Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0002170

Physical Location & Seq

Genomic Coordinates Reverse (-)

429708 .. 434675

Gene Structure 4968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g00870.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGTTTGACTGTGGCTACACGTCACAGTACGGTGGAGGGCAGAGAGACAAGTTTGTGAGGCTGGATGACTTGGACTCTCGATTATCGTCATCTTCTGATACAGGAGTGAGAAGGTGTGGACTTAATATTGAAGGACTGACCCGGAGTGCTCCGACAAGGGATACGTCCTTTAAAAAAGGAATGAGAAAGGGGTCTGAAGGACTCAAGTCCATTGGTCGATCACTTGGATTTGGTGTTTCTCGTGCAGTGTTCCCTGAAGATCTTAATGCTTCTGACAAGAAGATATTTGACCCTCAGGACAAATTCCTCCTTTTTTGGAACAAACTTTTCGTCATATCATGTATTCTGGCGGTTTCTGTGGATCCTCTTTTTTTCTACCTTCCAGTTATCAATAATGCATCAAACTGTCTTGGTATAGACCGAAAGTTGGCTGTCATAGCAACCACATTACGAATGATTGTTGATGCTTTCTTCCTTCTTCACATGGCTCTCCAGTTTCGGACAGCCTACATTGCTCCATCATCTCGGGTTTTTGGACGAGGTGAACTGGTGATTGACCCAGCAAGAATAGCCAAGCGATACTTACGGGGTTATTTCATCATTGATTTTCTTTCTGTACTACCTCTACCACAGATTGTAGTTTGGAGGTTTCTGCAGCGTTCTGGAGGTTCAGATGTGCTTGCTACAAAACAGGCTTTGCTTTTCATTGTCTTACTTCAGTACATTCCAAGACTTCTTCGAGTCTTGCCTTTAACCTCCGAGCTGAAAAGAACAGCTGGTACTTTTGCTGAAACTGCTTGGGCCGGTGCTGCGTACTATTTGCTATTGTACTTGCTTGCTAGTCATATTGTTGGGGCATTTTGGTATTTATTGGCTGTAGAACGCAATGATACATGCTGGCAGAAGGCTTGTACTGACATTGGCAGTGCATGCGATAAAAGATTATTATACTGTGCGGATGAGAATCTGCCAACAGCTGAGAACTGGTCCAACATCAGTGGTATAATCCAGTCAAATTGCTCATCGGCTGATGATAATAAGTATTTTGATTTCGGAATCTACCAACAGGCTTTATCATCTAGAATTGTTTCATCTAAGAAGTTCATCTCTAAGTACTGTTACTGTTTATGGTGGGGGTTACAAAATTTAAGTACACTTGGCCAGGGGCTCTCAACTAGCACCTATCCCGGAGAGGTTATATTTTCCATAGCGCTAGCTATATTTGGATTGATCCTCTTTGCACTTCTGATAGGAAATATGCAGACCTACCTCCAGTCTCTTACCATTCGACTTGAGGAGATGAGAGTTAAAAGGCGTGATTCAGAGCAGTGGATGCACCATCGCTTACTTCCACAAGACCTCCGGGAGCGAGTTAGGAGATATGATCAATATAAATGGTTGGAAACACGTGGAGTGGATGAGGAGAATTTGGTTCAGAGTCTACCAAAGGATCTCAGGAGAGATATCAAGCGGCACCTCTGTTTGGCGTTAGTGAGGAGGGTTCCTCTGTTTGAGAATATGGATGAGAGGTTGCTTGATGCCATCTGCGAGCGACTGAAACCAAGTTTGTTCACACAGAGTACCTACATAGTTCGAGAAGGAGATCCAGTTGACGAAATGCTTTTCATCATACGTGGACGCCTTGAGAGTGTGACCACAGATGGTGGCAGGAGTGGTTTTTTCAACCGCAGTCTGCTAAAAGAAGGGGATTTCTGTGGGGAGGAGCTTCTAACCTGGGCACTGGATCCCAAATCTGGTTCAAACCTTCCTTCATCTACAAGGACTGTGAAGGCATTAGGAGAGGTCGAGGCTTTTGCCCTTATAGCTGAAGAGCTGAAATTTGTTGCCAGTCAGTTTAGGCGCCTTCATAGCAGGCAGGTTCAACACACCTTCCGTTTCTACTCGCAACAGTGGCGTACATGGGCTGCTTGCTTCATCCAAGCGGCATGGCGTCGCTATTCCAAGAGGAAAATCATGGAGCTTCGCCGGAAGGAAGAGGAAGAAGCAGAAACAGAAGCACTGGCAGGTGCCCGCCACAATGTTAGTATTGGTGCCACCTTTTTAGCTACTAGGTTTGCTGCAAATGCACTTCGCGGTGTTCATCGAAATAGGAATGCAAAGATTGCTCAGAGCGCTCGAGATTTGGTCAAATTACAAAAGCCTCCAGAGCCCGATTTTAGTGCTGAAGATGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005217 GO:0005221 GO:0005223 GO:0005244 GO:0005249 GO:0005261 GO:0005262 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006816 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015077 GO:0015079 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042391 GO:0043855 GO:0044425 GO:0044459 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071804 GO:0071805 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

733

Amino Acids

83.41

Weight (kDa)

9.24

Isoelectric Point (pI)

50.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	105 - 429	3.7e-25	Ion transport protein
cNMP_binding	PF00027	524 - 612	1.6e-06	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002170)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT4G30560 AT4G30560 AT4G30560 AT4G30560 AT4G30560 AT5G57940 AT5G57940 AT5G57940
fragaria_vesca	FvH4_1g00870 FvH4_1g00870 FvH4_1g00870 FvH4_1g00870
malus_domestica	MD02G1007200.v1.1 MD15G1144500.v1.1
prunus_persica	Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1
pyrus_communis	pycom02g00610 pycom02g00620 pycom15g13600 pycom15g13610
rosa_chinensis	RchiOBHm_Chr2g0085581
rosa_laevigata	RLG00000015703
rosa_multiflora	Rmu_co8217668.1_g000001 Rmu_ssc0000347.1_g000007
rosa_roxburghii	Rroxscaffold_2G00155250
rosa_rugosa	Rorug01G0461400
rosa_samantha	Rh2AG010500 Rh2BG011100 Rh2CG012000 Rh2DG013000
rosa_wichuraiana	Rw2G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2015
Acc36I	ACCTGC	2 cut(s)	1867, 2015
AccB1I	GGYRCC	4 cut(s)	1473, 1860, 2027, 2051
AccI	GTMKAC	1 cut(s)	1441
AccII	CGCG	1 cut(s)	2094
AciI	CCGC	7 cut(s)	350, 956, 1471, 1687, 1943, 2032, 2094
AclWI	GGATC	7 cut(s)	356, 369, 1225, 1458, 1598, 1739, 1752
AcoI	YGGCCR	1 cut(s)	1159
AcsI	RAATTY	4 cut(s)	302, 1144, 1426, 1838
AcuI	CTGAAG	4 cut(s)	216, 276, 701, 1848
AcvI	CACGTG	1 cut(s)	1409
AcyI	GRCGYC	3 cut(s)	1639, 1861, 1951
