ATCG00270

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors. D2 is needed for assembly of a stable PSII complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0004028
Physical Location & Seq
Forward (+)
32711 .. 33772
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
ATCG00270.1

Sequence Viewer

Length: 1062 bp
ATGACTATAGCCCTTGGTAAATTTACCAAAGACGAAAAAGATTTATTTGATATTATGGATGACTGGTTACGGAGGGACCGCTTCGTTTTTGTAGGTTGGTCTGGTCTATTGCTCTTTCCTTGTGCCTATTTCGCTTTAGGGGGTTGGTTCACAGGTACAACCTTTGTAACTTCATGGTATACTCATGGATTGGCCAGTTCCTATTTAGAAGGTTGCAATTTTTTAACCGCTGCAGTTTCTACTCCTGCTAATAGTTTAGCGCATTCTTTGTTGTTACTGTGGGGTCCTGAAGCACAAGGAGATTTTACTCGTTGGTGTCAATTAGGTGGTCTATGGGCTTTTGTTGCTCTCCACGGCGCTTTCGCATTAATAGGTTTTATGTTACGTCAATTTGAACTTGCTCGATCTGTTCAATTGCGACCTTATAATGCAATCGCATTCTCTGGTCCCATTGCTGTTTTTGTTTCTGTCTTCCTAATTTATCCACTAGGTCAATCTGGTTGGTTCTTTGCGCCTAGTTTTGGTGTAGCGGCTATATTTCGATTCATCCTCTTTTTCCAAGGGTTTCATAATTGGACATTGAACCCATTTCATATGATGGGAGTCGCCGGTGTACTGGGCGCGGCTCTGCTATGCGCTATTCATGGTGCTACTGTAGAAAATACTTTATTTGAAGATGGTGATGGTGCAAATACATTCCGTGCTTTTAACCCAACTCAAGCCGAAGAAACTTATTCAATGGTCACCGCTAACCGCTTTTGGTCACAAATCTTTGGGGTTGCTTTTTCCAATAAACGTTGGTTACATTTCTTTATGTTATTTGTACCGGTAACTGGTTTATGGATGAGTGCTCTTGGAGTAGTCGGTTTAGCTTTGAACCTACGTGCCTATGACTTCGTTTCCCAGGAAATCCGTGCAGCGGAAGATCCAGAATTTGAGACTTTCTATACTAAAAATATTCTTTTAAACGAAGGTATTCGTGCTTGGATGGCGGCTCAAGATCAGCCTCATGAAAACCTTATATTCCCTGAGGAGGTTCTACCACGTGGAAACGCTCTTTAA

Protein Analysis

353

Amino Acids

39.55

Weight (kDa)

