RchiOBHm_Chr2g0100031

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010221
Physical Location & Seq
Reverse (-)
12054157 .. 12055672
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47471

Sequence Viewer

Length: 1254 bp
ATGGGTGTTCTTGTTCTGCTATTTACAGTCGCAAGTCTGCTCCTTCTAGCAGAATCCAAGACTTTCTGGGAAGACACTCAAGTTCTGAAGCAGCTGAAGAACAGACTAGACCCCAACTCAGTGGCTCAAGGCTCCTGCATTAGCTCATGGGACTTCAAACTCGACCCCTGCGACTCTCTCTTCAGCGATCGGTTCACTTGTGGCTTCCGGTGCGACCTAGTCGACGACTCCGGTGTCAGCCGAGTCACCGAAATCGGCCTAGACCAAGCTGGTTACTCCGGTTCTTCACTCTCCTCCCTTACCTGGAACCTCCCTTACTTGGAAACTCTAGACCTCTCTGGTAACTTCTTCTCCGGTTCACTCCCCGAGTCATTCTCCAACCTCACTCGGCTCCGTCGACTCATCCTCTCCGGAAACTCGTTCTCCGGCTCTATTCCAGCTTCAATTGGGTCCCTCACCAATCTTGAAGAGCTCTTCCTCGACAACAATTACCTCCGAGGCTCGATCCCGCCGAGTTTGAACAACCTGGTCAGCCTGAACCGGCTGGAAATCCAAGCTAATAGTCTCAGCGGCGAGTTTCCGAAGTTAGATTCCCTCACAAGCCTTTACTACTTAGACGCCGGCAACAATGCAATTACGGGTCAAGTCCCGTTCACATTCCCACCTTCGTTGATTCAAATCTCCATACGAAACAACAGCCTAGTCGGAACGGTCCCGGAAACCATCAAACAGTTGGGTTCTCTACAAGTCCTCGATCTGAGTCACAACCAACTCGGTGACTCCGTCCCGGCACACCTCTTTTCCCACCCATCTCTCCAGCAGCTCACTCTCTCCTTCAACAAGTTCTCCTCCCTCCAAACCCCGAGCTCAGCCGGATTCCAAAGCCAGCTCATCGCCGTCGACCTCAGCAACAACAACTTACAAGGTTTCTTACCATCCTTCATGGCCCTAATGCCAAAGCTCTCCGCTTTGTCCCTCGAAAACAACAAGTTCACTGGTATGATCCCAACCCAGTACGCCTTCAAAGTGGCCGTACCCGGAACCGGAGTCTCGCCGTTCGTCCGGCTTTTGCTCGGCGGAAACTACCTGTACGGACCCTTACCCGGTCCTTTACTTAGTATGAAACCCGGTTCGGCGAACGTGGGTCTTGGCAATAACTGCCTGTACAGATGTCCAAGGGTTTTCTTCTTTTGTCAAGGCGGTGACCAAAAATCGTCATCCGAGTGTAGAAGCTTCGTCCCTGTAATTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

417

Amino Acids

45.17

Weight (kDa)

