Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009301

Physical Location & Seq

Genomic Coordinates Reverse (-)

516110 .. 522906

Gene Structure 6797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1008300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGGCGGAGCTCCAAAGACAATCGGATGGGTTTGCGGATCTAATGGCCGAATCGGAAACTGGGTCAAATCCGCTTCAGAGGCGTATTGAGTTCCACCGAGCGAGGAAGCCCTTCAATGGGTTTAACACTGGCGGCGGTGACTTCCGGCTCGAGACCTTGAATCACGGAAGCAGCGGCGGAAGCAGCTCTAGTCAGGGTCAGTCGGGTTTGAGTGCCAAAAAAAGGGATGGGTCCGAGTTTTTGGAGAACGGGTTCGATCCGGAGCTCAGCTTTGGCATCACCTTCCGGAGAATTGGTGCCGGTTTGAGGAATATGGGAAACACTTGTTATCTCAATTCAGTGTTGCAGTGTCTGACATATACTGAGCCTTTAGCTGCATACTTGCAAAGTGGAAAGCATCGAAATTCATGCCATATTGCTGGGTTTTGTGCTTTATGTGCTATCCAGAAGCATGTTAGTCTTGCTCTACAATCCACTGGGAGAATTTTGGTGCCAAAGGATCTAGTCATTAACTTACAATGCATATCTCGAACTTTCACTAAGTCTAGACAGGAGGATGCACATGAATACATGGTAAACTTGCTGGAGTCCATGCATAAATGCTGTTTACCGTCTGGTGTGCCAAGCGAATCCCCAAGTGCCTATGAGAAGAGCTTGGTACACAAGATCTTTGGCGGTCGCCTTCGAAGCCAGGTCAAGTGTATGCAGTGCTCCTATTGCTCCAACAAGTTTGACCCATTCTTAGATTTAAGTCTTGAAATATTCAACGCAGATTCCTTGCAAAAAGCACTTGGAAACTTTACTGCAGCAGAACAGTTAGATGGAGGGGAAAGGCAATATCAGTGCCAACGATGCAACCAGAAAGTTCGGGCTCTCAAACAAATGACTGTTCAAAAGCCTCCTTATGTACTTACCATCCATTTGAAACGATTTCGTGCACATGATCCTGGGCGAAAAATTGACAGACATGTAAAATTTGGCCCCACATTGGATTTAAAGCCCTTTGTTAGTGGTTCCTATGAAGGAGATTTGAAATATACACTTTATGGGGTTCTTGTTCATTGTGGGGCTACCACCTATTCTGGCCATTATTATTGCTATGTTCGCACATCAAGTGGCATGTGGTACTCCCTTGACGACAACCAGGTATACCAAGTTAGTGAGAGGACTGTTTTAGAACAGAGGGCTTACATGTTGTTTTATGTCCGCGATAGAAGAAATATAATACCGAGGAAGCCTGTTGAAGTTGCTCGGAAGGAGAATTTCAATGCAAATGGTGCTGGATTAAAAACTCCTTCCACTAACAACCAAGGTTCCAAAGTACCCGTTCAGAATGTTTCTGTTGAGGGCAGATCAAGTGGTCCATCCTCATCTATTGTTGTGACACAAAAGTCAACTATAGTTCCACCTATGGTGCCCCTTTTGAAGGAAGCATCAGTTAATTGCCAGACAATGACAGAAAATATGGTGCCTATGAAGGAACCTGCTTCAGAGTTGTCTCCATCATTATCTCCCTTTCCAAGCAGCAATGGTGGTGCTTCTGAGTCTAAAAATGCGGCAGAAAATACAGTGCCTATGAAGGAACCTGTTTCAGAATCTTCATCATCAGTACCAAAGGATTCCTTGAAAGGGATTTCCATTCCAATTCCTAAATTGGGAAAAGATATGCTACCATCATCTCCAACCAGCAATGGTGGCACTTCGGGTCCTGAAAATGCCACAGCTGCAAAAGCTGATGCCAATAAAACTGAACTTAATGAGAGAGGAAGGTCAATTGAAAATGGTGTTTCCAGTGTCATATCACCCAATGTGAAGGACCCTGAAAGCCTTGAGGCTAAACCCATACAAGATGAGACCCCTTATAATAATAATGTAGTTTCCTCGAAGGGAGACTTGAGTATGTCATCCGGAGTTCCTAGCAGTACGAAAAAGGAGGGAATTCACACTGTCAGATCATCAGATGAACCAAGCTCTAAAATTAGTCAGGTTGGAAGTTTTACCAGTGGAAGTGCTGCTTGTAATGTTTTGGGTGAGAAGATTAGTGATTCTGGCCAAAAAGTGGTAATTGACGAGTCAGCAAATTTGCCAGGCTCATCAATTGTGGCAAATGGATCGTTGCTTGTAAAATCTCCTGATTGCAAACGCCATAGAAAATTGAAAAAGAAGCAGCTGAAGTCTAAGGCTGCATGCATGCAGTTACGACCTAGCTTACTTTCCCGGGTGGTCTTAAGTGTACAGAAGAAAAAGAAACACAAAAGGAGTAAGCACCCCTCTTCAGATACCAAGAGCCTTCTTAAGGAACATTTTATGGACAATTCTTGCTTATATGGCATGGGGCCGTCTACATCTGAGCAAACCCGGTCAATTCCCTTGGTAAACCCTTCTAAGCGAAATTCTAAGAGAAAGGGGACCAAATCTGGCTTAAAAAACGATGCAGACAGAACTGATAAAAAGTGGGAAGTGAAATCTCATCGTGATTCTTTAATGGCTGTTCAAAAGGGAGTGTTTAGAGAAACTCTCGGTCAGAATGGTACAGTTCTTGCAACTGATAAAAGACTACAAAATGGTAATGTCTCTAGCTTGTGCTCAAAAGAAAACCTGCGGGAGATGGGGGAAAATGATACCCCACTCAACCATAAAAGAGACAGAACACAAAATGGAGGGATTGGTGTGCTAACCCGTGGCCTTGATGAGACAGTCGTTGCTCGGTGGGACGGAATAGAACTGCCTCGTTCTCATATTGTAGAATCAAGCCGTGCAGAGAGCGTCAGCATCGGTTATATACCCGATGAATGGGATGAAGAATATGATCGCGGAAAGAGAAAGAAGATAAGGCAACCCAAAGAAACGTTTGGCGGACCAAATCCATTCCAAAGGATTGCAACCCAGAGATCAAAGGTGAAAAAGGAAAAGCTAGACAGGTACGGTTCTGGAAATCACCCGCTCAGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH

Protein Analysis

985

Amino Acids

108.15

Weight (kDa)

9.46

Isoelectric Point (pI)

50.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	100 - 401	1.8e-61	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	100 - 383	5.7e-18	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57990
fragaria_vesca	FvH4_1g01010 FvH4_1g01010 FvH4_1g01010
malus_domestica	MD02G1008300.v1.1 MD15G1152500.v1.1
prunus_persica	Prupe.7G262200_v2.0.a1
pyrus_communis	pycom02g00720 pycom15g13710
rosa_chinensis	RchiOBHm_Chr2g0085751
rosa_laevigata	RLG00000015716
rosa_multiflora	Rmu_sc0009759.1_g000004 Rmu_sc0023131.1_g000003 Rmu_sc0033740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155070
rosa_rugosa	Rorug01G0462900 Rorug01G0462900
rosa_samantha	Rh2AG012000 Rh2BG012600 Rh2CG013400 Rh2DG014400
rosa_wichuraiana	Rw2G001070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1863
AasI	GACNNNNNNGTC	1 cut(s)	1390
Acc36I	ACCTGC	2 cut(s)	1492, 2607
AccB1I	GGYRCC	4 cut(s)	296, 490, 1414, 1468