AfeI	AGCGCT	2 cut(s)	1212, 2134
AfiI	CCNNNNNNNGG	3 cut(s)	427, 1483, 1754
AflIII	ACRYGT	2 cut(s)	18, 1406
AgsI	TTSAA	4 cut(s)	131, 1684, 1761, 1883
AjiI	CACGTC	1 cut(s)	21
AjnI	CCWGG	2 cut(s)	1161, 1733
AluBI	AGCT	9 cut(s)	763, 776, 977, 1217, 1726, 1826, 1834, 1981, 2066
AluI	AGCT	9 cut(s)	763, 776, 977, 1217, 1726, 1826, 1834, 1981, 2066
Alw21I	GWGCWC	1 cut(s)	151
Alw26I	GTCTC	2 cut(s)	39, 1281
AlwI	GGATC	7 cut(s)	356, 369, 1225, 1458, 1598, 1739, 1752
Ama87I	CYCGRG	2 cut(s)	525, 2136
Aor51HI	AGCGCT	2 cut(s)	1212, 2134
AoxI	GGCC	2 cut(s)	801, 1159
ApeKI	GCWGC	4 cut(s)	655, 809, 1925, 2078
ApoI	RAATTY	4 cut(s)	302, 1144, 1426, 1838
ArsI	GACNNNNNNTTYG	2 cut(s)	315, 347
Asp700I	GAANNNNTTC	1 cut(s)	1087
AspLEI	GCGC	3 cut(s)	1213, 1863, 2135
AspS9I	GGNCC	2 cut(s)	801, 987
AsuC2I	CCSGG	3 cut(s)	142, 1188, 1364
AsuHPI	GGTGA	2 cut(s)	554, 562
AsuNHI	GCTAGC	1 cut(s)	1213
AvaI	CYCGRG	2 cut(s)	525, 2136
AvaII	GGWCC	1 cut(s)	987
AxyI	CCTNAGG	1 cut(s)	294
BaeGI	GKGCMC	2 cut(s)	1741, 2032
BaeI	ACNNNNGTAYC	2 cut(s)	90, 123
BalI	TGGCCA	1 cut(s)	1161
BamHI	GGATCC	2 cut(s)	361, 1744
BanI	GGYRCC	4 cut(s)	1473, 1860, 2027, 2051
BanII	GRGCYC	2 cut(s)	1170, 2172
BauI	CACGAG	1 cut(s)	240
BbrPI	CACGTG	1 cut(s)	1409
Bbv12I	GWGCWC	1 cut(s)	151
BbvI	GCAGC	4 cut(s)	667, 796, 1912, 2065
BccI	CCATC	4 cut(s)	526, 1347, 1550, 1655
BcgI	CGANNNNNNTGC	4 cut(s)	1796, 1830, 2162, 2196
BciT130I	CCWGG	2 cut(s)	1163, 1735
BciVI	GTATCC	1 cut(s)	154
BclI	TGATCA	1 cut(s)	1384
BcnI	CCSGG	3 cut(s)	142, 1188, 1364
BcoDI	GTCTC	2 cut(s)	39, 1281
BfaI	CTAG	5 cut(s)	840, 1080, 1176, 1214, 2070
BfmI	CTRYAG	2 cut(s)	653, 876
BfoI	RGCGCY	3 cut(s)	1214, 1864, 2136
BfuAI	ACCTGC	2 cut(s)	1867, 2015
BfuI	GTATCC	1 cut(s)	154
BglII	AGATCT	1 cut(s)	259
BisI	GCNGC	6 cut(s)	656, 810, 1472, 1926, 1944, 2079
BlsI	GCNGC	6 cut(s)	657, 811, 1473, 1927, 1945, 2080
BmcAI	AGTACT	1 cut(s)	1115
Bme1390I	CCNGG	5 cut(s)	142, 1163, 1188, 1364, 1735
Bme18I	GGWCC	1 cut(s)	987
BmeT110I	CYCGRG	2 cut(s)	525, 2136
BmgBI	CACGTC	1 cut(s)	21
BmgT120I	GGNCC	2 cut(s)	801, 987
BmiI	GGNNCC	7 cut(s)	363, 1475, 1503, 1746, 1862, 2029, 2053
BmrFI	CCNGG	5 cut(s)	142, 1163, 1188, 1364, 1735
BmsI	GCATC	5 cut(s)	407, 454, 1323, 1528, 2179
