Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002170

Physical Location & Seq

Genomic Coordinates Forward (+)

10666179 .. 10675567

Gene Structure 9389 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1144500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGTTGGATTGTGGTTACAACAAGTCACAGTACTTGGGAGGCCAGAGAGACAAGTTTGTGAGGCTGGATGACTTGGACTCTAGATTATCATCGCCTTCTGATTCTGGAGGGAGAAGATGTGGGTTTAATATTGAGGGACTAACCCGGACTGCTCCTGCAAGGGATACATCTTTTAAGAGAGGAATGAAAAAGGGGTCTGAAGGGCTTAAGTCAATCGGCCGATCACTTGGATTTGGGATTTCTCGTGCAGTGTTCCCTGAAGATCTTAAAGGGTCAGACAAGAAGATATATGATCCTCAGGACAAATTTCTCTTGTTGTGGAATCGATTTTTTGTCATCTCATGTATTCTGGCAGTGTCTGTGGACCCTCTGTTTTTCTATCTTCCAGTCATCAATAGTTCATCAAATTGTCTTGGTATAGATCGAAGGTTGGCTATCACAGCGACCACACTGCGGATGATTGTTGATGCTTTCTATCTTATTCACATGGCTCTCCAGTTTAGGACAGCTTACATTGCTCCATCATCTCGGGTTTTTGGAAGAGGTGAACTGGTGATTGATCCAGCACGAATAGCCAAGCGATACTTACGAAGTTATTTCATCATTGATTTTCTTTCAGTGCTACCCCTACCACAGATTGTAGTTTGGAGGTTTCTTCAGAGGTCCAAAGGTTCAGATGTGCTGGCTACAAAACAGGCTTTGCTTTACATAGTTTTATTTCAGTATATTCCCAGACTTTTTCGAGTCTATCCTTTGACTTCAGAACTGAAAAGAACAGCTGGTGTCTTTGCTGAAACTGCTTGGGCAGGTGCTGCATACTATTTGCTATTATATATGCTCGCTAGTCATATAGTTGGGGCATTCTGGTATTTGTTAGCTTTAGAACGCAATGATACATGCTGGCAGATGGCTTGTTCTGATTTTGGAAAACTATGTGATAAAAATCTCTTGTACTGCGGGAACCAAAACAATTTAGATAATGCAACTTGGTCCAATGTTACCGGAAACATCCTATCAAAATGCTCGGCAGATGACAATAATACCTACTTTGATTTTGGAATCTTTAATTCGGCTTTATCATCTGGGGTTGTTTCATCCAAGAAGTTCATCGTCAAATACTGTTACTGTTTATGGTGGGGACTACAGAATTTAAGTACGCTTGGTCAGGGTCTTGCAACCAGCACCTATCCTGGAGAGGTTATATTTTCCATATCACTGGCTATATTTGGACTTATCCTCTTCGCACTTTTGATAGGAAACATGCAGACCTATCTTCAGTCTCTTACTATTCGACTTGAGGAGATGAGGGTCAAAAGACGCGATTCAGAGCAGTGGATGCATCACCGCTTGCTCCCACAAGACCTTAGGGAACGTGTCAGGCGATATGATCAATATAAGTGGTTGGAAACACGCGGGGTGGACGAGCAGAGTTTGGTTCAGAGCCTACCAAAGGATCTCAGGAGAGATATTAAGCGGCACCTCTGTTTGGCATTAGTGAGGAGGGTTCCTTTGTTTGAGAATATGGACGAGAGGTTGCTTGATGCCATTTGTGAGAGACTGAAACCAAGTTTATTCACAGAGAGTACTTACATAGTTCGGGAAGGAGATCCCGTTGACGAGATGCTTTTCATCATACGTGGTCGCCTTGAGAGTGTAACAACAGATGGTGGCAGGAGTGGGTTTTTTAACCGAAGTTTACTGAAAGAAGGTGATTTCTGTGGTGAGGAGCTTCTAACTTGGGCACTGGATCCCAAATCTGGTGCCAACCTCCCGTCATCTACTCGGACAGTAAAGGCTTTAACTGAGGTTGAGGCCTTTGCTCTTGTAGCTGAAGAGTTGAAATTTGTTGCCAGTCAGTTTAGGCGCCTTCACAGCAGACAGGTTCAGCACACCTTCCGTTTCTACTCACAACAATGGCGGACTTGGGCTGCTTGCTTTATCCAAGCAGCATGGCGTCGCTATTCCAAGAGGAAAATCTTGGAGCTTCGTCGTAAGGAAGAGGAAGAGGAAGCAGAAGCAGAAGCCTTGGGAGGTGCTCGCAGCAATGCTGGAGGTTCATATAGCATTGGTGCCACATTTTTAGCTTCTAGGTTTGCTGCAAATGCACTTCGTGGTGTTCACCGAAACAGGAATTTGAAGAGTGCTCGAGAATTAGTCAAATTACAAAAGCCTCCGGAGCCCGATTTTAGCGCTGAAGGTGCCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005217 GO:0005221 GO:0005223 GO:0005244 GO:0005249 GO:0005261 GO:0005262 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006816 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015077 GO:0015079 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042391 GO:0043855 GO:0044425 GO:0044459 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071804 GO:0071805 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

736

Amino Acids

83.8

Weight (kDa)

9.32

Isoelectric Point (pI)

50.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	106 - 430	1.6e-22	Ion transport protein
cNMP_binding	PF00027	525 - 614	4.3e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002170)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT2G23980 AT4G30560 AT4G30560 AT4G30560 AT4G30560 AT4G30560 AT5G57940 AT5G57940 AT5G57940
fragaria_vesca	FvH4_1g00870 FvH4_1g00870 FvH4_1g00870 FvH4_1g00870
malus_domestica	MD02G1007200.v1.1 MD15G1144500.v1.1
prunus_persica	Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1 Prupe.7G263200_v2.0.a1
pyrus_communis	pycom02g00610 pycom02g00620 pycom15g13600 pycom15g13610
rosa_chinensis	RchiOBHm_Chr2g0085581
rosa_laevigata	RLG00000015703
rosa_multiflora	Rmu_co8217668.1_g000001 Rmu_ssc0000347.1_g000007
rosa_roxburghii	Rroxscaffold_2G00155250
rosa_rugosa	Rorug01G0461400
rosa_samantha	Rh2AG010500 Rh2BG011100 Rh2CG012000 Rh2DG013000
rosa_wichuraiana	Rw2G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	797
Acc36I	ACCTGC	1 cut(s)	797
AccB1I	GGYRCC	5 cut(s)	1476, 1760, 1863, 2069, 2198
AccII	CGCG	2 cut(s)	1320, 1413
AccIII	TCCGGA	1 cut(s)	2173
AciI	CCGC	6 cut(s)	454, 957, 1345, 1413, 1474, 1918
AclWI	GGATC	6 cut(s)	287, 554, 1461, 1601, 1742, 1755
AcoI	YGGCCR	1 cut(s)	217
AcsI	RAATTY	4 cut(s)	305, 1147, 1841, 2131
AcuI	CTGAAG	6 cut(s)	219, 279, 641, 744, 1259, 1851
