FvH4_1g02720

Beta-1,4-mannosyl-glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003347
Physical Location & Seq
Reverse (-)
1554005 .. 1556236
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02720.t1

Sequence Viewer

Length: 1266 bp
ATGCGTTTTGTAAATTGGGGTATAGGCACCACGTCCCGTTATGGATTCCGCGGTTTCTTTAATCAAGGCTTGATGGCAGCACCACCCGGCTATACTTTCAAAAAGAAAAGAAATATCCTTCACGGCATGGCACCACGATCTCTTCGTCCTATTTCCAGACGAACTCCACGCAACTTCTTTTATCTCCTACTGTTGATATTTGTGTCTGCATGTATGTTAGGATTCTATACAGATTACCGGAAAGTTACATACTTCTTGCGGCCACTTTGGGATACGCCGCCAGCTCCGTTCACATGGCTGCCACACTACTATGCAGAAAATGCAAGCATGGAACATCTCTGCCGCCTTCATGGCTGGTCCGTGCGTGCCACCCCACGCCGTATTTTCGATGCCATCATCTTCAGCAATGAGCTAGACTTGCTAGATATCAGGTTGCATGAGCTTTATCCATACGTCACAAAATTTGTAATCATTGAGGGAAATACTACTTTCACTGGAATCCCAAAACCTTGCTTCTTTACTTCAAATCGGAACAGGTTTGCCTTTGCTGAGGAAAAAGTTGTACACCATGTCTTTCCTGGCACAGCTCGAAGTGGAGGTTCACATGCAAACCCCTTCAAATATGAGAGAATGCAACGTGCAGCTATGGATGTGTTACTTCGACAGGCAGGGATTTCCTATGGCGATATCCTGATAATGGCGGATACTGATGAGATCCCTAGCACGAAGGTTTTGAAACTGTTGCAATGGTGTGAGGGAATCCCTTCTGAATTGCATCTTGAGATGAAGCATTACCTGTACTCATTTGAGTTCCCCATGGACAGCATCTGGAAGACTGCAGCCACTATCTACAGCAAGGATACCAGGTACACGCATTCGAGGCGTACTGATGACCTGCTCTCTGATGCAGGATGGCATTGTAGTTTTTGCTTTCGGCACATTGAGGAGTTTGTGTTCAAGATGAGTGCTTATAGCCACGCAGACCGTGTGTGGCGGAGAGATTTTCTGGATTACGGAAGAATACAGAAGCTCATTTGTCAAGGAGATGATCTTTTCGATATGTTGCCTGAAGGGTACACATTCAAGGAAGTGATCAGCAAGATGGGATCGATTCCCAAATCAGCTTCTGCTATTAACCTTCCAGCTTATCTGATAAAGAATGCGGACAAATTTAGGTTCCTTCTCCCTGGAGGCTGTATCAGAGAGACTGGTAGCTCTTCTAACTTTGCTAAGGAAAATAGTCATTCTGATCTTAGTGTTCTTTAG

Protein Analysis

422

Amino Acids

48.8

Weight (kDa)

