Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0009301

Physical Location & Seq

Genomic Coordinates Reverse (-)

933475 .. 938695

Gene Structure 5221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015716

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2859 bp

ATGGCCGACGTGTTCAATCCGGCAATCCGATCGGGTCAGAGTTCGGATCCGCAGCTCAAATCGGCGGAGGAGGCCTCGCTGTTTCCGAGGATCGAGTTTCATCTGGCGCGGAGGCCATTCAAGGGCTTTAACAATGACGGCAGTGACTTCCGGCCCGAGACGCTTAATCCGGGGAGCGGTGGCAGCTCGGATTCGCGTAAACCCGGCGGCCCGGGAGGTCAGGGTCAGTCGGCTTCGGGTTCGGGTTCGGGAGCGAAGAAGAGTGACGGGTCGGAGTTTTTGGAGAACGGGTGGGATCCGGAGCTCAGCTTTCCCATCACATTTCGGAGAATTGGTGCGGGTTTGATAAATATGGGGAATACTTGTTATCTCAATTCAGTGTTGCAGTGCCTGACATATACTGAGCCTTTAGCTGCATACCTGCAAAGTGGCAGGCATCGGAATTCTTGTCATATAAAAAATGGGTTTTGTGCTTTATGTGCCATCCAAGAACACGTCAGTCTTGCGCTGCAATCAACTAAGAGCATTTTGCCAAAGGCTCTTGTCTTCAATCTACAACGCATATCACGGAACTTTAGGAAGTCCAGACAGGAAGATGCACATGAATACATGGTAAACTTGCTGGAATCCATGCATAAATGTTGTTTACCATCTGGAGTGCCAAGTGAATCCCCTAGTGCCTATGAGAAGAGTTTGGTACACAAGATCTTTGGTGGTCGCCTTCGAAGTCGGGTCAAATGTTTGCAGTGCTCCTATTGCTCTGACAAGTTAGATCCATTCTTAGATTTAAGTCTTGAAATAGCCAAGGCAAACTCTTTGAATAAGGCACTTGTGAACTTTACTGCTGAAGAACAGTTGGATGGTGGGGAAAGGCAATATCAGTGCCAAAGATGCAATCAGAAAGTTCGAGCTATGAAACAGATGACCATTCACAAGCCGCCTAGTGTTCTGTCCATCCATTTAAAGCGGTTTCGTGCATATGACCCCGGACAAAAAATTAACAAAGAAGTTCAGTTTGGTCCTACGTTGGACTTGAGACCTTTTGTCAGTGGAGCATATCCGGAAGGAGATTTGAAATATACTCTTTATGGGGTTCTAGTTCATTGTGGGGCTAGCACCTATTCCGGCCATTATTATTGCTTTGTTCGAACGTCAACTGGCATATGGCACTCTCTGGATGACAACAATGTAAGGCAAGTTCGTGAGATGACTGTTTTGCAGCAAAAGGCTTACATGTTGTTCTATGTACGTGATAGAAGAAATATTATTCCAAGGAAGCCTGTTGATGTTGCTCAAAAGGAGTATTTAAAACCTAATGGTACTGTAAATAAAATTACTCCTACTGACAACCATTTGTCGAAAGAACCTATTCGAAAAATTGCAGTTGCGGGCAGATCCAATGATCTATCCTCTTCTGTTGCACAAAAAGATGCATCAGCTATTGTCCCAAGGGTGTCCCATTTGAAGGAAGCATCAGTTAATCAAAATAATGGCCACATGGTGGCACAAAATATGGTGCGTAAGAAGGAAGCCATTTCAGAATCGTCTTCGAAAGCGTCATTATCAGAGGGCTCATTGGGAGGATTAGGAGAAAATATGCCACCATCTACTCTGCTTCCAAGCCGCAAAACTGGCACTTCTGATTCTGCAAGTGCTGTGGCTTCAACAACTGGCGCCAAACTAAATAACTGCAATGAGAAAGCAAGTTCAAATGATAATGATAGGGTTTCAATTATGATATCACCTACTGTGAAGGACCCTGAAATCTTAAAGGCATCTAAGCCTGTTCAAGATGAGATCCCTCAGAATAAGAATCATGCTTCCGGTGCTGGGAATTTGAGTGTGGCTTCATCCAGAATTCCTAGTGGTTTAACAATGGAGAAGCTGAATCCTGAAAAGCCATCTGATCCACCAAGCTCTAGAATCAGTCAGGCTGGAAGTTTTACCAATGAACGTGAGAAGGCTAGTGATTGTGGCCAGACAGTGGTAGTTTCTGATTCTGCAAAATTGTCTGGCTCATCAGTTGTGACAAATAGATCATTAGATTTGGAAGCTCCTGACCGCAAGCACCCTAAAGGATTGAAAAAGAAGCACATAAAGTGTAAGGTGGTAGGCATGCATTTCCGGCCATCCTTGCTTTCTAGGGCAGTCTTAAGAGTGCAGAAGAAGAAGAAGCACAAAAGGAGTAAACGCCCAACTTCAGATTGTAAGAGCCATAGTAAGAAATACTTGGTAGAAAATTATTGTTCTTCATCTGATCAAGGACCATCTACATCTGAGAAAACCCAGACAGATTCCTTGGTTTCTGCCTGTTCGAAGAGAAAGAGAAACAAATCTGGCTTGAAAACGGATGAAGATGGAACTTCTAATAAGTGCTCACTGAATCCCCATGGTGATTCTTCGATGGATATTAAAGATGTAGAGTTTAGAGAGAGCCTCACTCAGAATGGAACAGTGCTTGCAAGTGATAAACAACAACAAAATGGTTTTGGCTCTTCCTCTGTTACATTGGAAAACAAAAGGGAGTCGAGAGGAACTGATACTCCACACAATTGTAAAAGAGATGCAATGCAAAATGGATGGAAGGGTGTGCTGACTCAAGCTCCAGCGGAGACAATTGTTGCACCTTGGGATGGAATAGAATTGGCTCAGTCTCATGTTGTAGAATCAAGCCATGCACAGAGTATCACCATCGGTTATGTAGGAGATGATTGGGATGAAGAATATGATCGGGGAAAGAGAAAGAAGGTAAGGCAAGCAAAACTCACCCCTGGTGGGCCAAATCCTTTCCAAATACTTGCAAACAAGAAAGCACAATTGAAGCAACCAAGGATACAAAGGTTTGATTCTGGAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH

Protein Analysis

953

Amino Acids

104.47

Weight (kDa)

9.46

Isoelectric Point (pI)

50.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	113 - 415	1.2e-61	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	113 - 396	1.2e-15	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57990
fragaria_vesca	FvH4_1g01010 FvH4_1g01010 FvH4_1g01010
malus_domestica	MD02G1008300.v1.1 MD15G1152500.v1.1
prunus_persica	Prupe.7G262200_v2.0.a1
pyrus_communis	pycom02g00720 pycom15g13710
rosa_chinensis	RchiOBHm_Chr2g0085751
rosa_laevigata	RLG00000015716
rosa_multiflora	Rmu_sc0009759.1_g000004 Rmu_sc0023131.1_g000003 Rmu_sc0033740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155070
rosa_rugosa	Rorug01G0462900 Rorug01G0462900
rosa_samantha	Rh2AG012000 Rh2BG012600 Rh2CG013400 Rh2DG014400
rosa_wichuraiana	Rw2G001070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	429
AccB1I	GGYRCC	1 cut(s)	1673
AccB7I	CCANNNNNTGG	2 cut(s)	1501, 1984
AccBSI	CCGCTC	1 cut(s)	177
AccII	CGCG	2 cut(s)	109, 196
AccIII	TCCGGA	2 cut(s)	298, 1060
AclWI	GGATC	9 cut(s)	41, 54, 98, 290, 303, 767, 1389, 1792, 1901
AcoI	YGGCCR	5 cut(s)	3, 1126, 1492, 1975, 2126