5.46

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Photo_RC PF00124 29 - 318 2.3e-73 Photosynthetic reaction centre protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 623
AciI CCGC 8 cut(s) 79, 228, 530, 623, 747, 754, 920, 992
AclI AACGTT 1 cut(s) 796
AclWI GGATC 1 cut(s) 920
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 2 cut(s) 20, 932
AcuI CTGAAG 1 cut(s) 309
AcvI CACGTG 1 cut(s) 1046
AfaI GTAC 3 cut(s) 157, 615, 825
AfiI CCNNNNNNNGG 4 cut(s) 521, 833, 919, 1033
AgeI ACCGGT 1 cut(s) 826
AgsI TTSAA 6 cut(s) 395, 413, 583, 674, 738, 877
AjnI CCWGG 1 cut(s) 903
AluBI AGCT 1 cut(s) 872
AluI AGCT 1 cut(s) 872
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 1 cut(s) 932
AlwI GGATC 1 cut(s) 920
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 2 cut(s) 230, 917
ApoI RAATTY 2 cut(s) 20, 932
AseI ATTAAT 1 cut(s) 368
AsiGI ACCGGT 1 cut(s) 826
Asp700I GAANNNNTTC 1 cut(s) 975
AspLEI GCGC 5 cut(s) 262, 359, 514, 623, 638
AspS9I GGNCC 3 cut(s) 76, 284, 446
AsuHPI GGTGA 2 cut(s) 692, 736
AvaII GGWCC 3 cut(s) 76, 284, 446
AxyI CCTNAGG 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 1046
BbsI GAAGAC 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 2 cut(s) 217, 929
BccI CCATC 4 cut(s) 592, 671, 677, 982
BceAI ACGGC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 905
BcoDI GTCTC 1 cut(s) 932
BfaI CTAG 2 cut(s) 488, 516
BfmI CTRYAG 3 cut(s) 6, 231, 654
BfoI RGCGCY 1 cut(s) 360
BisI GCNGC 5 cut(s) 231, 531, 624, 918, 993
BlsI GCNGC 5 cut(s) 232, 532, 625, 919, 994
Bme1390I CCNGG 1 cut(s) 905
Bme18I GGWCC 3 cut(s) 76, 284, 446
BmgT120I GGNCC 3 cut(s) 76, 284, 446
BmiI GGNNCC 3 cut(s) 77, 285, 448
BmrFI CCNGG 1 cut(s) 905
BmrI ACTGGG 1 cut(s) 626
BmuI ACTGGG 1 cut(s) 626
BpiI GAAGAC 1 cut(s) 463
BpuEI CTTGAG 2 cut(s) 702, 981
BsaAI YACGTR 2 cut(s) 884, 1046
BsaJI CCNNGG 4 cut(s) 13, 352, 559, 903
BsaWI WCCGGW 1 cut(s) 826
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 833, 919, 1033
Bse118I RCCGGY 2 cut(s) 608, 826
Bse1I ACTGG 4 cut(s) 68, 195, 621, 838
Bse21I CCTNAGG 1 cut(s) 1029
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 905
BseDI CCNNGG 4 cut(s) 13, 352, 559, 903
BseGI GGATG 4 cut(s) 64, 546, 849, 993
BseLI CCNNNNNNNGG 4 cut(s) 521, 833, 919, 1033
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 1020
BseNI ACTGG 4 cut(s) 68, 195, 621, 838
BseRI GAGGAG 1 cut(s) 1046
BseXI GCAGC 2 cut(s) 217, 929
BsgI GTGCAG 1 cut(s) 936
Bsh1236I CGCG 1 cut(s) 623
BshFI GGCC 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 853
BsiSI CCGG 2 cut(s) 609, 827
BslFI GGGAC 2 cut(s) 89, 432
BslI CCNNNNNNNGG 4 cut(s) 521, 833, 919, 1033
BsmAI GTCTC 1 cut(s) 932
BsmFI GGGAC 2 cut(s) 89, 432
BsmI GAATGC 2 cut(s) 262, 437
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 853
Bsp143I GATC 3 cut(s) 404, 925, 1000
BspACI CCGC 8 cut(s) 79, 228, 530, 623, 747, 754, 920, 992
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 1 cut(s) 1021
BspFNI CGCG 1 cut(s) 623
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 77, 285, 448
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 1 cut(s) 920
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 2 cut(s) 608, 826
BsrI ACTGG 4 cut(s) 68, 195, 621, 838
BssAI RCCGGY 2 cut(s) 608, 826
