Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010221

Physical Location & Seq

Genomic Coordinates Reverse (-)

3740549 .. 3741358

Gene Structure 810 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1048400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 810 bp

ATGGGTGTTCAAATACACAATTTGTCTCTATCCTCCCTGATTATTTTGATAATTTCAGCTGCAAGTCTGCAACTTTTAGCAGAATCCAAGACTTTCTGGGCCGACATTGAAGCTCTCAAGGAGTTCAAGAACTCACTCAACCTCGACTCAGTGAGTCCTGGTTCGTGCGTCAACTCCTGGGACTTCACCCTCAACCCCTGCGGCAACCTCTTCAATGATCGGTTCACCTGTGGCTTCCGCTGCGACCTGGTCAACAACTTCTCGTCCGCCAGCCAGCTCACCGAGCTCACCGAGCTCACCCTTGACCAGGTCAGTTACTTCGGCTCGCTCTCTTCCCTATCCTGGAACTTCCCGTATTTACAAACGCTTAACCTTTCTAACAACTTTTTCTCTGGATCGATCCCGGACTCGCTTTCTAACTTAACACGGCTGAGTCGACTCGGTCTCTCCGGCAACTCGTTCTTCGGCGCTATTCCCGACTCAATTGGATCCCTTTCGAACCTTGAGGAGCTTTTTCTCGACAACAACCGCCTTGACGGTGCAATCCCACAGAGCTTGCACGGCCTGGCGCAGTTGAAGCGGCTTGAGCTTCAGGGGAACCAGCTTGGCGGTGAGTTTCCCAAATTGGGTTCTCTCCAGAACCTCTACTACTTGGACGTCAGCAACAACGCCATATCTGGTGGCGTCCCAATAAATTTTCCGCCATCTTTGCTCCAAATCTCCATGCGAAACAACAGCTTAGAAGGAAGCATCCCGGAAGCAGAGGATGAGGAAGCAGCAAAGCTCAAGCTCTCCAATGGCGGTCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8

Protein Analysis

270

Amino Acids

29.22

Weight (kDa)

4.55

Isoelectric Point (pI)