AcsI	RAATTY	3 cut(s)	442, 1834, 1857
AcuI	CTGAAG	2 cut(s)	867, 2184
AcyI	GRCGYC	1 cut(s)	1674
AdeI	CACNNNGTG	1 cut(s)	1501
AfaI	GTAC	3 cut(s)	699, 1248, 1321
AfiI	CCNNNNNNNGG	8 cut(s)	122, 176, 1465, 1501, 1830, 1984, 2633, 2775
AflII	CTTAAG	1 cut(s)	2152
AflIII	ACRYGT	3 cut(s)	9, 493, 1233
AhdI	GACNNNNNGTC	1 cut(s)	1043
AjiI	CACGTC	2 cut(s)	10, 496
AjnI	CCWGG	1 cut(s)	2770
AjuI	GAANNNNNNNTTGG	2 cut(s)	999, 1031
AloI	GAACNNNNNNTCC	2 cut(s)	2527, 2559
Alw21I	GWGCWC	3 cut(s)	306, 752, 2378
Alw26I	GTCTC	4 cut(s)	152, 1030, 2606, 2658
AlwI	GGATC	9 cut(s)	41, 54, 98, 290, 303, 767, 1389, 1792, 1901
AlwNI	CAGNNNCTG	1 cut(s)	391
Ama87I	CYCGRG	2 cut(s)	155, 211
Aor13HI	TCCGGA	2 cut(s)	298, 1060
ApeKI	GCWGC	5 cut(s)	52, 183, 413, 508, 1219
ApoI	RAATTY	3 cut(s)	442, 1834, 1857
Asp700I	GAANNNNTTC	1 cut(s)	1368
AspLEI	GCGC	3 cut(s)	109, 508, 1676
AspS9I	GGNCC	6 cut(s)	153, 209, 1019, 1756, 2264, 2777
AsuC2I	CCSGG	5 cut(s)	171, 204, 212, 213, 987
AsuHPI	GGTGA	4 cut(s)	1734, 2405, 2680, 2758
AsuII	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
AsuNHI	GCTAGC	1 cut(s)	1112
AvaI	CYCGRG	2 cut(s)	155, 211
AvaII	GGWCC	3 cut(s)	1019, 1756, 2264
BaeI	ACNNNNGTAYC	2 cut(s)	2825, 2858
BalI	TGGCCA	2 cut(s)	1494, 1977
BamHI	GGATCC	2 cut(s)	46, 295
BanI	GGYRCC	1 cut(s)	1673
BanII	GRGCYC	2 cut(s)	306, 1574
BbsI	GAAGAC	2 cut(s)	538, 1539
Bbv12I	GWGCWC	3 cut(s)	306, 752, 2378
BbvI	GCAGC	5 cut(s)	64, 195, 400, 495, 1231
BceAI	ACGGC	1 cut(s)	154
BcgI	CGANNNNNNTGC	2 cut(s)	12, 46
BciT130I	CCWGG	1 cut(s)	2772
BciVI	GTATCC	1 cut(s)	2826
BclI	TGATCA	1 cut(s)	2257
BcnI	CCSGG	5 cut(s)	171, 204, 212, 213, 987
BcoDI	GTCTC	4 cut(s)	152, 1030, 2606, 2658
BfaI	CTAG	8 cut(s)	675, 942, 1097, 1113, 1863, 1920, 1965, 2142
BfoI	RGCGCY	1 cut(s)	1677
BfrI	CTTAAG	1 cut(s)	2152
BfuAI	ACCTGC	1 cut(s)	429
BfuI	GTATCC	1 cut(s)	2826
BglII	AGATCT	1 cut(s)	705
BisI	GCNGC	8 cut(s)	53, 184, 208, 414, 509, 938, 1220, 1624
BlpI	GCTNAGC	1 cut(s)	305
BlsI	GCNGC	8 cut(s)	54, 185, 209, 415, 510, 939, 1221, 1625
Bme1390I	CCNGG	6 cut(s)	171, 204, 212, 213, 987, 2772
Bme18I	GGWCC	3 cut(s)	1019, 1756, 2264
BmeRI	GACNNNNNGTC	1 cut(s)	1043
BmeT110I	CYCGRG	2 cut(s)	155, 211
BmgBI	CACGTC	2 cut(s)	10, 496
