Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009301

Physical Location & Seq

Genomic Coordinates Forward (+)

91480301 .. 91486011

Gene Structure 5711 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00155070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2796 bp

ATGGCCGACGTGTTCAATCCGGCAATCCGATCGGGTCAGAGTTCGGATCCGCAGCTCAAATCGGCGGAGGAGGCCTCGCCGTTTCCGAGGATCGAGTTTCATCTGGCGCGGAAGCCATTCAAGGGTTTTAACAATGACGGCAGTGACTTCCGGCCCGAGACGCTTAATCCGGGGAGCGGTGGCAGCTCGGATTCGCGTAAACCCGGCGGCCCGGGAGGTCAGGGTCAGTCGGGTTCGGGTTCGGGAGCGAAGAAGAGTGACGGGTCGGAGTTTTTGGAGAACGGGTGGGATCCGGAGCTCAGCTTTCCCATCACATTTCGGAGAATTGGCGCTGGTTTGATAAATATGGGGAATACTTGTTATCTCAATTCAGTGTTGCAGTGCCTGACATATACTGAGCCTTTAGCTGCATACCTGCAAAGTGGCAGGCATCGGAATTCTTGTCATATAAAAAATGGGTTTTGTGCTTTATGTGCTATCCAAGAACACGTCAGTCTTGCTCTGCAATCAACTAAGAGCATTTTGCCAAAGGCTCTTGTCTTCAATCTACAACGCATATCGCGGAACTTTAGGAAGTCCAGACAGGAAGATGCACATGAATACATGGTAAACTTGCTGGAATCCATGCATAAATGTTGTTTACCATCTGGAGTGCCAAGTGAATCCCCTAGCGCCTATGAGAAGAGTTTGGTACACAAGATCTTTGGTGGTCGCCTTCGAAGTCGGGTCAAATGTTTGCAGTGCTCCTATTGCTCTGACAAGTTAGATCCATTCTTAGATTTAAGTCTTGAAATAGCCAAGGCAAACTCTTTGAATAAAGCACTTGTGAACTTTACTGCTGAAGAACAGTTGGATGGTGGGGAAAGGCAATATCAGTGCCAAAGATGCAATCAGAAAGTTCGAGCTATGAAACAGATGACCATTCACAAGCCGCCTAGTGTGCTGTCCATCCATTTAAAGCGGTTTCGTGCGTATGACCCTGGACAAAAAATTAACAAAGAAGTTCAGTTTGGTCCTACGTTGGACTTGAGACCTTTTGTCAGTGGAGCATATCCGGAAGGAGATTTGAAATATACTCTTTATGGGGTTCTAGTTCATTGTGGGGCTAGCACCTATTCCGGCCATTATTATTGCTTTGTTCGAACATCAACTGGCATATGGCACTCTCTGGATGACAACAATGTAAGGCAAGTTCGTGAGATGACTGTTTTGCAGCAAAAGGCTTACATGTTGTTCTATGTACGTGATAGAAGAAATATTATTCCAAGGAAGCCTGTTGATGTTGCTCAAAAGGAGTATTTCAAACCTAATGGTACTGTAAATAAAATTACTCCTACTGACAACCATTTGTCGAAAGAACCTATTCGAAAAATTGCAGTTGCGGGCAGATCCAATGATCTAGCCTCTTCTGTTGCACAAAAAGATGCATCAGCTATTGTCCCAAGGGTGTCCCATTTGAAGGAAGCATCAGTTAATCAAAATAATGGCCACATAGTGGCACAAAATATGGTGCGTAAGAAGGAAGCCATTTCAGAATCGTCTTCGAAAGTGTCATTATCAGAGGGCTCATTGGGAGGATTAGGAGAAAATATGCCACCATCTACTCTGCTTCCAAGCCGCAAAACTGGCACTTCTGATTCTGCAAGTGCTGTGGCTTCAACAACTGGTGCCAAACTAAATGACTGCAATGAGAAAGCAAGTTCAAATGATAATGATAGGGTTTCAATTATGATATCACCTACTGTGAAGGACCCTGAAATCTTAAAGGCATCTAAGCCTGTTCAAGATGAGATCCCTCAGAATAATCATGCTTCTGGTGCTGGGAATTTGAGTGTGGCTTCATCCAGAATTCCTAGTGATTTAACATTGGAAAAGCCATCTGATCCACCAAGCTCTAGAATCAGTCAGGCTGGAAGTTTTACCAATGAACGTGAGAAGGCTAGTGATTGTGGCCAGACAGTGGTAGTTTCTGATTCTGCAAAATTGTCTGGCTCATCAGTTGTGACAAATAGATCATTAGATTTGGAAGCTCCTGACCGCAAGCACCCTAAAGGATTGAAAAAGAAGCACATAAAGTGTAAGAAGGTAGGCATGCATTTCCGGCCATCCTTGCTTTCTAGGGCAGTCTTAAGTGTGCAGAAGAAGAAGAAGCACAAAAGGAGTAAACACCCAATTTCGGATTGTAAGAGCCATAGTAAGAAATACTTGGTGGAAAATTATTGTTCTTCATCTGATCAAGGACCATCTACATCTGAGAAAACCCAGACAGATTCCTTGGTTTCTGTCTGTTCGAAGAGAAAGAGAAACAAATCTGGCTTGAAAACGGATGAAGATGGAACTTCTAATAAGTGCTCACTGAATCCCCATGGTGATTCTTCGATGGATATTAAAGATGTAGAGTTTAGAGAGAGCCTCATTCAGAATGGAACAGTGCTTGCAAGTGATAAACAACAACAAAATGGCTTTGGCTCTTCCTCTGTTACATTGGAAAACAAAAGGGAGTCGACAGGAACTGATACTCCACACAATTGTAAAAGAGATGCAATGCAAAATGGATGGAAGGGTGTGCTGATTCAAGGTCCAGTGGAGACAATTGTTGCACCTTGGGATGGAATAGAATTGTCTCAGTCTCATGTTGTAGAATCAAGCCATGCACAGAGTATCACCATCGGTTATGTAGGAGATGATTGGGATGAAGAATATGATCGGGGAAAGAGAAAGAAGGTAAGGCAAGCAAAACTCACCCCTGGTGGGCAAATCCTTTCCAAATGCTTGCAAACAAGAAAGCACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH

Protein Analysis

931

Amino Acids

102.17

Weight (kDa)

9.32

Isoelectric Point (pI)

49.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	111 - 413	1.1e-61	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	111 - 394	1.1e-15	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57990
fragaria_vesca	FvH4_1g01010 FvH4_1g01010 FvH4_1g01010
malus_domestica	MD02G1008300.v1.1 MD15G1152500.v1.1
prunus_persica	Prupe.7G262200_v2.0.a1
pyrus_communis	pycom02g00720 pycom15g13710
rosa_chinensis	RchiOBHm_Chr2g0085751
rosa_laevigata	RLG00000015716
rosa_multiflora	Rmu_sc0009759.1_g000004 Rmu_sc0023131.1_g000003 Rmu_sc0033740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155070
rosa_rugosa	Rorug01G0462900 Rorug01G0462900
rosa_samantha	Rh2AG012000 Rh2BG012600 Rh2CG013400 Rh2DG014400
rosa_wichuraiana	Rw2G001070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	423
AccB1I	GGYRCC	1 cut(s)	1667
AccB7I	CCANNNNNTGG	2 cut(s)	1495, 1960
AccBSI	CCGCTC	1 cut(s)	177
AccI	GTMKAC	1 cut(s)	2504
AccII	CGCG	3 cut(s)	109, 196, 562
AccIII	TCCGGA	2 cut(s)	292, 1054
AclWI	GGATC	9 cut(s)	41, 54, 98, 284, 297, 761, 1383, 1786, 1877
AcoI	YGGCCR	5 cut(s)	3, 1120, 1486, 1951, 2102
AcsI	RAATTY	3 cut(s)	436, 1825, 1848
AcuI	CTGAAG	1 cut(s)	861
AdeI	CACNNNGTG	1 cut(s)	1495
AfaI	GTAC	3 cut(s)	693, 1242, 1315
AfiI	CCNNNNNNNGG	8 cut(s)	122, 176, 1459, 1495, 1960, 2176, 2609, 2751
