RCHIOBHM_CPG0502321

One of the components of the core complex of photosystem II (PSII). It binds chlorophyll and helps catalyze the primary light-induced photochemical processes of PSII. PSII is a light- driven water plastoquinone oxidoreductase, using light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0004028
Physical Location & Seq
Reverse (-)
77055 .. 78025
971 bp
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UTR
Exon/CDS
Intron
PRQ15712

Sequence Viewer

Length: 576 bp
ATGGGCTCGTCGTGCACTTGTATGGTCTGGAGAGGCTTACCTGTCTTATATGAGTTTTACGGGCCCACTGGACCCGAAGCTTCTCAAGCTCAAGCATTTACTTTTTTGGTTAGAGACCAACGTCTTGGGGCTAACGTAGGATCTGCTCAAGGACCTACCGGTTTAGGTAAATATCTAATGCGCTCTCCAACTGGGGAAGTTATTTTTGGAGGAGAAACTATGCGTTTCTGGGATCTGCGTGCCCCCTGGTTAGAACCTCTAAGGGGTCCTAATGGTTTGGACTTGAGTAGGCTGAAAAAAGACATACAGCCTTGGCAAGAGCGGCGTTCTGCGGAATATATGACTCATGCTCCTTTAGGTTCTTTAAATTCCGTGGGTGGCGTAGCTACCGAGATCAATGCAGTCAATTATGTCTCTCCTAGAAGTTGGTTATCTACTTCTCATTTTGTTCTAGGATTCTTCCTATTCGTAGGTCATTTATGGCATGCAGGAAGGGCTCGTGCAGCTGCTGCCGGATTTGAAAAAGGAATTGATCGTGATTTTGAACCTGTTCTTTCCATGACTCCTCTTAACTGA

Protein Analysis

191

Amino Acids

21.04

Weight (kDa)