AccB7I	CCANNNNNTGG	1 cut(s)	2059
AccBSI	CCGCTC	1 cut(s)	2944
AccI	GTMKAC	2 cut(s)	1149, 2342
AccII	CGCG	2 cut(s)	1209, 2814
AccIII	TCCGGA	3 cut(s)	259, 285, 1907
AclI	AACGTT	1 cut(s)	2849
AclWI	GGATC	5 cut(s)	45, 251, 507, 938, 2119
AcoI	YGGCCR	3 cut(s)	45, 1084, 2050
AcsI	RAATTY	7 cut(s)	403, 483, 974, 1261, 1938, 2080, 2392
AcuI	CTGAAG	4 cut(s)	59, 1473, 2192, 2259
AfaI	GTAC	9 cut(s)	660, 909, 1127, 1323, 1611, 1924, 2235, 2533, 2924
AflII	CTTAAG	2 cut(s)	2227, 2294
AflIII	ACRYGT	2 cut(s)	967, 1191
AjnI	CCWGG	4 cut(s)	690, 946, 1143, 2086
AloI	GAACNNNNNNTCC	2 cut(s)	236, 268
Alw21I	GWGCWC	5 cut(s)	12, 267, 713, 940, 2588
Alw26I	GTCTC	7 cut(s)	146, 1503, 1847, 1884, 2578, 2637, 2687
Alw44I	GTGCAC	1 cut(s)	936
AlwI	GGATC	5 cut(s)	45, 251, 507, 938, 2119
AlwNI	CAGNNNCTG	1 cut(s)	352
Ama87I	CYCGRG	2 cut(s)	149, 2217
Aor13HI	TCCGGA	3 cut(s)	259, 285, 1907
AoxI	GGCC	6 cut(s)	45, 979, 1084, 2050, 2336, 2683
ApaLI	GTGCAC	1 cut(s)	936
ApeKI	GCWGC	9 cut(s)	171, 183, 374, 806, 1524, 1724, 2012, 2167, 2183
ApoI	RAATTY	7 cut(s)	403, 483, 974, 1261, 1938, 2080, 2392
Asp700I	GAANNNNTTC	2 cut(s)	110, 251
AspS9I	GGNCC	8 cut(s)	231, 980, 1361, 1706, 1816, 2336, 2409, 2858
AsuC2I	CCSGG	3 cut(s)	2218, 2219, 2359
AsuHPI	GGTGA	6 cut(s)	149, 271, 1794, 2042, 2911, 2930
AsuII	TTCGAA	1 cut(s)	685
AvaI	CYCGRG	2 cut(s)	149, 2217
AvaII	GGWCC	6 cut(s)	231, 1361, 1706, 1816, 2409, 2858
BaeGI	GKGCMC	2 cut(s)	940, 1419
BalI	TGGCCA	2 cut(s)	1086, 2052
BanI	GGYRCC	4 cut(s)	296, 490, 1414, 1468
BanII	GRGCYC	3 cut(s)	12, 267, 874
Bbv12I	GWGCWC	5 cut(s)	12, 267, 713, 940, 2588
BbvI	GCAGC	9 cut(s)	183, 195, 361, 818, 1536, 1711, 1999, 2170, 2179
BccI	CCATC	8 cut(s)	20, 221, 815, 923, 1372, 1510, 1681, 2602
BceAI	ACGGC	2 cut(s)	2323, 2739
BcgI	CGANNNNNNTGC	4 cut(s)	390, 424, 2094, 2128
BciT130I	CCWGG	4 cut(s)	692, 948, 1145, 2088
BcnI	CCSGG	3 cut(s)	2218, 2219, 2359
BcoDI	GTCTC	7 cut(s)	146, 1503, 1847, 1884, 2578, 2637, 2687
BfaI	CTAG	7 cut(s)	189, 503, 546, 1917, 2205, 2577, 2915
BfmI	CTRYAG	2 cut(s)	804, 1398
BfrI	CTTAAG	2 cut(s)	2227, 2294
BfuAI	ACCTGC	2 cut(s)	1492, 2607
BglII	AGATCT	1 cut(s)	666
BlpI	GCTNAGC	1 cut(s)	266
