Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009050

Physical Location & Seq

Genomic Coordinates Reverse (-)

26503115 .. 26506809

Gene Structure 3695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G66350.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 996 bp

ATGGCAGGATTTTTCTCGTTAGGACACGGCGGAGGAGGAAACACTCCAGACAACCACAGAACAAACACTAATAATCCTTCTTCATCGGGAACAGAATCTTGGCTTTGGTGCAGAAACCCTAACTCTAACGCTGACGGTGGAGAAGCTGGTCCTTCTTACAAAGGAACCCTTGAGCTATGGCAACACCCAAACAATCAAGAAATCATTTTCCAGCAGCAGCAGCAACAGCAACAAAGGCTGGATCTTTACACTTCCGCTGCGGGTTTAGGTGTTGGACCGAGCAACCGGAGCTTAATTGAAACTTCCGGCGGTGCGTTGATGATGATGAGAAGCGGTAGCGGTAGCGGCGGACCAAGCTGCCAGGATTGTGGGAATCAATCTAAGAAAGACTGCTCTCACATGAGATGTAGGACTTGCTGCAAGAGCCGTGGCCTTGACTGTCCCACTCACGTGAAGAGCACGTGGGTTCCTGCCGCTAAACGCCGAGAACGCCAGCAGCAGCTTTCTACCGGTCAGCAACCGCAGCAACTGGGAGGGAGCGTCCCTAAACGACAGAGAGAGCGTATCCCGGCGAGATCGACTTCCATGGCCTACACTCGTATACCTTCTAACAACACTTCAGGGTTGGAGGTTGGGAATTTTCCGCCGGAAGTTAGCTCGTCGGCAGTTTTTCGGTGCGTGCGTGTGAGTTCCGTAGATGATGAAGAAGAAGAGTATGCATATAAAACAGCTGTGAGTATAGGCGGTCACGTCTTCAAAGGTGTTCTCTACGATCAAGGCCCGGCCGAGAGAAGCTCCTCGGGCGGTGGATCTCAGCCGTTGAATCTCATAACCGCAGGCCCATCGGCCTCATCATCAAGCCCAAACGTGAGCTGCAACAATGGAGTCGTTGGCTCCACTTCAGATCATTATATCGATCCTGCCTCACTTAATTATCCTACTCCCATTAACACTTTCATGACTGGTACGCACTTCTTCTCCAACTCAAGATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF702

Protein Analysis

331

Amino Acids

35.38

Weight (kDa)

8.52

Isoelectric Point (pI)

