Rh2CG033300

Beta-1,4-mannosyl-glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003347
Physical Location & Seq
Reverse (-)
2580744 .. 2592207
11464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG033300.1

Sequence Viewer

Length: 1314 bp
ATGCATGCGCCTGCACTGATTAAGGTGAGGCCTCCTTGGAACCCCCAATTTTGTGGTGGTAGCAAAATTCCTGGTGAAGGGCTATCTCAGTTCCTTTTTATCAGAAAAGCATGGTGGGGTTCTTCAAGAATCTCACTTTCAGTCACTTTGAGTAATGATAAAGCAAACAAGGGCATGGCACCACGATCTCTTCGTCATACTCCCAGACGGACTCCACGCAAGTTCTTTTATCTCTTACTGTTGATATTCGTGCCTGCATGTATATTTGGACTCTTTATAGATTACCGGAAAGTTACATATTTCCTCCGGCCACTTTGGGATACGCCTCCAGCTCCGTTCACATGGCTGCCACACTACTATGCAGAAAATGTAAGCATGGACCATCTCTGCCGCCTTCATGGCTGGTCCGTGCGTGCCACTCCACGCCGTATTTTTGATGCCATCATCTTCAGCAATGAAATAGACTTGCTTGATATCAGGTGGCATGAACTTTATCCATATGTCACAAAATTTGTAATCATCGAGGCAAATACTACTTTCACTGGAATCCCTAAACCTCTCTACTTTGCTTCAAATCGGAACAGATTTGCCTTTGCTGATGAAAAAATTGTGCACTATGTCTTTCCTGGCACAACTCGAAGCGGAGATTCACATGCAGACCCCTTCAAATTTGAGAGAATGCAACGTGCAGCTATGGATGATTTACTTCGGCAGGCAGGGATTTCCAATGGCGATGTCCTGATAATGGCGGATACTGATGAGATCCCTAGGCCGCAGACGTTGAAACTGTTGCAATGGTGTGGTGGGATCCCTTCTAAACTGCATTTTGAGATGAAGCACTACCTGTACTCATTTGAGTTCCCAATGGACAGCATCTGGAAGACTGCATCTAATATCTACGGCCCGAATACCAGGTACATGCATTCGAGGCAAACTGATGACCTGCTCTCTGATGCGGGATGGCATTGTAGTTTTTGCTTTCGGCACATTGAGGAGTTTGTGTTCAAGATGGGAGCTTATAGCCACGCAGAGCGTGTGAGGCGGAGAGACTTTCTGGATTACAGAAGAATACAGAGGCTCATTTGTCATGGTGACGATCTTTTCGATATGTTACCTGAAGAGTTCACATTCAAGGACTTGATCAGGAAGATGGGATCGATTCCCAAATCAGCTTCTGCAATTACCCTTCCTGCTTATTTGATAAAGAATGCGGACAAGTTTAGGTACCTTCTCCCTGGAGGCTGTATCAGAGAGACCGGTAGCTCTTCTAATTTTCCTAAGGAAAGTAGTTATTCTGATCCTAGTGTTTTTTAG

Protein Analysis

437

Amino Acids

50.81

Weight (kDa)

