Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002170

Physical Location & Seq

Genomic Coordinates Forward (+)

466437 .. 470974

Gene Structure 4538 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1007200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2187 bp

ATGTTGGATTGTGGTTACAACAAGCCACAGTACTTGGGAGGCCAGAGAGACAAGTTTGTGAGGTTAGATGACTTGGACTCTAGATTATCATCACCTTCTGATTCAGGAGGTAGAAGGTGTGGGTTTAATATTGAACTAACAAGGACTGCTCCTGCGAGGGATACATCTTTTAAGAGAGGAATGAAAAAGGGGTCTGAAGGACTTAAGTCAATTGGCCGATCACTTGGATTTGGGATCTCTCGTGCAGTATTCCCTGAAGATCTTAAGGGGTCAGACAAGAAGATATATGATCCTCAGGACAAATTTCTCCTGCTGTGGAATAAATTTTTTGTCCTCTCATGTATTCTGGCTGTATCTGTGGACCCTCTGTTTTTCTATCTTCCAGTCATCAATAGTTCATCAACATGTCTTGGCATAGATCGAAGGTTGGCTATCATAGCGACCACATTGCGGATGATTGTTGATGCTTTCTATCTTATTCACATGGCTCTCCAGTTCAGGACAGCTTACATTGCTCCATCATCTCGGGTTTTTGGAAGAGGTGAACTGGTGATTGATCCAGCACGAATAGCGAAGCGATACTTACGAAGTTATTTCATCATTGATTTTCTTTCAGTGCTACCCCTACCACAGATTGTTGTTTGGAGGTTTCTTCAGAGGTCCAAAAGTTCAGATGTGCTTGCTACAAAACAGGCTTTGCTTTTCATAGTTTTATTTCAGTATATTCCCAGACTTCTTCGAGTCCATCCTTTGACTTCAGAGCTGAAAAGAACAGCTGGCGTCCTTGCTGAAACTGCTTGGGCAGGCGCTGCATACTATTTGCTATTATATATGCTCGCTAGTCATATAGTTGGGGCATTTTGGTATTTGTTAGCCTTAGAACGCAATGACACATGCTGGCAGAAGGCTTGTACTGAAATTGGAAAATGTGATAAAAAGATCTTGTACTGCGGGAACCAATACATTTTAGATCCTGCAACTTGGTCCAATGTTACCAAAACCATCTTATCAAAATGCACGGCAGATGACCAGAATACATTCTTTGATTTTGGAATCTACAATTCGGCTTTATCATCTGGGGTTGTTTCATCCAATAAGTTCATCGTCAAATACTGTTACTGTTTGTGGTGGGGACTACAGAATTTAAGTACACTTGGCCAGGGTCTTGCAACCAGCACCTATCCTGGAGAGGTTATATTTTCCATAGCACTGGCTATATTTGGACTGATCCTCTTTGCACTTTTGATAGGAAACATGCAGACCTATCTTCAATCTCTTACTATTCGACTTGAGGAGATGAGAGTCAAAAGACGCGATTCAGAGCAGTGGATGCATCACCGCTTGCTCCCACAAGACCTTAGGGAACGCGTCAGGCGATATGATCAATATAAGTGGTTGGAAACACGTGGTGTGGACGAGCAGAGTTTAGTTCAGAGTCTTCCAAAGGATCTCAGGAGAGATATCAAGCGGCACCTCTGTTTGGCATTAGTGAGGAGGGTTCCACTGTTTGAGGATATGGACGAGAGGTTGCTTGATGCCATTTGTGAGAGACTGAAACCAAGTTTATTCACAGAGAGTACTTACATAGTTCGGGAAGGAGATCCCGTTGACGAGATGCTTTTCATCATACGTGGTCGCCTGGAGAGCATAACAACAGATGGTGGTAGGAGTGGGTTTTTTAACCGCAGTTTACTGAAGGAAGGCGATTTCTGTGGTGAGGAGCTTCTAACTTGGGCACTGGATCCCAAATCTGGTGCTAACCTCCCATCATCTACTCGGACGGTAAAGGCTTTAACAGAGGTTGAGGCTTTTGCCCTTGTAGCCGAAGAGTTGAAATTTGTTGCCAGTCAGTTTAGGCGCCTTCACAGTAGACAGGTTCAGCACACCTTCCGTTTCTACTCACAACAGTGGCGGACCTGGGCTGCTTGCTTTATCCAAGCAGCATGGCGCCGCTATTCCAAGAGGAAAATCTTGGATCTTCGTCGTAAGGAAGAGGAAGCAGAAGCAGAAGCCTTGGGAGGTGCCCGCAGCAATGCTGGAGGTTCGTATAGTATTGGTGCCACGTTTTTAGCTTCTAGGTTTGCTGCAAACGCACTTCGTGGTGTTCACCGGAACAGGAATTTGAAGAGTGCTCGAGAATTAGTCAAATTACAAAAGCCTCCGGAGCCCGATTTTAGCGCTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005217 GO:0005221 GO:0005223 GO:0005244 GO:0005249 GO:0005261 GO:0005262 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006816 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015077 GO:0015079 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042391 GO:0043855 GO:0044425 GO:0044459 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071804 GO:0071805 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

729

Amino Acids

83.1

Weight (kDa)

9.44

Isoelectric Point (pI)

53.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	105 - 428	1.4e-23	Ion transport protein
cNMP_binding	PF00027	523 - 612	7.6e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002170)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT4G30560 AT4G30560 AT4G30560 AT4G30560 AT4G30560 AT5G57940 AT5G57940 AT5G57940
fragaria_vesca	FvH4_1g00870 FvH4_1g00870 FvH4_1g00870 FvH4_1g00870
malus_domestica	MD02G1007200.v1.1 MD15G1144500.v1.1
prunus_persica	Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1
pyrus_communis	pycom02g00610 pycom02g00620 pycom15g13600 pycom15g13610
rosa_chinensis	RchiOBHm_Chr2g0085581
rosa_laevigata	RLG00000015703
rosa_multiflora	Rmu_co8217668.1_g000001 Rmu_ssc0000347.1_g000007
rosa_roxburghii	Rroxscaffold_2G00155250
rosa_rugosa	Rorug01G0461400
rosa_samantha	Rh2AG010500 Rh2BG011100 Rh2CG012000 Rh2DG013000
rosa_wichuraiana	Rw2G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	5 cut(s)	1470, 1857, 1947, 2021, 2057
AccI	GTMKAC	1 cut(s)	1870
AccII	CGCG	2 cut(s)	1314, 1368
AccIII	TCCGGA	1 cut(s)	2161
AciI	CCGC	8 cut(s)	451, 951, 1339, 1468, 1684, 1912, 1951, 2026
AcoI	YGGCCR	2 cut(s)	214, 1156
AcsI	RAATTY	5 cut(s)	302, 323, 1141, 1835, 2119
AcuI	CTGAAG	5 cut(s)	216, 276, 638, 741, 1715
AcvI	CACGTG	1 cut(s)	1406