56.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF702	PF05142	117 - 262	1.6e-63	Domain of unknown function (DUF702)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51060 AT5G66350 AT5G66350 AT5G66350
fragaria_vesca	FvH4_1g12410
malus_domestica	MD02G1137000.v1.1 MD15G1250700.v1.1
prunus_persica	Prupe.7G163600_v2.0.a1 Prupe.7G163600_v2.0.a1 Prupe.7G163600_v2.0.a1
pyrus_communis	pycom02g10840 pycom15g22110
rosa_chinensis	RchiOBHm_Chr2g0100271
rosa_laevigata	RLG00000016951
rosa_multiflora	Rmu_sc0002547.1_g000016
rosa_roxburghii	Rroxscaffold_2G00142430
rosa_rugosa	Rorug02G0087000
rosa_samantha	Rh2AG136000 Rh2BG139600 Rh2CG141300 Rh2DG141500
rosa_wichuraiana	Rw2G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	601
AclWI	GGATC	3 cut(s)	249, 817, 911
AcoI	YGGCCR	1 cut(s)	783
AcsI	RAATTY	1 cut(s)	637
AcuI	CTGAAG	2 cut(s)	603, 885
AcvI	CACGTG	2 cut(s)	451, 462
AfaI	GTAC	1 cut(s)	967
AgeI	ACCGGT	1 cut(s)	509
AgsI	TTSAA	3 cut(s)	299, 757, 823
AjiI	CACGTC	1 cut(s)	751
AjnI	CCWGG	1 cut(s)	360
AjuI	GAANNNNNNNTTGG	2 cut(s)	82, 114
AleI	CACNNNNGTG	1 cut(s)	449
AluBI	AGCT	9 cut(s)	146, 175, 291, 357, 502, 657, 731, 795, 873
AluI	AGCT	9 cut(s)	146, 175, 291, 357, 502, 657, 731, 795, 873
Alw21I	GWGCWC	1 cut(s)	461
AlwI	GGATC	3 cut(s)	249, 817, 911
AlwNI	CAGNNNCTG	1 cut(s)	529
Ama87I	CYCGRG	1 cut(s)	799
AoxI	GGCC	6 cut(s)	430, 588, 778, 783, 838, 846
ApoI	RAATTY	1 cut(s)	637
AsiGI	ACCGGT	1 cut(s)	509
AspS9I	GGNCC	5 cut(s)	149, 275, 350, 779, 839
AsuC2I	CCSGG	2 cut(s)	569, 782
AvaI	CYCGRG	1 cut(s)	799
AvaII	GGWCC	3 cut(s)	149, 275, 350
BbrPI	CACGTG	2 cut(s)	451, 462
BbsI	GAAGAC	1 cut(s)	745
Bbv12I	GWGCWC	1 cut(s)	461
BccI	CCATC	1 cut(s)	850
BceAI	ACGGC	3 cut(s)	43, 411, 802
BcgI	CGANNNNNNTGC	2 cut(s)	825, 859
BciT130I	CCWGG	1 cut(s)	362
BciVI	GTATCC	1 cut(s)	575
BcnI	CCSGG	2 cut(s)	569, 782
BfuI	GTATCC	1 cut(s)	575
BglII	AGATCT	1 cut(s)	989
Bme1390I	CCNGG	3 cut(s)	362, 569, 782
Bme18I	GGWCC	3 cut(s)	149, 275, 350
BmeT110I	CYCGRG	1 cut(s)	799
BmgBI	CACGTC	1 cut(s)	751
BmgT120I	GGNCC	5 cut(s)	149, 275, 350, 779, 839
BmiI	GGNNCC	3 cut(s)	166, 468, 895
BmrFI	CCNGG	3 cut(s)	362, 569, 782
BmrI	ACTGGG	1 cut(s)	539
BmuI	ACTGGG	1 cut(s)	539
BpiI	GAAGAC	1 cut(s)	745
BplI	GAGNNNNNCTC	2 cut(s)	779, 811
BpmI	CTGGAG	1 cut(s)	30
BpuEI	CTTGAG	2 cut(s)	191, 970