BmtI	GCTAGC	1 cut(s)	1217
BoxI	GACNNNNGTC	1 cut(s)	205
BplI	GAGNNNNNCTC	2 cut(s)	1489, 1521
BpmI	CTGGAG	4 cut(s)	476, 684, 1256, 2148
BpuEI	CTTGAG	3 cut(s)	188, 1313, 1664
BpuMI	CCSGG	3 cut(s)	142, 1188, 1364
BsaAI	YACGTR	2 cut(s)	1409, 1634
BsaBI	GATNNNNATC	1 cut(s)	963
BsaHI	GRCGYC	3 cut(s)	1639, 1861, 1951
BsaJI	CCNNGG	2 cut(s)	1162, 1734
BsaXI	ACNNNNNCTCC	4 cut(s)	1344, 1374, 1593, 1623
Bsc4I	CCNNNNNNNGG	3 cut(s)	427, 1483, 1754
Bse118I	RCCGGY	1 cut(s)	803
Bse21I	CCTNAGG	1 cut(s)	294
Bse3DI	GCAATG	2 cut(s)	510, 892
Bse8I	GATNNNNATC	1 cut(s)	963
BseBI	CCWGG	2 cut(s)	1163, 1735
BseDI	CCNNGG	2 cut(s)	1162, 1734
BseGI	GGATG	6 cut(s)	70, 964, 1338, 1423, 1528, 1935
BseJI	GATNNNNATC	1 cut(s)	963
BseLI	CCNNNNNNNGG	3 cut(s)	427, 1483, 1754
BseMI	GCAATG	2 cut(s)	510, 892
BseMII	CTCAG	4 cut(s)	308, 969, 1468, 2141
BseRI	GAGGAG	4 cut(s)	1310, 1436, 1510, 1736
BseSI	GKGCMC	2 cut(s)	1741, 2032
BseXI	GCAGC	4 cut(s)	667, 796, 1912, 2065
BseYI	CCCAGC	1 cut(s)	559
BsgI	GTGCAG	1 cut(s)	264
Bsh1236I	CGCG	1 cut(s)	2094
BshFI	GGCC	2 cut(s)	803, 1161
BshNI	GGYRCC	4 cut(s)	1473, 1860, 2027, 2051
BsiHKAI	GWGCWC	1 cut(s)	151
BsiHKCI	CYCGRG	2 cut(s)	525, 2136
BsiSI	CCGG	5 cut(s)	142, 804, 1188, 1363, 1987
BslI	CCNNNNNNNGG	3 cut(s)	427, 1483, 1754
BsmAI	GTCTC	2 cut(s)	39, 1281
BsmI	GAATGC	1 cut(s)	2119
BsnI	GGCC	2 cut(s)	803, 1161
BsoBI	CYCGRG	2 cut(s)	525, 2136
Bsp1286I	GDGCHC	5 cut(s)	151, 1170, 1741, 2032, 2172
Bsp143I	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
BspACI	CCGC	7 cut(s)	350, 956, 1471, 1687, 1943, 2032, 2094
BspANI	GGCC	2 cut(s)	803, 1161
BspCNI	CTCAG	4 cut(s)	307, 970, 1467, 2140
BspFNI	CGCG	1 cut(s)	2094
BspLI	GGNNCC	7 cut(s)	363, 1475, 1503, 1746, 1862, 2029, 2053
BspMAI	CTGCAG	1 cut(s)	657
BspMI	ACCTGC	2 cut(s)	1867, 2015
BspOI	GCTAGC	1 cut(s)	1217
BspPI	GGATC	7 cut(s)	356, 369, 1225, 1458, 1598, 1739, 1752
BspQI	GCTCTTC	1 cut(s)	1824
BspT107I	GGYRCC	4 cut(s)	1473, 1860, 2027, 2051
BsrDI	GCAATG	2 cut(s)	510, 892
BsrFI	RCCGGY	1 cut(s)	803
BssAI	RCCGGY	1 cut(s)	803
BssECI	CCNNGG	2 cut(s)	1162, 1734
BssMI	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
BssNI	GRCGYC	3 cut(s)	1639, 1861, 1951
BssSI	CACGAG	1 cut(s)	240
Bst2BI	CACGAG	1 cut(s)	240
