Rh2BG032300

Beta-1,4-mannosyl-glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003347
Physical Location & Seq
Forward (+)
2227914 .. 2231724
3811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG032300.1

Sequence Viewer

Length: 1128 bp
ATGTTAAACACTAAACCTCTCGTAATCGTCCTTCAAGGCATGGCACCACGAACTCTTCCTCTTCGTCCTACTTCCAGACGGACTCCACGCAAGTTCTTTTGTCTGTTACTGTTGATATTTGTGCCTGCATGTATATTTGGACTCTATACTGATCACCGGAAAGTTACATATTTCTTCCGGCCACTTTGGGATAAGCCTCCAGCTCCGTTCATACAGCTGCCACACTACTATGCAGAAAATGTAAGTATGGACCATCTTTGCCGCCTTCATGGCTGGTCTCTGCGTGCCACCCCACGCCGTATTTTTGATGCCATCATCTTCAGCAATGAATTAGACTTGCTTGATATCAGGTGGCATGAACTTTATCCATATGTCACAAAATTTGTAATCATCGAGTCAAATAGTACTTTCACTGGAATCCCAAAACCTCTCTACTTTGCTTCAAATCGGAACAGGTTTGCCTTTGCTGAGGAAAAAATCATCCACTATGCCTTTCCTGGAAGAACTCTACGCCGAGGTTCAAATGCAAACCCCTTCAAACTTGAGAGAAAGCAACGTGTAGCTATGAATGCTTTACTTCAGTATGCAGGGATTTCCTATGGCGATGTCGTGATAATGTCAGATACTGATGAGATCCCTAGCTCGCAGACTTTGAAACTATTGCAATGGTGTGATGGGATCCCTTCTAAAATGCATCTTGAGCTGAAGCACTACATGTACTCATTTGAGTTCCCGGTGGACTTTAGCAGCTGGCGGGCTACAGCTCAGATCTACAGCCCGAATACCAAATACCAGCATACCCGGCAAACTGATGACATGCTCTCTGATGCGGGATGGCATTGTAGTTTTTGCTTTCGGCACATTGATGACTTTGTGTTCAAAATGACTGCTTATAGCCACGCAGACCGTGTGAGGCGGAGAGATTTTCTGGATTATAGAAGAATACAGAGGCTCATTTGTCAAGGAGACGATCTTTTCGATATGTTGCCTGAAGAGTACACATTCAAGGACTTGATTAAGAAGATGGGATCGATTCCCAAATCAGCTTCTGCAGTTCATCTTCCTGCTTATTTGATAAAGAATGCAGACAAGTTTAGGTTTCTTCTCCCTGGAGGCTGTATCAGATAA

Protein Analysis

375

Amino Acids

44.04

Weight (kDa)

9.43

Isoelectric Point (pI)