5.47

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 95 - 327 4.3e-15 Leucine-rich repeat region
LRR_8 PF13855 103 - 140 7.3e-06 Leucine rich repeat
LRR_4 PF12799 129 - 169 6.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 201 - 258 6.5e-07 Leucine rich repeat
LRR_8 PF13855 223 - 282 1.3e-09 Leucine rich repeat
LRR_8 PF13855 270 - 331 2.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccI GTMKAC 3 cut(s) 222, 397, 900
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 5 cut(s) 509, 570, 966, 1077, 1200
AclWI GGATC 2 cut(s) 499, 997
AcoI YGGCCR 1 cut(s) 1029
AcuI CTGAAG 3 cut(s) 107, 116, 166
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 4 cut(s) 1016, 1035, 1091, 1166
AfiI CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
AgsI TTSAA 7 cut(s) 157, 444, 467, 520, 677, 838, 1024
AjnI CCWGG 2 cut(s) 302, 525
Alw21I GWGCWC 2 cut(s) 474, 869
Alw26I GTCTC 2 cut(s) 569, 1054
AlwI GGATC 2 cut(s) 499, 997
Ama87I CYCGRG 2 cut(s) 365, 862
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 3 cut(s) 256, 945, 1029
ApeKI GCWGC 2 cut(s) 91, 820
AspS9I GGNCC 5 cut(s) 450, 712, 946, 1094, 1106
AsuC2I CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
AsuHPI GGTGA 4 cut(s) 238, 448, 788, 1214
AvaI CYCGRG 2 cut(s) 365, 862
AvaII GGWCC 4 cut(s) 450, 712, 1094, 1106
BanII GRGCYC 2 cut(s) 474, 869
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 474, 869
BbvCI CCTCAGC 1 cut(s) 905
BbvI GCAGC 2 cut(s) 103, 832
BccI CCATC 3 cut(s) 731, 817, 943
BceAI ACGGC 3 cut(s) 881, 1016, 1039
BciT130I CCWGG 2 cut(s) 304, 527
BcnI CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
BcoDI GTCTC 2 cut(s) 569, 1054
BfaI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
BisI GCNGC 3 cut(s) 92, 571, 821
BlpI GCTNAGC 1 cut(s) 868
BlsI GCNGC 3 cut(s) 93, 572, 822
Bme1390I CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
Bme18I GGWCC 4 cut(s) 450, 712, 1094, 1106
BmeT110I CYCGRG 2 cut(s) 365, 862
BmgT120I GGNCC 5 cut(s) 450, 712, 946, 1094, 1106
BmiI GGNNCC 8 cut(s) 133, 308, 392, 451, 452, 714, 1042, 1096
BmrFI CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
BmrI ACTGGG 1 cut(s) 1006
BmuI ACTGGG 1 cut(s) 1006
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 905
Bpu1102I GCTNAGC 1 cut(s) 868
BpuEI CTTGAG 2 cut(s) 63, 111
BpuMI CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 496, 1175
BsaWI WCCGGW 6 cut(s) 207, 230, 278, 353, 410, 1043
BsaXI ACNNNNNCTCC 6 cut(s) 335, 365, 407, 437, 830, 860
Bsc4I CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
Bse118I RCCGGY 2 cut(s) 540, 620
Bse1I ACTGG 2 cut(s) 1000, 1012
Bse8I GATNNNNATC 1 cut(s) 677
BseAI TCCGGA 1 cut(s) 410
BseBI CCWGG 2 cut(s) 304, 527
BseDI CCNNGG 2 cut(s) 496, 1175
BseGI GGATG 3 cut(s) 402, 935, 1217
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
