Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009301

Physical Location & Seq

Genomic Coordinates Reverse (-)

496866 .. 502623

Gene Structure 5758 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g01010.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGGCCGACGTGTTCCGATCGGGTCAGTGCTCGGATCCGCCGCAGCTCAAATCGGCCGACGACGGGGCCTCGCCGTTTCCGAGGATCGAGTTTCATCTCGCGAGGAAGCCGTTCAAGGGGTTTAACAACGACGGCAGTGACTTCCGGCCCGAGACGCTTAATCCGGGAACTAGCGGCGGCTCCGATTCACGTCGACCCGGTTCGGGAGGTTCGGGTCAGGGCCAGTCGGGTTCGGGAGCGAAGAAGAGTGACGGGTCGGAGTTTGTGGAAAACGGGTGGGACCCGGAGCTTAGTTTTCCCATCACTTTTCGGAGAATTGGTGCCGGTTTGATAAACATGGGGAATACTTGTTATCTTAATTCGGTGTTGCAATGCCTGACGTATACTGAGCCTTTAGCTGCTTACTTGCAAAGTGGCAGGCATCGGAATTCGTGTCATATAAAGAATGGTTTTTGTGCTTTGTGTGCCATTCAAGAACACGTCAGTCTTGCTCTGCAATCGACTAAGAGCATTTTGCCCAAGGCTCTTGTCTTCAACCTACAACGCATATCGCGAAACTTTCGGAAGTCCAGACAGGAGGATGCACATGAATACATGGTAAACTTGCTGGAATCCATGCATAAATGTTGTTTACCATCTGGTGTGCCTAGTGAATCCCCTAGTGCCTATGAGAAGAGTTTGGTACACAAGATCTTTGGTGGTCGCCTACGAAGTCGGGTCAAATGTTTGCAGTGCTCGTATTGCTCTGACAAGTTAGATCCATTCCTAGATTTAAGTCTTGAAATAGTCAAGGCAAATTCTTTGAATAAAGCACTTGTGAACTTTACCTCCGAAGAACAACTGGATGGTGGGGAAAGACAATATCAGTGCCAAAAATGCAATCACAAAGTTCGAGCTAGCAAACAGATGACCATCCACAAGCCGCCTAGTGTTCTCACCATCCATTTAAAGCGGTTTCGTGCACATGACCCTGGACAAAAAATTAACAAAGAAGTTCAATTTGGTCCTACATTGGACCTGAGACCTTTTGTTAGTGGACCATATCCGGAAGGAGATTTGAAATATACTCTGTATGGGGTTCTAGTTCATTGTGGGGCTAGCACCTATTCTGGCCATTATTATTGCTTTGTTCGAACTTCAACTGGCATATGGCACTCTCTGGATGATAATAATGTTAGGCAAGTTCGTGAGATGACTGTTTTACAGCAGAAGGCTTACATGTTGTTCTATGTACGTGATAGAAGAAATATTATTCCGAGGAAGCCTGTTGATGTTGCTCAAATGGAGTATTTCAAACAAAATGGTATTGGAAATAAAATTACTCCTCCTGACAACCATTTGCCGAAAGAACCTATTCGAAAAATTGCAGCTGTGAACAGATCAAGTGACCTAGCCTCTTCTAGAGCGCAAAAAGATGCATCAGCTATAGTCACAAGGGTTTCCCATTTGAAGGAAGCATCAGTTGATCCAAATAATGGCCACATAGTGACACAAAATATGGTGCATAAGGAGGCTATTTTAGAGTCGTCTTCAAAAGCGTCATTATCAGAGGGCTCGTTGGGACAAAATATGCCACCATGTAATCCGCTTCCAAGCAGCAAAACTGGCACGTCTGATTCTGCAAGTGGCGGGGATTCAACAACTGATGCCAAAATTAATGAATGCAATGAGAAAGCAAGTTCAAATGATAATGACAGGGTTTCGATTGTGATATTGCCGACTGTGAAGGACCCTGAAACCTTAAAGGCTTGTAAGCCTGTTCAAGATGAGATCTCTCAGAATAATCATGCTCCCAGTGCTGGGAATTTGAGTGTGGCTTCATCCACAATTCCTAGTGGTTTACCATTGGAGAAATTGAATCCTGAAAAGCCATCTGATCCACCAAGCTCTAGAATCAGTCAGGCTGGAAGTTTTATCAATGAACGCGAGAAGGGTAGTGATTCTGGCCAGATAGTTGTAGTTGCTGATTCAGCAAAATTGGTTGACTCATTAGTTTTGACAGATAGATCATTAGATGTTGAAGCTCCTGACCGCAAGCAACCTAAAGGATTGAAAAAGAAGCACATAAAGTGTAAGGTGGCAGGCATGCATATGCATCCATCCTTGCTTTCTAGGGCAGTCTTAAGGGTGCAGAAGAAGAAGCACAAAAGTAGTAAACACCCAACTTCAGATCGTAAGAGCCATAGTAAGAAATACTTATTAGAAAACTGTTGTTCTTCATCTGATCAAGGACCATCTACATCTGAGAAAACCCAGACAGATTCCTTGGTTTCTGTCTGTTCGAAGAGAAAGAGAAACAAATCTGGCTTGAAAACGGATGAAGATGGAACTTCTGATAAGTACTTAGTGAATTCTCGTGGTGATTGTTCGATGGATATTAAAGATGTTGAGTTAAGAGGGAGCCTCATTCATAATGGAACAGTGCTTGCAAATGATAAACTACAACAAAACGGTCTTGGCTCTTCCTCTGTTACACTGGAAAACCAAAGGGAGTCGAGGGGAACCGATGCTCCACAAAATTGTAAAAGAGATGAAATGCAAAATGGATGGAAGGTTGTACTGACTCAAGGTCCAGCTGAGACAATTGTTGCACCTTGGGATGGAATAGAATTGCCTCAGTCTCATGTTGTAGAATCAAGCCACGCACCTGTCACCATCGGTTATGTTGGAGATGAATGGGATGAAGAATATGATCGGGGGAAGAGAAAGAAGGTAAGGCAAGCAAAACTCAGCCTGGGAGGATCAAATCCTTTCCAAATGCTGGCAAACAAGAAAGCACAATTGAAGAAACCAAGGATACAAAGGTTTGATTCTGGAAATCGTCCACTTAGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH

Protein Analysis

946

Amino Acids

103.97

Weight (kDa)

9.2

Isoelectric Point (pI)

49.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	108 - 410	1.7e-62	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	108 - 391	1.4e-15	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57990
fragaria_vesca	FvH4_1g01010 FvH4_1g01010 FvH4_1g01010
malus_domestica	MD02G1008300.v1.1 MD15G1152500.v1.1
prunus_persica	Prupe.7G262200_v2.0.a1
pyrus_communis	pycom02g00720 pycom15g13710
rosa_chinensis	RchiOBHm_Chr2g0085751
rosa_laevigata	RLG00000015716
rosa_multiflora	Rmu_sc0009759.1_g000004 Rmu_sc0023131.1_g000003 Rmu_sc0033740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155070
rosa_rugosa	Rorug01G0462900 Rorug01G0462900
rosa_samantha	Rh2AG012000 Rh2BG012600 Rh2CG013400 Rh2DG014400
rosa_wichuraiana	Rw2G001070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	320
AccB7I	CCANNNNNTGG	2 cut(s)	1475, 2761
AccI	GTMKAC	2 cut(s)	193, 383
AccII	CGCG	3 cut(s)	101, 553, 1926
AccIII	TCCGGA	1 cut(s)	1045
AciI	CCGC	9 cut(s)	38, 41, 174, 177, 923, 952, 1586, 1629, 2032
AclWI	GGATC	7 cut(s)	29, 42, 92, 752, 1460, 1871, 2749
AcoI	YGGCCR	5 cut(s)	3, 54, 1111, 1477, 1945
AcsI	RAATTY	4 cut(s)	427, 796, 1804, 2350