BpuMI	CCSGG	2 cut(s)	569, 782
Bsa29I	ATCGAT	1 cut(s)	915
BsaAI	YACGTR	2 cut(s)	451, 462
BsaJI	CCNNGG	3 cut(s)	427, 585, 798
BsaWI	WCCGGW	2 cut(s)	285, 509
BsaXI	ACNNNNNCTCC	4 cut(s)	525, 555, 962, 992
Bse118I	RCCGGY	1 cut(s)	509
Bse1I	ACTGG	2 cut(s)	534, 967
BseBI	CCWGG	1 cut(s)	362
BseCI	ATCGAT	1 cut(s)	915
BseDI	CCNNGG	3 cut(s)	427, 585, 798
BseMII	CTCAG	1 cut(s)	827
BseNI	ACTGG	2 cut(s)	534, 967
BseRI	GAGGAG	2 cut(s)	48, 787
BseX3I	CGGCCG	1 cut(s)	783
BsgI	GTGCAG	1 cut(s)	130
Bsh1285I	CGRYCG	1 cut(s)	786
BshFI	GGCC	6 cut(s)	432, 590, 780, 785, 840, 848
BshTI	ACCGGT	1 cut(s)	509
BshVI	ATCGAT	1 cut(s)	915
BsiEI	CGRYCG	1 cut(s)	786
BsiHKAI	GWGCWC	1 cut(s)	461
BsiHKCI	CYCGRG	1 cut(s)	799
BsiSI	CCGG	6 cut(s)	286, 306, 510, 569, 647, 782
BslFI	GGGAC	2 cut(s)	426, 527
BsmFI	GGGAC	2 cut(s)	426, 527
BsnI	GGCC	6 cut(s)	432, 590, 780, 785, 840, 848
BsoBI	CYCGRG	1 cut(s)	799
Bsp1286I	GDGCHC	1 cut(s)	461
Bsp143I	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
Bsp19I	CCATGG	1 cut(s)	585
BspANI	GGCC	6 cut(s)	432, 590, 780, 785, 840, 848
BspCNI	CTCAG	1 cut(s)	826
BspDI	ATCGAT	1 cut(s)	915
BspHI	TCATGA	1 cut(s)	957
BspLI	GGNNCC	3 cut(s)	166, 468, 895
BspPI	GGATC	3 cut(s)	249, 817, 911
BspQI	GCTCTTC	1 cut(s)	449
BsrFI	RCCGGY	1 cut(s)	509
BsrI	ACTGG	2 cut(s)	534, 967
BssAI	RCCGGY	1 cut(s)	509
BssECI	CCNNGG	3 cut(s)	427, 585, 798
BssMI	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
BssNAI	GTATAC	1 cut(s)	602
BssT1I	CCWWGG	1 cut(s)	585
Bst1107I	GTATAC	1 cut(s)	602
Bst2UI	CCWGG	1 cut(s)	362
Bst4CI	ACNGT	2 cut(s)	137, 440
Bst6I	CTCTTC	2 cut(s)	449, 705
BstBAI	YACGTR	2 cut(s)	451, 462
BstC8I	GCNNGC	3 cut(s)	494, 680, 838
BstDEI	CTNAG	2 cut(s)	381, 813
BstDSI	CCRYGG	2 cut(s)	427, 585
BstKTI	GATC	7 cut(s)	244, 578, 775, 812, 907, 919, 992
BstMBI	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
BstMCI	CGRYCG	1 cut(s)	786
BstNI	CCWGG	1 cut(s)	362
BstSCI	CCNGG	3 cut(s)	360, 567, 780
BstV2I	GAAGAC	1 cut(s)	745
BstX2I	RGATCY	3 cut(s)	241, 809, 989
BstXI	CCANNNNNNTGG	1 cut(s)	368
BstYI	RGATCY	3 cut(s)	241, 809, 989
BstZ17I	GTATAC	1 cut(s)	602
BstZI	CGGCCG	1 cut(s)	783
Bsu15I	ATCGAT	1 cut(s)	915
BsuI	GTATCC	1 cut(s)	575
BsuRI	GGCC	6 cut(s)	432, 590, 780, 785, 840, 848
BsuTUI	ATCGAT	1 cut(s)	915
BtgI	CCRYGG	2 cut(s)	427, 585
BtrI	CACGTC	1 cut(s)	751