BmgT120I	GGNCC	6 cut(s)	153, 209, 1019, 1756, 2264, 2777
BmiI	GGNNCC	4 cut(s)	48, 297, 1675, 1758
BmrFI	CCNGG	6 cut(s)	171, 204, 212, 213, 987, 2772
BmsI	GCATC	8 cut(s)	445, 586, 881, 1420, 1442, 1481, 1784, 2554
BmtI	GCTAGC	1 cut(s)	1116
BpiI	GAAGAC	2 cut(s)	538, 1539
BplI	GAGNNNNNCTC	6 cut(s)	59, 91, 2421, 2453, 2425, 2457
BpmI	CTGGAG	2 cut(s)	675, 2589
Bpu1102I	GCTNAGC	1 cut(s)	305
Bpu14I	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
BpuEI	CTTGAG	2 cut(s)	1054, 2583
BpuMI	CCSGG	5 cut(s)	171, 204, 212, 213, 987
BsaAI	YACGTR	1 cut(s)	1250
BsaHI	GRCGYC	1 cut(s)	1674
BsaI	GGTCTC	1 cut(s)	1030
BsaWI	WCCGGW	3 cut(s)	298, 1060, 1823
BsaXI	ACNNNNNCTCC	8 cut(s)	275, 305, 319, 349, 2527, 2557, 2587, 2617
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 176, 1465, 1501, 1830, 1984, 2633, 2775
Bse1I	ACTGG	3 cut(s)	1162, 1636, 1675
Bse3DI	GCAATG	2 cut(s)	1699, 2574
BseAI	TCCGGA	2 cut(s)	298, 1060
BseBI	CCWGG	1 cut(s)	2772
BseLI	CCNNNNNNNGG	8 cut(s)	122, 176, 1465, 1501, 1830, 1984, 2633, 2775
BseMI	GCAATG	2 cut(s)	1699, 2574
BseMII	CTCAG	6 cut(s)	319, 393, 1817, 2268, 2456, 2663
BseNI	ACTGG	3 cut(s)	1162, 1636, 1675
BseRI	GAGGAG	1 cut(s)	83
BseXI	GCAGC	5 cut(s)	64, 195, 400, 495, 1231
BseYI	CCCAGC	1 cut(s)	1829
BsgI	GTGCAG	1 cut(s)	2180
Bsh1236I	CGCG	2 cut(s)	109, 196
Bsh1285I	CGRYCG	1 cut(s)	32
BshNI	GGYRCC	1 cut(s)	1673
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	3 cut(s)	306, 752, 2378
BsiHKCI	CYCGRG	2 cut(s)	155, 211
BslFI	GGGAC	2 cut(s)	1430, 1441
BslI	CCNNNNNNNGG	8 cut(s)	122, 176, 1465, 1501, 1830, 1984, 2633, 2775
BsmAI	GTCTC	4 cut(s)	152, 1030, 2606, 2658
BsmBI	CGTCTC	1 cut(s)	152
BsmFI	GGGAC	2 cut(s)	1430, 1441
Bso31I	GGTCTC	1 cut(s)	1030
BsoBI	CYCGRG	2 cut(s)	155, 211
Bsp119I	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
Bsp1286I	GDGCHC	4 cut(s)	306, 752, 1574, 2378
Bsp13I	TCCGGA	2 cut(s)	298, 1060
Bsp1720I	GCTNAGC	1 cut(s)	305
Bsp19I	CCATGG	1 cut(s)	2389
BspCNI	CTCAG	6 cut(s)	318, 394, 1816, 2269, 2455, 2662
BspEI	TCCGGA	2 cut(s)	298, 1060
BspFNI	CGCG	2 cut(s)	109, 196
BspLI	GGNNCC	4 cut(s)	48, 297, 1675, 1758
BspMI	ACCTGC	1 cut(s)	429
BspOI	GCTAGC	1 cut(s)	1116