AcuI	CTGAAG	1 cut(s)	2151
AdeI	CACNNNGTG	1 cut(s)	1486
AfaI	GTAC	4 cut(s)	684, 1233, 2342, 2559
AfiI	CCNNNNNNNGG	9 cut(s)	63, 116, 203, 1450, 1475, 1799, 1800, 2600, 2761
AflII	CTTAAG	1 cut(s)	2122
AflIII	ACRYGT	3 cut(s)	9, 478, 1218
AhdI	GACNNNNNGTC	1 cut(s)	2568
AjiI	CACGTC	4 cut(s)	10, 191, 481, 1611
AjnI	CCWGG	2 cut(s)	970, 2733
AjuI	GAANNNNNNNTTGG	2 cut(s)	984, 1016
AloI	GAACNNNNNNTCC	4 cut(s)	812, 844, 2494, 2526
AluBI	AGCT	9 cut(s)	46, 289, 398, 896, 1370, 1424, 1887, 2024, 2576
AluI	AGCT	9 cut(s)	46, 289, 398, 896, 1370, 1424, 1887, 2024, 2576
Alw21I	GWGCWC	3 cut(s)	32, 737, 964
Alw26I	GTCTC	4 cut(s)	146, 1015, 2573, 2625
Alw44I	GTGCAC	1 cut(s)	960
AlwI	GGATC	7 cut(s)	29, 42, 92, 752, 1460, 1871, 2749
Ama87I	CYCGRG	1 cut(s)	149
Aor13HI	TCCGGA	1 cut(s)	1045
AoxI	GGCC	8 cut(s)	3, 54, 66, 146, 220, 1111, 1477, 1945
ApaLI	GTGCAC	1 cut(s)	960
ApeKI	GCWGC	4 cut(s)	43, 398, 1367, 1596
ApoI	RAATTY	4 cut(s)	427, 796, 1804, 2350
AseI	ATTAAT	1 cut(s)	1656
Asp700I	GAANNNNTTC	2 cut(s)	110, 1353
AspLEI	GCGC	1 cut(s)	1408
AsuC2I	CCSGG	3 cut(s)	165, 198, 284
AsuHPI	GGTGA	3 cut(s)	928, 2372, 2644
AsuII	TTCGAA	3 cut(s)	1132, 1357, 2282
AsuNHI	GCTAGC	2 cut(s)	896, 1097
AvaI	CYCGRG	1 cut(s)	149
AvaII	GGWCC	7 cut(s)	280, 1004, 1015, 1037, 1729, 2231, 2570
BaeGI	GKGCMC	1 cut(s)	964
BaeI	ACNNNNGTAYC	2 cut(s)	2789, 2822
BalI	TGGCCA	3 cut(s)	1113, 1479, 1947
BamHI	GGATCC	1 cut(s)	34
BanI	GGYRCC	1 cut(s)	320
BanII	GRGCYC	1 cut(s)	1556
BauI	CACGAG	1 cut(s)	2355
BbsI	GAAGAC	2 cut(s)	523, 1521
Bbv12I	GWGCWC	3 cut(s)	32, 737, 964
BbvI	GCAGC	4 cut(s)	55, 385, 1379, 1608
BceAI	ACGGC	3 cut(s)	58, 94, 148
BciT130I	CCWGG	2 cut(s)	972, 2735
BciVI	GTATCC	1 cut(s)	2790
BclI	TGATCA	1 cut(s)	2224
BcnI	CCSGG	3 cut(s)	165, 198, 284
BcoDI	GTCTC	4 cut(s)	146, 1015, 2573, 2625
BfmI	CTRYAG	1 cut(s)	1425
BfrI	CTTAAG	1 cut(s)	2122
BfuI	GTATCC	1 cut(s)	2790
BglII	AGATCT	2 cut(s)	690, 1770
BisI	GCNGC	8 cut(s)	41, 44, 175, 178, 399, 923, 1368, 1597
BlsI	GCNGC	8 cut(s)	42, 45, 176, 179, 400, 924, 1369, 1598
BmcAI	AGTACT	1 cut(s)	2342
Bme1390I	CCNGG	5 cut(s)	165, 198, 284, 972, 2735
Bme18I	GGWCC	7 cut(s)	280, 1004, 1015, 1037, 1729, 2231, 2570
BmeRI	GACNNNNNGTC	1 cut(s)	2568
BmeT110I	CYCGRG	1 cut(s)	149