Bme1390I	CCNGG	7 cut(s)	692, 948, 1145, 2088, 2218, 2219, 2359
Bme18I	GGWCC	6 cut(s)	231, 1361, 1706, 1816, 2409, 2858
BmeT110I	CYCGRG	2 cut(s)	149, 2217
BmgT120I	GGNCC	8 cut(s)	231, 980, 1361, 1706, 1816, 2336, 2409, 2858
BmrFI	CCNGG	7 cut(s)	692, 948, 1145, 2088, 2218, 2219, 2359
BmrI	ACTGGG	2 cut(s)	69, 486
BmsI	GCATC	8 cut(s)	285, 406, 547, 842, 1442, 1726, 2422, 2781
BmuI	ACTGGG	2 cut(s)	69, 486
BplI	GAGNNNNNCTC	2 cut(s)	2502, 2534
BpmI	CTGGAG	1 cut(s)	605
Bpu10I	CCTNAGC	1 cut(s)	2945
Bpu1102I	GCTNAGC	1 cut(s)	266
Bpu14I	TTCGAA	1 cut(s)	685
BpuEI	CTTGAG	2 cut(s)	1850, 1915
BpuMI	CCSGG	3 cut(s)	2218, 2219, 2359
BsaI	GGTCTC	2 cut(s)	146, 1847
BsaJI	CCNNGG	6 cut(s)	947, 1229, 1309, 2217, 2370, 2680
BsaWI	WCCGGW	3 cut(s)	259, 285, 1907
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 280, 310
Bse118I	RCCGGY	1 cut(s)	299
Bse1I	ACTGG	5 cut(s)	64, 133, 481, 1791, 2001
Bse3DI	GCAATG	2 cut(s)	1534, 1696
BseAI	TCCGGA	3 cut(s)	259, 285, 1907
BseBI	CCWGG	4 cut(s)	692, 948, 1145, 2088
BseDI	CCNNGG	6 cut(s)	947, 1229, 1309, 2217, 2370, 2680
BseGI	GGATG	7 cut(s)	31, 232, 562, 915, 1364, 1904, 2803
BseMI	GCAATG	2 cut(s)	1534, 1696
BseMII	CTCAG	4 cut(s)	280, 354, 1533, 2340
BseNI	ACTGG	5 cut(s)	64, 133, 481, 1791, 2001
BseSI	GKGCMC	2 cut(s)	940, 1419
BseXI	GCAGC	9 cut(s)	183, 195, 361, 818, 1536, 1711, 1999, 2170, 2179
BseYI	CCCAGC	1 cut(s)	419
BsgI	GTGCAG	1 cut(s)	2778
Bsh1236I	CGCG	2 cut(s)	1209, 2814
Bsh1285I	CGRYCG	1 cut(s)	679
BshFI	GGCC	6 cut(s)	47, 981, 1086, 2052, 2338, 2685
BshNI	GGYRCC	4 cut(s)	296, 490, 1414, 1468
BsiEI	CGRYCG	1 cut(s)	679
BsiHKAI	GWGCWC	5 cut(s)	12, 267, 713, 940, 2588
BsiHKCI	CYCGRG	2 cut(s)	149, 2217
BsiSI	CCGG	7 cut(s)	145, 260, 286, 300, 1908, 2218, 2359
BslFI	GGGAC	2 cut(s)	2422, 2726
BsmAI	GTCTC	7 cut(s)	146, 1503, 1847, 1884, 2578, 2637, 2687
BsmFI	GGGAC	2 cut(s)	2422, 2726
BsnI	GGCC	6 cut(s)	47, 981, 1086, 2052, 2338, 2685
Bso31I	GGTCTC	2 cut(s)	146, 1847
BsoBI	CYCGRG	2 cut(s)	149, 2217
Bsp119I	TTCGAA	1 cut(s)	685
Bsp1286I	GDGCHC	7 cut(s)	12, 267, 713, 874, 940, 1419, 2588
Bsp13I	TCCGGA	3 cut(s)	259, 285, 1907
Bsp1407I	TGTACA	1 cut(s)	2233
Bsp1720I	GCTNAGC	1 cut(s)	266