Bst2UI	CCWGG	2 cut(s)	1163, 1735
Bst4CI	ACNGT	9 cut(s)	11, 27, 32, 407, 953, 1118, 1124, 1786, 1911
Bst6I	CTCTTC	2 cut(s)	1824, 1989
BstACI	GRCGYC	3 cut(s)	1639, 1861, 1951
BstAPI	GCANNNNNTGC	3 cut(s)	930, 1333, 2123
BstBAI	YACGTR	2 cut(s)	1409, 1634
BstC8I	GCNNGC	9 cut(s)	681, 837, 899, 931, 1215, 1550, 1874, 1930, 2032
BstDEI	CTNAG	6 cut(s)	294, 978, 1095, 1110, 1454, 2127
BstF5I	GGATG	6 cut(s)	70, 964, 1338, 1423, 1528, 1935
BstFNI	CGCG	1 cut(s)	2094
BstH2I	RGCGCY	3 cut(s)	1214, 1864, 2136
BstHHI	GCGC	3 cut(s)	1213, 1863, 2135
BstKTI	GATC	8 cut(s)	221, 262, 364, 1233, 1387, 1453, 1606, 1747
BstMAI	GTCTC	2 cut(s)	39, 1281
BstMBI	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
BstNI	CCWGG	2 cut(s)	1163, 1735
BstNSI	RCATGY	2 cut(s)	897, 933
BstPAI	GACNNNNGTC	1 cut(s)	205
BstSCI	CCNGG	5 cut(s)	140, 1161, 1186, 1362, 1733
BstSFI	CTRYAG	2 cut(s)	653, 876
BstSLI	GKGCMC	2 cut(s)	1741, 2032
BstUI	CGCG	1 cut(s)	2094
BstV1I	GCAGC	4 cut(s)	667, 796, 1912, 2065
BstX2I	RGATCY	5 cut(s)	259, 361, 1450, 1603, 1744
BstYI	RGATCY	5 cut(s)	259, 361, 1450, 1603, 1744
Bsu36I	CCTNAGG	1 cut(s)	294
BsuI	GTATCC	1 cut(s)	154
BsuRI	GGCC	2 cut(s)	803, 1161
BtgZI	GCGATG	1 cut(s)	1325
BtrI	CACGTC	1 cut(s)	21
BtsCI	GGATG	6 cut(s)	70, 964, 1338, 1423, 1528, 1935
BtsI	GCAGTG	3 cut(s)	252, 931, 1334
BtsIMutI	CAGTG	7 cut(s)	252, 931, 1003, 1334, 1739, 1916, 2018
BveI	ACCTGC	2 cut(s)	1867, 2015
Cac8I	GCNNGC	9 cut(s)	681, 837, 899, 931, 1215, 1550, 1874, 1930, 2032
CfoI	GCGC	3 cut(s)	1213, 1863, 2135
Cfr10I	RCCGGY	1 cut(s)	803
Cfr13I	GGNCC	2 cut(s)	801, 987
CseI	GACGC	2 cut(s)	1647, 1940
CspCI	CAANNNNNGTGG	2 cut(s)	979, 1014
CviAII	CATG	7 cut(s)	339, 484, 894, 930, 1920, 1947, 1975
DdeI	CTNAG	6 cut(s)	294, 978, 1095, 1110, 1454, 2127
DinI	GGCGCC	1 cut(s)	1862
DpnI	GATC	8 cut(s)	220, 261, 363, 1232, 1386, 1452, 1605, 1746
DpnII	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
DraI	TTTAAA	1 cut(s)	172
EaeI	YGGCCR	1 cut(s)	1159
Eam1104I	CTCTTC	2 cut(s)	1824, 1989
EarI	CTCTTC	2 cut(s)	1824, 1989
Eco24I	GRGCYC	2 cut(s)	1170, 2172
Eco32I	GATATC	1 cut(s)	1465
Eco47I	GGWCC	1 cut(s)	987
Eco47III	AGCGCT	2 cut(s)	1212, 2134
Eco57I	CTGAAG	4 cut(s)	216, 276, 701, 1848
Eco72I	CACGTG	1 cut(s)	1409