AflII	CTTAAG	1 cut(s)	2128
AflIII	ACRYGT	3 cut(s)	9, 487, 1227
AhdI	GACNNNNNGTC	1 cut(s)	1037
AjiI	CACGTC	2 cut(s)	10, 490
AjnI	CCWGG	2 cut(s)	979, 2746
AjuI	GAANNNNNNNTTGG	2 cut(s)	993, 1025
AloI	GAACNNNNNNTCC	2 cut(s)	2503, 2535
AluBI	AGCT	9 cut(s)	55, 186, 298, 303, 407, 905, 1433, 1893, 2030
AluI	AGCT	9 cut(s)	55, 186, 298, 303, 407, 905, 1433, 1893, 2030
Alw21I	GWGCWC	3 cut(s)	300, 746, 2354
Alw26I	GTCTC	5 cut(s)	152, 1024, 2582, 2628, 2634
AlwI	GGATC	9 cut(s)	41, 54, 98, 284, 297, 761, 1383, 1786, 1877
AlwNI	CAGNNNCTG	2 cut(s)	385, 2513
Ama87I	CYCGRG	2 cut(s)	155, 211
Aor13HI	TCCGGA	2 cut(s)	292, 1054
AoxI	GGCC	8 cut(s)	3, 72, 152, 208, 1120, 1486, 1951, 2102
ApeKI	GCWGC	4 cut(s)	52, 183, 407, 1213
ApoI	RAATTY	3 cut(s)	436, 1825, 1848
Asp700I	GAANNNNTTC	2 cut(s)	116, 1362
AspLEI	GCGC	3 cut(s)	109, 332, 674
AspS9I	GGNCC	6 cut(s)	153, 209, 1013, 1750, 2240, 2579
AsuC2I	CCSGG	4 cut(s)	171, 204, 212, 213
AsuHPI	GGTGA	4 cut(s)	1728, 2381, 2656, 2734
AsuII	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
AsuNHI	GCTAGC	1 cut(s)	1106
AvaI	CYCGRG	2 cut(s)	155, 211
AvaII	GGWCC	4 cut(s)	1013, 1750, 2240, 2579
BalI	TGGCCA	2 cut(s)	1488, 1953
BamHI	GGATCC	2 cut(s)	46, 289
BanI	GGYRCC	1 cut(s)	1667
BanII	GRGCYC	2 cut(s)	300, 1568
BbsI	GAAGAC	2 cut(s)	532, 1533
Bbv12I	GWGCWC	3 cut(s)	300, 746, 2354
BbvI	GCAGC	4 cut(s)	64, 195, 394, 1225
BceAI	ACGGC	2 cut(s)	64, 154
BcgI	CGANNNNNNTGC	2 cut(s)	12, 46
BciT130I	CCWGG	2 cut(s)	981, 2748
BclI	TGATCA	1 cut(s)	2233
BcnI	CCSGG	4 cut(s)	171, 204, 212, 213
BcoDI	GTCTC	5 cut(s)	152, 1024, 2582, 2628, 2634
BfaI	CTAG	9 cut(s)	669, 936, 1091, 1107, 1400, 1854, 1896, 1941, 2118
BfoI	RGCGCY	2 cut(s)	333, 675
BfrI	CTTAAG	1 cut(s)	2128
BfuAI	ACCTGC	1 cut(s)	423
BglII	AGATCT	1 cut(s)	699
BisI	GCNGC	7 cut(s)	53, 184, 208, 408, 932, 1214, 1618
BlpI	GCTNAGC	1 cut(s)	299
BlsI	GCNGC	7 cut(s)	54, 185, 209, 409, 933, 1215, 1619
Bme1390I	CCNGG	6 cut(s)	171, 204, 212, 213, 981, 2748
Bme18I	GGWCC	4 cut(s)	1013, 1750, 2240, 2579
BmeRI	GACNNNNNGTC	1 cut(s)	1037
BmeT110I	CYCGRG	2 cut(s)	155, 211
BmgBI	CACGTC	2 cut(s)	10, 490
BmgT120I	GGNCC	6 cut(s)	153, 209, 1013, 1750, 2240, 2579
BmiI	GGNNCC	4 cut(s)	48, 291, 1669, 1752
BmrFI	CCNGG	6 cut(s)	171, 204, 212, 213, 981, 2748
BmsI	GCATC	8 cut(s)	439, 580, 875, 1414, 1436, 1475, 1778, 2530
