RorugMtG0002000

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors. D2 is needed for assembly of a stable PSII complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000009
OG0004028
Physical Location & Seq
Reverse (-)
123249 .. 125040
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugMtG0002000.1

Sequence Viewer

Length: 951 bp
ATGCCTGTCAATCGAGTAGGTGATTTTGGATTAGCTCCTGGGATTTTGGGCCGTTTTACTCTCTTTCAAACAGTAGACTTTTCAACCATTTTTGCTCGTGCTAGTGCCCCCAGAAATTCTTGGATTTCTTGCAATATGAGATTTAATGCCATAACTCTTATTTGTATTTTACTTTTTATTGGTGCTGTTGGGAAATCTGCACAGATAGGATTGCATACTTGGTCACCCGATGCTATGGAGGGTCCCACTCCAGTATCCGCTTCGATTCATGCAGCTACTATGGTAACAGCTGGCGTTTTTATGATAGCAAGGTGCTCCCCTTTATTTGAATACCCACCTACGGCTTTGATTGTTATTACTTCTGCAGGAGCTACGACGTCATTCCTTGCGGCAACCACTGGAATATTACAGAACGATCTAAAGAGGGTCATAGCTTATTCAACTTGCAGTCAATTAGGCTATATGATCTTTGCTTGCGGCATCTCTAACTATTCGGTTAGCGTCTTTCACTTAATGAATCACGCGTTTTTCAAAGCATTACTATTCCTGAGTGCAGGTTCGGTGATTCATGCCATGTCGGATGAGCAAGATATGCGGAAGATGGGAGGGCTTGCCTCCTCGTTCCCTTTTACCTATGCCATGATGCTCATGGGCAGCTTATCTCTAATTGGATTCCCTTTTCTAACTGGATTTTATTCCAAAGATGTGATCTTAGAGCTCGCTTACACTAAGTATACCATCAGTGGGAACTTTGCTTTCTGGTTGGGAAGTGTCTCTGTCCTTTTCACTTCTTATTACTCCTTTCGTTTACTTTTTCTAACATTTCTAGTACCAACAAATTCATTCGGGCGAGACATCTTACGATGTCATGATGCGCCCATTCCTATGGCCATTCCTTCAATACTTCTGGCTCTCGGGAGTCTCTTTGTAGGATACTTGGCCAAAGTGTGA

Protein Analysis

316

Amino Acids

34.29

Weight (kDa)