AcyI	GRCGYC	2 cut(s)	1864, 1954
AdeI	CACNNNGTG	1 cut(s)	2111
AfaI	GTAC	4 cut(s)	32, 953, 1156, 1585
AfeI	AGCGCT	1 cut(s)	2191
AfiI	CCNNNNNNNGG	4 cut(s)	453, 1450, 1486, 1757
AflII	CTTAAG	1 cut(s)	206
AflIII	ACRYGT	1 cut(s)	1372
AgsI	TTSAA	2 cut(s)	1840, 2137
AjnI	CCWGG	1 cut(s)	1189
AluBI	AGCT	7 cut(s)	509, 779, 878, 1729, 1829, 1984, 2084
AluI	AGCT	7 cut(s)	509, 779, 878, 1729, 1829, 1984, 2084
Alw21I	GWGCWC	2 cut(s)	2038, 2146
Alw26I	GTCTC	3 cut(s)	42, 1284, 1549
AlwI	GGATC	6 cut(s)	287, 554, 1461, 1601, 1742, 1755
AlwNI	CAGNNNCTG	2 cut(s)	359, 812
Ama87I	CYCGRG	2 cut(s)	528, 2145
Aor13HI	TCCGGA	1 cut(s)	2173
Aor51HI	AGCGCT	1 cut(s)	2191
AoxI	GGCC	3 cut(s)	40, 217, 1812
ApeKI	GCWGC	5 cut(s)	812, 1928, 1946, 2040, 2096
ApoI	RAATTY	4 cut(s)	305, 1147, 1841, 2131
AspLEI	GCGC	2 cut(s)	1866, 2192
AspS9I	GGNCC	3 cut(s)	364, 663, 990
AsuC2I	CCSGG	1 cut(s)	145
AsuHPI	GGTGA	6 cut(s)	557, 565, 1334, 1721, 1733, 2111
AvaI	CYCGRG	2 cut(s)	528, 2145
AvaII	GGWCC	3 cut(s)	364, 663, 990
AxyI	CCTNAGG	2 cut(s)	297, 1364
BaeGI	GKGCMC	1 cut(s)	1744
BamHI	GGATCC	1 cut(s)	1747
BanI	GGYRCC	5 cut(s)	1476, 1760, 1863, 2069, 2198
BanII	GRGCYC	1 cut(s)	2181
BauI	CACGAG	1 cut(s)	243
Bbv12I	GWGCWC	2 cut(s)	2038, 2146
BbvI	GCAGC	5 cut(s)	799, 1915, 1958, 2052, 2083
BccI	CCATC	3 cut(s)	529, 901, 1658
BcgI	CGANNNNNNTGC	2 cut(s)	433, 467
BciT130I	CCWGG	1 cut(s)	1191
BciVI	GTATCC	1 cut(s)	157
BclI	TGATCA	1 cut(s)	1387
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	3 cut(s)	42, 1284, 1549
BfaI	CTAG	3 cut(s)	81, 843, 2088
BfmI	CTRYAG	1 cut(s)	1142
BfoI	RGCGCY	2 cut(s)	1867, 2193
BfrI	CTTAAG	1 cut(s)	206
BfuAI	ACCTGC	1 cut(s)	797
BfuI	GTATCC	1 cut(s)	157
BglII	AGATCT	1 cut(s)	262
BisI	GCNGC	6 cut(s)	813, 1475, 1929, 1947, 2041, 2097
BlsI	GCNGC	6 cut(s)	814, 1476, 1930, 1948, 2042, 2098
BmcAI	AGTACT	2 cut(s)	32, 1585
Bme1390I	CCNGG	2 cut(s)	145, 1191
Bme18I	GGWCC	3 cut(s)	364, 663, 990
BmeT110I	CYCGRG	2 cut(s)	528, 2145
BmgT120I	GGNCC	3 cut(s)	364, 663, 990
BmrFI	CCNGG	2 cut(s)	145, 1191
BmsI	GCATC	5 cut(s)	457, 1326, 1348, 1531, 1611
BpmI	CTGGAG	4 cut(s)	126, 479, 1212, 2070
BpuEI	CTTGAG	2 cut(s)	1316, 1667
BpuMI	CCSGG	1 cut(s)	145
Bsa29I	ATCGAT	1 cut(s)	325
BsaAI	YACGTR	1 cut(s)	1637