BseMII CTCAG 5 cut(s) 132, 580, 749, 882, 919
BseNI ACTGG 2 cut(s) 1000, 1012
BseRI GAGGAG 2 cut(s) 283, 838
BseXI GCAGC 2 cut(s) 103, 832
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 258, 947, 1031
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 474, 869
BsiHKCI CYCGRG 2 cut(s) 365, 862
BslFI GGGAC 7 cut(s) 164, 436, 632, 698, 770, 958, 1223
BslI CCNNNNNNNGG 4 cut(s) 303, 319, 1043, 1103
BsmAI GTCTC 2 cut(s) 569, 1054
BsmFI GGGAC 7 cut(s) 164, 436, 632, 698, 770, 958, 1223
BsnI GGCC 3 cut(s) 258, 947, 1031
BsoBI CYCGRG 2 cut(s) 365, 862
Bsp1286I GDGCHC 2 cut(s) 474, 869
Bsp13I TCCGGA 1 cut(s) 410
Bsp1407I TGTACA 1 cut(s) 1164
Bsp143I GATC 4 cut(s) 187, 504, 754, 1002
Bsp1720I GCTNAGC 1 cut(s) 868
BspACI CCGC 5 cut(s) 509, 570, 966, 1077, 1200
BspANI GGCC 3 cut(s) 258, 947, 1031
BspCNI CTCAG 5 cut(s) 131, 579, 750, 881, 918
BspEI TCCGGA 1 cut(s) 410
BspLI GGNNCC 8 cut(s) 133, 308, 392, 451, 452, 714, 1042, 1096
BspPI GGATC 2 cut(s) 499, 997
BspQI GCTCTTC 2 cut(s) 462, 479
BsrFI RCCGGY 2 cut(s) 540, 620
BsrGI TGTACA 1 cut(s) 1164
BsrI ACTGG 2 cut(s) 1000, 1012
BssAI RCCGGY 2 cut(s) 540, 620
BssECI CCNNGG 2 cut(s) 496, 1175
BssMI GATC 4 cut(s) 187, 504, 754, 1002
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 1175
Bst2UI CCWGG 2 cut(s) 304, 527
Bst4CI ACNGT 3 cut(s) 28, 712, 732
Bst6I CTCTTC 3 cut(s) 185, 462, 479
BstACI GRCGYC 1 cut(s) 618
BstAPI GCANNNNNTGC 1 cut(s) 1158
BstAUI TGTACA 1 cut(s) 1164
BstC8I GCNNGC 2 cut(s) 622, 887
BstDEI CTNAG 7 cut(s) 118, 566, 613, 758, 868, 905, 1115
BstEII GGTNACC 1 cut(s) 1202
BstF5I GGATG 3 cut(s) 402, 935, 1217
BstKTI GATC 4 cut(s) 190, 507, 757, 1005
BstMAI GTCTC 2 cut(s) 569, 1054
BstMBI GATC 4 cut(s) 187, 504, 754, 1002
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 210, 1158
BstNI CCWGG 2 cut(s) 304, 527
BstPI GGTNACC 1 cut(s) 1202
BstSCI CCNGG 7 cut(s) 302, 525, 714, 786, 1036, 1102, 1126
BstV1I GCAGC 2 cut(s) 103, 832
BstV2I GAAGAC 1 cut(s) 78
BstXI CCANNNNNNTGG 1 cut(s) 121
BsuRI GGCC 3 cut(s) 258, 947, 1031
BtgZI GCGATG 1 cut(s) 877
BtsCI GGATG 3 cut(s) 402, 935, 1217
BtsIMutI CAGTG 2 cut(s) 126, 993
Cac8I GCNNGC 2 cut(s) 622, 887
Cfr10I RCCGGY 2 cut(s) 540, 620
Cfr13I GGNCC 5 cut(s) 450, 712, 946, 1094, 1106
CseI GACGC 1 cut(s) 626
CsiI ACCWGGT 1 cut(s) 525
Csp6I GTAC 4 cut(s) 1015, 1034, 1090, 1165
CspCI CAANNNNNGTGG 2 cut(s) 651, 686
CviAII CATG 2 cut(s) 147, 943
CviQI GTAC 4 cut(s) 1015, 1034, 1090, 1165
DdeI CTNAG 7 cut(s) 118, 566, 613, 758, 868, 905, 1115
DpnI GATC 4 cut(s) 189, 506, 756, 1004
DpnII GATC 4 cut(s) 187, 504, 754, 1002
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 3 cut(s) 185, 462, 479
EarI CTCTTC 3 cut(s) 185, 462, 479
EciI GGCGGA 1 cut(s) 1092
Ecl136II GAGCTC 2 cut(s) 472, 867
Eco130I CCWWGG 1 cut(s) 1175
Eco24I GRGCYC 2 cut(s) 474, 869