BmgBI	CACGTC	4 cut(s)	10, 191, 481, 1611
BmiI	GGNNCC	9 cut(s)	36, 67, 181, 281, 282, 322, 1731, 2402, 2503
BmrFI	CCNGG	5 cut(s)	165, 198, 284, 972, 2735
BmrI	ACTGGG	1 cut(s)	1788
BmsI	GCATC	8 cut(s)	430, 571, 1405, 1427, 1466, 1636, 2104, 2497
BmtI	GCTAGC	2 cut(s)	900, 1101
BmuI	ACTGGG	1 cut(s)	1788
BpiI	GAAGAC	2 cut(s)	523, 1521
BplI	GAGNNNNNCTC	2 cut(s)	2388, 2420
Bpu14I	TTCGAA	3 cut(s)	1132, 1357, 2282
BpuEI	CTTGAG	1 cut(s)	2550
BpuMI	CCSGG	3 cut(s)	165, 198, 284
BsaAI	YACGTR	1 cut(s)	1235
BsaBI	GATNNNNATC	1 cut(s)	911
BsaI	GGTCTC	1 cut(s)	1015
BsaJI	CCNNGG	8 cut(s)	80, 519, 970, 1256, 2265, 2594, 2734, 2792
BsaWI	WCCGGW	1 cut(s)	1045
BsaXI	ACNNNNNCTCC	6 cut(s)	304, 334, 812, 842, 2494, 2524
Bsc4I	CCNNNNNNNGG	9 cut(s)	63, 116, 203, 1450, 1475, 1799, 1800, 2600, 2761
Bse118I	RCCGGY	1 cut(s)	323
Bse1I	ACTGG	6 cut(s)	223, 846, 1147, 1609, 1794, 2481
Bse3DI	GCAATG	2 cut(s)	377, 1672
Bse8I	GATNNNNATC	1 cut(s)	911
BseAI	TCCGGA	1 cut(s)	1045
BseBI	CCWGG	2 cut(s)	972, 2735
BseDI	CCNNGG	8 cut(s)	80, 519, 970, 1256, 2265, 2594, 2734, 2792
BseJI	GATNNNNATC	1 cut(s)	911
BseLI	CCNNNNNNNGG	9 cut(s)	63, 116, 203, 1450, 1475, 1799, 1800, 2600, 2761
BseMI	GCAATG	2 cut(s)	377, 1672
BseMII	CTCAG	7 cut(s)	378, 1010, 1790, 2235, 2568, 2630, 2743
BseNI	ACTGG	6 cut(s)	223, 846, 1147, 1609, 1794, 2481
BseRI	GAGGAG	1 cut(s)	1314
BseSI	GKGCMC	1 cut(s)	964
BseX3I	CGGCCG	1 cut(s)	54
BseXI	GCAGC	4 cut(s)	55, 385, 1379, 1608
BseYI	CCCAGC	1 cut(s)	1799
BsgI	GTGCAG	1 cut(s)	2150
Bsh1236I	CGCG	3 cut(s)	101, 553, 1926
Bsh1285I	CGRYCG	2 cut(s)	20, 57
BshFI	GGCC	8 cut(s)	5, 56, 68, 148, 222, 1113, 1479, 1947
BshNI	GGYRCC	1 cut(s)	320
BsiEI	CGRYCG	2 cut(s)	20, 57
BsiHKAI	GWGCWC	3 cut(s)	32, 737, 964
BsiHKCI	CYCGRG	1 cut(s)	149
BsiSI	CCGG	6 cut(s)	145, 164, 198, 284, 324, 1046
BslFI	GGGAC	2 cut(s)	293, 1575
BslI	CCNNNNNNNGG	9 cut(s)	63, 116, 203, 1450, 1475, 1799, 1800, 2600, 2761
BsmAI	GTCTC	4 cut(s)	146, 1015, 2573, 2625
BsmBI	CGTCTC	1 cut(s)	146
BsmFI	GGGAC	2 cut(s)	293, 1575
BsmI	GAATGC	1 cut(s)	1667
BsnI	GGCC	8 cut(s)	5, 56, 68, 148, 222, 1113, 1479, 1947
Bso31I	GGTCTC	1 cut(s)	1015
BsoBI	CYCGRG	1 cut(s)	149
Bsp119I	TTCGAA	3 cut(s)	1132, 1357, 2282
Bsp1286I	GDGCHC	4 cut(s)	32, 737, 964, 1556
