 2991
SmlI CTYRAG 7 cut(s) 116, 575, 1094, 1517, 1850, 2773, 2917
SmoI CTYRAG 7 cut(s) 116, 575, 1094, 1517, 1850, 2773, 2917
SseBI AGGCCT 1 cut(s) 1728
SspDI GGCGCC 1 cut(s) 911
SspI AATATT 1 cut(s) 2929
SspMI CTAG 8 cut(s) 731, 935, 2057, 2228, 2291, 2499, 2753, 2979
SstI GAGCTC 5 cut(s) 288, 582, 957, 1712, 2624
StuI AGGCCT 1 cut(s) 1728
StyI CCWWGG 1 cut(s) 583
TaaI ACNGT 1 cut(s) 762
TaiI ACGT 3 cut(s) 881, 1401, 1448
TaqII GACCGA 1 cut(s) 422
TatI WGTACW 2 cut(s) 1200, 1260
TauI GCSGC 4 cut(s) 234, 574, 603, 986
TfiI GAWTC 7 cut(s) 83, 424, 1039, 1376, 1586, 2188, 2857
Tru1I TTAA 4 cut(s) 413, 1851, 1995, 2961
Tru9I TTAA 4 cut(s) 413, 1851, 1995, 2961
TscAI CASTG 4 cut(s) 156, 1969, 2155, 2325
TseFI GTSAC 1 cut(s) 791
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 7 cut(s) 45, 967, 2298, 2412, 2496, 2529, 3028
TspGWI ACGGA 1 cut(s) 871
TspMI CCCGGG 1 cut(s) 1408
TspRI CASTG 4 cut(s) 156, 1969, 2155, 2325
Tth111I GACNNNGTC 1 cut(s) 432
Vha464I CTTAAG 1 cut(s) 1850
VneI GTGCAC 1 cut(s) 1917
VpaK11BI GGWCC 2 cut(s) 1130, 1532
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 9 cut(s) 685, 1547, 1755, 2259, 2555, 2724, 2947, 2966, 3000
XbaI TCTAGA 1 cut(s) 2290
XceI RCATGY 2 cut(s) 1597, 2692
XcmI CCANNNNNNNNNTGG 1 cut(s) 2651
XhoI CTCGAG 2 cut(s) 575, 1094
XmaI CCCGGG 1 cut(s) 1408
XmiI GTMKAC 1 cut(s) 1440
XspI CTAG 8 cut(s) 731, 935, 2057, 2228, 2291, 2499, 2753, 2979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.