9.23

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 58 - 402 6.7e-165 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 903
AccB1I GGYRCC 2 cut(s) 26, 130
AccII CGCG 1 cut(s) 51
AciI CCGC 8 cut(s) 49, 51, 259, 278, 343, 701, 994, 1163
AclWI GGATC 2 cut(s) 709, 1114
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 2 cut(s) 461, 1169
AcuI CTGAAG 2 cut(s) 385, 1089
AfaI GTAC 5 cut(s) 564, 800, 869, 886, 1076
AfiI CCNNNNNNNGG 1 cut(s) 697
AgsI TTSAA 6 cut(s) 100, 525, 619, 736, 958, 1084
AjiI CACGTC 1 cut(s) 33
AjnI CCWGG 3 cut(s) 577, 863, 1186
AloI GAACNNNNNNTCC 2 cut(s) 938, 970
AluBI AGCT 9 cut(s) 284, 412, 442, 587, 644, 1030, 1124, 1145, 1215
AluI AGCT 9 cut(s) 284, 412, 442, 587, 644, 1030, 1124, 1145, 1215
Alw26I GTCTC 1 cut(s) 1199
AlwI GGATC 2 cut(s) 709, 1114
AlwNI CAGNNNCTG 2 cut(s) 828, 1127
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 4 cut(s) 77, 298, 641, 839
ApoI RAATTY 2 cut(s) 461, 1169
Asp700I GAANNNNTTC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 87
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 2 cut(s) 26, 130
BbsI GAAGAC 1 cut(s) 839
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 89, 285, 653, 851
BccI CCATC 4 cut(s) 67, 401, 906, 1096
BceAI ACGGC 2 cut(s) 139, 363
BciT130I CCWGG 3 cut(s) 579, 865, 1188
BciVI GTATCC 3 cut(s) 265, 697, 853
BclI TGATCA 1 cut(s) 1092
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 1199
BfaI CTAG 3 cut(s) 413, 422, 720
BfmI CTRYAG 2 cut(s) 837, 850
BfuAI ACCTGC 1 cut(s) 903
BfuI GTATCC 3 cut(s) 265, 697, 853
BglI GCCNNNNNGGC 1 cut(s) 351
BisI GCNGC 7 cut(s) 78, 260, 278, 299, 343, 642, 840
BlsI GCNGC 7 cut(s) 79, 261, 279, 300, 344, 643, 841
Bme1390I CCNGG 4 cut(s) 87, 579, 865, 1188
Bme18I GGWCC 1 cut(s) 357
BmgBI CACGTC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 3 cut(s) 28, 132, 1178
BmrFI CCNGG 4 cut(s) 87, 579, 865, 1188
BmsI GCATC 4 cut(s) 379, 784, 834, 895
BpiI GAAGAC 1 cut(s) 839
BpmI CTGGAG 1 cut(s) 1209
Bpu10I CCTNAGC 2 cut(s) 549, 1230
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 1109
BsaJI CCNNGG 3 cut(s) 49, 816, 1186
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 938, 968
Bsc4I CCNNNNNNNGG 1 cut(s) 697
Bse1I ACTGG 2 cut(s) 499, 1213
Bse3DI GCAATG 2 cut(s) 412, 752
BseBI CCWGG 3 cut(s) 579, 865, 1188
BseCI ATCGAT 1 cut(s) 1109
BseDI CCNNGG 3 cut(s) 49, 816, 1186
BseGI GGATG 2 cut(s) 655, 917
BseLI CCNNNNNNNGG 1 cut(s) 697
BseMI GCAATG 2 cut(s) 412, 752
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 499, 1213
BseRI GAGGAG 1 cut(s) 959
BseXI GCAGC 4 cut(s) 89, 285, 653, 851
BsgI GTGCAG 1 cut(s) 660
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 262
BshNI GGYRCC 2 cut(s) 26, 130
BshVI ATCGAT 1 cut(s) 1109
BsiSI CCGG 2 cut(s) 87, 238
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 697
BsmAI GTCTC 1 cut(s) 1199
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 3 cut(s) 636, 874, 1165
BsnI GGCC 1 cut(s) 262
Bsp1407I TGTACA 1 cut(s) 562
Bsp143I GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 8 cut(s) 49, 51, 259, 278, 343, 701, 994, 1163
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 541
BspDI ATCGAT 1 cut(s) 1109
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 28, 132, 1178
BspMAI CTGCAG 1 cut(s) 841
BspMI ACCTGC 1 cut(s) 903
BspPI GGATC 2 cut(s) 709, 1114
BspQI GCTCTTC 1 cut(s) 1222