BspANI	GGCC	6 cut(s)	47, 981, 1086, 2052, 2338, 2685
BspCNI	CTCAG	4 cut(s)	279, 355, 1534, 2341
BspEI	TCCGGA	3 cut(s)	259, 285, 1907
BspFNI	CGCG	2 cut(s)	1209, 2814
BspMAI	CTGCAG	1 cut(s)	808
BspMI	ACCTGC	2 cut(s)	1492, 2607
BspPI	GGATC	5 cut(s)	45, 251, 507, 938, 2119
BspQI	GCTCTTC	1 cut(s)	644
BspT104I	TTCGAA	1 cut(s)	685
BspT107I	GGYRCC	4 cut(s)	296, 490, 1414, 1468
BspTI	CTTAAG	2 cut(s)	2227, 2294
BspTNI	GGTCTC	2 cut(s)	146, 1847
BsrBI	CCGCTC	1 cut(s)	2944
BsrDI	GCAATG	2 cut(s)	1534, 1696
BsrFI	RCCGGY	1 cut(s)	299
BsrGI	TGTACA	1 cut(s)	2233
BsrI	ACTGG	5 cut(s)	64, 133, 481, 1791, 2001
BssAI	RCCGGY	1 cut(s)	299
BssECI	CCNNGG	6 cut(s)	947, 1229, 1309, 2217, 2370, 2680
BssNAI	GTATAC	1 cut(s)	1150
BssT1I	CCWWGG	2 cut(s)	1309, 2370
Bst1107I	GTATAC	1 cut(s)	1150
Bst2UI	CCWGG	4 cut(s)	692, 948, 1145, 2088
Bst4CI	ACNGT	9 cut(s)	612, 816, 889, 1171, 1570, 1948, 2536, 2698, 2927
Bst6I	CTCTTC	2 cut(s)	644, 2278
BstAFI	CTTAAG	2 cut(s)	2227, 2294
BstAPI	GCANNNNNTGC	1 cut(s)	1277
BstAUI	TGTACA	1 cut(s)	2233
BstBI	TTCGAA	1 cut(s)	685
BstC8I	GCNNGC	2 cut(s)	2188, 2192
BstDSI	CCRYGG	1 cut(s)	2680
BstENI	CCTNNNNNAGG	2 cut(s)	1424, 2294
BstF5I	GGATG	7 cut(s)	31, 232, 562, 915, 1364, 1904, 2803
BstFNI	CGCG	2 cut(s)	1209, 2814
BstMAI	GTCTC	7 cut(s)	146, 1503, 1847, 1884, 2578, 2637, 2687
BstMCI	CGRYCG	1 cut(s)	679
BstNI	CCWGG	4 cut(s)	692, 948, 1145, 2088
BstNSI	RCATGY	6 cut(s)	455, 971, 1123, 1195, 2190, 2194
BstSCI	CCNGG	7 cut(s)	690, 946, 1143, 2086, 2216, 2217, 2357
BstSFI	CTRYAG	2 cut(s)	804, 1398
BstSLI	GKGCMC	2 cut(s)	940, 1419
BstUI	CGCG	2 cut(s)	1209, 2814
BstV1I	GCAGC	9 cut(s)	183, 195, 361, 818, 1536, 1711, 1999, 2170, 2179
BstX2I	RGATCY	3 cut(s)	37, 499, 666
BstXI	CCANNNNNNTGG	1 cut(s)	419
BstYI	RGATCY	3 cut(s)	37, 499, 666
BstZ17I	GTATAC	1 cut(s)	1150
BsuRI	GGCC	6 cut(s)	47, 981, 1086, 2052, 2338, 2685
BtgI	CCRYGG	1 cut(s)	2680
BtsCI	GGATG	7 cut(s)	31, 232, 562, 915, 1364, 1904, 2803
BtsI	GCAGTG	2 cut(s)	353, 713
BveI	ACCTGC	2 cut(s)	1492, 2607
Cac8I	GCNNGC	2 cut(s)	2188, 2192
CaiI	CAGNNNCTG	1 cut(s)	352
Cfr10I	RCCGGY	1 cut(s)	299
Cfr13I	GGNCC	8 cut(s)	231, 980, 1361, 1706, 1816, 2336, 2409, 2858