9.3

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 74 - 418 1.4e-166 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 951
Acc65I GGTACC 1 cut(s) 1224
AccB1I GGYRCC 2 cut(s) 178, 1224
AciI CCGC 7 cut(s) 391, 642, 749, 773, 956, 1042, 1211
AclWI GGATC 5 cut(s) 757, 802, 815, 1162, 1292
AcoI YGGCCR 1 cut(s) 308
AcsI RAATTY 3 cut(s) 66, 509, 668
AcuI CTGAAG 2 cut(s) 433, 1137
AfaI GTAC 3 cut(s) 848, 917, 1226
AfiI CCNNNNNNNGG 2 cut(s) 77, 745
AgeI ACCGGT 1 cut(s) 1256
AgsI TTSAA 6 cut(s) 126, 573, 667, 784, 1006, 1132
AjnI CCWGG 4 cut(s) 70, 625, 911, 1234
AloI GAACNNNNNNTCC 2 cut(s) 986, 1018
AluBI AGCT 5 cut(s) 332, 692, 1016, 1172, 1263
AluI AGCT 5 cut(s) 332, 692, 1016, 1172, 1263
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 2 cut(s) 1041, 1247
Alw44I GTGCAC 1 cut(s) 611
AlwI GGATC 5 cut(s) 757, 802, 815, 1162, 1292
AlwNI CAGNNNCTG 2 cut(s) 876, 1175
AoxI GGCC 4 cut(s) 29, 308, 770, 901
ApaLI GTGCAC 1 cut(s) 611
ApeKI GCWGC 2 cut(s) 346, 689
ApoI RAATTY 3 cut(s) 66, 509, 668
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
AsiGI ACCGGT 1 cut(s) 1256
Asp718I GGTACC 1 cut(s) 1224
AspA2I CCTAGG 1 cut(s) 767
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 3 cut(s) 379, 405, 902
AsuHPI GGTGA 3 cut(s) 37, 86, 1103
AvaII GGWCC 2 cut(s) 379, 405
AvrII CCTAGG 1 cut(s) 767
AxyI CCTNAGG 1 cut(s) 1278
BaeGI GKGCMC 1 cut(s) 615
BamHI GGATCC 1 cut(s) 807
BanI GGYRCC 2 cut(s) 178, 1224
BbsI GAAGAC 1 cut(s) 887
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 2 cut(s) 333, 701
BccI CCATC 5 cut(s) 390, 449, 954, 1003, 1144
BceAI ACGGC 2 cut(s) 411, 916
BciT130I CCWGG 4 cut(s) 72, 627, 913, 1236
BciVI GTATCC 2 cut(s) 313, 745
BclI TGATCA 1 cut(s) 1140
BcoDI GTCTC 2 cut(s) 1041, 1247
BfaI CTAG 2 cut(s) 768, 1302
BfuAI ACCTGC 1 cut(s) 951
BfuI GTATCC 2 cut(s) 313, 745
BglI GCCNNNNNGGC 1 cut(s) 399
BisI GCNGC 4 cut(s) 347, 391, 690, 773
BlnI CCTAGG 1 cut(s) 767
BlsI GCNGC 4 cut(s) 348, 392, 691, 774
Bme1390I CCNGG 4 cut(s) 72, 627, 913, 1236
Bme18I GGWCC 2 cut(s) 379, 405
BmgT120I GGNCC 3 cut(s) 379, 405, 902
BmiI GGNNCC 4 cut(s) 41, 180, 809, 1226
BmrFI CCNGG 4 cut(s) 72, 627, 913, 1236
BmsI GCATC 4 cut(s) 427, 882, 896, 943
BpiI GAAGAC 1 cut(s) 887
BpmI CTGGAG 2 cut(s) 312, 1257
Bsa29I ATCGAT 1 cut(s) 1157
BsaI GGTCTC 1 cut(s) 1247
BsaJI CCNNGG 3 cut(s) 35, 767, 1234
BsaWI WCCGGW 2 cut(s) 285, 1256
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 745
Bse118I RCCGGY 1 cut(s) 1256
Bse1I ACTGG 1 cut(s) 547
Bse21I CCTNAGG 1 cut(s) 1278
Bse3DI GCAATG 2 cut(s) 460, 800
BseBI CCWGG 4 cut(s) 72, 627, 913, 1236
BseCI ATCGAT 1 cut(s) 1157
BseDI CCNNGG 3 cut(s) 35, 767, 1234