BmtI	GCTAGC	1 cut(s)	1110
BpiI	GAAGAC	2 cut(s)	532, 1533
BplI	GAGNNNNNCTC	4 cut(s)	59, 91, 2397, 2429
BpmI	CTGGAG	1 cut(s)	669
Bpu1102I	GCTNAGC	1 cut(s)	299
Bpu14I	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
BpuEI	CTTGAG	1 cut(s)	1048
BpuMI	CCSGG	4 cut(s)	171, 204, 212, 213
BsaAI	YACGTR	1 cut(s)	1244
BsaI	GGTCTC	1 cut(s)	1024
BsaWI	WCCGGW	2 cut(s)	292, 1054
BsaXI	ACNNNNNCTCC	4 cut(s)	269, 299, 2503, 2533
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 176, 1459, 1495, 1960, 2176, 2609, 2751
Bse1I	ACTGG	4 cut(s)	1156, 1630, 1669, 2582
Bse3DI	GCAATG	2 cut(s)	1693, 2550
BseAI	TCCGGA	2 cut(s)	292, 1054
BseBI	CCWGG	2 cut(s)	981, 2748
BseGI	GGATG	9 cut(s)	859, 948, 1177, 1841, 2105, 2332, 2561, 2614, 2698
BseLI	CCNNNNNNNGG	8 cut(s)	122, 176, 1459, 1495, 1960, 2176, 2609, 2751
BseMI	GCAATG	2 cut(s)	1693, 2550
BseMII	CTCAG	5 cut(s)	313, 387, 1811, 2244, 2639
BseNI	ACTGG	4 cut(s)	1156, 1630, 1669, 2582
BseRI	GAGGAG	1 cut(s)	83
BseXI	GCAGC	4 cut(s)	64, 195, 394, 1225
BseYI	CCCAGC	1 cut(s)	1820
BsgI	GTGCAG	1 cut(s)	2156
Bsh1236I	CGCG	3 cut(s)	109, 196, 562
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	8 cut(s)	5, 74, 154, 210, 1122, 1488, 1953, 2104
BshNI	GGYRCC	1 cut(s)	1667
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	3 cut(s)	300, 746, 2354
BsiHKCI	CYCGRG	2 cut(s)	155, 211
BsiSI	CCGG	9 cut(s)	20, 151, 170, 204, 212, 293, 1055, 1119, 2101
BslFI	GGGAC	2 cut(s)	1424, 1435
BslI	CCNNNNNNNGG	8 cut(s)	122, 176, 1459, 1495, 1960, 2176, 2609, 2751
BsmAI	GTCTC	5 cut(s)	152, 1024, 2582, 2628, 2634
BsmBI	CGTCTC	1 cut(s)	152
BsmFI	GGGAC	2 cut(s)	1424, 1435
BsnI	GGCC	8 cut(s)	5, 74, 154, 210, 1122, 1488, 1953, 2104
Bso31I	GGTCTC	1 cut(s)	1024
BsoBI	CYCGRG	2 cut(s)	155, 211
Bsp119I	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
Bsp1286I	GDGCHC	4 cut(s)	300, 746, 1568, 2354
Bsp13I	TCCGGA	2 cut(s)	292, 1054
Bsp1720I	GCTNAGC	1 cut(s)	299
Bsp19I	CCATGG	1 cut(s)	2365
BspANI	GGCC	8 cut(s)	5, 74, 154, 210, 1122, 1488, 1953, 2104
BspCNI	CTCAG	5 cut(s)	312, 388, 1810, 2245, 2638
BspEI	TCCGGA	2 cut(s)	292, 1054
BspFNI	CGCG	3 cut(s)	109, 196, 562
BspLI	GGNNCC	4 cut(s)	48, 291, 1669, 1752
BspMI	ACCTGC	1 cut(s)	423
BspOI	GCTAGC	1 cut(s)	1110
BspPI	GGATC	9 cut(s)	41, 54, 98, 284, 297, 761, 1383, 1786, 1877