Cfr9I	CCCGGG	1 cut(s)	2217
CseI	GACGC	1 cut(s)	2755
CsiI	ACCWGGT	1 cut(s)	1143
Csp6I	GTAC	9 cut(s)	659, 908, 1126, 1322, 1610, 1923, 2234, 2532, 2923
CviQI	GTAC	9 cut(s)	659, 908, 1126, 1322, 1610, 1923, 2234, 2532, 2923
DraI	TTTAAA	1 cut(s)	996
DrdI	GACNNNNNNGTC	1 cut(s)	1390
DseDI	GACNNNNNNGTC	1 cut(s)	1390
EaeI	YGGCCR	3 cut(s)	45, 1084, 2050
Eam1104I	CTCTTC	2 cut(s)	644, 2278
EarI	CTCTTC	2 cut(s)	644, 2278
EciI	GGCGGA	3 cut(s)	20, 192, 2871
Ecl136II	GAGCTC	2 cut(s)	10, 265
Eco130I	CCWWGG	2 cut(s)	1309, 2370
Eco24I	GRGCYC	3 cut(s)	12, 267, 874
Eco31I	GGTCTC	2 cut(s)	146, 1847
Eco47I	GGWCC	6 cut(s)	231, 1361, 1706, 1816, 2409, 2858
Eco53kI	GAGCTC	2 cut(s)	10, 265
Eco57I	CTGAAG	4 cut(s)	59, 1473, 2192, 2259
Eco88I	CYCGRG	2 cut(s)	149, 2217
EcoICRI	GAGCTC	2 cut(s)	10, 265
EcoNI	CCTNNNNNAGG	2 cut(s)	1424, 2294
EcoO109I	RGGNCCY	2 cut(s)	1706, 1816
EcoRI	GAATTC	1 cut(s)	1938
EcoRII	CCWGG	4 cut(s)	690, 946, 1143, 2086
EcoT14I	CCWWGG	2 cut(s)	1309, 2370
EcoT22I	ATGCAT	3 cut(s)	524, 597, 2192
EcoT38I	GRGCYC	3 cut(s)	12, 267, 874
ErhI	CCWWGG	2 cut(s)	1309, 2370
FalI	AAGNNNNNCTT	6 cut(s)	1607, 1639, 1877, 1909, 1999, 2031
FaqI	GGGAC	2 cut(s)	2422, 2726
FauI	CCCGC	2 cut(s)	2595, 2949
FblI	GTMKAC	2 cut(s)	1149, 2342
FokI	GGATG	7 cut(s)	38, 239, 569, 902, 1351, 1891, 2810
FriOI	GRGCYC	3 cut(s)	12, 267, 874
FspBI	CTAG	7 cut(s)	189, 503, 546, 1917, 2205, 2577, 2915
GsaI	CCCAGC	1 cut(s)	423
GsuI	CTGGAG	1 cut(s)	605
HaeIII	GGCC	6 cut(s)	47, 981, 1086, 2052, 2338, 2685
HapII	CCGG	7 cut(s)	145, 260, 286, 300, 1908, 2218, 2359
HgaI	GACGC	1 cut(s)	2755
HincII	GTYRAC	1 cut(s)	1395
HindII	GTYRAC	1 cut(s)	1395
HpaII	CCGG	7 cut(s)	145, 260, 286, 300, 1908, 2218, 2359
HphI	GGTGA	6 cut(s)	149, 271, 1794, 2042, 2911, 2930
Hpy166II	GTNNAC	9 cut(s)	577, 608, 661, 938, 1150, 1395, 2234, 2343, 2377
Hpy8I	GTNNAC	9 cut(s)	577, 608, 661, 938, 1150, 1395, 2234, 2343, 2377
HpyCH4III	ACNGT	9 cut(s)	612, 816, 889, 1171, 1570, 1948, 2536, 2698, 2927
HpyCH4IV	ACGT	1 cut(s)	2849
HpySE526I	ACGT	1 cut(s)	2849
Kpn2I	TCCGGA	3 cut(s)	259, 285, 1907
LguI	GCTCTTC	1 cut(s)	644
LmnI	GCTCC	5 cut(s)	7, 15, 262, 716, 725