BspPI	GGATC	9 cut(s)	41, 54, 98, 290, 303, 767, 1389, 1792, 1901
BspQI	GCTCTTC	1 cut(s)	2500
BspT104I	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
BspT107I	GGYRCC	1 cut(s)	1673
BspTI	CTTAAG	1 cut(s)	2152
BspTNI	GGTCTC	1 cut(s)	1030
BsrBI	CCGCTC	1 cut(s)	177
BsrDI	GCAATG	2 cut(s)	1699, 2574
BsrI	ACTGG	3 cut(s)	1162, 1636, 1675
BssNI	GRCGYC	1 cut(s)	1674
BssT1I	CCWWGG	7 cut(s)	804, 1271, 1448, 2298, 2389, 2627, 2828
Bst2UI	CCWGG	1 cut(s)	2772
Bst4CI	ACNGT	6 cut(s)	855, 1213, 1324, 1750, 1984, 2455
Bst6I	CTCTTC	5 cut(s)	254, 683, 1417, 2312, 2500
BstACI	GRCGYC	1 cut(s)	1674
BstAFI	CTTAAG	1 cut(s)	2152
BstBAI	YACGTR	1 cut(s)	1250
BstBI	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
BstC8I	GCNNGC	7 cut(s)	434, 1114, 1390, 2066, 2117, 2460, 2757
BstDEI	CTNAG	9 cut(s)	305, 402, 519, 781, 1779, 1803, 2277, 2442, 2649
BstDSI	CCRYGG	1 cut(s)	2389
BstFNI	CGCG	2 cut(s)	109, 196
BstH2I	RGCGCY	1 cut(s)	1677
BstHHI	GCGC	3 cut(s)	109, 508, 1676
BstMAI	GTCTC	4 cut(s)	152, 1030, 2606, 2658
BstMCI	CGRYCG	1 cut(s)	32
BstNI	CCWGG	1 cut(s)	2772
BstNSI	RCATGY	2 cut(s)	1237, 2119
BstSCI	CCNGG	6 cut(s)	169, 202, 210, 211, 985, 2770
BstUI	CGCG	2 cut(s)	109, 196
BstV1I	GCAGC	5 cut(s)	64, 195, 400, 495, 1231
BstV2I	GAAGAC	2 cut(s)	538, 1539
BstX2I	RGATCY	6 cut(s)	46, 295, 705, 772, 1394, 1797
BstYI	RGATCY	6 cut(s)	46, 295, 705, 772, 1394, 1797
BsuI	GTATCC	1 cut(s)	2826
BtgI	CCRYGG	1 cut(s)	2389
BtrI	CACGTC	2 cut(s)	10, 496
BtsI	GCAGTG	3 cut(s)	148, 392, 752
BtsIMutI	CAGTG	9 cut(s)	148, 384, 392, 752, 887, 1054, 1989, 2378, 2460
BveI	ACCTGC	1 cut(s)	429
Cac8I	GCNNGC	7 cut(s)	434, 1114, 1390, 2066, 2117, 2460, 2757
CaiI	CAGNNNCTG	1 cut(s)	391
CfoI	GCGC	3 cut(s)	109, 508, 1676
Cfr13I	GGNCC	6 cut(s)	153, 209, 1019, 1756, 2264, 2777
Cfr9I	CCCGGG	1 cut(s)	211
CseI	GACGC	2 cut(s)	169, 1545
Csp6I	GTAC	3 cut(s)	698, 1247, 1320
CspCI	CAANNNNNGTGG	2 cut(s)	1638, 1673
CviQI	GTAC	3 cut(s)	698, 1247, 1320
DdeI	CTNAG	9 cut(s)	305, 402, 519, 781, 1779, 1803, 2277, 2442, 2649
DinI	GGCGCC	1 cut(s)	1675
DraI	TTTAAA	2 cut(s)	963, 1308
DraIII	CACNNNGTG	1 cut(s)	1501
DriI	GACNNNNNGTC	1 cut(s)	1043
EaeI	YGGCCR	5 cut(s)	3, 1126, 1492, 1975, 2126
Eam1104I	CTCTTC	5 cut(s)	254, 683, 1417, 2312, 2500