BspQI	GCTCTTC	1 cut(s)	2476
BspT104I	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
BspT107I	GGYRCC	1 cut(s)	1667
BspTI	CTTAAG	1 cut(s)	2128
BspTNI	GGTCTC	1 cut(s)	1024
BsrBI	CCGCTC	1 cut(s)	177
BsrDI	GCAATG	2 cut(s)	1693, 2550
BsrI	ACTGG	4 cut(s)	1156, 1630, 1669, 2582
BssT1I	CCWWGG	6 cut(s)	798, 1265, 1442, 2274, 2365, 2603
Bst2UI	CCWGG	2 cut(s)	981, 2748
Bst4CI	ACNGT	6 cut(s)	849, 1207, 1318, 1744, 1960, 2431
Bst6I	CTCTTC	5 cut(s)	248, 677, 1411, 2288, 2476
BstAFI	CTTAAG	1 cut(s)	2128
BstBAI	YACGTR	1 cut(s)	1244
BstBI	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
BstC8I	GCNNGC	8 cut(s)	428, 1108, 1384, 2042, 2093, 2436, 2733, 2774
BstDEI	CTNAG	8 cut(s)	299, 396, 513, 775, 1773, 1797, 2253, 2625
BstDSI	CCRYGG	1 cut(s)	2365
BstF5I	GGATG	9 cut(s)	859, 948, 1177, 1841, 2105, 2332, 2561, 2614, 2698
BstFNI	CGCG	3 cut(s)	109, 196, 562
BstH2I	RGCGCY	2 cut(s)	333, 675
BstHHI	GCGC	3 cut(s)	109, 332, 674
BstMAI	GTCTC	5 cut(s)	152, 1024, 2582, 2628, 2634
BstMCI	CGRYCG	1 cut(s)	32
BstNI	CCWGG	2 cut(s)	981, 2748
BstNSI	RCATGY	2 cut(s)	1231, 2095
BstSCI	CCNGG	6 cut(s)	169, 202, 210, 211, 979, 2746
BstUI	CGCG	3 cut(s)	109, 196, 562
BstV1I	GCAGC	4 cut(s)	64, 195, 394, 1225
BstV2I	GAAGAC	2 cut(s)	532, 1533
BstX2I	RGATCY	6 cut(s)	46, 289, 699, 766, 1388, 1791
BstYI	RGATCY	6 cut(s)	46, 289, 699, 766, 1388, 1791
BsuRI	GGCC	8 cut(s)	5, 74, 154, 210, 1122, 1488, 1953, 2104
BtgI	CCRYGG	1 cut(s)	2365
BtrI	CACGTC	2 cut(s)	10, 490
BtsCI	GGATG	9 cut(s)	859, 948, 1177, 1841, 2105, 2332, 2561, 2614, 2698
BtsI	GCAGTG	3 cut(s)	148, 386, 746
BveI	ACCTGC	1 cut(s)	423
Cac8I	GCNNGC	8 cut(s)	428, 1108, 1384, 2042, 2093, 2436, 2733, 2774
CaiI	CAGNNNCTG	2 cut(s)	385, 2513
CfoI	GCGC	3 cut(s)	109, 332, 674
Cfr13I	GGNCC	6 cut(s)	153, 209, 1013, 1750, 2240, 2579
Cfr9I	CCCGGG	1 cut(s)	211
CseI	GACGC	1 cut(s)	169
Csp6I	GTAC	3 cut(s)	692, 1241, 1314
CspCI	CAANNNNNGTGG	2 cut(s)	1632, 1667
CviAII	CATG	9 cut(s)	596, 604, 625, 1228, 1808, 2092, 2366, 2633, 2651
CviQI	GTAC	3 cut(s)	692, 1241, 1314
DdeI	CTNAG	8 cut(s)	299, 396, 513, 775, 1773, 1797, 2253, 2625
DraI	TTTAAA	1 cut(s)	957
DraIII	CACNNNGTG	1 cut(s)	1495
DriI	GACNNNNNGTC	1 cut(s)	1037
EaeI	YGGCCR	5 cut(s)	3, 1120, 1486, 1951, 2102
Eam1104I	CTCTTC	5 cut(s)	248, 677, 1411, 2288, 2476