Lsp1109I	GCAGC	9 cut(s)	183, 195, 361, 818, 1536, 1711, 1999, 2170, 2179
LweI	GCATC	8 cut(s)	285, 406, 547, 842, 1442, 1726, 2422, 2781
MabI	ACCWGGT	1 cut(s)	1143
MaeI	CTAG	7 cut(s)	189, 503, 546, 1917, 2205, 2577, 2915
MaeII	ACGT	1 cut(s)	2849
MaeIII	GTNAC	3 cut(s)	137, 1381, 2196
MbiI	CCGCTC	1 cut(s)	2944
MboII	GAAGA	8 cut(s)	661, 1227, 1590, 2047, 2251, 2265, 2813, 2839
MfeI	CAATTG	2 cut(s)	1773, 2097
MflI	RGATCY	3 cut(s)	37, 499, 666
MhlI	GDGCHC	7 cut(s)	12, 267, 713, 874, 940, 1419, 2588
MlsI	TGGCCA	2 cut(s)	1086, 2052
MluNI	TGGCCA	2 cut(s)	1086, 2052
MlyI	GAGTC	3 cut(s)	596, 1553, 2081
MmeI	TCCRAC	3 cut(s)	747, 1706, 1969
Mox20I	TGGCCA	2 cut(s)	1086, 2052
Mph1103I	ATGCAT	3 cut(s)	524, 597, 2192
MroI	TCCGGA	3 cut(s)	259, 285, 1907
MroXI	GAANNNNTTC	2 cut(s)	110, 251
MscI	TGGCCA	2 cut(s)	1086, 2052
Msp20I	TGGCCA	2 cut(s)	1086, 2052
MspA1I	CMGCKG	3 cut(s)	174, 1724, 2170
MspCI	CTTAAG	2 cut(s)	2227, 2294
MspI	CCGG	7 cut(s)	145, 260, 286, 300, 1908, 2218, 2359
MspR9I	CCNGG	7 cut(s)	692, 948, 1145, 2088, 2218, 2219, 2359
MunI	CAATTG	2 cut(s)	1773, 2097
MvaI	CCWGG	4 cut(s)	692, 948, 1145, 2088
MvnI	CGCG	2 cut(s)	1209, 2814
NciI	CCSGG	3 cut(s)	2218, 2219, 2359
NmuCI	GTSAC	2 cut(s)	137, 1381
NsiI	ATGCAT	3 cut(s)	524, 597, 2192
NspI	RCATGY	6 cut(s)	455, 971, 1123, 1195, 2190, 2194
NspV	TTCGAA	1 cut(s)	685
PaeI	GCATGC	2 cut(s)	2190, 2194
PaeR7I	CTCGAG	1 cut(s)	149
PciI	ACATGT	2 cut(s)	967, 1191
PciSI	GCTCTTC	1 cut(s)	644
PdmI	GAANNNNTTC	2 cut(s)	110, 251
PfeI	GAWTC	9 cut(s)	50, 160, 629, 773, 1595, 1619, 2045, 2477, 2747
PflMI	CCANNNNNTGG	1 cut(s)	2059
PleI	GAGTC	3 cut(s)	595, 1552, 2080
PpsI	GAGTC	3 cut(s)	595, 1552, 2080
PpuMI	RGGWCCY	2 cut(s)	1706, 1816
PscI	ACATGT	2 cut(s)	967, 1191
PsiI	TTATAA	1 cut(s)	1863
Psp124BI	GAGCTC	2 cut(s)	12, 267
Psp1406I	AACGTT	1 cut(s)	2849
Psp5II	RGGWCCY	2 cut(s)	1706, 1816
Psp6I	CCWGG	4 cut(s)	690, 946, 1143, 2086
PspFI	CCCAGC	1 cut(s)	419
PspGI	CCWGG	4 cut(s)	690, 946, 1143, 2086
PspPI	GGNCC	8 cut(s)	231, 980, 1361, 1706, 1816, 2336, 2409, 2858
PspPPI	RGGWCCY	2 cut(s)	1706, 1816
PstI	CTGCAG	1 cut(s)	808
PstNI	CAGNNNCTG	1 cut(s)	352
PsuI	RGATCY	3 cut(s)	37, 499, 666
PvuII	CAGCTG	2 cut(s)	1724, 2170