Eam1105I	GACNNNNNGTC	1 cut(s)	1043
EarI	CTCTTC	5 cut(s)	254, 683, 1417, 2312, 2500
EciI	GGCGGA	1 cut(s)	80
Ecl136II	GAGCTC	1 cut(s)	304
Eco130I	CCWWGG	7 cut(s)	804, 1271, 1448, 2298, 2389, 2627, 2828
Eco147I	AGGCCT	1 cut(s)	74
Eco24I	GRGCYC	2 cut(s)	306, 1574
Eco31I	GGTCTC	1 cut(s)	1030
Eco32I	GATATC	1 cut(s)	1740
Eco47I	GGWCC	3 cut(s)	1019, 1756, 2264
Eco53kI	GAGCTC	1 cut(s)	304
Eco57I	CTGAAG	2 cut(s)	867, 2184
Eco88I	CYCGRG	2 cut(s)	155, 211
EcoICRI	GAGCTC	1 cut(s)	304
EcoO109I	RGGNCCY	1 cut(s)	1756
EcoRI	GAATTC	2 cut(s)	442, 1857
EcoRII	CCWGG	1 cut(s)	2770
EcoRV	GATATC	1 cut(s)	1740
EcoT14I	CCWWGG	7 cut(s)	804, 1271, 1448, 2298, 2389, 2627, 2828
EcoT22I	ATGCAT	3 cut(s)	636, 1435, 2121
EcoT38I	GRGCYC	2 cut(s)	306, 1574
EgeI	GGCGCC	1 cut(s)	1675
EheI	GGCGCC	1 cut(s)	1675
ErhI	CCWWGG	7 cut(s)	804, 1271, 1448, 2298, 2389, 2627, 2828
Esp3I	CGTCTC	1 cut(s)	152
FalI	AAGNNNNNCTT	2 cut(s)	2213, 2245
FaqI	GGGAC	2 cut(s)	1430, 1441
FauI	CCCGC	2 cut(s)	331, 1381
FauNDI	CATATG	2 cut(s)	979, 1163
FbaI	TGATCA	1 cut(s)	2257
Fnu4HI	GCNGC	8 cut(s)	53, 184, 208, 414, 509, 938, 1220, 1624
FriOI	GRGCYC	2 cut(s)	306, 1574
Fsp4HI	GCNGC	8 cut(s)	53, 184, 208, 414, 509, 938, 1220, 1624
FspBI	CTAG	8 cut(s)	675, 942, 1097, 1113, 1863, 1920, 1965, 2142
GlaI	GCGC	3 cut(s)	108, 507, 1675
GluI	GCNGC	8 cut(s)	53, 184, 208, 414, 509, 938, 1220, 1624
GsaI	CCCAGC	1 cut(s)	1833
GsuI	CTGGAG	2 cut(s)	675, 2589
HaeII	RGCGCY	1 cut(s)	1677
HgaI	GACGC	2 cut(s)	169, 1545
HhaI	GCGC	3 cut(s)	109, 508, 1676
Hin1I	GRCGYC	1 cut(s)	1674
Hin6I	GCGC	3 cut(s)	107, 506, 1674
HinP1I	GCGC	3 cut(s)	107, 506, 1674
HincII	GTYRAC	1 cut(s)	1155
HindII	GTYRAC	1 cut(s)	1155
HphI	GGTGA	4 cut(s)	1734, 2405, 2680, 2758
Hpy166II	GTNNAC	7 cut(s)	200, 616, 647, 700, 835, 1155, 2189
Hpy8I	GTNNAC	7 cut(s)	200, 616, 647, 700, 835, 1155, 2189
Hpy99I	CGWCG	1 cut(s)	11
HpyAV	CCTTC	8 cut(s)	731, 1058, 1459, 1519, 1747, 1954, 2578, 2740
HpyCH4III	ACNGT	6 cut(s)	855, 1213, 1324, 1750, 1984, 2455
HpyCH4IV	ACGT	6 cut(s)	9, 495, 1025, 1151, 1249, 1954
HpyF3I	CTNAG	9 cut(s)	305, 402, 519, 781, 1779, 1803, 2277, 2442, 2649
HpySE526I	ACGT	6 cut(s)	9, 495, 1025, 1151, 1249, 1954
Hsp92I	GRCGYC	1 cut(s)	1674