Bsp13I	TCCGGA	1 cut(s)	1045
Bsp68I	TCGCGA	2 cut(s)	101, 553
BspACI	CCGC	9 cut(s)	38, 41, 174, 177, 923, 952, 1586, 1629, 2032
BspANI	GGCC	8 cut(s)	5, 56, 68, 148, 222, 1113, 1479, 1947
BspCNI	CTCAG	7 cut(s)	379, 1011, 1789, 2236, 2569, 2629, 2742
BspEI	TCCGGA	1 cut(s)	1045
BspFNI	CGCG	3 cut(s)	101, 553, 1926
BspLI	GGNNCC	9 cut(s)	36, 67, 181, 281, 282, 322, 1731, 2402, 2503
BspOI	GCTAGC	2 cut(s)	900, 1101
BspPI	GGATC	7 cut(s)	29, 42, 92, 752, 1460, 1871, 2749
BspQI	GCTCTTC	1 cut(s)	2467
BspT104I	TTCGAA	3 cut(s)	1132, 1357, 2282
BspT107I	GGYRCC	1 cut(s)	320
BspTI	CTTAAG	1 cut(s)	2122
BspTNI	GGTCTC	1 cut(s)	1015
BsrDI	GCAATG	2 cut(s)	377, 1672
BsrFI	RCCGGY	1 cut(s)	323
BsrI	ACTGG	6 cut(s)	223, 846, 1147, 1609, 1794, 2481
BssAI	RCCGGY	1 cut(s)	323
BssECI	CCNNGG	8 cut(s)	80, 519, 970, 1256, 2265, 2594, 2734, 2792
BssNAI	GTATAC	1 cut(s)	384
BssSI	CACGAG	1 cut(s)	2355
BssT1I	CCWWGG	4 cut(s)	519, 2265, 2594, 2792
Bst1107I	GTATAC	1 cut(s)	384
Bst2BI	CACGAG	1 cut(s)	2355
Bst2UI	CCWGG	2 cut(s)	972, 2735
Bst4CI	ACNGT	5 cut(s)	1198, 1723, 2210, 2422, 2453
Bst6I	CTCTTC	6 cut(s)	239, 668, 1402, 2279, 2467, 2696
BstAFI	CTTAAG	1 cut(s)	2122
BstBAI	YACGTR	1 cut(s)	1235
BstBI	TTCGAA	3 cut(s)	1132, 1357, 2282
BstC8I	GCNNGC	9 cut(s)	419, 898, 1099, 2036, 2083, 2087, 2427, 2721, 2763
BstFNI	CGCG	3 cut(s)	101, 553, 1926
BstHHI	GCGC	1 cut(s)	1408
BstMAI	GTCTC	4 cut(s)	146, 1015, 2573, 2625
BstMCI	CGRYCG	2 cut(s)	20, 57
BstMWI	GCNNNNNNNGC	7 cut(s)	154, 464, 741, 876, 1605, 1796, 1970
BstNI	CCWGG	2 cut(s)	972, 2735
BstNSI	RCATGY	2 cut(s)	1222, 2089
BstSCI	CCNGG	5 cut(s)	163, 196, 282, 970, 2733
BstSFI	CTRYAG	1 cut(s)	1425
BstSLI	GKGCMC	1 cut(s)	964
BstUI	CGCG	3 cut(s)	101, 553, 1926
BstV1I	GCAGC	4 cut(s)	55, 385, 1379, 1608
BstV2I	GAAGAC	2 cut(s)	523, 1521
BstX2I	RGATCY	4 cut(s)	34, 690, 757, 1770
BstYI	RGATCY	4 cut(s)	34, 690, 757, 1770
BstZ17I	GTATAC	1 cut(s)	384
BstZI	CGGCCG	1 cut(s)	54
BsuI	GTATCC	1 cut(s)	2790
BsuRI	GGCC	8 cut(s)	5, 56, 68, 148, 222, 1113, 1479, 1947
BtrI	CACGTC	4 cut(s)	10, 191, 481, 1611
BtsI	GCAGTG	2 cut(s)	142, 737
BtsIMutI	CAGTG	7 cut(s)	32, 142, 737, 872, 1801, 2427, 2474
BtuMI	TCGCGA	2 cut(s)	101, 553