Eam1105I	GACNNNNNGTC	1 cut(s)	1037
EarI	CTCTTC	5 cut(s)	248, 677, 1411, 2288, 2476
EciI	GGCGGA	1 cut(s)	80
Ecl136II	GAGCTC	1 cut(s)	298
Eco130I	CCWWGG	6 cut(s)	798, 1265, 1442, 2274, 2365, 2603
Eco147I	AGGCCT	1 cut(s)	74
Eco24I	GRGCYC	2 cut(s)	300, 1568
Eco31I	GGTCTC	1 cut(s)	1024
Eco32I	GATATC	1 cut(s)	1734
Eco47I	GGWCC	4 cut(s)	1013, 1750, 2240, 2579
Eco53kI	GAGCTC	1 cut(s)	298
Eco57I	CTGAAG	1 cut(s)	861
Eco88I	CYCGRG	2 cut(s)	155, 211
EcoICRI	GAGCTC	1 cut(s)	298
EcoO109I	RGGNCCY	1 cut(s)	1750
EcoRI	GAATTC	2 cut(s)	436, 1848
EcoRII	CCWGG	2 cut(s)	979, 2746
EcoRV	GATATC	1 cut(s)	1734
EcoT14I	CCWWGG	6 cut(s)	798, 1265, 1442, 2274, 2365, 2603
EcoT22I	ATGCAT	3 cut(s)	630, 1429, 2097
EcoT38I	GRGCYC	2 cut(s)	300, 1568
ErhI	CCWWGG	6 cut(s)	798, 1265, 1442, 2274, 2365, 2603
Esp3I	CGTCTC	1 cut(s)	152
FaeI	CATG	9 cut(s)	599, 607, 628, 1231, 1811, 2095, 2369, 2636, 2654
FalI	AAGNNNNNCTT	2 cut(s)	2189, 2221
FaqI	GGGAC	2 cut(s)	1424, 1435
FatI	CATG	9 cut(s)	595, 603, 624, 1227, 1807, 2091, 2365, 2632, 2650
FauI	CCCGC	1 cut(s)	1375
FauNDI	CATATG	1 cut(s)	1157
FbaI	TGATCA	1 cut(s)	2233
FblI	GTMKAC	1 cut(s)	2504
Fnu4HI	GCNGC	7 cut(s)	53, 184, 208, 408, 932, 1214, 1618
FokI	GGATG	9 cut(s)	866, 935, 1184, 1828, 2092, 2339, 2568, 2621, 2705
FriOI	GRGCYC	2 cut(s)	300, 1568
Fsp4HI	GCNGC	7 cut(s)	53, 184, 208, 408, 932, 1214, 1618
FspBI	CTAG	9 cut(s)	669, 936, 1091, 1107, 1400, 1854, 1896, 1941, 2118
GlaI	GCGC	3 cut(s)	108, 331, 673
GluI	GCNGC	7 cut(s)	53, 184, 208, 408, 932, 1214, 1618
GsaI	CCCAGC	1 cut(s)	1824
GsuI	CTGGAG	1 cut(s)	669
HaeII	RGCGCY	2 cut(s)	333, 675
HaeIII	GGCC	8 cut(s)	5, 74, 154, 210, 1122, 1488, 1953, 2104
HapII	CCGG	9 cut(s)	20, 151, 170, 204, 212, 293, 1055, 1119, 2101
HgaI	GACGC	1 cut(s)	169
HhaI	GCGC	3 cut(s)	109, 332, 674
Hin1II	CATG	9 cut(s)	599, 607, 628, 1231, 1811, 2095, 2369, 2636, 2654
Hin6I	GCGC	3 cut(s)	107, 330, 672
HinP1I	GCGC	3 cut(s)	107, 330, 672
HincII	GTYRAC	1 cut(s)	2505
HindII	GTYRAC	1 cut(s)	2505
HpaII	CCGG	9 cut(s)	20, 151, 170, 204, 212, 293, 1055, 1119, 2101
HphI	GGTGA	4 cut(s)	1728, 2381, 2656, 2734
Hpy166II	GTNNAC	7 cut(s)	200, 610, 641, 694, 829, 2165, 2505
Hpy8I	GTNNAC	7 cut(s)	200, 610, 641, 694, 829, 2165, 2505
Hpy99I	CGWCG	1 cut(s)	11
