Rroxscaffold_2G00142620

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010221
Physical Location & Seq
Forward (+)
80725057 .. 80726310
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00142620.1

Sequence Viewer

Length: 1254 bp
ATGGGTGTTCTTGTTCTGCTATTTACAGTCGCAAGTCTTCTCCTTCTAGCAGAATCCAAGACTTTCTGGGAAGACACTCAAGTTCTGAAGCAGCTGAAGAACACACTAGACCCCAACTCAGTGGCTCAAGGCTCCTGCATTAGCTCATGGGACTTCAAACTCGACCCCTGCGACTCTCTCTTCAGCGATCGGTTCACTTGTGGCTTCCGGTGCGACCTAGTCGACGACTCCGGTGTCAGCCGAGTCACCGAAATCGGCCTAGACCAAGCTGGTTACTTCGGTTCTTCACTCTCCTCCCTCTCCTGGAACCTCCCTTACTTGGAAACTCTAGACCTCTCTGGTAACTTCTTCTCCGGTTCACTCCCCGAGTCATTCTCCAACCTCACTCGGCTCCGTCGACTCATCCTCTCCGGAAACTCGTTCTCCGGCTCTATTCCAGCTTCAATTGGATCCCTCACCAATCTTGAAGAGCTCTTCCTCGACAACAATTACCTCCAAGGCTCGATCCCACCGAGTTTGAACAACCTGGTCAGCCTGAACCGGCTGGAAATCCAAGCTAATAGTCTCAGCGGCGAGTTTCCGAAGTTGGATTCCCTCACAAGCCTTTACTACTTAGACGCCGGCAACAATGCAATTACGGGTCAAGTCCCGTCCACACTCCCACCCTCGTTGATTCAAATCTCCATACGAAACAACAGCCTAGTCGGAACGGTCCCGGAAACCATCAAACAGTTGGGTTCTCTACAAGTCCTCGATCTGAGTCACAACCAACTCGGTGACTCCGTCCCGGCACACCTCTTTACCCACCCATCTCTCCAGCAGCTCACTCTCTCCTTCAACAAGTTCTCCTCCCTCCAAACCCCGAGCTCAGCCGGAATCCAAAGCCAGCTTATCGCCATCGACCTCAGCAGCAACAACTTACAAGGTTTCTTACCATCCTTCATGGCCCTAATGCCAAAGCTCTCCGCTTTGTCCCTCGAAAACAACAAGTTCACTGGTATAATCCCAACCCAGTACGCTTTCAAAGTGGCCGTACCCGGAACCGGAGTCTCGCCGTTCGTCCGGCTTTTGCTTGGCGGAAACTACCTGTACGGACCCTTACCCGGTCCTTTACTTAGTATGAAACCCGGTTCGGCAAACGTGGGTCTTGGCAATAACTGCCTGTACAGATGTCCAAGGGTTTTCTTCTTCTGTCAAGGTGGTGACCAAAAATCGTCATCCGAGTGTAGAAGCTTCGTCCCTGTAATCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

417

Amino Acids

44.99

Weight (kDa)