Cac8I	GCNNGC	9 cut(s)	419, 898, 1099, 2036, 2083, 2087, 2427, 2721, 2763
CfoI	GCGC	1 cut(s)	1408
Cfr10I	RCCGGY	1 cut(s)	323
CseI	GACGC	2 cut(s)	163, 1527
Csp6I	GTAC	4 cut(s)	683, 1232, 2341, 2558
CviQI	GTAC	4 cut(s)	683, 1232, 2341, 2558
DraI	TTTAAA	1 cut(s)	948
DraIII	CACNNNGTG	1 cut(s)	1486
DriI	GACNNNNNGTC	1 cut(s)	2568
EaeI	YGGCCR	5 cut(s)	3, 54, 1111, 1477, 1945
EagI	CGGCCG	1 cut(s)	54
Eam1104I	CTCTTC	6 cut(s)	239, 668, 1402, 2279, 2467, 2696
Eam1105I	GACNNNNNGTC	1 cut(s)	2568
EarI	CTCTTC	6 cut(s)	239, 668, 1402, 2279, 2467, 2696
EciI	GGCGGA	1 cut(s)	27
EclXI	CGGCCG	1 cut(s)	54
Eco130I	CCWWGG	4 cut(s)	519, 2265, 2594, 2792
Eco24I	GRGCYC	1 cut(s)	1556
Eco31I	GGTCTC	1 cut(s)	1015
Eco47I	GGWCC	7 cut(s)	280, 1004, 1015, 1037, 1729, 2231, 2570
Eco52I	CGGCCG	1 cut(s)	54
Eco57I	CTGAAG	1 cut(s)	2151
Eco88I	CYCGRG	1 cut(s)	149
EcoO109I	RGGNCCY	3 cut(s)	66, 280, 1729
EcoRI	GAATTC	2 cut(s)	427, 2350
EcoRII	CCWGG	2 cut(s)	970, 2733
EcoT14I	CCWWGG	4 cut(s)	519, 2265, 2594, 2792
EcoT22I	ATGCAT	4 cut(s)	621, 1420, 2091, 2097
EcoT38I	GRGCYC	1 cut(s)	1556
ErhI	CCWWGG	4 cut(s)	519, 2265, 2594, 2792
Esp3I	CGTCTC	1 cut(s)	146
FalI	AAGNNNNNCTT	2 cut(s)	2180, 2212
FaqI	GGGAC	2 cut(s)	293, 1575
FauI	CCCGC	1 cut(s)	1622
FauNDI	CATATG	2 cut(s)	1148, 2091
FbaI	TGATCA	1 cut(s)	2224
FblI	GTMKAC	2 cut(s)	193, 383
Fnu4HI	GCNGC	8 cut(s)	41, 44, 175, 178, 399, 923, 1368, 1597
FriOI	GRGCYC	1 cut(s)	1556
Fsp4HI	GCNGC	8 cut(s)	41, 44, 175, 178, 399, 923, 1368, 1597
GlaI	GCGC	1 cut(s)	1407
GluI	GCNGC	8 cut(s)	41, 44, 175, 178, 399, 923, 1368, 1597
GsaI	CCCAGC	1 cut(s)	1803
HaeIII	GGCC	8 cut(s)	5, 56, 68, 148, 222, 1113, 1479, 1947
HapII	CCGG	6 cut(s)	145, 164, 198, 284, 324, 1046
HgaI	GACGC	2 cut(s)	163, 1527
HhaI	GCGC	1 cut(s)	1408
Hin6I	GCGC	1 cut(s)	1406
HinP1I	GCGC	1 cut(s)	1406
HincII	GTYRAC	2 cut(s)	194, 1984
HindII	GTYRAC	2 cut(s)	194, 1984
HpaII	CCGG	6 cut(s)	145, 164, 198, 284, 324, 1046
HphI	GGTGA	3 cut(s)	928, 2372, 2644
Hpy99I	CGWCG	5 cut(s)	11, 62, 65, 134, 195
HpyAV	CCTTC	7 cut(s)	1043, 1204, 1444, 1720, 1924, 2545, 2704
HpyCH4III	ACNGT	5 cut(s)	1198, 1723, 2210, 2422, 2453
HpyCH4IV	ACGT	6 cut(s)	9, 190, 380, 480, 1234, 1610
HpyF10VI	GCNNNNNNNGC	7 cut(s)	154, 464, 741, 876, 1605, 1796, 1970