8.9

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 3 - 254 7.5e-62 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 380
Acc36I ACCTGC 1 cut(s) 545
AccI GTMKAC 2 cut(s) 75, 734
AccII CGCG 1 cut(s) 524
AciI CCGC 4 cut(s) 258, 389, 477, 595
AcoI YGGCCR 2 cut(s) 888, 939
AcsI RAATTY 2 cut(s) 115, 838
AcyI GRCGYC 1 cut(s) 377
AfaI GTAC 1 cut(s) 831
AfiI CCNNNNNNNGG 2 cut(s) 340, 744
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 6 cut(s) 68, 84, 329, 441, 532, 900
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 7 cut(s) 35, 275, 290, 371, 434, 657, 718
AluI AGCT 7 cut(s) 35, 275, 290, 371, 434, 657, 718
Alw21I GWGCWC 2 cut(s) 317, 720
Alw26I GTCTC 3 cut(s) 778, 846, 926
Ama87I CYCGRG 1 cut(s) 914
AoxI GGCC 3 cut(s) 49, 888, 939
ApeKI GCWGC 2 cut(s) 272, 654
ApoI RAATTY 2 cut(s) 115, 838
AspLEI GCGC 1 cut(s) 877
AspS9I GGNCC 2 cut(s) 49, 242
AsuHPI GGTGA 3 cut(s) 32, 216, 574
AvaI CYCGRG 1 cut(s) 914
AvaII GGWCC 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 109
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BalI TGGCCA 2 cut(s) 890, 941
BanII GRGCYC 1 cut(s) 720
BauI CACGAG 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 317, 720
BbvI GCAGC 2 cut(s) 284, 666
BccI CCATC 2 cut(s) 595, 746
BceAI ACGGC 2 cut(s) 36, 357
BciT130I CCWGG 1 cut(s) 39
BciVI GTATCC 2 cut(s) 265, 926
BcoDI GTCTC 3 cut(s) 778, 846, 926
BfaI CTAG 2 cut(s) 102, 827
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 545
BfuI GTATCC 2 cut(s) 265, 926
BisI GCNGC 4 cut(s) 273, 390, 478, 655
BlsI GCNGC 4 cut(s) 274, 391, 479, 656
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 242
BmeT110I CYCGRG 1 cut(s) 914
BmgT120I GGNCC 2 cut(s) 49, 242
BmiI GGNNCC 2 cut(s) 243, 244
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 4 cut(s) 220, 489, 633, 862
BpmI CTGGAG 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 38
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 744
Bse1I ACTGG 3 cut(s) 251, 403, 691
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 340, 744
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 3 cut(s) 251, 403, 691
BseRI GAGGAG 1 cut(s) 607
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 2 cut(s) 284, 666
BsgI GTGCAG 2 cut(s) 183, 573
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 3 cut(s) 51, 890, 941
BsiHKAI GWGCWC 2 cut(s) 317, 720
BsiHKCI CYCGRG 1 cut(s) 914
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 340, 744
BsmAI GTCTC 3 cut(s) 778, 846, 926
BsmFI GGGAC 1 cut(s) 228
BsnI GGCC 3 cut(s) 51, 890, 941
BsoBI CYCGRG 1 cut(s) 914
Bsp1286I GDGCHC 3 cut(s) 109, 317, 720
Bsp143I GATC 3 cut(s) 415, 465, 708
BspACI CCGC 4 cut(s) 258, 389, 477, 595
BspANI GGCC 3 cut(s) 51, 890, 941
BspCNI CTCAG 1 cut(s) 540
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 2 cut(s) 243, 244
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 545
BsrI ACTGG 3 cut(s) 251, 403, 691
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 3 cut(s) 415, 465, 708
BssNAI GTATAC 1 cut(s) 735
BssNI GRCGYC 1 cut(s) 377
BssSI CACGAG 1 cut(s) 96
Bst1107I GTATAC 1 cut(s) 735
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 592
BstC8I GCNNGC 4 cut(s) 292, 475, 612, 720
BstDEI CTNAG 3 cut(s) 548, 712, 729
BstEII GGTNACC 1 cut(s) 222
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 1 cut(s) 524
BstHHI GCGC 1 cut(s) 877
BstKTI GATC 3 cut(s) 418, 468, 711
BstMAI GTCTC 3 cut(s) 778, 846, 926
BstMBI GATC 3 cut(s) 415, 465, 708
BstMWI GCNNNNNNNGC 1 cut(s) 592
BstNI CCWGG 1 cut(s) 39
BstPI GGTNACC 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 37
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 109
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 2 cut(s) 284, 666
BstXI CCANNNNNNTGG 1 cut(s) 886
BstZ17I GTATAC 1 cut(s) 735
BsuI GTATCC 2 cut(s) 265, 926
BsuRI GGCC 3 cut(s) 51, 890, 941
BtsCI GGATG 1 cut(s) 586
BtsIMutI CAGTG 2 cut(s) 396, 748
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 4 cut(s) 292, 475, 612, 720
CciI TCATGA 1 cut(s) 868
CfoI GCGC 1 cut(s) 877
Cfr13I GGNCC 2 cut(s) 49, 242
CseI GACGC 1 cut(s) 490
Csp6I GTAC 1 cut(s) 830
CviAII CATG 6 cut(s) 269, 569, 574, 640, 649, 869
CviQI GTAC 1 cut(s) 830
DdeI CTNAG 3 cut(s) 548, 712, 729
DpnI GATC 3 cut(s) 417, 467, 710
DpnII GATC 3 cut(s) 415, 465, 708