HspAI	GCGC	3 cut(s)	107, 506, 1674
KasI	GGCGCC	1 cut(s)	1673
Kpn2I	TCCGGA	2 cut(s)	298, 1060
Ksp22I	TGATCA	1 cut(s)	2257
LguI	GCTCTTC	1 cut(s)	2500
LmnI	GCTCC	7 cut(s)	174, 251, 301, 755, 1052, 2059, 2608
Lsp1109I	GCAGC	5 cut(s)	64, 195, 400, 495, 1231
LweI	GCATC	8 cut(s)	445, 586, 881, 1420, 1442, 1481, 1784, 2554
MaeI	CTAG	8 cut(s)	675, 942, 1097, 1113, 1863, 1920, 1965, 2142
MaeII	ACGT	6 cut(s)	9, 495, 1025, 1151, 1249, 1954
MaeIII	GTNAC	4 cut(s)	143, 263, 2026, 2503
MbiI	CCGCTC	1 cut(s)	177
MfeI	CAATTG	3 cut(s)	2551, 2616, 2816
MflI	RGATCY	6 cut(s)	46, 295, 705, 772, 1394, 1797
MhlI	GDGCHC	4 cut(s)	306, 752, 1574, 2378
MlsI	TGGCCA	2 cut(s)	1494, 1977
MluNI	TGGCCA	2 cut(s)	1494, 1977
Mly113I	GGCGCC	1 cut(s)	1674
MlyI	GAGTC	2 cut(s)	2534, 2590
MmeI	TCCRAC	3 cut(s)	252, 837, 1008
Mox20I	TGGCCA	2 cut(s)	1494, 1977
Mph1103I	ATGCAT	3 cut(s)	636, 1435, 2121
MroI	TCCGGA	2 cut(s)	298, 1060
MroXI	GAANNNNTTC	1 cut(s)	1368
MscI	TGGCCA	2 cut(s)	1494, 1977
Msp20I	TGGCCA	2 cut(s)	1494, 1977
MspA1I	CMGCKG	1 cut(s)	2609
MspCI	CTTAAG	1 cut(s)	2152
MspR9I	CCNGG	6 cut(s)	171, 204, 212, 213, 987, 2772
MunI	CAATTG	3 cut(s)	2551, 2616, 2816
MvaI	CCWGG	1 cut(s)	2772
MvnI	CGCG	2 cut(s)	109, 196
NarI	GGCGCC	1 cut(s)	1674
NciI	CCSGG	5 cut(s)	171, 204, 212, 213, 987
NcoI	CCATGG	1 cut(s)	2389
NdeI	CATATG	2 cut(s)	979, 1163
NheI	GCTAGC	1 cut(s)	1112
NlaIV	GGNNCC	4 cut(s)	48, 297, 1675, 1758
NmuCI	GTSAC	3 cut(s)	143, 263, 2026
NsiI	ATGCAT	3 cut(s)	636, 1435, 2121
NspI	RCATGY	2 cut(s)	1237, 2119
NspV	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
PaeI	GCATGC	1 cut(s)	2119
PceI	AGGCCT	1 cut(s)	74
PciI	ACATGT	1 cut(s)	1233
PciSI	GCTCTTC	1 cut(s)	2500
PcsI	WCGNNNNNNNCGW	1 cut(s)	83
PdmI	GAANNNNTTC	1 cut(s)	1368
PflMI	CCANNNNNTGG	2 cut(s)	1501, 1984
PkrI	GCNGC	8 cut(s)	54, 185, 209, 415, 510, 939, 1221, 1625
Ple19I	CGATCG	1 cut(s)	32
PleI	GAGTC	2 cut(s)	2533, 2590
PluTI	GGCGCC	1 cut(s)	1677
PpsI	GAGTC	2 cut(s)	2533, 2590
Ppu21I	YACGTR	1 cut(s)	1250
PpuMI	RGGWCCY	1 cut(s)	1756
PscI	ACATGT	1 cut(s)	1233
Psp124BI	GAGCTC	1 cut(s)	306
Psp5II	RGGWCCY	1 cut(s)	1756
Psp6I	CCWGG	1 cut(s)	2770
PspFI	CCCAGC	1 cut(s)	1829
PspGI	CCWGG	1 cut(s)	2770