BseGI GGATG 2 cut(s) 703, 965
BseLI CCNNNNNNNGG 2 cut(s) 77, 745
BseMI GCAATG 2 cut(s) 460, 800
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 1007
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 2 cut(s) 333, 701
BsgI GTGCAG 1 cut(s) 708
BshFI GGCC 4 cut(s) 31, 310, 772, 903
BshNI GGYRCC 2 cut(s) 178, 1224
BshTI ACCGGT 1 cut(s) 1256
BshVI ATCGAT 1 cut(s) 1157
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 3 cut(s) 286, 307, 1257
BslI CCNNNNNNNGG 2 cut(s) 77, 745
BsmAI GTCTC 2 cut(s) 1041, 1247
BsmI GAATGC 3 cut(s) 684, 922, 1213
BsnI GGCC 4 cut(s) 31, 310, 772, 903
Bso31I GGTCTC 1 cut(s) 1247
Bsp1286I GDGCHC 1 cut(s) 615
Bsp143I GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
BspACI CCGC 7 cut(s) 391, 642, 749, 773, 956, 1042, 1211
BspANI GGCC 4 cut(s) 31, 310, 772, 903
BspCNI CTCAG 1 cut(s) 100
BspDI ATCGAT 1 cut(s) 1157
BspLI GGNNCC 4 cut(s) 41, 180, 809, 1226
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 5 cut(s) 757, 802, 815, 1162, 1292
BspQI GCTCTTC 1 cut(s) 1270
BspT107I GGYRCC 2 cut(s) 178, 1224
BspTNI GGTCTC 1 cut(s) 1247
BsrDI GCAATG 2 cut(s) 460, 800
BsrFI RCCGGY 1 cut(s) 1256
BsrI ACTGG 1 cut(s) 547
BssAI RCCGGY 1 cut(s) 1256
BssECI CCNNGG 3 cut(s) 35, 767, 1234
BssMI GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
BssT1I CCWWGG 2 cut(s) 35, 767
Bst2UI CCWGG 4 cut(s) 72, 627, 913, 1236
Bst4CI ACNGT 2 cut(s) 240, 789
Bst6I CTCTTC 3 cut(s) 195, 1113, 1270
BstC8I GCNNGC 5 cut(s) 6, 12, 255, 414, 714
BstDEI CTNAG 2 cut(s) 87, 1278
BstENI CCTNNNNNAGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 703, 965
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 7 cut(s) 188, 765, 810, 1099, 1143, 1157, 1300
BstMAI GTCTC 2 cut(s) 1041, 1247
BstMBI GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
BstMWI GCNNNNNNNGC 3 cut(s) 399, 928, 1039
BstNI CCWGG 4 cut(s) 72, 627, 913, 1236
BstNSI RCATGY 4 cut(s) 8, 261, 656, 922
BstSCI CCNGG 4 cut(s) 70, 625, 911, 1234
BstSLI GKGCMC 1 cut(s) 615
BstV1I GCAGC 2 cut(s) 333, 701
BstV2I GAAGAC 1 cut(s) 887
BstX2I RGATCY 2 cut(s) 762, 807
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 2 cut(s) 762, 807
Bsu15I ATCGAT 1 cut(s) 1157
Bsu36I CCTNAGG 1 cut(s) 1278
BsuI GTATCC 2 cut(s) 313, 745
BsuRI GGCC 4 cut(s) 31, 310, 772, 903
BsuTUI ATCGAT 1 cut(s) 1157
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 2 cut(s) 703, 965
BtsIMutI CAGTG 2 cut(s) 14, 540
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 5 cut(s) 6, 12, 255, 414, 714
CaiI CAGNNNCTG 2 cut(s) 876, 1175
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 1256
Cfr13I GGNCC 3 cut(s) 379, 405, 902