BspT107I GGYRCC 2 cut(s) 26, 130
BsrDI GCAATG 2 cut(s) 412, 752
BsrGI TGTACA 1 cut(s) 562
BsrI ACTGG 2 cut(s) 499, 1213
BssECI CCNNGG 3 cut(s) 49, 816, 1186
BssMI GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
BssT1I CCWWGG 1 cut(s) 816
Bst2UI CCWGG 3 cut(s) 579, 865, 1188
Bst4CI ACNGT 3 cut(s) 192, 741, 986
Bst6I CTCTTC 2 cut(s) 147, 1222
BstAPI GCANNNNNTGC 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 562
BstC8I GCNNGC 3 cut(s) 282, 325, 366
BstDEI CTNAG 3 cut(s) 549, 1230, 1253
BstDSI CCRYGG 2 cut(s) 49, 816
BstF5I GGATG 2 cut(s) 655, 917
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 6 cut(s) 140, 717, 1051, 1095, 1109, 1252
BstMAI GTCTC 1 cut(s) 1199
BstMBI GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
BstMWI GCNNNNNNNGC 4 cut(s) 320, 351, 418, 880
BstNI CCWGG 3 cut(s) 579, 865, 1188
BstNSI RCATGY 2 cut(s) 213, 608
BstSCI CCNGG 4 cut(s) 85, 577, 863, 1186
BstSFI CTRYAG 2 cut(s) 837, 850
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 4 cut(s) 89, 285, 653, 851
BstV2I GAAGAC 1 cut(s) 839
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
Bsu15I ATCGAT 1 cut(s) 1109
BsuI GTATCC 3 cut(s) 265, 697, 853
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 1 cut(s) 1109
BtgI CCRYGG 2 cut(s) 49, 816
BtrI CACGTC 1 cut(s) 33
BtsCI GGATG 2 cut(s) 655, 917
BtsIMutI CAGTG 1 cut(s) 492
BveI ACCTGC 1 cut(s) 903
Cac8I GCNNGC 3 cut(s) 282, 325, 366
CaiI CAGNNNCTG 2 cut(s) 828, 1127
Cfr13I GGNCC 1 cut(s) 357
Cfr42I CCGCGG 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 1109
CsiI ACCWGGT 1 cut(s) 863
Csp6I GTAC 5 cut(s) 563, 799, 868, 885, 1075
CviAII CATG 9 cut(s) 127, 210, 294, 328, 350, 437, 569, 605, 817
CviQI GTAC 5 cut(s) 563, 799, 868, 885, 1075
DdeI CTNAG 3 cut(s) 549, 1230, 1253
DpnI GATC 6 cut(s) 139, 716, 1050, 1094, 1108, 1251
DpnII GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
EaeI YGGCCR 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 147, 1222
EarI CTCTTC 2 cut(s) 147, 1222
EciI GGCGGA 2 cut(s) 716, 1009
Eco130I CCWWGG 1 cut(s) 816
Eco32I GATATC 2 cut(s) 427, 688
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 385, 1089
EcoRII CCWGG 3 cut(s) 577, 863, 1186
EcoRV GATATC 2 cut(s) 427, 688
EcoT14I CCWWGG 1 cut(s) 816
ErhI CCWWGG 1 cut(s) 816
FaeI CATG 9 cut(s) 130, 213, 297, 331, 353, 440, 572, 608, 820
FaqI GGGAC 1 cut(s) 19
FatI CATG 9 cut(s) 126, 209, 293, 327, 349, 436, 568, 604, 816
FbaI TGATCA 1 cut(s) 1092
Fnu4HI GCNGC 7 cut(s) 78, 260, 278, 299, 343, 642, 840
FokI GGATG 2 cut(s) 662, 924
Fsp4HI GCNGC 7 cut(s) 78, 260, 278, 299, 343, 642, 840
FspBI CTAG 3 cut(s) 413, 422, 720
GluI GCNGC 7 cut(s) 78, 260, 278, 299, 343, 642, 840
GsuI CTGGAG 1 cut(s) 1209
HaeIII GGCC 1 cut(s) 262
HapII CCGG 2 cut(s) 87, 238
Hin1II CATG 9 cut(s) 130, 213, 297, 331, 353, 440, 572, 608, 820
HinfI GANTC 5 cut(s) 45, 222, 498, 759, 1111
HpaII CCGG 2 cut(s) 87, 238
Hpy166II GTNNAC 5 cut(s) 291, 565, 602, 870, 1077
Hpy188I TCNGA 6 cut(s) 531, 769, 904, 1152, 1202, 1249
Hpy188III TCNNGA 6 cut(s) 156, 691, 779, 829, 958, 1007
Hpy8I GTNNAC 5 cut(s) 291, 565, 602, 870, 1077
HpyAV CCTTC 8 cut(s) 128, 356, 625, 721, 774, 1064, 1148, 1190
HpyCH4III ACNGT 3 cut(s) 192, 741, 986
HpyCH4IV ACGT 3 cut(s) 32, 453, 637
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 351, 418, 880
HpyF3I CTNAG 3 cut(s) 549, 1230, 1253