34.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	111 - 221	3.1e-09	Leucine-rich repeat region
LRR_8	PF13855	160 - 202	1.7e-08	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66330
fragaria_vesca	FvH4_1g12241
malus_domestica	MD02G1136300.v1.1 MD08G1048400.v1.1 MD15G1250000.v1.1
prunus_persica	Prupe.2G102000_v2.0.a1 Prupe.7G164300_v2.0.a1
pyrus_communis	pycom15g22030
rosa_chinensis	RchiOBHm_Chr2g0100031
rosa_laevigata	RLG00000016931
rosa_multiflora	Rmu_co8338191.1_g000001 Rmu_sc0001616.1_g000058 Rmu_sc0006323.1_g000007
rosa_roxburghii	Rroxscaffold_2G00142620
rosa_rugosa	Rorug02G0084800
rosa_samantha	Rh2AG133900 Rh2BG137600 Rh2CG139000 Rh2DG139300 Rh4BG311000
rosa_wichuraiana	Rw2G010440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	660
AccI	GTMKAC	1 cut(s)	436
AciI	CCGC	8 cut(s)	201, 238, 267, 529, 580, 609, 701, 801
AclWI	GGATC	4 cut(s)	394, 403, 483, 496
AcsI	RAATTY	1 cut(s)	694
AcuI	CTGAAG	1 cut(s)	575
AcyI	GRCGYC	2 cut(s)	657, 684
AfiI	CCNNNNNNNGG	3 cut(s)	307, 342, 626
AgsI	TTSAA	5 cut(s)	11, 110, 127, 214, 577
AjnI	CCWGG	6 cut(s)	157, 176, 246, 306, 341, 564
Alw21I	GWGCWC	2 cut(s)	288, 297
Alw26I	GTCTC	2 cut(s)	30, 449
AlwI	GGATC	4 cut(s)	394, 403, 483, 496
AoxI	GGCC	2 cut(s)	99, 562
ApeKI	GCWGC	3 cut(s)	59, 240, 776
ApoI	RAATTY	1 cut(s)	694
AspLEI	GCGC	2 cut(s)	470, 571
AspS9I	GGNCC	1 cut(s)	99
AsuC2I	CCSGG	2 cut(s)	404, 755
AsuHPI	GGTGA	6 cut(s)	178, 217, 271, 280, 289, 623
AsuII	TTCGAA	1 cut(s)	497
BamHI	GGATCC	1 cut(s)	488
BanII	GRGCYC	2 cut(s)	288, 297
Bbv12I	GWGCWC	2 cut(s)	288, 297
BbvI	GCAGC	3 cut(s)	46, 227, 788
BccI	CCATC	1 cut(s)	712
BceAI	ACGGC	2 cut(s)	443, 577
BciT130I	CCWGG	6 cut(s)	159, 178, 248, 308, 343, 566
BcnI	CCSGG	2 cut(s)	404, 755
BcoDI	GTCTC	2 cut(s)	30, 449
BfoI	RGCGCY	1 cut(s)	471
BisI	GCNGC	5 cut(s)	60, 202, 241, 581, 777
BlsI	GCNGC	5 cut(s)	61, 203, 242, 582, 778
Bme1390I	CCNGG	8 cut(s)	159, 178, 248, 308, 343, 404, 566, 755
BmgT120I	GGNCC	1 cut(s)	99
BmiI	GGNNCC	2 cut(s)	490, 599
BmrFI	CCNGG	8 cut(s)	159, 178, 248, 308, 343, 404, 566, 755
BmsI	GCATC	1 cut(s)	759
BpmI	CTGGAG	1 cut(s)	620
Bpu14I	TTCGAA	1 cut(s)	497
BpuEI	CTTGAG	4 cut(s)	101, 524, 605, 770
BpuMI	CCSGG	2 cut(s)	404, 755
Bsa29I	ATCGAT	1 cut(s)	398
BsaHI	GRCGYC	2 cut(s)	657, 684
BsaI	GGTCTC	1 cut(s)	449
BsaJI	CCNNGG	1 cut(s)	177