BsaBI	GATNNNNATC	2 cut(s)	88, 942
BsaHI	GRCGYC	2 cut(s)	1864, 1954
BsaJI	CCNNGG	1 cut(s)	2025
BsaWI	WCCGGW	2 cut(s)	1001, 2173
BsaXI	ACNNNNNCTCC	4 cut(s)	103, 133, 1596, 1626
Bsc4I	CCNNNNNNNGG	4 cut(s)	453, 1450, 1486, 1757
Bse1I	ACTGG	6 cut(s)	386, 496, 555, 1221, 1749, 1851
Bse21I	CCTNAGG	2 cut(s)	297, 1364
Bse3DI	GCAATG	3 cut(s)	513, 895, 2050
Bse8I	GATNNNNATC	2 cut(s)	88, 942
BseAI	TCCGGA	1 cut(s)	2173
BseBI	CCWGG	1 cut(s)	1191
BseCI	ATCGAT	1 cut(s)	325
BseDI	CCNNGG	1 cut(s)	2025
BseGI	GGATG	5 cut(s)	73, 462, 1008, 1094, 1341
BseJI	GATNNNNATC	2 cut(s)	88, 942
BseLI	CCNNNNNNNGG	4 cut(s)	453, 1450, 1486, 1757
BseMI	GCAATG	3 cut(s)	513, 895, 2050
BseMII	CTCAG	3 cut(s)	311, 1471, 1794
BseNI	ACTGG	6 cut(s)	386, 496, 555, 1221, 1749, 1851
BseRI	GAGGAG	3 cut(s)	1313, 1513, 1739
BseSI	GKGCMC	1 cut(s)	1744
BseX3I	CGGCCG	1 cut(s)	217
BseXI	GCAGC	5 cut(s)	799, 1915, 1958, 2052, 2083
BsgI	GTGCAG	1 cut(s)	267
Bsh1236I	CGCG	2 cut(s)	1320, 1413
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	3 cut(s)	42, 219, 1814
BshNI	GGYRCC	5 cut(s)	1476, 1760, 1863, 2069, 2198
BshVI	ATCGAT	1 cut(s)	325
BsiEI	CGRYCG	1 cut(s)	220
BsiHKAI	GWGCWC	2 cut(s)	2038, 2146
BsiHKCI	CYCGRG	2 cut(s)	528, 2145
BsiSI	CCGG	3 cut(s)	145, 1002, 2174
BslFI	GGGAC	2 cut(s)	150, 1152
BslI	CCNNNNNNNGG	4 cut(s)	453, 1450, 1486, 1757
BsmAI	GTCTC	3 cut(s)	42, 1284, 1549
BsmFI	GGGAC	2 cut(s)	150, 1152
BsmI	GAATGC	1 cut(s)	860
BsnI	GGCC	3 cut(s)	42, 219, 1814
BsoBI	CYCGRG	2 cut(s)	528, 2145
Bsp1286I	GDGCHC	4 cut(s)	1744, 2038, 2146, 2181
Bsp13I	TCCGGA	1 cut(s)	2173
Bsp143I	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
BspACI	CCGC	6 cut(s)	454, 957, 1345, 1413, 1474, 1918
BspANI	GGCC	3 cut(s)	42, 219, 1814
BspCNI	CTCAG	3 cut(s)	310, 1470, 1795
BspDI	ATCGAT	1 cut(s)	325
BspEI	TCCGGA	1 cut(s)	2173
BspFNI	CGCG	2 cut(s)	1320, 1413
BspMI	ACCTGC	1 cut(s)	797
BspPI	GGATC	6 cut(s)	287, 554, 1461, 1601, 1742, 1755
BspT107I	GGYRCC	5 cut(s)	1476, 1760, 1863, 2069, 2198
BspTI	CTTAAG	1 cut(s)	206
BsrDI	GCAATG	3 cut(s)	513, 895, 2050
BsrI	ACTGG	6 cut(s)	386, 496, 555, 1221, 1749, 1851
BssECI	CCNNGG	1 cut(s)	2025
BssMI	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