HpySE526I ACGT 3 cut(s) 32, 453, 637
Hsp92II CATG 9 cut(s) 130, 213, 297, 331, 353, 440, 572, 608, 820
Ksp22I TGATCA 1 cut(s) 1092
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
LguI GCTCTTC 1 cut(s) 1222
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 4 cut(s) 89, 285, 653, 851
LweI GCATC 4 cut(s) 379, 784, 834, 895
MabI ACCWGGT 1 cut(s) 863
MaeI CTAG 3 cut(s) 413, 422, 720
MaeII ACGT 3 cut(s) 32, 453, 637
MaeIII GTNAC 3 cut(s) 244, 454, 654
MalI GATC 6 cut(s) 139, 716, 1050, 1094, 1108, 1251
MboI GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
MboII GAAGA 5 cut(s) 134, 391, 844, 1029, 1209
MflI RGATCY 1 cut(s) 714
MluCI AATT 4 cut(s) 13, 461, 770, 1169
MnlI CCTC 7 cut(s) 469, 544, 590, 748, 873, 937, 1184
MroXI GAANNNNTTC 1 cut(s) 763
MseI TTAA 2 cut(s) 60, 1134
MslI CAYNNNNRTG 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 2 cut(s) 87, 238
MspR9I CCNGG 4 cut(s) 87, 579, 865, 1188
Mva1269I GAATGC 3 cut(s) 636, 874, 1165
MvaI CCWGG 3 cut(s) 579, 865, 1188
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 4 cut(s) 320, 351, 418, 880
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 816
NdeII GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
NlaIII CATG 9 cut(s) 130, 213, 297, 331, 353, 440, 572, 608, 820
NlaIV GGNNCC 3 cut(s) 28, 132, 1178
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 2 cut(s) 213, 608
PciSI GCTCTTC 1 cut(s) 1222
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PctI GAATGC 3 cut(s) 636, 874, 1165
PdmI GAANNNNTTC 1 cut(s) 763
PfeI GAWTC 5 cut(s) 45, 222, 498, 759, 1111
PkrI GCNGC 7 cut(s) 79, 261, 279, 300, 344, 643, 841
Psp6I CCWGG 3 cut(s) 577, 863, 1186
PspGI CCWGG 3 cut(s) 577, 863, 1186
PspN4I GGNNCC 3 cut(s) 28, 132, 1178
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 841
PstNI CAGNNNCTG 2 cut(s) 828, 1127
PsuI RGATCY 1 cut(s) 714
RsaI GTAC 5 cut(s) 564, 800, 869, 886, 1076
RsaNI GTAC 5 cut(s) 563, 799, 868, 885, 1075
RseI CAYNNNNRTG 1 cut(s) 309
SacII CCGCGG 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 1222
SaqAI TTAA 2 cut(s) 60, 1134
SatI GCNGC 7 cut(s) 78, 260, 278, 299, 343, 642, 840
Sau3AI GATC 6 cut(s) 137, 714, 1048, 1092, 1106, 1249
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 4 cut(s) 87, 579, 865, 1188
SexAI ACCWGGT 1 cut(s) 863
SfaNI GCATC 4 cut(s) 379, 784, 834, 895
SfcI CTRYAG 2 cut(s) 837, 850
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 4 cut(s) 13, 461, 770, 1169
SsiI CCGC 8 cut(s) 49, 51, 259, 278, 343, 701, 994, 1163
SspMI CTAG 3 cut(s) 413, 422, 720
StyD4I CCNGG 4 cut(s) 85, 577, 863, 1186
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 3 cut(s) 192, 741, 986
TaiI ACGT 3 cut(s) 35, 456, 640
TaqI TCGA 6 cut(s) 387, 589, 661, 878, 1056, 1109
TasI AATT 4 cut(s) 13, 461, 770, 1169
TatI WGTACW 2 cut(s) 562, 798
TauI GCSGC 3 cut(s) 262, 280, 345
TfiI GAWTC 5 cut(s) 45, 222, 498, 759, 1111
Tru1I TTAA 2 cut(s) 60, 1134
Tru9I TTAA 2 cut(s) 60, 1134
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 4 cut(s) 77, 298, 641, 839
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 338, 800
TspGWI ACGGA 3 cut(s) 276, 349, 1029
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 2 cut(s) 461, 1169
XceI RCATGY 2 cut(s) 213, 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 763
XspI CTAG 3 cut(s) 413, 422, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.