Bsc4I	CCNNNNNNNGG	3 cut(s)	307, 342, 626
BseBI	CCWGG	6 cut(s)	159, 178, 248, 308, 343, 566
BseCI	ATCGAT	1 cut(s)	398
BseDI	CCNNGG	1 cut(s)	177
BseGI	GGATG	2 cut(s)	750, 772
BseLI	CCNNNNNNNGG	3 cut(s)	307, 342, 626
BseMII	CTCAG	2 cut(s)	162, 422
BseRI	GAGGAG	1 cut(s)	521
BseXI	GCAGC	3 cut(s)	46, 227, 788
BshFI	GGCC	2 cut(s)	101, 564
BshVI	ATCGAT	1 cut(s)	398
BsiHKAI	GWGCWC	2 cut(s)	288, 297
BsiSI	CCGG	3 cut(s)	404, 450, 755
BslFI	GGGAC	2 cut(s)	194, 671
BslI	CCNNNNNNNGG	3 cut(s)	307, 342, 626
BsmAI	GTCTC	2 cut(s)	30, 449
BsmFI	GGGAC	2 cut(s)	194, 671
BsnI	GGCC	2 cut(s)	101, 564
Bso31I	GGTCTC	1 cut(s)	449
Bsp119I	TTCGAA	1 cut(s)	497
Bsp1286I	GDGCHC	2 cut(s)	288, 297
Bsp143I	GATC	4 cut(s)	217, 395, 399, 488
BspACI	CCGC	8 cut(s)	201, 238, 267, 529, 580, 609, 701, 801
BspANI	GGCC	2 cut(s)	101, 564
BspCNI	CTCAG	2 cut(s)	161, 423
BspDI	ATCGAT	1 cut(s)	398
BspLI	GGNNCC	2 cut(s)	490, 599
BspPI	GGATC	4 cut(s)	394, 403, 483, 496
BspT104I	TTCGAA	1 cut(s)	497
BspTNI	GGTCTC	1 cut(s)	449
BssECI	CCNNGG	1 cut(s)	177
BssMI	GATC	4 cut(s)	217, 395, 399, 488
BssNI	GRCGYC	2 cut(s)	657, 684
Bst2UI	CCWGG	6 cut(s)	159, 178, 248, 308, 343, 566
Bst4CI	ACNGT	1 cut(s)	539
Bst6I	CTCTTC	2 cut(s)	215, 337
BstACI	GRCGYC	2 cut(s)	657, 684
BstBI	TTCGAA	1 cut(s)	497
BstC8I	GCNNGC	4 cut(s)	271, 275, 326, 557
BstDEI	CTNAG	3 cut(s)	148, 431, 739
BstENI	CCTNNNNNAGG	1 cut(s)	305
BstF5I	GGATG	2 cut(s)	750, 772
BstH2I	RGCGCY	1 cut(s)	471
BstHHI	GCGC	2 cut(s)	470, 571
BstKTI	GATC	4 cut(s)	220, 398, 402, 491
BstMAI	GTCTC	2 cut(s)	30, 449
BstMBI	GATC	4 cut(s)	217, 395, 399, 488
BstMWI	GCNNNNNNNGC	7 cut(s)	240, 283, 292, 561, 577, 586, 709
BstNI	CCWGG	6 cut(s)	159, 178, 248, 308, 343, 566
BstSCI	CCNGG	8 cut(s)	157, 176, 246, 306, 341, 402, 564, 753
BstV1I	GCAGC	3 cut(s)	46, 227, 788
BstX2I	RGATCY	1 cut(s)	488
BstYI	RGATCY	1 cut(s)	488
Bsu15I	ATCGAT	1 cut(s)	398
BsuRI	GGCC	2 cut(s)	101, 564
BsuTUI	ATCGAT	1 cut(s)	398
BtsCI	GGATG	2 cut(s)	750, 772
BtsIMutI	CAGTG	1 cut(s)	156
Cac8I	GCNNGC	4 cut(s)	271, 275, 326, 557
CfoI	GCGC	2 cut(s)	470, 571
Cfr13I	GGNCC	1 cut(s)	99
ClaI	ATCGAT	1 cut(s)	398
CseI	GACGC	2 cut(s)	157, 673
CsiI	ACCWGGT	2 cut(s)	246, 306
CspCI	CAANNNNNGTGG	2 cut(s)	537, 572
CviAII	CATG	1 cut(s)	724
DdeI	CTNAG	3 cut(s)	148, 431, 739
DpnI	GATC	4 cut(s)	219, 397, 401, 490
DpnII	GATC	4 cut(s)	217, 395, 399, 488