AcyI	GRCGYC	3 cut(s)	780, 1858, 1948
AdeI	CACNNNGTG	2 cut(s)	1409, 2099
AfaI	GTAC	5 cut(s)	32, 913, 947, 1150, 1579
AfeI	AGCGCT	1 cut(s)	2179
AfiI	CCNNNNNNNGG	3 cut(s)	450, 1480, 1751
AflII	CTTAAG	2 cut(s)	203, 263
AflIII	ACRYGT	3 cut(s)	404, 1366, 1403
AgsI	TTSAA	4 cut(s)	134, 1271, 1834, 2125
AjnI	CCWGG	4 cut(s)	1158, 1183, 1638, 1916
AleI	CACNNNNGTG	1 cut(s)	1906
AluBI	AGCT	5 cut(s)	506, 763, 776, 1723, 2072
AluI	AGCT	5 cut(s)	506, 763, 776, 1723, 2072
Alw21I	GWGCWC	1 cut(s)	2134
Alw26I	GTCTC	2 cut(s)	42, 1543
AlwNI	CAGNNNCTG	1 cut(s)	809
Ama87I	CYCGRG	2 cut(s)	525, 2133
Aor13HI	TCCGGA	1 cut(s)	2161
Aor51HI	AGCGCT	1 cut(s)	2179
AoxI	GGCC	3 cut(s)	40, 214, 1156
ApeKI	GCWGC	5 cut(s)	809, 1922, 1940, 2028, 2084
ApoI	RAATTY	5 cut(s)	302, 323, 1141, 1835, 2119
Asp700I	GAANNNNTTC	1 cut(s)	1037
AspLEI	GCGC	4 cut(s)	809, 1860, 1950, 2180
AspS9I	GGNCC	4 cut(s)	361, 660, 984, 1914
AsuHPI	GGTGA	6 cut(s)	84, 554, 562, 1328, 1727, 2099
AvaI	CYCGRG	2 cut(s)	525, 2133
AvaII	GGWCC	4 cut(s)	361, 660, 984, 1914
AxyI	CCTNAGG	2 cut(s)	294, 1358
BaeGI	GKGCMC	2 cut(s)	1738, 2026
BalI	TGGCCA	1 cut(s)	1158
BamHI	GGATCC	1 cut(s)	1741
BanI	GGYRCC	5 cut(s)	1470, 1857, 1947, 2021, 2057
BanII	GRGCYC	1 cut(s)	2169
BauI	CACGAG	1 cut(s)	240
BbrPI	CACGTG	1 cut(s)	1406
BbsI	GAAGAC	1 cut(s)	1430
Bbv12I	GWGCWC	1 cut(s)	2134
BbvI	GCAGC	5 cut(s)	796, 1909, 1952, 2040, 2071
BccI	CCATC	5 cut(s)	526, 753, 1010, 1652, 1774
BceAI	ACGGC	1 cut(s)	1035
BcgI	CGANNNNNNTGC	2 cut(s)	430, 464
BciT130I	CCWGG	4 cut(s)	1160, 1185, 1640, 1918
BciVI	GTATCC	1 cut(s)	154
BclI	TGATCA	1 cut(s)	1381
BcoDI	GTCTC	2 cut(s)	42, 1543
BfaI	CTAG	3 cut(s)	81, 840, 2076
BfmI	CTRYAG	1 cut(s)	1136
BfoI	RGCGCY	4 cut(s)	810, 1861, 1951, 2181
BfrI	CTTAAG	2 cut(s)	203, 263
BfuI	GTATCC	1 cut(s)	154
BglII	AGATCT	2 cut(s)	259, 939
BisI	GCNGC	7 cut(s)	810, 1469, 1923, 1941, 1951, 2029, 2085
BlsI	GCNGC	7 cut(s)	811, 1470, 1924, 1942, 1952, 2030, 2086
BmcAI	AGTACT	2 cut(s)	32, 1579
Bme1390I	CCNGG	4 cut(s)	1160, 1185, 1640, 1918
Bme18I	GGWCC	4 cut(s)	361, 660, 984, 1914
BmeT110I	CYCGRG	2 cut(s)	525, 2133
BmgT120I	GGNCC	4 cut(s)	361, 660, 984, 1914