PspN4I	GGNNCC	4 cut(s)	48, 297, 1675, 1758
PspPI	GGNCC	6 cut(s)	153, 209, 1019, 1756, 2264, 2777
PspPPI	RGGWCCY	1 cut(s)	1756
PsrI	GAACNNNNNNTAC	2 cut(s)	1182, 1214
PstNI	CAGNNNCTG	1 cut(s)	391
PsuI	RGATCY	6 cut(s)	46, 295, 705, 772, 1394, 1797
PvuI	CGATCG	1 cut(s)	32
RsaI	GTAC	3 cut(s)	699, 1248, 1321
RsaNI	GTAC	3 cut(s)	698, 1247, 1320
SacI	GAGCTC	1 cut(s)	306
SapI	GCTCTTC	1 cut(s)	2500
SatI	GCNGC	8 cut(s)	53, 184, 208, 414, 509, 938, 1220, 1624
Sau96I	GGNCC	6 cut(s)	153, 209, 1019, 1756, 2264, 2777
SchI	GAGTC	2 cut(s)	2534, 2590
ScrFI	CCNGG	6 cut(s)	171, 204, 212, 213, 987, 2772
SduI	GDGCHC	4 cut(s)	306, 752, 1574, 2378
SfaNI	GCATC	8 cut(s)	445, 586, 881, 1420, 1442, 1481, 1784, 2554
SfoI	GGCGCC	1 cut(s)	1675
SfuI	TTCGAA	5 cut(s)	724, 1147, 1372, 1550, 2315
SinI	GGWCC	3 cut(s)	1019, 1756, 2264
SmaI	CCCGGG	1 cut(s)	213
SmlI	CTYRAG	3 cut(s)	1033, 2152, 2598
SmoI	CTYRAG	3 cut(s)	1033, 2152, 2598
SphI	GCATGC	1 cut(s)	2119
SseBI	AGGCCT	1 cut(s)	74
SspDI	GGCGCC	1 cut(s)	1673
SspI	AATATT	1 cut(s)	1264
SspMI	CTAG	8 cut(s)	675, 942, 1097, 1113, 1863, 1920, 1965, 2142
SstI	GAGCTC	1 cut(s)	306
StuI	AGGCCT	1 cut(s)	74
StyD4I	CCNGG	6 cut(s)	169, 202, 210, 211, 985, 2770
StyI	CCWWGG	7 cut(s)	804, 1271, 1448, 2298, 2389, 2627, 2828
TaaI	ACNGT	6 cut(s)	855, 1213, 1324, 1750, 1984, 2455
TaiI	ACGT	6 cut(s)	12, 498, 1028, 1154, 1252, 1957
TauI	GCSGC	3 cut(s)	210, 940, 1626
TscAI	CASTG	9 cut(s)	148, 384, 392, 752, 887, 1054, 1989, 2385, 2460
TseFI	GTSAC	3 cut(s)	143, 263, 2026
TseI	GCWGC	5 cut(s)	52, 183, 413, 508, 1219
Tsp45I	GTSAC	3 cut(s)	143, 263, 2026
TspDTI	ATGAA	9 cut(s)	89, 618, 929, 1091, 1839, 1965, 2241, 2367, 2733
TspGWI	ACGGA	2 cut(s)	583, 2363
TspMI	CCCGGG	1 cut(s)	211
TspRI	CASTG	9 cut(s)	148, 384, 392, 752, 887, 1054, 1989, 2385, 2460
Van91I	CCANNNNNTGG	2 cut(s)	1501, 1984
Vha464I	CTTAAG	1 cut(s)	2152
VpaK11BI	GGWCC	3 cut(s)	1019, 1756, 2264
XapI	RAATTY	3 cut(s)	442, 1834, 1857
XbaI	TCTAGA	1 cut(s)	1919
XceI	RCATGY	2 cut(s)	1237, 2119
XmaI	CCCGGG	1 cut(s)	211
XmnI	GAANNNNTTC	1 cut(s)	1368
XspI	CTAG	8 cut(s)	675, 942, 1097, 1113, 1863, 1920, 1965, 2142
Zsp2I	ATGCAT	3 cut(s)	636, 1435, 2121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.