Eco81I	CCTNAGG	1 cut(s)	294
Eco88I	CYCGRG	2 cut(s)	525, 2136
EcoRII	CCWGG	2 cut(s)	1161, 1733
EcoRV	GATATC	1 cut(s)	1465
EcoT22I	ATGCAT	1 cut(s)	400
EcoT38I	GRGCYC	2 cut(s)	1170, 2172
EgeI	GGCGCC	1 cut(s)	1862
EheI	GGCGCC	1 cut(s)	1862
FaeI	CATG	7 cut(s)	342, 487, 897, 933, 1923, 1950, 1978
FalI	AAGNNNNNCTT	2 cut(s)	566, 598
FatI	CATG	7 cut(s)	338, 483, 893, 929, 1919, 1946, 1974
FauI	CCCGC	1 cut(s)	2039
FbaI	TGATCA	1 cut(s)	1384
FblI	GTMKAC	1 cut(s)	1441
Fnu4HI	GCNGC	6 cut(s)	656, 810, 1472, 1926, 1944, 2079
FokI	GGATG	6 cut(s)	77, 971, 1345, 1430, 1535, 1922
FriOI	GRGCYC	2 cut(s)	1170, 2172
Fsp4HI	GCNGC	6 cut(s)	656, 810, 1472, 1926, 1944, 2079
FspBI	CTAG	5 cut(s)	840, 1080, 1176, 1214, 2070
GlaI	GCGC	3 cut(s)	1212, 1862, 2134
GluI	GCNGC	6 cut(s)	656, 810, 1472, 1926, 1944, 2079
GsaI	CCCAGC	1 cut(s)	563
GsuI	CTGGAG	4 cut(s)	476, 684, 1256, 2148
HaeII	RGCGCY	3 cut(s)	1214, 1864, 2136
HaeIII	GGCC	2 cut(s)	803, 1161
HapII	CCGG	5 cut(s)	142, 804, 1188, 1363, 1987
HgaI	GACGC	2 cut(s)	1647, 1940
HhaI	GCGC	3 cut(s)	1213, 1863, 2135
Hin1I	GRCGYC	3 cut(s)	1639, 1861, 1951
Hin1II	CATG	7 cut(s)	342, 487, 897, 933, 1923, 1950, 1978
Hin6I	GCGC	3 cut(s)	1211, 1861, 2133
HinP1I	GCGC	3 cut(s)	1211, 1861, 2133
HincII	GTYRAC	1 cut(s)	1612
HindII	GTYRAC	1 cut(s)	1612
HinfI	GANTC	7 cut(s)	74, 201, 741, 964, 1056, 1319, 1438
HpaII	CCGG	5 cut(s)	142, 804, 1188, 1363, 1987
HphI	GGTGA	2 cut(s)	554, 562
Hpy166II	GTNNAC	7 cut(s)	119, 545, 1154, 1442, 1572, 1612, 1637
Hpy188III	TCNNGA	8 cut(s)	78, 296, 663, 1080, 1456, 1595, 2138, 2165
Hpy8I	GTNNAC	7 cut(s)	119, 545, 1154, 1442, 1572, 1612, 1637
Hpy99I	CGWCG	1 cut(s)	1956
HpyCH4III	ACNGT	9 cut(s)	11, 27, 32, 407, 953, 1118, 1124, 1786, 1911
HpyCH4IV	ACGT	4 cut(s)	20, 164, 1408, 1633
HpyF3I	CTNAG	6 cut(s)	294, 978, 1095, 1110, 1454, 2127
HpySE526I	ACGT	4 cut(s)	20, 164, 1408, 1633
Hsp92I	GRCGYC	3 cut(s)	1639, 1861, 1951
Hsp92II	CATG	7 cut(s)	342, 487, 897, 933, 1923, 1950, 1978
HspAI	GCGC	3 cut(s)	1211, 1861, 2133
KasI	GGCGCC	1 cut(s)	1860
Ksp22I	TGATCA	1 cut(s)	1384
Kzo9I	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
LguI	GCTCTTC	1 cut(s)	1824
LmnI	GCTCC	5 cut(s)	154, 520, 1366, 1723, 1978