5.14

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 103 - 140 9.5e-06 Leucine rich repeat
LRR_14 PF23598 103 - 327 1.8e-13 Leucine-rich repeat region
LRR_4 PF12799 129 - 167 9e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 201 - 258 4.4e-06 Leucine rich repeat
LRR_8 PF13855 223 - 282 2.8e-09 Leucine rich repeat
LRR_8 PF13855 270 - 331 6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccI GTMKAC 2 cut(s) 222, 397
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 3 cut(s) 570, 966, 1077
AclWI GGATC 3 cut(s) 444, 457, 499
AcoI YGGCCR 1 cut(s) 1029
AcuI CTGAAG 3 cut(s) 107, 116, 166
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 4 cut(s) 1016, 1035, 1091, 1166
AfiI CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
AgsI TTSAA 7 cut(s) 157, 444, 467, 520, 677, 838, 1024
AjnI CCWGG 2 cut(s) 302, 525
Alw21I GWGCWC 2 cut(s) 474, 869
Alw26I GTCTC 2 cut(s) 569, 1054
AlwI GGATC 3 cut(s) 444, 457, 499
Ama87I CYCGRG 2 cut(s) 365, 862
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 3 cut(s) 256, 945, 1029
ApeKI GCWGC 3 cut(s) 91, 820, 909
AspS9I GGNCC 4 cut(s) 712, 946, 1094, 1106
AsuC2I CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
AsuHPI GGTGA 4 cut(s) 238, 448, 788, 1214
AvaI CYCGRG 2 cut(s) 365, 862
AvaII GGWCC 3 cut(s) 712, 1094, 1106
BamHI GGATCC 1 cut(s) 449
BanII GRGCYC 2 cut(s) 474, 869
BbsI GAAGAC 2 cut(s) 29, 78
Bbv12I GWGCWC 2 cut(s) 474, 869
BbvCI CCTCAGC 1 cut(s) 905
BbvI GCAGC 3 cut(s) 103, 832, 921
BccI CCATC 4 cut(s) 731, 817, 905, 943
BceAI ACGGC 2 cut(s) 1016, 1039
BciT130I CCWGG 2 cut(s) 304, 527
BcnI CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
BcoDI GTCTC 2 cut(s) 569, 1054
BfaI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
BisI GCNGC 4 cut(s) 92, 571, 821, 910
BlpI GCTNAGC 1 cut(s) 868
BlsI GCNGC 4 cut(s) 93, 572, 822, 911
Bme1390I CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
Bme18I GGWCC 3 cut(s) 712, 1094, 1106
BmeT110I CYCGRG 2 cut(s) 365, 862
BmgT120I GGNCC 4 cut(s) 712, 946, 1094, 1106
BmiI GGNNCC 7 cut(s) 133, 308, 392, 451, 714, 1042, 1096
BmrFI CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
BmrI ACTGGG 1 cut(s) 1006
BmuI ACTGGG 1 cut(s) 1006
BpiI GAAGAC 2 cut(s) 29, 78
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 905
Bpu1102I GCTNAGC 1 cut(s) 868
BpuEI CTTGAG 2 cut(s) 63, 111
BpuMI CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 496, 1175
BsaWI WCCGGW 5 cut(s) 207, 230, 353, 410, 1043
BsaXI ACNNNNNCTCC 6 cut(s) 335, 365, 407, 437, 830, 860
Bsc4I CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
Bse118I RCCGGY 2 cut(s) 540, 620
Bse1I ACTGG 2 cut(s) 1000, 1012
Bse8I GATNNNNATC 1 cut(s) 677
BseAI TCCGGA 1 cut(s) 410
BseBI CCWGG 2 cut(s) 304, 527
BseDI CCNNGG 2 cut(s) 496, 1175