Eco47I GGWCC 4 cut(s) 450, 712, 1094, 1106
Eco53kI GAGCTC 2 cut(s) 472, 867
Eco57I CTGAAG 3 cut(s) 107, 116, 166
Eco88I CYCGRG 2 cut(s) 365, 862
Eco91I GGTNACC 1 cut(s) 1202
EcoICRI GAGCTC 2 cut(s) 472, 867
EcoO109I RGGNCCY 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 1202
EcoRII CCWGG 2 cut(s) 302, 525
EcoT14I CCWWGG 1 cut(s) 1175
EcoT38I GRGCYC 2 cut(s) 474, 869
ErhI CCWWGG 1 cut(s) 1175
FaeI CATG 2 cut(s) 150, 946
FaiI YATR 5 cut(s) 148, 686, 944, 1001, 1121
FaqI GGGAC 7 cut(s) 164, 436, 632, 698, 770, 958, 1223
FatI CATG 2 cut(s) 146, 942
FauI CCCGC 1 cut(s) 516
FblI GTMKAC 3 cut(s) 222, 397, 900
Fnu4HI GCNGC 3 cut(s) 92, 571, 821
FokI GGATG 3 cut(s) 389, 922, 1204
FriOI GRGCYC 2 cut(s) 474, 869
Fsp4HI GCNGC 3 cut(s) 92, 571, 821
FspBI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
GluI GCNGC 3 cut(s) 92, 571, 821
GsuI CTGGAG 1 cut(s) 800
HaeIII GGCC 3 cut(s) 258, 947, 1031
HgaI GACGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 2 cut(s) 150, 946
HincII GTYRAC 3 cut(s) 223, 398, 901
HindII GTYRAC 3 cut(s) 223, 398, 901
HindIII AAGCTT 1 cut(s) 1231
HphI GGTGA 4 cut(s) 238, 448, 788, 1214
Hpy166II GTNNAC 7 cut(s) 195, 223, 359, 398, 654, 901, 993
Hpy188I TCNGA 6 cut(s) 87, 497, 582, 707, 759, 1222
Hpy188III TCNNGA 3 cut(s) 329, 411, 464
Hpy8I GTNNAC 7 cut(s) 195, 223, 359, 398, 654, 901, 993
Hpy99I CGWCG 3 cut(s) 227, 399, 902
HpyAV CCTTC 5 cut(s) 53, 675, 844, 949, 1030
HpyCH4III ACNGT 3 cut(s) 28, 712, 732
HpyCH4IV ACGT 1 cut(s) 1140
HpyCH4V TGCA 2 cut(s) 138, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 1158
HpyF3I CTNAG 7 cut(s) 118, 566, 613, 758, 868, 905, 1115
HpySE526I ACGT 1 cut(s) 1140
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 2 cut(s) 150, 946
KflI GGGWCCC 1 cut(s) 450
Kpn2I TCCGGA 1 cut(s) 410
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Kzo9I GATC 4 cut(s) 187, 504, 754, 1002
LguI GCTCTTC 2 cut(s) 462, 479
LmnI GCTCC 3 cut(s) 45, 137, 396
Lsp1109I GCAGC 2 cut(s) 103, 832
MabI ACCWGGT 1 cut(s) 525
MaeI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
MaeII ACGT 1 cut(s) 1140
MaeIII GTNAC 6 cut(s) 244, 272, 341, 761, 776, 1202
MalI GATC 4 cut(s) 189, 506, 756, 1004
MboI GATC 4 cut(s) 187, 504, 754, 1002
MboII GAAGA 8 cut(s) 83, 109, 172, 276, 340, 466, 479, 1177
MfeI CAATTG 1 cut(s) 444
MhlI GDGCHC 2 cut(s) 474, 869
MluCI AATT 4 cut(s) 444, 487, 633, 1245
MlyI GAGTC 8 cut(s) 167, 221, 252, 377, 393, 769, 773, 1056
MmeI TCCRAC 2 cut(s) 402, 685
MroI TCCGGA 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 620
MslI CAYNNNNRTG 2 cut(s) 998, 1222
MspA1I CMGCKG 2 cut(s) 94, 570
MspR9I CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
MunI CAATTG 1 cut(s) 444
MvaI CCWGG 2 cut(s) 304, 527
MwoI GCNNNNNNNGC 2 cut(s) 210, 1158
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 5 cut(s) 716, 788, 1038, 1104, 1128