HpyAV	CCTTC	9 cut(s)	725, 1052, 1453, 1513, 1741, 1930, 2077, 2554, 2716
HpyCH4III	ACNGT	6 cut(s)	849, 1207, 1318, 1744, 1960, 2431
HpyCH4IV	ACGT	5 cut(s)	9, 489, 1019, 1243, 1930
HpyF3I	CTNAG	8 cut(s)	299, 396, 513, 775, 1773, 1797, 2253, 2625
HpySE526I	ACGT	5 cut(s)	9, 489, 1019, 1243, 1930
Hsp92II	CATG	9 cut(s)	599, 607, 628, 1231, 1811, 2095, 2369, 2636, 2654
HspAI	GCGC	3 cut(s)	107, 330, 672
Kpn2I	TCCGGA	2 cut(s)	292, 1054
Ksp22I	TGATCA	1 cut(s)	2233
LguI	GCTCTTC	1 cut(s)	2476
LmnI	GCTCC	6 cut(s)	174, 245, 295, 749, 1046, 2035
Lsp1109I	GCAGC	4 cut(s)	64, 195, 394, 1225
LweI	GCATC	8 cut(s)	439, 580, 875, 1414, 1436, 1475, 1778, 2530
MaeI	CTAG	9 cut(s)	669, 936, 1091, 1107, 1400, 1854, 1896, 1941, 2118
MaeII	ACGT	5 cut(s)	9, 489, 1019, 1243, 1930
MaeIII	GTNAC	4 cut(s)	143, 257, 2002, 2479
MbiI	CCGCTC	1 cut(s)	177
MfeI	CAATTG	3 cut(s)	2527, 2592, 2791
MflI	RGATCY	6 cut(s)	46, 289, 699, 766, 1388, 1791
MhlI	GDGCHC	4 cut(s)	300, 746, 1568, 2354
MlsI	TGGCCA	2 cut(s)	1488, 1953
MluNI	TGGCCA	2 cut(s)	1488, 1953
MlyI	GAGTC	1 cut(s)	2510
MmeI	TCCRAC	3 cut(s)	246, 831, 1002
Mox20I	TGGCCA	2 cut(s)	1488, 1953
Mph1103I	ATGCAT	3 cut(s)	630, 1429, 2097
MroI	TCCGGA	2 cut(s)	292, 1054
MroXI	GAANNNNTTC	2 cut(s)	116, 1362
MscI	TGGCCA	2 cut(s)	1488, 1953
Msp20I	TGGCCA	2 cut(s)	1488, 1953
MspCI	CTTAAG	1 cut(s)	2128
MspI	CCGG	9 cut(s)	20, 151, 170, 204, 212, 293, 1055, 1119, 2101
MspR9I	CCNGG	6 cut(s)	171, 204, 212, 213, 981, 2748
MunI	CAATTG	3 cut(s)	2527, 2592, 2791
MvaI	CCWGG	2 cut(s)	981, 2748
MvnI	CGCG	3 cut(s)	109, 196, 562
NciI	CCSGG	4 cut(s)	171, 204, 212, 213
NcoI	CCATGG	1 cut(s)	2365
NdeI	CATATG	1 cut(s)	1157
NheI	GCTAGC	1 cut(s)	1106
NlaIII	CATG	9 cut(s)	599, 607, 628, 1231, 1811, 2095, 2369, 2636, 2654
NlaIV	GGNNCC	4 cut(s)	48, 291, 1669, 1752
NmuCI	GTSAC	3 cut(s)	143, 257, 2002
NsiI	ATGCAT	3 cut(s)	630, 1429, 2097
NspI	RCATGY	2 cut(s)	1231, 2095
NspV	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
PaeI	GCATGC	1 cut(s)	2095
PceI	AGGCCT	1 cut(s)	74
PciI	ACATGT	1 cut(s)	1227
PciSI	GCTCTTC	1 cut(s)	2476
PcsI	WCGNNNNNNNCGW	1 cut(s)	83
PdmI	GAANNNNTTC	2 cut(s)	116, 1362
PflMI	CCANNNNNTGG	2 cut(s)	1495, 1960
PkrI	GCNGC	7 cut(s)	54, 185, 209, 409, 933, 1215, 1619
Ple19I	CGATCG	1 cut(s)	32
PleI	GAGTC	1 cut(s)	2509
PpsI	GAGTC	1 cut(s)	2509