Eam1104I	CTCTTC	2 cut(s)	215, 337
EarI	CTCTTC	2 cut(s)	215, 337
EciI	GGCGGA	2 cut(s)	256, 690
Ecl136II	GAGCTC	2 cut(s)	286, 295
Eco24I	GRGCYC	2 cut(s)	288, 297
Eco31I	GGTCTC	1 cut(s)	449
Eco53kI	GAGCTC	2 cut(s)	286, 295
Eco57I	CTGAAG	1 cut(s)	575
EcoICRI	GAGCTC	2 cut(s)	286, 295
EcoNI	CCTNNNNNAGG	1 cut(s)	305
EcoRII	CCWGG	6 cut(s)	157, 176, 246, 306, 341, 564
EcoT38I	GRGCYC	2 cut(s)	288, 297
FaeI	CATG	1 cut(s)	727
FaiI	YATR	2 cut(s)	674, 725
FaqI	GGGAC	2 cut(s)	194, 671
FatI	CATG	1 cut(s)	723
FblI	GTMKAC	1 cut(s)	436
Fnu4HI	GCNGC	5 cut(s)	60, 202, 241, 581, 777
FokI	GGATG	2 cut(s)	737, 779
FriOI	GRGCYC	2 cut(s)	288, 297
Fsp4HI	GCNGC	5 cut(s)	60, 202, 241, 581, 777
GlaI	GCGC	2 cut(s)	469, 570
GluI	GCNGC	5 cut(s)	60, 202, 241, 581, 777
GsuI	CTGGAG	1 cut(s)	620
HaeII	RGCGCY	1 cut(s)	471
HaeIII	GGCC	2 cut(s)	101, 564
HapII	CCGG	3 cut(s)	404, 450, 755
HgaI	GACGC	2 cut(s)	157, 673
HhaI	GCGC	2 cut(s)	470, 571
Hin1I	GRCGYC	2 cut(s)	657, 684
Hin1II	CATG	1 cut(s)	727
Hin6I	GCGC	2 cut(s)	468, 569
HinP1I	GCGC	2 cut(s)	468, 569
HincII	GTYRAC	3 cut(s)	172, 253, 437
HindII	GTYRAC	3 cut(s)	172, 253, 437
HinfI	GANTC	7 cut(s)	83, 146, 154, 407, 433, 438, 479
HpaII	CCGG	3 cut(s)	404, 450, 755
HphI	GGTGA	6 cut(s)	178, 217, 271, 280, 289, 623
Hpy166II	GTNNAC	4 cut(s)	172, 225, 253, 437
Hpy188III	TCNNGA	5 cut(s)	127, 393, 476, 518, 637
Hpy8I	GTNNAC	4 cut(s)	172, 225, 253, 437
HpyAV	CCTTC	1 cut(s)	737
HpyCH4III	ACNGT	1 cut(s)	539
HpyCH4IV	ACGT	1 cut(s)	657
HpyCH4V	TGCA	4 cut(s)	62, 70, 542, 559
HpyF10VI	GCNNNNNNNGC	7 cut(s)	240, 283, 292, 561, 577, 586, 709
HpyF3I	CTNAG	3 cut(s)	148, 431, 739
HpySE526I	ACGT	1 cut(s)	657
Hsp92I	GRCGYC	2 cut(s)	657, 684
Hsp92II	CATG	1 cut(s)	727
HspAI	GCGC	2 cut(s)	468, 569
Kzo9I	GATC	4 cut(s)	217, 395, 399, 488
LmnI	GCTCC	2 cut(s)	508, 717
Lsp1109I	GCAGC	3 cut(s)	46, 227, 788
LweI	GCATC	1 cut(s)	759
MabI	ACCWGGT	2 cut(s)	246, 306
MaeII	ACGT	1 cut(s)	657
MaeIII	GTNAC	2 cut(s)	314, 803
MalI	GATC	4 cut(s)	219, 397, 401, 490
MboI	GATC	4 cut(s)	217, 395, 399, 488
MboII	GAAGA	3 cut(s)	202, 324, 454
MfeI	CAATTG	1 cut(s)	483
MflI	RGATCY	1 cut(s)	488
MhlI	GDGCHC	2 cut(s)	288, 297
MluCI	AATT	5 cut(s)	19, 51, 483, 623, 694
MlyI	GAGTC	6 cut(s)	140, 163, 401, 432, 442, 473
MnlI	CCTC	8 cut(s)	43, 152, 200, 218, 499, 653, 757, 763
MseI	TTAA	2 cut(s)	369, 422