NdeII GATC 4 cut(s) 187, 504, 754, 1002
NgoMIV GCCGGC 1 cut(s) 620
NlaIII CATG 2 cut(s) 150, 946
NlaIV GGNNCC 8 cut(s) 133, 308, 392, 451, 452, 714, 1042, 1096
NmeAIII GCCGAG 4 cut(s) 266, 367, 537, 1053
NmuCI GTSAC 4 cut(s) 244, 761, 776, 1202
PciSI GCTCTTC 2 cut(s) 462, 479
PcsI WCGNNNNNNNCGW 4 cut(s) 168, 394, 509, 780
PdiI GCCGGC 1 cut(s) 622
PfeI GAWTC 4 cut(s) 53, 590, 673, 876
PflFI GACNNNGTC 2 cut(s) 218, 782
PfoI TCCNGGA 1 cut(s) 714
PkrI GCNGC 3 cut(s) 93, 572, 822
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 8 cut(s) 167, 221, 251, 376, 393, 768, 773, 1055
PpsI GAGTC 8 cut(s) 167, 221, 251, 376, 393, 768, 773, 1055
PpuMI RGGWCCY 1 cut(s) 450
Psp124BI GAGCTC 2 cut(s) 474, 869
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 302, 525
PspEI GGTNACC 1 cut(s) 1202
PspGI CCWGG 2 cut(s) 302, 525
PspN4I GGNNCC 8 cut(s) 133, 308, 392, 451, 452, 714, 1042, 1096
PspPI GGNCC 5 cut(s) 450, 712, 946, 1094, 1106
PspPPI RGGWCCY 1 cut(s) 450
PsrI GAACNNNNNNTAC 2 cut(s) 299, 331
PsyI GACNNNGTC 2 cut(s) 218, 782
PvuI CGATCG 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 94
RsaI GTAC 4 cut(s) 1016, 1035, 1091, 1166
RsaNI GTAC 4 cut(s) 1015, 1034, 1090, 1165
RseI CAYNNNNRTG 2 cut(s) 998, 1222
SacI GAGCTC 2 cut(s) 474, 869
SalI GTCGAC 3 cut(s) 221, 396, 899
SapI GCTCTTC 2 cut(s) 462, 479
SatI GCNGC 3 cut(s) 92, 571, 821
Sau3AI GATC 4 cut(s) 187, 504, 754, 1002
Sau96I GGNCC 5 cut(s) 450, 712, 946, 1094, 1106
SchI GAGTC 8 cut(s) 167, 221, 252, 377, 393, 769, 773, 1056
ScrFI CCNGG 7 cut(s) 304, 527, 716, 788, 1038, 1104, 1128
SduI GDGCHC 2 cut(s) 474, 869
SexAI ACCWGGT 1 cut(s) 525
SinI GGWCC 4 cut(s) 450, 712, 1094, 1106
SmiMI CAYNNNNRTG 2 cut(s) 998, 1222
SmlI CTYRAG 2 cut(s) 78, 126
SmoI CTYRAG 2 cut(s) 78, 126
Sse9I AATT 4 cut(s) 444, 487, 633, 1245
SsiI CCGC 5 cut(s) 509, 570, 966, 1077, 1200
SspMI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
SstI GAGCTC 2 cut(s) 474, 869
StyD4I CCNGG 7 cut(s) 302, 525, 714, 786, 1036, 1102, 1126
StyI CCWWGG 1 cut(s) 1175
TaaI ACNGT 3 cut(s) 28, 712, 732
TaiI ACGT 1 cut(s) 1143
TaqI TCGA 8 cut(s) 162, 222, 397, 480, 503, 753, 900, 978
TasI AATT 4 cut(s) 444, 487, 633, 1245
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 1 cut(s) 573
TfiI GAWTC 4 cut(s) 53, 590, 673, 876
TscAI CASTG 2 cut(s) 126, 1000
TseFI GTSAC 4 cut(s) 244, 761, 776, 1202
TseI GCWGC 2 cut(s) 91, 820
Tsp45I GTSAC 4 cut(s) 244, 761, 776, 1202
TspDTI ATGAA 2 cut(s) 931, 1136
TspGWI ACGGA 3 cut(s) 383, 772, 1107
TspRI CASTG 2 cut(s) 126, 1000
Tth111I GACNNNGTC 2 cut(s) 218, 782
VpaK11BI GGWCC 4 cut(s) 450, 712, 1094, 1106
XbaI TCTAGA 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmiI GTMKAC 3 cut(s) 222, 397, 900
XspI CTAG 6 cut(s) 47, 107, 218, 260, 329, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.