BmrFI	CCNGG	4 cut(s)	1160, 1185, 1640, 1918
BmsI	GCATC	5 cut(s)	454, 1320, 1342, 1525, 1605
BoxI	GACNNNNGTC	1 cut(s)	205
BpiI	GAAGAC	1 cut(s)	1430
BpmI	CTGGAG	4 cut(s)	476, 1206, 1661, 2058
BpuEI	CTTGAG	1 cut(s)	1310
BsaAI	YACGTR	2 cut(s)	1406, 1631
BsaBI	GATNNNNATC	1 cut(s)	88
BsaHI	GRCGYC	3 cut(s)	780, 1858, 1948
BsaJI	CCNNGG	3 cut(s)	1159, 1917, 2013
BsaWI	WCCGGW	2 cut(s)	2109, 2161
BsaXI	ACNNNNNCTCC	2 cut(s)	1590, 1620
Bsc4I	CCNNNNNNNGG	3 cut(s)	450, 1480, 1751
Bse1I	ACTGG	6 cut(s)	383, 493, 552, 1215, 1743, 1845
Bse21I	CCTNAGG	2 cut(s)	294, 1358
Bse3DI	GCAATG	4 cut(s)	446, 510, 892, 2038
Bse8I	GATNNNNATC	1 cut(s)	88
BseAI	TCCGGA	1 cut(s)	2161
BseBI	CCWGG	4 cut(s)	1160, 1185, 1640, 1918
BseDI	CCNNGG	3 cut(s)	1159, 1917, 2013
BseGI	GGATG	4 cut(s)	459, 745, 1088, 1335
BseJI	GATNNNNATC	1 cut(s)	88
BseLI	CCNNNNNNNGG	3 cut(s)	450, 1480, 1751
BseMI	GCAATG	4 cut(s)	446, 510, 892, 2038
BseMII	CTCAG	2 cut(s)	308, 1465
BseNI	ACTGG	6 cut(s)	383, 493, 552, 1215, 1743, 1845
BseRI	GAGGAG	3 cut(s)	1307, 1507, 1733
BseSI	GKGCMC	2 cut(s)	1738, 2026
BseXI	GCAGC	5 cut(s)	796, 1909, 1952, 2040, 2071
BsgI	GTGCAG	1 cut(s)	264
Bsh1236I	CGCG	2 cut(s)	1314, 1368
BshFI	GGCC	3 cut(s)	42, 216, 1158
BshNI	GGYRCC	5 cut(s)	1470, 1857, 1947, 2021, 2057
BsiHKAI	GWGCWC	1 cut(s)	2134
BsiHKCI	CYCGRG	2 cut(s)	525, 2133
BsiSI	CCGG	2 cut(s)	2110, 2162
BslFI	GGGAC	1 cut(s)	1146
BslI	CCNNNNNNNGG	3 cut(s)	450, 1480, 1751
BsmAI	GTCTC	2 cut(s)	42, 1543
BsmFI	GGGAC	1 cut(s)	1146
BsnI	GGCC	3 cut(s)	42, 216, 1158
BsoBI	CYCGRG	2 cut(s)	525, 2133
Bsp1286I	GDGCHC	4 cut(s)	1738, 2026, 2134, 2169
Bsp13I	TCCGGA	1 cut(s)	2161
BspACI	CCGC	8 cut(s)	451, 951, 1339, 1468, 1684, 1912, 1951, 2026
BspANI	GGCC	3 cut(s)	42, 216, 1158
BspCNI	CTCAG	2 cut(s)	307, 1464
BspEI	TCCGGA	1 cut(s)	2161
BspFNI	CGCG	2 cut(s)	1314, 1368
BspT107I	GGYRCC	5 cut(s)	1470, 1857, 1947, 2021, 2057
BspTI	CTTAAG	2 cut(s)	203, 263
BsrDI	GCAATG	4 cut(s)	446, 510, 892, 2038
BsrI	ACTGG	6 cut(s)	383, 493, 552, 1215, 1743, 1845
BssECI	CCNNGG	3 cut(s)	1159, 1917, 2013
BssNI	GRCGYC	3 cut(s)	780, 1858, 1948
BssSI	CACGAG	1 cut(s)	240
BssT1I	CCWWGG	1 cut(s)	2013
Bst2BI	CACGAG	1 cut(s)	240