BssNI	GRCGYC	2 cut(s)	1864, 1954
BssSI	CACGAG	1 cut(s)	243
BssT1I	CCWWGG	1 cut(s)	2025
Bst2BI	CACGAG	1 cut(s)	243
Bst2UI	CCWGG	1 cut(s)	1191
Bst4CI	ACNGT	4 cut(s)	30, 1121, 1127, 1789
Bst6I	CTCTTC	6 cut(s)	535, 1244, 1827, 1992, 1998, 2132
BstACI	GRCGYC	2 cut(s)	1864, 1954
BstAFI	CTTAAG	1 cut(s)	206
BstAPI	GCANNNNNTGC	2 cut(s)	812, 1336
BstBAI	YACGTR	1 cut(s)	1637
BstC8I	GCNNGC	6 cut(s)	684, 840, 902, 1349, 1933, 2038
BstDEI	CTNAG	4 cut(s)	297, 1364, 1457, 1803
BstENI	CCTNNNNNAGG	1 cut(s)	1448
BstF5I	GGATG	5 cut(s)	73, 462, 1008, 1094, 1341
BstFNI	CGCG	2 cut(s)	1320, 1413
BstH2I	RGCGCY	2 cut(s)	1867, 2193
BstHHI	GCGC	2 cut(s)	1866, 2192
BstKTI	GATC	9 cut(s)	224, 265, 295, 424, 562, 1390, 1456, 1609, 1750
BstMAI	GTCTC	3 cut(s)	42, 1284, 1549
BstMBI	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
BstMCI	CGRYCG	1 cut(s)	220
BstNI	CCWGG	1 cut(s)	1191
BstNSI	RCATGY	2 cut(s)	900, 1264
BstSCI	CCNGG	2 cut(s)	143, 1189
BstSFI	CTRYAG	1 cut(s)	1142
BstSLI	GKGCMC	1 cut(s)	1744
BstUI	CGCG	2 cut(s)	1320, 1413
BstV1I	GCAGC	5 cut(s)	799, 1915, 1958, 2052, 2083
BstX2I	RGATCY	4 cut(s)	262, 1453, 1606, 1747
BstXI	CCANNNNNNTGG	1 cut(s)	1216
BstYI	RGATCY	4 cut(s)	262, 1453, 1606, 1747
BstZI	CGGCCG	1 cut(s)	217
Bsu15I	ATCGAT	1 cut(s)	325
Bsu36I	CCTNAGG	2 cut(s)	297, 1364
BsuI	GTATCC	1 cut(s)	157
BsuRI	GGCC	3 cut(s)	42, 219, 1814
BsuTUI	ATCGAT	1 cut(s)	325
BtgZI	GCGATG	1 cut(s)	75
BtsCI	GGATG	5 cut(s)	73, 462, 1008, 1094, 1341
BtsI	GCAGTG	4 cut(s)	255, 360, 449, 1337
BtsIMutI	CAGTG	7 cut(s)	255, 360, 449, 624, 1214, 1337, 1742
BveI	ACCTGC	1 cut(s)	797
Cac8I	GCNNGC	6 cut(s)	684, 840, 902, 1349, 1933, 2038
CaiI	CAGNNNCTG	2 cut(s)	359, 812
CfoI	GCGC	2 cut(s)	1866, 2192
Cfr13I	GGNCC	3 cut(s)	364, 663, 990
ClaI	ATCGAT	1 cut(s)	325
CseI	GACGC	2 cut(s)	1326, 1943
Csp6I	GTAC	4 cut(s)	31, 952, 1155, 1584
CspCI	CAANNNNNGTGG	2 cut(s)	1379, 1414
CviAII	CATG	5 cut(s)	342, 487, 897, 1261, 1950
CviQI	GTAC	4 cut(s)	31, 952, 1155, 1584
DdeI	CTNAG	4 cut(s)	297, 1364, 1457, 1803
DinI	GGCGCC	1 cut(s)	1865
DpnI	GATC	9 cut(s)	223, 264, 294, 423, 561, 1389, 1455, 1608, 1749
DpnII	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
DraIII	CACNNNGTG	1 cut(s)	2111
EaeI	YGGCCR	1 cut(s)	217