RsaI	GTAC	9 cut(s)	660, 909, 1127, 1323, 1611, 1924, 2235, 2533, 2924
RsaNI	GTAC	9 cut(s)	659, 908, 1126, 1322, 1610, 1923, 2234, 2532, 2923
SacI	GAGCTC	2 cut(s)	12, 267
SapI	GCTCTTC	1 cut(s)	644
Sau96I	GGNCC	8 cut(s)	231, 980, 1361, 1706, 1816, 2336, 2409, 2858
SchI	GAGTC	3 cut(s)	596, 1553, 2081
ScrFI	CCNGG	7 cut(s)	692, 948, 1145, 2088, 2218, 2219, 2359
SduI	GDGCHC	7 cut(s)	12, 267, 713, 874, 940, 1419, 2588
SexAI	ACCWGGT	1 cut(s)	1143
SfaNI	GCATC	8 cut(s)	285, 406, 547, 842, 1442, 1726, 2422, 2781
SfcI	CTRYAG	2 cut(s)	804, 1398
Sfr274I	CTCGAG	1 cut(s)	149
SfuI	TTCGAA	1 cut(s)	685
SinI	GGWCC	6 cut(s)	231, 1361, 1706, 1816, 2409, 2858
SlaI	CTCGAG	1 cut(s)	149
SmaI	CCCGGG	1 cut(s)	2219
SmlI	CTYRAG	5 cut(s)	149, 1829, 1894, 2227, 2294
SmoI	CTYRAG	5 cut(s)	149, 1829, 1894, 2227, 2294
SphI	GCATGC	2 cut(s)	2190, 2194
SspI	AATATT	1 cut(s)	762
SspMI	CTAG	7 cut(s)	189, 503, 546, 1917, 2205, 2577, 2915
SstI	GAGCTC	2 cut(s)	12, 267
StyD4I	CCNGG	7 cut(s)	690, 946, 1143, 2086, 2216, 2217, 2357
StyI	CCWWGG	2 cut(s)	1309, 2370
TaaI	ACNGT	9 cut(s)	612, 816, 889, 1171, 1570, 1948, 2536, 2698, 2927
TaiI	ACGT	1 cut(s)	2852
TaqI	TCGA	6 cut(s)	150, 255, 400, 529, 685, 1883
TaqII	GACCGA	1 cut(s)	2510
TatI	WGTACW	2 cut(s)	907, 2233
TauI	GCSGC	3 cut(s)	135, 177, 1559
TfiI	GAWTC	9 cut(s)	50, 160, 629, 773, 1595, 1619, 2045, 2477, 2747
TseFI	GTSAC	2 cut(s)	137, 1381
TseI	GCWGC	9 cut(s)	171, 183, 374, 806, 1524, 1724, 2012, 2167, 2183
Tsp45I	GTSAC	2 cut(s)	137, 1381
TspGWI	ACGGA	2 cut(s)	180, 2730
TspMI	CCCGGG	1 cut(s)	2217
Van91I	CCANNNNNTGG	1 cut(s)	2059
Vha464I	CTTAAG	2 cut(s)	2227, 2294
VneI	GTGCAC	1 cut(s)	936
VpaK11BI	GGWCC	6 cut(s)	231, 1361, 1706, 1816, 2409, 2858
XagI	CCTNNNNNAGG	2 cut(s)	1424, 2294
XapI	RAATTY	7 cut(s)	403, 483, 974, 1261, 1938, 2080, 2392
XbaI	TCTAGA	1 cut(s)	545
XceI	RCATGY	6 cut(s)	455, 971, 1123, 1195, 2190, 2194
XhoI	CTCGAG	1 cut(s)	149
XmaI	CCCGGG	1 cut(s)	2217
XmiI	GTMKAC	2 cut(s)	1149, 2342
XmnI	GAANNNNTTC	2 cut(s)	110, 251
XspI	CTAG	7 cut(s)	189, 503, 546, 1917, 2205, 2577, 2915
Zsp2I	ATGCAT	3 cut(s)	524, 597, 2192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.