Bst2UI	CCWGG	4 cut(s)	1160, 1185, 1640, 1918
Bst4CI	ACNGT	7 cut(s)	30, 1115, 1121, 1506, 1783, 1868, 1908
Bst6I	CTCTTC	4 cut(s)	532, 1821, 1986, 2120
BstACI	GRCGYC	3 cut(s)	780, 1858, 1948
BstAFI	CTTAAG	2 cut(s)	203, 263
BstAPI	GCANNNNNTGC	2 cut(s)	809, 1330
BstBAI	YACGTR	2 cut(s)	1406, 1631
BstC8I	GCNNGC	8 cut(s)	681, 778, 805, 837, 899, 1343, 1927, 2026
BstDEI	CTNAG	4 cut(s)	294, 877, 1358, 1451
BstF5I	GGATG	4 cut(s)	459, 745, 1088, 1335
BstFNI	CGCG	2 cut(s)	1314, 1368
BstH2I	RGCGCY	4 cut(s)	810, 1861, 1951, 2181
BstHHI	GCGC	4 cut(s)	809, 1860, 1950, 2180
BstMAI	GTCTC	2 cut(s)	42, 1543
BstNI	CCWGG	4 cut(s)	1160, 1185, 1640, 1918
BstNSI	RCATGY	3 cut(s)	408, 897, 1258
BstPAI	GACNNNNGTC	1 cut(s)	205
BstSCI	CCNGG	4 cut(s)	1158, 1183, 1638, 1916
BstSFI	CTRYAG	1 cut(s)	1136
BstSLI	GKGCMC	2 cut(s)	1738, 2026
BstUI	CGCG	2 cut(s)	1314, 1368
BstV1I	GCAGC	5 cut(s)	796, 1909, 1952, 2040, 2071
BstV2I	GAAGAC	1 cut(s)	1430
BstX2I	RGATCY	8 cut(s)	234, 259, 939, 970, 1447, 1600, 1741, 1975
BstXI	CCANNNNNNTGG	1 cut(s)	1210
BstYI	RGATCY	8 cut(s)	234, 259, 939, 970, 1447, 1600, 1741, 1975
Bsu36I	CCTNAGG	2 cut(s)	294, 1358
BsuI	GTATCC	1 cut(s)	154
BsuRI	GGCC	3 cut(s)	42, 216, 1158
BtsCI	GGATG	4 cut(s)	459, 745, 1088, 1335
BtsI	GCAGTG	1 cut(s)	1331
BtsIMutI	CAGTG	6 cut(s)	621, 1208, 1331, 1502, 1736, 1913
Cac8I	GCNNGC	8 cut(s)	681, 778, 805, 837, 899, 1343, 1927, 2026
CaiI	CAGNNNCTG	1 cut(s)	809
CfoI	GCGC	4 cut(s)	809, 1860, 1950, 2180
Cfr13I	GGNCC	4 cut(s)	361, 660, 984, 1914
CseI	GACGC	3 cut(s)	769, 1320, 1357
Csp6I	GTAC	5 cut(s)	31, 912, 946, 1149, 1578
CspCI	CAANNNNNGTGG	4 cut(s)	15, 50, 1373, 1408
CviAII	CATG	6 cut(s)	339, 405, 484, 894, 1255, 1944
CviQI	GTAC	5 cut(s)	31, 912, 946, 1149, 1578
DdeI	CTNAG	4 cut(s)	294, 877, 1358, 1451
DinI	GGCGCC	2 cut(s)	1859, 1949
DraIII	CACNNNGTG	2 cut(s)	1409, 2099
EaeI	YGGCCR	2 cut(s)	214, 1156
Eam1104I	CTCTTC	4 cut(s)	532, 1821, 1986, 2120
EarI	CTCTTC	4 cut(s)	532, 1821, 1986, 2120
EciI	GGCGGA	1 cut(s)	1927
Eco130I	CCWWGG	1 cut(s)	2013
Eco24I	GRGCYC	1 cut(s)	2169
Eco32I	GATATC	1 cut(s)	1462
Eco47I	GGWCC	4 cut(s)	361, 660, 984, 1914
Eco47III	AGCGCT	1 cut(s)	2179