EagI	CGGCCG	1 cut(s)	217
Eam1104I	CTCTTC	6 cut(s)	535, 1244, 1827, 1992, 1998, 2132
EarI	CTCTTC	6 cut(s)	535, 1244, 1827, 1992, 1998, 2132
EciI	GGCGGA	1 cut(s)	1933
EclXI	CGGCCG	1 cut(s)	217
Eco130I	CCWWGG	1 cut(s)	2025
Eco147I	AGGCCT	1 cut(s)	1814
Eco24I	GRGCYC	1 cut(s)	2181
Eco47I	GGWCC	3 cut(s)	364, 663, 990
Eco47III	AGCGCT	1 cut(s)	2191
Eco52I	CGGCCG	1 cut(s)	217
Eco57I	CTGAAG	6 cut(s)	219, 279, 641, 744, 1259, 1851
Eco81I	CCTNAGG	2 cut(s)	297, 1364
Eco88I	CYCGRG	2 cut(s)	528, 2145
EcoNI	CCTNNNNNAGG	1 cut(s)	1448
EcoRII	CCWGG	1 cut(s)	1189
EcoT14I	CCWWGG	1 cut(s)	2025
EcoT22I	ATGCAT	1 cut(s)	1341
EcoT38I	GRGCYC	1 cut(s)	2181
EgeI	GGCGCC	1 cut(s)	1865
EheI	GGCGCC	1 cut(s)	1865
ErhI	CCWWGG	1 cut(s)	2025
FaeI	CATG	5 cut(s)	345, 490, 900, 1264, 1953
FalI	AAGNNNNNCTT	2 cut(s)	569, 601
FaqI	GGGAC	2 cut(s)	150, 1152
FatI	CATG	5 cut(s)	341, 486, 896, 1260, 1949
FauI	CCCGC	2 cut(s)	950, 1406
FbaI	TGATCA	1 cut(s)	1387
Fnu4HI	GCNGC	6 cut(s)	813, 1475, 1929, 1947, 2041, 2097
FokI	GGATG	5 cut(s)	80, 469, 995, 1081, 1348
FriOI	GRGCYC	1 cut(s)	2181
Fsp4HI	GCNGC	6 cut(s)	813, 1475, 1929, 1947, 2041, 2097
FspBI	CTAG	3 cut(s)	81, 843, 2088
GlaI	GCGC	2 cut(s)	1865, 2191
GluI	GCNGC	6 cut(s)	813, 1475, 1929, 1947, 2041, 2097
GsuI	CTGGAG	4 cut(s)	126, 479, 1212, 2070
HaeII	RGCGCY	2 cut(s)	1867, 2193
HaeIII	GGCC	3 cut(s)	42, 219, 1814
HapII	CCGG	3 cut(s)	145, 1002, 2174
HgaI	GACGC	2 cut(s)	1326, 1943
HhaI	GCGC	2 cut(s)	1866, 2192
Hin1I	GRCGYC	2 cut(s)	1864, 1954
Hin1II	CATG	5 cut(s)	345, 490, 900, 1264, 1953
Hin6I	GCGC	2 cut(s)	1864, 2190
HinP1I	GCGC	2 cut(s)	1864, 2190
HincII	GTYRAC	1 cut(s)	1615
HindII	GTYRAC	1 cut(s)	1615
HinfI	GANTC	6 cut(s)	77, 101, 322, 744, 1059, 1322
HpaII	CCGG	3 cut(s)	145, 1002, 2174
HphI	GGTGA	6 cut(s)	557, 565, 1334, 1721, 1733, 2111
Hpy166II	GTNNAC	6 cut(s)	364, 548, 1420, 1615, 1697, 2119
Hpy188III	TCNNGA	7 cut(s)	81, 105, 299, 1459, 1598, 2147, 2174
Hpy8I	GTNNAC	6 cut(s)	364, 548, 1420, 1615, 1697, 2119
Hpy99I	CGWCG	2 cut(s)	1959, 1992
HpyAV	CCTTC	8 cut(s)	105, 194, 420, 1595, 1700, 1877, 1903, 2189
HpyCH4III	ACNGT	4 cut(s)	30, 1121, 1127, 1789
HpyCH4IV	ACGT	2 cut(s)	1372, 1636
HpyCH4V	TGCA	9 cut(s)	158, 248, 815, 983, 1175, 1264, 1339, 2099, 2105