Lsp1109I	GCAGC	4 cut(s)	667, 796, 1912, 2065
LweI	GCATC	5 cut(s)	407, 454, 1323, 1528, 2179
MaeI	CTAG	5 cut(s)	840, 1080, 1176, 1214, 2070
MaeII	ACGT	4 cut(s)	20, 164, 1408, 1633
MaeIII	GTNAC	4 cut(s)	21, 1118, 1137, 1651
MalI	GATC	8 cut(s)	220, 261, 363, 1232, 1386, 1452, 1605, 1746
MboI	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
MflI	RGATCY	5 cut(s)	259, 361, 1450, 1603, 1744
MhlI	GDGCHC	5 cut(s)	151, 1170, 1741, 2032, 2172
MlsI	TGGCCA	1 cut(s)	1161
MluCI	AATT	7 cut(s)	302, 1015, 1083, 1144, 1426, 1838, 2150
MluNI	TGGCCA	1 cut(s)	1161
Mly113I	GGCGCC	1 cut(s)	1861
MlyI	GAGTC	4 cut(s)	68, 195, 750, 1447
MmeI	TCCRAC	3 cut(s)	176, 1014, 1380
Mox20I	TGGCCA	1 cut(s)	1161
Mph1103I	ATGCAT	1 cut(s)	400
MroXI	GAANNNNTTC	1 cut(s)	1087
MscI	TGGCCA	1 cut(s)	1161
MseI	TTAA	6 cut(s)	123, 171, 264, 752, 1148, 1308
Msp20I	TGGCCA	1 cut(s)	1161
MspA1I	CMGCKG	2 cut(s)	776, 977
MspI	CCGG	5 cut(s)	142, 804, 1188, 1363, 1987
MspR9I	CCNGG	5 cut(s)	142, 1163, 1188, 1364, 1735
Mva1269I	GAATGC	1 cut(s)	2119
MvaI	CCWGG	2 cut(s)	1163, 1735
MvnI	CGCG	1 cut(s)	2094
NarI	GGCGCC	1 cut(s)	1861
NciI	CCSGG	3 cut(s)	142, 1188, 1364
NdeII	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
NheI	GCTAGC	1 cut(s)	1213
NlaIII	CATG	7 cut(s)	342, 487, 897, 933, 1923, 1950, 1978
NlaIV	GGNNCC	7 cut(s)	363, 1475, 1503, 1746, 1862, 2029, 2053
NmuCI	GTSAC	2 cut(s)	21, 1651
NsiI	ATGCAT	1 cut(s)	400
NspI	RCATGY	2 cut(s)	897, 933
PaeI	GCATGC	1 cut(s)	933
PaeR7I	CTCGAG	1 cut(s)	2136
PaqCI	CACCTGC	1 cut(s)	2015
PciSI	GCTCTTC	1 cut(s)	1824
PctI	GAATGC	1 cut(s)	2119
PdmI	GAANNNNTTC	1 cut(s)	1087
PfeI	GAWTC	3 cut(s)	964, 1056, 1319
PfoI	TCCNGGA	2 cut(s)	1186, 1362
PkrI	GCNGC	6 cut(s)	657, 811, 1473, 1927, 1945, 2080
PleI	GAGTC	4 cut(s)	68, 195, 749, 1446
PluTI	GGCGCC	1 cut(s)	1864
PmaCI	CACGTG	1 cut(s)	1409
PmlI	CACGTG	1 cut(s)	1409
PpsI	GAGTC	4 cut(s)	68, 195, 749, 1446
Ppu21I	YACGTR	2 cut(s)	1409, 1634
PshAI	GACNNNNGTC	1 cut(s)	205
Psp6I	CCWGG	2 cut(s)	1161, 1733
PspCI	CACGTG	1 cut(s)	1409
PspFI	CCCAGC	1 cut(s)	559
PspGI	CCWGG	2 cut(s)	1161, 1733
PspN4I	GGNNCC	7 cut(s)	363, 1475, 1503, 1746, 1862, 2029, 2053
PspPI	GGNCC	2 cut(s)	801, 987
PsrI	GAACNNNNNNTAC	2 cut(s)	763, 795
PstI	CTGCAG	1 cut(s)	657