EaeI YGGCCR 2 cut(s) 888, 939
Ecl136II GAGCTC 1 cut(s) 718
Eco24I GRGCYC 1 cut(s) 720
Eco47I GGWCC 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 718
Eco88I CYCGRG 1 cut(s) 914
Eco91I GGTNACC 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 718
EcoO109I RGGNCCY 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 720
FaeI CATG 6 cut(s) 272, 572, 577, 643, 652, 872
FaqI GGGAC 1 cut(s) 228
FatI CATG 6 cut(s) 268, 568, 573, 639, 648, 868
FblI GTMKAC 2 cut(s) 75, 734
Fnu4HI GCNGC 4 cut(s) 273, 390, 478, 655
FokI GGATG 1 cut(s) 593
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 4 cut(s) 273, 390, 478, 655
FspBI CTAG 2 cut(s) 102, 827
GlaI GCGC 1 cut(s) 876
GluI GCNGC 4 cut(s) 273, 390, 478, 655
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 3 cut(s) 51, 890, 941
HgaI GACGC 1 cut(s) 490
HhaI GCGC 1 cut(s) 877
Hin1I GRCGYC 1 cut(s) 377
Hin1II CATG 6 cut(s) 272, 572, 577, 643, 652, 872
Hin6I GCGC 1 cut(s) 875
HinP1I GCGC 1 cut(s) 875
HinfI GANTC 5 cut(s) 265, 517, 565, 672, 919
HphI GGTGA 3 cut(s) 32, 216, 574
Hpy166II GTNNAC 3 cut(s) 76, 735, 809
Hpy188I TCNGA 1 cut(s) 580
Hpy188III TCNNGA 3 cut(s) 547, 869, 916
Hpy8I GTNNAC 3 cut(s) 76, 735, 809
Hpy99I CGWCG 1 cut(s) 379
HpyAV CCTTC 1 cut(s) 906
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 7 cut(s) 132, 200, 214, 272, 365, 447, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 592
HpyF3I CTNAG 3 cut(s) 548, 712, 729
HpySE526I ACGT 1 cut(s) 377
Hsp92I GRCGYC 1 cut(s) 377
Hsp92II CATG 6 cut(s) 272, 572, 577, 643, 652, 872
HspAI GCGC 1 cut(s) 875
KflI GGGWCCC 1 cut(s) 242
Kzo9I GATC 3 cut(s) 415, 465, 708
LmnI GCTCC 3 cut(s) 40, 320, 368
Lsp1109I GCAGC 2 cut(s) 284, 666
LweI GCATC 4 cut(s) 220, 489, 633, 862
MaeI CTAG 2 cut(s) 102, 827
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 2 cut(s) 222, 283
MalI GATC 3 cut(s) 417, 467, 710
MboI GATC 3 cut(s) 415, 465, 708
MboII GAAGA 1 cut(s) 610
MhlI GDGCHC 3 cut(s) 109, 317, 720
MlsI TGGCCA 2 cut(s) 890, 941
MluCI AATT 4 cut(s) 115, 452, 666, 838
MluI ACGCGT 1 cut(s) 522
MluNI TGGCCA 2 cut(s) 890, 941
MlyI GAGTC 1 cut(s) 928
MmeI TCCRAC 1 cut(s) 558
MnlI CCTC 5 cut(s) 232, 417, 599, 625, 628
Mox20I TGGCCA 2 cut(s) 890, 941
MscI TGGCCA 2 cut(s) 890, 941
MseI TTAA 2 cut(s) 144, 512
MslI CAYNNNNRTG 1 cut(s) 884
Msp20I TGGCCA 2 cut(s) 890, 941
MspA1I CMGCKG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 39
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 1 cut(s) 592
NdeII GATC 3 cut(s) 415, 465, 708
NlaIII CATG 6 cut(s) 272, 572, 577, 643, 652, 872
NlaIV GGNNCC 2 cut(s) 243, 244
NmuCI GTSAC 1 cut(s) 222
PagI TCATGA 1 cut(s) 868
PfeI GAWTC 4 cut(s) 265, 517, 565, 672
PkrI GCNGC 4 cut(s) 274, 391, 479, 656
PleI GAGTC 1 cut(s) 927
PpsI GAGTC 1 cut(s) 927
PpuMI RGGWCCY 1 cut(s) 242
Psp124BI GAGCTC 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 37
PspEI GGTNACC 1 cut(s) 222
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 243, 244
PspPI GGNCC 2 cut(s) 49, 242
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 831
RsaNI GTAC 1 cut(s) 830
RseI CAYNNNNRTG 1 cut(s) 884
SacI GAGCTC 1 cut(s) 720
SaqAI TTAA 2 cut(s) 144, 512
SatI GCNGC 4 cut(s) 273, 390, 478, 655
Sau3AI GATC 3 cut(s) 415, 465, 708
Sau96I GGNCC 2 cut(s) 49, 242
SchI GAGTC 1 cut(s) 928
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 3 cut(s) 109, 317, 720
SfaNI GCATC 4 cut(s) 220, 489, 633, 862
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 884
Sse9I AATT 4 cut(s) 115, 452, 666, 838
SsiI CCGC 4 cut(s) 258, 389, 477, 595
SspI AATATT 1 cut(s) 405
SspMI CTAG 2 cut(s) 102, 827
SstI GAGCTC 1 cut(s) 720
StyD4I CCNGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 380
TaqI TCGA 2 cut(s) 13, 263
TasI AATT 4 cut(s) 115, 452, 666, 838
TauI GCSGC 2 cut(s) 392, 480
TfiI GAWTC 4 cut(s) 265, 517, 565, 672
Tru1I TTAA 2 cut(s) 144, 512
Tru9I TTAA 2 cut(s) 144, 512
TscAI CASTG 2 cut(s) 403, 748
TseFI GTSAC 1 cut(s) 222
TseI GCWGC 2 cut(s) 272, 654
Tsp45I GTSAC 1 cut(s) 222
TspDTI ATGAA 4 cut(s) 257, 530, 557, 831
TspRI CASTG 2 cut(s) 403, 748
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 2 cut(s) 115, 838
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 2 cut(s) 75, 734
XspI CTAG 2 cut(s) 102, 827
ZraI GACGTC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.