HpyF3I	CTNAG	4 cut(s)	297, 1364, 1457, 1803
HpySE526I	ACGT	2 cut(s)	1372, 1636
Hsp92I	GRCGYC	2 cut(s)	1864, 1954
Hsp92II	CATG	5 cut(s)	345, 490, 900, 1264, 1953
HspAI	GCGC	2 cut(s)	1864, 2190
KasI	GGCGCC	1 cut(s)	1863
Kpn2I	TCCGGA	1 cut(s)	2173
Ksp22I	TGATCA	1 cut(s)	1387
Kzo9I	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
LmnI	GCTCC	6 cut(s)	157, 523, 1356, 1726, 1981, 2176
Lsp1109I	GCAGC	5 cut(s)	799, 1915, 1958, 2052, 2083
LweI	GCATC	5 cut(s)	457, 1326, 1348, 1531, 1611
MaeI	CTAG	3 cut(s)	81, 843, 2088
MaeII	ACGT	2 cut(s)	1372, 1636
MaeIII	GTNAC	5 cut(s)	14, 24, 997, 1121, 1654
MalI	GATC	9 cut(s)	223, 264, 294, 423, 561, 1389, 1455, 1608, 1749
MboI	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
MflI	RGATCY	4 cut(s)	262, 1453, 1606, 1747
MhlI	GDGCHC	4 cut(s)	1744, 2038, 2146, 2181
MluCI	AATT	9 cut(s)	305, 406, 970, 1066, 1147, 1841, 2131, 2150, 2159
Mly113I	GGCGCC	1 cut(s)	1864
MlyI	GAGTC	2 cut(s)	71, 753
MmeI	TCCRAC	1 cut(s)	1383
Mph1103I	ATGCAT	1 cut(s)	1341
MroI	TCCGGA	1 cut(s)	2173
MseI	TTAA	9 cut(s)	126, 174, 207, 267, 1065, 1151, 1470, 1686, 1799
MslI	CAYNNNNRTG	1 cut(s)	1912
MspA1I	CMGCKG	1 cut(s)	779
MspCI	CTTAAG	1 cut(s)	206
MspI	CCGG	3 cut(s)	145, 1002, 2174
MspR9I	CCNGG	2 cut(s)	145, 1191
Mva1269I	GAATGC	1 cut(s)	860
MvaI	CCWGG	1 cut(s)	1191
MvnI	CGCG	2 cut(s)	1320, 1413
NarI	GGCGCC	1 cut(s)	1864
NciI	CCSGG	1 cut(s)	145
NdeII	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
NlaIII	CATG	5 cut(s)	345, 490, 900, 1264, 1953
NmeAIII	GCCGAG	1 cut(s)	1004
NmuCI	GTSAC	1 cut(s)	24
NsiI	ATGCAT	1 cut(s)	1341
NspI	RCATGY	2 cut(s)	900, 1264
PaeR7I	CTCGAG	1 cut(s)	2145
PaqCI	CACCTGC	1 cut(s)	797
PceI	AGGCCT	1 cut(s)	1814
PcsI	WCGNNNNNNNCGW	1 cut(s)	1378
PctI	GAATGC	1 cut(s)	860
PfeI	GAWTC	4 cut(s)	101, 322, 1059, 1322
PfoI	TCCNGGA	1 cut(s)	1189
PkrI	GCNGC	6 cut(s)	814, 1476, 1930, 1948, 2042, 2098
PleI	GAGTC	2 cut(s)	71, 752
PluTI	GGCGCC	1 cut(s)	1867
PpsI	GAGTC	2 cut(s)	71, 752
Ppu21I	YACGTR	1 cut(s)	1637
Psp6I	CCWGG	1 cut(s)	1189
PspGI	CCWGG	1 cut(s)	1189
PspPI	GGNCC	3 cut(s)	364, 663, 990
PstNI	CAGNNNCTG	2 cut(s)	359, 812
PsuI	RGATCY	4 cut(s)	262, 1453, 1606, 1747
PvuII	CAGCTG	1 cut(s)	779