PsuI	RGATCY	5 cut(s)	259, 361, 1450, 1603, 1744
PvuII	CAGCTG	2 cut(s)	776, 977
SapI	GCTCTTC	1 cut(s)	1824
SaqAI	TTAA	6 cut(s)	123, 171, 264, 752, 1148, 1308
SatI	GCNGC	6 cut(s)	656, 810, 1472, 1926, 1944, 2079
Sau3AI	GATC	8 cut(s)	218, 259, 361, 1230, 1384, 1450, 1603, 1744
Sau96I	GGNCC	2 cut(s)	801, 987
ScaI	AGTACT	1 cut(s)	1115
SchI	GAGTC	4 cut(s)	68, 195, 750, 1447
ScrFI	CCNGG	5 cut(s)	142, 1163, 1188, 1364, 1735
SduI	GDGCHC	5 cut(s)	151, 1170, 1741, 2032, 2172
SfaNI	GCATC	5 cut(s)	407, 454, 1323, 1528, 2179
SfcI	CTRYAG	2 cut(s)	653, 876
SfoI	GGCGCC	1 cut(s)	1862
Sfr274I	CTCGAG	1 cut(s)	2136
SinI	GGWCC	1 cut(s)	987
SlaI	CTCGAG	1 cut(s)	2136
SmlI	CTYRAG	4 cut(s)	203, 1292, 1643, 2136
SmoI	CTYRAG	4 cut(s)	203, 1292, 1643, 2136
SphI	GCATGC	1 cut(s)	933
Sse9I	AATT	7 cut(s)	302, 1015, 1083, 1144, 1426, 1838, 2150
SsiI	CCGC	7 cut(s)	350, 956, 1471, 1687, 1943, 2032, 2094
SspDI	GGCGCC	1 cut(s)	1860
SspI	AATATT	1 cut(s)	127
SspMI	CTAG	5 cut(s)	840, 1080, 1176, 1214, 2070
StyD4I	CCNGG	5 cut(s)	140, 1161, 1186, 1362, 1733
TaaI	ACNGT	9 cut(s)	11, 27, 32, 407, 953, 1118, 1124, 1786, 1911
TaiI	ACGT	4 cut(s)	23, 167, 1411, 1636
TaqI	TCGA	8 cut(s)	79, 217, 739, 1288, 1594, 1806, 2104, 2137
TaqII	GACCGA	1 cut(s)	435
TasI	AATT	7 cut(s)	302, 1015, 1083, 1144, 1426, 1838, 2150
TatI	WGTACW	6 cut(s)	616, 720, 828, 911, 1113, 1151
TauI	GCSGC	2 cut(s)	1474, 1946
TfiI	GAWTC	3 cut(s)	964, 1056, 1319
Tru1I	TTAA	6 cut(s)	123, 171, 264, 752, 1148, 1308
Tru9I	TTAA	6 cut(s)	123, 171, 264, 752, 1148, 1308
TscAI	CASTG	7 cut(s)	252, 931, 1003, 1334, 1746, 1916, 2025
TseFI	GTSAC	2 cut(s)	21, 1651
TseI	GCWGC	4 cut(s)	655, 809, 1925, 2078
Tsp45I	GTSAC	2 cut(s)	21, 1651
TspDTI	ATGAA	9 cut(s)	586, 694, 1080, 1093, 1615, 1761, 1856, 1924, 2090
TspGWI	ACGGA	1 cut(s)	1883
TspRI	CASTG	7 cut(s)	252, 931, 1003, 1334, 1746, 1916, 2025
VpaK11BI	GGWCC	1 cut(s)	987
XapI	RAATTY	4 cut(s)	302, 1144, 1426, 1838
XbaI	TCTAGA	1 cut(s)	1079
XceI	RCATGY	2 cut(s)	897, 933
XhoI	CTCGAG	1 cut(s)	2136
XmiI	GTMKAC	1 cut(s)	1441
XmnI	GAANNNNTTC	1 cut(s)	1087
XspI	CTAG	5 cut(s)	840, 1080, 1176, 1214, 2070
ZrmI	AGTACT	1 cut(s)	1115
Zsp2I	ATGCAT	1 cut(s)	400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.