55.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 31 - 375 4.3e-178 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 43
AciI CCGC 4 cut(s) 262, 754, 830, 916
AclWI GGATC 4 cut(s) 628, 673, 686, 1036
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 4 cut(s) 304, 563, 725, 1011
AfaI GTAC 3 cut(s) 406, 719, 998
AflIII ACRYGT 2 cut(s) 556, 714
AgsI TTSAA 7 cut(s) 35, 444, 522, 538, 655, 880, 1006
AjnI CCWGG 2 cut(s) 496, 1108
AluBI AGCT 8 cut(s) 203, 217, 563, 642, 703, 750, 764, 1046
AluI AGCT 8 cut(s) 203, 217, 563, 642, 703, 750, 764, 1046
Alw26I GTCTC 2 cut(s) 282, 960
AlwI GGATC 4 cut(s) 628, 673, 686, 1036
AlwNI CAGNNNCTG 2 cut(s) 626, 1049
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 2 cut(s) 217, 747
ApoI RAATTY 1 cut(s) 380
ArsI GACNNNNNNTTYG 2 cut(s) 959, 991
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 2 cut(s) 734, 802
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 250
BamHI GGATCC 1 cut(s) 678
BanI GGYRCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BbvCI CCTCAGC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 204, 759
BccI CCATC 5 cut(s) 261, 320, 668, 828, 1018
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 2 cut(s) 498, 1110
BclI TGATCA 1 cut(s) 151
BcnI CCSGG 2 cut(s) 734, 802
BcoDI GTCTC 2 cut(s) 282, 960
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 3 cut(s) 759, 772, 1050
BglI GCCNNNNNGGC 1 cut(s) 270
BglII AGATCT 1 cut(s) 768
BisI GCNGC 3 cut(s) 218, 262, 748
BlsI GCNGC 3 cut(s) 219, 263, 749
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 4 cut(s) 498, 734, 802, 1110
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 2 cut(s) 45, 680
BmrFI CCNGG 4 cut(s) 498, 734, 802, 1110
BmsI GCATC 3 cut(s) 298, 703, 817
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 468
BpuEI CTTGAG 2 cut(s) 563, 719
BpuMI CCSGG 2 cut(s) 734, 802
Bsa29I ATCGAT 1 cut(s) 1031
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 514, 1108
BsaWI WCCGGW 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 2 cut(s) 331, 671
BseBI CCWGG 2 cut(s) 498, 1110
BseCI ATCGAT 1 cut(s) 1031
BseDI CCNNGG 2 cut(s) 514, 1108
BseGI GGATG 2 cut(s) 480, 839
BseMI GCAATG 2 cut(s) 331, 671
BseMII CTCAG 2 cut(s) 459, 779
BseNI ACTGG 1 cut(s) 418
BseXI GCAGC 2 cut(s) 204, 759
BshFI GGCC 1 cut(s) 181
BshNI GGYRCC 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 1031
BsiSI CCGG 4 cut(s) 157, 178, 734, 802
BsmAI GTCTC 2 cut(s) 282, 960
BsmBI CGTCTC 1 cut(s) 960
BsmI GAATGC 2 cut(s) 574, 1087
BsnI GGCC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 282
Bsp143I GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
BspACI CCGC 4 cut(s) 262, 754, 830, 916
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 2 cut(s) 460, 778
BspDI ATCGAT 1 cut(s) 1031
BspLI GGNNCC 2 cut(s) 45, 680
BspMAI CTGCAG 1 cut(s) 1054
BspPI GGATC 4 cut(s) 628, 673, 686, 1036
BspT107I GGYRCC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 282
BsrDI GCAATG 2 cut(s) 331, 671
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 514, 1108
BssMI GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
Bst2UI CCWGG 2 cut(s) 498, 1110
Bst4CI ACNGT 2 cut(s) 111, 908
Bst6I CTCTTC 3 cut(s) 60, 66, 987
BstC8I GCNNGC 5 cut(s) 126, 285, 644, 752, 756
BstDEI CTNAG 2 cut(s) 468, 765
BstF5I GGATG 2 cut(s) 480, 839
BstKTI GATC 6 cut(s) 154, 636, 681, 771, 973, 1031
BstMAI GTCTC 2 cut(s) 282, 960
BstMBI GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
BstMWI GCNNNNNNNGC 4 cut(s) 270, 569, 700, 802
BstNI CCWGG 2 cut(s) 498, 1110
BstNSI RCATGY 3 cut(s) 132, 718, 820
BstSCI CCNGG 4 cut(s) 496, 732, 800, 1108
BstSFI CTRYAG 3 cut(s) 759, 772, 1050
BstV1I GCAGC 2 cut(s) 204, 759
BstX2I RGATCY 3 cut(s) 633, 678, 768
BstYI RGATCY 3 cut(s) 633, 678, 768
Bsu15I ATCGAT 1 cut(s) 1031
BsuRI GGCC 1 cut(s) 181
BsuTUI ATCGAT 1 cut(s) 1031
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 2 cut(s) 480, 839
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 5 cut(s) 126, 285, 644, 752, 756
CaiI CAGNNNCTG 2 cut(s) 626, 1049
Cfr13I GGNCC 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 1031
Csp6I GTAC 3 cut(s) 405, 718, 997
CviAII CATG 6 cut(s) 40, 129, 269, 356, 715, 817
CviQI GTAC 3 cut(s) 405, 718, 997
DdeI CTNAG 2 cut(s) 468, 765
DpnI GATC 6 cut(s) 153, 635, 680, 770, 972, 1030
DpnII GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
EaeI YGGCCR 1 cut(s) 179
Eam1104I CTCTTC 3 cut(s) 60, 66, 987