BseGI GGATG 3 cut(s) 402, 935, 1217
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
BseMII CTCAG 5 cut(s) 132, 580, 749, 882, 919
BseNI ACTGG 2 cut(s) 1000, 1012
BseRI GAGGAG 2 cut(s) 283, 838
BseXI GCAGC 3 cut(s) 103, 832, 921
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 258, 947, 1031
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 474, 869
BsiHKCI CYCGRG 2 cut(s) 365, 862
BslFI GGGAC 6 cut(s) 164, 632, 698, 770, 958, 1223
BslI CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
BsmAI GTCTC 2 cut(s) 569, 1054
BsmFI GGGAC 6 cut(s) 164, 632, 698, 770, 958, 1223
BsnI GGCC 3 cut(s) 258, 947, 1031
BsoBI CYCGRG 2 cut(s) 365, 862
Bsp1286I GDGCHC 2 cut(s) 474, 869
Bsp13I TCCGGA 1 cut(s) 410
Bsp1407I TGTACA 1 cut(s) 1164
Bsp143I GATC 4 cut(s) 187, 449, 504, 754
Bsp1720I GCTNAGC 1 cut(s) 868
BspACI CCGC 3 cut(s) 570, 966, 1077
BspANI GGCC 3 cut(s) 258, 947, 1031
BspCNI CTCAG 5 cut(s) 131, 579, 750, 881, 918
BspEI TCCGGA 1 cut(s) 410
BspLI GGNNCC 7 cut(s) 133, 308, 392, 451, 714, 1042, 1096
BspPI GGATC 3 cut(s) 444, 457, 499
BspQI GCTCTTC 2 cut(s) 462, 479
BsrFI RCCGGY 2 cut(s) 540, 620
BsrGI TGTACA 1 cut(s) 1164
BsrI ACTGG 2 cut(s) 1000, 1012
BssAI RCCGGY 2 cut(s) 540, 620
BssECI CCNNGG 2 cut(s) 496, 1175
BssMI GATC 4 cut(s) 187, 449, 504, 754
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 2 cut(s) 496, 1175
Bst2UI CCWGG 2 cut(s) 304, 527
Bst4CI ACNGT 3 cut(s) 28, 712, 732
Bst6I CTCTTC 3 cut(s) 185, 462, 479
BstACI GRCGYC 1 cut(s) 618
BstAPI GCANNNNNTGC 1 cut(s) 1158
BstAUI TGTACA 1 cut(s) 1164
BstC8I GCNNGC 2 cut(s) 622, 887
BstDEI CTNAG 7 cut(s) 118, 566, 613, 758, 868, 905, 1115
BstEII GGTNACC 1 cut(s) 1202
BstF5I GGATG 3 cut(s) 402, 935, 1217
BstKTI GATC 4 cut(s) 190, 452, 507, 757
BstMAI GTCTC 2 cut(s) 569, 1054
BstMBI GATC 4 cut(s) 187, 449, 504, 754
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 210, 1158
BstNI CCWGG 2 cut(s) 304, 527
BstPI GGTNACC 1 cut(s) 1202
BstSCI CCNGG 7 cut(s) 302, 525, 714, 786, 1036, 1102, 1126
BstV1I GCAGC 3 cut(s) 103, 832, 921
BstV2I GAAGAC 2 cut(s) 29, 78
BstX2I RGATCY 1 cut(s) 449
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 449
BsuRI GGCC 3 cut(s) 258, 947, 1031
BtsCI GGATG 3 cut(s) 402, 935, 1217
BtsIMutI CAGTG 2 cut(s) 126, 993
Cac8I GCNNGC 2 cut(s) 622, 887
Cfr10I RCCGGY 2 cut(s) 540, 620
Cfr13I GGNCC 4 cut(s) 712, 946, 1094, 1106
CseI GACGC 1 cut(s) 626
CsiI ACCWGGT 1 cut(s) 525
Csp6I GTAC 4 cut(s) 1015, 1034, 1090, 1165
CspCI CAANNNNNGTGG 4 cut(s) 498, 533, 651, 686
CviAII CATG 2 cut(s) 147, 943
CviQI GTAC 4 cut(s) 1015, 1034, 1090, 1165
DdeI CTNAG 7 cut(s) 118, 566, 613, 758, 868, 905, 1115
DpnI GATC 4 cut(s) 189, 451, 506, 756
DpnII GATC 4 cut(s) 187, 449, 504, 754