Eco57I	CTGAAG	5 cut(s)	216, 276, 638, 741, 1715
Eco72I	CACGTG	1 cut(s)	1406
Eco81I	CCTNAGG	2 cut(s)	294, 1358
Eco88I	CYCGRG	2 cut(s)	525, 2133
EcoRII	CCWGG	4 cut(s)	1158, 1183, 1638, 1916
EcoRV	GATATC	1 cut(s)	1462
EcoT14I	CCWWGG	1 cut(s)	2013
EcoT22I	ATGCAT	1 cut(s)	1335
EcoT38I	GRGCYC	1 cut(s)	2169
EgeI	GGCGCC	2 cut(s)	1859, 1949
EheI	GGCGCC	2 cut(s)	1859, 1949
ErhI	CCWWGG	1 cut(s)	2013
FaeI	CATG	6 cut(s)	342, 408, 487, 897, 1258, 1947
FalI	AAGNNNNNCTT	2 cut(s)	566, 598
FaqI	GGGAC	1 cut(s)	1146
FatI	CATG	6 cut(s)	338, 404, 483, 893, 1254, 1943
FauI	CCCGC	2 cut(s)	944, 2033
FbaI	TGATCA	1 cut(s)	1381
FblI	GTMKAC	1 cut(s)	1870
Fnu4HI	GCNGC	7 cut(s)	810, 1469, 1923, 1941, 1951, 2029, 2085
FokI	GGATG	4 cut(s)	466, 732, 1075, 1342
FriOI	GRGCYC	1 cut(s)	2169
Fsp4HI	GCNGC	7 cut(s)	810, 1469, 1923, 1941, 1951, 2029, 2085
FspBI	CTAG	3 cut(s)	81, 840, 2076
GlaI	GCGC	4 cut(s)	808, 1859, 1949, 2179
GluI	GCNGC	7 cut(s)	810, 1469, 1923, 1941, 1951, 2029, 2085
GsuI	CTGGAG	4 cut(s)	476, 1206, 1661, 2058
HaeII	RGCGCY	4 cut(s)	810, 1861, 1951, 2181
HaeIII	GGCC	3 cut(s)	42, 216, 1158
HapII	CCGG	2 cut(s)	2110, 2162
HgaI	GACGC	3 cut(s)	769, 1320, 1357
HhaI	GCGC	4 cut(s)	809, 1860, 1950, 2180
Hin1I	GRCGYC	3 cut(s)	780, 1858, 1948
Hin1II	CATG	6 cut(s)	342, 408, 487, 897, 1258, 1947
Hin6I	GCGC	4 cut(s)	807, 1858, 1948, 2178
HinP1I	GCGC	4 cut(s)	807, 1858, 1948, 2178
HincII	GTYRAC	1 cut(s)	1609
HindII	GTYRAC	1 cut(s)	1609
HinfI	GANTC	7 cut(s)	77, 101, 741, 1053, 1302, 1316, 1435
HpaII	CCGG	2 cut(s)	2110, 2162
HphI	GGTGA	6 cut(s)	84, 554, 562, 1328, 1727, 2099
Hpy166II	GTNNAC	8 cut(s)	361, 545, 1151, 1414, 1609, 1691, 1871, 2107
Hpy188I	TCNGA	9 cut(s)	100, 196, 274, 657, 673, 760, 1321, 1434, 1779
Hpy188III	TCNNGA	8 cut(s)	81, 105, 296, 499, 1453, 1592, 2135, 2162
Hpy8I	GTNNAC	8 cut(s)	361, 545, 1151, 1414, 1609, 1691, 1871, 2107
Hpy99I	CGWCG	1 cut(s)	1986
HpyCH4III	ACNGT	7 cut(s)	30, 1115, 1121, 1506, 1783, 1868, 1908
HpyCH4IV	ACGT	3 cut(s)	1405, 1630, 2063
HpyCH4V	TGCA	9 cut(s)	245, 812, 977, 1017, 1169, 1238, 1258, 1333, 2087
HpyF3I	CTNAG	4 cut(s)	294, 877, 1358, 1451
HpySE526I	ACGT	3 cut(s)	1405, 1630, 2063