BssECI CCNNGG 4 cut(s) 13, 352, 559, 903
BssMI GATC 3 cut(s) 404, 925, 1000
BssNAI GTATAC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 13, 559
Bst1107I GTATAC 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 2 cut(s) 279, 655
BstBAI YACGTR 2 cut(s) 884, 1046
BstDEI CTNAG 1 cut(s) 1029
BstDSI CCRYGG 1 cut(s) 352
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 4 cut(s) 64, 546, 849, 993
BstFNI CGCG 1 cut(s) 623
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 5 cut(s) 262, 359, 514, 623, 638
BstKTI GATC 3 cut(s) 407, 928, 1003
BstMAI GTCTC 1 cut(s) 932
BstMBI GATC 3 cut(s) 404, 925, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 131, 344, 989
BstNI CCWGG 1 cut(s) 905
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 903
BstSFI CTRYAG 3 cut(s) 6, 231, 654
BstUI CGCG 1 cut(s) 623
BstV1I GCAGC 2 cut(s) 217, 929
BstV2I GAAGAC 1 cut(s) 463
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BstZ17I GTATAC 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 1029
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 4 cut(s) 64, 546, 849, 993
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 5 cut(s) 262, 359, 514, 623, 638
Cfr10I RCCGGY 2 cut(s) 608, 826
Cfr13I GGNCC 3 cut(s) 76, 284, 446
Csp6I GTAC 3 cut(s) 156, 614, 824
CspAI ACCGGT 1 cut(s) 826
CviAII CATG 4 cut(s) 174, 185, 644, 1010
CviJI RGCY 9 cut(s) 11, 194, 338, 533, 626, 722, 872, 995, 1006
CviKI_1 RGCY 9 cut(s) 11, 194, 338, 533, 626, 722, 872, 995, 1006
CviQI GTAC 3 cut(s) 156, 614, 824
DdeI CTNAG 1 cut(s) 1029
DpnI GATC 3 cut(s) 406, 927, 1002
DpnII GATC 3 cut(s) 404, 925, 1000
DraI TTTAAA 1 cut(s) 966
EaeI YGGCCR 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 13, 559
Eco47I GGWCC 3 cut(s) 76, 284, 446
Eco57I CTGAAG 1 cut(s) 309
Eco72I CACGTG 1 cut(s) 1046
Eco81I CCTNAGG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 903
EcoT14I CCWWGG 2 cut(s) 13, 559
ErhI CCWWGG 2 cut(s) 13, 559
FaeI CATG 4 cut(s) 177, 188, 647, 1013
FaqI GGGAC 2 cut(s) 89, 432
FatI CATG 4 cut(s) 173, 184, 643, 1009
FauNDI CATATG 1 cut(s) 594
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 5 cut(s) 231, 531, 624, 918, 993
FokI GGATG 4 cut(s) 71, 533, 856, 1000
Fsp4HI GCNGC 5 cut(s) 231, 531, 624, 918, 993
FspBI CTAG 2 cut(s) 488, 516
GlaI GCGC 5 cut(s) 261, 358, 513, 622, 637
GluI GCNGC 5 cut(s) 231, 531, 624, 918, 993
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 1 cut(s) 194
HapII CCGG 2 cut(s) 609, 827
HhaI GCGC 5 cut(s) 262, 359, 514, 623, 638
Hin1II CATG 4 cut(s) 177, 188, 647, 1013
Hin6I GCGC 5 cut(s) 260, 357, 512, 621, 636
HinP1I GCGC 5 cut(s) 260, 357, 512, 621, 636
HinfI GANTC 2 cut(s) 543, 603
HpaII CCGG 2 cut(s) 609, 827
HphI GGTGA 2 cut(s) 692, 736
Hpy166II GTNNAC 3 cut(s) 150, 180, 614
Hpy188III TCNNGA 4 cut(s) 287, 929, 998, 1010
Hpy8I GTNNAC 3 cut(s) 150, 180, 614
HpyAV CCTTC 2 cut(s) 203, 965
HpyCH4III ACNGT 2 cut(s) 279, 655
HpyCH4IV ACGT 4 cut(s) 385, 796, 883, 1045
HpyCH4V TGCA 5 cut(s) 216, 233, 431, 689, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 344, 989
HpyF3I CTNAG 1 cut(s) 1029
HpySE526I ACGT 4 cut(s) 385, 796, 883, 1045
Hsp92II CATG 4 cut(s) 177, 188, 647, 1013
HspAI GCGC 5 cut(s) 260, 357, 512, 621, 636
Kzo9I GATC 3 cut(s) 404, 925, 1000