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 3 cut(s) 185, 462, 479
EarI CTCTTC 3 cut(s) 185, 462, 479
EciI GGCGGA 1 cut(s) 1092
Ecl136II GAGCTC 2 cut(s) 472, 867
Eco130I CCWWGG 2 cut(s) 496, 1175
Eco24I GRGCYC 2 cut(s) 474, 869
Eco47I GGWCC 3 cut(s) 712, 1094, 1106
Eco53kI GAGCTC 2 cut(s) 472, 867
Eco57I CTGAAG 3 cut(s) 107, 116, 166
Eco88I CYCGRG 2 cut(s) 365, 862
Eco91I GGTNACC 1 cut(s) 1202
EcoICRI GAGCTC 2 cut(s) 472, 867
EcoO65I GGTNACC 1 cut(s) 1202
EcoRII CCWGG 2 cut(s) 302, 525
EcoT14I CCWWGG 2 cut(s) 496, 1175
EcoT38I GRGCYC 2 cut(s) 474, 869
ErhI CCWWGG 2 cut(s) 496, 1175
FaeI CATG 2 cut(s) 150, 946
FaiI YATR 5 cut(s) 148, 686, 944, 1001, 1121
FaqI GGGAC 6 cut(s) 164, 632, 698, 770, 958, 1223
FatI CATG 2 cut(s) 146, 942
FblI GTMKAC 2 cut(s) 222, 397
Fnu4HI GCNGC 4 cut(s) 92, 571, 821, 910
FokI GGATG 3 cut(s) 389, 922, 1204
FriOI GRGCYC 2 cut(s) 474, 869
Fsp4HI GCNGC 4 cut(s) 92, 571, 821, 910
FspBI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
GluI GCNGC 4 cut(s) 92, 571, 821, 910
GsuI CTGGAG 1 cut(s) 800
HaeIII GGCC 3 cut(s) 258, 947, 1031
HgaI GACGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 2 cut(s) 150, 946
HincII GTYRAC 2 cut(s) 223, 398
HindII GTYRAC 2 cut(s) 223, 398
HindIII AAGCTT 1 cut(s) 1231
HphI GGTGA 4 cut(s) 238, 448, 788, 1214
Hpy166II GTNNAC 6 cut(s) 195, 223, 359, 398, 654, 993
Hpy188I TCNGA 5 cut(s) 87, 582, 707, 759, 1222
Hpy188III TCNNGA 3 cut(s) 329, 411, 464
Hpy8I GTNNAC 6 cut(s) 195, 223, 359, 398, 654, 993
Hpy99I CGWCG 2 cut(s) 227, 399
HpyAV CCTTC 3 cut(s) 53, 844, 949
HpyCH4III ACNGT 3 cut(s) 28, 712, 732
HpyCH4IV ACGT 1 cut(s) 1140
HpyCH4V TGCA 2 cut(s) 138, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 1158
HpyF3I CTNAG 7 cut(s) 118, 566, 613, 758, 868, 905, 1115
HpySE526I ACGT 1 cut(s) 1140
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 2 cut(s) 150, 946
Kpn2I TCCGGA 1 cut(s) 410
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Kzo9I GATC 4 cut(s) 187, 449, 504, 754
LguI GCTCTTC 2 cut(s) 462, 479
LmnI GCTCC 2 cut(s) 137, 396
Lsp1109I GCAGC 3 cut(s) 103, 832, 921
MabI ACCWGGT 1 cut(s) 525
MaeI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
MaeII ACGT 1 cut(s) 1140
MaeIII GTNAC 6 cut(s) 244, 272, 341, 761, 776, 1202
MalI GATC 4 cut(s) 189, 451, 506, 756
MboI GATC 4 cut(s) 187, 449, 504, 754
MfeI CAATTG 1 cut(s) 444
MflI RGATCY 1 cut(s) 449
MhlI GDGCHC 2 cut(s) 474, 869
MluCI AATT 3 cut(s) 444, 487, 633
MlyI GAGTC 8 cut(s) 167, 221, 252, 377, 393, 769, 773, 1056
MmeI TCCRAC 3 cut(s) 402, 567, 685
MroI TCCGGA 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 620
MslI CAYNNNNRTG 1 cut(s) 1222
MspA1I CMGCKG 2 cut(s) 94, 570
MspR9I CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
MunI CAATTG 1 cut(s) 444