MspA1I	CMGCKG	2 cut(s)	59, 240
MspI	CCGG	3 cut(s)	404, 450, 755
MspR9I	CCNGG	8 cut(s)	159, 178, 248, 308, 343, 404, 566, 755
MunI	CAATTG	1 cut(s)	483
MvaI	CCWGG	6 cut(s)	159, 178, 248, 308, 343, 566
MwoI	GCNNNNNNNGC	7 cut(s)	240, 283, 292, 561, 577, 586, 709
NciI	CCSGG	2 cut(s)	404, 755
NdeII	GATC	4 cut(s)	217, 395, 399, 488
NlaIII	CATG	1 cut(s)	727
NlaIV	GGNNCC	2 cut(s)	490, 599
NmuCI	GTSAC	1 cut(s)	803
NspV	TTCGAA	1 cut(s)	497
PcsI	WCGNNNNNNNCGW	1 cut(s)	433
PfeI	GAWTC	1 cut(s)	83
PflFI	GACNNNGTC	3 cut(s)	248, 308, 441
PfoI	TCCNGGA	3 cut(s)	341, 402, 753
PkrI	GCNGC	5 cut(s)	61, 203, 242, 582, 778
PleI	GAGTC	6 cut(s)	140, 162, 401, 432, 441, 473
PpsI	GAGTC	6 cut(s)	140, 162, 401, 432, 441, 473
Psp124BI	GAGCTC	2 cut(s)	288, 297
Psp6I	CCWGG	6 cut(s)	157, 176, 246, 306, 341, 564
PspGI	CCWGG	6 cut(s)	157, 176, 246, 306, 341, 564
PspN4I	GGNNCC	2 cut(s)	490, 599
PspPI	GGNCC	1 cut(s)	99
PsrI	GAACNNNNNNTAC	2 cut(s)	632, 664
PsuI	RGATCY	1 cut(s)	488
PsyI	GACNNNGTC	3 cut(s)	248, 308, 441
PvuII	CAGCTG	1 cut(s)	59
SacI	GAGCTC	2 cut(s)	288, 297
SalI	GTCGAC	1 cut(s)	435
SaqAI	TTAA	2 cut(s)	369, 422
SatI	GCNGC	5 cut(s)	60, 202, 241, 581, 777
Sau3AI	GATC	4 cut(s)	217, 395, 399, 488
Sau96I	GGNCC	1 cut(s)	99
SchI	GAGTC	6 cut(s)	140, 163, 401, 432, 442, 473
ScrFI	CCNGG	8 cut(s)	159, 178, 248, 308, 343, 404, 566, 755
SduI	GDGCHC	2 cut(s)	288, 297
SexAI	ACCWGGT	2 cut(s)	246, 306
SfaNI	GCATC	1 cut(s)	759
SfuI	TTCGAA	1 cut(s)	497
SmlI	CTYRAG	4 cut(s)	116, 503, 584, 785
SmoI	CTYRAG	4 cut(s)	116, 503, 584, 785
Sse9I	AATT	5 cut(s)	19, 51, 483, 623, 694
SsiI	CCGC	8 cut(s)	201, 238, 267, 529, 580, 609, 701, 801
SstI	GAGCTC	2 cut(s)	288, 297
StyD4I	CCNGG	8 cut(s)	157, 176, 246, 306, 341, 402, 564, 753
TaaI	ACNGT	1 cut(s)	539
TaiI	ACGT	1 cut(s)	660
TaqI	TCGA	5 cut(s)	144, 398, 436, 497, 519
TaqII	GACCGA	1 cut(s)	431
TasI	AATT	5 cut(s)	19, 51, 483, 623, 694
TauI	GCSGC	2 cut(s)	204, 583
TfiI	GAWTC	1 cut(s)	83
Tru1I	TTAA	2 cut(s)	369, 422
Tru9I	TTAA	2 cut(s)	369, 422
TscAI	CASTG	1 cut(s)	156
TseFI	GTSAC	1 cut(s)	803
TseI	GCWGC	3 cut(s)	59, 240, 776
Tsp45I	GTSAC	1 cut(s)	803
TspRI	CASTG	1 cut(s)	156
Tth111I	GACNNNGTC	3 cut(s)	248, 308, 441
XagI	CCTNNNNNAGG	1 cut(s)	305
XapI	RAATTY	1 cut(s)	694
XmiI	GTMKAC	1 cut(s)	436
ZraI	GACGTC	1 cut(s)	658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.