Cac8I	GCNNGC	3 cut(s)	494, 680, 838
CaiI	CAGNNNCTG	1 cut(s)	529
CciI	TCATGA	1 cut(s)	957
Cfr10I	RCCGGY	1 cut(s)	509
Cfr13I	GGNCC	5 cut(s)	149, 275, 350, 779, 839
ClaI	ATCGAT	1 cut(s)	915
CseI	GACGC	1 cut(s)	529
Csp6I	GTAC	1 cut(s)	966
CspAI	ACCGGT	1 cut(s)	509
CspCI	CAANNNNNGTGG	2 cut(s)	409, 444
CviAII	CATG	3 cut(s)	400, 586, 958
CviQI	GTAC	1 cut(s)	966
DdeI	CTNAG	2 cut(s)	381, 813
DpnI	GATC	7 cut(s)	243, 577, 774, 811, 906, 918, 991
DpnII	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
EaeI	YGGCCR	1 cut(s)	783
EagI	CGGCCG	1 cut(s)	783
Eam1104I	CTCTTC	2 cut(s)	449, 705
EarI	CTCTTC	2 cut(s)	449, 705
EciI	GGCGGA	3 cut(s)	45, 363, 633
EclXI	CGGCCG	1 cut(s)	783
Eco130I	CCWWGG	1 cut(s)	585
Eco47I	GGWCC	3 cut(s)	149, 275, 350
Eco52I	CGGCCG	1 cut(s)	783
Eco57I	CTGAAG	2 cut(s)	603, 885
Eco72I	CACGTG	2 cut(s)	451, 462
Eco88I	CYCGRG	1 cut(s)	799
EcoRII	CCWGG	1 cut(s)	360
EcoT14I	CCWWGG	1 cut(s)	585
EcoT22I	ATGCAT	1 cut(s)	721
ErhI	CCWWGG	1 cut(s)	585
FaeI	CATG	3 cut(s)	403, 589, 961
FalI	AAGNNNNNCTT	2 cut(s)	153, 185
FaqI	GGGAC	2 cut(s)	426, 527
FatI	CATG	3 cut(s)	399, 585, 957
FauI	CCCGC	1 cut(s)	253
FblI	GTMKAC	1 cut(s)	601
GsuI	CTGGAG	1 cut(s)	30
HaeIII	GGCC	6 cut(s)	432, 590, 780, 785, 840, 848
HapII	CCGG	6 cut(s)	286, 306, 510, 569, 647, 782
HgaI	GACGC	1 cut(s)	529
Hin1II	CATG	3 cut(s)	403, 589, 961
HinfI	GANTC	4 cut(s)	95, 373, 823, 885
HpaII	CCGG	6 cut(s)	286, 306, 510, 569, 647, 782
Hpy166II	GTNNAC	1 cut(s)	602
Hpy188I	TCNGA	1 cut(s)	904
Hpy188III	TCNNGA	6 cut(s)	47, 87, 197, 958, 987, 993
Hpy8I	GTNNAC	1 cut(s)	602
Hpy99I	CGWCG	1 cut(s)	664
HpyAV	CCTTC	3 cut(s)	87, 162, 615
HpyCH4III	ACNGT	2 cut(s)	137, 440
HpyCH4IV	ACGT	4 cut(s)	450, 461, 750, 867
HpyCH4V	TGCA	4 cut(s)	111, 420, 719, 876
HpyF3I	CTNAG	2 cut(s)	381, 813
HpySE526I	ACGT	4 cut(s)	450, 461, 750, 867
Hsp92II	CATG	3 cut(s)	403, 589, 961
Kzo9I	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
LguI	GCTCTTC	1 cut(s)	449
LmnI	GCTCC	4 cut(s)	288, 537, 800, 899
MaeII	ACGT	4 cut(s)	450, 461, 750, 867
MaeIII	GTNAC	1 cut(s)	746
MalI	GATC	7 cut(s)	243, 577, 774, 811, 906, 918, 991
MboI	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
MboII	GAAGA	7 cut(s)	72, 466, 716, 719, 722, 745, 967
MflI	RGATCY	3 cut(s)	241, 809, 989
MhlI	GDGCHC	1 cut(s)	461
MluCI	AATT	3 cut(s)	294, 637, 931