EarI CTCTTC 3 cut(s) 60, 66, 987
EciI GGCGGA 1 cut(s) 931
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 250
Eco57I CTGAAG 4 cut(s) 304, 563, 725, 1011
EcoRII CCWGG 2 cut(s) 496, 1108
EcoRV GATATC 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 696
Esp3I CGTCTC 1 cut(s) 960
FaeI CATG 6 cut(s) 43, 132, 272, 359, 718, 820
FatI CATG 6 cut(s) 39, 128, 268, 355, 714, 816
FauI CCCGC 2 cut(s) 747, 823
FauNDI CATATG 1 cut(s) 370
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 218, 262, 748
FokI GGATG 2 cut(s) 467, 846
Fsp4HI GCNGC 3 cut(s) 218, 262, 748
FspBI CTAG 1 cut(s) 639
GluI GCNGC 3 cut(s) 218, 262, 748
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 181
HapII CCGG 4 cut(s) 157, 178, 734, 802
Hin1II CATG 6 cut(s) 43, 132, 272, 359, 718, 820
HinfI GANTC 5 cut(s) 82, 141, 395, 417, 1033
HpaII CCGG 4 cut(s) 157, 178, 734, 802
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 739, 999
Hpy188I TCNGA 5 cut(s) 450, 622, 768, 826, 1124
Hpy188III TCNNGA 4 cut(s) 75, 610, 698, 929
Hpy8I GTNNAC 2 cut(s) 739, 999
HpyAV CCTTC 4 cut(s) 41, 275, 544, 693
HpyCH4III ACNGT 2 cut(s) 111, 908
HpyCH4IV ACGT 1 cut(s) 556
HpyCH4V TGCA 8 cut(s) 128, 233, 527, 587, 664, 694, 1052, 1085
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 569, 700, 802
HpyF3I CTNAG 2 cut(s) 468, 765
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 6 cut(s) 43, 132, 272, 359, 718, 820
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 2 cut(s) 204, 759
LweI GCATC 3 cut(s) 298, 703, 817
MaeI CTAG 1 cut(s) 639
MaeII ACGT 1 cut(s) 556
MaeIII GTNAC 3 cut(s) 105, 163, 373
MalI GATC 6 cut(s) 153, 635, 680, 770, 972, 1030
MboI GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
MflI RGATCY 3 cut(s) 633, 678, 768
MluCI AATT 2 cut(s) 329, 380
MlyI GAGTC 3 cut(s) 76, 135, 404
MnlI CCTC 9 cut(s) 27, 69, 207, 438, 463, 509, 906, 942, 1106
Mph1103I ATGCAT 1 cut(s) 696
MseI TTAA 2 cut(s) 5, 1017
MslI CAYNNNNRTG 2 cut(s) 228, 864
MspA1I CMGCKG 2 cut(s) 217, 750
MspI CCGG 4 cut(s) 157, 178, 734, 802
MspR9I CCNGG 4 cut(s) 498, 734, 802, 1110
Mva1269I GAATGC 2 cut(s) 574, 1087
MvaI CCWGG 2 cut(s) 498, 1110
MwoI GCNNNNNNNGC 4 cut(s) 270, 569, 700, 802
NciI CCSGG 2 cut(s) 734, 802
NdeI CATATG 1 cut(s) 370
NdeII GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
NlaIII CATG 6 cut(s) 43, 132, 272, 359, 718, 820
NlaIV GGNNCC 2 cut(s) 45, 680
NmeAIII GCCGAG 1 cut(s) 539
NmuCI GTSAC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 696
NspI RCATGY 3 cut(s) 132, 718, 820
PciI ACATGT 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 975
PctI GAATGC 2 cut(s) 574, 1087
PfeI GAWTC 2 cut(s) 417, 1033
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 3 cut(s) 219, 263, 749
PleI GAGTC 3 cut(s) 76, 135, 403
PpsI GAGTC 3 cut(s) 76, 135, 403
PscI ACATGT 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 496, 1108
PspGI CCWGG 2 cut(s) 496, 1108
PspN4I GGNNCC 2 cut(s) 45, 680
PspPI GGNCC 1 cut(s) 250
PstI CTGCAG 1 cut(s) 1054
PstNI CAGNNNCTG 2 cut(s) 626, 1049
PsuI RGATCY 3 cut(s) 633, 678, 768
PvuII CAGCTG 2 cut(s) 217, 750
RsaI GTAC 3 cut(s) 406, 719, 998
RsaNI GTAC 3 cut(s) 405, 718, 997
RseI CAYNNNNRTG 2 cut(s) 228, 864
SaqAI TTAA 2 cut(s) 5, 1017
SatI GCNGC 3 cut(s) 218, 262, 748
Sau3AI GATC 6 cut(s) 151, 633, 678, 768, 970, 1028
Sau96I GGNCC 1 cut(s) 250
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 3 cut(s) 76, 135, 404
ScrFI CCNGG 4 cut(s) 498, 734, 802, 1110
SfaNI GCATC 3 cut(s) 298, 703, 817
SfcI CTRYAG 3 cut(s) 759, 772, 1050
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 228, 864
SmlI CTYRAG 2 cut(s) 542, 698
SmoI CTYRAG 2 cut(s) 542, 698
Sse9I AATT 2 cut(s) 329, 380
SsiI CCGC 4 cut(s) 262, 754, 830, 916
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 4 cut(s) 496, 732, 800, 1108
TaaI ACNGT 2 cut(s) 111, 908
TaiI ACGT 1 cut(s) 559
TaqI TCGA 3 cut(s) 393, 978, 1031
TasI AATT 2 cut(s) 329, 380
TatI WGTACW 3 cut(s) 404, 717, 996
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 2 cut(s) 417, 1033
Tru1I TTAA 2 cut(s) 5, 1017
Tru9I TTAA 2 cut(s) 5, 1017
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 2 cut(s) 217, 747
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 6 cut(s) 199, 257, 342, 372, 581, 1046
TspGWI ACGGA 2 cut(s) 94, 195
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 380
XceI RCATGY 3 cut(s) 132, 718, 820
XspI CTAG 1 cut(s) 639
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.