RsaI	GTAC	4 cut(s)	32, 953, 1156, 1585
RsaNI	GTAC	4 cut(s)	31, 952, 1155, 1584
RseI	CAYNNNNRTG	1 cut(s)	1912
SaqAI	TTAA	9 cut(s)	126, 174, 207, 267, 1065, 1151, 1470, 1686, 1799
SatI	GCNGC	6 cut(s)	813, 1475, 1929, 1947, 2041, 2097
Sau3AI	GATC	9 cut(s)	221, 262, 292, 421, 559, 1387, 1453, 1606, 1747
Sau96I	GGNCC	3 cut(s)	364, 663, 990
ScaI	AGTACT	2 cut(s)	32, 1585
SchI	GAGTC	2 cut(s)	71, 753
ScrFI	CCNGG	2 cut(s)	145, 1191
SduI	GDGCHC	4 cut(s)	1744, 2038, 2146, 2181
SfaNI	GCATC	5 cut(s)	457, 1326, 1348, 1531, 1611
SfcI	CTRYAG	1 cut(s)	1142
SfoI	GGCGCC	1 cut(s)	1865
Sfr274I	CTCGAG	1 cut(s)	2145
SinI	GGWCC	3 cut(s)	364, 663, 990
SlaI	CTCGAG	1 cut(s)	2145
SmiMI	CAYNNNNRTG	1 cut(s)	1912
SmlI	CTYRAG	4 cut(s)	206, 1295, 1646, 2145
SmoI	CTYRAG	4 cut(s)	206, 1295, 1646, 2145
Sse9I	AATT	9 cut(s)	305, 406, 970, 1066, 1147, 1841, 2131, 2150, 2159
SseBI	AGGCCT	1 cut(s)	1814
SsiI	CCGC	6 cut(s)	454, 957, 1345, 1413, 1474, 1918
SspDI	GGCGCC	1 cut(s)	1863
SspI	AATATT	1 cut(s)	130
SspMI	CTAG	3 cut(s)	81, 843, 2088
StuI	AGGCCT	1 cut(s)	1814
StyD4I	CCNGG	2 cut(s)	143, 1189
StyI	CCWWGG	1 cut(s)	2025
TaaI	ACNGT	4 cut(s)	30, 1121, 1127, 1789
TaiI	ACGT	2 cut(s)	1375, 1639
TaqI	TCGA	5 cut(s)	325, 424, 742, 1291, 2146
TasI	AATT	9 cut(s)	305, 406, 970, 1066, 1147, 1841, 2131, 2150, 2159
TatI	WGTACW	3 cut(s)	30, 951, 1583
TauI	GCSGC	1 cut(s)	1477
TfiI	GAWTC	4 cut(s)	101, 322, 1059, 1322
Tru1I	TTAA	9 cut(s)	126, 174, 207, 267, 1065, 1151, 1470, 1686, 1799
Tru9I	TTAA	9 cut(s)	126, 174, 207, 267, 1065, 1151, 1470, 1686, 1799
TscAI	CASTG	7 cut(s)	255, 360, 456, 624, 1221, 1337, 1749
TseFI	GTSAC	1 cut(s)	24
TseI	GCWGC	5 cut(s)	812, 1928, 1946, 2040, 2096
Tsp45I	GTSAC	1 cut(s)	24
TspDTI	ATGAA	7 cut(s)	200, 390, 589, 1083, 1096, 1618, 2046
TspGWI	ACGGA	1 cut(s)	1886
TspRI	CASTG	7 cut(s)	255, 360, 456, 624, 1221, 1337, 1749
Vha464I	CTTAAG	1 cut(s)	206
VpaK11BI	GGWCC	3 cut(s)	364, 663, 990
XagI	CCTNNNNNAGG	1 cut(s)	1448
XapI	RAATTY	4 cut(s)	305, 1147, 1841, 2131
XbaI	TCTAGA	1 cut(s)	80
XceI	RCATGY	2 cut(s)	900, 1264
XhoI	CTCGAG	1 cut(s)	2145
XspI	CTAG	3 cut(s)	81, 843, 2088
ZrmI	AGTACT	2 cut(s)	32, 1585
Zsp2I	ATGCAT	1 cut(s)	1341

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.