HpySE526I	ACGT	6 cut(s)	9, 190, 380, 480, 1234, 1610
HspAI	GCGC	1 cut(s)	1406
KflI	GGGWCCC	1 cut(s)	280
Kpn2I	TCCGGA	1 cut(s)	1045
Ksp22I	TGATCA	1 cut(s)	2224
LguI	GCTCTTC	1 cut(s)	2467
LmnI	GCTCC	7 cut(s)	185, 236, 286, 1795, 2029, 2400, 2515
Lsp1109I	GCAGC	4 cut(s)	55, 385, 1379, 1608
LweI	GCATC	8 cut(s)	430, 571, 1405, 1427, 1466, 1636, 2104, 2497
MaeII	ACGT	6 cut(s)	9, 190, 380, 480, 1234, 1610
MaeIII	GTNAC	7 cut(s)	137, 248, 1385, 1429, 1486, 2470, 2650
MfeI	CAATTG	2 cut(s)	2583, 2780
MflI	RGATCY	4 cut(s)	34, 690, 757, 1770
MhlI	GDGCHC	4 cut(s)	32, 737, 964, 1556
MlsI	TGGCCA	3 cut(s)	1113, 1479, 1947
MluNI	TGGCCA	3 cut(s)	1113, 1479, 1947
MlyI	GAGTC	4 cut(s)	1532, 1979, 2501, 2557
MmeI	TCCRAC	2 cut(s)	237, 2647
Mox20I	TGGCCA	3 cut(s)	1113, 1479, 1947
Mph1103I	ATGCAT	4 cut(s)	621, 1420, 2091, 2097
MroI	TCCGGA	1 cut(s)	1045
MroXI	GAANNNNTTC	2 cut(s)	110, 1353
MscI	TGGCCA	3 cut(s)	1113, 1479, 1947
MslI	CAYNNNNRTG	1 cut(s)	2090
Msp20I	TGGCCA	3 cut(s)	1113, 1479, 1947
MspA1I	CMGCKG	2 cut(s)	1370, 2576
MspCI	CTTAAG	1 cut(s)	2122
MspI	CCGG	6 cut(s)	145, 164, 198, 284, 324, 1046
MspR9I	CCNGG	5 cut(s)	165, 198, 284, 972, 2735
MunI	CAATTG	2 cut(s)	2583, 2780
Mva1269I	GAATGC	1 cut(s)	1667
MvaI	CCWGG	2 cut(s)	972, 2735
MvnI	CGCG	3 cut(s)	101, 553, 1926
MwoI	GCNNNNNNNGC	7 cut(s)	154, 464, 741, 876, 1605, 1796, 1970
NciI	CCSGG	3 cut(s)	165, 198, 284
NdeI	CATATG	2 cut(s)	1148, 2091
NheI	GCTAGC	2 cut(s)	896, 1097
NlaIV	GGNNCC	9 cut(s)	36, 67, 181, 281, 282, 322, 1731, 2402, 2503
NmuCI	GTSAC	6 cut(s)	137, 248, 1385, 1429, 1486, 2650
NruI	TCGCGA	2 cut(s)	101, 553
NsiI	ATGCAT	4 cut(s)	621, 1420, 2091, 2097
NspI	RCATGY	2 cut(s)	1222, 2089
NspV	TTCGAA	3 cut(s)	1132, 1357, 2282
PaeI	GCATGC	1 cut(s)	2089
PciI	ACATGT	1 cut(s)	1218
PciSI	GCTCTTC	1 cut(s)	2467
PcsI	WCGNNNNNNNCGW	2 cut(s)	77, 550
PctI	GAATGC	1 cut(s)	1667
PdmI	GAANNNNTTC	2 cut(s)	110, 1353
PflMI	CCANNNNNTGG	2 cut(s)	1475, 2761
PfoI	TCCNGGA	1 cut(s)	163
PkrI	GCNGC	8 cut(s)	42, 45, 176, 179, 400, 924, 1369, 1598
Ple19I	CGATCG	1 cut(s)	20
PleI	GAGTC	4 cut(s)	1531, 1979, 2500, 2557
PpsI	GAGTC	4 cut(s)	1531, 1979, 2500, 2557
Ppu21I	YACGTR	1 cut(s)	1235
PpuMI	RGGWCCY	2 cut(s)	280, 1729
PscI	ACATGT	1 cut(s)	1218