Ppu21I	YACGTR	1 cut(s)	1244
PpuMI	RGGWCCY	1 cut(s)	1750
PscI	ACATGT	1 cut(s)	1227
Psp124BI	GAGCTC	1 cut(s)	300
Psp5II	RGGWCCY	1 cut(s)	1750
Psp6I	CCWGG	2 cut(s)	979, 2746
PspFI	CCCAGC	1 cut(s)	1820
PspGI	CCWGG	2 cut(s)	979, 2746
PspN4I	GGNNCC	4 cut(s)	48, 291, 1669, 1752
PspPI	GGNCC	6 cut(s)	153, 209, 1013, 1750, 2240, 2579
PspPPI	RGGWCCY	1 cut(s)	1750
PsrI	GAACNNNNNNTAC	2 cut(s)	1176, 1208
PstNI	CAGNNNCTG	2 cut(s)	385, 2513
PsuI	RGATCY	6 cut(s)	46, 289, 699, 766, 1388, 1791
PvuI	CGATCG	1 cut(s)	32
RsaI	GTAC	3 cut(s)	693, 1242, 1315
RsaNI	GTAC	3 cut(s)	692, 1241, 1314
SacI	GAGCTC	1 cut(s)	300
SalI	GTCGAC	1 cut(s)	2503
SapI	GCTCTTC	1 cut(s)	2476
SatI	GCNGC	7 cut(s)	53, 184, 208, 408, 932, 1214, 1618
Sau96I	GGNCC	6 cut(s)	153, 209, 1013, 1750, 2240, 2579
SchI	GAGTC	1 cut(s)	2510
ScrFI	CCNGG	6 cut(s)	171, 204, 212, 213, 981, 2748
SduI	GDGCHC	4 cut(s)	300, 746, 1568, 2354
SfaNI	GCATC	8 cut(s)	439, 580, 875, 1414, 1436, 1475, 1778, 2530
SfuI	TTCGAA	5 cut(s)	718, 1141, 1366, 1544, 2291
SinI	GGWCC	4 cut(s)	1013, 1750, 2240, 2579
SmaI	CCCGGG	1 cut(s)	213
SmlI	CTYRAG	2 cut(s)	1027, 2128
SmoI	CTYRAG	2 cut(s)	1027, 2128
SphI	GCATGC	1 cut(s)	2095
SseBI	AGGCCT	1 cut(s)	74
SspI	AATATT	1 cut(s)	1258
SspMI	CTAG	9 cut(s)	669, 936, 1091, 1107, 1400, 1854, 1896, 1941, 2118
SstI	GAGCTC	1 cut(s)	300
StuI	AGGCCT	1 cut(s)	74
StyD4I	CCNGG	6 cut(s)	169, 202, 210, 211, 979, 2746
StyI	CCWWGG	6 cut(s)	798, 1265, 1442, 2274, 2365, 2603
TaaI	ACNGT	6 cut(s)	849, 1207, 1318, 1744, 1960, 2431
TaiI	ACGT	5 cut(s)	12, 492, 1022, 1246, 1933
TauI	GCSGC	3 cut(s)	210, 934, 1620
TseFI	GTSAC	3 cut(s)	143, 257, 2002
TseI	GCWGC	4 cut(s)	52, 183, 407, 1213
Tsp45I	GTSAC	3 cut(s)	143, 257, 2002
TspDTI	ATGAA	9 cut(s)	89, 612, 923, 1085, 1830, 1941, 2217, 2343, 2709
TspGWI	ACGGA	1 cut(s)	2339
TspMI	CCCGGG	1 cut(s)	211
Van91I	CCANNNNNTGG	2 cut(s)	1495, 1960
Vha464I	CTTAAG	1 cut(s)	2128
VpaK11BI	GGWCC	4 cut(s)	1013, 1750, 2240, 2579
XapI	RAATTY	3 cut(s)	436, 1825, 1848
XbaI	TCTAGA	1 cut(s)	1895
XceI	RCATGY	2 cut(s)	1231, 2095
XmaI	CCCGGG	1 cut(s)	211
XmiI	GTMKAC	1 cut(s)	2504
XmnI	GAANNNNTTC	2 cut(s)	116, 1362
XspI	CTAG	9 cut(s)	669, 936, 1091, 1107, 1400, 1854, 1896, 1941, 2118
Zsp2I	ATGCAT	3 cut(s)	630, 1429, 2097

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.