Hsp92I	GRCGYC	3 cut(s)	780, 1858, 1948
Hsp92II	CATG	6 cut(s)	342, 408, 487, 897, 1258, 1947
HspAI	GCGC	4 cut(s)	807, 1858, 1948, 2178
KasI	GGCGCC	2 cut(s)	1857, 1947
Kpn2I	TCCGGA	1 cut(s)	2161
Ksp22I	TGATCA	1 cut(s)	1381
LmnI	GCTCC	5 cut(s)	154, 520, 1350, 1720, 2164
Lsp1109I	GCAGC	5 cut(s)	796, 1909, 1952, 2040, 2071
LweI	GCATC	5 cut(s)	454, 1320, 1342, 1525, 1605
MaeI	CTAG	3 cut(s)	81, 840, 2076
MaeII	ACGT	3 cut(s)	1405, 1630, 2063
MaeIII	GTNAC	3 cut(s)	14, 991, 1115
MfeI	CAATTG	1 cut(s)	210
MflI	RGATCY	8 cut(s)	234, 259, 939, 970, 1447, 1600, 1741, 1975
MhlI	GDGCHC	4 cut(s)	1738, 2026, 2134, 2169
MlsI	TGGCCA	1 cut(s)	1158
MluI	ACGCGT	1 cut(s)	1366
MluNI	TGGCCA	1 cut(s)	1158
Mly113I	GGCGCC	2 cut(s)	1858, 1948
MlyI	GAGTC	4 cut(s)	71, 750, 1311, 1444
MmeI	TCCRAC	1 cut(s)	1377
Mox20I	TGGCCA	1 cut(s)	1158
Mph1103I	ATGCAT	1 cut(s)	1335
MroI	TCCGGA	1 cut(s)	2161
MroXI	GAANNNNTTC	1 cut(s)	1037
MscI	TGGCCA	1 cut(s)	1158
MseI	TTAA	8 cut(s)	126, 171, 204, 264, 1145, 1680, 1793, 2185
MslI	CAYNNNNRTG	2 cut(s)	403, 1906
Msp20I	TGGCCA	1 cut(s)	1158
MspA1I	CMGCKG	1 cut(s)	776
MspCI	CTTAAG	2 cut(s)	203, 263
MspI	CCGG	2 cut(s)	2110, 2162
MspR9I	CCNGG	4 cut(s)	1160, 1185, 1640, 1918
MunI	CAATTG	1 cut(s)	210
MvaI	CCWGG	4 cut(s)	1160, 1185, 1640, 1918
MvnI	CGCG	2 cut(s)	1314, 1368
NarI	GGCGCC	2 cut(s)	1858, 1948
NlaIII	CATG	6 cut(s)	342, 408, 487, 897, 1258, 1947
NsiI	ATGCAT	1 cut(s)	1335
NspI	RCATGY	3 cut(s)	408, 897, 1258
OliI	CACNNNNGTG	1 cut(s)	1906
PaeR7I	CTCGAG	1 cut(s)	2133
PciI	ACATGT	1 cut(s)	404
PcsI	WCGNNNNNNNCGW	1 cut(s)	1372
PdmI	GAANNNNTTC	1 cut(s)	1037
PfeI	GAWTC	3 cut(s)	101, 1053, 1316
PfoI	TCCNGGA	1 cut(s)	1183
PkrI	GCNGC	7 cut(s)	811, 1470, 1924, 1942, 1952, 2030, 2086
PleI	GAGTC	4 cut(s)	71, 749, 1310, 1443
PluTI	GGCGCC	2 cut(s)	1861, 1951
PmaCI	CACGTG	1 cut(s)	1406
PmlI	CACGTG	1 cut(s)	1406
PpsI	GAGTC	4 cut(s)	71, 749, 1310, 1443
Ppu21I	YACGTR	2 cut(s)	1406, 1631
PscI	ACATGT	1 cut(s)	404
PshAI	GACNNNNGTC	1 cut(s)	205
Psp6I	CCWGG	4 cut(s)	1158, 1183, 1638, 1916
PspCI	CACGTG	1 cut(s)	1406
PspGI	CCWGG	4 cut(s)	1158, 1183, 1638, 1916
PspPI	GGNCC	4 cut(s)	361, 660, 984, 1914
PstNI	CAGNNNCTG	1 cut(s)	809