MvaI CCWGG 2 cut(s) 304, 527
MwoI GCNNNNNNNGC 2 cut(s) 210, 1158
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
NdeII GATC 4 cut(s) 187, 449, 504, 754
NgoMIV GCCGGC 1 cut(s) 620
NlaIII CATG 2 cut(s) 150, 946
NlaIV GGNNCC 7 cut(s) 133, 308, 392, 451, 714, 1042, 1096
NmeAIII GCCGAG 2 cut(s) 266, 367
NmuCI GTSAC 4 cut(s) 244, 761, 776, 1202
PciSI GCTCTTC 2 cut(s) 462, 479
PcsI WCGNNNNNNNCGW 4 cut(s) 168, 394, 509, 780
PdiI GCCGGC 1 cut(s) 622
PfeI GAWTC 4 cut(s) 53, 590, 673, 876
PflFI GACNNNGTC 2 cut(s) 218, 782
PfoI TCCNGGA 2 cut(s) 302, 714
PkrI GCNGC 4 cut(s) 93, 572, 822, 911
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 8 cut(s) 167, 221, 251, 376, 393, 768, 773, 1055
PpsI GAGTC 8 cut(s) 167, 221, 251, 376, 393, 768, 773, 1055
Psp124BI GAGCTC 2 cut(s) 474, 869
Psp6I CCWGG 2 cut(s) 302, 525
PspEI GGTNACC 1 cut(s) 1202
PspGI CCWGG 2 cut(s) 302, 525
PspN4I GGNNCC 7 cut(s) 133, 308, 392, 451, 714, 1042, 1096
PspPI GGNCC 4 cut(s) 712, 946, 1094, 1106
PsrI GAACNNNNNNTAC 2 cut(s) 299, 331
PsuI RGATCY 1 cut(s) 449
PsyI GACNNNGTC 2 cut(s) 218, 782
PvuI CGATCG 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 94
RsaI GTAC 4 cut(s) 1016, 1035, 1091, 1166
RsaNI GTAC 4 cut(s) 1015, 1034, 1090, 1165
RseI CAYNNNNRTG 1 cut(s) 1222
SacI GAGCTC 2 cut(s) 474, 869
SalI GTCGAC 2 cut(s) 221, 396
SapI GCTCTTC 2 cut(s) 462, 479
SatI GCNGC 4 cut(s) 92, 571, 821, 910
Sau3AI GATC 4 cut(s) 187, 449, 504, 754
Sau96I GGNCC 4 cut(s) 712, 946, 1094, 1106
SchI GAGTC 8 cut(s) 167, 221, 252, 377, 393, 769, 773, 1056
ScrFI CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
SduI GDGCHC 2 cut(s) 474, 869
SexAI ACCWGGT 1 cut(s) 525
SinI GGWCC 3 cut(s) 712, 1094, 1106
SmiMI CAYNNNNRTG 1 cut(s) 1222
SmlI CTYRAG 2 cut(s) 78, 126
SmoI CTYRAG 2 cut(s) 78, 126
Sse9I AATT 3 cut(s) 444, 487, 633
SsiI CCGC 3 cut(s) 570, 966, 1077
SspMI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
SstI GAGCTC 2 cut(s) 474, 869
StyD4I CCNGG 7 cut(s) 302, 525, 714, 786, 1036, 1102, 1126
StyI CCWWGG 2 cut(s) 496, 1175
TaaI ACNGT 3 cut(s) 28, 712, 732
TaiI ACGT 1 cut(s) 1143
TaqI TCGA 8 cut(s) 162, 222, 397, 480, 503, 753, 900, 978
TasI AATT 3 cut(s) 444, 487, 633
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 1 cut(s) 573
TfiI GAWTC 4 cut(s) 53, 590, 673, 876
TscAI CASTG 2 cut(s) 126, 1000
TseFI GTSAC 4 cut(s) 244, 761, 776, 1202
TseI GCWGC 3 cut(s) 91, 820, 909
Tsp45I GTSAC 4 cut(s) 244, 761, 776, 1202
TspDTI ATGAA 2 cut(s) 931, 1136
TspGWI ACGGA 3 cut(s) 383, 772, 1107
TspRI CASTG 2 cut(s) 126, 1000
Tth111I GACNNNGTC 2 cut(s) 218, 782
VpaK11BI GGWCC 3 cut(s) 712, 1094, 1106
XbaI TCTAGA 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmiI GTMKAC 2 cut(s) 222, 397
XspI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.