Lsp1109I GCAGC 2 cut(s) 217, 929
MaeI CTAG 2 cut(s) 488, 516
MaeII ACGT 4 cut(s) 385, 796, 883, 1045
MaeIII GTNAC 8 cut(s) 66, 166, 273, 381, 742, 762, 801, 829
MalI GATC 3 cut(s) 406, 927, 1002
MboI GATC 3 cut(s) 404, 925, 1000
MboII GAAGA 4 cut(s) 463, 686, 737, 935
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 1 cut(s) 853
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 8 cut(s) 20, 217, 320, 389, 413, 477, 571, 932
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 612
MnlI CCTC 5 cut(s) 66, 560, 1017, 1024, 1027
Mox20I TGGCCA 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 975
MscI TGGCCA 1 cut(s) 194
MseI TTAA 5 cut(s) 224, 368, 708, 965, 1060
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 2 cut(s) 230, 920
MspI CCGG 2 cut(s) 609, 827
MspR9I CCNGG 1 cut(s) 905
MunI CAATTG 1 cut(s) 413
Mva1269I GAATGC 2 cut(s) 262, 437
MvaI CCWGG 1 cut(s) 905
MvnI CGCG 1 cut(s) 623
MwoI GCNNNNNNNGC 3 cut(s) 131, 344, 989
NdeI CATATG 1 cut(s) 594
NdeII GATC 3 cut(s) 404, 925, 1000
NlaIII CATG 4 cut(s) 177, 188, 647, 1013
NlaIV GGNNCC 3 cut(s) 77, 285, 448
NmuCI GTSAC 2 cut(s) 742, 762
PagI TCATGA 1 cut(s) 1009
PctI GAATGC 2 cut(s) 262, 437
PdmI GAANNNNTTC 1 cut(s) 975
PfeI GAWTC 1 cut(s) 543
PinAI ACCGGT 1 cut(s) 826
PkrI GCNGC 5 cut(s) 232, 532, 625, 919, 994
PleI GAGTC 1 cut(s) 611
PmaCI CACGTG 1 cut(s) 1046
PmlI CACGTG 1 cut(s) 1046
PpsI GAGTC 1 cut(s) 611
Ppu21I YACGTR 2 cut(s) 884, 1046
PpuMI RGGWCCY 1 cut(s) 284
PshBI ATTAAT 1 cut(s) 368
PsiI TTATAA 1 cut(s) 426
Psp1406I AACGTT 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 903
PspCI CACGTG 1 cut(s) 1046
PspEI GGTNACC 1 cut(s) 742
PspGI CCWGG 1 cut(s) 903
PspN4I GGNNCC 3 cut(s) 77, 285, 448
PspPI GGNCC 3 cut(s) 76, 284, 446
PspPPI RGGWCCY 1 cut(s) 284
PstI CTGCAG 1 cut(s) 235
PsuI RGATCY 1 cut(s) 925
RsaI GTAC 3 cut(s) 157, 615, 825
RsaNI GTAC 3 cut(s) 156, 614, 824
SaqAI TTAA 5 cut(s) 224, 368, 708, 965, 1060
SatI GCNGC 5 cut(s) 231, 531, 624, 918, 993
Sau3AI GATC 3 cut(s) 404, 925, 1000
Sau96I GGNCC 3 cut(s) 76, 284, 446
SchI GAGTC 1 cut(s) 612
ScrFI CCNGG 1 cut(s) 905
SduI GDGCHC 1 cut(s) 853
SfcI CTRYAG 3 cut(s) 6, 231, 654
SgrAI CRCCGGYG 1 cut(s) 608
SinI GGWCC 3 cut(s) 76, 284, 446
SmlI CTYRAG 2 cut(s) 717, 996
SmoI CTYRAG 2 cut(s) 717, 996
Sse9I AATT 8 cut(s) 20, 217, 320, 389, 413, 477, 571, 932
SsiI CCGC 8 cut(s) 79, 228, 530, 623, 747, 754, 920, 992
SspI AATATT 1 cut(s) 958
SspMI CTAG 2 cut(s) 488, 516
StyD4I CCNGG 1 cut(s) 903
StyI CCWWGG 2 cut(s) 13, 559
TaaI ACNGT 2 cut(s) 279, 655
TaiI ACGT 4 cut(s) 388, 799, 886, 1048
TaqI TCGA 2 cut(s) 403, 541
TasI AATT 8 cut(s) 20, 217, 320, 389, 413, 477, 571, 932
TatI WGTACW 1 cut(s) 613
TauI GCSGC 3 cut(s) 533, 626, 995
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 5 cut(s) 224, 368, 708, 965, 1060
Tru9I TTAA 5 cut(s) 224, 368, 708, 965, 1060
TseFI GTSAC 2 cut(s) 742, 762
TseI GCWGC 2 cut(s) 230, 917
Tsp45I GTSAC 2 cut(s) 742, 762
TspDTI ATGAA 6 cut(s) 162, 535, 557, 581, 632, 1026
TspGWI ACGGA 3 cut(s) 85, 689, 902
VpaK11BI GGWCC 3 cut(s) 76, 284, 446
VspI ATTAAT 1 cut(s) 368
XapI RAATTY 2 cut(s) 20, 932
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 975
XspI CTAG 2 cut(s) 488, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.