PsuI	RGATCY	8 cut(s)	234, 259, 939, 970, 1447, 1600, 1741, 1975
PvuII	CAGCTG	1 cut(s)	776
RsaI	GTAC	5 cut(s)	32, 913, 947, 1150, 1579
RsaNI	GTAC	5 cut(s)	31, 912, 946, 1149, 1578
RseI	CAYNNNNRTG	2 cut(s)	403, 1906
SaqAI	TTAA	8 cut(s)	126, 171, 204, 264, 1145, 1680, 1793, 2185
SatI	GCNGC	7 cut(s)	810, 1469, 1923, 1941, 1951, 2029, 2085
Sau96I	GGNCC	4 cut(s)	361, 660, 984, 1914
ScaI	AGTACT	2 cut(s)	32, 1579
SchI	GAGTC	4 cut(s)	71, 750, 1311, 1444
ScrFI	CCNGG	4 cut(s)	1160, 1185, 1640, 1918
SduI	GDGCHC	4 cut(s)	1738, 2026, 2134, 2169
SfaNI	GCATC	5 cut(s)	454, 1320, 1342, 1525, 1605
SfcI	CTRYAG	1 cut(s)	1136
SfoI	GGCGCC	2 cut(s)	1859, 1949
Sfr274I	CTCGAG	1 cut(s)	2133
SinI	GGWCC	4 cut(s)	361, 660, 984, 1914
SlaI	CTCGAG	1 cut(s)	2133
SmiMI	CAYNNNNRTG	2 cut(s)	403, 1906
SmlI	CTYRAG	4 cut(s)	203, 263, 1289, 2133
SmoI	CTYRAG	4 cut(s)	203, 263, 1289, 2133
SsiI	CCGC	8 cut(s)	451, 951, 1339, 1468, 1684, 1912, 1951, 2026
SspDI	GGCGCC	2 cut(s)	1857, 1947
SspI	AATATT	1 cut(s)	130
SspMI	CTAG	3 cut(s)	81, 840, 2076
StyD4I	CCNGG	4 cut(s)	1158, 1183, 1638, 1916
StyI	CCWWGG	1 cut(s)	2013
TaaI	ACNGT	7 cut(s)	30, 1115, 1121, 1506, 1783, 1868, 1908
TaiI	ACGT	3 cut(s)	1408, 1633, 2066
TaqI	TCGA	4 cut(s)	421, 739, 1285, 2134
TatI	WGTACW	5 cut(s)	30, 911, 945, 1148, 1577
TauI	GCSGC	2 cut(s)	1471, 1953
TfiI	GAWTC	3 cut(s)	101, 1053, 1316
Tru1I	TTAA	8 cut(s)	126, 171, 204, 264, 1145, 1680, 1793, 2185
Tru9I	TTAA	8 cut(s)	126, 171, 204, 264, 1145, 1680, 1793, 2185
TscAI	CASTG	6 cut(s)	621, 1215, 1331, 1509, 1743, 1913
TseI	GCWGC	5 cut(s)	809, 1922, 1940, 2028, 2084
TspDTI	ATGAA	7 cut(s)	197, 387, 586, 694, 1077, 1090, 1612
TspGWI	ACGGA	1 cut(s)	1880
TspRI	CASTG	6 cut(s)	621, 1215, 1331, 1509, 1743, 1913
Vha464I	CTTAAG	2 cut(s)	203, 263
VpaK11BI	GGWCC	4 cut(s)	361, 660, 984, 1914
XapI	RAATTY	5 cut(s)	302, 323, 1141, 1835, 2119
XbaI	TCTAGA	1 cut(s)	80
XceI	RCATGY	3 cut(s)	408, 897, 1258
XhoI	CTCGAG	1 cut(s)	2133
XmiI	GTMKAC	1 cut(s)	1870
XmnI	GAANNNNTTC	1 cut(s)	1037
XspI	CTAG	3 cut(s)	81, 840, 2076
ZrmI	AGTACT	2 cut(s)	32, 1579
Zsp2I	ATGCAT	1 cut(s)	1335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.