MlyI	GAGTC	1 cut(s)	894
MmeI	TCCRAC	2 cut(s)	253, 606
MnlI	CCTC	7 cut(s)	26, 29, 527, 622, 808, 859, 934
Mph1103I	ATGCAT	1 cut(s)	721
MseI	TTAA	3 cut(s)	293, 930, 948
MslI	CAYNNNNRTG	2 cut(s)	449, 956
MspA1I	CMGCKG	2 cut(s)	257, 731
MspI	CCGG	6 cut(s)	286, 306, 510, 569, 647, 782
MspR9I	CCNGG	3 cut(s)	362, 569, 782
MvaI	CCWGG	1 cut(s)	362
NciI	CCSGG	2 cut(s)	569, 782
NcoI	CCATGG	1 cut(s)	585
NdeII	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
NlaIII	CATG	3 cut(s)	403, 589, 961
NlaIV	GGNNCC	3 cut(s)	166, 468, 895
NmeAIII	GCCGAG	2 cut(s)	509, 811
NmuCI	GTSAC	1 cut(s)	746
NsiI	ATGCAT	1 cut(s)	721
OliI	CACNNNNGTG	1 cut(s)	449
PagI	TCATGA	1 cut(s)	957
PciSI	GCTCTTC	1 cut(s)	449
PcsI	WCGNNNNNNNCGW	1 cut(s)	679
PfeI	GAWTC	3 cut(s)	95, 373, 823
PinAI	ACCGGT	1 cut(s)	509
PleI	GAGTC	1 cut(s)	893
PmaCI	CACGTG	2 cut(s)	451, 462
PmlI	CACGTG	2 cut(s)	451, 462
PpsI	GAGTC	1 cut(s)	893
Ppu21I	YACGTR	2 cut(s)	451, 462
Psp6I	CCWGG	1 cut(s)	360
PspCI	CACGTG	2 cut(s)	451, 462
PspGI	CCWGG	1 cut(s)	360
PspN4I	GGNNCC	3 cut(s)	166, 468, 895
PspPI	GGNCC	5 cut(s)	149, 275, 350, 779, 839
PstNI	CAGNNNCTG	1 cut(s)	529
PsuI	RGATCY	3 cut(s)	241, 809, 989
PvuII	CAGCTG	1 cut(s)	731
RsaI	GTAC	1 cut(s)	967
RsaNI	GTAC	1 cut(s)	966
RseI	CAYNNNNRTG	2 cut(s)	449, 956
SapI	GCTCTTC	1 cut(s)	449
SaqAI	TTAA	3 cut(s)	293, 930, 948
Sau3AI	GATC	7 cut(s)	241, 575, 772, 809, 904, 916, 989
Sau96I	GGNCC	5 cut(s)	149, 275, 350, 779, 839
SchI	GAGTC	1 cut(s)	894
ScrFI	CCNGG	3 cut(s)	362, 569, 782
SduI	GDGCHC	1 cut(s)	461
SinI	GGWCC	3 cut(s)	149, 275, 350
SmiMI	CAYNNNNRTG	2 cut(s)	449, 956
SmlI	CTYRAG	2 cut(s)	170, 985
SmoI	CTYRAG	2 cut(s)	170, 985
Sse9I	AATT	3 cut(s)	294, 637, 931
StyD4I	CCNGG	3 cut(s)	360, 567, 780
StyI	CCWWGG	1 cut(s)	585
TaaI	ACNGT	2 cut(s)	137, 440
TaiI	ACGT	4 cut(s)	453, 464, 753, 870
TaqI	TCGA	2 cut(s)	578, 915
TaqII	GACCGA	1 cut(s)	292
TasI	AATT	3 cut(s)	294, 637, 931
TauI	GCSGC	2 cut(s)	348, 476
TfiI	GAWTC	3 cut(s)	95, 373, 823
Tru1I	TTAA	3 cut(s)	293, 930, 948
Tru9I	TTAA	3 cut(s)	293, 930, 948
TseFI	GTSAC	1 cut(s)	746
Tsp45I	GTSAC	1 cut(s)	746
TspDTI	ATGAA	3 cut(s)	72, 717, 946
TspGWI	ACGGA	1 cut(s)	682
VpaK11BI	GGWCC	3 cut(s)	149, 275, 350
XapI	RAATTY	1 cut(s)	637
XmiI	GTMKAC	1 cut(s)	601
Zsp2I	ATGCAT	1 cut(s)	721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.