8.66

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII PF00421 26 - 187 8.2e-46 Photosystem II protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 322
AciI CCGC 2 cut(s) 322, 332
AclWI GGATC 2 cut(s) 148, 240
AcsI RAATTY 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 263
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 2 cut(s) 521, 545
AjnI CCWGG 1 cut(s) 245
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 4 cut(s) 80, 89, 386, 506
AluI AGCT 4 cut(s) 80, 89, 386, 506
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 2 cut(s) 108, 418
Alw44I GTGCAC 1 cut(s) 13
AlwI GGATC 2 cut(s) 148, 240
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 1 cut(s) 62
ApaI GGGCCC 1 cut(s) 66
ApaLI GTGCAC 1 cut(s) 13
ApeKI GCWGC 3 cut(s) 503, 506, 509
ApoI RAATTY 1 cut(s) 367
AsiGI ACCGGT 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 5 cut(s) 62, 63, 71, 152, 266
AvaII GGWCC 3 cut(s) 71, 152, 266
BaeGI GKGCMC 3 cut(s) 17, 66, 244
BanII GRGCYC 3 cut(s) 8, 66, 499
BauI CACGAG 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 493, 496, 515
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 108, 418
BfaI CTAG 2 cut(s) 420, 452
BisI GCNGC 4 cut(s) 323, 504, 507, 510
BlsI GCNGC 4 cut(s) 324, 505, 508, 511
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 3 cut(s) 71, 152, 266
BmgT120I GGNCC 5 cut(s) 62, 63, 71, 152, 266
BmiI GGNNCC 3 cut(s) 64, 73, 267
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 201
BoxI GACNNNNGTC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 49
BpuEI CTTGAG 4 cut(s) 69, 75, 132, 304
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 245, 311, 372
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 73, 196
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 3 cut(s) 245, 311, 372
BseLI CCNNNNNNNGG 1 cut(s) 263
BseNI ACTGG 2 cut(s) 73, 196
BseRI GAGGAG 2 cut(s) 225, 555
BseSI GKGCMC 3 cut(s) 17, 66, 244
BseXI GCAGC 3 cut(s) 493, 496, 515
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 1 cut(s) 64
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 2 cut(s) 159, 513
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 108, 418
BsnI GGCC 1 cut(s) 64
Bso31I GGTCTC 1 cut(s) 108
Bsp120I GGGCCC 1 cut(s) 62
Bsp1286I GDGCHC 5 cut(s) 8, 17, 66, 244, 499
Bsp143I GATC 4 cut(s) 140, 232, 393, 532
BspACI CCGC 2 cut(s) 322, 332
BspANI GGCC 1 cut(s) 64
BspLI GGNNCC 3 cut(s) 64, 73, 267
BspPI GGATC 2 cut(s) 148, 240
BspTNI GGTCTC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 73, 196
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 3 cut(s) 245, 311, 372
BssMI GATC 4 cut(s) 140, 232, 393, 532
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 240, 486
BstDEI CTNAG 1 cut(s) 260
BstDSI CCRYGG 1 cut(s) 372
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 4 cut(s) 143, 235, 396, 535
BstMAI GTCTC 2 cut(s) 108, 418
BstMBI GATC 4 cut(s) 140, 232, 393, 532
BstMWI GCNNNNNNNGC 6 cut(s) 12, 86, 322, 494, 503, 509
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 488
BstPAI GACNNNNGTC 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 245
BstSLI GKGCMC 3 cut(s) 17, 66, 244
BstV1I GCAGC 3 cut(s) 493, 496, 515
BstX2I RGATCY 2 cut(s) 140, 232
BstXI CCANNNNNNTGG 1 cut(s) 125
BstYI RGATCY 2 cut(s) 140, 232
BsuRI GGCC 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 240, 486
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 5 cut(s) 62, 63, 71, 152, 266
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 3 cut(s) 347, 485, 559
DdeI CTNAG 1 cut(s) 260
DpnI GATC 4 cut(s) 142, 234, 395, 534
DpnII GATC 4 cut(s) 140, 232, 393, 532
DraI TTTAAA 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 3 cut(s) 8, 66, 499
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 3 cut(s) 71, 152, 266
EcoO109I RGGNCCY 2 cut(s) 152, 266
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 311
EcoT38I GRGCYC 3 cut(s) 8, 66, 499
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 3 cut(s) 350, 488, 562
FatI CATG 3 cut(s) 346, 484, 558
Fnu4HI GCNGC 4 cut(s) 323, 504, 507, 510
FriOI GRGCYC 3 cut(s) 8, 66, 499
Fsp4HI GCNGC 4 cut(s) 323, 504, 507, 510
FspBI CTAG 2 cut(s) 420, 452
GlaI GCGC 1 cut(s) 182
GluI GCNGC 4 cut(s) 323, 504, 507, 510
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 1 cut(s) 64
HapII CCGG 2 cut(s) 159, 513
HhaI GCGC 1 cut(s) 183
Hin1II CATG 3 cut(s) 350, 488, 562
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 3 cut(s) 343, 456, 562
HpaII CCGG 2 cut(s) 159, 513
Hpy166II GTNNAC 1 cut(s) 15
Hpy188III TCNNGA 2 cut(s) 28, 536
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 486
HpyCH4IV ACGT 2 cut(s) 121, 135
HpyCH4V TGCA 4 cut(s) 15, 401, 488, 503
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 86, 322, 494, 503, 509
HpyF3I CTNAG 1 cut(s) 260
HpySE526I ACGT 2 cut(s) 121, 135
Hsp92II CATG 3 cut(s) 350, 488, 562
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 4 cut(s) 140, 232, 393, 532
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 3 cut(s) 493, 496, 515
MaeI CTAG 2 cut(s) 420, 452
MaeII ACGT 2 cut(s) 121, 135
MalI GATC 4 cut(s) 142, 234, 395, 534
MbiI CCGCTC 1 cut(s) 322
MboI GATC 4 cut(s) 140, 232, 393, 532
MboII GAAGA 1 cut(s) 451
MflI RGATCY 2 cut(s) 140, 232
MhlI GDGCHC 5 cut(s) 8, 17, 66, 244, 499
MluCI AATT 3 cut(s) 367, 406, 528
MlyI GAGTC 2 cut(s) 337, 556
MmeI TCCRAC 1 cut(s) 212
MnlI CCTC 4 cut(s) 26, 203, 267, 576
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 2 cut(s) 365, 570
MslI CAYNNNNRTG 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 2 cut(s) 159, 513
MspR9I CCNGG 1 cut(s) 247
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 6 cut(s) 12, 86, 322, 494, 503, 509
NdeII GATC 4 cut(s) 140, 232, 393, 532
NlaIII CATG 3 cut(s) 350, 488, 562
NlaIV GGNNCC 3 cut(s) 64, 73, 267
NspI RCATGY 1 cut(s) 488
PaeI GCATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 4 cut(s) 324, 505, 508, 511
PleI GAGTC 2 cut(s) 337, 556
PpsI GAGTC 2 cut(s) 337, 556
PpuMI RGGWCCY 2 cut(s) 152, 266
PshAI GACNNNNGTC 1 cut(s) 120
Psp5II RGGWCCY 2 cut(s) 152, 266
Psp6I CCWGG 1 cut(s) 245
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 3 cut(s) 64, 73, 267
PspOMI GGGCCC 1 cut(s) 62
PspPI GGNCC 5 cut(s) 62, 63, 71, 152, 266
PspPPI RGGWCCY 2 cut(s) 152, 266
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 2 cut(s) 140, 232
PvuII CAGCTG 1 cut(s) 506
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 2 cut(s) 365, 570
SatI GCNGC 4 cut(s) 323, 504, 507, 510
Sau3AI GATC 4 cut(s) 140, 232, 393, 532
Sau96I GGNCC 5 cut(s) 62, 63, 71, 152, 266
SchI GAGTC 2 cut(s) 337, 556
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 5 cut(s) 8, 17, 66, 244, 499
SinI GGWCC 3 cut(s) 71, 152, 266
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 4 cut(s) 84, 90, 147, 283
SmoI CTYRAG 4 cut(s) 84, 90, 147, 283
SphI GCATGC 1 cut(s) 488
Sse9I AATT 3 cut(s) 367, 406, 528
SsiI CCGC 2 cut(s) 322, 332
SspMI CTAG 2 cut(s) 420, 452
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 311
TaiI ACGT 2 cut(s) 124, 138
TasI AATT 3 cut(s) 367, 406, 528
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 1 cut(s) 456
Tru1I TTAA 2 cut(s) 365, 570
Tru9I TTAA 2 cut(s) 365, 570
TscAI CASTG 1 cut(s) 73
TseI GCWGC 3 cut(s) 503, 506, 509
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 1 cut(s) 73
VneI GTGCAC 1 cut(s) 13
VpaK11BI GGWCC 3 cut(s) 71, 152, 266
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 488
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 2 cut(s) 420, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.