ClaI ATCGAT 1 cut(s) 1157
CsiI ACCWGGT 1 cut(s) 911
Csp6I GTAC 3 cut(s) 847, 916, 1225
CspAI ACCGGT 1 cut(s) 1256
CviQI GTAC 3 cut(s) 847, 916, 1225
DdeI CTNAG 2 cut(s) 87, 1278
DpnI GATC 7 cut(s) 187, 764, 809, 1098, 1142, 1156, 1299
DpnII GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 3 cut(s) 195, 1113, 1270
EarI CTCTTC 3 cut(s) 195, 1113, 1270
EciI GGCGGA 2 cut(s) 764, 1057
Eco130I CCWWGG 2 cut(s) 35, 767
Eco147I AGGCCT 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 1247
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 379, 405
Eco57I CTGAAG 2 cut(s) 433, 1137
Eco81I CCTNAGG 1 cut(s) 1278
EcoNI CCTNNNNNAGG 1 cut(s) 75
EcoRII CCWGG 4 cut(s) 70, 625, 911, 1234
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 35, 767
EcoT22I ATGCAT 2 cut(s) 6, 924
ErhI CCWWGG 2 cut(s) 35, 767
FauI CCCGC 1 cut(s) 949
FauNDI CATATG 1 cut(s) 499
FbaI TGATCA 1 cut(s) 1140
Fnu4HI GCNGC 4 cut(s) 347, 391, 690, 773
FokI GGATG 2 cut(s) 710, 972
Fsp4HI GCNGC 4 cut(s) 347, 391, 690, 773
FspBI CTAG 2 cut(s) 768, 1302
GlaI GCGC 1 cut(s) 9
GluI GCNGC 4 cut(s) 347, 391, 690, 773
GsuI CTGGAG 2 cut(s) 312, 1257
HaeIII GGCC 4 cut(s) 31, 310, 772, 903
HapII CCGG 3 cut(s) 286, 307, 1257
HhaI GCGC 1 cut(s) 10
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 6 cut(s) 129, 211, 270, 546, 647, 1159
HpaII CCGG 3 cut(s) 286, 307, 1257
HphI GGTGA 3 cut(s) 37, 86, 1103
Hpy166II GTNNAC 3 cut(s) 339, 613, 1125
Hpy188I TCNGA 5 cut(s) 104, 579, 952, 1250, 1297
Hpy188III TCNNGA 6 cut(s) 126, 739, 877, 1006, 1055, 1144
Hpy8I GTNNAC 3 cut(s) 339, 613, 1125
HpyAV CCTTC 6 cut(s) 71, 404, 673, 822, 1196, 1238
HpyCH4III ACNGT 2 cut(s) 240, 789
HpyCH4IV ACGT 2 cut(s) 685, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 399, 928, 1039
HpyF3I CTNAG 2 cut(s) 87, 1278
HpySE526I ACGT 2 cut(s) 685, 779
HspAI GCGC 1 cut(s) 8
KpnI GGTACC 1 cut(s) 1228
Ksp22I TGATCA 1 cut(s) 1140
Kzo9I GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
LguI GCTCTTC 1 cut(s) 1270
LmnI GCTCC 2 cut(s) 337, 1013
Lsp1109I GCAGC 2 cut(s) 333, 701
LweI GCATC 4 cut(s) 427, 882, 896, 943
MabI ACCWGGT 1 cut(s) 911
MaeI CTAG 2 cut(s) 768, 1302
MaeII ACGT 2 cut(s) 685, 779
MaeIII GTNAC 5 cut(s) 142, 292, 502, 1091, 1110
MalI GATC 7 cut(s) 187, 764, 809, 1098, 1142, 1156, 1299
MboI GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
MboII GAAGA 8 cut(s) 114, 182, 439, 892, 1077, 1130, 1159, 1257
MflI RGATCY 2 cut(s) 762, 807
MhlI GDGCHC 1 cut(s) 615
MluCI AATT 7 cut(s) 47, 66, 509, 606, 668, 1179, 1270
MlyI GAGTC 2 cut(s) 205, 264
Mph1103I ATGCAT 2 cut(s) 6, 924
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 3 cut(s) 286, 307, 1257