PshBI	ATTAAT	1 cut(s)	1656
Psp5II	RGGWCCY	2 cut(s)	280, 1729
Psp6I	CCWGG	2 cut(s)	970, 2733
PspFI	CCCAGC	1 cut(s)	1799
PspGI	CCWGG	2 cut(s)	970, 2733
PspN4I	GGNNCC	9 cut(s)	36, 67, 181, 281, 282, 322, 1731, 2402, 2503
PspPPI	RGGWCCY	2 cut(s)	280, 1729
PsuI	RGATCY	4 cut(s)	34, 690, 757, 1770
PvuI	CGATCG	1 cut(s)	20
PvuII	CAGCTG	2 cut(s)	1370, 2576
RruI	TCGCGA	2 cut(s)	101, 553
RsaI	GTAC	4 cut(s)	684, 1233, 2342, 2559
RsaNI	GTAC	4 cut(s)	683, 1232, 2341, 2558
RseI	CAYNNNNRTG	1 cut(s)	2090
SalI	GTCGAC	1 cut(s)	192
SapI	GCTCTTC	1 cut(s)	2467
SatI	GCNGC	8 cut(s)	41, 44, 175, 178, 399, 923, 1368, 1597
ScaI	AGTACT	1 cut(s)	2342
SchI	GAGTC	4 cut(s)	1532, 1979, 2501, 2557
ScrFI	CCNGG	5 cut(s)	165, 198, 284, 972, 2735
SduI	GDGCHC	4 cut(s)	32, 737, 964, 1556
SfaNI	GCATC	8 cut(s)	430, 571, 1405, 1427, 1466, 1636, 2104, 2497
SfcI	CTRYAG	1 cut(s)	1425
SfuI	TTCGAA	3 cut(s)	1132, 1357, 2282
SinI	GGWCC	7 cut(s)	280, 1004, 1015, 1037, 1729, 2231, 2570
SmiMI	CAYNNNNRTG	1 cut(s)	2090
SmlI	CTYRAG	2 cut(s)	2122, 2565
SmoI	CTYRAG	2 cut(s)	2122, 2565
SphI	GCATGC	1 cut(s)	2089
SsiI	CCGC	9 cut(s)	38, 41, 174, 177, 923, 952, 1586, 1629, 2032
SspI	AATATT	1 cut(s)	1249
StyD4I	CCNGG	5 cut(s)	163, 196, 282, 970, 2733
StyI	CCWWGG	4 cut(s)	519, 2265, 2594, 2792
TaaI	ACNGT	5 cut(s)	1198, 1723, 2210, 2422, 2453
TaiI	ACGT	6 cut(s)	12, 193, 383, 483, 1237, 1613
TatI	WGTACW	2 cut(s)	2340, 2557
TauI	GCSGC	4 cut(s)	43, 177, 180, 925
TscAI	CASTG	7 cut(s)	32, 142, 737, 872, 1801, 2427, 2481
TseFI	GTSAC	6 cut(s)	137, 248, 1385, 1429, 1486, 2650
TseI	GCWGC	4 cut(s)	43, 398, 1367, 1596
Tsp45I	GTSAC	6 cut(s)	137, 248, 1385, 1429, 1486, 2650
TspGWI	ACGGA	1 cut(s)	2330
TspRI	CASTG	7 cut(s)	32, 142, 737, 872, 1801, 2427, 2481
Van91I	CCANNNNNTGG	2 cut(s)	1475, 2761
Vha464I	CTTAAG	1 cut(s)	2122
VneI	GTGCAC	1 cut(s)	960
VpaK11BI	GGWCC	7 cut(s)	280, 1004, 1015, 1037, 1729, 2231, 2570
VspI	ATTAAT	1 cut(s)	1656
XapI	RAATTY	4 cut(s)	427, 796, 1804, 2350
XbaI	TCTAGA	2 cut(s)	1400, 1889
XceI	RCATGY	2 cut(s)	1222, 2089
XmiI	GTMKAC	2 cut(s)	193, 383
XmnI	GAANNNNTTC	2 cut(s)	110, 1353
ZrmI	AGTACT	1 cut(s)	2342
Zsp2I	ATGCAT	4 cut(s)	621, 1420, 2091, 2097

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.