MspR9I CCNGG 4 cut(s) 72, 627, 913, 1236
Mva1269I GAATGC 3 cut(s) 684, 922, 1213
MvaI CCWGG 4 cut(s) 72, 627, 913, 1236
MwoI GCNNNNNNNGC 3 cut(s) 399, 928, 1039
NdeI CATATG 1 cut(s) 499
NdeII GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
NlaIV GGNNCC 4 cut(s) 41, 180, 809, 1226
NmuCI GTSAC 3 cut(s) 142, 502, 1091
NsiI ATGCAT 2 cut(s) 6, 924
NspI RCATGY 4 cut(s) 8, 261, 656, 922
PaeI GCATGC 1 cut(s) 8
PceI AGGCCT 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 1270
PcsI WCGNNNNNNNCGW 2 cut(s) 190, 1101
PctI GAATGC 3 cut(s) 684, 922, 1213
PfeI GAWTC 4 cut(s) 129, 546, 647, 1159
PinAI ACCGGT 1 cut(s) 1256
PkrI GCNGC 4 cut(s) 348, 392, 691, 774
PleI GAGTC 2 cut(s) 205, 264
PpsI GAGTC 2 cut(s) 205, 264
Psp6I CCWGG 4 cut(s) 70, 625, 911, 1234
PspGI CCWGG 4 cut(s) 70, 625, 911, 1234
PspN4I GGNNCC 4 cut(s) 41, 180, 809, 1226
PspPI GGNCC 3 cut(s) 379, 405, 902
PstNI CAGNNNCTG 2 cut(s) 876, 1175
PsuI RGATCY 2 cut(s) 762, 807
RsaI GTAC 3 cut(s) 848, 917, 1226
RsaNI GTAC 3 cut(s) 847, 916, 1225
RseI CAYNNNNRTG 1 cut(s) 357
SapI GCTCTTC 1 cut(s) 1270
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 4 cut(s) 347, 391, 690, 773
Sau3AI GATC 7 cut(s) 185, 762, 807, 1096, 1140, 1154, 1297
Sau96I GGNCC 3 cut(s) 379, 405, 902
SchI GAGTC 2 cut(s) 205, 264
ScrFI CCNGG 4 cut(s) 72, 627, 913, 1236
SduI GDGCHC 1 cut(s) 615
SexAI ACCWGGT 1 cut(s) 911
SfaNI GCATC 4 cut(s) 427, 882, 896, 943
SinI GGWCC 2 cut(s) 379, 405
SmiMI CAYNNNNRTG 1 cut(s) 357
SphI GCATGC 1 cut(s) 8
Sse9I AATT 7 cut(s) 47, 66, 509, 606, 668, 1179, 1270
SseBI AGGCCT 1 cut(s) 31
SsiI CCGC 7 cut(s) 391, 642, 749, 773, 956, 1042, 1211
SspMI CTAG 2 cut(s) 768, 1302
StuI AGGCCT 1 cut(s) 31
StyD4I CCNGG 4 cut(s) 70, 625, 911, 1234
StyI CCWWGG 2 cut(s) 35, 767
TaaI ACNGT 2 cut(s) 240, 789
TaiI ACGT 2 cut(s) 688, 782
TaqI TCGA 5 cut(s) 522, 637, 926, 1104, 1157
TasI AATT 7 cut(s) 47, 66, 509, 606, 668, 1179, 1270
TatI WGTACW 1 cut(s) 846
TauI GCSGC 2 cut(s) 393, 775
TfiI GAWTC 4 cut(s) 129, 546, 647, 1159
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 21, 547
TseFI GTSAC 3 cut(s) 142, 502, 1091
TseI GCWGC 2 cut(s) 346, 689
Tsp45I GTSAC 3 cut(s) 142, 502, 1091
TspDTI ATGAA 5 cut(s) 386, 471, 501, 615, 848
TspGWI ACGGA 3 cut(s) 223, 324, 397
TspRI CASTG 2 cut(s) 21, 547
VneI GTGCAC 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 379, 405
XagI CCTNNNNNAGG 1 cut(s) 75
XapI RAATTY 3 cut(s) 66, 509, 668
XceI RCATGY 4 cut(s) 8, 261, 656, 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XmaJI CCTAGG 1 cut(s) 767
XspI CTAG 2 cut(s) 768, 1302
Zsp2I ATGCAT 2 cut(s) 6, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.